	   SnapGene      TATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAATCGATCAAGGGTTGGTTTGCGCATTCACAGTTCTCCGCAAGAATTGATTGGCTCCAATTCTTGGAGTGGTGAATCCGTTAGCGAGGTGCCGCCGGCTTCCATTCAGGTCGAGGTGGCCCGGCTCCATGCACCGCGACGCAACGCGGGGAGGCAGACAAGGTATAGGGCGGCGCCTACAATCCATGCCAACCCGTTCCATGTGCTCGCCGAGGCGGCATAAATCGCCGTGACGATCAGCGGTCCAATGATCGAAGTTAGGCTGGTAAGAGCCGCGAGCGATCCTTGAAGCTGTCCCTGATGGTCGTCATCTACCTGCCTGGACAGCATGGCCTGCAACGCGGGCATCCCGATGCCGCCGGAAGCGAGAAGAATCATAATGGGGAAGGCCATCCAGCCTCGCGTCGATCTATACCCTTCAAGCTTTGAAAAATAAACTTAATTATTATATATGTTATTTAGCTAGTTTTTTTAATTAAAGTTAAAATCGAGAGCTTGTTTGACAAAAAAACAAAAAAATTTCTTGAAAATTTTTTTTTTGACTCAATATCTAGACTTGCAAGAGCTTGGAACTTTGAGATTGTTCTAAGATGCATACAAATTCAAAATGCTTAAACAAAAATAATTTAACAAAGGAGTAAGATTGTTATGAGCCATATTCAACGGGAAACGTCTTGCTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGATCACGCATCTTCCCGACAACGCAGACCGTTCCGTGGCAAAGCAAAAGTTCAAAATCACCAACTGGTCCACCTACAACAAAGCTCTCATCAACGATATCCCGCAAGAGGCCCGGCAGTACCGGCATAACCAAGCCTATGCCTACAGCATCCAGGGTGACGGTGCCGAGGATGACGATGAGCGCATTGTTAGATTTCATACACGGTGCCTGACTGCGTTAGCAATTTAACTGTGATAAACTACCGCATTAAAGCTAATCGGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAGAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATAATCGGCATTTTCTTTTGCGTTTTTATTTGTTAACTGTTAATTGTCCTTGTTCAAGGATGCTGTCTTTGACAACAGATGTTTTCTTGCCTTTGATGTTCAGCAGGAAGCTAGGCGCAAACGTTGATTGTTTGTCTGCGTAGAATCCTCTGTTTGTCATATAGCTTGTAATCACGACATTGTTTCCTTTCGCTTGAGGTACAGCGAAGTGTGAGTAAGTAAAGGTTACATCGTTAGGATCAAGATCCATTTTTAACACAAGGCCAGTTTTGTTCAGCGGCTTGTATGGGCCAGTTAAAGAATTAGAAACATAACCAAGCATGTAAATATCGTTAGACGTAATGCCGTCAATCGTCATTTTTGATCCGCGGGAGTCAGTGAACAGATACCATTTGCCGTTCATTTTAAAGACGTTCGCGCGTTCAATTTCATCTGTTACTGTGTTAGATGCAATCAGCGGTTTCATCACTTTTTTCAGTGTGTAATCATCGTTTAGCTCAATCATACCGAGAGCGCCGTTTGCTAACTCAGCCGTGCGTTTTTTATCGCTTTGCAGAAGTTTTTGACTTTCTTGACGGAAGAATGATGTGCTTTTGCCATAGTATGCTTTGTTAAATAAAGATTCTTCGCCTTGGTAGCCATCTTCAGTTCCAGTGTTTGCTTCAAATACTAAGTATTTGTGGCCTTTATCTTCTACGTAGTGAGGATCTCTCAGCGTATGGTTGTCGCCTGAGCTGTAGTTGCCTTCATCGATGAACTGCTGTACATTTTGATACGTTTTTCCGTCACCGTCAAAGATTGATTTATAATCCTCTACACCGTTGATGTTCAAAGAGCTGTCTGATGCTGATACGTTAACTTGTGCAGTTGTCAGTGTTTGTTTGCCGTAATGTTTACCGGAGAAATCAGTGTAGAATAAACGGATTTTTCCGTCAGATGTAAATGTGGCTGAACCTGACCATTCTTGTGTTTGGTCTTTTAGGATAGAATCATTTGCATCGAATTTGTCGCTGTCTTTAAAGACGCGGCCAGCGTTTTTCCAGCTGTCAATAGAAGTTTCGCCGACTTTTTGATAGAACATGTAAATCGATGTGTCATCCGCATTTTTAGGATCTCCGGCTAATGCAAAGACGATGTGGTAGCCGTGATAGTTTGCGACAGTGCCGTCAGCGTTTTGTAATGGCCAGCTGTCCCAAACGTCCAGGCCTTTTGCAGAAGAGATATTTTTAATTGTGGACGAATCGAACTCAGGAACTTGATATTTTTCATTTTTTTGCTGTTCAGGGATTTGCAGCATATCATGGCGTGTAATATGGGAAATGCCGTATGTTTCCTTATATGGCTTTTGGTTCGTTTCTTTCGCAAACGCTTGAGTTGCGCCTCCTGCCAGCAGTGCGGTAGTAAAGGTTAATACTGTTGCTTGTTTTGCAAACTTTTTGATGTTCATCGTTCATGTCTCCTTTTTTATGTACTGTGTTAGCGGTCTGCTTCTTCCAGCCCTCCTGTTTGAAGATGGCAAGTTAGTTACGCACAATAAAAAAAGACCTAAAATATGTAAGGGGTGACGCCAAAGTATACACTTTGCCCTTTACACATTTTAGGTCTTGCCTGCTTTATCAGTAACAAACCCGCGCGATTTACTTTTCGACCTCATTCTATTAGACTCTCGTTTGGATTGCAACTGGTCTATTTTCCTCTTTTGTTTGATAGAAAATCATAAAAGGATTTGCAGACTACGGGCCTAAAGAACTAAAAAATCTATCTGTTTCTTTTCATTCTCTGTATTTTTTATAGTTTCTGTTGCATGGGCATAAAGTTGCCTTTTTAATCACAATTCAGAAAATATCATAATATCTCATTTCACTAAATAATAGTGAACGGCAGGTATATGTGATGGGTTAAAAAGGATCGATCCTCTAGCTAGAGTCGATCTTCGCCAGCAGGGCGAGGATCGTGGCATCACCGAACCGCGCCGTGCGCGGGTCGTCGGTGAGCCAGAGTTTCAGCAGGCCGCCCAGGCGGCCCAGGTCGCCATTGATGCGGGCCAGCTCGCGGACGTGCTCATAGTCCACGACGCCCGTGATTTTGTAGCCCTGGCCGACGGCCAGCAGGTAGGCCGACAGGCTCATGCCGGCTGCCGCCGCCTTTTCCTCAATCGCTCTTCGTTCGTCTGGAAGGCAGTACACCTTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGCTGCTTCCCTGCTGTTTTGTGGAATATCTACCGACTGGAAACAGGCAAATGCAGGAAATTACTGAACTGAGGGGACAGGCGAGAGACGATGCCAAAGAGCTACACCGACGAGCTGGCCGAGTGGGTTGAATCCCGCGCGGCCAAGAAGCGCCGGCGTGATGAGGCTGCGGTTGCGTTCCTGGCGGTGAGGGCGGATGTCGATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCATATTTGAATGTATTTAGAAAAATAAACAAAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACAGCCAAGCTCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCtgagttgctatagcaattttagcatcagagagattatatttttttacaagatattgaacctcactacgcgtagatgcttgagtagttgctatgcttataagtaatatagagaatgccgtaaaaaacttagtttttcttttcaaaacctttaaccatataaatttctaaagaatccttaaccgagataatagcattttatctcttataaatacagtaagttatagtatattatctaatcttagatggtagttgtttttaagttaaccctaactaaattactaggcttgcatttatatactaatatgttagaataatccactaattgaaatattggtagagggacattagtccttttaatgtaaacgtaataaactggcatttaagcgaggtgagagggtatgccaagcgttagaattaaagaaagagaaccttttgatgtcgctcttagaagatttaaaagatcttgcgaaaaggctggaatagtatctgaactacgtcgtagagaatactttgaaaagccaacttgggcacgcaaaagaaaaaaaactgccgctgttaaaagagctcataagagcaatataatagttaaaaggGCGGCCGCATACCCATACGATGTTCCAGATTATGCTTAAttaaatttaattcatcgttgagagtttaactctccctttcacaaggaattcttttttaaaatgtcacaaatttttgatcagttgactcttgatatgaaagagtcaatgaaaaataaagataaaaatagattaacaacaattcgtatggcaatatctgcgataaaacaaaagcagattgatgaaaaaatacagatcactgatgaggttgtaattgctgtaattacaaaaatgattaagcaaagacaagattcatttgatcaatacatcaaagcaaatagagctgagcttgctgaaggtgaaaaaagagagatagaaatattgactcaatacatgccaaagcaactaaatgatgaagaggttgtcgcgatagttcagaaggctatagagtctgttggagctacatcgatgaaagagatgggtaaaatcatggcaagtgtaaaaaaagaacttgctggtaggacagatatgagcaaagtaagcgctattgtgaaatctaacttagcttaaaggttgaagctttaatcagtatggcgaaaaaagtctcgaatagttttataaaagagcttgtagctacagctgatatcgttgatgttgtttcaagatatgttaacttGGTACCGAGCTCGAATTCGTAATCATGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACCCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAAC  0L  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                      /                                                      
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                          $                         
 	       	     
     &  	                   
       
        
     ( (  ) " 	  $    w   _ ^ +   7 g   =  >  >  >  B{  G  O
u I	*r)*7I"/1 #q
^4yOR/4hccccXvS 'OR[dS<;aj(!MVB}


 5BI&,Zc eDOkDjN	 y	9{(y	( r'*	
wQ-{3^yU try	C0'|o
oao
alA|o
ab	r.	Q$^ sT0ruCw0ArW	'El 6'<d,}|S\"I$]EO-W<-x		o
oaof&		'=J4 $QF
yKmqu2EOSDX\oVs6[
	',"xh6tR	moD t.  
I kOdn
kT(o

FAa g  f"\cK&			!eUCoc A$EO .
<:Z u{lZu l6'	![e
o1dpixb6 #zii hp YZ5		8	
XKeQ|&T]wI"B	#		$n	d- 0  	9F&ow}<'R	?Zo"F >~T	
		:^y~p'- 0  	9F&ow}LF,~r]	| -} x )M- 0  !p)r	T
vOX9F&o,/wS} >~T|	
&T]wI"BxW
Y<&c2i

4j'E#) 6 ($hN } %_

i M y-B<T:i
O1k- ' 		
+4.HB~	i
<OT1XkD:DQ ( 5 tR>PsX*
IWX0
:
0mD	 :PLeIOY6 ;A	Z
 f]G6vB	mP
:  k	V(i@  74Z{>'G K	 	:PLeI!p)rT
vOX,/S( 5 DtQ R Rf!b!C_fa$]K0U	MWWt\			)
u&/xU "L*sJ[	%P0t : 0XTp{X  Xp,		
0
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T
>v=0XmyI<D,X[f
<p2 l7		
dRt[inGU6v(V 
s]		(

v'.DCx)1M 
 #+y]zci7=Rs <JqTR	{x)@NZpR|"G.5@R  %?-U^	
FPgFg'			!^(2 s ~   '<AJRlRq+PT/7Rj			
E
I

{dR(x)M@>NZpt*NRV|"4%G[.inGU 6v5@Rl(V[tQ gFj>aNU8gq=Qbn'	3				Q
u
lC!/)s 6F^4:Ctv h39J#a|(h%Udz	
-|"}E Heo\	p
6X058xqyb   %?-P]VLU^|v		
} N/z&KS@T]rFPTwN%IYD"_kt7Bw * 7 >   %1?-)Rd%U^}		
$
G

'Vw
LU^FPbx0ipz@J#]O#C\X6|4Y
_BzwQV[$-DT<	P8XIM 7]oP	94+\cX}*9y	L


r
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PaZMIHcDj	U9j4VIudNaN]<zP)*DT:my^*X^XyZVEkyaYVGځj*RZ%YҪR[Nf/mNe+d'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ        >  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  6<?xml version="1.0"?><Features recycledIDs="45,44,46,43,42" nextValidID="47"><Feature recentID="9" name="P708 PriM_unst1_F1" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6173-6197" color="#50ff50" type="standard"/><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature><Feature recentID="8" name="P707 PriM_unst1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6136-6162" color="#50ff50" type="standard"/><Q name="note"><V text="P707 PriM_unst1_R1"/></Q></Feature><Feature recentID="7" name="P702 PriM_mtip1_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6886-6910" color="#50ff50" type="standard"/><Q name="note"><V text="P702 PriM_mtip1_R2"/></Q></Feature><Feature recentID="3" name="P697 Ck_PriM_mpk1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6131" color="#50ff50" type="standard"/><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature><Feature recentID="0" name="P698 Ck_PriM_mpk1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6584-6606" color="#50ff50" type="standard"/><Q name="note"><V text="P698 Ck_PriM_mpk1R"/></Q></Feature><Feature recentID="41" name="P694 PriM_mpk_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7204-7231" color="#50ff50" type="standard"/><Q name="note"><V text="P694 PriM_mpk_R2"/></Q></Feature><Feature recentID="40" name="P693 PriM_mpk_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6194-6222" color="#50ff50" type="standard"/><Q name="note"><V text="P693 PriM_mpk_F2"/></Q></Feature><Feature recentID="39" name="P692 PriM_mpk_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6143-6169" color="#50ff50" type="standard"/><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="25" name="P444 pKL75_F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6431-6457" color="#50ff50" type="standard"/><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="24" name="P440 RT_FTL0702-3R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6391-6410" color="#50ff50" type="standard"/><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="23" name="P439 RT_FTL0702-3F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6325-6346" color="#50ff50" type="standard"/><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="22" name="P438 RT_FTL0702-2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6846-6867" color="#50ff50" type="standard"/><Q name="note"><V text="P438 RT_FTL0702-2R"/></Q></Feature><Feature recentID="21" name="P437 RT_FTL0702-2F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6741-6763" color="#50ff50" type="standard"/><Q name="note"><V text="P437 RT_FTL0702-2F"/></Q></Feature><Feature recentID="20" name="P436 RT_FTL0702-1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6206-6227" color="#50ff50" type="standard"/><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="19" name="P435 RT_FTL0702-1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6127" color="#50ff50" type="standard"/><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="18" name="P433 FTL0702cmp_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6946-6973" color="#50ff50" type="standard"/><Q name="note"><V text="P433 FTL0702cmp_R2"/></Q></Feature><Feature recentID="17" name="P428 FTL0702_VSVG_2F" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6943-6970" color="#50ff50" type="standard"/><Q name="note"><V text="P428 FTL0702_VSVG_2F"/></Q></Feature><Feature recentID="16" name="P411 FTL0702sp_2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6594-6618" color="#50ff50" type="standard"/><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="15" name="P368 FTL0702seqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6663-6691" color="#8080ff" type="standard"/><Q name="note"><V text="P368 FTL0702seqR1"/></Q></Feature><Feature recentID="14" name="P365 FTL0702cmp_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6943-6970" color="#8080ff" type="standard"/><Q name="note"><V text="P365 FTL0702cmp_R"/></Q></Feature><Feature recentID="13" name="hypothetical protein" directionality="1" translationMW="31542.95" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="199" hitsStopCodon="1"><Segment range="6108-6973" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="12" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6973" color="#00e600" type="standard"/></Feature><Feature recentID="11" name="P427 FTL0702_VSVG_1R" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6974-7003" color="#50ff50" type="standard"/><Q name="note"><V text="P427 FTL0702_VSVG_1R"/></Q></Feature><Feature recentID="10" name="PRP11" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7098-7128" color="#8080ff" type="standard"/><Q name="note"><V text="PRP11"/></Q></Feature><Feature recentID="6" name="P329 SP_D0702-R" directionality="1" type="primer_bind" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7064-7091" color="#8080ff" type="standard"/><Q name="note"><V text="P329 SP_D0702-R"/></Q></Feature><Feature recentID="5" name="RPp7" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6964-6992" color="#0080ff" type="standard"/><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="4" name="FAD binding family protein" directionality="2" translationMW="7383.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="527" hitsStopCodon="1"><Segment range="7038-7231" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="2" name="FTL_0701" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7038-7231" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-7231" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="38" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7960" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="mol_type"><V predef="genomic DNA"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="37" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7232-7236" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="36" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7666-288" color="#ffff00" type="standard"/><Q name="note"><V text="ColE origin"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="35" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="34" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="32" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="30" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7258-7274" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  Ut<?xml version="1.0"?><Primers recycledIDs="24,23,30,29,25,37,12" nextValidID="40"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="26" name="P821 PriM_mtip1_R3" sequence="atatGGTACCgtaactttggtattagttctgatactg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6438-6464" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6438-6464" bases="gtaactttggtattagttctgatactg"/></BindingSite><BindingSite simplified="1" location="6438-6464" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6438-6464" bases="gtaactttggtattagttctgatactg"/></BindingSite></Primer><Primer recentID="1" name="P819 PriM_mpk_R2" sequence="atatGGTACCtgtaaagccaaatatactttctcttcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6897-6923" boundStrand="1" annealedBases="tgtaaagccaaatatactttctcttcg" meltingTemperature="54"><Component bases="atatGGTACC"/><Component hybridizedRange="6897-6923" bases="tgtaaagccaaatatactttctcttcg"/></BindingSite><BindingSite simplified="1" location="6897-6923" boundStrand="1" annealedBases="tgtaaagccaaatatactttctcttcg" meltingTemperature="54"><Component bases="atatGGTACC"/><Component hybridizedRange="6897-6923" bases="tgtaaagccaaatatactttctcttcg"/></BindingSite></Primer><Primer recentID="21" name="P707 PriM_unst1_R1" sequence="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6135-6181" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6172-6181" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6163-6168" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6135-6161" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite><BindingSite simplified="1" location="6135-6181" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6172-6181" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6163-6168" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6135-6161" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite></Primer><Primer recentID="28" name="P708 PriM_unst1_F1" sequence="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6152-6196" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6152-6161" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6163-6168" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6172-6196" bases="ggtgcaagagtagttctttggaatg"/></BindingSite><BindingSite simplified="1" location="6152-6196" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6152-6161" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6163-6168" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6172-6196" bases="ggtgcaagagtagttctttggaatg"/></BindingSite></Primer><Primer recentID="27" name="P428 FTL0702_VSVG_2F" sequence="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGCttcttctttaggcaaaacattacttaag" description="0"><BindingSite location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="39" name="P427 FTL0702_VSVG_1R" sequence="GATATTGAAATGAATAGATTAGGTAAATAAactaatgactaaatttctaaaattaattag" description="0"><BindingSite location="6972-7002" boundStrand="0" annealedBases="Aactaatgactaaatttctaaaattaattag" meltingTemperature="51"><Component bases="GATATTGAAATGAATAGATTAGGTAAATA"/><Component hybridizedRange="6972-7002" bases="Aactaatgactaaatttctaaaattaattag"/></BindingSite><BindingSite simplified="1" location="6972-7002" boundStrand="0" annealedBases="Aactaatgactaaatttctaaaattaattag" meltingTemperature="51"><Component bases="GATATTGAAATGAATAGATTAGGTAAATA"/><Component hybridizedRange="6972-7002" bases="Aactaatgactaaatttctaaaattaattag"/></BindingSite></Primer><Primer recentID="38" name="P693 PriM_mpk_F2" sequence="GCAggtgcaGCAgtagttcttGCAaatggtcaatataacatcaatagcaaaga" description="0"><BindingSite location="6172-6221" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6172-6177" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6180-6189" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6193-6221" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite><BindingSite simplified="1" location="6172-6221" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6172-6177" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6180-6189" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6193-6221" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite></Primer><Primer recentID="36" name="P692 PriM_mpk_R1" sequence="TGCaagaactacTGCtgcaccTGCgaacatatcaatgttgcttctgtagtc" description="0"><BindingSite location="6142-6189" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6180-6189" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6172-6177" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6142-6168" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite><BindingSite simplified="1" location="6142-6189" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6180-6189" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6172-6177" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6142-6168" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite></Primer><Primer recentID="35" name="P365 FTL0702cmp_R1" sequence="atatGCGGCCGCttcttctttaggcaaaacattacttaag" description="Contains NotI site"><BindingSite location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="34" name="P694 PriM_mpk_R2" sequence="GGTACCaagctatgaacatgattatagctttagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7203-7236" boundStrand="1" annealedBases="GGTACCaagctatgaacatgattatagctttagc" meltingTemperature="60"><Component hybridizedRange="7203-7236" bases="GGTACCaagctatgaacatgattatagctttagc"/></BindingSite><BindingSite simplified="1" location="7203-7236" boundStrand="1" annealedBases="GGTACCaagctatgaacatgattatagctttagc" meltingTemperature="60"><Component hybridizedRange="7203-7236" bases="GGTACCaagctatgaacatgattatagctttagc"/></BindingSite></Primer><Primer recentID="33" name="P433 FTL0702cmp_R2" sequence="atatGGATCCtcattcttctttaggcaaaacattactt" description="Contains BamHI site"><BindingSite location="6945-6972" boundStrand="1" annealedBases="tcattcttctttaggcaaaacattactt" meltingTemperature="55"><Component bases="atatGGATCC"/><Component hybridizedRange="6945-6972" bases="tcattcttctttaggcaaaacattactt"/></BindingSite><BindingSite simplified="1" location="6945-6972" boundStrand="1" annealedBases="tcattcttctttaggcaaaacattactt" meltingTemperature="55"><Component bases="atatGGATCC"/><Component hybridizedRange="6945-6972" bases="tcattcttctttaggcaaaacattactt"/></BindingSite></Primer><Primer recentID="32" name="P366 FTL0702cmp_R2" sequence="atatGGATCCttcttctttaggcaaaacattacttaag" description="&quot;Contains BamHI site, could use to make vector lacking VSVG tag&quot;"><BindingSite location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGGATCC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGGATCC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="2" name="P444 pKL75_F" sequence="atatGGTACCagcttaatcagtatcagaactaatacc" description="Contains KpnI site"><BindingSite location="6430-6456" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6430-6456" bases="agcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6430-6456" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6430-6456" bases="agcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="8" name="P695 PriM_mpk_R3" sequence="catcagcTGCtagtgaaactgctttagttgtatcact" description="0"><BindingSite location="6295-6331" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6325-6331" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6295-6321" bases="tagtgaaactgctttagttgtatcact"/></BindingSite><BindingSite simplified="1" location="6295-6331" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6325-6331" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6295-6321" bases="tagtgaaactgctttagttgtatcact"/></BindingSite></Primer><Primer recentID="31" name="P696 PriM_mpk_F4" sequence="ttcactaGCAgctgatgctaacacacttacatatac" description="0"><BindingSite location="6325-6350" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6325-6350" bases="gctgatgctaacacacttacatatac"/></BindingSite><BindingSite simplified="1" location="6325-6350" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6325-6350" bases="gctgatgctaacacacttacatatac"/></BindingSite></Primer><Primer recentID="22" name="P702 PriM_mtip1_R2" sequence="atatGGTACCtactttctcttcgaaaacggtgtcg" description="Contains KpnI site"><BindingSite location="6885-6909" boundStrand="1" annealedBases="tactttctcttcgaaaacggtgtcg" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6885-6909" bases="tactttctcttcgaaaacggtgtcg"/></BindingSite><BindingSite simplified="1" location="6885-6909" boundStrand="1" annealedBases="tactttctcttcgaaaacggtgtcg" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6885-6909" bases="tactttctcttcgaaaacggtgtcg"/></BindingSite></Primer><Primer recentID="0" name="P411 FTL0702sp_2R" sequence="atatGGTACCgcatttgtagctgctttaagtgccc" description="Contains KpnI site"><BindingSite location="6593-6617" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="6593-6617" bases="gcatttgtagctgctttaagtgccc"/></BindingSite><BindingSite simplified="1" location="6593-6617" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="6593-6617" bases="gcatttgtagctgctttaagtgccc"/></BindingSite></Primer><Primer recentID="3" name="P368 FTL0702seqR1" sequence="acttgtagatattaacattaaagtatgac" description="0"><BindingSite location="6662-6690" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="acttgtagatattaacattaaagtatgac"/></BindingSite><BindingSite simplified="1" location="6662-6690" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="acttgtagatattaacattaaagtatgac"/></BindingSite></Primer><Primer recentID="4" name="P430 CkFTL0702V_R" sequence="gtcatactttaatgttaatatctacaagt" description="0"><BindingSite location="6662-6690" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite><BindingSite simplified="1" location="6662-6690" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite></Primer><Primer recentID="5" name="P329 SP_D0702-R" sequence="aatgatgacaataaaatagaccatttgg" description="0"><BindingSite location="7063-7090" boundStrand="0" annealedBases="aatgatgacaataaaatagaccatttgg" meltingTemperature="55"><Component hybridizedRange="7063-7090" bases="aatgatgacaataaaatagaccatttgg"/></BindingSite><BindingSite simplified="1" location="7063-7090" boundStrand="0" annealedBases="aatgatgacaataaaatagaccatttgg" meltingTemperature="55"><Component hybridizedRange="7063-7090" bases="aatgatgacaataaaatagaccatttgg"/></BindingSite></Primer><Primer recentID="6" name="P697 Ck_PriM_mpk1F" sequence="cagcatgggattctgctactagtc" description="0"><BindingSite location="6107-6130" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="58"><Component hybridizedRange="6107-6130" bases="cagcatgggattctgctactagtc"/></BindingSite><BindingSite simplified="1" location="6107-6130" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="58"><Component hybridizedRange="6107-6130" bases="cagcatgggattctgctactagtc"/></BindingSite></Primer><Primer recentID="7" name="P437 RT_FTL0702-2F" sequence="agcaagtaagtgatgctgataga" description="0"><BindingSite location="6740-6762" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="6740-6762" bases="agcaagtaagtgatgctgataga"/></BindingSite><BindingSite simplified="1" location="6740-6762" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="6740-6762" bases="agcaagtaagtgatgctgataga"/></BindingSite></Primer><Primer recentID="9" name="P698 Ck_PriM_mpk1R" sequence="gctttaagtgcccagttgtcgcc" description="0"><BindingSite location="6583-6605" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="6583-6605" bases="gctttaagtgcccagttgtcgcc"/></BindingSite><BindingSite simplified="1" location="6583-6605" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="6583-6605" bases="gctttaagtgcccagttgtcgcc"/></BindingSite></Primer><Primer recentID="10" name="P438 RT_FTL0702-2R" sequence="agttgtccggctatctgtttag" description="0"><BindingSite location="6845-6866" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="6845-6866" bases="agttgtccggctatctgtttag"/></BindingSite><BindingSite simplified="1" location="6845-6866" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="6845-6866" bases="agttgtccggctatctgtttag"/></BindingSite></Primer><Primer recentID="11" name="P439 RT_FTL0702-3F" sequence="tgctgatgctaacacacttaca" description="0"><BindingSite location="6324-6345" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6324-6345" bases="tgctgatgctaacacacttaca"/></BindingSite><BindingSite simplified="1" location="6324-6345" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6324-6345" bases="tgctgatgctaacacacttaca"/></BindingSite></Primer><Primer recentID="13" name="P436 RT_FTL0702-1R" sequence="cggcatctttgctattgatgtt" description="0"><BindingSite location="6205-6226" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6205-6226" bases="cggcatctttgctattgatgtt"/></BindingSite><BindingSite simplified="1" location="6205-6226" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6205-6226" bases="cggcatctttgctattgatgtt"/></BindingSite></Primer><Primer recentID="14" name="P440 RT_FTL0702-3R" sequence="ccgttcttggcgaagctatt" description="0"><BindingSite location="6390-6409" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6390-6409" bases="ccgttcttggcgaagctatt"/></BindingSite><BindingSite simplified="1" location="6390-6409" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6390-6409" bases="ccgttcttggcgaagctatt"/></BindingSite></Primer><Primer recentID="15" name="P435 RT_FTL0702-1F" sequence="cagcatgggattctgctact" description="0"><BindingSite location="6107-6126" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6107-6126" bases="cagcatgggattctgctact"/></BindingSite><BindingSite simplified="1" location="6107-6126" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6107-6126" bases="cagcatgggattctgctact"/></BindingSite></Primer><Primer recentID="16" name="KROL20 PriMnoC1FR1R" sequence="aattgatagagccgttcttggcgaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6396-6420" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6396-6420" bases="aattgatagagccgttcttggcgaa"/></BindingSite><BindingSite simplified="1" location="6396-6420" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6396-6420" bases="aattgatagagccgttcttggcgaa"/></BindingSite></Primer><Primer recentID="17" name="KROL21 PriMnoC1FR2F" sequence="atatGCtgaagctagcttaatcagtatcagaactaatacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6423-6456" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="58"><Component bases="atatGC"/><Component hybridizedRange="6423-6456" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6423-6456" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="58"><Component bases="atatGC"/><Component hybridizedRange="6423-6456" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="18" name="KROL23 PriMnoC2FR2F" sequence="aaaaatgcaGCAaattttacagcagctaaaaatagtaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6775-6814" boundStrand="0" annealedBases="aaaaatgcaGCaattttacagcagctaaaaatagtaaca" meltingTemperature="59"><Component hybridizedRange="6775-6785" bases="aaaaatgcaGC"/><Component bases="A"/><Component hybridizedRange="6787-6814" bases="aattttacagcagctaaaaatagtaaca"/></BindingSite><BindingSite simplified="1" location="6775-6814" boundStrand="0" annealedBases="aaaaatgcaGCaattttacagcagctaaaaatagtaaca" meltingTemperature="59"><Component hybridizedRange="6775-6785" bases="aaaaatgcaGC"/><Component bases="A"/><Component hybridizedRange="6787-6814" bases="aattttacagcagctaaaaatagtaaca"/></BindingSite></Primer><Primer recentID="19" name="KROL22 PriMnoC2FR1R" sequence="tgtaaaattaGCAgcatttttaccaaatagaactctatca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6756-6795" boundStrand="1" annealedBases="tgtaaaattaGCgcatttttaccaaatagaactctatca" meltingTemperature="56"><Component hybridizedRange="6784-6795" bases="tgtaaaattaGC"/><Component bases="A"/><Component hybridizedRange="6756-6782" bases="gcatttttaccaaatagaactctatca"/></BindingSite><BindingSite simplified="1" location="6756-6795" boundStrand="1" annealedBases="tgtaaaattaGCgcatttttaccaaatagaactctatca" meltingTemperature="56"><Component hybridizedRange="6784-6795" bases="tgtaaaattaGC"/><Component bases="A"/><Component hybridizedRange="6756-6782" bases="gcatttttaccaaatagaactctatca"/></BindingSite></Primer><Primer recentID="20" name="KROL24 PriMnoC2FR2R" sequence="atatGGTACCtgtcgaaagtgattcttagattcagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7002-7027" boundStrand="1" annealedBases="tgtcgaaagtgattcttagattcagc" meltingTemperature="56"><Component bases="atatGGTACC"/><Component hybridizedRange="7002-7027" bases="tgtcgaaagtgattcttagattcagc"/></BindingSite><BindingSite simplified="1" location="7002-7027" boundStrand="1" annealedBases="tgtcgaaagtgattcttagattcagc" meltingTemperature="56"><Component bases="atatGGTACC"/><Component hybridizedRange="7002-7027" bases="tgtcgaaagtgattcttagattcagc"/></BindingSite></Primer></Primers>
  D<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="21:33:19">2018.12.17</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAMky.E5I+Ymd=j\f^zkGR	!ySJٝ%zRHU֢m&yg)NEf]mYUN%_m^Ii1׀ޕ4V5mu&yXI&
PaZMIHcDj	U5j4VIudNaN]<zP)*DT:my^*X^XyZVEkyaYVGځj*RZ%YҪR[Nf/mNe+d'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ        >  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  6<?xml version="1.0"?><Features recycledIDs="45,44,46,43,42" nextValidID="47"><Feature recentID="9" name="P708 PriM_unst1_F1" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6173-6197" color="#50ff50" type="standard"/><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature><Feature recentID="8" name="P707 PriM_unst1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6136-6162" color="#50ff50" type="standard"/><Q name="note"><V text="P707 PriM_unst1_R1"/></Q></Feature><Feature recentID="7" name="P702 PriM_mtip1_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6886-6910" color="#50ff50" type="standard"/><Q name="note"><V text="P702 PriM_mtip1_R2"/></Q></Feature><Feature recentID="3" name="P697 Ck_PriM_mpk1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6131" color="#50ff50" type="standard"/><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature><Feature recentID="0" name="P698 Ck_PriM_mpk1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6584-6606" color="#50ff50" type="standard"/><Q name="note"><V text="P698 Ck_PriM_mpk1R"/></Q></Feature><Feature recentID="41" name="P694 PriM_mpk_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7204-7231" color="#50ff50" type="standard"/><Q name="note"><V text="P694 PriM_mpk_R2"/></Q></Feature><Feature recentID="40" name="P693 PriM_mpk_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6194-6222" color="#50ff50" type="standard"/><Q name="note"><V text="P693 PriM_mpk_F2"/></Q></Feature><Feature recentID="39" name="P692 PriM_mpk_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6143-6169" color="#50ff50" type="standard"/><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="25" name="P444 pKL75_F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6431-6457" color="#50ff50" type="standard"/><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="24" name="P440 RT_FTL0702-3R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6391-6410" color="#50ff50" type="standard"/><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="23" name="P439 RT_FTL0702-3F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6325-6346" color="#50ff50" type="standard"/><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="22" name="P438 RT_FTL0702-2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6846-6867" color="#50ff50" type="standard"/><Q name="note"><V text="P438 RT_FTL0702-2R"/></Q></Feature><Feature recentID="21" name="P437 RT_FTL0702-2F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6741-6763" color="#50ff50" type="standard"/><Q name="note"><V text="P437 RT_FTL0702-2F"/></Q></Feature><Feature recentID="20" name="P436 RT_FTL0702-1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6206-6227" color="#50ff50" type="standard"/><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="19" name="P435 RT_FTL0702-1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6127" color="#50ff50" type="standard"/><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="18" name="P433 FTL0702cmp_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6946-6973" color="#50ff50" type="standard"/><Q name="note"><V text="P433 FTL0702cmp_R2"/></Q></Feature><Feature recentID="17" name="P428 FTL0702_VSVG_2F" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6943-6970" color="#50ff50" type="standard"/><Q name="note"><V text="P428 FTL0702_VSVG_2F"/></Q></Feature><Feature recentID="16" name="P411 FTL0702sp_2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6594-6618" color="#50ff50" type="standard"/><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="15" name="P368 FTL0702seqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6663-6691" color="#8080ff" type="standard"/><Q name="note"><V text="P368 FTL0702seqR1"/></Q></Feature><Feature recentID="14" name="P365 FTL0702cmp_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6943-6970" color="#8080ff" type="standard"/><Q name="note"><V text="P365 FTL0702cmp_R"/></Q></Feature><Feature recentID="13" name="hypothetical protein" directionality="1" translationMW="31542.95" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="199" hitsStopCodon="1"><Segment range="6108-6973" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="12" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6973" color="#00e600" type="standard"/></Feature><Feature recentID="11" name="P427 FTL0702_VSVG_1R" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6974-7003" color="#50ff50" type="standard"/><Q name="note"><V text="P427 FTL0702_VSVG_1R"/></Q></Feature><Feature recentID="10" name="PRP11" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7098-7128" color="#8080ff" type="standard"/><Q name="note"><V text="PRP11"/></Q></Feature><Feature recentID="6" name="P329 SP_D0702-R" directionality="1" type="primer_bind" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7064-7091" color="#8080ff" type="standard"/><Q name="note"><V text="P329 SP_D0702-R"/></Q></Feature><Feature recentID="5" name="RPp7" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6964-6992" color="#0080ff" type="standard"/><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="4" name="FAD binding family protein" directionality="2" translationMW="7383.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="527" hitsStopCodon="1"><Segment range="7038-7231" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="2" name="FTL_0701" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7038-7231" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-7231" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="38" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7960" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="mol_type"><V predef="genomic DNA"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="37" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7232-7236" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="36" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7666-288" color="#ffff00" type="standard"/><Q name="note"><V text="ColE origin"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="35" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="34" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="32" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="30" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7258-7274" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  Ut<?xml version="1.0"?><Primers recycledIDs="24,23,30,29,25,37,12" nextValidID="40"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="26" name="P821 PriM_mtip1_R3" sequence="atatGGTACCgtaactttggtattagttctgatactg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6438-6464" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6438-6464" bases="gtaactttggtattagttctgatactg"/></BindingSite><BindingSite simplified="1" location="6438-6464" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6438-6464" bases="gtaactttggtattagttctgatactg"/></BindingSite></Primer><Primer recentID="1" name="P819 PriM_mpk_R2" sequence="atatGGTACCtgtaaagccaaatatactttctcttcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6897-6923" boundStrand="1" annealedBases="tgtaaagccaaatatactttctcttcg" meltingTemperature="54"><Component bases="atatGGTACC"/><Component hybridizedRange="6897-6923" bases="tgtaaagccaaatatactttctcttcg"/></BindingSite><BindingSite simplified="1" location="6897-6923" boundStrand="1" annealedBases="tgtaaagccaaatatactttctcttcg" meltingTemperature="54"><Component bases="atatGGTACC"/><Component hybridizedRange="6897-6923" bases="tgtaaagccaaatatactttctcttcg"/></BindingSite></Primer><Primer recentID="21" name="P707 PriM_unst1_R1" sequence="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6135-6181" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6172-6181" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6163-6168" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6135-6161" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite><BindingSite simplified="1" location="6135-6181" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6172-6181" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6163-6168" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6135-6161" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite></Primer><Primer recentID="28" name="P708 PriM_unst1_F1" sequence="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6152-6196" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6152-6161" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6163-6168" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6172-6196" bases="ggtgcaagagtagttctttggaatg"/></BindingSite><BindingSite simplified="1" location="6152-6196" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6152-6161" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6163-6168" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6172-6196" bases="ggtgcaagagtagttctttggaatg"/></BindingSite></Primer><Primer recentID="27" name="P428 FTL0702_VSVG_2F" sequence="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGCttcttctttaggcaaaacattacttaag" description="0"><BindingSite location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="39" name="P427 FTL0702_VSVG_1R" sequence="GATATTGAAATGAATAGATTAGGTAAATAAactaatgactaaatttctaaaattaattag" description="0"><BindingSite location="6972-7002" boundStrand="0" annealedBases="Aactaatgactaaatttctaaaattaattag" meltingTemperature="51"><Component bases="GATATTGAAATGAATAGATTAGGTAAATA"/><Component hybridizedRange="6972-7002" bases="Aactaatgactaaatttctaaaattaattag"/></BindingSite><BindingSite simplified="1" location="6972-7002" boundStrand="0" annealedBases="Aactaatgactaaatttctaaaattaattag" meltingTemperature="51"><Component bases="GATATTGAAATGAATAGATTAGGTAAATA"/><Component hybridizedRange="6972-7002" bases="Aactaatgactaaatttctaaaattaattag"/></BindingSite></Primer><Primer recentID="38" name="P693 PriM_mpk_F2" sequence="GCAggtgcaGCAgtagttcttGCAaatggtcaatataacatcaatagcaaaga" description="0"><BindingSite location="6172-6221" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6172-6177" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6180-6189" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6193-6221" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite><BindingSite simplified="1" location="6172-6221" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6172-6177" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6180-6189" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6193-6221" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite></Primer><Primer recentID="36" name="P692 PriM_mpk_R1" sequence="TGCaagaactacTGCtgcaccTGCgaacatatcaatgttgcttctgtagtc" description="0"><BindingSite location="6142-6189" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6180-6189" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6172-6177" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6142-6168" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite><BindingSite simplified="1" location="6142-6189" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6180-6189" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6172-6177" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6142-6168" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite></Primer><Primer recentID="35" name="P365 FTL0702cmp_R1" sequence="atatGCGGCCGCttcttctttaggcaaaacattacttaag" description="Contains NotI site"><BindingSite location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="34" name="P694 PriM_mpk_R2" sequence="GGTACCaagctatgaacatgattatagctttagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7203-7236" boundStrand="1" annealedBases="GGTACCaagctatgaacatgattatagctttagc" meltingTemperature="60"><Component hybridizedRange="7203-7236" bases="GGTACCaagctatgaacatgattatagctttagc"/></BindingSite><BindingSite simplified="1" location="7203-7236" boundStrand="1" annealedBases="GGTACCaagctatgaacatgattatagctttagc" meltingTemperature="60"><Component hybridizedRange="7203-7236" bases="GGTACCaagctatgaacatgattatagctttagc"/></BindingSite></Primer><Primer recentID="33" name="P433 FTL0702cmp_R2" sequence="atatGGATCCtcattcttctttaggcaaaacattactt" description="Contains BamHI site"><BindingSite location="6945-6972" boundStrand="1" annealedBases="tcattcttctttaggcaaaacattactt" meltingTemperature="55"><Component bases="atatGGATCC"/><Component hybridizedRange="6945-6972" bases="tcattcttctttaggcaaaacattactt"/></BindingSite><BindingSite simplified="1" location="6945-6972" boundStrand="1" annealedBases="tcattcttctttaggcaaaacattactt" meltingTemperature="55"><Component bases="atatGGATCC"/><Component hybridizedRange="6945-6972" bases="tcattcttctttaggcaaaacattactt"/></BindingSite></Primer><Primer recentID="32" name="P366 FTL0702cmp_R2" sequence="atatGGATCCttcttctttaggcaaaacattacttaag" description="&quot;Contains BamHI site, could use to make vector lacking VSVG tag&quot;"><BindingSite location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGGATCC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGGATCC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="2" name="P444 pKL75_F" sequence="atatGGTACCagcttaatcagtatcagaactaatacc" description="Contains KpnI site"><BindingSite location="6430-6456" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6430-6456" bases="agcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6430-6456" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6430-6456" bases="agcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="8" name="P695 PriM_mpk_R3" sequence="catcagcTGCtagtgaaactgctttagttgtatcact" description="0"><BindingSite location="6295-6331" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6325-6331" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6295-6321" bases="tagtgaaactgctttagttgtatcact"/></BindingSite><BindingSite simplified="1" location="6295-6331" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6325-6331" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6295-6321" bases="tagtgaaactgctttagttgtatcact"/></BindingSite></Primer><Primer recentID="31" name="P696 PriM_mpk_F4" sequence="ttcactaGCAgctgatgctaacacacttacatatac" description="0"><BindingSite location="6325-6350" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6325-6350" bases="gctgatgctaacacacttacatatac"/></BindingSite><BindingSite simplified="1" location="6325-6350" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6325-6350" bases="gctgatgctaacacacttacatatac"/></BindingSite></Primer><Primer recentID="22" name="P702 PriM_mtip1_R2" sequence="atatGGTACCtactttctcttcgaaaacggtgtcg" description="Contains KpnI site"><BindingSite location="6885-6909" boundStrand="1" annealedBases="tactttctcttcgaaaacggtgtcg" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6885-6909" bases="tactttctcttcgaaaacggtgtcg"/></BindingSite><BindingSite simplified="1" location="6885-6909" boundStrand="1" annealedBases="tactttctcttcgaaaacggtgtcg" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6885-6909" bases="tactttctcttcgaaaacggtgtcg"/></BindingSite></Primer><Primer recentID="0" name="P411 FTL0702sp_2R" sequence="atatGGTACCgcatttgtagctgctttaagtgccc" description="Contains KpnI site"><BindingSite location="6593-6617" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="6593-6617" bases="gcatttgtagctgctttaagtgccc"/></BindingSite><BindingSite simplified="1" location="6593-6617" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="6593-6617" bases="gcatttgtagctgctttaagtgccc"/></BindingSite></Primer><Primer recentID="3" name="P368 FTL0702seqR1" sequence="acttgtagatattaacattaaagtatgac" description="0"><BindingSite location="6662-6690" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="acttgtagatattaacattaaagtatgac"/></BindingSite><BindingSite simplified="1" location="6662-6690" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="acttgtagatattaacattaaagtatgac"/></BindingSite></Primer><Primer recentID="4" name="P430 CkFTL0702V_R" sequence="gtcatactttaatgttaatatctacaagt" description="0"><BindingSite location="6662-6690" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite><BindingSite simplified="1" location="6662-6690" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite></Primer><Primer recentID="5" name="P329 SP_D0702-R" sequence="aatgatgacaataaaatagaccatttgg" description="0"><BindingSite location="7063-7090" boundStrand="0" annealedBases="aatgatgacaataaaatagaccatttgg" meltingTemperature="55"><Component hybridizedRange="7063-7090" bases="aatgatgacaataaaatagaccatttgg"/></BindingSite><BindingSite simplified="1" location="7063-7090" boundStrand="0" annealedBases="aatgatgacaataaaatagaccatttgg" meltingTemperature="55"><Component hybridizedRange="7063-7090" bases="aatgatgacaataaaatagaccatttgg"/></BindingSite></Primer><Primer recentID="6" name="P697 Ck_PriM_mpk1F" sequence="cagcatgggattctgctactagtc" description="0"><BindingSite location="6107-6130" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="58"><Component hybridizedRange="6107-6130" bases="cagcatgggattctgctactagtc"/></BindingSite><BindingSite simplified="1" location="6107-6130" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="58"><Component hybridizedRange="6107-6130" bases="cagcatgggattctgctactagtc"/></BindingSite></Primer><Primer recentID="7" name="P437 RT_FTL0702-2F" sequence="agcaagtaagtgatgctgataga" description="0"><BindingSite location="6740-6762" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="6740-6762" bases="agcaagtaagtgatgctgataga"/></BindingSite><BindingSite simplified="1" location="6740-6762" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="6740-6762" bases="agcaagtaagtgatgctgataga"/></BindingSite></Primer><Primer recentID="9" name="P698 Ck_PriM_mpk1R" sequence="gctttaagtgcccagttgtcgcc" description="0"><BindingSite location="6583-6605" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="6583-6605" bases="gctttaagtgcccagttgtcgcc"/></BindingSite><BindingSite simplified="1" location="6583-6605" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="6583-6605" bases="gctttaagtgcccagttgtcgcc"/></BindingSite></Primer><Primer recentID="10" name="P438 RT_FTL0702-2R" sequence="agttgtccggctatctgtttag" description="0"><BindingSite location="6845-6866" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="6845-6866" bases="agttgtccggctatctgtttag"/></BindingSite><BindingSite simplified="1" location="6845-6866" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="6845-6866" bases="agttgtccggctatctgtttag"/></BindingSite></Primer><Primer recentID="11" name="P439 RT_FTL0702-3F" sequence="tgctgatgctaacacacttaca" description="0"><BindingSite location="6324-6345" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6324-6345" bases="tgctgatgctaacacacttaca"/></BindingSite><BindingSite simplified="1" location="6324-6345" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6324-6345" bases="tgctgatgctaacacacttaca"/></BindingSite></Primer><Primer recentID="13" name="P436 RT_FTL0702-1R" sequence="cggcatctttgctattgatgtt" description="0"><BindingSite location="6205-6226" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6205-6226" bases="cggcatctttgctattgatgtt"/></BindingSite><BindingSite simplified="1" location="6205-6226" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6205-6226" bases="cggcatctttgctattgatgtt"/></BindingSite></Primer><Primer recentID="14" name="P440 RT_FTL0702-3R" sequence="ccgttcttggcgaagctatt" description="0"><BindingSite location="6390-6409" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6390-6409" bases="ccgttcttggcgaagctatt"/></BindingSite><BindingSite simplified="1" location="6390-6409" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6390-6409" bases="ccgttcttggcgaagctatt"/></BindingSite></Primer><Primer recentID="15" name="P435 RT_FTL0702-1F" sequence="cagcatgggattctgctact" description="0"><BindingSite location="6107-6126" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6107-6126" bases="cagcatgggattctgctact"/></BindingSite><BindingSite simplified="1" location="6107-6126" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6107-6126" bases="cagcatgggattctgctact"/></BindingSite></Primer><Primer recentID="16" name="KROL20 PriMnoC1FR1R" sequence="aattgatagagccgttcttggcgaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6396-6420" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6396-6420" bases="aattgatagagccgttcttggcgaa"/></BindingSite><BindingSite simplified="1" location="6396-6420" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6396-6420" bases="aattgatagagccgttcttggcgaa"/></BindingSite></Primer><Primer recentID="17" name="KROL21 PriMnoC1FR2F" sequence="atatGCtgaagctagcttaatcagtatcagaactaatacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6423-6456" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="58"><Component bases="atatGC"/><Component hybridizedRange="6423-6456" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6423-6456" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="58"><Component bases="atatGC"/><Component hybridizedRange="6423-6456" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="18" name="KROL23 PriMnoC2FR2F" sequence="aaaaatgcaGCAaattttacagcagctaaaaatagtaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6775-6814" boundStrand="0" annealedBases="aaaaatgcaGCAaattttacagcagctaaaaatagtaaca" meltingTemperature="62"><Component hybridizedRange="6775-6814" bases="aaaaatgcaGCAaattttacagcagctaaaaatagtaaca"/></BindingSite><BindingSite simplified="1" location="6775-6814" boundStrand="0" annealedBases="aaaaatgcaGCAaattttacagcagctaaaaatagtaaca" meltingTemperature="62"><Component hybridizedRange="6775-6814" bases="aaaaatgcaGCAaattttacagcagctaaaaatagtaaca"/></BindingSite></Primer><Primer recentID="19" name="KROL22 PriMnoC2FR1R" sequence="tgtaaaattaGCAgcatttttaccaaatagaactctatca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6756-6795" boundStrand="1" annealedBases="tgtaaaattGCgcatttttaccaaatagaactctatca" meltingTemperature="53"><Component hybridizedRange="6787-6795" bases="tgtaaaatt"/><Component bases="a"/><Component hybridizedRange="6784-6785" bases="GC"/><Component bases="A"/><Component hybridizedRange="6756-6782" bases="gcatttttaccaaatagaactctatca"/></BindingSite><BindingSite simplified="1" location="6756-6795" boundStrand="1" annealedBases="tgtaaaattGCgcatttttaccaaatagaactctatca" meltingTemperature="53"><Component hybridizedRange="6787-6795" bases="tgtaaaatt"/><Component bases="a"/><Component hybridizedRange="6784-6785" bases="GC"/><Component bases="A"/><Component hybridizedRange="6756-6782" bases="gcatttttaccaaatagaactctatca"/></BindingSite></Primer><Primer recentID="20" name="KROL24 PriMnoC2FR2R" sequence="atatGGTACCtgtcgaaagtgattcttagattcagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7002-7027" boundStrand="1" annealedBases="tgtcgaaagtgattcttagattcagc" meltingTemperature="56"><Component bases="atatGGTACC"/><Component hybridizedRange="7002-7027" bases="tgtcgaaagtgattcttagattcagc"/></BindingSite><BindingSite simplified="1" location="7002-7027" boundStrand="1" annealedBases="tgtcgaaagtgattcttagattcagc" meltingTemperature="56"><Component bases="atatGGTACC"/><Component hybridizedRange="7002-7027" bases="tgtcgaaagtgattcttagattcagc"/></BindingSite></Primer></Primers>
  D<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="21:33:15">2018.12.17</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                 .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAMky.E5I+Ymd=j\f^zkGR	!ySJٝ%zRHU֢m&yg)NEf]mYUN%_m^Ii1׀ޕ4V5mu&yXI&
PaZMIHcDj	U=j4VIudNaN]<zP)*DT:my^*X^XyZVEkyaYVGځj*RZ%YҪR[Nf/mNe+d'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ        >  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  6<?xml version="1.0"?><Features recycledIDs="45,44,46,43,42" nextValidID="47"><Feature recentID="9" name="P708 PriM_unst1_F1" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6173-6197" color="#50ff50" type="standard"/><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature><Feature recentID="8" name="P707 PriM_unst1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6136-6162" color="#50ff50" type="standard"/><Q name="note"><V text="P707 PriM_unst1_R1"/></Q></Feature><Feature recentID="7" name="P702 PriM_mtip1_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6886-6910" color="#50ff50" type="standard"/><Q name="note"><V text="P702 PriM_mtip1_R2"/></Q></Feature><Feature recentID="3" name="P697 Ck_PriM_mpk1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6131" color="#50ff50" type="standard"/><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature><Feature recentID="0" name="P698 Ck_PriM_mpk1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6584-6606" color="#50ff50" type="standard"/><Q name="note"><V text="P698 Ck_PriM_mpk1R"/></Q></Feature><Feature recentID="41" name="P694 PriM_mpk_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7204-7231" color="#50ff50" type="standard"/><Q name="note"><V text="P694 PriM_mpk_R2"/></Q></Feature><Feature recentID="40" name="P693 PriM_mpk_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6194-6222" color="#50ff50" type="standard"/><Q name="note"><V text="P693 PriM_mpk_F2"/></Q></Feature><Feature recentID="39" name="P692 PriM_mpk_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6143-6169" color="#50ff50" type="standard"/><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="25" name="P444 pKL75_F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6431-6457" color="#50ff50" type="standard"/><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="24" name="P440 RT_FTL0702-3R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6391-6410" color="#50ff50" type="standard"/><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="23" name="P439 RT_FTL0702-3F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6325-6346" color="#50ff50" type="standard"/><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="22" name="P438 RT_FTL0702-2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6846-6867" color="#50ff50" type="standard"/><Q name="note"><V text="P438 RT_FTL0702-2R"/></Q></Feature><Feature recentID="21" name="P437 RT_FTL0702-2F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6741-6763" color="#50ff50" type="standard"/><Q name="note"><V text="P437 RT_FTL0702-2F"/></Q></Feature><Feature recentID="20" name="P436 RT_FTL0702-1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6206-6227" color="#50ff50" type="standard"/><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="19" name="P435 RT_FTL0702-1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6127" color="#50ff50" type="standard"/><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="18" name="P433 FTL0702cmp_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6946-6973" color="#50ff50" type="standard"/><Q name="note"><V text="P433 FTL0702cmp_R2"/></Q></Feature><Feature recentID="17" name="P428 FTL0702_VSVG_2F" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6943-6970" color="#50ff50" type="standard"/><Q name="note"><V text="P428 FTL0702_VSVG_2F"/></Q></Feature><Feature recentID="16" name="P411 FTL0702sp_2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6594-6618" color="#50ff50" type="standard"/><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="15" name="P368 FTL0702seqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6663-6691" color="#8080ff" type="standard"/><Q name="note"><V text="P368 FTL0702seqR1"/></Q></Feature><Feature recentID="14" name="P365 FTL0702cmp_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6943-6970" color="#8080ff" type="standard"/><Q name="note"><V text="P365 FTL0702cmp_R"/></Q></Feature><Feature recentID="13" name="hypothetical protein" directionality="1" translationMW="31542.95" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="199" hitsStopCodon="1"><Segment range="6108-6973" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="12" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6973" color="#00e600" type="standard"/></Feature><Feature recentID="11" name="P427 FTL0702_VSVG_1R" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6974-7003" color="#50ff50" type="standard"/><Q name="note"><V text="P427 FTL0702_VSVG_1R"/></Q></Feature><Feature recentID="10" name="PRP11" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7098-7128" color="#8080ff" type="standard"/><Q name="note"><V text="PRP11"/></Q></Feature><Feature recentID="6" name="P329 SP_D0702-R" directionality="1" type="primer_bind" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7064-7091" color="#8080ff" type="standard"/><Q name="note"><V text="P329 SP_D0702-R"/></Q></Feature><Feature recentID="5" name="RPp7" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6964-6992" color="#0080ff" type="standard"/><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="4" name="FAD binding family protein" directionality="2" translationMW="7383.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="527" hitsStopCodon="1"><Segment range="7038-7231" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="2" name="FTL_0701" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7038-7231" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-7231" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="38" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7960" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="mol_type"><V predef="genomic DNA"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="37" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7232-7236" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="36" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7666-288" color="#ffff00" type="standard"/><Q name="note"><V text="ColE origin"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="35" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="34" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="32" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="30" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7258-7274" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  U<?xml version="1.0"?><Primers recycledIDs="24,23,30,29,25,37,12" nextValidID="40"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="26" name="P821 PriM_mtip1_R3" sequence="atatGGTACCgtaactttggtattagttctgatactg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6438-6464" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6438-6464" bases="gtaactttggtattagttctgatactg"/></BindingSite><BindingSite simplified="1" location="6438-6464" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6438-6464" bases="gtaactttggtattagttctgatactg"/></BindingSite></Primer><Primer recentID="1" name="P819 PriM_mpk_R2" sequence="atatGGTACCtgtaaagccaaatatactttctcttcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6897-6923" boundStrand="1" annealedBases="tgtaaagccaaatatactttctcttcg" meltingTemperature="54"><Component bases="atatGGTACC"/><Component hybridizedRange="6897-6923" bases="tgtaaagccaaatatactttctcttcg"/></BindingSite><BindingSite simplified="1" location="6897-6923" boundStrand="1" annealedBases="tgtaaagccaaatatactttctcttcg" meltingTemperature="54"><Component bases="atatGGTACC"/><Component hybridizedRange="6897-6923" bases="tgtaaagccaaatatactttctcttcg"/></BindingSite></Primer><Primer recentID="21" name="P707 PriM_unst1_R1" sequence="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6135-6181" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6172-6181" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6163-6168" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6135-6161" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite><BindingSite simplified="1" location="6135-6181" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6172-6181" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6163-6168" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6135-6161" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite></Primer><Primer recentID="28" name="P708 PriM_unst1_F1" sequence="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6152-6196" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6152-6161" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6163-6168" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6172-6196" bases="ggtgcaagagtagttctttggaatg"/></BindingSite><BindingSite simplified="1" location="6152-6196" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6152-6161" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6163-6168" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6172-6196" bases="ggtgcaagagtagttctttggaatg"/></BindingSite></Primer><Primer recentID="27" name="P428 FTL0702_VSVG_2F" sequence="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGCttcttctttaggcaaaacattacttaag" description="0"><BindingSite location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="39" name="P427 FTL0702_VSVG_1R" sequence="GATATTGAAATGAATAGATTAGGTAAATAAactaatgactaaatttctaaaattaattag" description="0"><BindingSite location="6972-7002" boundStrand="0" annealedBases="Aactaatgactaaatttctaaaattaattag" meltingTemperature="51"><Component bases="GATATTGAAATGAATAGATTAGGTAAATA"/><Component hybridizedRange="6972-7002" bases="Aactaatgactaaatttctaaaattaattag"/></BindingSite><BindingSite simplified="1" location="6972-7002" boundStrand="0" annealedBases="Aactaatgactaaatttctaaaattaattag" meltingTemperature="51"><Component bases="GATATTGAAATGAATAGATTAGGTAAATA"/><Component hybridizedRange="6972-7002" bases="Aactaatgactaaatttctaaaattaattag"/></BindingSite></Primer><Primer recentID="38" name="P693 PriM_mpk_F2" sequence="GCAggtgcaGCAgtagttcttGCAaatggtcaatataacatcaatagcaaaga" description="0"><BindingSite location="6172-6221" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6172-6177" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6180-6189" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6193-6221" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite><BindingSite simplified="1" location="6172-6221" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6172-6177" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6180-6189" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6193-6221" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite></Primer><Primer recentID="36" name="P692 PriM_mpk_R1" sequence="TGCaagaactacTGCtgcaccTGCgaacatatcaatgttgcttctgtagtc" description="0"><BindingSite location="6142-6189" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6180-6189" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6172-6177" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6142-6168" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite><BindingSite simplified="1" location="6142-6189" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6180-6189" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6172-6177" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6142-6168" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite></Primer><Primer recentID="35" name="P365 FTL0702cmp_R1" sequence="atatGCGGCCGCttcttctttaggcaaaacattacttaag" description="Contains NotI site"><BindingSite location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="34" name="P694 PriM_mpk_R2" sequence="GGTACCaagctatgaacatgattatagctttagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7203-7236" boundStrand="1" annealedBases="GGTACCaagctatgaacatgattatagctttagc" meltingTemperature="60"><Component hybridizedRange="7203-7236" bases="GGTACCaagctatgaacatgattatagctttagc"/></BindingSite><BindingSite simplified="1" location="7203-7236" boundStrand="1" annealedBases="GGTACCaagctatgaacatgattatagctttagc" meltingTemperature="60"><Component hybridizedRange="7203-7236" bases="GGTACCaagctatgaacatgattatagctttagc"/></BindingSite></Primer><Primer recentID="33" name="P433 FTL0702cmp_R2" sequence="atatGGATCCtcattcttctttaggcaaaacattactt" description="Contains BamHI site"><BindingSite location="6945-6972" boundStrand="1" annealedBases="tcattcttctttaggcaaaacattactt" meltingTemperature="55"><Component bases="atatGGATCC"/><Component hybridizedRange="6945-6972" bases="tcattcttctttaggcaaaacattactt"/></BindingSite><BindingSite simplified="1" location="6945-6972" boundStrand="1" annealedBases="tcattcttctttaggcaaaacattactt" meltingTemperature="55"><Component bases="atatGGATCC"/><Component hybridizedRange="6945-6972" bases="tcattcttctttaggcaaaacattactt"/></BindingSite></Primer><Primer recentID="32" name="P366 FTL0702cmp_R2" sequence="atatGGATCCttcttctttaggcaaaacattacttaag" description="&quot;Contains BamHI site, could use to make vector lacking VSVG tag&quot;"><BindingSite location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGGATCC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGGATCC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="2" name="P444 pKL75_F" sequence="atatGGTACCagcttaatcagtatcagaactaatacc" description="Contains KpnI site"><BindingSite location="6430-6456" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6430-6456" bases="agcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6430-6456" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6430-6456" bases="agcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="8" name="P695 PriM_mpk_R3" sequence="catcagcTGCtagtgaaactgctttagttgtatcact" description="0"><BindingSite location="6295-6331" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6325-6331" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6295-6321" bases="tagtgaaactgctttagttgtatcact"/></BindingSite><BindingSite simplified="1" location="6295-6331" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6325-6331" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6295-6321" bases="tagtgaaactgctttagttgtatcact"/></BindingSite></Primer><Primer recentID="31" name="P696 PriM_mpk_F4" sequence="ttcactaGCAgctgatgctaacacacttacatatac" description="0"><BindingSite location="6325-6350" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6325-6350" bases="gctgatgctaacacacttacatatac"/></BindingSite><BindingSite simplified="1" location="6325-6350" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6325-6350" bases="gctgatgctaacacacttacatatac"/></BindingSite></Primer><Primer recentID="22" name="P702 PriM_mtip1_R2" sequence="atatGGTACCtactttctcttcgaaaacggtgtcg" description="Contains KpnI site"><BindingSite location="6885-6909" boundStrand="1" annealedBases="tactttctcttcgaaaacggtgtcg" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6885-6909" bases="tactttctcttcgaaaacggtgtcg"/></BindingSite><BindingSite simplified="1" location="6885-6909" boundStrand="1" annealedBases="tactttctcttcgaaaacggtgtcg" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6885-6909" bases="tactttctcttcgaaaacggtgtcg"/></BindingSite></Primer><Primer recentID="0" name="P411 FTL0702sp_2R" sequence="atatGGTACCgcatttgtagctgctttaagtgccc" description="Contains KpnI site"><BindingSite location="6593-6617" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="6593-6617" bases="gcatttgtagctgctttaagtgccc"/></BindingSite><BindingSite simplified="1" location="6593-6617" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="6593-6617" bases="gcatttgtagctgctttaagtgccc"/></BindingSite></Primer><Primer recentID="3" name="P368 FTL0702seqR1" sequence="acttgtagatattaacattaaagtatgac" description="0"><BindingSite location="6662-6690" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="acttgtagatattaacattaaagtatgac"/></BindingSite><BindingSite simplified="1" location="6662-6690" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="acttgtagatattaacattaaagtatgac"/></BindingSite></Primer><Primer recentID="4" name="P430 CkFTL0702V_R" sequence="gtcatactttaatgttaatatctacaagt" description="0"><BindingSite location="6662-6690" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite><BindingSite simplified="1" location="6662-6690" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite></Primer><Primer recentID="5" name="P329 SP_D0702-R" sequence="aatgatgacaataaaatagaccatttgg" description="0"><BindingSite location="7063-7090" boundStrand="0" annealedBases="aatgatgacaataaaatagaccatttgg" meltingTemperature="55"><Component hybridizedRange="7063-7090" bases="aatgatgacaataaaatagaccatttgg"/></BindingSite><BindingSite simplified="1" location="7063-7090" boundStrand="0" annealedBases="aatgatgacaataaaatagaccatttgg" meltingTemperature="55"><Component hybridizedRange="7063-7090" bases="aatgatgacaataaaatagaccatttgg"/></BindingSite></Primer><Primer recentID="6" name="P697 Ck_PriM_mpk1F" sequence="cagcatgggattctgctactagtc" description="0"><BindingSite location="6107-6130" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="58"><Component hybridizedRange="6107-6130" bases="cagcatgggattctgctactagtc"/></BindingSite><BindingSite simplified="1" location="6107-6130" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="58"><Component hybridizedRange="6107-6130" bases="cagcatgggattctgctactagtc"/></BindingSite></Primer><Primer recentID="7" name="P437 RT_FTL0702-2F" sequence="agcaagtaagtgatgctgataga" description="0"><BindingSite location="6740-6762" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="6740-6762" bases="agcaagtaagtgatgctgataga"/></BindingSite><BindingSite simplified="1" location="6740-6762" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="6740-6762" bases="agcaagtaagtgatgctgataga"/></BindingSite></Primer><Primer recentID="9" name="P698 Ck_PriM_mpk1R" sequence="gctttaagtgcccagttgtcgcc" description="0"><BindingSite location="6583-6605" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="6583-6605" bases="gctttaagtgcccagttgtcgcc"/></BindingSite><BindingSite simplified="1" location="6583-6605" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="6583-6605" bases="gctttaagtgcccagttgtcgcc"/></BindingSite></Primer><Primer recentID="10" name="P438 RT_FTL0702-2R" sequence="agttgtccggctatctgtttag" description="0"><BindingSite location="6845-6866" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="6845-6866" bases="agttgtccggctatctgtttag"/></BindingSite><BindingSite simplified="1" location="6845-6866" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="6845-6866" bases="agttgtccggctatctgtttag"/></BindingSite></Primer><Primer recentID="11" name="P439 RT_FTL0702-3F" sequence="tgctgatgctaacacacttaca" description="0"><BindingSite location="6324-6345" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6324-6345" bases="tgctgatgctaacacacttaca"/></BindingSite><BindingSite simplified="1" location="6324-6345" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6324-6345" bases="tgctgatgctaacacacttaca"/></BindingSite></Primer><Primer recentID="13" name="P436 RT_FTL0702-1R" sequence="cggcatctttgctattgatgtt" description="0"><BindingSite location="6205-6226" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6205-6226" bases="cggcatctttgctattgatgtt"/></BindingSite><BindingSite simplified="1" location="6205-6226" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6205-6226" bases="cggcatctttgctattgatgtt"/></BindingSite></Primer><Primer recentID="14" name="P440 RT_FTL0702-3R" sequence="ccgttcttggcgaagctatt" description="0"><BindingSite location="6390-6409" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6390-6409" bases="ccgttcttggcgaagctatt"/></BindingSite><BindingSite simplified="1" location="6390-6409" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6390-6409" bases="ccgttcttggcgaagctatt"/></BindingSite></Primer><Primer recentID="15" name="P435 RT_FTL0702-1F" sequence="cagcatgggattctgctact" description="0"><BindingSite location="6107-6126" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6107-6126" bases="cagcatgggattctgctact"/></BindingSite><BindingSite simplified="1" location="6107-6126" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6107-6126" bases="cagcatgggattctgctact"/></BindingSite></Primer><Primer recentID="16" name="KROL20 PriMnoC1FR1R" sequence="aattgatagagccgttcttggcgaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6396-6420" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6396-6420" bases="aattgatagagccgttcttggcgaa"/></BindingSite><BindingSite simplified="1" location="6396-6420" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6396-6420" bases="aattgatagagccgttcttggcgaa"/></BindingSite></Primer><Primer recentID="17" name="KROL21 PriMnoC1FR2F" sequence="atatGCtgaagctagcttaatcagtatcagaactaatacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6423-6456" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="58"><Component bases="atatGC"/><Component hybridizedRange="6423-6456" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6423-6456" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="58"><Component bases="atatGC"/><Component hybridizedRange="6423-6456" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="18" name="KROL23 PriMnoC2FR2F" sequence="aaaaatgcaGCAaattttacagcagctaaaaatagtaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6775-6814" boundStrand="0" annealedBases="aaaaatgcaGCaattttacagcagctaaaaatagtaaca" meltingTemperature="59"><Component hybridizedRange="6775-6785" bases="aaaaatgcaGC"/><Component bases="A"/><Component hybridizedRange="6787-6814" bases="aattttacagcagctaaaaatagtaaca"/></BindingSite><BindingSite simplified="1" location="6775-6814" boundStrand="0" annealedBases="aaaaatgcaGCaattttacagcagctaaaaatagtaaca" meltingTemperature="59"><Component hybridizedRange="6775-6785" bases="aaaaatgcaGC"/><Component bases="A"/><Component hybridizedRange="6787-6814" bases="aattttacagcagctaaaaatagtaaca"/></BindingSite></Primer><Primer recentID="19" name="KROL22 PriMnoC2FR1R" sequence="tgtaaaattaGCAgcatttttaccaaatagaactctatca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6756-6795" boundStrand="1" annealedBases="tgtaaaattGCgcatttttaccaaatagaactctatca" meltingTemperature="53"><Component hybridizedRange="6787-6795" bases="tgtaaaatt"/><Component bases="a"/><Component hybridizedRange="6784-6785" bases="GC"/><Component bases="A"/><Component hybridizedRange="6756-6782" bases="gcatttttaccaaatagaactctatca"/></BindingSite><BindingSite simplified="1" location="6756-6795" boundStrand="1" annealedBases="tgtaaaattGCgcatttttaccaaatagaactctatca" meltingTemperature="53"><Component hybridizedRange="6787-6795" bases="tgtaaaatt"/><Component bases="a"/><Component hybridizedRange="6784-6785" bases="GC"/><Component bases="A"/><Component hybridizedRange="6756-6782" bases="gcatttttaccaaatagaactctatca"/></BindingSite></Primer><Primer recentID="20" name="KROL24 PriMnoC2FR2R" sequence="atatGGTACCtgtcgaaagtgattcttagattcagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7002-7027" boundStrand="1" annealedBases="tgtcgaaagtgattcttagattcagc" meltingTemperature="56"><Component bases="atatGGTACC"/><Component hybridizedRange="7002-7027" bases="tgtcgaaagtgattcttagattcagc"/></BindingSite><BindingSite simplified="1" location="7002-7027" boundStrand="1" annealedBases="tgtcgaaagtgattcttagattcagc" meltingTemperature="56"><Component bases="atatGGTACC"/><Component hybridizedRange="7002-7027" bases="tgtcgaaagtgattcttagattcagc"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="21:33:6">2018.12.17</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAMky.E5I+Ymd=j\f^zkGR	!ySJٝ%zRHU֢m&yg)NEf]mYUN%_m^Ii1׀ޕ4V5mu&yXI&
PaZMIHcDj	U5j4VIudNaN]<zP)*DT:my^*X^XyZVEkyaYVGځj*RZ%YҪR[Nf/mNe+d'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ        >  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  6<?xml version="1.0"?><Features recycledIDs="45,44,46,43,42" nextValidID="47"><Feature recentID="9" name="P708 PriM_unst1_F1" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6173-6197" color="#50ff50" type="standard"/><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature><Feature recentID="8" name="P707 PriM_unst1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6136-6162" color="#50ff50" type="standard"/><Q name="note"><V text="P707 PriM_unst1_R1"/></Q></Feature><Feature recentID="7" name="P702 PriM_mtip1_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6886-6910" color="#50ff50" type="standard"/><Q name="note"><V text="P702 PriM_mtip1_R2"/></Q></Feature><Feature recentID="3" name="P697 Ck_PriM_mpk1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6131" color="#50ff50" type="standard"/><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature><Feature recentID="0" name="P698 Ck_PriM_mpk1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6584-6606" color="#50ff50" type="standard"/><Q name="note"><V text="P698 Ck_PriM_mpk1R"/></Q></Feature><Feature recentID="41" name="P694 PriM_mpk_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7204-7231" color="#50ff50" type="standard"/><Q name="note"><V text="P694 PriM_mpk_R2"/></Q></Feature><Feature recentID="40" name="P693 PriM_mpk_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6194-6222" color="#50ff50" type="standard"/><Q name="note"><V text="P693 PriM_mpk_F2"/></Q></Feature><Feature recentID="39" name="P692 PriM_mpk_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6143-6169" color="#50ff50" type="standard"/><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="25" name="P444 pKL75_F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6431-6457" color="#50ff50" type="standard"/><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="24" name="P440 RT_FTL0702-3R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6391-6410" color="#50ff50" type="standard"/><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="23" name="P439 RT_FTL0702-3F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6325-6346" color="#50ff50" type="standard"/><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="22" name="P438 RT_FTL0702-2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6846-6867" color="#50ff50" type="standard"/><Q name="note"><V text="P438 RT_FTL0702-2R"/></Q></Feature><Feature recentID="21" name="P437 RT_FTL0702-2F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6741-6763" color="#50ff50" type="standard"/><Q name="note"><V text="P437 RT_FTL0702-2F"/></Q></Feature><Feature recentID="20" name="P436 RT_FTL0702-1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6206-6227" color="#50ff50" type="standard"/><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="19" name="P435 RT_FTL0702-1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6127" color="#50ff50" type="standard"/><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="18" name="P433 FTL0702cmp_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6946-6973" color="#50ff50" type="standard"/><Q name="note"><V text="P433 FTL0702cmp_R2"/></Q></Feature><Feature recentID="17" name="P428 FTL0702_VSVG_2F" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6943-6970" color="#50ff50" type="standard"/><Q name="note"><V text="P428 FTL0702_VSVG_2F"/></Q></Feature><Feature recentID="16" name="P411 FTL0702sp_2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6594-6618" color="#50ff50" type="standard"/><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="15" name="P368 FTL0702seqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6663-6691" color="#8080ff" type="standard"/><Q name="note"><V text="P368 FTL0702seqR1"/></Q></Feature><Feature recentID="14" name="P365 FTL0702cmp_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6943-6970" color="#8080ff" type="standard"/><Q name="note"><V text="P365 FTL0702cmp_R"/></Q></Feature><Feature recentID="13" name="hypothetical protein" directionality="1" translationMW="31542.95" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="199" hitsStopCodon="1"><Segment range="6108-6973" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="12" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6973" color="#00e600" type="standard"/></Feature><Feature recentID="11" name="P427 FTL0702_VSVG_1R" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6974-7003" color="#50ff50" type="standard"/><Q name="note"><V text="P427 FTL0702_VSVG_1R"/></Q></Feature><Feature recentID="10" name="PRP11" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7098-7128" color="#8080ff" type="standard"/><Q name="note"><V text="PRP11"/></Q></Feature><Feature recentID="6" name="P329 SP_D0702-R" directionality="1" type="primer_bind" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7064-7091" color="#8080ff" type="standard"/><Q name="note"><V text="P329 SP_D0702-R"/></Q></Feature><Feature recentID="5" name="RPp7" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6964-6992" color="#0080ff" type="standard"/><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="4" name="FAD binding family protein" directionality="2" translationMW="7383.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="527" hitsStopCodon="1"><Segment range="7038-7231" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="2" name="FTL_0701" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7038-7231" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-7231" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="38" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7960" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="mol_type"><V predef="genomic DNA"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="37" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7232-7236" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="36" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7666-288" color="#ffff00" type="standard"/><Q name="note"><V text="ColE origin"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="35" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="34" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="32" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="30" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7258-7274" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  Ut<?xml version="1.0"?><Primers recycledIDs="24,23,30,29,25,37,12" nextValidID="40"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="26" name="P821 PriM_mtip1_R3" sequence="atatGGTACCgtaactttggtattagttctgatactg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6438-6464" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6438-6464" bases="gtaactttggtattagttctgatactg"/></BindingSite><BindingSite simplified="1" location="6438-6464" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6438-6464" bases="gtaactttggtattagttctgatactg"/></BindingSite></Primer><Primer recentID="1" name="P819 PriM_mpk_R2" sequence="atatGGTACCtgtaaagccaaatatactttctcttcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6897-6923" boundStrand="1" annealedBases="tgtaaagccaaatatactttctcttcg" meltingTemperature="54"><Component bases="atatGGTACC"/><Component hybridizedRange="6897-6923" bases="tgtaaagccaaatatactttctcttcg"/></BindingSite><BindingSite simplified="1" location="6897-6923" boundStrand="1" annealedBases="tgtaaagccaaatatactttctcttcg" meltingTemperature="54"><Component bases="atatGGTACC"/><Component hybridizedRange="6897-6923" bases="tgtaaagccaaatatactttctcttcg"/></BindingSite></Primer><Primer recentID="21" name="P707 PriM_unst1_R1" sequence="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6135-6181" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6172-6181" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6163-6168" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6135-6161" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite><BindingSite simplified="1" location="6135-6181" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6172-6181" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6163-6168" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6135-6161" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite></Primer><Primer recentID="28" name="P708 PriM_unst1_F1" sequence="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6152-6196" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6152-6161" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6163-6168" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6172-6196" bases="ggtgcaagagtagttctttggaatg"/></BindingSite><BindingSite simplified="1" location="6152-6196" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6152-6161" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6163-6168" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6172-6196" bases="ggtgcaagagtagttctttggaatg"/></BindingSite></Primer><Primer recentID="27" name="P428 FTL0702_VSVG_2F" sequence="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGCttcttctttaggcaaaacattacttaag" description="0"><BindingSite location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="39" name="P427 FTL0702_VSVG_1R" sequence="GATATTGAAATGAATAGATTAGGTAAATAAactaatgactaaatttctaaaattaattag" description="0"><BindingSite location="6972-7002" boundStrand="0" annealedBases="Aactaatgactaaatttctaaaattaattag" meltingTemperature="51"><Component bases="GATATTGAAATGAATAGATTAGGTAAATA"/><Component hybridizedRange="6972-7002" bases="Aactaatgactaaatttctaaaattaattag"/></BindingSite><BindingSite simplified="1" location="6972-7002" boundStrand="0" annealedBases="Aactaatgactaaatttctaaaattaattag" meltingTemperature="51"><Component bases="GATATTGAAATGAATAGATTAGGTAAATA"/><Component hybridizedRange="6972-7002" bases="Aactaatgactaaatttctaaaattaattag"/></BindingSite></Primer><Primer recentID="38" name="P693 PriM_mpk_F2" sequence="GCAggtgcaGCAgtagttcttGCAaatggtcaatataacatcaatagcaaaga" description="0"><BindingSite location="6172-6221" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6172-6177" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6180-6189" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6193-6221" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite><BindingSite simplified="1" location="6172-6221" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6172-6177" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6180-6189" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6193-6221" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite></Primer><Primer recentID="36" name="P692 PriM_mpk_R1" sequence="TGCaagaactacTGCtgcaccTGCgaacatatcaatgttgcttctgtagtc" description="0"><BindingSite location="6142-6189" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6180-6189" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6172-6177" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6142-6168" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite><BindingSite simplified="1" location="6142-6189" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6180-6189" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6172-6177" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6142-6168" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite></Primer><Primer recentID="35" name="P365 FTL0702cmp_R1" sequence="atatGCGGCCGCttcttctttaggcaaaacattacttaag" description="Contains NotI site"><BindingSite location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="34" name="P694 PriM_mpk_R2" sequence="GGTACCaagctatgaacatgattatagctttagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7203-7236" boundStrand="1" annealedBases="GGTACCaagctatgaacatgattatagctttagc" meltingTemperature="60"><Component hybridizedRange="7203-7236" bases="GGTACCaagctatgaacatgattatagctttagc"/></BindingSite><BindingSite simplified="1" location="7203-7236" boundStrand="1" annealedBases="GGTACCaagctatgaacatgattatagctttagc" meltingTemperature="60"><Component hybridizedRange="7203-7236" bases="GGTACCaagctatgaacatgattatagctttagc"/></BindingSite></Primer><Primer recentID="33" name="P433 FTL0702cmp_R2" sequence="atatGGATCCtcattcttctttaggcaaaacattactt" description="Contains BamHI site"><BindingSite location="6945-6972" boundStrand="1" annealedBases="tcattcttctttaggcaaaacattactt" meltingTemperature="55"><Component bases="atatGGATCC"/><Component hybridizedRange="6945-6972" bases="tcattcttctttaggcaaaacattactt"/></BindingSite><BindingSite simplified="1" location="6945-6972" boundStrand="1" annealedBases="tcattcttctttaggcaaaacattactt" meltingTemperature="55"><Component bases="atatGGATCC"/><Component hybridizedRange="6945-6972" bases="tcattcttctttaggcaaaacattactt"/></BindingSite></Primer><Primer recentID="32" name="P366 FTL0702cmp_R2" sequence="atatGGATCCttcttctttaggcaaaacattacttaag" description="&quot;Contains BamHI site, could use to make vector lacking VSVG tag&quot;"><BindingSite location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGGATCC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGGATCC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="2" name="P444 pKL75_F" sequence="atatGGTACCagcttaatcagtatcagaactaatacc" description="Contains KpnI site"><BindingSite location="6430-6456" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6430-6456" bases="agcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6430-6456" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6430-6456" bases="agcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="8" name="P695 PriM_mpk_R3" sequence="catcagcTGCtagtgaaactgctttagttgtatcact" description="0"><BindingSite location="6295-6331" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6325-6331" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6295-6321" bases="tagtgaaactgctttagttgtatcact"/></BindingSite><BindingSite simplified="1" location="6295-6331" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6325-6331" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6295-6321" bases="tagtgaaactgctttagttgtatcact"/></BindingSite></Primer><Primer recentID="31" name="P696 PriM_mpk_F4" sequence="ttcactaGCAgctgatgctaacacacttacatatac" description="0"><BindingSite location="6325-6350" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6325-6350" bases="gctgatgctaacacacttacatatac"/></BindingSite><BindingSite simplified="1" location="6325-6350" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6325-6350" bases="gctgatgctaacacacttacatatac"/></BindingSite></Primer><Primer recentID="22" name="P702 PriM_mtip1_R2" sequence="atatGGTACCtactttctcttcgaaaacggtgtcg" description="Contains KpnI site"><BindingSite location="6885-6909" boundStrand="1" annealedBases="tactttctcttcgaaaacggtgtcg" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6885-6909" bases="tactttctcttcgaaaacggtgtcg"/></BindingSite><BindingSite simplified="1" location="6885-6909" boundStrand="1" annealedBases="tactttctcttcgaaaacggtgtcg" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6885-6909" bases="tactttctcttcgaaaacggtgtcg"/></BindingSite></Primer><Primer recentID="0" name="P411 FTL0702sp_2R" sequence="atatGGTACCgcatttgtagctgctttaagtgccc" description="Contains KpnI site"><BindingSite location="6593-6617" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="6593-6617" bases="gcatttgtagctgctttaagtgccc"/></BindingSite><BindingSite simplified="1" location="6593-6617" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="6593-6617" bases="gcatttgtagctgctttaagtgccc"/></BindingSite></Primer><Primer recentID="3" name="P368 FTL0702seqR1" sequence="acttgtagatattaacattaaagtatgac" description="0"><BindingSite location="6662-6690" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="acttgtagatattaacattaaagtatgac"/></BindingSite><BindingSite simplified="1" location="6662-6690" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="acttgtagatattaacattaaagtatgac"/></BindingSite></Primer><Primer recentID="4" name="P430 CkFTL0702V_R" sequence="gtcatactttaatgttaatatctacaagt" description="0"><BindingSite location="6662-6690" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite><BindingSite simplified="1" location="6662-6690" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite></Primer><Primer recentID="5" name="P329 SP_D0702-R" sequence="aatgatgacaataaaatagaccatttgg" description="0"><BindingSite location="7063-7090" boundStrand="0" annealedBases="aatgatgacaataaaatagaccatttgg" meltingTemperature="55"><Component hybridizedRange="7063-7090" bases="aatgatgacaataaaatagaccatttgg"/></BindingSite><BindingSite simplified="1" location="7063-7090" boundStrand="0" annealedBases="aatgatgacaataaaatagaccatttgg" meltingTemperature="55"><Component hybridizedRange="7063-7090" bases="aatgatgacaataaaatagaccatttgg"/></BindingSite></Primer><Primer recentID="6" name="P697 Ck_PriM_mpk1F" sequence="cagcatgggattctgctactagtc" description="0"><BindingSite location="6107-6130" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="58"><Component hybridizedRange="6107-6130" bases="cagcatgggattctgctactagtc"/></BindingSite><BindingSite simplified="1" location="6107-6130" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="58"><Component hybridizedRange="6107-6130" bases="cagcatgggattctgctactagtc"/></BindingSite></Primer><Primer recentID="7" name="P437 RT_FTL0702-2F" sequence="agcaagtaagtgatgctgataga" description="0"><BindingSite location="6740-6762" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="6740-6762" bases="agcaagtaagtgatgctgataga"/></BindingSite><BindingSite simplified="1" location="6740-6762" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="6740-6762" bases="agcaagtaagtgatgctgataga"/></BindingSite></Primer><Primer recentID="9" name="P698 Ck_PriM_mpk1R" sequence="gctttaagtgcccagttgtcgcc" description="0"><BindingSite location="6583-6605" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="6583-6605" bases="gctttaagtgcccagttgtcgcc"/></BindingSite><BindingSite simplified="1" location="6583-6605" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="6583-6605" bases="gctttaagtgcccagttgtcgcc"/></BindingSite></Primer><Primer recentID="10" name="P438 RT_FTL0702-2R" sequence="agttgtccggctatctgtttag" description="0"><BindingSite location="6845-6866" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="6845-6866" bases="agttgtccggctatctgtttag"/></BindingSite><BindingSite simplified="1" location="6845-6866" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="6845-6866" bases="agttgtccggctatctgtttag"/></BindingSite></Primer><Primer recentID="11" name="P439 RT_FTL0702-3F" sequence="tgctgatgctaacacacttaca" description="0"><BindingSite location="6324-6345" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6324-6345" bases="tgctgatgctaacacacttaca"/></BindingSite><BindingSite simplified="1" location="6324-6345" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6324-6345" bases="tgctgatgctaacacacttaca"/></BindingSite></Primer><Primer recentID="13" name="P436 RT_FTL0702-1R" sequence="cggcatctttgctattgatgtt" description="0"><BindingSite location="6205-6226" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6205-6226" bases="cggcatctttgctattgatgtt"/></BindingSite><BindingSite simplified="1" location="6205-6226" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6205-6226" bases="cggcatctttgctattgatgtt"/></BindingSite></Primer><Primer recentID="14" name="P440 RT_FTL0702-3R" sequence="ccgttcttggcgaagctatt" description="0"><BindingSite location="6390-6409" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6390-6409" bases="ccgttcttggcgaagctatt"/></BindingSite><BindingSite simplified="1" location="6390-6409" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6390-6409" bases="ccgttcttggcgaagctatt"/></BindingSite></Primer><Primer recentID="15" name="P435 RT_FTL0702-1F" sequence="cagcatgggattctgctact" description="0"><BindingSite location="6107-6126" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6107-6126" bases="cagcatgggattctgctact"/></BindingSite><BindingSite simplified="1" location="6107-6126" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6107-6126" bases="cagcatgggattctgctact"/></BindingSite></Primer><Primer recentID="16" name="KROL20 PriMnoC1FR1R" sequence="aattgatagagccgttcttggcgaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6396-6420" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6396-6420" bases="aattgatagagccgttcttggcgaa"/></BindingSite><BindingSite simplified="1" location="6396-6420" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6396-6420" bases="aattgatagagccgttcttggcgaa"/></BindingSite></Primer><Primer recentID="17" name="KROL21 PriMnoC1FR2F" sequence="atatGCtgaagctagcttaatcagtatcagaactaatacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6423-6456" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="58"><Component bases="atatGC"/><Component hybridizedRange="6423-6456" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6423-6456" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="58"><Component bases="atatGC"/><Component hybridizedRange="6423-6456" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="18" name="KROL23 PriMnoC2FR2F" sequence="aaaaatgcaGCAaattttacagcagctaaaaatagtaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6775-6814" boundStrand="0" annealedBases="aaaaatgcaGCAaattttacagcagctaaaaatagtaaca" meltingTemperature="62"><Component hybridizedRange="6775-6814" bases="aaaaatgcaGCAaattttacagcagctaaaaatagtaaca"/></BindingSite><BindingSite simplified="1" location="6775-6814" boundStrand="0" annealedBases="aaaaatgcaGCAaattttacagcagctaaaaatagtaaca" meltingTemperature="62"><Component hybridizedRange="6775-6814" bases="aaaaatgcaGCAaattttacagcagctaaaaatagtaaca"/></BindingSite></Primer><Primer recentID="19" name="KROL22 PriMnoC2FR1R" sequence="tgtaaaattaGCAgcatttttaccaaatagaactctatca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6756-6795" boundStrand="1" annealedBases="tgtaaaattGCgcatttttaccaaatagaactctatca" meltingTemperature="53"><Component hybridizedRange="6787-6795" bases="tgtaaaatt"/><Component bases="a"/><Component hybridizedRange="6784-6785" bases="GC"/><Component bases="A"/><Component hybridizedRange="6756-6782" bases="gcatttttaccaaatagaactctatca"/></BindingSite><BindingSite simplified="1" location="6756-6795" boundStrand="1" annealedBases="tgtaaaattGCgcatttttaccaaatagaactctatca" meltingTemperature="53"><Component hybridizedRange="6787-6795" bases="tgtaaaatt"/><Component bases="a"/><Component hybridizedRange="6784-6785" bases="GC"/><Component bases="A"/><Component hybridizedRange="6756-6782" bases="gcatttttaccaaatagaactctatca"/></BindingSite></Primer><Primer recentID="20" name="KROL24 PriMnoC2FR2R" sequence="atatGGTACCtgtcgaaagtgattcttagattcagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7002-7027" boundStrand="1" annealedBases="tgtcgaaagtgattcttagattcagc" meltingTemperature="56"><Component bases="atatGGTACC"/><Component hybridizedRange="7002-7027" bases="tgtcgaaagtgattcttagattcagc"/></BindingSite><BindingSite simplified="1" location="7002-7027" boundStrand="1" annealedBases="tgtcgaaagtgattcttagattcagc" meltingTemperature="56"><Component bases="atatGGTACC"/><Component hybridizedRange="7002-7027" bases="tgtcgaaagtgattcttagattcagc"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="21:5:4">2018.12.17</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAMky.E5I+Ymd=j\f^zkGR	!ySJٝ%zRHU֢m&yg)NEf]mYUN%_m^Ii1׀ޕ4V5mu&yXI&
PaZMIHcDj	Uj4VIudNaN]<zP)*DT:my^*X^XyZVEkyaYVGځj*RZ%YҪR[Nf/mNe+d'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ    >  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  6<?xml version="1.0"?><Features recycledIDs="45,44,46,43,42" nextValidID="47"><Feature recentID="9" name="P708 PriM_unst1_F1" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6173-6197" color="#50ff50" type="standard"/><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature><Feature recentID="8" name="P707 PriM_unst1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6136-6162" color="#50ff50" type="standard"/><Q name="note"><V text="P707 PriM_unst1_R1"/></Q></Feature><Feature recentID="7" name="P702 PriM_mtip1_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6886-6910" color="#50ff50" type="standard"/><Q name="note"><V text="P702 PriM_mtip1_R2"/></Q></Feature><Feature recentID="3" name="P697 Ck_PriM_mpk1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6131" color="#50ff50" type="standard"/><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature><Feature recentID="0" name="P698 Ck_PriM_mpk1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6584-6606" color="#50ff50" type="standard"/><Q name="note"><V text="P698 Ck_PriM_mpk1R"/></Q></Feature><Feature recentID="41" name="P694 PriM_mpk_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7204-7231" color="#50ff50" type="standard"/><Q name="note"><V text="P694 PriM_mpk_R2"/></Q></Feature><Feature recentID="40" name="P693 PriM_mpk_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6194-6222" color="#50ff50" type="standard"/><Q name="note"><V text="P693 PriM_mpk_F2"/></Q></Feature><Feature recentID="39" name="P692 PriM_mpk_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6143-6169" color="#50ff50" type="standard"/><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="25" name="P444 pKL75_F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6431-6457" color="#50ff50" type="standard"/><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="24" name="P440 RT_FTL0702-3R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6391-6410" color="#50ff50" type="standard"/><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="23" name="P439 RT_FTL0702-3F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6325-6346" color="#50ff50" type="standard"/><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="22" name="P438 RT_FTL0702-2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6846-6867" color="#50ff50" type="standard"/><Q name="note"><V text="P438 RT_FTL0702-2R"/></Q></Feature><Feature recentID="21" name="P437 RT_FTL0702-2F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6741-6763" color="#50ff50" type="standard"/><Q name="note"><V text="P437 RT_FTL0702-2F"/></Q></Feature><Feature recentID="20" name="P436 RT_FTL0702-1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6206-6227" color="#50ff50" type="standard"/><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="19" name="P435 RT_FTL0702-1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6127" color="#50ff50" type="standard"/><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="18" name="P433 FTL0702cmp_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6946-6973" color="#50ff50" type="standard"/><Q name="note"><V text="P433 FTL0702cmp_R2"/></Q></Feature><Feature recentID="17" name="P428 FTL0702_VSVG_2F" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6943-6970" color="#50ff50" type="standard"/><Q name="note"><V text="P428 FTL0702_VSVG_2F"/></Q></Feature><Feature recentID="16" name="P411 FTL0702sp_2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6594-6618" color="#50ff50" type="standard"/><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="15" name="P368 FTL0702seqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6663-6691" color="#8080ff" type="standard"/><Q name="note"><V text="P368 FTL0702seqR1"/></Q></Feature><Feature recentID="14" name="P365 FTL0702cmp_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6943-6970" color="#8080ff" type="standard"/><Q name="note"><V text="P365 FTL0702cmp_R"/></Q></Feature><Feature recentID="13" name="hypothetical protein" directionality="1" translationMW="31575.01" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="199" hitsStopCodon="1"><Segment range="6108-6973" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="12" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6973" color="#00e600" type="standard"/></Feature><Feature recentID="11" name="P427 FTL0702_VSVG_1R" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6974-7003" color="#50ff50" type="standard"/><Q name="note"><V text="P427 FTL0702_VSVG_1R"/></Q></Feature><Feature recentID="10" name="PRP11" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7098-7128" color="#8080ff" type="standard"/><Q name="note"><V text="PRP11"/></Q></Feature><Feature recentID="6" name="P329 SP_D0702-R" directionality="1" type="primer_bind" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7064-7091" color="#8080ff" type="standard"/><Q name="note"><V text="P329 SP_D0702-R"/></Q></Feature><Feature recentID="5" name="RPp7" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6964-6992" color="#0080ff" type="standard"/><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="4" name="FAD binding family protein" directionality="2" translationMW="7383.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="527" hitsStopCodon="1"><Segment range="7038-7231" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="2" name="FTL_0701" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7038-7231" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-7231" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="38" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7960" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="mol_type"><V predef="genomic DNA"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="37" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7232-7236" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="36" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7666-288" color="#ffff00" type="standard"/><Q name="note"><V text="ColE origin"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="35" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="34" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="32" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="30" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7258-7274" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  Ul<?xml version="1.0"?><Primers recycledIDs="24,23,30,29,25,37,12" nextValidID="40"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="26" name="P821 PriM_mtip1_R3" sequence="atatGGTACCgtaactttggtattagttctgatactg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6438-6464" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6438-6464" bases="gtaactttggtattagttctgatactg"/></BindingSite><BindingSite simplified="1" location="6438-6464" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6438-6464" bases="gtaactttggtattagttctgatactg"/></BindingSite></Primer><Primer recentID="1" name="P819 PriM_mpk_R2" sequence="atatGGTACCtgtaaagccaaatatactttctcttcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6897-6923" boundStrand="1" annealedBases="tgtaaagccaaatatactttctcttcg" meltingTemperature="54"><Component bases="atatGGTACC"/><Component hybridizedRange="6897-6923" bases="tgtaaagccaaatatactttctcttcg"/></BindingSite><BindingSite simplified="1" location="6897-6923" boundStrand="1" annealedBases="tgtaaagccaaatatactttctcttcg" meltingTemperature="54"><Component bases="atatGGTACC"/><Component hybridizedRange="6897-6923" bases="tgtaaagccaaatatactttctcttcg"/></BindingSite></Primer><Primer recentID="21" name="P707 PriM_unst1_R1" sequence="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6135-6181" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6172-6181" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6163-6168" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6135-6161" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite><BindingSite simplified="1" location="6135-6181" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6172-6181" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6163-6168" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6135-6161" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite></Primer><Primer recentID="28" name="P708 PriM_unst1_F1" sequence="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6152-6196" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6152-6161" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6163-6168" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6172-6196" bases="ggtgcaagagtagttctttggaatg"/></BindingSite><BindingSite simplified="1" location="6152-6196" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6152-6161" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6163-6168" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6172-6196" bases="ggtgcaagagtagttctttggaatg"/></BindingSite></Primer><Primer recentID="27" name="P428 FTL0702_VSVG_2F" sequence="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGCttcttctttaggcaaaacattacttaag" description="0"><BindingSite location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="39" name="P427 FTL0702_VSVG_1R" sequence="GATATTGAAATGAATAGATTAGGTAAATAAactaatgactaaatttctaaaattaattag" description="0"><BindingSite location="6972-7002" boundStrand="0" annealedBases="Aactaatgactaaatttctaaaattaattag" meltingTemperature="51"><Component bases="GATATTGAAATGAATAGATTAGGTAAATA"/><Component hybridizedRange="6972-7002" bases="Aactaatgactaaatttctaaaattaattag"/></BindingSite><BindingSite simplified="1" location="6972-7002" boundStrand="0" annealedBases="Aactaatgactaaatttctaaaattaattag" meltingTemperature="51"><Component bases="GATATTGAAATGAATAGATTAGGTAAATA"/><Component hybridizedRange="6972-7002" bases="Aactaatgactaaatttctaaaattaattag"/></BindingSite></Primer><Primer recentID="38" name="P693 PriM_mpk_F2" sequence="GCAggtgcaGCAgtagttcttGCAaatggtcaatataacatcaatagcaaaga" description="0"><BindingSite location="6172-6221" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6172-6177" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6180-6189" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6193-6221" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite><BindingSite simplified="1" location="6172-6221" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6172-6177" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6180-6189" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6193-6221" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite></Primer><Primer recentID="36" name="P692 PriM_mpk_R1" sequence="TGCaagaactacTGCtgcaccTGCgaacatatcaatgttgcttctgtagtc" description="0"><BindingSite location="6142-6189" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6180-6189" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6172-6177" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6142-6168" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite><BindingSite simplified="1" location="6142-6189" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6180-6189" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6172-6177" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6142-6168" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite></Primer><Primer recentID="35" name="P365 FTL0702cmp_R1" sequence="atatGCGGCCGCttcttctttaggcaaaacattacttaag" description="Contains NotI site"><BindingSite location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="34" name="P694 PriM_mpk_R2" sequence="GGTACCaagctatgaacatgattatagctttagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7203-7236" boundStrand="1" annealedBases="GGTACCaagctatgaacatgattatagctttagc" meltingTemperature="60"><Component hybridizedRange="7203-7236" bases="GGTACCaagctatgaacatgattatagctttagc"/></BindingSite><BindingSite simplified="1" location="7203-7236" boundStrand="1" annealedBases="GGTACCaagctatgaacatgattatagctttagc" meltingTemperature="60"><Component hybridizedRange="7203-7236" bases="GGTACCaagctatgaacatgattatagctttagc"/></BindingSite></Primer><Primer recentID="33" name="P433 FTL0702cmp_R2" sequence="atatGGATCCtcattcttctttaggcaaaacattactt" description="Contains BamHI site"><BindingSite location="6945-6972" boundStrand="1" annealedBases="tcattcttctttaggcaaaacattactt" meltingTemperature="55"><Component bases="atatGGATCC"/><Component hybridizedRange="6945-6972" bases="tcattcttctttaggcaaaacattactt"/></BindingSite><BindingSite simplified="1" location="6945-6972" boundStrand="1" annealedBases="tcattcttctttaggcaaaacattactt" meltingTemperature="55"><Component bases="atatGGATCC"/><Component hybridizedRange="6945-6972" bases="tcattcttctttaggcaaaacattactt"/></BindingSite></Primer><Primer recentID="32" name="P366 FTL0702cmp_R2" sequence="atatGGATCCttcttctttaggcaaaacattacttaag" description="&quot;Contains BamHI site, could use to make vector lacking VSVG tag&quot;"><BindingSite location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGGATCC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGGATCC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="2" name="P444 pKL75_F" sequence="atatGGTACCagcttaatcagtatcagaactaatacc" description="Contains KpnI site"><BindingSite location="6430-6456" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6430-6456" bases="agcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6430-6456" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6430-6456" bases="agcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="8" name="P695 PriM_mpk_R3" sequence="catcagcTGCtagtgaaactgctttagttgtatcact" description="0"><BindingSite location="6295-6331" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6325-6331" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6295-6321" bases="tagtgaaactgctttagttgtatcact"/></BindingSite><BindingSite simplified="1" location="6295-6331" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6325-6331" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6295-6321" bases="tagtgaaactgctttagttgtatcact"/></BindingSite></Primer><Primer recentID="31" name="P696 PriM_mpk_F4" sequence="ttcactaGCAgctgatgctaacacacttacatatac" description="0"><BindingSite location="6325-6350" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6325-6350" bases="gctgatgctaacacacttacatatac"/></BindingSite><BindingSite simplified="1" location="6325-6350" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6325-6350" bases="gctgatgctaacacacttacatatac"/></BindingSite></Primer><Primer recentID="22" name="P702 PriM_mtip1_R2" sequence="atatGGTACCtactttctcttcgaaaacggtgtcg" description="Contains KpnI site"><BindingSite location="6885-6909" boundStrand="1" annealedBases="tactttctcttcgaaaacggtgtcg" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6885-6909" bases="tactttctcttcgaaaacggtgtcg"/></BindingSite><BindingSite simplified="1" location="6885-6909" boundStrand="1" annealedBases="tactttctcttcgaaaacggtgtcg" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6885-6909" bases="tactttctcttcgaaaacggtgtcg"/></BindingSite></Primer><Primer recentID="0" name="P411 FTL0702sp_2R" sequence="atatGGTACCgcatttgtagctgctttaagtgccc" description="Contains KpnI site"><BindingSite location="6593-6617" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="6593-6617" bases="gcatttgtagctgctttaagtgccc"/></BindingSite><BindingSite simplified="1" location="6593-6617" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="6593-6617" bases="gcatttgtagctgctttaagtgccc"/></BindingSite></Primer><Primer recentID="3" name="P368 FTL0702seqR1" sequence="acttgtagatattaacattaaagtatgac" description="0"><BindingSite location="6662-6690" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="acttgtagatattaacattaaagtatgac"/></BindingSite><BindingSite simplified="1" location="6662-6690" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="acttgtagatattaacattaaagtatgac"/></BindingSite></Primer><Primer recentID="4" name="P430 CkFTL0702V_R" sequence="gtcatactttaatgttaatatctacaagt" description="0"><BindingSite location="6662-6690" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite><BindingSite simplified="1" location="6662-6690" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite></Primer><Primer recentID="5" name="P329 SP_D0702-R" sequence="aatgatgacaataaaatagaccatttgg" description="0"><BindingSite location="7063-7090" boundStrand="0" annealedBases="aatgatgacaataaaatagaccatttgg" meltingTemperature="55"><Component hybridizedRange="7063-7090" bases="aatgatgacaataaaatagaccatttgg"/></BindingSite><BindingSite simplified="1" location="7063-7090" boundStrand="0" annealedBases="aatgatgacaataaaatagaccatttgg" meltingTemperature="55"><Component hybridizedRange="7063-7090" bases="aatgatgacaataaaatagaccatttgg"/></BindingSite></Primer><Primer recentID="6" name="P697 Ck_PriM_mpk1F" sequence="cagcatgggattctgctactagtc" description="0"><BindingSite location="6107-6130" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="58"><Component hybridizedRange="6107-6130" bases="cagcatgggattctgctactagtc"/></BindingSite><BindingSite simplified="1" location="6107-6130" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="58"><Component hybridizedRange="6107-6130" bases="cagcatgggattctgctactagtc"/></BindingSite></Primer><Primer recentID="7" name="P437 RT_FTL0702-2F" sequence="agcaagtaagtgatgctgataga" description="0"><BindingSite location="6740-6762" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="6740-6762" bases="agcaagtaagtgatgctgataga"/></BindingSite><BindingSite simplified="1" location="6740-6762" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="6740-6762" bases="agcaagtaagtgatgctgataga"/></BindingSite></Primer><Primer recentID="9" name="P698 Ck_PriM_mpk1R" sequence="gctttaagtgcccagttgtcgcc" description="0"><BindingSite location="6583-6605" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="6583-6605" bases="gctttaagtgcccagttgtcgcc"/></BindingSite><BindingSite simplified="1" location="6583-6605" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="6583-6605" bases="gctttaagtgcccagttgtcgcc"/></BindingSite></Primer><Primer recentID="10" name="P438 RT_FTL0702-2R" sequence="agttgtccggctatctgtttag" description="0"><BindingSite location="6845-6866" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="6845-6866" bases="agttgtccggctatctgtttag"/></BindingSite><BindingSite simplified="1" location="6845-6866" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="6845-6866" bases="agttgtccggctatctgtttag"/></BindingSite></Primer><Primer recentID="11" name="P439 RT_FTL0702-3F" sequence="tgctgatgctaacacacttaca" description="0"><BindingSite location="6324-6345" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6324-6345" bases="tgctgatgctaacacacttaca"/></BindingSite><BindingSite simplified="1" location="6324-6345" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6324-6345" bases="tgctgatgctaacacacttaca"/></BindingSite></Primer><Primer recentID="13" name="P436 RT_FTL0702-1R" sequence="cggcatctttgctattgatgtt" description="0"><BindingSite location="6205-6226" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6205-6226" bases="cggcatctttgctattgatgtt"/></BindingSite><BindingSite simplified="1" location="6205-6226" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6205-6226" bases="cggcatctttgctattgatgtt"/></BindingSite></Primer><Primer recentID="14" name="P440 RT_FTL0702-3R" sequence="ccgttcttggcgaagctatt" description="0"><BindingSite location="6390-6409" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6390-6409" bases="ccgttcttggcgaagctatt"/></BindingSite><BindingSite simplified="1" location="6390-6409" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6390-6409" bases="ccgttcttggcgaagctatt"/></BindingSite></Primer><Primer recentID="15" name="P435 RT_FTL0702-1F" sequence="cagcatgggattctgctact" description="0"><BindingSite location="6107-6126" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6107-6126" bases="cagcatgggattctgctact"/></BindingSite><BindingSite simplified="1" location="6107-6126" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6107-6126" bases="cagcatgggattctgctact"/></BindingSite></Primer><Primer recentID="16" name="KROL20 PriMnoC1FR1R" sequence="aattgatagagccgttcttggcgaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6396-6420" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6396-6420" bases="aattgatagagccgttcttggcgaa"/></BindingSite><BindingSite simplified="1" location="6396-6420" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6396-6420" bases="aattgatagagccgttcttggcgaa"/></BindingSite></Primer><Primer recentID="17" name="KROL21 PriMnoC1FR2F" sequence="atatGCtgaagctagcttaatcagtatcagaactaatacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6423-6456" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="58"><Component bases="atatGC"/><Component hybridizedRange="6423-6456" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6423-6456" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="58"><Component bases="atatGC"/><Component hybridizedRange="6423-6456" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="18" name="KROL23 PriMnoC2FR2F" sequence="aaaaatgcaGCAaattttacagcagctaaaaatagtaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6775-6814" boundStrand="0" annealedBases="aaaaatgcaaattttacagcagctaaaaatagtaaca" meltingTemperature="53"><Component hybridizedRange="6775-6783" bases="aaaaatgca"/><Component bases="GCA"/><Component hybridizedRange="6787-6814" bases="aattttacagcagctaaaaatagtaaca"/></BindingSite><BindingSite simplified="1" location="6775-6814" boundStrand="0" annealedBases="aaaaatgcaaattttacagcagctaaaaatagtaaca" meltingTemperature="53"><Component hybridizedRange="6775-6783" bases="aaaaatgca"/><Component bases="GCA"/><Component hybridizedRange="6787-6814" bases="aattttacagcagctaaaaatagtaaca"/></BindingSite></Primer><Primer recentID="19" name="KROL22 PriMnoC2FR1R" sequence="tgtaaaattaGCAgcatttttaccaaatagaactctatca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6756-6795" boundStrand="1" annealedBases="tgtaaaattagcatttttaccaaatagaactctatca" meltingTemperature="50"><Component hybridizedRange="6786-6795" bases="tgtaaaatta"/><Component bases="GCA"/><Component hybridizedRange="6756-6782" bases="gcatttttaccaaatagaactctatca"/></BindingSite><BindingSite simplified="1" location="6756-6795" boundStrand="1" annealedBases="tgtaaaattagcatttttaccaaatagaactctatca" meltingTemperature="50"><Component hybridizedRange="6786-6795" bases="tgtaaaatta"/><Component bases="GCA"/><Component hybridizedRange="6756-6782" bases="gcatttttaccaaatagaactctatca"/></BindingSite></Primer><Primer recentID="20" name="KROL24 PriMnoC2FR2R" sequence="atatGGTACCtgtcgaaagtgattcttagattcagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7002-7027" boundStrand="1" annealedBases="tgtcgaaagtgattcttagattcagc" meltingTemperature="56"><Component bases="atatGGTACC"/><Component hybridizedRange="7002-7027" bases="tgtcgaaagtgattcttagattcagc"/></BindingSite><BindingSite simplified="1" location="7002-7027" boundStrand="1" annealedBases="tgtcgaaagtgattcttagattcagc" meltingTemperature="56"><Component bases="atatGGTACC"/><Component hybridizedRange="7002-7027" bases="tgtcgaaagtgattcttagattcagc"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="21:3:2">2018.12.17</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NA'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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  <?xml version="1.0"?><Primers recycledIDs="20,19,18,17,16,15,14,13,11,10,9,7,6,5,4,3,0,22,31,8,2,32,33,34,35,36,38,39,27,28,21,1,26,24,23,30,29,25,37,12" nextValidID="40"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="21:2:19">2018.12.17</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
          M        M.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAMky.E5I+Ymd=j\f^zkGR	!ySJٝ%zRHU֢m&yg)NEf]mYUN%_m^Ii1׀ޕ4V5mu&yXI&
PaZMIHcDj	U5j4VIudNaN]<zP)*DT:my^*X^XyZVE	;[b8!Z)ֽvq<R%O#億Z2Wial=s0C
;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%        s  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7055-7071" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  P<?xml version="1.0"?><Primers recycledIDs="1,26,24,23,30,29,25,37,12" nextValidID="40"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="21" name="P821 PriM_mtip1_R3" sequence="atatGGTACCgtaactttggtattagttctgatactg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6438-6464" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6438-6464" bases="gtaactttggtattagttctgatactg"/></BindingSite><BindingSite simplified="1" location="6438-6464" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6438-6464" bases="gtaactttggtattagttctgatactg"/></BindingSite></Primer><Primer recentID="28" name="P819 PriM_mpk_R2" sequence="atatGGTACCtgtaaagccaaatatactttctcttcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6897-6923" boundStrand="1" annealedBases="tgtaaagccaaatatactttctcttcg" meltingTemperature="54"><Component bases="atatGGTACC"/><Component hybridizedRange="6897-6923" bases="tgtaaagccaaatatactttctcttcg"/></BindingSite><BindingSite simplified="1" location="6897-6923" boundStrand="1" annealedBases="tgtaaagccaaatatactttctcttcg" meltingTemperature="54"><Component bases="atatGGTACC"/><Component hybridizedRange="6897-6923" bases="tgtaaagccaaatatactttctcttcg"/></BindingSite></Primer><Primer recentID="27" name="P707 PriM_unst1_R1" sequence="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6135-6181" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6172-6181" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6163-6168" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6135-6161" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite><BindingSite simplified="1" location="6135-6181" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6172-6181" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6163-6168" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6135-6161" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite></Primer><Primer recentID="39" name="P708 PriM_unst1_F1" sequence="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6152-6196" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6152-6161" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6163-6168" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6172-6196" bases="ggtgcaagagtagttctttggaatg"/></BindingSite><BindingSite simplified="1" location="6152-6196" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6152-6161" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6163-6168" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6172-6196" bases="ggtgcaagagtagttctttggaatg"/></BindingSite></Primer><Primer recentID="38" name="P428 FTL0702_VSVG_2F" sequence="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGCttcttctttaggcaaaacattacttaag" description="0"><BindingSite location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="36" name="P427 FTL0702_VSVG_1R" sequence="GATATTGAAATGAATAGATTAGGTAAATAAactaatgactaaatttctaaaattaattag" description="0"><BindingSite location="6972-7002" boundStrand="0" annealedBases="Aactaatgactaaatttctaaaattaattag" meltingTemperature="51"><Component bases="GATATTGAAATGAATAGATTAGGTAAATA"/><Component hybridizedRange="6972-7002" bases="Aactaatgactaaatttctaaaattaattag"/></BindingSite><BindingSite simplified="1" location="6972-7002" boundStrand="0" annealedBases="Aactaatgactaaatttctaaaattaattag" meltingTemperature="51"><Component bases="GATATTGAAATGAATAGATTAGGTAAATA"/><Component hybridizedRange="6972-7002" bases="Aactaatgactaaatttctaaaattaattag"/></BindingSite></Primer><Primer recentID="35" name="P693 PriM_mpk_F2" sequence="GCAggtgcaGCAgtagttcttGCAaatggtcaatataacatcaatagcaaaga" description="0"><BindingSite location="6172-6221" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6172-6177" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6180-6189" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6193-6221" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite><BindingSite simplified="1" location="6172-6221" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6172-6177" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6180-6189" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6193-6221" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite></Primer><Primer recentID="34" name="P692 PriM_mpk_R1" sequence="TGCaagaactacTGCtgcaccTGCgaacatatcaatgttgcttctgtagtc" description="0"><BindingSite location="6142-6189" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6180-6189" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6172-6177" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6142-6168" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite><BindingSite simplified="1" location="6142-6189" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6180-6189" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6172-6177" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6142-6168" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite></Primer><Primer recentID="33" name="P365 FTL0702cmp_R1" sequence="atatGCGGCCGCttcttctttaggcaaaacattacttaag" description="Contains NotI site"><BindingSite location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="32" name="P433 FTL0702cmp_R2" sequence="atatGGATCCtcattcttctttaggcaaaacattactt" description="Contains BamHI site"><BindingSite location="6945-6972" boundStrand="1" annealedBases="tcattcttctttaggcaaaacattactt" meltingTemperature="55"><Component bases="atatGGATCC"/><Component hybridizedRange="6945-6972" bases="tcattcttctttaggcaaaacattactt"/></BindingSite><BindingSite simplified="1" location="6945-6972" boundStrand="1" annealedBases="tcattcttctttaggcaaaacattactt" meltingTemperature="55"><Component bases="atatGGATCC"/><Component hybridizedRange="6945-6972" bases="tcattcttctttaggcaaaacattactt"/></BindingSite></Primer><Primer recentID="2" name="P366 FTL0702cmp_R2" sequence="atatGGATCCttcttctttaggcaaaacattacttaag" description="&quot;Contains BamHI site, could use to make vector lacking VSVG tag&quot;"><BindingSite location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGGATCC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGGATCC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="8" name="P444 pKL75_F" sequence="atatGGTACCagcttaatcagtatcagaactaatacc" description="Contains KpnI site"><BindingSite location="6430-6456" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6430-6456" bases="agcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6430-6456" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6430-6456" bases="agcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="31" name="P695 PriM_mpk_R3" sequence="catcagcTGCtagtgaaactgctttagttgtatcact" description="0"><BindingSite location="6295-6331" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6325-6331" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6295-6321" bases="tagtgaaactgctttagttgtatcact"/></BindingSite><BindingSite simplified="1" location="6295-6331" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6325-6331" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6295-6321" bases="tagtgaaactgctttagttgtatcact"/></BindingSite></Primer><Primer recentID="22" name="P696 PriM_mpk_F4" sequence="ttcactaGCAgctgatgctaacacacttacatatac" description="0"><BindingSite location="6325-6350" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6325-6350" bases="gctgatgctaacacacttacatatac"/></BindingSite><BindingSite simplified="1" location="6325-6350" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6325-6350" bases="gctgatgctaacacacttacatatac"/></BindingSite></Primer><Primer recentID="0" name="P702 PriM_mtip1_R2" sequence="atatGGTACCtactttctcttcgaaaacggtgtcg" description="Contains KpnI site"><BindingSite location="6885-6909" boundStrand="1" annealedBases="tactttctcttcgaaaacggtgtcg" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6885-6909" bases="tactttctcttcgaaaacggtgtcg"/></BindingSite><BindingSite simplified="1" location="6885-6909" boundStrand="1" annealedBases="tactttctcttcgaaaacggtgtcg" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6885-6909" bases="tactttctcttcgaaaacggtgtcg"/></BindingSite></Primer><Primer recentID="3" name="P411 FTL0702sp_2R" sequence="atatGGTACCgcatttgtagctgctttaagtgccc" description="Contains KpnI site"><BindingSite location="6593-6617" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="6593-6617" bases="gcatttgtagctgctttaagtgccc"/></BindingSite><BindingSite simplified="1" location="6593-6617" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="6593-6617" bases="gcatttgtagctgctttaagtgccc"/></BindingSite></Primer><Primer recentID="4" name="P368 FTL0702seqR1" sequence="acttgtagatattaacattaaagtatgac" description="0"><BindingSite location="6662-6690" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="acttgtagatattaacattaaagtatgac"/></BindingSite><BindingSite simplified="1" location="6662-6690" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="acttgtagatattaacattaaagtatgac"/></BindingSite></Primer><Primer recentID="5" name="P430 CkFTL0702V_R" sequence="gtcatactttaatgttaatatctacaagt" description="0"><BindingSite location="6662-6690" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite><BindingSite simplified="1" location="6662-6690" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite></Primer><Primer recentID="6" name="P697 Ck_PriM_mpk1F" sequence="cagcatgggattctgctactagtc" description="0"><BindingSite location="6107-6130" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="58"><Component hybridizedRange="6107-6130" bases="cagcatgggattctgctactagtc"/></BindingSite><BindingSite simplified="1" location="6107-6130" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="58"><Component hybridizedRange="6107-6130" bases="cagcatgggattctgctactagtc"/></BindingSite></Primer><Primer recentID="7" name="P437 RT_FTL0702-2F" sequence="agcaagtaagtgatgctgataga" description="0"><BindingSite location="6740-6762" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="6740-6762" bases="agcaagtaagtgatgctgataga"/></BindingSite><BindingSite simplified="1" location="6740-6762" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="6740-6762" bases="agcaagtaagtgatgctgataga"/></BindingSite></Primer><Primer recentID="9" name="P698 Ck_PriM_mpk1R" sequence="gctttaagtgcccagttgtcgcc" description="0"><BindingSite location="6583-6605" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="6583-6605" bases="gctttaagtgcccagttgtcgcc"/></BindingSite><BindingSite simplified="1" location="6583-6605" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="6583-6605" bases="gctttaagtgcccagttgtcgcc"/></BindingSite></Primer><Primer recentID="10" name="P438 RT_FTL0702-2R" sequence="agttgtccggctatctgtttag" description="0"><BindingSite location="6845-6866" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="6845-6866" bases="agttgtccggctatctgtttag"/></BindingSite><BindingSite simplified="1" location="6845-6866" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="6845-6866" bases="agttgtccggctatctgtttag"/></BindingSite></Primer><Primer recentID="11" name="P439 RT_FTL0702-3F" sequence="tgctgatgctaacacacttaca" description="0"><BindingSite location="6324-6345" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6324-6345" bases="tgctgatgctaacacacttaca"/></BindingSite><BindingSite simplified="1" location="6324-6345" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6324-6345" bases="tgctgatgctaacacacttaca"/></BindingSite></Primer><Primer recentID="13" name="P436 RT_FTL0702-1R" sequence="cggcatctttgctattgatgtt" description="0"><BindingSite location="6205-6226" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6205-6226" bases="cggcatctttgctattgatgtt"/></BindingSite><BindingSite simplified="1" location="6205-6226" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6205-6226" bases="cggcatctttgctattgatgtt"/></BindingSite></Primer><Primer recentID="14" name="P440 RT_FTL0702-3R" sequence="ccgttcttggcgaagctatt" description="0"><BindingSite location="6390-6409" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6390-6409" bases="ccgttcttggcgaagctatt"/></BindingSite><BindingSite simplified="1" location="6390-6409" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6390-6409" bases="ccgttcttggcgaagctatt"/></BindingSite></Primer><Primer recentID="15" name="P435 RT_FTL0702-1F" sequence="cagcatgggattctgctact" description="0"><BindingSite location="6107-6126" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6107-6126" bases="cagcatgggattctgctact"/></BindingSite><BindingSite simplified="1" location="6107-6126" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6107-6126" bases="cagcatgggattctgctact"/></BindingSite></Primer><Primer recentID="16" name="KROL20 PriMnoC1FR1R" sequence="aattgatagagccgttcttggcgaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6396-6420" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6396-6420" bases="aattgatagagccgttcttggcgaa"/></BindingSite><BindingSite simplified="1" location="6396-6420" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6396-6420" bases="aattgatagagccgttcttggcgaa"/></BindingSite></Primer><Primer recentID="17" name="KROL21 PriMnoC1FR2F" sequence="atatGCtgaagctagcttaatcagtatcagaactaatacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6423-6456" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="58"><Component bases="atatGC"/><Component hybridizedRange="6423-6456" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6423-6456" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="58"><Component bases="atatGC"/><Component hybridizedRange="6423-6456" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="18" name="KROL23 PriMnoC2FR2F" sequence="aaaaatgcaGCAaattttacagcagctaaaaatagtaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6775-6814" boundStrand="0" annealedBases="aaaaatgcaGCAaattttacagcagctaaaaatagtaaca" meltingTemperature="62"><Component hybridizedRange="6775-6814" bases="aaaaatgcaGCAaattttacagcagctaaaaatagtaaca"/></BindingSite><BindingSite simplified="1" location="6775-6814" boundStrand="0" annealedBases="aaaaatgcaGCAaattttacagcagctaaaaatagtaaca" meltingTemperature="62"><Component hybridizedRange="6775-6814" bases="aaaaatgcaGCAaattttacagcagctaaaaatagtaaca"/></BindingSite></Primer><Primer recentID="19" name="KROL22 PriMnoC2FR1R" sequence="tgtaaaattaGCAgcatttttaccaaatagaactctatca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6756-6795" boundStrand="1" annealedBases="tgtaaaattGCgcatttttaccaaatagaactctatca" meltingTemperature="53"><Component hybridizedRange="6787-6795" bases="tgtaaaatt"/><Component bases="a"/><Component hybridizedRange="6784-6785" bases="GC"/><Component bases="A"/><Component hybridizedRange="6756-6782" bases="gcatttttaccaaatagaactctatca"/></BindingSite><BindingSite simplified="1" location="6756-6795" boundStrand="1" annealedBases="tgtaaaattGCgcatttttaccaaatagaactctatca" meltingTemperature="53"><Component hybridizedRange="6787-6795" bases="tgtaaaatt"/><Component bases="a"/><Component hybridizedRange="6784-6785" bases="GC"/><Component bases="A"/><Component hybridizedRange="6756-6782" bases="gcatttttaccaaatagaactctatca"/></BindingSite></Primer><Primer recentID="20" name="KROL24 PriMnoC2FR2R" sequence="atatGGTACCtgtcgaaagtgattcttagattcagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7002-7033" boundStrand="1" annealedBases="GGTACCtgtcgaaagtgattcttagattcagc" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="7002-7033" bases="GGTACCtgtcgaaagtgattcttagattcagc"/></BindingSite><BindingSite simplified="1" location="7002-7033" boundStrand="1" annealedBases="GGTACCtgtcgaaagtgattcttagattcagc" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="7002-7033" bases="GGTACCtgtcgaaagtgattcttagattcagc"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="21:0:1">2018.12.17</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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PaZMIHcDj	Uj4VIudNaN]<zP)*DT:my^*X^XyZVE	;[b8!Z)ֽvq<R%O#億Z2Wial=s0C
;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%    s  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  0<?xml version="1.0"?><Features recycledIDs="0,3,7,8,9,45,44,46,43,42" nextValidID="47"><Feature recentID="41" name="P708 PriM_unst1_F1" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6173-6197" color="#50ff50" type="standard"/><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature><Feature recentID="40" name="P707 PriM_unst1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6136-6162" color="#50ff50" type="standard"/><Q name="note"><V text="P707 PriM_unst1_R1"/></Q></Feature><Feature recentID="39" name="P702 PriM_mtip1_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6886-6910" color="#50ff50" type="standard"/><Q name="note"><V text="P702 PriM_mtip1_R2"/></Q></Feature><Feature recentID="25" name="P697 Ck_PriM_mpk1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6131" color="#50ff50" type="standard"/><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature><Feature recentID="24" name="P698 Ck_PriM_mpk1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6584-6606" color="#50ff50" type="standard"/><Q name="note"><V text="P698 Ck_PriM_mpk1R"/></Q></Feature><Feature recentID="23" name="P693 PriM_mpk_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6194-6222" color="#50ff50" type="standard"/><Q name="note"><V text="P693 PriM_mpk_F2"/></Q></Feature><Feature recentID="22" name="P692 PriM_mpk_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6143-6169" color="#50ff50" type="standard"/><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="21" name="P444 pKL75_F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6431-6457" color="#50ff50" type="standard"/><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="20" name="P440 RT_FTL0702-3R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6391-6410" color="#50ff50" type="standard"/><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="19" name="P439 RT_FTL0702-3F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6325-6346" color="#50ff50" type="standard"/><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="18" name="P438 RT_FTL0702-2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6846-6867" color="#50ff50" type="standard"/><Q name="note"><V text="P438 RT_FTL0702-2R"/></Q></Feature><Feature recentID="17" name="P437 RT_FTL0702-2F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6741-6763" color="#50ff50" type="standard"/><Q name="note"><V text="P437 RT_FTL0702-2F"/></Q></Feature><Feature recentID="16" name="P436 RT_FTL0702-1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6206-6227" color="#50ff50" type="standard"/><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="15" name="P435 RT_FTL0702-1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6127" color="#50ff50" type="standard"/><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="14" name="P433 FTL0702cmp_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6946-6973" color="#50ff50" type="standard"/><Q name="note"><V text="P433 FTL0702cmp_R2"/></Q></Feature><Feature recentID="13" name="P428 FTL0702_VSVG_2F" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6943-6970" color="#50ff50" type="standard"/><Q name="note"><V text="P428 FTL0702_VSVG_2F"/></Q></Feature><Feature recentID="12" name="P411 FTL0702sp_2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6594-6618" color="#50ff50" type="standard"/><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="11" name="P368 FTL0702seqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6663-6691" color="#8080ff" type="standard"/><Q name="note"><V text="P368 FTL0702seqR1"/></Q></Feature><Feature recentID="10" name="P365 FTL0702cmp_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6943-6970" color="#8080ff" type="standard"/><Q name="note"><V text="P365 FTL0702cmp_R"/></Q></Feature><Feature recentID="6" name="hypothetical protein" directionality="1" translationMW="31575.01" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="199" hitsStopCodon="1"><Segment range="6108-6973" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6973" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="P427 FTL0702_VSVG_1R" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6974-7003" color="#50ff50" type="standard"/><Q name="note"><V text="P427 FTL0702_VSVG_1R"/></Q></Feature><Feature recentID="2" name="RPp7" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6964-6992" color="#0080ff" type="standard"/><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-7028" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="38" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7757" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="mol_type"><V predef="genomic DNA"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="37" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7029-7033" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="36" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7463-288" color="#ffff00" type="standard"/><Q name="note"><V text="ColE origin"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="35" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="34" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="32" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="30" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7055-7071" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  P<?xml version="1.0"?><Primers recycledIDs="1,26,24,23,30,29,25,37,12" nextValidID="40"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="21" name="P821 PriM_mtip1_R3" sequence="atatGGTACCgtaactttggtattagttctgatactg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6438-6464" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6438-6464" bases="gtaactttggtattagttctgatactg"/></BindingSite><BindingSite simplified="1" location="6438-6464" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6438-6464" bases="gtaactttggtattagttctgatactg"/></BindingSite></Primer><Primer recentID="28" name="P819 PriM_mpk_R2" sequence="atatGGTACCtgtaaagccaaatatactttctcttcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6897-6923" boundStrand="1" annealedBases="tgtaaagccaaatatactttctcttcg" meltingTemperature="54"><Component bases="atatGGTACC"/><Component hybridizedRange="6897-6923" bases="tgtaaagccaaatatactttctcttcg"/></BindingSite><BindingSite simplified="1" location="6897-6923" boundStrand="1" annealedBases="tgtaaagccaaatatactttctcttcg" meltingTemperature="54"><Component bases="atatGGTACC"/><Component hybridizedRange="6897-6923" bases="tgtaaagccaaatatactttctcttcg"/></BindingSite></Primer><Primer recentID="27" name="P707 PriM_unst1_R1" sequence="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6135-6181" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6172-6181" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6163-6168" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6135-6161" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite><BindingSite simplified="1" location="6135-6181" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6172-6181" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6163-6168" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6135-6161" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite></Primer><Primer recentID="39" name="P708 PriM_unst1_F1" sequence="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6152-6196" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6152-6161" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6163-6168" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6172-6196" bases="ggtgcaagagtagttctttggaatg"/></BindingSite><BindingSite simplified="1" location="6152-6196" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6152-6161" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6163-6168" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6172-6196" bases="ggtgcaagagtagttctttggaatg"/></BindingSite></Primer><Primer recentID="38" name="P428 FTL0702_VSVG_2F" sequence="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGCttcttctttaggcaaaacattacttaag" description="0"><BindingSite location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="36" name="P427 FTL0702_VSVG_1R" sequence="GATATTGAAATGAATAGATTAGGTAAATAAactaatgactaaatttctaaaattaattag" description="0"><BindingSite location="6972-7002" boundStrand="0" annealedBases="Aactaatgactaaatttctaaaattaattag" meltingTemperature="51"><Component bases="GATATTGAAATGAATAGATTAGGTAAATA"/><Component hybridizedRange="6972-7002" bases="Aactaatgactaaatttctaaaattaattag"/></BindingSite><BindingSite simplified="1" location="6972-7002" boundStrand="0" annealedBases="Aactaatgactaaatttctaaaattaattag" meltingTemperature="51"><Component bases="GATATTGAAATGAATAGATTAGGTAAATA"/><Component hybridizedRange="6972-7002" bases="Aactaatgactaaatttctaaaattaattag"/></BindingSite></Primer><Primer recentID="35" name="P693 PriM_mpk_F2" sequence="GCAggtgcaGCAgtagttcttGCAaatggtcaatataacatcaatagcaaaga" description="0"><BindingSite location="6172-6221" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6172-6177" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6180-6189" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6193-6221" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite><BindingSite simplified="1" location="6172-6221" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6172-6177" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6180-6189" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6193-6221" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite></Primer><Primer recentID="34" name="P692 PriM_mpk_R1" sequence="TGCaagaactacTGCtgcaccTGCgaacatatcaatgttgcttctgtagtc" description="0"><BindingSite location="6142-6189" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6180-6189" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6172-6177" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6142-6168" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite><BindingSite simplified="1" location="6142-6189" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6180-6189" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6172-6177" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6142-6168" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite></Primer><Primer recentID="33" name="P365 FTL0702cmp_R1" sequence="atatGCGGCCGCttcttctttaggcaaaacattacttaag" description="Contains NotI site"><BindingSite location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="32" name="P433 FTL0702cmp_R2" sequence="atatGGATCCtcattcttctttaggcaaaacattactt" description="Contains BamHI site"><BindingSite location="6945-6972" boundStrand="1" annealedBases="tcattcttctttaggcaaaacattactt" meltingTemperature="55"><Component bases="atatGGATCC"/><Component hybridizedRange="6945-6972" bases="tcattcttctttaggcaaaacattactt"/></BindingSite><BindingSite simplified="1" location="6945-6972" boundStrand="1" annealedBases="tcattcttctttaggcaaaacattactt" meltingTemperature="55"><Component bases="atatGGATCC"/><Component hybridizedRange="6945-6972" bases="tcattcttctttaggcaaaacattactt"/></BindingSite></Primer><Primer recentID="2" name="P366 FTL0702cmp_R2" sequence="atatGGATCCttcttctttaggcaaaacattacttaag" description="&quot;Contains BamHI site, could use to make vector lacking VSVG tag&quot;"><BindingSite location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGGATCC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="6942-6969" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGGATCC"/><Component hybridizedRange="6942-6969" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="8" name="P444 pKL75_F" sequence="atatGGTACCagcttaatcagtatcagaactaatacc" description="Contains KpnI site"><BindingSite location="6430-6456" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6430-6456" bases="agcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6430-6456" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6430-6456" bases="agcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="31" name="P695 PriM_mpk_R3" sequence="catcagcTGCtagtgaaactgctttagttgtatcact" description="0"><BindingSite location="6295-6331" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6325-6331" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6295-6321" bases="tagtgaaactgctttagttgtatcact"/></BindingSite><BindingSite simplified="1" location="6295-6331" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6325-6331" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6295-6321" bases="tagtgaaactgctttagttgtatcact"/></BindingSite></Primer><Primer recentID="22" name="P696 PriM_mpk_F4" sequence="ttcactaGCAgctgatgctaacacacttacatatac" description="0"><BindingSite location="6325-6350" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6325-6350" bases="gctgatgctaacacacttacatatac"/></BindingSite><BindingSite simplified="1" location="6325-6350" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6325-6350" bases="gctgatgctaacacacttacatatac"/></BindingSite></Primer><Primer recentID="0" name="P702 PriM_mtip1_R2" sequence="atatGGTACCtactttctcttcgaaaacggtgtcg" description="Contains KpnI site"><BindingSite location="6885-6909" boundStrand="1" annealedBases="tactttctcttcgaaaacggtgtcg" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6885-6909" bases="tactttctcttcgaaaacggtgtcg"/></BindingSite><BindingSite simplified="1" location="6885-6909" boundStrand="1" annealedBases="tactttctcttcgaaaacggtgtcg" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6885-6909" bases="tactttctcttcgaaaacggtgtcg"/></BindingSite></Primer><Primer recentID="3" name="P411 FTL0702sp_2R" sequence="atatGGTACCgcatttgtagctgctttaagtgccc" description="Contains KpnI site"><BindingSite location="6593-6617" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="6593-6617" bases="gcatttgtagctgctttaagtgccc"/></BindingSite><BindingSite simplified="1" location="6593-6617" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="6593-6617" bases="gcatttgtagctgctttaagtgccc"/></BindingSite></Primer><Primer recentID="4" name="P368 FTL0702seqR1" sequence="acttgtagatattaacattaaagtatgac" description="0"><BindingSite location="6662-6690" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="acttgtagatattaacattaaagtatgac"/></BindingSite><BindingSite simplified="1" location="6662-6690" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="acttgtagatattaacattaaagtatgac"/></BindingSite></Primer><Primer recentID="5" name="P430 CkFTL0702V_R" sequence="gtcatactttaatgttaatatctacaagt" description="0"><BindingSite location="6662-6690" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite><BindingSite simplified="1" location="6662-6690" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="6662-6690" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite></Primer><Primer recentID="6" name="P697 Ck_PriM_mpk1F" sequence="cagcatgggattctgctactagtc" description="0"><BindingSite location="6107-6130" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="58"><Component hybridizedRange="6107-6130" bases="cagcatgggattctgctactagtc"/></BindingSite><BindingSite simplified="1" location="6107-6130" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="58"><Component hybridizedRange="6107-6130" bases="cagcatgggattctgctactagtc"/></BindingSite></Primer><Primer recentID="7" name="P437 RT_FTL0702-2F" sequence="agcaagtaagtgatgctgataga" description="0"><BindingSite location="6740-6762" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="6740-6762" bases="agcaagtaagtgatgctgataga"/></BindingSite><BindingSite simplified="1" location="6740-6762" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="6740-6762" bases="agcaagtaagtgatgctgataga"/></BindingSite></Primer><Primer recentID="9" name="P698 Ck_PriM_mpk1R" sequence="gctttaagtgcccagttgtcgcc" description="0"><BindingSite location="6583-6605" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="6583-6605" bases="gctttaagtgcccagttgtcgcc"/></BindingSite><BindingSite simplified="1" location="6583-6605" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="6583-6605" bases="gctttaagtgcccagttgtcgcc"/></BindingSite></Primer><Primer recentID="10" name="P438 RT_FTL0702-2R" sequence="agttgtccggctatctgtttag" description="0"><BindingSite location="6845-6866" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="6845-6866" bases="agttgtccggctatctgtttag"/></BindingSite><BindingSite simplified="1" location="6845-6866" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="6845-6866" bases="agttgtccggctatctgtttag"/></BindingSite></Primer><Primer recentID="11" name="P439 RT_FTL0702-3F" sequence="tgctgatgctaacacacttaca" description="0"><BindingSite location="6324-6345" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6324-6345" bases="tgctgatgctaacacacttaca"/></BindingSite><BindingSite simplified="1" location="6324-6345" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6324-6345" bases="tgctgatgctaacacacttaca"/></BindingSite></Primer><Primer recentID="13" name="P436 RT_FTL0702-1R" sequence="cggcatctttgctattgatgtt" description="0"><BindingSite location="6205-6226" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6205-6226" bases="cggcatctttgctattgatgtt"/></BindingSite><BindingSite simplified="1" location="6205-6226" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6205-6226" bases="cggcatctttgctattgatgtt"/></BindingSite></Primer><Primer recentID="14" name="P440 RT_FTL0702-3R" sequence="ccgttcttggcgaagctatt" description="0"><BindingSite location="6390-6409" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6390-6409" bases="ccgttcttggcgaagctatt"/></BindingSite><BindingSite simplified="1" location="6390-6409" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6390-6409" bases="ccgttcttggcgaagctatt"/></BindingSite></Primer><Primer recentID="15" name="P435 RT_FTL0702-1F" sequence="cagcatgggattctgctact" description="0"><BindingSite location="6107-6126" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6107-6126" bases="cagcatgggattctgctact"/></BindingSite><BindingSite simplified="1" location="6107-6126" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6107-6126" bases="cagcatgggattctgctact"/></BindingSite></Primer><Primer recentID="16" name="KROL20 PriMnoC1FR1R" sequence="aattgatagagccgttcttggcgaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6396-6420" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6396-6420" bases="aattgatagagccgttcttggcgaa"/></BindingSite><BindingSite simplified="1" location="6396-6420" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6396-6420" bases="aattgatagagccgttcttggcgaa"/></BindingSite></Primer><Primer recentID="17" name="KROL21 PriMnoC1FR2F" sequence="atatGCtgaagctagcttaatcagtatcagaactaatacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6423-6456" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="58"><Component bases="atatGC"/><Component hybridizedRange="6423-6456" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6423-6456" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="58"><Component bases="atatGC"/><Component hybridizedRange="6423-6456" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="18" name="KROL23 PriMnoC2FR2F" sequence="aaaaatgcaGCAaattttacagcagctaaaaatagtaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6775-6814" boundStrand="0" annealedBases="aaaaatgcaaattttacagcagctaaaaatagtaaca" meltingTemperature="53"><Component hybridizedRange="6775-6783" bases="aaaaatgca"/><Component bases="GCA"/><Component hybridizedRange="6787-6814" bases="aattttacagcagctaaaaatagtaaca"/></BindingSite><BindingSite simplified="1" location="6775-6814" boundStrand="0" annealedBases="aaaaatgcaaattttacagcagctaaaaatagtaaca" meltingTemperature="53"><Component hybridizedRange="6775-6783" bases="aaaaatgca"/><Component bases="GCA"/><Component hybridizedRange="6787-6814" bases="aattttacagcagctaaaaatagtaaca"/></BindingSite></Primer><Primer recentID="19" name="KROL22 PriMnoC2FR1R" sequence="tgtaaaattaGCAgcatttttaccaaatagaactctatca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6756-6795" boundStrand="1" annealedBases="tgtaaaattagcatttttaccaaatagaactctatca" meltingTemperature="50"><Component hybridizedRange="6786-6795" bases="tgtaaaatta"/><Component bases="GCA"/><Component hybridizedRange="6756-6782" bases="gcatttttaccaaatagaactctatca"/></BindingSite><BindingSite simplified="1" location="6756-6795" boundStrand="1" annealedBases="tgtaaaattagcatttttaccaaatagaactctatca" meltingTemperature="50"><Component hybridizedRange="6786-6795" bases="tgtaaaatta"/><Component bases="GCA"/><Component hybridizedRange="6756-6782" bases="gcatttttaccaaatagaactctatca"/></BindingSite></Primer><Primer recentID="20" name="KROL24 PriMnoC2FR2R" sequence="atatGGTACCtgtcgaaagtgattcttagattcagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7002-7033" boundStrand="1" annealedBases="GGTACCtgtcgaaagtgattcttagattcagc" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="7002-7033" bases="GGTACCtgtcgaaagtgattcttagattcagc"/></BindingSite><BindingSite simplified="1" location="7002-7033" boundStrand="1" annealedBases="GGTACCtgtcgaaagtgattcttagattcagc" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="7002-7033" bases="GGTACCtgtcgaaagtgattcttagattcagc"/></BindingSite></Primer></Primers>
  D<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="20:58:56">2018.12.17</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NA'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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  <?xml version="1.0"?><Primers recycledIDs="20,19,18,17,16,15,14,13,11,10,9,7,6,5,4,3,0,22,31,8,2,32,33,34,35,36,38,39,27,28,21,1,26,24,23,30,29,25,37,12" nextValidID="40"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  D<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="20:58:51">2018.12.17</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  P     $            .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAjckU6M8cRYMXUB^]`)F|^UF׎;PY8TF]UTQZC$yU&yVFoBlUS8Ql*YUYX/2jmڕ69^bGhf+#Q$X-f]TcxqyyyiK$uM*f8cwftd1I"UVN\;ZSET9ityeU8~gwy&z~FV^zTӝX֑Bdrgbnu$(cmC8Eju5%!%V4tӕY%9:tduBԦ|Rfu*zRX>TQayajZZNެt'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ    :  =  .  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="32" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="30" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7498-7514" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  B/<?xml version="1.0"?><Primers recycledIDs="27,28,21,1,26,24,23,30,29,25,37,12" nextValidID="40"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="39" name="P819 PriM_mpk_R2" sequence="atatGGTACCtgtaaagccaaatatactttctcttcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7340-7366" boundStrand="1" annealedBases="tgtaaagccaaatatactttctcttcg" meltingTemperature="54"><Component bases="atatGGTACC"/><Component hybridizedRange="7340-7366" bases="tgtaaagccaaatatactttctcttcg"/></BindingSite><BindingSite simplified="1" location="7340-7366" boundStrand="1" annealedBases="tgtaaagccaaatatactttctcttcg" meltingTemperature="54"><Component bases="atatGGTACC"/><Component hybridizedRange="7340-7366" bases="tgtaaagccaaatatactttctcttcg"/></BindingSite></Primer><Primer recentID="38" name="P428 FTL0702_VSVG_2F" sequence="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGCttcttctttaggcaaaacattacttaag" description="0"><BindingSite location="7385-7412" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGC"/><Component hybridizedRange="7385-7412" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="7385-7412" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGC"/><Component hybridizedRange="7385-7412" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="36" name="P365 FTL0702cmp_R1" sequence="atatGCGGCCGCttcttctttaggcaaaacattacttaag" description="Contains NotI site"><BindingSite location="7385-7412" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="7385-7412" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="7385-7412" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="7385-7412" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="35" name="P433 FTL0702cmp_R2" sequence="atatGGATCCtcattcttctttaggcaaaacattactt" description="Contains BamHI site"><BindingSite location="7388-7415" boundStrand="1" annealedBases="tcattcttctttaggcaaaacattactt" meltingTemperature="55"><Component bases="atatGGATCC"/><Component hybridizedRange="7388-7415" bases="tcattcttctttaggcaaaacattactt"/></BindingSite><BindingSite simplified="1" location="7388-7415" boundStrand="1" annealedBases="tcattcttctttaggcaaaacattactt" meltingTemperature="55"><Component bases="atatGGATCC"/><Component hybridizedRange="7388-7415" bases="tcattcttctttaggcaaaacattactt"/></BindingSite></Primer><Primer recentID="34" name="P366 FTL0702cmp_R2" sequence="atatGGATCCttcttctttaggcaaaacattacttaag" description="&quot;Contains BamHI site, could use to make vector lacking VSVG tag&quot;"><BindingSite location="7385-7412" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGGATCC"/><Component hybridizedRange="7385-7412" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="7385-7412" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGGATCC"/><Component hybridizedRange="7385-7412" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="33" name="P702 PriM_mtip1_R2" sequence="atatGGTACCtactttctcttcgaaaacggtgtcg" description="Contains KpnI site"><BindingSite location="7328-7352" boundStrand="1" annealedBases="tactttctcttcgaaaacggtgtcg" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="7328-7352" bases="tactttctcttcgaaaacggtgtcg"/></BindingSite><BindingSite simplified="1" location="7328-7352" boundStrand="1" annealedBases="tactttctcttcgaaaacggtgtcg" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="7328-7352" bases="tactttctcttcgaaaacggtgtcg"/></BindingSite></Primer><Primer recentID="32" name="P438 RT_FTL0702-2R" sequence="agttgtccggctatctgtttag" description="0"><BindingSite location="7288-7309" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="7288-7309" bases="agttgtccggctatctgtttag"/></BindingSite><BindingSite simplified="1" location="7288-7309" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="7288-7309" bases="agttgtccggctatctgtttag"/></BindingSite></Primer><Primer recentID="2" name="KROL23 PriMnoC2FR2F" sequence="aaaaatgcaGCtaattttacagcagctaaaaatagtaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7218-7257" boundStrand="0" annealedBases="aaaaatgcaGCtaattttacagcagctaaaaatagtaaca" meltingTemperature="61"><Component hybridizedRange="7218-7257" bases="aaaaatgcaGCtaattttacagcagctaaaaatagtaaca"/></BindingSite><BindingSite simplified="1" location="7218-7257" boundStrand="0" annealedBases="aaaaatgcaGCtaattttacagcagctaaaaatagtaaca" meltingTemperature="61"><Component hybridizedRange="7218-7257" bases="aaaaatgcaGCtaattttacagcagctaaaaatagtaaca"/></BindingSite></Primer><Primer recentID="8" name="KROL24 PriMnoC2FR2R" sequence="atatGGTACCtgtcgaaagtgattcttagattcagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7445-7476" boundStrand="1" annealedBases="GGTACCtgtcgaaagtgattcttagattcagc" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="7445-7476" bases="GGTACCtgtcgaaagtgattcttagattcagc"/></BindingSite><BindingSite simplified="1" location="7445-7476" boundStrand="1" annealedBases="GGTACCtgtcgaaagtgattcttagattcagc" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="7445-7476" bases="GGTACCtgtcgaaagtgattcttagattcagc"/></BindingSite></Primer><Primer recentID="31" name="P821 PriM_mtip1_R3" sequence="atatGGTACCgtaactttggtattagttctgatactg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6881-6907" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6881-6907" bases="gtaactttggtattagttctgatactg"/></BindingSite><BindingSite simplified="1" location="6881-6907" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6881-6907" bases="gtaactttggtattagttctgatactg"/></BindingSite></Primer><Primer recentID="22" name="P444 pKL75_F" sequence="atatGGTACCagcttaatcagtatcagaactaatacc" description="Contains KpnI site"><BindingSite location="6873-6899" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6873-6899" bases="agcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6873-6899" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6873-6899" bases="agcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="0" name="P429 FTL0702_VSVG_2R" sequence="atatGAATTCagttgctgaacaagctaaactaaaaag" description="0"><BindingSite location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite><BindingSite simplified="1" location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite></Primer><Primer recentID="3" name="P411 FTL0702sp_2R" sequence="atatGGTACCgcatttgtagctgctttaagtgccc" description="Contains KpnI site"><BindingSite location="7036-7060" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="7036-7060" bases="gcatttgtagctgctttaagtgccc"/></BindingSite><BindingSite simplified="1" location="7036-7060" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="7036-7060" bases="gcatttgtagctgctttaagtgccc"/></BindingSite></Primer><Primer recentID="4" name="P368 FTL0702seqR1" sequence="acttgtagatattaacattaaagtatgac" description="0"><BindingSite location="7105-7133" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="7105-7133" bases="acttgtagatattaacattaaagtatgac"/></BindingSite><BindingSite simplified="1" location="7105-7133" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="7105-7133" bases="acttgtagatattaacattaaagtatgac"/></BindingSite></Primer><Primer recentID="5" name="P430 CkFTL0702V_R" sequence="gtcatactttaatgttaatatctacaagt" description="0"><BindingSite location="7105-7133" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="7105-7133" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite><BindingSite simplified="1" location="7105-7133" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="7105-7133" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite></Primer><Primer recentID="6" name="P704 CkPriM_mtip1R" sequence="aatatcattaaaatacgccttaggacc" description="0"><BindingSite location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite><BindingSite simplified="1" location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite></Primer><Primer recentID="7" name="P367 FTL0702seqF1" sequence="ctcaaggtcttgagaaatacagagc" description="0"><BindingSite location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite></Primer><Primer recentID="9" name="P413 CkFTL0702stp30R" sequence="gctctgtatttctcaagaccttgag" description="0"><BindingSite location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite></Primer><Primer recentID="10" name="P697 Ck_PriM_mpk1F" sequence="cagcatgggattctgctactagtc" description="0"><BindingSite location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite><BindingSite simplified="1" location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite></Primer><Primer recentID="11" name="P437 RT_FTL0702-2F" sequence="agcaagtaagtgatgctgataga" description="0"><BindingSite location="7183-7205" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="7183-7205" bases="agcaagtaagtgatgctgataga"/></BindingSite><BindingSite simplified="1" location="7183-7205" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="7183-7205" bases="agcaagtaagtgatgctgataga"/></BindingSite></Primer><Primer recentID="13" name="P439 RT_FTL0702-3F" sequence="tgctgatgctaacacacttaca" description="0"><BindingSite location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite><BindingSite simplified="1" location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite></Primer><Primer recentID="14" name="P436 RT_FTL0702-1R" sequence="cggcatctttgctattgatgtt" description="0"><BindingSite location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite><BindingSite simplified="1" location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite></Primer><Primer recentID="15" name="P440 RT_FTL0702-3R" sequence="ccgttcttggcgaagctatt" description="0"><BindingSite location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite><BindingSite simplified="1" location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite></Primer><Primer recentID="16" name="P435 RT_FTL0702-1F" sequence="cagcatgggattctgctact" description="0"><BindingSite location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite><BindingSite simplified="1" location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite></Primer><Primer recentID="17" name="KROL19 PriMnoCFR1F" sequence="atatGGATCCaatttatgcattcaaaagcatagcagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6101-6133" boundStrand="0" annealedBases="GGATCCaatttatgcattcaaaagcatagcagc" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="6101-6133" bases="GGATCCaatttatgcattcaaaagcatagcagc"/></BindingSite><BindingSite simplified="1" location="6101-6133" boundStrand="0" annealedBases="GGATCCaatttatgcattcaaaagcatagcagc" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="6101-6133" bases="GGATCCaatttatgcattcaaaagcatagcagc"/></BindingSite></Primer><Primer recentID="18" name="KROL20 PriMnoC1FR1R" sequence="aattgatagagccgttcttggcgaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6839-6863" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6839-6863" bases="aattgatagagccgttcttggcgaa"/></BindingSite><BindingSite simplified="1" location="6839-6863" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6839-6863" bases="aattgatagagccgttcttggcgaa"/></BindingSite></Primer><Primer recentID="19" name="KROL21 PriMnoC1FR2F" sequence="atatGCtgaagctagcttaatcagtatcagaactaatacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6866-6899" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="58"><Component bases="atatGC"/><Component hybridizedRange="6866-6899" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6866-6899" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="58"><Component bases="atatGC"/><Component hybridizedRange="6866-6899" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="20" name="KROL22 PriMnoC2FR1R" sequence="gtaaaattaGCtgcatttttaccaaatagaactctatca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7199-7237" boundStrand="1" annealedBases="gtaaaattaGCtgcatttttaccaaatagaactctatca" meltingTemperature="59"><Component hybridizedRange="7199-7237" bases="gtaaaattaGCtgcatttttaccaaatagaactctatca"/></BindingSite><BindingSite simplified="1" location="7199-7237" boundStrand="1" annealedBases="gtaaaattaGCtgcatttttaccaaatagaactctatca" meltingTemperature="59"><Component hybridizedRange="7199-7237" bases="gtaaaattaGCtgcatttttaccaaatagaactctatca"/></BindingSite></Primer></Primers>
  D<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="16:48:45">2018.12.17</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAjckU6M8cRYMXUB^]`)F|^UF׎;PY8TF]UTQZC$yU&yVFoBlUS8Ql*YUYX/2jmڕ69^bGhf+#Q$X-f]TcxqyyyiK$uM*f8cwftd1I"UVN\;ZSET9ityeU8~gwy&z~FV^zTӝX֑Bdrgbnu$(cmC8Eju5%!%V6%tӕY%9:tduBԦ|Rfu*zRX>TQayajZZNެt'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ    .  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ;<?xml version="1.0"?><Features nextValidID="47"><Feature recentID="46" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7239-7471" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="45" name="RPp7" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7407-7435" color="#0080ff" type="standard"/><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="44" name="P427 FTL0702_VSVG_1R" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7417-7446" color="#50ff50" type="standard"/><Q name="note"><V text="P427 FTL0702_VSVG_1R"/></Q></Feature><Feature recentID="43" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7239-7416" color="#00e600" type="standard"/></Feature><Feature recentID="42" name="hypothetical protein" directionality="1" translationMW="6318.53" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="428" hitsStopCodon="1"><Segment range="7239-7416" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="9" name="P365 FTL0702cmp_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7386-7413" color="#8080ff" type="standard"/><Q name="note"><V text="P365 FTL0702cmp_R"/></Q></Feature><Feature recentID="8" name="P428 FTL0702_VSVG_2F" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7386-7413" color="#50ff50" type="standard"/><Q name="note"><V text="P428 FTL0702_VSVG_2F"/></Q></Feature><Feature recentID="7" name="P433 FTL0702cmp_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7389-7416" color="#50ff50" type="standard"/><Q name="note"><V text="P433 FTL0702cmp_R2"/></Q></Feature><Feature recentID="3" name="P438 RT_FTL0702-2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7289-7310" color="#50ff50" type="standard"/><Q name="note"><V text="P438 RT_FTL0702-2R"/></Q></Feature><Feature recentID="0" name="P702 PriM_mtip1_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7329-7353" color="#50ff50" type="standard"/><Q name="note"><V text="P702 PriM_mtip1_R2"/></Q></Feature><Feature recentID="41" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-7238" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="40" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-7238" color="#00e600" type="standard"/></Feature><Feature recentID="39" name="hypothetical protein" directionality="1" translationMW="41572.78" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="51" maxRunOn="7416" maxFusedRunOn="7416"><Segment range="6108-7238" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="25" name="P367 FTL0702seqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6266-6290" color="#8080ff" type="standard"/><Q name="note"><V text="P367 FTL0702seqF1"/></Q></Feature><Feature recentID="24" name="P368 FTL0702seqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7106-7134" color="#8080ff" type="standard"/><Q name="note"><V text="P368 FTL0702seqR1"/></Q></Feature><Feature recentID="23" name="P411 FTL0702sp_2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7037-7061" color="#50ff50" type="standard"/><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="22" name="P429 FTL0702_VSVG_2R" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6363-6389" color="#50ff50" type="standard"/><Q name="note"><V text="P429 FTL0702_VSVG_2R"/></Q></Feature><Feature recentID="21" name="P435 RT_FTL0702-1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6551-6570" color="#50ff50" type="standard"/><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="20" name="P436 RT_FTL0702-1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6649-6670" color="#50ff50" type="standard"/><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="19" name="P437 RT_FTL0702-2F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7184-7206" color="#50ff50" type="standard"/><Q name="note"><V text="P437 RT_FTL0702-2F"/></Q></Feature><Feature recentID="18" name="P439 RT_FTL0702-3F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6768-6789" color="#50ff50" type="standard"/><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="17" name="P440 RT_FTL0702-3R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6834-6853" color="#50ff50" type="standard"/><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="16" name="P444 pKL75_F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6874-6900" color="#50ff50" type="standard"/><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="15" name="P692 PriM_mpk_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6586-6612" color="#50ff50" type="standard"/><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="14" name="P693 PriM_mpk_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6637-6665" color="#50ff50" type="standard"/><Q name="note"><V text="P693 PriM_mpk_F2"/></Q></Feature><Feature recentID="13" name="P698 Ck_PriM_mpk1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7027-7049" color="#50ff50" type="standard"/><Q name="note"><V text="P698 Ck_PriM_mpk1R"/></Q></Feature><Feature recentID="12" name="P697 Ck_PriM_mpk1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6551-6574" color="#50ff50" type="standard"/><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature><Feature recentID="11" name="P700 PriM_mtip1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6291-6318" color="#50ff50" type="standard"/><Q name="note"><V text="P700 PriM_mtip1_R1"/></Q></Feature><Feature recentID="10" name="P701 PriM_mtip1_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6343-6368" color="#50ff50" type="standard"/><Q name="note"><V text="P701 PriM_mtip1_F2"/></Q></Feature><Feature recentID="6" name="P704 CkPriM_mtip1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6496-6522" color="#50ff50" type="standard"/><Q name="note"><V text="P704 CkPriM_mtip1R"/></Q></Feature><Feature recentID="5" name="P706 PriM_mtip2_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6291-6318" color="#50ff50" type="standard"/><Q name="note"><V text="P706 PriM_mtip2_R1"/></Q></Feature><Feature recentID="4" name="P705 PriM_mtip2_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6358-6384" color="#50ff50" type="standard"/><Q name="note"><V text="P705 PriM_mtip2_F2"/></Q></Feature><Feature recentID="2" name="P707 PriM_unst1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6579-6605" color="#50ff50" type="standard"/><Q name="note"><V text="P707 PriM_unst1_R1"/></Q></Feature><Feature recentID="1" name="P708 PriM_unst1_F1" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6616-6640" color="#50ff50" type="standard"/><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature><Feature recentID="38" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8200" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="37" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7472-7476" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="36" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7906-288" color="#ffff00" type="standard"/><Q name="note"><V text="ColE origin"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="35" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="34" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="32" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="30" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7498-7514" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  h<?xml version="1.0"?><Primers nextValidID="40"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="39" name="P819 PriM_mpk_R2" sequence="atatGGTACCtgtaaagccaaatatactttctcttcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7340-7366" boundStrand="1" annealedBases="tgtaaagccaaatatactttctcttcg" meltingTemperature="54"><Component bases="atatGGTACC"/><Component hybridizedRange="7340-7366" bases="tgtaaagccaaatatactttctcttcg"/></BindingSite><BindingSite simplified="1" location="7340-7366" boundStrand="1" annealedBases="tgtaaagccaaatatactttctcttcg" meltingTemperature="54"><Component bases="atatGGTACC"/><Component hybridizedRange="7340-7366" bases="tgtaaagccaaatatactttctcttcg"/></BindingSite></Primer><Primer recentID="38" name="P428 FTL0702_VSVG_2F" sequence="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGCttcttctttaggcaaaacattacttaag" description="0"><BindingSite location="7385-7412" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGC"/><Component hybridizedRange="7385-7412" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="7385-7412" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGC"/><Component hybridizedRange="7385-7412" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="37" name="P427 FTL0702_VSVG_1R" sequence="GATATTGAAATGAATAGATTAGGTAAATAAactaatgactaaatttctaaaattaattag" description="0"><BindingSite location="7415-7445" boundStrand="0" annealedBases="Aactaatgactaaatttctaaaattaattag" meltingTemperature="51"><Component bases="GATATTGAAATGAATAGATTAGGTAAATA"/><Component hybridizedRange="7415-7445" bases="Aactaatgactaaatttctaaaattaattag"/></BindingSite><BindingSite simplified="1" location="7415-7445" boundStrand="0" annealedBases="Aactaatgactaaatttctaaaattaattag" meltingTemperature="51"><Component bases="GATATTGAAATGAATAGATTAGGTAAATA"/><Component hybridizedRange="7415-7445" bases="Aactaatgactaaatttctaaaattaattag"/></BindingSite></Primer><Primer recentID="36" name="P365 FTL0702cmp_R1" sequence="atatGCGGCCGCttcttctttaggcaaaacattacttaag" description="Contains NotI site"><BindingSite location="7385-7412" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="7385-7412" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="7385-7412" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="7385-7412" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="35" name="P433 FTL0702cmp_R2" sequence="atatGGATCCtcattcttctttaggcaaaacattactt" description="Contains BamHI site"><BindingSite location="7388-7415" boundStrand="1" annealedBases="tcattcttctttaggcaaaacattactt" meltingTemperature="55"><Component bases="atatGGATCC"/><Component hybridizedRange="7388-7415" bases="tcattcttctttaggcaaaacattactt"/></BindingSite><BindingSite simplified="1" location="7388-7415" boundStrand="1" annealedBases="tcattcttctttaggcaaaacattactt" meltingTemperature="55"><Component bases="atatGGATCC"/><Component hybridizedRange="7388-7415" bases="tcattcttctttaggcaaaacattactt"/></BindingSite></Primer><Primer recentID="34" name="P366 FTL0702cmp_R2" sequence="atatGGATCCttcttctttaggcaaaacattacttaag" description="&quot;Contains BamHI site, could use to make vector lacking VSVG tag&quot;"><BindingSite location="7385-7412" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGGATCC"/><Component hybridizedRange="7385-7412" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="7385-7412" boundStrand="1" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="53"><Component bases="atatGGATCC"/><Component hybridizedRange="7385-7412" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="33" name="P702 PriM_mtip1_R2" sequence="atatGGTACCtactttctcttcgaaaacggtgtcg" description="Contains KpnI site"><BindingSite location="7328-7352" boundStrand="1" annealedBases="tactttctcttcgaaaacggtgtcg" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="7328-7352" bases="tactttctcttcgaaaacggtgtcg"/></BindingSite><BindingSite simplified="1" location="7328-7352" boundStrand="1" annealedBases="tactttctcttcgaaaacggtgtcg" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="7328-7352" bases="tactttctcttcgaaaacggtgtcg"/></BindingSite></Primer><Primer recentID="32" name="P438 RT_FTL0702-2R" sequence="agttgtccggctatctgtttag" description="0"><BindingSite location="7288-7309" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="7288-7309" bases="agttgtccggctatctgtttag"/></BindingSite><BindingSite simplified="1" location="7288-7309" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="7288-7309" bases="agttgtccggctatctgtttag"/></BindingSite></Primer><Primer recentID="2" name="KROL23 PriMnoC2FR2F" sequence="aaaaatgcaGCtaattttacagcagctaaaaatagtaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7218-7257" boundStrand="0" annealedBases="aaaaatgcataattttacagcagctaaaaatagtaaca" meltingTemperature="54"><Component hybridizedRange="7218-7226" bases="aaaaatgca"/><Component bases="GC"/><Component hybridizedRange="7229-7257" bases="taattttacagcagctaaaaatagtaaca"/></BindingSite><BindingSite simplified="1" location="7218-7257" boundStrand="0" annealedBases="aaaaatgcataattttacagcagctaaaaatagtaaca" meltingTemperature="54"><Component hybridizedRange="7218-7226" bases="aaaaatgca"/><Component bases="GC"/><Component hybridizedRange="7229-7257" bases="taattttacagcagctaaaaatagtaaca"/></BindingSite></Primer><Primer recentID="8" name="KROL24 PriMnoC2FR2R" sequence="atatGGTACCtgtcgaaagtgattcttagattcagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7445-7476" boundStrand="1" annealedBases="GGTACCtgtcgaaagtgattcttagattcagc" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="7445-7476" bases="GGTACCtgtcgaaagtgattcttagattcagc"/></BindingSite><BindingSite simplified="1" location="7445-7476" boundStrand="1" annealedBases="GGTACCtgtcgaaagtgattcttagattcagc" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="7445-7476" bases="GGTACCtgtcgaaagtgattcttagattcagc"/></BindingSite></Primer><Primer recentID="31" name="P821 PriM_mtip1_R3" sequence="atatGGTACCgtaactttggtattagttctgatactg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6881-6907" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6881-6907" bases="gtaactttggtattagttctgatactg"/></BindingSite><BindingSite simplified="1" location="6881-6907" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6881-6907" bases="gtaactttggtattagttctgatactg"/></BindingSite></Primer><Primer recentID="30" name="P707 PriM_unst1_R1" sequence="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6578-6624" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6615-6624" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6606-6611" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6578-6604" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite><BindingSite simplified="1" location="6578-6624" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6615-6624" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6606-6611" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6578-6604" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite></Primer><Primer recentID="29" name="P708 PriM_unst1_F1" sequence="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6595-6639" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6595-6604" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6606-6611" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6615-6639" bases="ggtgcaagagtagttctttggaatg"/></BindingSite><BindingSite simplified="1" location="6595-6639" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6595-6604" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6606-6611" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6615-6639" bases="ggtgcaagagtagttctttggaatg"/></BindingSite></Primer><Primer recentID="28" name="P705 PriM_mtip2_R1" sequence="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="6290-6317" boundStrand="1" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="6290-6317" boundStrand="1" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="27" name="P706 PriM_mtip2_F2" sequence="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGGTaaagtagttgctgaacaagctaaacta" description="0"><BindingSite location="6345-6383" boundStrand="0" annealedBases="GGCGGTaaagtagttgctgaacaagctaaacta" meltingTemperature="52"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGT"/><Component hybridizedRange="6345-6347" bases="GGC"/><Component hybridizedRange="6349-6350" bases="GG"/><Component hybridizedRange="6356-6383" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite><BindingSite simplified="1" location="6356-6383" boundStrand="0" annealedBases="Taaagtagttgctgaacaagctaaacta" meltingTemperature="52"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGG"/><Component hybridizedRange="6356-6383" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite></Primer><Primer recentID="26" name="P700 PriM_mtip1_R1" sequence="ctaaTTCTTCttgactttcaagCTCTTCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="6290-6340" boundStrand="1" annealedBases="TTCTTttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaa"/><Component hybridizedRange="6336-6340" bases="TTCTT"/><Component bases="C"/><Component hybridizedRange="6324-6335" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="6290-6335" boundStrand="1" annealedBases="ttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaaTTCTTC"/><Component hybridizedRange="6324-6335" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="25" name="P693 PriM_mpk_F2" sequence="GCAggtgcaGCAgtagttcttGCAaatggtcaatataacatcaatagcaaaga" description="0"><BindingSite location="6615-6664" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6615-6620" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6623-6632" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6636-6664" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite><BindingSite simplified="1" location="6615-6664" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6615-6620" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6623-6632" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6636-6664" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite></Primer><Primer recentID="24" name="P701 PriM_mtip1_F2" sequence="atcGAAGAGcttgaaagtcaaGAAGAAttaggctggagaattaaagtagttgc" description="0"><BindingSite location="6324-6367" boundStrand="0" annealedBases="cttgaaagtcaaAAGAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="6324-6335" bases="cttgaaagtcaa"/><Component bases="G"/><Component hybridizedRange="6336-6339" bases="AAGA"/><Component hybridizedRange="6341-6367" bases="Attaggctggagaattaaagtagttgc"/></BindingSite><BindingSite simplified="1" location="6324-6367" boundStrand="0" annealedBases="cttgaaagtcaaAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="6324-6335" bases="cttgaaagtcaa"/><Component bases="GAAG"/><Component hybridizedRange="6340-6367" bases="AAttaggctggagaattaaagtagttgc"/></BindingSite></Primer><Primer recentID="23" name="P692 PriM_mpk_R1" sequence="TGCaagaactacTGCtgcaccTGCgaacatatcaatgttgcttctgtagtc" description="0"><BindingSite location="6585-6632" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6623-6632" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6615-6620" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6585-6611" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite><BindingSite simplified="1" location="6585-6632" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6623-6632" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6615-6620" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6585-6611" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite></Primer><Primer recentID="22" name="P444 pKL75_F" sequence="atatGGTACCagcttaatcagtatcagaactaatacc" description="Contains KpnI site"><BindingSite location="6873-6899" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6873-6899" bases="agcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6873-6899" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6873-6899" bases="agcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="21" name="P695 PriM_mpk_R3" sequence="catcagcTGCtagtgaaactgctttagttgtatcact" description="0"><BindingSite location="6738-6774" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6768-6774" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6738-6764" bases="tagtgaaactgctttagttgtatcact"/></BindingSite><BindingSite simplified="1" location="6738-6774" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6768-6774" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6738-6764" bases="tagtgaaactgctttagttgtatcact"/></BindingSite></Primer><Primer recentID="0" name="P429 FTL0702_VSVG_2R" sequence="atatGAATTCagttgctgaacaagctaaactaaaaag" description="0"><BindingSite location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite><BindingSite simplified="1" location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite></Primer><Primer recentID="1" name="P696 PriM_mpk_F4" sequence="ttcactaGCAgctgatgctaacacacttacatatac" description="0"><BindingSite location="6768-6793" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6768-6793" bases="gctgatgctaacacacttacatatac"/></BindingSite><BindingSite simplified="1" location="6768-6793" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6768-6793" bases="gctgatgctaacacacttacatatac"/></BindingSite></Primer><Primer recentID="3" name="P411 FTL0702sp_2R" sequence="atatGGTACCgcatttgtagctgctttaagtgccc" description="Contains KpnI site"><BindingSite location="7036-7060" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="7036-7060" bases="gcatttgtagctgctttaagtgccc"/></BindingSite><BindingSite simplified="1" location="7036-7060" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="7036-7060" bases="gcatttgtagctgctttaagtgccc"/></BindingSite></Primer><Primer recentID="4" name="P368 FTL0702seqR1" sequence="acttgtagatattaacattaaagtatgac" description="0"><BindingSite location="7105-7133" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="7105-7133" bases="acttgtagatattaacattaaagtatgac"/></BindingSite><BindingSite simplified="1" location="7105-7133" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="7105-7133" bases="acttgtagatattaacattaaagtatgac"/></BindingSite></Primer><Primer recentID="5" name="P430 CkFTL0702V_R" sequence="gtcatactttaatgttaatatctacaagt" description="0"><BindingSite location="7105-7133" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="7105-7133" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite><BindingSite simplified="1" location="7105-7133" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="7105-7133" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite></Primer><Primer recentID="6" name="P704 CkPriM_mtip1R" sequence="aatatcattaaaatacgccttaggacc" description="0"><BindingSite location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite><BindingSite simplified="1" location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite></Primer><Primer recentID="7" name="P367 FTL0702seqF1" sequence="ctcaaggtcttgagaaatacagagc" description="0"><BindingSite location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite></Primer><Primer recentID="9" name="P413 CkFTL0702stp30R" sequence="gctctgtatttctcaagaccttgag" description="0"><BindingSite location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite></Primer><Primer recentID="10" name="P697 Ck_PriM_mpk1F" sequence="cagcatgggattctgctactagtc" description="0"><BindingSite location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite><BindingSite simplified="1" location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite></Primer><Primer recentID="11" name="P437 RT_FTL0702-2F" sequence="agcaagtaagtgatgctgataga" description="0"><BindingSite location="7183-7205" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="7183-7205" bases="agcaagtaagtgatgctgataga"/></BindingSite><BindingSite simplified="1" location="7183-7205" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="7183-7205" bases="agcaagtaagtgatgctgataga"/></BindingSite></Primer><Primer recentID="12" name="P698 Ck_PriM_mpk1R" sequence="gctttaagtgcccagttgtcgcc" description="0"><BindingSite location="7026-7048" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="7026-7048" bases="gctttaagtgcccagttgtcgcc"/></BindingSite><BindingSite simplified="1" location="7026-7048" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="7026-7048" bases="gctttaagtgcccagttgtcgcc"/></BindingSite></Primer><Primer recentID="13" name="P439 RT_FTL0702-3F" sequence="tgctgatgctaacacacttaca" description="0"><BindingSite location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite><BindingSite simplified="1" location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite></Primer><Primer recentID="14" name="P436 RT_FTL0702-1R" sequence="cggcatctttgctattgatgtt" description="0"><BindingSite location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite><BindingSite simplified="1" location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite></Primer><Primer recentID="15" name="P440 RT_FTL0702-3R" sequence="ccgttcttggcgaagctatt" description="0"><BindingSite location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite><BindingSite simplified="1" location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite></Primer><Primer recentID="16" name="P435 RT_FTL0702-1F" sequence="cagcatgggattctgctact" description="0"><BindingSite location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite><BindingSite simplified="1" location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite></Primer><Primer recentID="17" name="KROL19 PriMnoCFR1F" sequence="aatttatgcattcaaaagcatagcagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6107-6133" boundStrand="0" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="6107-6133" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite><BindingSite simplified="1" location="6107-6133" boundStrand="0" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="6107-6133" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite></Primer><Primer recentID="18" name="KROL20 PriMnoC1FR1R" sequence="aattgatagagccgttcttggcgaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6839-6863" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6839-6863" bases="aattgatagagccgttcttggcgaa"/></BindingSite><BindingSite simplified="1" location="6839-6863" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6839-6863" bases="aattgatagagccgttcttggcgaa"/></BindingSite></Primer><Primer recentID="19" name="KROL21 PriMnoC1FR2F" sequence="atatGCtgaagctagcttaatcagtatcagaactaatacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6866-6899" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="58"><Component bases="atatGC"/><Component hybridizedRange="6866-6899" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6866-6899" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="58"><Component bases="atatGC"/><Component hybridizedRange="6866-6899" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="20" name="KROL22 PriMnoC2FR1R" sequence="gtaaaattaGCtgcatttttaccaaatagaactctatca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7199-7237" boundStrand="1" annealedBases="gtaaaattatgcatttttaccaaatagaactctatca" meltingTemperature="51"><Component hybridizedRange="7229-7237" bases="gtaaaatta"/><Component bases="GC"/><Component hybridizedRange="7199-7226" bases="tgcatttttaccaaatagaactctatca"/></BindingSite><BindingSite simplified="1" location="7199-7237" boundStrand="1" annealedBases="gtaaaattatgcatttttaccaaatagaactctatca" meltingTemperature="51"><Component hybridizedRange="7229-7237" bases="gtaaaatta"/><Component bases="GC"/><Component hybridizedRange="7199-7226" bases="tgcatttttaccaaatagaactctatca"/></BindingSite></Primer></Primers>
  D<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="16:41:54">2018.12.17</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                 .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
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ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAjckU6M8cRYMXUB^]`)F|^UF׎;PY8TF]UTQZC$yU&yVFoBlUS8Ql*YUYX/2jmڕ69^bGhf+#Q$X-f]TcxqyyyiK$uM*f8cwftd1I"UVN\;ZSET9ityeU8~gwy&z~FV^zTӝX֑Bdrgbnu$(cmC8Eju5%!%V6%pk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>    E  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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text="LEFT"/></Q></Feature><Feature recentID="35" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="34" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="32" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="30" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7265-7281" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  N<?xml version="1.0"?><Primers recycledIDs="8,2" nextValidID="32"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="31" name="P821 PriM_mtip1_R3" sequence="atatGGTACCgtaactttggtattagttctgatactg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6881-6907" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6881-6907" bases="gtaactttggtattagttctgatactg"/></BindingSite><BindingSite simplified="1" location="6881-6907" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6881-6907" bases="gtaactttggtattagttctgatactg"/></BindingSite></Primer><Primer recentID="30" name="P707 PriM_unst1_R1" sequence="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6578-6624" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6615-6624" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6606-6611" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6578-6604" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite><BindingSite simplified="1" location="6578-6624" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6615-6624" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6606-6611" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6578-6604" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite></Primer><Primer recentID="29" name="P708 PriM_unst1_F1" sequence="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6595-6639" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6595-6604" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6606-6611" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6615-6639" bases="ggtgcaagagtagttctttggaatg"/></BindingSite><BindingSite simplified="1" location="6595-6639" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6595-6604" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6606-6611" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6615-6639" bases="ggtgcaagagtagttctttggaatg"/></BindingSite></Primer><Primer recentID="28" name="P705 PriM_mtip2_R1" sequence="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="6290-6317" boundStrand="1" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="6290-6317" boundStrand="1" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="27" name="P706 PriM_mtip2_F2" sequence="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGGTaaagtagttgctgaacaagctaaacta" description="0"><BindingSite location="6345-6383" boundStrand="0" annealedBases="GGCGGTaaagtagttgctgaacaagctaaacta" meltingTemperature="52"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGT"/><Component hybridizedRange="6345-6347" bases="GGC"/><Component hybridizedRange="6349-6350" bases="GG"/><Component hybridizedRange="6356-6383" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite><BindingSite simplified="1" location="6356-6383" boundStrand="0" annealedBases="Taaagtagttgctgaacaagctaaacta" meltingTemperature="52"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGG"/><Component hybridizedRange="6356-6383" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite></Primer><Primer recentID="26" name="P700 PriM_mtip1_R1" sequence="ctaaTTCTTCttgactttcaagCTCTTCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="6290-6340" boundStrand="1" annealedBases="TTCTTttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaa"/><Component hybridizedRange="6336-6340" bases="TTCTT"/><Component bases="C"/><Component hybridizedRange="6324-6335" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="6290-6335" boundStrand="1" annealedBases="ttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaaTTCTTC"/><Component hybridizedRange="6324-6335" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="25" name="P693 PriM_mpk_F2" sequence="GCAggtgcaGCAgtagttcttGCAaatggtcaatataacatcaatagcaaaga" description="0"><BindingSite location="6615-6664" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6615-6620" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6623-6632" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6636-6664" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite><BindingSite simplified="1" location="6615-6664" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6615-6620" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6623-6632" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6636-6664" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite></Primer><Primer recentID="24" name="P701 PriM_mtip1_F2" sequence="atcGAAGAGcttgaaagtcaaGAAGAAttaggctggagaattaaagtagttgc" description="0"><BindingSite location="6324-6367" boundStrand="0" annealedBases="cttgaaagtcaaAAGAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="6324-6335" bases="cttgaaagtcaa"/><Component bases="G"/><Component hybridizedRange="6336-6339" bases="AAGA"/><Component hybridizedRange="6341-6367" bases="Attaggctggagaattaaagtagttgc"/></BindingSite><BindingSite simplified="1" location="6324-6367" boundStrand="0" annealedBases="cttgaaagtcaaAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="6324-6335" bases="cttgaaagtcaa"/><Component bases="GAAG"/><Component hybridizedRange="6340-6367" bases="AAttaggctggagaattaaagtagttgc"/></BindingSite></Primer><Primer recentID="23" name="P692 PriM_mpk_R1" sequence="TGCaagaactacTGCtgcaccTGCgaacatatcaatgttgcttctgtagtc" description="0"><BindingSite location="6585-6632" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6623-6632" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6615-6620" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6585-6611" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite><BindingSite simplified="1" location="6585-6632" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6623-6632" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6615-6620" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6585-6611" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite></Primer><Primer recentID="22" name="P444 pKL75_F" sequence="atatGGTACCagcttaatcagtatcagaactaatacc" description="Contains KpnI site"><BindingSite location="6873-6899" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6873-6899" bases="agcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6873-6899" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6873-6899" bases="agcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="21" name="P695 PriM_mpk_R3" sequence="catcagcTGCtagtgaaactgctttagttgtatcact" description="0"><BindingSite location="6738-6774" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6768-6774" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6738-6764" bases="tagtgaaactgctttagttgtatcact"/></BindingSite><BindingSite simplified="1" location="6738-6774" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6768-6774" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6738-6764" bases="tagtgaaactgctttagttgtatcact"/></BindingSite></Primer><Primer recentID="0" name="P429 FTL0702_VSVG_2R" sequence="atatGAATTCagttgctgaacaagctaaactaaaaag" description="0"><BindingSite location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite><BindingSite simplified="1" location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite></Primer><Primer recentID="1" name="P696 PriM_mpk_F4" sequence="ttcactaGCAgctgatgctaacacacttacatatac" description="0"><BindingSite location="6768-6793" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6768-6793" bases="gctgatgctaacacacttacatatac"/></BindingSite><BindingSite simplified="1" location="6768-6793" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6768-6793" bases="gctgatgctaacacacttacatatac"/></BindingSite></Primer><Primer recentID="3" name="P411 FTL0702sp_2R" sequence="atatGGTACCgcatttgtagctgctttaagtgccc" description="Contains KpnI site"><BindingSite location="7036-7060" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="7036-7060" bases="gcatttgtagctgctttaagtgccc"/></BindingSite><BindingSite simplified="1" location="7036-7060" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="7036-7060" bases="gcatttgtagctgctttaagtgccc"/></BindingSite></Primer><Primer recentID="4" name="P368 FTL0702seqR1" sequence="acttgtagatattaacattaaagtatgac" description="0"><BindingSite location="7105-7133" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="7105-7133" bases="acttgtagatattaacattaaagtatgac"/></BindingSite><BindingSite simplified="1" location="7105-7133" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="7105-7133" bases="acttgtagatattaacattaaagtatgac"/></BindingSite></Primer><Primer recentID="5" name="P430 CkFTL0702V_R" sequence="gtcatactttaatgttaatatctacaagt" description="0"><BindingSite location="7105-7133" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="7105-7133" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite><BindingSite simplified="1" location="7105-7133" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="7105-7133" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite></Primer><Primer recentID="6" name="P704 CkPriM_mtip1R" sequence="aatatcattaaaatacgccttaggacc" description="0"><BindingSite location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite><BindingSite simplified="1" location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite></Primer><Primer recentID="7" name="P367 FTL0702seqF1" sequence="ctcaaggtcttgagaaatacagagc" description="0"><BindingSite location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite></Primer><Primer recentID="9" name="P413 CkFTL0702stp30R" sequence="gctctgtatttctcaagaccttgag" description="0"><BindingSite location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite></Primer><Primer recentID="10" name="P697 Ck_PriM_mpk1F" sequence="cagcatgggattctgctactagtc" description="0"><BindingSite location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite><BindingSite simplified="1" location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite></Primer><Primer recentID="11" name="P437 RT_FTL0702-2F" sequence="agcaagtaagtgatgctgataga" description="0"><BindingSite location="7183-7205" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="7183-7205" bases="agcaagtaagtgatgctgataga"/></BindingSite><BindingSite simplified="1" location="7183-7205" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="7183-7205" bases="agcaagtaagtgatgctgataga"/></BindingSite></Primer><Primer recentID="12" name="P698 Ck_PriM_mpk1R" sequence="gctttaagtgcccagttgtcgcc" description="0"><BindingSite location="7026-7048" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="7026-7048" bases="gctttaagtgcccagttgtcgcc"/></BindingSite><BindingSite simplified="1" location="7026-7048" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="7026-7048" bases="gctttaagtgcccagttgtcgcc"/></BindingSite></Primer><Primer recentID="13" name="P439 RT_FTL0702-3F" sequence="tgctgatgctaacacacttaca" description="0"><BindingSite location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite><BindingSite simplified="1" location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite></Primer><Primer recentID="14" name="P436 RT_FTL0702-1R" sequence="cggcatctttgctattgatgtt" description="0"><BindingSite location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite><BindingSite simplified="1" location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite></Primer><Primer recentID="15" name="P440 RT_FTL0702-3R" sequence="ccgttcttggcgaagctatt" description="0"><BindingSite location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite><BindingSite simplified="1" location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite></Primer><Primer recentID="16" name="P435 RT_FTL0702-1F" sequence="cagcatgggattctgctact" description="0"><BindingSite location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite><BindingSite simplified="1" location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite></Primer><Primer recentID="17" name="KROL19 PriMnoCFR1F" sequence="aatttatgcattcaaaagcatagcagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6107-6133" boundStrand="0" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="6107-6133" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite><BindingSite simplified="1" location="6107-6133" boundStrand="0" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="6107-6133" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite></Primer><Primer recentID="18" name="KROL20 PriMnoC1FR1R" sequence="aattgatagagccgttcttggcgaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6839-6863" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6839-6863" bases="aattgatagagccgttcttggcgaa"/></BindingSite><BindingSite simplified="1" location="6839-6863" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6839-6863" bases="aattgatagagccgttcttggcgaa"/></BindingSite></Primer><Primer recentID="19" name="KROL21 PriMnoC1FR2F" sequence="atatGCtgaagctagcttaatcagtatcagaactaatacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6866-6899" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="58"><Component bases="atatGC"/><Component hybridizedRange="6866-6899" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6866-6899" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="58"><Component bases="atatGC"/><Component hybridizedRange="6866-6899" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="20" name="KROL22 PriMnoC2FR1R" sequence="gtaaaattaGCtgcatttttaccaaatagaactctatca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7199-7237" boundStrand="1" annealedBases="gtaaaattatgcatttttaccaaatagaactctatca" meltingTemperature="50"><Component hybridizedRange="7229-7237" bases="gtaaaatta"/><Component bases="GC"/><Component hybridizedRange="7199-7226" bases="tgcatttttaccaaatagaactctatca"/></BindingSite><BindingSite simplified="1" location="7199-7237" boundStrand="1" annealedBases="gtaaaattatgcatttttaccaaatagaactctatca" meltingTemperature="50"><Component hybridizedRange="7229-7237" bases="gtaaaatta"/><Component bases="GC"/><Component hybridizedRange="7199-7226" bases="tgcatttttaccaaatagaactctatca"/></BindingSite></Primer></Primers>
  D<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="16:41:50">2018.12.17</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	      !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  6<?xml version="1.0"?><Features nextValidID="42"><Feature recentID="41" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-7238" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="40" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-7238" color="#00e600" type="standard"/></Feature><Feature recentID="39" name="hypothetical protein" directionality="1" translationMW="41572.78" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="51" maxRunOn="7386" maxFusedRunOn="7386"><Segment range="6108-7238" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="25" name="P367 FTL0702seqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6266-6290" color="#8080ff" type="standard"/><Q name="note"><V text="P367 FTL0702seqF1"/></Q></Feature><Feature recentID="24" name="P368 FTL0702seqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7106-7134" color="#8080ff" type="standard"/><Q name="note"><V text="P368 FTL0702seqR1"/></Q></Feature><Feature recentID="23" name="P411 FTL0702sp_2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7037-7061" color="#50ff50" type="standard"/><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="22" name="P429 FTL0702_VSVG_2R" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6363-6389" color="#50ff50" type="standard"/><Q name="note"><V text="P429 FTL0702_VSVG_2R"/></Q></Feature><Feature recentID="21" name="P435 RT_FTL0702-1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6551-6570" color="#50ff50" type="standard"/><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="20" name="P436 RT_FTL0702-1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6649-6670" color="#50ff50" type="standard"/><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="19" name="P437 RT_FTL0702-2F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7184-7206" color="#50ff50" type="standard"/><Q name="note"><V text="P437 RT_FTL0702-2F"/></Q></Feature><Feature recentID="18" name="P439 RT_FTL0702-3F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6768-6789" color="#50ff50" type="standard"/><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="17" name="P440 RT_FTL0702-3R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6834-6853" color="#50ff50" type="standard"/><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="16" name="P444 pKL75_F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6874-6900" color="#50ff50" type="standard"/><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="15" name="P692 PriM_mpk_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6586-6612" color="#50ff50" type="standard"/><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="14" name="P693 PriM_mpk_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6637-6665" color="#50ff50" type="standard"/><Q name="note"><V text="P693 PriM_mpk_F2"/></Q></Feature><Feature recentID="13" name="P698 Ck_PriM_mpk1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7027-7049" color="#50ff50" type="standard"/><Q name="note"><V text="P698 Ck_PriM_mpk1R"/></Q></Feature><Feature recentID="12" name="P697 Ck_PriM_mpk1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6551-6574" color="#50ff50" type="standard"/><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature><Feature recentID="11" name="P700 PriM_mtip1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6291-6318" color="#50ff50" type="standard"/><Q name="note"><V text="P700 PriM_mtip1_R1"/></Q></Feature><Feature recentID="10" name="P701 PriM_mtip1_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6343-6368" color="#50ff50" type="standard"/><Q name="note"><V text="P701 PriM_mtip1_F2"/></Q></Feature><Feature recentID="6" name="P704 CkPriM_mtip1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6496-6522" color="#50ff50" type="standard"/><Q name="note"><V text="P704 CkPriM_mtip1R"/></Q></Feature><Feature recentID="5" name="P706 PriM_mtip2_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6291-6318" color="#50ff50" type="standard"/><Q name="note"><V text="P706 PriM_mtip2_R1"/></Q></Feature><Feature recentID="4" name="P705 PriM_mtip2_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6358-6384" color="#50ff50" type="standard"/><Q name="note"><V text="P705 PriM_mtip2_F2"/></Q></Feature><Feature recentID="2" name="P707 PriM_unst1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6579-6605" color="#50ff50" type="standard"/><Q name="note"><V text="P707 PriM_unst1_R1"/></Q></Feature><Feature recentID="1" name="P708 PriM_unst1_F1" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6616-6640" color="#50ff50" type="standard"/><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature><Feature recentID="38" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8082" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="37" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7354-7358" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="36" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7788-288" color="#ffff00" type="standard"/><Q name="note"><V text="ColE origin"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="35" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="34" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="32" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="30" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7380-7396" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7239-7353" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="8" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7239-7353" color="#00e600" type="standard"/></Feature><Feature recentID="7" name="hypothetical protein" directionality="1" translationMW="4009.83" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="428" maxRunOn="7386" maxFusedRunOn="7386"><Segment range="7239-7353" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P438 RT_FTL0702-2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7289-7310" color="#50ff50" type="standard"/><Q name="note"><V text="P438 RT_FTL0702-2R"/></Q></Feature><Feature recentID="0" name="P702 PriM_mtip1_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7329-7353" color="#50ff50" type="standard"/><Q name="note"><V text="P702 PriM_mtip1_R2"/></Q></Feature></Features>
  Sw<?xml version="1.0"?><Primers nextValidID="32"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="31" name="P821 PriM_mtip1_R3" sequence="atatGGTACCgtaactttggtattagttctgatactg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6881-6907" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6881-6907" bases="gtaactttggtattagttctgatactg"/></BindingSite><BindingSite simplified="1" location="6881-6907" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6881-6907" bases="gtaactttggtattagttctgatactg"/></BindingSite></Primer><Primer recentID="30" name="P707 PriM_unst1_R1" sequence="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6578-6624" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6615-6624" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6606-6611" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6578-6604" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite><BindingSite simplified="1" location="6578-6624" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6615-6624" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6606-6611" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6578-6604" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite></Primer><Primer recentID="29" name="P708 PriM_unst1_F1" sequence="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6595-6639" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6595-6604" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6606-6611" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6615-6639" bases="ggtgcaagagtagttctttggaatg"/></BindingSite><BindingSite simplified="1" location="6595-6639" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6595-6604" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6606-6611" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6615-6639" bases="ggtgcaagagtagttctttggaatg"/></BindingSite></Primer><Primer recentID="28" name="P705 PriM_mtip2_R1" sequence="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="6290-6317" boundStrand="1" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="6290-6317" boundStrand="1" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="27" name="P706 PriM_mtip2_F2" sequence="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGGTaaagtagttgctgaacaagctaaacta" description="0"><BindingSite location="6345-6383" boundStrand="0" annealedBases="GGCGGTaaagtagttgctgaacaagctaaacta" meltingTemperature="52"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGT"/><Component hybridizedRange="6345-6347" bases="GGC"/><Component hybridizedRange="6349-6350" bases="GG"/><Component hybridizedRange="6356-6383" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite><BindingSite simplified="1" location="6356-6383" boundStrand="0" annealedBases="Taaagtagttgctgaacaagctaaacta" meltingTemperature="52"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGG"/><Component hybridizedRange="6356-6383" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite></Primer><Primer recentID="26" name="P700 PriM_mtip1_R1" sequence="ctaaTTCTTCttgactttcaagCTCTTCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="6290-6340" boundStrand="1" annealedBases="TTCTTttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaa"/><Component hybridizedRange="6336-6340" bases="TTCTT"/><Component bases="C"/><Component hybridizedRange="6324-6335" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="6290-6335" boundStrand="1" annealedBases="ttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaaTTCTTC"/><Component hybridizedRange="6324-6335" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="25" name="P693 PriM_mpk_F2" sequence="GCAggtgcaGCAgtagttcttGCAaatggtcaatataacatcaatagcaaaga" description="0"><BindingSite location="6615-6664" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6615-6620" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6623-6632" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6636-6664" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite><BindingSite simplified="1" location="6615-6664" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6615-6620" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6623-6632" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6636-6664" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite></Primer><Primer recentID="24" name="P701 PriM_mtip1_F2" sequence="atcGAAGAGcttgaaagtcaaGAAGAAttaggctggagaattaaagtagttgc" description="0"><BindingSite location="6324-6367" boundStrand="0" annealedBases="cttgaaagtcaaAAGAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="6324-6335" bases="cttgaaagtcaa"/><Component bases="G"/><Component hybridizedRange="6336-6339" bases="AAGA"/><Component hybridizedRange="6341-6367" bases="Attaggctggagaattaaagtagttgc"/></BindingSite><BindingSite simplified="1" location="6324-6367" boundStrand="0" annealedBases="cttgaaagtcaaAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="6324-6335" bases="cttgaaagtcaa"/><Component bases="GAAG"/><Component hybridizedRange="6340-6367" bases="AAttaggctggagaattaaagtagttgc"/></BindingSite></Primer><Primer recentID="23" name="P692 PriM_mpk_R1" sequence="TGCaagaactacTGCtgcaccTGCgaacatatcaatgttgcttctgtagtc" description="0"><BindingSite location="6585-6632" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6623-6632" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6615-6620" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6585-6611" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite><BindingSite simplified="1" location="6585-6632" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6623-6632" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6615-6620" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6585-6611" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite></Primer><Primer recentID="22" name="P444 pKL75_F" sequence="atatGGTACCagcttaatcagtatcagaactaatacc" description="Contains KpnI site"><BindingSite location="6873-6899" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6873-6899" bases="agcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6873-6899" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6873-6899" bases="agcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="21" name="P695 PriM_mpk_R3" sequence="catcagcTGCtagtgaaactgctttagttgtatcact" description="0"><BindingSite location="6738-6774" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6768-6774" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6738-6764" bases="tagtgaaactgctttagttgtatcact"/></BindingSite><BindingSite simplified="1" location="6738-6774" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6768-6774" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6738-6764" bases="tagtgaaactgctttagttgtatcact"/></BindingSite></Primer><Primer recentID="0" name="P429 FTL0702_VSVG_2R" sequence="atatGAATTCagttgctgaacaagctaaactaaaaag" description="0"><BindingSite location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite><BindingSite simplified="1" location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite></Primer><Primer recentID="1" name="P696 PriM_mpk_F4" sequence="ttcactaGCAgctgatgctaacacacttacatatac" description="0"><BindingSite location="6768-6793" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6768-6793" bases="gctgatgctaacacacttacatatac"/></BindingSite><BindingSite simplified="1" location="6768-6793" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6768-6793" bases="gctgatgctaacacacttacatatac"/></BindingSite></Primer><Primer recentID="3" name="P411 FTL0702sp_2R" sequence="atatGGTACCgcatttgtagctgctttaagtgccc" description="Contains KpnI site"><BindingSite location="7036-7060" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="7036-7060" bases="gcatttgtagctgctttaagtgccc"/></BindingSite><BindingSite simplified="1" location="7036-7060" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="7036-7060" bases="gcatttgtagctgctttaagtgccc"/></BindingSite></Primer><Primer recentID="4" name="P368 FTL0702seqR1" sequence="acttgtagatattaacattaaagtatgac" description="0"><BindingSite location="7105-7133" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="7105-7133" bases="acttgtagatattaacattaaagtatgac"/></BindingSite><BindingSite simplified="1" location="7105-7133" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="7105-7133" bases="acttgtagatattaacattaaagtatgac"/></BindingSite></Primer><Primer recentID="5" name="P430 CkFTL0702V_R" sequence="gtcatactttaatgttaatatctacaagt" description="0"><BindingSite location="7105-7133" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="7105-7133" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite><BindingSite simplified="1" location="7105-7133" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="7105-7133" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite></Primer><Primer recentID="6" name="P704 CkPriM_mtip1R" sequence="aatatcattaaaatacgccttaggacc" description="0"><BindingSite location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite><BindingSite simplified="1" location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite></Primer><Primer recentID="7" name="P367 FTL0702seqF1" sequence="ctcaaggtcttgagaaatacagagc" description="0"><BindingSite location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite></Primer><Primer recentID="9" name="P413 CkFTL0702stp30R" sequence="gctctgtatttctcaagaccttgag" description="0"><BindingSite location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite></Primer><Primer recentID="10" name="P697 Ck_PriM_mpk1F" sequence="cagcatgggattctgctactagtc" description="0"><BindingSite location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite><BindingSite simplified="1" location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite></Primer><Primer recentID="11" name="P437 RT_FTL0702-2F" sequence="agcaagtaagtgatgctgataga" description="0"><BindingSite location="7183-7205" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="7183-7205" bases="agcaagtaagtgatgctgataga"/></BindingSite><BindingSite simplified="1" location="7183-7205" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="7183-7205" bases="agcaagtaagtgatgctgataga"/></BindingSite></Primer><Primer recentID="12" name="P698 Ck_PriM_mpk1R" sequence="gctttaagtgcccagttgtcgcc" description="0"><BindingSite location="7026-7048" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="7026-7048" bases="gctttaagtgcccagttgtcgcc"/></BindingSite><BindingSite simplified="1" location="7026-7048" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="7026-7048" bases="gctttaagtgcccagttgtcgcc"/></BindingSite></Primer><Primer recentID="13" name="P439 RT_FTL0702-3F" sequence="tgctgatgctaacacacttaca" description="0"><BindingSite location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite><BindingSite simplified="1" location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite></Primer><Primer recentID="14" name="P436 RT_FTL0702-1R" sequence="cggcatctttgctattgatgtt" description="0"><BindingSite location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite><BindingSite simplified="1" location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite></Primer><Primer recentID="15" name="P440 RT_FTL0702-3R" sequence="ccgttcttggcgaagctatt" description="0"><BindingSite location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite><BindingSite simplified="1" location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite></Primer><Primer recentID="16" name="P435 RT_FTL0702-1F" sequence="cagcatgggattctgctact" description="0"><BindingSite location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite><BindingSite simplified="1" location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite></Primer><Primer recentID="17" name="KROL19 PriMnoCFR1F" sequence="aatttatgcattcaaaagcatagcagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6107-6133" boundStrand="0" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="6107-6133" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite><BindingSite simplified="1" location="6107-6133" boundStrand="0" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="6107-6133" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite></Primer><Primer recentID="18" name="KROL20 PriMnoC1FR1R" sequence="aattgatagagccgttcttggcgaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6839-6863" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6839-6863" bases="aattgatagagccgttcttggcgaa"/></BindingSite><BindingSite simplified="1" location="6839-6863" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6839-6863" bases="aattgatagagccgttcttggcgaa"/></BindingSite></Primer><Primer recentID="19" name="KROL21 PriMnoC1FR2F" sequence="atatGCtgaagctagcttaatcagtatcagaactaatacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6866-6899" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="58"><Component bases="atatGC"/><Component hybridizedRange="6866-6899" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6866-6899" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="58"><Component bases="atatGC"/><Component hybridizedRange="6866-6899" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="20" name="KROL22 PriMnoC2FR1R" sequence="gtaaaattaGCtgcatttttaccaaatagaactctatca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7199-7237" boundStrand="1" annealedBases="gtaaaattatgcatttttaccaaatagaactctatca" meltingTemperature="51"><Component hybridizedRange="7229-7237" bases="gtaaaatta"/><Component bases="GC"/><Component hybridizedRange="7199-7226" bases="tgcatttttaccaaatagaactctatca"/></BindingSite><BindingSite simplified="1" location="7199-7237" boundStrand="1" annealedBases="gtaaaattatgcatttttaccaaatagaactctatca" meltingTemperature="51"><Component hybridizedRange="7229-7237" bases="gtaaaatta"/><Component bases="GC"/><Component hybridizedRange="7199-7226" bases="tgcatttttaccaaatagaactctatca"/></BindingSite></Primer><Primer recentID="2" name="P702 PriM_mtip1_R2" sequence="atatGGTACCtactttctcttcgaaaacggtgtcg" description="&lt;html&gt;&lt;body&gt;Contains KpnI site&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7328-7358" boundStrand="1" annealedBases="GGTACCtactttctcttcgaaaacggtgtcg" meltingTemperature="63"><Component bases="atat"/><Component hybridizedRange="7328-7358" bases="GGTACCtactttctcttcgaaaacggtgtcg"/></BindingSite><BindingSite simplified="1" location="7328-7358" boundStrand="1" annealedBases="GGTACCtactttctcttcgaaaacggtgtcg" meltingTemperature="63"><Component bases="atat"/><Component hybridizedRange="7328-7358" bases="GGTACCtactttctcttcgaaaacggtgtcg"/></BindingSite></Primer><Primer recentID="8" name="P438 RT_FTL0702-2R" sequence="agttgtccggctatctgtttag" description="0"><BindingSite location="7288-7309" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="7288-7309" bases="agttgtccggctatctgtttag"/></BindingSite><BindingSite simplified="1" location="7288-7309" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="7288-7309" bases="agttgtccggctatctgtttag"/></BindingSite></Primer></Primers>
  D<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="16:40:20">2018.12.17</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NA4VIudNaN]<zP)*DTk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>    M  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #<?xml version="1.0"?><Features recycledIDs="1,2,4,5,6,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25" nextValidID="39"><Feature recentID="38" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-6951" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="37" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6223-6227" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="36" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6657-288" color="#ffff00" type="standard"/><Q name="note"><V text="ColE origin"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="35" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="34" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="32" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="30" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; 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  C<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="16:40:9">2018.12.17</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  p               .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
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ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAjckU6M8cRYMXUB^]`)F|^UF׎;PY8TF]UTQZC$yU&yVFoBlUS8Ql*YUYX/2jmڕ69^bGhf+#Q$X-f]TcxqyyyiK$uM*f8cwftd1I"UVN\;Z8SET9ityeU8~gwy&z~FV^zTӝX֑Bdrgbnu$(cmC8Eju5%!%V6%tӕY%9:tduBԦ|RB|NŬضN*Vo;uw]O6TSa!9{v;x=8X[\£&ξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	          !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6551-6574" color="#50ff50" type="standard"/><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature><Feature recentID="13" name="P701 PriM_mtip1_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6343-6368" color="#50ff50" type="standard"/><Q name="note"><V text="P701 PriM_mtip1_F2"/></Q></Feature><Feature recentID="12" name="P704 CkPriM_mtip1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6496-6522" color="#50ff50" type="standard"/><Q name="note"><V text="P704 CkPriM_mtip1R"/></Q></Feature><Feature recentID="11" name="P705 PriM_mtip2_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6358-6384" color="#50ff50" type="standard"/><Q name="note"><V text="P705 PriM_mtip2_F2"/></Q></Feature><Feature recentID="10" name="P708 PriM_unst1_F1" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6616-6640" color="#50ff50" type="standard"/><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature><Feature recentID="9" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6867-7353" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="8" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6867-7353" color="#00e600" type="standard"/></Feature><Feature recentID="7" name="hypothetical protein" directionality="1" translationMW="17717.02" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="304" maxRunOn="7386" maxFusedRunOn="7386"><Segment range="6867-7353" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="P368 FTL0702seqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7106-7134" color="#8080ff" type="standard"/><Q name="note"><V text="P368 FTL0702seqR1"/></Q></Feature><Feature recentID="5" name="P411 FTL0702sp_2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7037-7061" color="#50ff50" type="standard"/><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="4" name="P437 RT_FTL0702-2F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7184-7206" color="#50ff50" type="standard"/><Q name="note"><V text="P437 RT_FTL0702-2F"/></Q></Feature><Feature recentID="3" name="P438 RT_FTL0702-2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7289-7310" color="#50ff50" type="standard"/><Q name="note"><V text="P438 RT_FTL0702-2R"/></Q></Feature><Feature recentID="2" name="P444 pKL75_F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6874-6900" color="#50ff50" type="standard"/><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="1" name="P698 Ck_PriM_mpk1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7027-7049" color="#50ff50" type="standard"/><Q name="note"><V text="P698 Ck_PriM_mpk1R"/></Q></Feature><Feature recentID="0" name="P702 PriM_mtip1_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7329-7353" color="#50ff50" type="standard"/><Q name="note"><V text="P702 PriM_mtip1_R2"/></Q></Feature></Features>
  2<?xml version="1.0"?><Primers nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="21" name="KROL22 PriMnoC2FR1R" sequence="gtaaaattaGCtgcatttttaccaaatagaactctatca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7199-7237" boundStrand="1" annealedBases="gtaaaattatgcatttttaccaaatagaactctatca" meltingTemperature="51"><Component hybridizedRange="7229-7237" bases="gtaaaatta"/><Component bases="GC"/><Component hybridizedRange="7199-7226" bases="tgcatttttaccaaatagaactctatca"/></BindingSite><BindingSite simplified="1" location="7199-7237" boundStrand="1" annealedBases="gtaaaattatgcatttttaccaaatagaactctatca" meltingTemperature="51"><Component hybridizedRange="7229-7237" bases="gtaaaatta"/><Component bases="GC"/><Component hybridizedRange="7199-7226" bases="tgcatttttaccaaatagaactctatca"/></BindingSite></Primer><Primer recentID="0" name="P821 PriM_mtip1_R3" sequence="atatGGTACCgtaactttggtattagttctgatactg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6881-6907" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6881-6907" bases="gtaactttggtattagttctgatactg"/></BindingSite><BindingSite simplified="1" location="6881-6907" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6881-6907" bases="gtaactttggtattagttctgatactg"/></BindingSite></Primer><Primer recentID="1" name="P444 pKL75_F" sequence="atatGGTACCagcttaatcagtatcagaactaatacc" description="Contains KpnI site"><BindingSite location="6873-6899" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6873-6899" bases="agcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6873-6899" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6873-6899" bases="agcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="2" name="P702 PriM_mtip1_R2" sequence="atatGGTACCtactttctcttcgaaaacggtgtcg" description="&lt;html&gt;&lt;body&gt;Contains KpnI site&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7328-7358" boundStrand="1" annealedBases="GGTACCtactttctcttcgaaaacggtgtcg" meltingTemperature="63"><Component bases="atat"/><Component hybridizedRange="7328-7358" bases="GGTACCtactttctcttcgaaaacggtgtcg"/></BindingSite><BindingSite simplified="1" location="7328-7358" boundStrand="1" annealedBases="GGTACCtactttctcttcgaaaacggtgtcg" meltingTemperature="63"><Component bases="atat"/><Component hybridizedRange="7328-7358" bases="GGTACCtactttctcttcgaaaacggtgtcg"/></BindingSite></Primer><Primer recentID="3" name="P411 FTL0702sp_2R" sequence="atatGGTACCgcatttgtagctgctttaagtgccc" description="Contains KpnI site"><BindingSite location="7036-7060" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="7036-7060" bases="gcatttgtagctgctttaagtgccc"/></BindingSite><BindingSite simplified="1" location="7036-7060" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="7036-7060" bases="gcatttgtagctgctttaagtgccc"/></BindingSite></Primer><Primer recentID="4" name="P368 FTL0702seqR1" sequence="acttgtagatattaacattaaagtatgac" description="0"><BindingSite location="7105-7133" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="7105-7133" bases="acttgtagatattaacattaaagtatgac"/></BindingSite><BindingSite simplified="1" location="7105-7133" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="7105-7133" bases="acttgtagatattaacattaaagtatgac"/></BindingSite></Primer><Primer recentID="5" name="P430 CkFTL0702V_R" sequence="gtcatactttaatgttaatatctacaagt" description="0"><BindingSite location="7105-7133" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="7105-7133" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite><BindingSite simplified="1" location="7105-7133" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="7105-7133" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite></Primer><Primer recentID="6" name="P437 RT_FTL0702-2F" sequence="agcaagtaagtgatgctgataga" description="0"><BindingSite location="7183-7205" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="7183-7205" bases="agcaagtaagtgatgctgataga"/></BindingSite><BindingSite simplified="1" location="7183-7205" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="7183-7205" bases="agcaagtaagtgatgctgataga"/></BindingSite></Primer><Primer recentID="7" name="P698 Ck_PriM_mpk1R" sequence="gctttaagtgcccagttgtcgcc" description="0"><BindingSite location="7026-7048" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="7026-7048" bases="gctttaagtgcccagttgtcgcc"/></BindingSite><BindingSite simplified="1" location="7026-7048" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="7026-7048" bases="gctttaagtgcccagttgtcgcc"/></BindingSite></Primer><Primer recentID="8" name="P438 RT_FTL0702-2R" sequence="agttgtccggctatctgtttag" description="0"><BindingSite location="7288-7309" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="7288-7309" bases="agttgtccggctatctgtttag"/></BindingSite><BindingSite simplified="1" location="7288-7309" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="7288-7309" bases="agttgtccggctatctgtttag"/></BindingSite></Primer><Primer recentID="9" name="KROL21 PriMnoC1FR2F" sequence="atatGCtgaagctagcttaatcagtatcagaactaatacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6863-6899" boundStrand="0" annealedBases="tGCtgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="62"><Component bases="ata"/><Component hybridizedRange="6863-6899" bases="tGCtgaagctagcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6863-6899" boundStrand="0" annealedBases="tGCtgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="62"><Component bases="ata"/><Component hybridizedRange="6863-6899" bases="tGCtgaagctagcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="10" name="P429 FTL0702_VSVG_2R" sequence="atatGAATTCagttgctgaacaagctaaactaaaaag" description="0"><BindingSite location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite><BindingSite simplified="1" location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite></Primer><Primer recentID="11" name="P704 CkPriM_mtip1R" sequence="aatatcattaaaatacgccttaggacc" description="0"><BindingSite location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite><BindingSite simplified="1" location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite></Primer><Primer recentID="12" name="P367 FTL0702seqF1" sequence="ctcaaggtcttgagaaatacagagc" description="0"><BindingSite location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite></Primer><Primer recentID="13" name="P413 CkFTL0702stp30R" sequence="gctctgtatttctcaagaccttgag" description="0"><BindingSite location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite></Primer><Primer recentID="14" name="P697 Ck_PriM_mpk1F" sequence="cagcatgggattctgctactagtc" description="0"><BindingSite location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite><BindingSite simplified="1" location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite></Primer><Primer recentID="15" name="P439 RT_FTL0702-3F" sequence="tgctgatgctaacacacttaca" description="0"><BindingSite location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite><BindingSite simplified="1" location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite></Primer><Primer recentID="16" name="P436 RT_FTL0702-1R" sequence="cggcatctttgctattgatgtt" description="0"><BindingSite location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite><BindingSite simplified="1" location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite></Primer><Primer recentID="17" name="P440 RT_FTL0702-3R" sequence="ccgttcttggcgaagctatt" description="0"><BindingSite location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite><BindingSite simplified="1" location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite></Primer><Primer recentID="18" name="P435 RT_FTL0702-1F" sequence="cagcatgggattctgctact" description="0"><BindingSite location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite><BindingSite simplified="1" location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite></Primer><Primer recentID="19" name="KROL19 PriMnoCFR1F" sequence="atatGGATCCaatttatgcattcaaaagcatagcagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6101-6133" boundStrand="0" annealedBases="GGATCCaatttatgcattcaaaagcatagcagc" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="6101-6133" bases="GGATCCaatttatgcattcaaaagcatagcagc"/></BindingSite><BindingSite simplified="1" location="6101-6133" boundStrand="0" annealedBases="GGATCCaatttatgcattcaaaagcatagcagc" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="6101-6133" bases="GGATCCaatttatgcattcaaaagcatagcagc"/></BindingSite></Primer><Primer recentID="20" name="KROL20 PriMnoC1FR1R" sequence="atatGCaattgatagagccgttcttggcgaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6839-6865" boundStrand="1" annealedBases="GCaattgatagagccgttcttggcgaa" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="6839-6865" bases="GCaattgatagagccgttcttggcgaa"/></BindingSite><BindingSite simplified="1" location="6839-6865" boundStrand="1" annealedBases="GCaattgatagagccgttcttggcgaa" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="6839-6865" bases="GCaattgatagagccgttcttggcgaa"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="16:37:1">2018.12.17</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  M               .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
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n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAjckU6M8cRYMXUB^]`)F|^UF׎;PY8TF]UTQZC$yU&yVFoBlUS8Ql*YUYX/2jmڕ69^bGhf+#Q$X-f]TcxqyyyiK$uM*f8cwftd1I"UVN\;Z9:[I^YJyӑYGVUSWyGzy[Whageiu7M9Սi]d+Ig)@z)fRI>9XVSRR+UcbZM9U]o)ShӮWFO^T-JlwʬQ$'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ      !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  9<?xml version="1.0"?><Features nextValidID="43"><Feature recentID="42" name="P702 PriM_mtip1_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7327-7351" color="#50ff50" type="standard"/><Q name="note"><V text="P702 PriM_mtip1_R2"/></Q></Feature><Feature recentID="41" name="P698 Ck_PriM_mpk1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7025-7047" color="#50ff50" type="standard"/><Q name="note"><V text="P698 Ck_PriM_mpk1R"/></Q></Feature><Feature recentID="40" name="P444 pKL75_F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6872-6898" color="#50ff50" type="standard"/><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="39" name="P438 RT_FTL0702-2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7287-7308" color="#50ff50" type="standard"/><Q name="note"><V text="P438 RT_FTL0702-2R"/></Q></Feature><Feature recentID="10" name="P437 RT_FTL0702-2F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7182-7204" color="#50ff50" type="standard"/><Q name="note"><V text="P437 RT_FTL0702-2F"/></Q></Feature><Feature recentID="9" name="P411 FTL0702sp_2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7035-7059" color="#50ff50" type="standard"/><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="8" name="P368 FTL0702seqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7104-7132" color="#8080ff" type="standard"/><Q name="note"><V text="P368 FTL0702seqR1"/></Q></Feature><Feature recentID="7" name="hypothetical protein" directionality="1" translationMW="17717.02" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="304" maxRunOn="7384" maxFusedRunOn="7384"><Segment range="6865-7351" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="2" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6865-7351" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6865-7351" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="38" name="P708 PriM_unst1_F1" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6616-6640" color="#50ff50" type="standard"/><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature><Feature recentID="37" name="P707 PriM_unst1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6579-6605" color="#50ff50" type="standard"/><Q name="note"><V text="P707 PriM_unst1_R1"/></Q></Feature><Feature recentID="36" name="P705 PriM_mtip2_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6358-6384" color="#50ff50" type="standard"/><Q name="note"><V text="P705 PriM_mtip2_F2"/></Q></Feature><Feature recentID="35" name="P706 PriM_mtip2_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6291-6318" color="#50ff50" type="standard"/><Q name="note"><V text="P706 PriM_mtip2_R1"/></Q></Feature><Feature recentID="34" name="P704 CkPriM_mtip1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6496-6522" color="#50ff50" type="standard"/><Q name="note"><V text="P704 CkPriM_mtip1R"/></Q></Feature><Feature recentID="33" name="P701 PriM_mtip1_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6343-6368" color="#50ff50" type="standard"/><Q name="note"><V text="P701 PriM_mtip1_F2"/></Q></Feature><Feature recentID="32" name="P700 PriM_mtip1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6291-6318" color="#50ff50" type="standard"/><Q name="note"><V text="P700 PriM_mtip1_R1"/></Q></Feature><Feature recentID="31" name="P697 Ck_PriM_mpk1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6551-6574" color="#50ff50" type="standard"/><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature><Feature recentID="30" name="P693 PriM_mpk_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6637-6665" color="#50ff50" type="standard"/><Q name="note"><V text="P693 PriM_mpk_F2"/></Q></Feature><Feature recentID="29" name="P692 PriM_mpk_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6586-6612" color="#50ff50" type="standard"/><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="28" name="P440 RT_FTL0702-3R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6834-6853" color="#50ff50" type="standard"/><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="27" name="P439 RT_FTL0702-3F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6768-6789" color="#50ff50" type="standard"/><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="26" name="P436 RT_FTL0702-1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6649-6670" color="#50ff50" type="standard"/><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="25" name="P435 RT_FTL0702-1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6551-6570" color="#50ff50" type="standard"/><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="24" name="P429 FTL0702_VSVG_2R" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6363-6389" color="#50ff50" type="standard"/><Q name="note"><V text="P429 FTL0702_VSVG_2R"/></Q></Feature><Feature recentID="23" name="P367 FTL0702seqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6266-6290" color="#8080ff" type="standard"/><Q name="note"><V text="P367 FTL0702seqF1"/></Q></Feature><Feature recentID="22" name="hypothetical protein" directionality="1" translationMW="27863.55" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="51" maxRunOn="6867" maxFusedRunOn="6867"><Segment range="6108-6864" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="4" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6864" color="#00e600" type="standard"/></Feature><Feature recentID="3" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6864" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="21" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7378-7394" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="15" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="11" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7786-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="6" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7352-7356" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="5" name="FTL_0882" directionality="2" translateFirstCodonAsMet="1" translationMW="117.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7318" maxFusedRunOn="7318"><Segment range="7352-7356" color="#993366" type="standard" translated="1"/><Q name="product"><V text="apolipoprotein N-acyltransferase"/></Q><Q name="note"><V text="/dnas_title=apolipoprotein N-acyltransferase"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256240" predef="GI"/><V text="3952356" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513602.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8080" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  P<?xml version="1.0"?><Primers recycledIDs="30" nextValidID="32"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="31" name="P821 PriM_mtip1_R3" sequence="atatGGTACCgtaactttggtattagttctgatactg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6879-6905" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6879-6905" bases="gtaactttggtattagttctgatactg"/></BindingSite><BindingSite simplified="1" location="6879-6905" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6879-6905" bases="gtaactttggtattagttctgatactg"/></BindingSite></Primer><Primer recentID="29" name="P444 pKL75_F" sequence="atatGGTACCagcttaatcagtatcagaactaatacc" description="Contains KpnI site"><BindingSite location="6871-6897" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6871-6897" bases="agcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6871-6897" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6871-6897" bases="agcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="28" name="P702 PriM_mtip1_R2" sequence="atatGGTACCtactttctcttcgaaaacggtgtcg" description="Contains KpnI site"><BindingSite location="7326-7356" boundStrand="1" annealedBases="GGTACCtactttctcttcgaaaacggtgtcg" meltingTemperature="63"><Component bases="atat"/><Component hybridizedRange="7326-7356" bases="GGTACCtactttctcttcgaaaacggtgtcg"/></BindingSite><BindingSite simplified="1" location="7326-7356" boundStrand="1" annealedBases="GGTACCtactttctcttcgaaaacggtgtcg" meltingTemperature="63"><Component bases="atat"/><Component hybridizedRange="7326-7356" bases="GGTACCtactttctcttcgaaaacggtgtcg"/></BindingSite></Primer><Primer recentID="27" name="P411 FTL0702sp_2R" sequence="atatGGTACCgcatttgtagctgctttaagtgccc" description="Contains KpnI site"><BindingSite location="7034-7058" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="7034-7058" bases="gcatttgtagctgctttaagtgccc"/></BindingSite><BindingSite simplified="1" location="7034-7058" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="7034-7058" bases="gcatttgtagctgctttaagtgccc"/></BindingSite></Primer><Primer recentID="26" name="P368 FTL0702seqR1" sequence="acttgtagatattaacattaaagtatgac" description="0"><BindingSite location="7103-7131" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="7103-7131" bases="acttgtagatattaacattaaagtatgac"/></BindingSite><BindingSite simplified="1" location="7103-7131" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="7103-7131" bases="acttgtagatattaacattaaagtatgac"/></BindingSite></Primer><Primer recentID="25" name="P430 CkFTL0702V_R" sequence="gtcatactttaatgttaatatctacaagt" description="0"><BindingSite location="7103-7131" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="7103-7131" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite><BindingSite simplified="1" location="7103-7131" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="7103-7131" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite></Primer><Primer recentID="24" name="P437 RT_FTL0702-2F" sequence="agcaagtaagtgatgctgataga" description="0"><BindingSite location="7181-7203" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="7181-7203" bases="agcaagtaagtgatgctgataga"/></BindingSite><BindingSite simplified="1" location="7181-7203" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="7181-7203" bases="agcaagtaagtgatgctgataga"/></BindingSite></Primer><Primer recentID="23" name="P698 Ck_PriM_mpk1R" sequence="gctttaagtgcccagttgtcgcc" description="0"><BindingSite location="7024-7046" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="7024-7046" bases="gctttaagtgcccagttgtcgcc"/></BindingSite><BindingSite simplified="1" location="7024-7046" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="7024-7046" bases="gctttaagtgcccagttgtcgcc"/></BindingSite></Primer><Primer recentID="22" name="P438 RT_FTL0702-2R" sequence="agttgtccggctatctgtttag" description="0"><BindingSite location="7286-7307" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="7286-7307" bases="agttgtccggctatctgtttag"/></BindingSite><BindingSite simplified="1" location="7286-7307" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="7286-7307" bases="agttgtccggctatctgtttag"/></BindingSite></Primer><Primer recentID="21" name="KROL21 PriMnoC1FR2F" sequence="tgaagctagcttaatcagtatcagaactaatacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6864-6897" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="59"><Component hybridizedRange="6864-6897" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6864-6897" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="59"><Component hybridizedRange="6864-6897" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="20" name="P707 PriM_unst1_R1" sequence="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6578-6624" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6615-6624" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6606-6611" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6578-6604" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite><BindingSite simplified="1" location="6578-6624" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6615-6624" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6606-6611" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6578-6604" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite></Primer><Primer recentID="19" name="P708 PriM_unst1_F1" sequence="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6595-6639" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6595-6604" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6606-6611" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6615-6639" bases="ggtgcaagagtagttctttggaatg"/></BindingSite><BindingSite simplified="1" location="6595-6639" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6595-6604" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6606-6611" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6615-6639" bases="ggtgcaagagtagttctttggaatg"/></BindingSite></Primer><Primer recentID="18" name="P705 PriM_mtip2_R1" sequence="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="6290-6317" boundStrand="1" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="6290-6317" boundStrand="1" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="17" name="P706 PriM_mtip2_F2" sequence="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGGTaaagtagttgctgaacaagctaaacta" description="0"><BindingSite location="6345-6383" boundStrand="0" annealedBases="GGCGGTaaagtagttgctgaacaagctaaacta" meltingTemperature="52"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGT"/><Component hybridizedRange="6345-6347" bases="GGC"/><Component hybridizedRange="6349-6350" bases="GG"/><Component hybridizedRange="6356-6383" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite><BindingSite simplified="1" location="6356-6383" boundStrand="0" annealedBases="Taaagtagttgctgaacaagctaaacta" meltingTemperature="52"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGG"/><Component hybridizedRange="6356-6383" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite></Primer><Primer recentID="16" name="P700 PriM_mtip1_R1" sequence="ctaaTTCTTCttgactttcaagCTCTTCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="6290-6340" boundStrand="1" annealedBases="TTCTTttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaa"/><Component hybridizedRange="6336-6340" bases="TTCTT"/><Component bases="C"/><Component hybridizedRange="6324-6335" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="6290-6335" boundStrand="1" annealedBases="ttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaaTTCTTC"/><Component hybridizedRange="6324-6335" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="15" name="P693 PriM_mpk_F2" sequence="GCAggtgcaGCAgtagttcttGCAaatggtcaatataacatcaatagcaaaga" description="0"><BindingSite location="6615-6664" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6615-6620" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6623-6632" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6636-6664" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite><BindingSite simplified="1" location="6615-6664" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6615-6620" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6623-6632" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6636-6664" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite></Primer><Primer recentID="14" name="P701 PriM_mtip1_F2" sequence="atcGAAGAGcttgaaagtcaaGAAGAAttaggctggagaattaaagtagttgc" description="0"><BindingSite location="6324-6367" boundStrand="0" annealedBases="cttgaaagtcaaAAGAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="6324-6335" bases="cttgaaagtcaa"/><Component bases="G"/><Component hybridizedRange="6336-6339" bases="AAGA"/><Component hybridizedRange="6341-6367" bases="Attaggctggagaattaaagtagttgc"/></BindingSite><BindingSite simplified="1" location="6324-6367" boundStrand="0" annealedBases="cttgaaagtcaaAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="6324-6335" bases="cttgaaagtcaa"/><Component bases="GAAG"/><Component hybridizedRange="6340-6367" bases="AAttaggctggagaattaaagtagttgc"/></BindingSite></Primer><Primer recentID="13" name="P692 PriM_mpk_R1" sequence="TGCaagaactacTGCtgcaccTGCgaacatatcaatgttgcttctgtagtc" description="0"><BindingSite location="6585-6632" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6623-6632" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6615-6620" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6585-6611" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite><BindingSite simplified="1" location="6585-6632" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6623-6632" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6615-6620" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6585-6611" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite></Primer><Primer recentID="12" name="P695 PriM_mpk_R3" sequence="catcagcTGCtagtgaaactgctttagttgtatcact" description="0"><BindingSite location="6738-6774" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6768-6774" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6738-6764" bases="tagtgaaactgctttagttgtatcact"/></BindingSite><BindingSite simplified="1" location="6738-6774" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6768-6774" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6738-6764" bases="tagtgaaactgctttagttgtatcact"/></BindingSite></Primer><Primer recentID="11" name="P429 FTL0702_VSVG_2R" sequence="atatGAATTCagttgctgaacaagctaaactaaaaag" description="0"><BindingSite location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite><BindingSite simplified="1" location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite></Primer><Primer recentID="10" name="P696 PriM_mpk_F4" sequence="ttcactaGCAgctgatgctaacacacttacatatac" description="0"><BindingSite location="6768-6793" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6768-6793" bases="gctgatgctaacacacttacatatac"/></BindingSite><BindingSite simplified="1" location="6768-6793" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6768-6793" bases="gctgatgctaacacacttacatatac"/></BindingSite></Primer><Primer recentID="9" name="P704 CkPriM_mtip1R" sequence="aatatcattaaaatacgccttaggacc" description="0"><BindingSite location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite><BindingSite simplified="1" location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite></Primer><Primer recentID="8" name="P367 FTL0702seqF1" sequence="ctcaaggtcttgagaaatacagagc" description="0"><BindingSite location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite></Primer><Primer recentID="7" name="P413 CkFTL0702stp30R" sequence="gctctgtatttctcaagaccttgag" description="0"><BindingSite location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite></Primer><Primer recentID="6" name="P697 Ck_PriM_mpk1F" sequence="cagcatgggattctgctactagtc" description="0"><BindingSite location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite><BindingSite simplified="1" location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite></Primer><Primer recentID="5" name="P439 RT_FTL0702-3F" sequence="tgctgatgctaacacacttaca" description="0"><BindingSite location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite><BindingSite simplified="1" location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite></Primer><Primer recentID="4" name="P436 RT_FTL0702-1R" sequence="cggcatctttgctattgatgtt" description="0"><BindingSite location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite><BindingSite simplified="1" location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite></Primer><Primer recentID="3" name="P440 RT_FTL0702-3R" sequence="ccgttcttggcgaagctatt" description="0"><BindingSite location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite><BindingSite simplified="1" location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite></Primer><Primer recentID="2" name="P435 RT_FTL0702-1F" sequence="cagcatgggattctgctact" description="0"><BindingSite location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite><BindingSite simplified="1" location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite></Primer><Primer recentID="1" name="KROL19 PriMnoC1FR1F" sequence="aatttatgcattcaaaagcatagcagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6107-6133" boundStrand="0" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="6107-6133" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite><BindingSite simplified="1" location="6107-6133" boundStrand="0" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="6107-6133" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite></Primer><Primer recentID="0" name="KROL20 PriMnoC1FR1R" sequence="aattgatagagccgttcttggcgaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6839-6863" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6839-6863" bases="aattgatagagccgttcttggcgaa"/></BindingSite><BindingSite simplified="1" location="6839-6863" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6839-6863" bases="aattgatagagccgttcttggcgaa"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="21:51:22">2018.11.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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ZZEfER)E9:%4Yʑ"ymE)]jj_vih;jhIVnV!mVўZVTdj۪tڑfenMgX%꭪j*[VUva^UjyU]eiIeYMZZQҡ4UZjelyYOWYY&mY*;R,IZCM|NŬضN*Vo;uw]O6TSa!9{v;x=8X[\£&ξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	          !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ;q<?xml version="1.0"?><Features nextValidID="39"><Feature recentID="38" name="P708 PriM_unst1_F1" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6616-6640" color="#50ff50" type="standard"/><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature><Feature recentID="37" name="P707 PriM_unst1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6579-6605" color="#50ff50" type="standard"/><Q name="note"><V text="P707 PriM_unst1_R1"/></Q></Feature><Feature recentID="36" name="P705 PriM_mtip2_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6358-6384" color="#50ff50" type="standard"/><Q name="note"><V text="P705 PriM_mtip2_F2"/></Q></Feature><Feature recentID="35" name="P706 PriM_mtip2_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6291-6318" color="#50ff50" type="standard"/><Q name="note"><V text="P706 PriM_mtip2_R1"/></Q></Feature><Feature recentID="34" name="P704 CkPriM_mtip1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6496-6522" color="#50ff50" type="standard"/><Q name="note"><V text="P704 CkPriM_mtip1R"/></Q></Feature><Feature recentID="33" name="P701 PriM_mtip1_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6343-6368" color="#50ff50" type="standard"/><Q name="note"><V text="P701 PriM_mtip1_F2"/></Q></Feature><Feature recentID="32" name="P700 PriM_mtip1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6291-6318" color="#50ff50" type="standard"/><Q name="note"><V text="P700 PriM_mtip1_R1"/></Q></Feature><Feature recentID="31" name="P697 Ck_PriM_mpk1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6551-6574" color="#50ff50" type="standard"/><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature><Feature recentID="30" name="P693 PriM_mpk_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6637-6665" color="#50ff50" type="standard"/><Q name="note"><V text="P693 PriM_mpk_F2"/></Q></Feature><Feature recentID="29" name="P692 PriM_mpk_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6586-6612" color="#50ff50" type="standard"/><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="28" name="P440 RT_FTL0702-3R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6834-6853" color="#50ff50" type="standard"/><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="27" name="P439 RT_FTL0702-3F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6768-6789" color="#50ff50" type="standard"/><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="26" name="P436 RT_FTL0702-1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6649-6670" color="#50ff50" type="standard"/><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="25" name="P435 RT_FTL0702-1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6551-6570" color="#50ff50" type="standard"/><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="24" name="P429 FTL0702_VSVG_2R" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6363-6389" color="#50ff50" type="standard"/><Q name="note"><V text="P429 FTL0702_VSVG_2R"/></Q></Feature><Feature recentID="23" name="P367 FTL0702seqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6266-6290" color="#8080ff" type="standard"/><Q name="note"><V text="P367 FTL0702seqF1"/></Q></Feature><Feature recentID="22" name="hypothetical protein" directionality="1" translationMW="27863.55" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="51" maxRunOn="6882" maxFusedRunOn="6882"><Segment range="6108-6864" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="4" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6864" color="#00e600" type="standard"/></Feature><Feature recentID="3" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6864" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="21" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7916-7932" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="15" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="11" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8324-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="10" name="FTL_0881" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7183-7458" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952355" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q></Feature><Feature recentID="9" name="FTL_0881" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7159" maxFusedRunOn="7159"><Segment range="7183-7458" color="#993366" type="standard" translated="1"/><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256239" predef="GI"/><V text="3952355" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513601.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q></Feature><Feature recentID="8" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6879-6887" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="7" name="FTL_0880" directionality="1" translateFirstCodonAsMet="1" translationMW="319.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6968" maxFusedRunOn="6968"><Segment range="6879-6887" color="#993366" type="standard" translated="1"/><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="6" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7704-7894" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="5" name="FTL_0882" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7673" maxFusedRunOn="7673"><Segment range="7704-7894" color="#993366" type="standard" translated="1"/><Q name="product"><V text="apolipoprotein N-acyltransferase"/></Q><Q name="note"><V text="/dnas_title=apolipoprotein N-acyltransferase"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256240" predef="GI"/><V text="3952356" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513602.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="2" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6865-6869" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="1" name="FTL_0880" directionality="1" translationMW="75.07" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" maxRunOn="6968" maxFusedRunOn="6968"><Segment range="6865-6869" color="#993366" type="standard" translated="1"/><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8618" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  9$<?xml version="1.0"?><Primers nextValidID="21"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="20" name="P707 PriM_unst1_R1" sequence="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6578-6624" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6615-6624" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6606-6611" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6578-6604" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite><BindingSite simplified="1" location="6578-6624" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6615-6624" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6606-6611" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6578-6604" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite></Primer><Primer recentID="19" name="P708 PriM_unst1_F1" sequence="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6595-6639" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6595-6604" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6606-6611" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6615-6639" bases="ggtgcaagagtagttctttggaatg"/></BindingSite><BindingSite simplified="1" location="6595-6639" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6595-6604" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6606-6611" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6615-6639" bases="ggtgcaagagtagttctttggaatg"/></BindingSite></Primer><Primer recentID="18" name="P705 PriM_mtip2_R1" sequence="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="6290-6317" boundStrand="1" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="6290-6317" boundStrand="1" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="17" name="P706 PriM_mtip2_F2" sequence="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGGTaaagtagttgctgaacaagctaaacta" description="0"><BindingSite location="6345-6383" boundStrand="0" annealedBases="GGCGGTaaagtagttgctgaacaagctaaacta" meltingTemperature="52"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGT"/><Component hybridizedRange="6345-6347" bases="GGC"/><Component hybridizedRange="6349-6350" bases="GG"/><Component hybridizedRange="6356-6383" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite><BindingSite simplified="1" location="6356-6383" boundStrand="0" annealedBases="Taaagtagttgctgaacaagctaaacta" meltingTemperature="52"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGG"/><Component hybridizedRange="6356-6383" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite></Primer><Primer recentID="16" name="P700 PriM_mtip1_R1" sequence="ctaaTTCTTCttgactttcaagCTCTTCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="6290-6340" boundStrand="1" annealedBases="TTCTTttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaa"/><Component hybridizedRange="6336-6340" bases="TTCTT"/><Component bases="C"/><Component hybridizedRange="6324-6335" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="6290-6335" boundStrand="1" annealedBases="ttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaaTTCTTC"/><Component hybridizedRange="6324-6335" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="15" name="P693 PriM_mpk_F2" sequence="GCAggtgcaGCAgtagttcttGCAaatggtcaatataacatcaatagcaaaga" description="0"><BindingSite location="6615-6664" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6615-6620" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6623-6632" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6636-6664" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite><BindingSite simplified="1" location="6615-6664" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6615-6620" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6623-6632" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6636-6664" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite></Primer><Primer recentID="14" name="P701 PriM_mtip1_F2" sequence="atcGAAGAGcttgaaagtcaaGAAGAAttaggctggagaattaaagtagttgc" description="0"><BindingSite location="6324-6367" boundStrand="0" annealedBases="cttgaaagtcaaAAGAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="6324-6335" bases="cttgaaagtcaa"/><Component bases="G"/><Component hybridizedRange="6336-6339" bases="AAGA"/><Component hybridizedRange="6341-6367" bases="Attaggctggagaattaaagtagttgc"/></BindingSite><BindingSite simplified="1" location="6324-6367" boundStrand="0" annealedBases="cttgaaagtcaaAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="6324-6335" bases="cttgaaagtcaa"/><Component bases="GAAG"/><Component hybridizedRange="6340-6367" bases="AAttaggctggagaattaaagtagttgc"/></BindingSite></Primer><Primer recentID="13" name="P692 PriM_mpk_R1" sequence="TGCaagaactacTGCtgcaccTGCgaacatatcaatgttgcttctgtagtc" description="0"><BindingSite location="6585-6632" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6623-6632" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6615-6620" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6585-6611" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite><BindingSite simplified="1" location="6585-6632" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6623-6632" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6615-6620" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6585-6611" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite></Primer><Primer recentID="12" name="P695 PriM_mpk_R3" sequence="catcagcTGCtagtgaaactgctttagttgtatcact" description="0"><BindingSite location="6738-6774" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6768-6774" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6738-6764" bases="tagtgaaactgctttagttgtatcact"/></BindingSite><BindingSite simplified="1" location="6738-6774" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6768-6774" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6738-6764" bases="tagtgaaactgctttagttgtatcact"/></BindingSite></Primer><Primer recentID="11" name="P429 FTL0702_VSVG_2R" sequence="atatGAATTCagttgctgaacaagctaaactaaaaag" description="0"><BindingSite location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite><BindingSite simplified="1" location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite></Primer><Primer recentID="10" name="P696 PriM_mpk_F4" sequence="ttcactaGCAgctgatgctaacacacttacatatac" description="0"><BindingSite location="6768-6793" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6768-6793" bases="gctgatgctaacacacttacatatac"/></BindingSite><BindingSite simplified="1" location="6768-6793" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6768-6793" bases="gctgatgctaacacacttacatatac"/></BindingSite></Primer><Primer recentID="9" name="P704 CkPriM_mtip1R" sequence="aatatcattaaaatacgccttaggacc" description="0"><BindingSite location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite><BindingSite simplified="1" location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite></Primer><Primer recentID="8" name="P367 FTL0702seqF1" sequence="ctcaaggtcttgagaaatacagagc" description="0"><BindingSite location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite></Primer><Primer recentID="7" name="P413 CkFTL0702stp30R" sequence="gctctgtatttctcaagaccttgag" description="0"><BindingSite location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite></Primer><Primer recentID="6" name="P697 Ck_PriM_mpk1F" sequence="cagcatgggattctgctactagtc" description="0"><BindingSite location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite><BindingSite simplified="1" location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite></Primer><Primer recentID="5" name="P439 RT_FTL0702-3F" sequence="tgctgatgctaacacacttaca" description="0"><BindingSite location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite><BindingSite simplified="1" location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite></Primer><Primer recentID="4" name="P436 RT_FTL0702-1R" sequence="cggcatctttgctattgatgtt" description="0"><BindingSite location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite><BindingSite simplified="1" location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite></Primer><Primer recentID="3" name="P440 RT_FTL0702-3R" sequence="ccgttcttggcgaagctatt" description="0"><BindingSite location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite><BindingSite simplified="1" location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite></Primer><Primer recentID="2" name="P435 RT_FTL0702-1F" sequence="cagcatgggattctgctact" description="0"><BindingSite location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite><BindingSite simplified="1" location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite></Primer><Primer recentID="1" name="KROL19 PriMnoC1FR1F" sequence="aatttatgcattcaaaagcatagcagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6107-6133" boundStrand="0" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="6107-6133" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite><BindingSite simplified="1" location="6107-6133" boundStrand="0" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="6107-6133" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite></Primer><Primer recentID="0" name="KROL20 PriMnoC1FR1R" sequence="aattgatagagccgttcttggcgaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6839-6863" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6839-6863" bases="aattgatagagccgttcttggcgaa"/></BindingSite><BindingSite simplified="1" location="6839-6863" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6839-6863" bases="aattgatagagccgttcttggcgaa"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="21:51:17">2018.11.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  6        "        ".	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
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ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAbfV9jefId)jfbeIilߖV*_J^Yx4mM2JRfD}rM%MĩnjnQO6mZ7זUzwYHdfJH$c֪٨jQmhA4gXeJ%ͦ>NWWYIeRFjE6Jg^ڢ%qJaiNa^NvvV_c*Ine.ꑎej훦 &&Zh'j,QUdYa)]deX9>&-cV^UNf^֦[ZjٚDU)zSZOZ%+i6Yif&&j`&UimhjAh꛸*uEmuFIfMmZjVyTy)Y%"ZnڪjyUY%eWn6ꩽ%V:eXkEVTUjdեZje*ZcEZiIZOg]dyaՒaڕ"ě	+e=;Yt'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ          !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  *<?xml version="1.0"?><Features nextValidID="22"><Feature recentID="21" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8186-8202" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="15" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="11" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8594-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="10" name="FTL_0881" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7453-7728" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952355" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q></Feature><Feature recentID="9" name="FTL_0881" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7429" maxFusedRunOn="7429"><Segment range="7453-7728" color="#993366" type="standard" translated="1"/><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256239" predef="GI"/><V text="3952355" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513601.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q></Feature><Feature recentID="8" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7149-7157" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="7" name="FTL_0880" directionality="1" translateFirstCodonAsMet="1" translationMW="319.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7238" maxFusedRunOn="7238"><Segment range="7149-7157" color="#993366" type="standard" translated="1"/><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="6" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7974-8164" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="5" name="FTL_0882" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7943" maxFusedRunOn="7943"><Segment range="7974-8164" color="#993366" type="standard" translated="1"/><Q name="product"><V text="apolipoprotein N-acyltransferase"/></Q><Q name="note"><V text="/dnas_title=apolipoprotein N-acyltransferase"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256240" predef="GI"/><V text="3952356" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513602.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="4" name="FTL_0879" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6702" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952353" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0879"/></Q></Feature><Feature recentID="3" name="FTL_0879" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6113-6702" color="#993366" type="standard" translated="1"/><Q name="product"><V text="beta-lactamase"/></Q><Q name="note"><V text="/dnas_title=beta-lactamase"/></Q><Q name="inference"><V bool="1" text="2004" predef="profile"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256237" predef="GI"/><V text="3952353" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513599.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0879"/></Q><Q name="EC_number"><V text="3.5.2.6"/></Q></Feature><Feature recentID="2" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7131-7139" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="1" name="FTL_0880" directionality="1" translateFirstCodonAsMet="1" translationMW="341.40" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7238" maxFusedRunOn="7238"><Segment range="7131-7139" color="#993366" type="standard" translated="1"/><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8888" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="23:40:14">2018.2.27</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  -        d        dJ9ZDFfy̑cRY<WW_fVT[|FY6VtIn[`J_i:6gbeYK%rY^LBcEED_EFPY&y$ETTUU)TNDe@͙     V  `  c  d7zXZ  ִF !   t/($] 3D].?I*ә_.ZvќP,Vj,ު~mC<ґ8hlFOlY.k(DW`!
 	KJ9rEPܤ$FcprMUq3+Xfg=GjQ!bKZvw|
2F}7"Xۑ6Z֨[I@7$w>r|pI.$3doŅh^mbkK;x NR H"9ߡ-f3}H,W{JM+pc}Q0\ 5՗VIkdL#0%X!
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rN"u7LYh8Tw.=n}d?LM        BC             !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #&<?xml version="1.0"?><Features nextValidID="20"><Feature recentID="0" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7787-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="2" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MNIKKFAKQATVLTFTTALLAGGATQAFA,KETNQKPYKETYGISHITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK*"/></Q></Feature><Feature recentID="3" name="KanR" directionality="1" translationMW="30979.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature recentID="4" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="6" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7379-7395" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="FTL_1046" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6250" color="#00e600" type="standard"/></Feature><Feature recentID="12" name="D-alanyl-D-alanine carboxypeptidase" directionality="2" translationMW="5280.24" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="5858" maxFusedRunOn="5858"><Segment range="6108-6250" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="LKRKTKFFTAFSILLISIATTQASTRSEVQYLVKKYNLSDAKIAIAT"/></Q></Feature><Feature recentID="13" name="rpsU2" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6506-6700" color="#00e600" type="standard"/></Feature><Feature recentID="14" name="30S ribosomal protein S21" directionality="1" translationMW="7840.31" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6739" maxFusedRunOn="6739"><Segment range="6506-6700" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MPSVRIKEREPFDVALRRFKRSCEKAGIVSELRRREYFEKPTWARKRKKTAAVKRAHKSNIIVKR"/></Q></Feature><Feature recentID="15" name="FTL_1048" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6800-7246" color="#00e600" type="standard"/></Feature><Feature recentID="16" name="hypothetical protein" directionality="1" translationMW="16766.68" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6800-7246" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSQIFDQLTLDMKESMKNKDKNRLTTIRMAISAIKQKQIDEKIQITDEVVIAVITKMIKQRQDSFDQYIKANRAELAEGEKREIEILTQYMPKQLNDEEVVAIVQKAIESVGATSMKEMGKIMASVKKELAGRTDMSKVSAIVKSNLA*"/></Q></Feature><Feature recentID="17" name="FTL_1049" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7267-7353" color="#00e600" type="standard"/></Feature><Feature recentID="18" name="DNA primase" directionality="1" translationMW="3210.79" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7374" maxFusedRunOn="7374"><Segment range="7267-7353" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MAKKVSNSFIKELVATADIVDVVSRYVNL"/></Q></Feature><Feature recentID="19" name="bS21-2-HA" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6710-6739" color="#993366" type="standard"/></Feature></Features>
  j<?xml version="1.0"?><Primers nextValidID="8"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2345-2354" boundStrand="0" annealedBases="TGAGCGGATA" meltingTemperature="27"><Component bases="TGTGGAATTG"/><Component hybridizedRange="2345-2354" bases="TGAGCGGATA"/></BindingSite><BindingSite location="7406-7425" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="7406-7425" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite location="7656-7666" boundStrand="1" annealedBases="GTGAGCGATA" meltingTemperature="21"><Component bases="TGTGGAATT"/><Component hybridizedRange="7661-7666" bases="GTGAGC"/><Component bases="G"/><Component hybridizedRange="7656-7659" bases="GATA"/></BindingSite><BindingSite simplified="1" location="2345-2354" boundStrand="0" annealedBases="TGAGCGGATA" meltingTemperature="27"><Component bases="TGTGGAATTG"/><Component hybridizedRange="2345-2354" bases="TGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7406-7425" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="7406-7425" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7656-7666" boundStrand="1" annealedBases="GTGAGCGATA" meltingTemperature="21"><Component bases="TGTGGAATT"/><Component hybridizedRange="7661-7666" bases="GTGAGC"/><Component bases="G"/><Component hybridizedRange="7656-7659" bases="GATA"/></BindingSite></Primer><Primer recentID="1" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="2" name="KROL685 rpsU2_HA_FR1_F" sequence="atatGGATCCtgagttgctatagcaattttagcatca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2024-04-02T19:07:50Z"><BindingSite location="6101-6133" boundStrand="0" annealedBases="GGATCCtgagttgctatagcaattttagcatca" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="6101-6133" bases="GGATCCtgagttgctatagcaattttagcatca"/></BindingSite><BindingSite simplified="1" location="6101-6133" boundStrand="0" annealedBases="GGATCCtgagttgctatagcaattttagcatca" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="6101-6133" bases="GGATCCtgagttgctatagcaattttagcatca"/></BindingSite></Primer><Primer recentID="3" name="KROL686 rpsU2_HA_FR1_R" sequence="atatTGCGGCCGCccttttaactattatattgctcttatgag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2024-04-02T19:07:50Z"><BindingSite location="6671-6708" boundStrand="1" annealedBases="TGCGGCCGCccttttaactattatattgctcttatgag" meltingTemperature="66"><Component bases="atat"/><Component hybridizedRange="6671-6708" bases="TGCGGCCGCccttttaactattatattgctcttatgag"/></BindingSite><BindingSite simplified="1" location="6671-6708" boundStrand="1" annealedBases="TGCGGCCGCccttttaactattatattgctcttatgag" meltingTemperature="66"><Component bases="atat"/><Component hybridizedRange="6671-6708" bases="TGCGGCCGCccttttaactattatattgctcttatgag"/></BindingSite></Primer><Primer recentID="4" name="KROL687 rpsU2_HA_FR2_F" sequence="atatGCGGCCGCATACCCATACGATGTTCCAGATTATGCTTAAttaaatttaattcatcgttgagagtttaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2024-04-02T19:08:31Z"><BindingSite location="6700-6768" boundStrand="0" annealedBases="GCGGCCGCATACCCATACGATGTTCCAGATTATGCTTAAttaaatttaattcatcgttgagagtttaac" meltingTemperature="69"><Component bases="atat"/><Component hybridizedRange="6700-6768" bases="GCGGCCGCATACCCATACGATGTTCCAGATTATGCTTAAttaaatttaattcatcgttgagagtttaac"/></BindingSite><BindingSite location="6762-6772" boundStrand="1" annealedBases="gagagttaac" meltingTemperature="16"><Component bases="atatGCGGCCGCATACCCATACGATGTTCCAGATTATGCTTAAttaaatttaattcatcgtt"/><Component hybridizedRange="6766-6772" bases="gagagtt"/><Component bases="t"/><Component hybridizedRange="6762-6764" bases="aac"/></BindingSite><BindingSite simplified="1" location="6700-6768" boundStrand="0" annealedBases="GCGGCCGCATACCCATACGATGTTCCAGATTATGCTTAAttaaatttaattcatcgttgagagtttaac" meltingTemperature="69"><Component bases="atat"/><Component hybridizedRange="6700-6768" bases="GCGGCCGCATACCCATACGATGTTCCAGATTATGCTTAAttaaatttaattcatcgttgagagtttaac"/></BindingSite><BindingSite simplified="1" location="6762-6772" boundStrand="1" annealedBases="gagagttaac" meltingTemperature="16"><Component bases="atatGCGGCCGCATACCCATACGATGTTCCAGATTATGCTTAAttaaatttaattcatcgtt"/><Component hybridizedRange="6766-6772" bases="gagagtt"/><Component bases="t"/><Component hybridizedRange="6762-6764" bases="aac"/></BindingSite></Primer><Primer recentID="5" name="KROL688 rpsU2_HA_FR2_R" sequence="atatGGTACCaagttaacatatcttgaaacaacatcaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2024-04-02T19:08:31Z"><BindingSite location="7323-7357" boundStrand="1" annealedBases="GGTACCaagttaacatatcttgaaacaacatcaac" meltingTemperature="59"><Component bases="atat"/><Component hybridizedRange="7323-7357" bases="GGTACCaagttaacatatcttgaaacaacatcaac"/></BindingSite><BindingSite simplified="1" location="7323-7357" boundStrand="1" annealedBases="GGTACCaagttaacatatcttgaaacaacatcaac" meltingTemperature="59"><Component bases="atat"/><Component hybridizedRange="7323-7357" bases="GGTACCaagttaacatatcttgaaacaacatcaac"/></BindingSite></Primer><Primer recentID="6" name="KROL148 PC_ primer_∆rpsU_ R" sequence="cgccatactgattaaagcttcaacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2024-07-15T14:55:49Z"><BindingSite location="7247-7271" boundStrand="1" annealedBases="cgccatactgattaaagcttcaacc" meltingTemperature="59"><Component hybridizedRange="7247-7271" bases="cgccatactgattaaagcttcaacc"/></BindingSite><BindingSite simplified="1" location="7247-7271" boundStrand="1" annealedBases="cgccatactgattaaagcttcaacc" meltingTemperature="59"><Component hybridizedRange="7247-7271" bases="cgccatactgattaaagcttcaacc"/></BindingSite></Primer><Primer recentID="7" name="KROL343 qPCR-rpsU2-F " sequence="ttggtagagggacattagtcct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2024-07-15T14:57:03Z"><BindingSite location="6437-6458" boundStrand="0" annealedBases="ttggtagagggacattagtcct" meltingTemperature="57"><Component hybridizedRange="6437-6458" bases="ttggtagagggacattagtcct"/></BindingSite><BindingSite simplified="1" location="6437-6458" boundStrand="0" annealedBases="ttggtagagggacattagtcct" meltingTemperature="57"><Component hybridizedRange="6437-6458" bases="ttggtagagggacattagtcct"/></BindingSite></Primer></Primers>
  b<Notes>
<UUID>fe28471d-7fb3-4231-a4d4-f0973c6b1621</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="19:9:29">2024.4.2</Created>
<LastModified UTC="14:57:3">2024.7.15</LastModified>
<CreatedBy>Trial User</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         6+ Cutters                                                                                                   d     CHO                E             Y? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
