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 - C 	
	  !2  ,  /01&'(`
  %j  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                    	                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
                                                                                                                                                                                                                                                                                    	                                                                                                                                                          	                              
                  '         	   0   0                                                                                                                                                                                        	                                                                                                                                                                                                                                                                                                                                                                                        
                                                                                                                	                                                      	          
      '   	 0 0        D  EB  EP  En  G  Ih  L`I!  *\>~	0 5e	25	 3n  \N\\\Y_
	_) & zm
Y= 5?	 5-
B	`i
n 3y	T u  x				 	? 	F_ 
 R* ?  5&(-
7S Mh	. 1*K"pT u  YDMT u  `>V `m4e	 x		
. "v
	 G*P ? do			*	+
 {L P Y^P Yb	 6YRi
E YK *]	7,	K  7MSh	
xf	?	 .? d	@ p	Kli



\ 1Kw
J \9z}K	* ' ^pv	 O;	,
;
J \z}K	9 	 J \_b9z}K	a	F
\  1^Kpwv	;X
*	gv8	70	M!	6R	~     &8	b6R	'f{u	n 3Iz	)Y^V\  (Hcj |0SQ	 ;
)y    !%:Hb5		nmG	

w
V\:b5	_ba	F
f{u'	tKz `
#	 +V	
 *Zc	,7_,		G

,_5a	
  6@	L _<W>	
 
 ; @  zepx<		
* a(jU v   ^ZpEw		
 * L a(_j(0w7 vxU$mQt		^		r


c  C	I ?F			U \ v   1^7KZp3wEY0w		 
U ] v    2A^LZhpgxEw/U9	w	)
8

F j	 *( :NV+|Q h"4	L c    _W0C	  *xvG	                 9 OU_QP
cN)|,_	w%M|
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}J֡ ԹzFgf$iPR	jтY*^8ѠcXei^hׂ%QT#'ڎW9viUm=bWť]UiZDUNb_`C8?8J8Yy6_ 
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M4iUPnQp;H{toamU~FiU*Rfa%zJ}c71+odȑgۃ#a1FY_OOש斊<9RJJY*3\hCl-*	;¿\mCآ5U0/lIJ4m킘46Z-o%긇{_RH2pgdzHW+{+7nL]V<TwbiM
ha0gfbjEVWV@+ݫU   E   J  g        47zXZ  ִF !   t/N
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6)l=Ţį!Z*Z85_̽ekX[gܯIɷG^^n`O9ME{	6yrjqBH,)3`̥dC    # (  %g    YZ     j             9=UGC@)@o֍8e|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_90l   D   I   R   T   ~    7zXZ  ִF !   t/t] 3D].?I*ә_.ZvќP,Vj,ު~mC<ґ8hlFOlY.k(DW`!
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%[uctIU#(W	^W?_0x{/]w`[MIB LIolY.W5R]3"$p@^$!?& )ʲa:lê>$dp2d]$Sfv{y7-BkV  /8 /  qWg    YZ     i             9=UGC@)@o֍8e|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    D   I   R   T   ~    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="81-87" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="15-62" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="108-126" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="125-125" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-612" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="163-612" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="163-612" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-253" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="314-334" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-127" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="613-662" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="233-252" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="233-252" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="233-252" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="233-252" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="313-333" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="313-333" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="313-333" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="313-333" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:47:54">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     h             Uʑ 9Z4b	gdYUIUVZSmlЩ18{);NFxӞxMN8nS׍TY^eYT׉gYeF`E
#gS֞RcQqz^%Ҕ%$RNXQIl놵fZJUU;   ;   @   I   K   u    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-78" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="99-117" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="116-116" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="118-603" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="154-603" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="154-603" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="225-244" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="305-325" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="118-118" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="604-653" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="224-243" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="224-243" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="224-243" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="224-243" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="304-324" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="304-324" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="304-324" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="304-324" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:47:10">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     g             Uʑ 9Z4ᙋ'륜{yf!fVA&aUYiNꛩB@ cU:ӕ;GNy58㉹O^5RRezAeS^&gMePR%uC+aNZyIEUxJSP^	H9bJuE%VjZ՚j*UU`C$   ;   @   I   J   t    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-77" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-116" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-115" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="117-602" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="153-602" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="153-602" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="224-243" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="304-324" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="117-117" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="603-652" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="223-242" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="223-242" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="223-242" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="223-242" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="303-323" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="303-323" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="303-323" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="303-323" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:33">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     f             Uʑ 9Z4ᘱ|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   r    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-75" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="96-114" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-113" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-600" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="151-600" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="151-600" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="222-241" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="302-322" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-115" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-650" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="221-240" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="221-240" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="221-240" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="221-240" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="301-321" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="301-321" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="301-321" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="301-321" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:28">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     e             Uʑ 9Z4|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   n    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  X<?xml version="1.0"?><Features recycledIDs="10,11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="92-110" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="109-109" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="111-596" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="147-596" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="147-596" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="218-237" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="298-318" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="111-111" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="597-646" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="217-236" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="217-236" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="217-236" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="217-236" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="297-317" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="297-317" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="297-317" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="297-317" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:14">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  13   d  Q          Uʑ 9Z4JHVYaUjUgꩶTZjzbފj*E($4Ji{Yjߩ%Qj֔e-ehijbD%&aaeھZ&QOdI}VƄ냦ZQje:][)}TzzAJFe؋G&MjfrީiJ(IVY:fS>U*.JQ%IEDnվ$Z&m:th`a&[H)RݴvU*i4ednjqKinud=mYVM;RUVU^$VV: ɪiEUTf۔ꚕ9F
/T -h{Ev	YkTY*=`UnUNmYe
dKmT<떪aR%)f(gUJjC%4flCNZ*yJIGmj@NZWJZZ1.JYeknb[sYj>Rnj(IibZYfNijM^EBڙzJ^Zb虩[Jf9v^ɤ|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   G  
/  
j    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="21"><Feature recentID="20" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="122-2608" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="19" name="FTL_0126" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="122-2584" color="#00e600" type="standard"/></Feature><Feature recentID="18" name="hypothetical protein" directionality="2" translationMW="95341.69" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="122-2584" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2608-2608" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="16" name="pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="122-2584" color="#a6acb3" type="standard"/></Feature><Feature recentID="15" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2609-2627" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2610-2610" color="#a6acb3" type="standard"/></Feature><Feature recentID="13" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-121" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="12" name="FTL_0125" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="72-117" color="#00e600" type="standard"/></Feature><Feature recentID="11" name="hypothetical protein" directionality="2" translationMW="1785.21" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="28" maxFusedRunOn="28"><Segment range="72-117" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="pdpB" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-117" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2666" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2665-2665" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2667-3152" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2703-3152" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2703-3152" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2774-2793" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="2854-2874" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2667-2667" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3153-3202" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P823 DFPI_FR1R2" sequence="atatGCGGCCGCtgctatcattgtctacttaattagaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2574-2602" boundStrand="0" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="2574-2602" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite><BindingSite simplified="1" location="2574-2602" boundStrand="0" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="2574-2602" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite></Primer><Primer recentID="1" name="KROL311  pdpA_qPCR_R1" sequence="cacttgatctttgtatcgcactatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2133-2157" boundStrand="0" annealedBases="cacttgatctttgtatcgcactatc" meltingTemperature="57"><Component hybridizedRange="2133-2157" bases="cacttgatctttgtatcgcactatc"/></BindingSite><BindingSite simplified="1" location="2133-2157" boundStrand="0" annealedBases="cacttgatctttgtatcgcactatc" meltingTemperature="57"><Component hybridizedRange="2133-2157" bases="cacttgatctttgtatcgcactatc"/></BindingSite></Primer><Primer recentID="2" name="KROL310  pdpA_qPCR_F1" sequence="cttgagctccctcatgattaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2190-2211" boundStrand="1" annealedBases="cttgagctccctcatgattaca" meltingTemperature="57"><Component hybridizedRange="2190-2211" bases="cttgagctccctcatgattaca"/></BindingSite><BindingSite simplified="1" location="2190-2211" boundStrand="1" annealedBases="cttgagctccctcatgattaca" meltingTemperature="57"><Component hybridizedRange="2190-2211" bases="cttgagctccctcatgattaca"/></BindingSite></Primer><Primer recentID="3" name="KROL312  pdpA_qPCR_F2" sequence="aatcatgagggagctcaagaatta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2193-2216" boundStrand="0" annealedBases="aatcatgagggagctcaagaatta" meltingTemperature="55"><Component hybridizedRange="2193-2216" bases="aatcatgagggagctcaagaatta"/></BindingSite><BindingSite simplified="1" location="2193-2216" boundStrand="0" annealedBases="aatcatgagggagctcaagaatta" meltingTemperature="55"><Component hybridizedRange="2193-2216" bases="aatcatgagggagctcaagaatta"/></BindingSite></Primer><Primer recentID="4" name="KROL313  pdpA_qPCR_R2" sequence="cgacaaactataaagactct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2269-2288" boundStrand="1" annealedBases="cgacaaactataaagactct" meltingTemperature="50"><Component hybridizedRange="2269-2288" bases="cgacaaactataaagactct"/></BindingSite><BindingSite simplified="1" location="2269-2288" boundStrand="1" annealedBases="cgacaaactataaagactct" meltingTemperature="50"><Component hybridizedRange="2269-2288" bases="cgacaaactataaagactct"/></BindingSite></Primer><Primer recentID="5" name="KROL314  pdpA_qPCR_F3" sequence="acccaatatatcgtgaaaggct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2067-2088" boundStrand="0" annealedBases="acccaatatatcgtgaaaggct" meltingTemperature="56"><Component hybridizedRange="2067-2088" bases="acccaatatatcgtgaaaggct"/></BindingSite><BindingSite simplified="1" location="2067-2088" boundStrand="0" annealedBases="acccaatatatcgtgaaaggct" meltingTemperature="56"><Component hybridizedRange="2067-2088" bases="acccaatatatcgtgaaaggct"/></BindingSite></Primer><Primer recentID="6" name="KROL315  pdpA_qPCR_R3" sequence="gatagtgcgatacaaagatcaagtg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2133-2157" boundStrand="1" annealedBases="gatagtgcgatacaaagatcaagtg" meltingTemperature="56"><Component hybridizedRange="2133-2157" bases="gatagtgcgatacaaagatcaagtg"/></BindingSite><BindingSite simplified="1" location="2133-2157" boundStrand="1" annealedBases="gatagtgcgatacaaagatcaagtg" meltingTemperature="56"><Component hybridizedRange="2133-2157" bases="gatagtgcgatacaaagatcaagtg"/></BindingSite></Primer><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="2773-2792" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="2773-2792" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="2773-2792" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="2773-2792" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="2853-2873" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="2853-2873" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="2853-2873" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="2853-2873" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:44:19">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     l  p          9 OU_QP
cN)|,_	w%M|
G+,a)'\!_1|RKb	w}|4z _W^Fn}r,j|GEs	tWBDQ]E/B0s\R!W69v^ɤ|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_90 Î]SPS|5e\ Ao:x{8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%ˡ/_QzlM=A1	#9EI&;TJ	;5u}L FUE sqWFPnoVXR[[&fWt:[HC~nJ>,yp:}?8jm5P<LH/zϽ.ihS%$5(t6"철:Ӽ+1)o#US
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%[uctIU#(W	^W?_0x{/]w`[MIB LIolY.W5R]3"$p@^$!?& )ʲa:lê>$dp2d]$Sfv{y7-BkV  /8 /  qWg    YZ     s             9=UGC@)@o֍8e|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    D   I   R   T   ~    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="81-87" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="15-62" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="108-126" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="125-125" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-612" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="163-612" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="163-612" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-253" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="314-334" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-127" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="613-662" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="233-252" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="233-252" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="233-252" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="233-252" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="313-333" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="313-333" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="313-333" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="313-333" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:47:54">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     r             Uʑ 9Z4b	gdYUIUVZSmlЩ18{);NFxӞxMN8nS׍TY^eYT׉gYeF`E
#gS֞RcQqz^%Ҕ%$RNXQIl놵fZJUU;   ;   @   I   K   u    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-78" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="99-117" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="116-116" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="118-603" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="154-603" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="154-603" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="225-244" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="305-325" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="118-118" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="604-653" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="224-243" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="224-243" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="224-243" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="224-243" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="304-324" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="304-324" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="304-324" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="304-324" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:47:10">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     q             Uʑ 9Z4ᙋ'륜{yf!fVA&aUYiNꛩB@ cU:ӕ;GNy58㉹O^5RRezAeS^&gMePR%uC+aNZyIEUxJSP^	H9bJuE%VjZ՚j*UU`C$   ;   @   I   J   t    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-77" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-116" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-115" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="117-602" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="153-602" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="153-602" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="224-243" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="304-324" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="117-117" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="603-652" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="223-242" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="223-242" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="223-242" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="223-242" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="303-323" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="303-323" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="303-323" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="303-323" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:33">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     p             Uʑ 9Z4ᘱ|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   r    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-75" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="96-114" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-113" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-600" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="151-600" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="151-600" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="222-241" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="302-322" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-115" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-650" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="221-240" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="221-240" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="221-240" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="221-240" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="301-321" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="301-321" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="301-321" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="301-321" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:28">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     o             Uʑ 9Z4|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   n    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  X<?xml version="1.0"?><Features recycledIDs="10,11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="92-110" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="109-109" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="111-596" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="147-596" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="147-596" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="218-237" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="298-318" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="111-111" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="597-646" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="217-236" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="217-236" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="217-236" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="217-236" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="297-317" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="297-317" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="297-317" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="297-317" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:14">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  13   n  Q          Uʑ 9Z4JHVYaUjUgꩶTZjzbފj*E($4Ji{Yjߩ%Qj֔e-ehijbD%&aaeھZ&QOdI}VƄ냦ZQje:][)}TzzAJFe؋G&MjfrީiJ(IVY:fS>U*.JQ%IEDnվ$Z&m:th`a&[H)RݴvU*i4ednjqKinud=mYVM;RUVU^$VV: ɪiEUTf۔ꚕ9F
/T -h{Ev	YkTY*=`UnUNmYe
dKmT<떪aR%)f(gUJjC%4flCNZ*yJIGmj@NZWJZZ1.JYeknb[sYj>Rnj(IibZYfNijM^EBڙzJ^Zb虩[Jf9v^ɤ|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   G  
/  
j    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="21"><Feature recentID="20" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="122-2608" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="19" name="FTL_0126" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="122-2584" color="#00e600" type="standard"/></Feature><Feature recentID="18" name="hypothetical protein" directionality="2" translationMW="95341.69" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="122-2584" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2608-2608" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="16" name="pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="122-2584" color="#a6acb3" type="standard"/></Feature><Feature recentID="15" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2609-2627" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2610-2610" color="#a6acb3" type="standard"/></Feature><Feature recentID="13" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-121" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="12" name="FTL_0125" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="72-117" color="#00e600" type="standard"/></Feature><Feature recentID="11" name="hypothetical protein" directionality="2" translationMW="1785.21" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="28" maxFusedRunOn="28"><Segment range="72-117" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="pdpB" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-117" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2666" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2665-2665" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2667-3152" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2703-3152" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2703-3152" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2774-2793" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="2854-2874" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2667-2667" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3153-3202" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P823 DFPI_FR1R2" sequence="atatGCGGCCGCtgctatcattgtctacttaattagaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2574-2602" boundStrand="0" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="2574-2602" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite><BindingSite simplified="1" location="2574-2602" boundStrand="0" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="2574-2602" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite></Primer><Primer recentID="1" name="KROL311  pdpA_qPCR_R1" sequence="cacttgatctttgtatcgcactatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2133-2157" boundStrand="0" annealedBases="cacttgatctttgtatcgcactatc" meltingTemperature="57"><Component hybridizedRange="2133-2157" bases="cacttgatctttgtatcgcactatc"/></BindingSite><BindingSite simplified="1" location="2133-2157" boundStrand="0" annealedBases="cacttgatctttgtatcgcactatc" meltingTemperature="57"><Component hybridizedRange="2133-2157" bases="cacttgatctttgtatcgcactatc"/></BindingSite></Primer><Primer recentID="2" name="KROL310  pdpA_qPCR_F1" sequence="cttgagctccctcatgattaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2190-2211" boundStrand="1" annealedBases="cttgagctccctcatgattaca" meltingTemperature="57"><Component hybridizedRange="2190-2211" bases="cttgagctccctcatgattaca"/></BindingSite><BindingSite simplified="1" location="2190-2211" boundStrand="1" annealedBases="cttgagctccctcatgattaca" meltingTemperature="57"><Component hybridizedRange="2190-2211" bases="cttgagctccctcatgattaca"/></BindingSite></Primer><Primer recentID="3" name="KROL312  pdpA_qPCR_F2" sequence="aatcatgagggagctcaagaatta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2193-2216" boundStrand="0" annealedBases="aatcatgagggagctcaagaatta" meltingTemperature="55"><Component hybridizedRange="2193-2216" bases="aatcatgagggagctcaagaatta"/></BindingSite><BindingSite simplified="1" location="2193-2216" boundStrand="0" annealedBases="aatcatgagggagctcaagaatta" meltingTemperature="55"><Component hybridizedRange="2193-2216" bases="aatcatgagggagctcaagaatta"/></BindingSite></Primer><Primer recentID="4" name="KROL313  pdpA_qPCR_R2" sequence="cgacaaactataaagactct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2269-2288" boundStrand="1" annealedBases="cgacaaactataaagactct" meltingTemperature="50"><Component hybridizedRange="2269-2288" bases="cgacaaactataaagactct"/></BindingSite><BindingSite simplified="1" location="2269-2288" boundStrand="1" annealedBases="cgacaaactataaagactct" meltingTemperature="50"><Component hybridizedRange="2269-2288" bases="cgacaaactataaagactct"/></BindingSite></Primer><Primer recentID="5" name="KROL314  pdpA_qPCR_F3" sequence="acccaatatatcgtgaaaggct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2067-2088" boundStrand="0" annealedBases="acccaatatatcgtgaaaggct" meltingTemperature="56"><Component hybridizedRange="2067-2088" bases="acccaatatatcgtgaaaggct"/></BindingSite><BindingSite simplified="1" location="2067-2088" boundStrand="0" annealedBases="acccaatatatcgtgaaaggct" meltingTemperature="56"><Component hybridizedRange="2067-2088" bases="acccaatatatcgtgaaaggct"/></BindingSite></Primer><Primer recentID="6" name="KROL315  pdpA_qPCR_R3" sequence="gatagtgcgatacaaagatcaagtg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2133-2157" boundStrand="1" annealedBases="gatagtgcgatacaaagatcaagtg" meltingTemperature="56"><Component hybridizedRange="2133-2157" bases="gatagtgcgatacaaagatcaagtg"/></BindingSite><BindingSite simplified="1" location="2133-2157" boundStrand="1" annealedBases="gatagtgcgatacaaagatcaagtg" meltingTemperature="56"><Component hybridizedRange="2133-2157" bases="gatagtgcgatacaaagatcaagtg"/></BindingSite></Primer><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="2773-2792" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="2773-2792" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="2773-2792" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="2773-2792" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="2853-2873" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="2853-2873" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="2853-2873" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="2853-2873" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:44:19">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  f0󘺟waiٚ(j`q	ЖaA)ZRuPJ'Y`kYF}SaQH)+l\cZR떙@٥BKh'FudxF"Uaymz~g^
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B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    D   I   R   T   ~    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="81-87" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="15-62" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="108-126" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="125-125" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-612" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="163-612" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="163-612" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-253" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="314-334" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-127" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="613-662" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="233-252" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="233-252" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="233-252" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="233-252" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="313-333" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="313-333" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="313-333" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="313-333" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:47:54">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     %             Uʑ 9Z4b	gdYUIUVZSmlЩ18{);NFxӞxMN8nS׍TY^eYT׉gYeF`E
#gS֞RcQqz^%Ҕ%$RNXQIl놵fZJUU;   ;   @   I   K   u    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-78" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="99-117" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="116-116" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="118-603" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="154-603" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="154-603" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="225-244" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="305-325" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="118-118" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="604-653" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="224-243" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="224-243" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="224-243" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="224-243" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="304-324" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="304-324" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="304-324" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="304-324" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:47:10">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     $             Uʑ 9Z4ᙋ'륜{yf!fVA&aUYiNꛩB@ cU:ӕ;GNy58㉹O^5RRezAeS^&gMePR%uC+aNZyIEUxJSP^	H9bJuE%VjZ՚j*UU`C$   ;   @   I   J   t    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-77" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-116" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-115" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="117-602" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="153-602" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="153-602" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="224-243" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="304-324" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="117-117" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="603-652" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="223-242" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="223-242" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="223-242" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="223-242" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="303-323" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="303-323" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="303-323" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="303-323" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:33">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     #             Uʑ 9Z4ᘱ|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   r    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-75" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="96-114" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-113" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-600" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="151-600" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="151-600" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="222-241" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="302-322" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-115" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-650" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="221-240" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="221-240" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="221-240" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="221-240" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="301-321" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="301-321" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="301-321" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="301-321" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:28">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     "             Uʑ 9Z4|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   n    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  X<?xml version="1.0"?><Features recycledIDs="10,11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="92-110" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="109-109" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="111-596" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="147-596" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="147-596" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="218-237" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="298-318" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="111-111" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="597-646" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="217-236" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="217-236" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="217-236" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="217-236" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="297-317" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="297-317" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="297-317" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="297-317" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:14">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  13   !  Q          Uʑ 9Z4JHVYaUjUgꩶTZjzbފj*E($4Ji{Yjߩ%Qj֔e-ehijbD%&aaeھZ&QOdI}VƄ냦ZQje:][)}TzzAJFe؋G&MjfrީiJ(IVY:fS>U*.JQ%IEDnվ$Z&m:th`a&[H)RݴvU*i4ednjqKinud=mYVM;RUVU^$VV: ɪiEUTf۔ꚕ9F
/T -h{Ev	YkTY*=`UnUNmYe
dKmT<떪aR%)f(gUJjC%4flCNZ*yJIGmj@NZWJZZ1.JYeknb[sYj>Rnj(IibZYfNijM^EBڙzJ^Zb虩[Jf9v^ɤ|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   G  
/  
j    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="21"><Feature recentID="20" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="122-2608" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="19" name="FTL_0126" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="122-2584" color="#00e600" type="standard"/></Feature><Feature recentID="18" name="hypothetical protein" directionality="2" translationMW="95341.69" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="122-2584" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2608-2608" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="16" name="pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="122-2584" color="#a6acb3" type="standard"/></Feature><Feature recentID="15" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2609-2627" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2610-2610" color="#a6acb3" type="standard"/></Feature><Feature recentID="13" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-121" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="12" name="FTL_0125" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="72-117" color="#00e600" type="standard"/></Feature><Feature recentID="11" name="hypothetical protein" directionality="2" translationMW="1785.21" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="28" maxFusedRunOn="28"><Segment range="72-117" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="pdpB" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-117" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2666" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2665-2665" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2667-3152" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2703-3152" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2703-3152" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2774-2793" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="2854-2874" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2667-2667" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3153-3202" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P823 DFPI_FR1R2" sequence="atatGCGGCCGCtgctatcattgtctacttaattagaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2574-2602" boundStrand="0" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="2574-2602" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite><BindingSite simplified="1" location="2574-2602" boundStrand="0" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="2574-2602" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite></Primer><Primer recentID="1" name="KROL311  pdpA_qPCR_R1" sequence="cacttgatctttgtatcgcactatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2133-2157" boundStrand="0" annealedBases="cacttgatctttgtatcgcactatc" meltingTemperature="57"><Component hybridizedRange="2133-2157" bases="cacttgatctttgtatcgcactatc"/></BindingSite><BindingSite simplified="1" location="2133-2157" boundStrand="0" annealedBases="cacttgatctttgtatcgcactatc" meltingTemperature="57"><Component hybridizedRange="2133-2157" bases="cacttgatctttgtatcgcactatc"/></BindingSite></Primer><Primer recentID="2" name="KROL310  pdpA_qPCR_F1" sequence="cttgagctccctcatgattaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2190-2211" boundStrand="1" annealedBases="cttgagctccctcatgattaca" meltingTemperature="57"><Component hybridizedRange="2190-2211" bases="cttgagctccctcatgattaca"/></BindingSite><BindingSite simplified="1" location="2190-2211" boundStrand="1" annealedBases="cttgagctccctcatgattaca" meltingTemperature="57"><Component hybridizedRange="2190-2211" bases="cttgagctccctcatgattaca"/></BindingSite></Primer><Primer recentID="3" name="KROL312  pdpA_qPCR_F2" sequence="aatcatgagggagctcaagaatta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2193-2216" boundStrand="0" annealedBases="aatcatgagggagctcaagaatta" meltingTemperature="55"><Component hybridizedRange="2193-2216" bases="aatcatgagggagctcaagaatta"/></BindingSite><BindingSite simplified="1" location="2193-2216" boundStrand="0" annealedBases="aatcatgagggagctcaagaatta" meltingTemperature="55"><Component hybridizedRange="2193-2216" bases="aatcatgagggagctcaagaatta"/></BindingSite></Primer><Primer recentID="4" name="KROL313  pdpA_qPCR_R2" sequence="cgacaaactataaagactct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2269-2288" boundStrand="1" annealedBases="cgacaaactataaagactct" meltingTemperature="50"><Component hybridizedRange="2269-2288" bases="cgacaaactataaagactct"/></BindingSite><BindingSite simplified="1" location="2269-2288" boundStrand="1" annealedBases="cgacaaactataaagactct" meltingTemperature="50"><Component hybridizedRange="2269-2288" bases="cgacaaactataaagactct"/></BindingSite></Primer><Primer recentID="5" name="KROL314  pdpA_qPCR_F3" sequence="acccaatatatcgtgaaaggct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2067-2088" boundStrand="0" annealedBases="acccaatatatcgtgaaaggct" meltingTemperature="56"><Component hybridizedRange="2067-2088" bases="acccaatatatcgtgaaaggct"/></BindingSite><BindingSite simplified="1" location="2067-2088" boundStrand="0" annealedBases="acccaatatatcgtgaaaggct" meltingTemperature="56"><Component hybridizedRange="2067-2088" bases="acccaatatatcgtgaaaggct"/></BindingSite></Primer><Primer recentID="6" name="KROL315  pdpA_qPCR_R3" sequence="gatagtgcgatacaaagatcaagtg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2133-2157" boundStrand="1" annealedBases="gatagtgcgatacaaagatcaagtg" meltingTemperature="56"><Component hybridizedRange="2133-2157" bases="gatagtgcgatacaaagatcaagtg"/></BindingSite><BindingSite simplified="1" location="2133-2157" boundStrand="1" annealedBases="gatagtgcgatacaaagatcaagtg" meltingTemperature="56"><Component hybridizedRange="2133-2157" bases="gatagtgcgatacaaagatcaagtg"/></BindingSite></Primer><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="2773-2792" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="2773-2792" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="2773-2792" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="2773-2792" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="2853-2873" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="2853-2873" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="2853-2873" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="2853-2873" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:44:19">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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6)l=Ţį!Z*Z85_̽ekX[gܯIɷG^^n`O9ME{	6yrjqBH,)3`̥dC    # (  %g    YZ     1             9=UGC@)@o֍8e|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_90l   D   I   R   T   ~    7zXZ  ִF !   t/t] 3D].?I*ә_.ZvќP,Vj,ު~mC<ґ8hlFOlY.k(DW`!
 	KJ9rEPܤ$FcprMUq3+Xfg=Gj6F#5Ɇca>48r7Nn2h݊r3u>ɧ&[_ 9o)b`.5·QhxOAd)9"qOG`yPlqsLu	j3^񋘢UnMM]=*a/aY P Vc(8Z/tMA-3"Ietih7eewH2Uj" V򋷹bg*xV<e﷍iYL bw&r,<K?G-#3iU
)K WqijcR.Z-уVtڡgۖXj%üx"]|pF+epbm<Ө7#<=5­ְ~zAw7pᙇ⭷5-oq2iM:%9*ݬOqcX8rjhWҟGs\/&Gݡ/x:lSQƈS7ĕӟrz<3V(9yiVpjfIWZॗ-KǗܹ~(M*wixɻ41Goy6@QAp>WeFzʅy[fHCtFXEV_[yuG"|+`V\q0Nw
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%[uctIU#(W	^W?_0x{/]w`[MIB LIolY.W5R]3"$p@^$!?& )ʲa:lê>$dp2d]$Sfv{y7-BkV  /8 /  qWg    YZ     0             9=UGC@)@o֍8e|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    D   I   R   T   ~    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="81-87" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="15-62" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="108-126" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="125-125" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-612" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="163-612" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="163-612" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-253" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="314-334" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-127" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="613-662" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="233-252" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="233-252" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="233-252" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="233-252" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="313-333" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="313-333" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="313-333" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="313-333" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:47:54">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     /             Uʑ 9Z4b	gdYUIUVZSmlЩ18{);NFxӞxMN8nS׍TY^eYT׉gYeF`E
#gS֞RcQqz^%Ҕ%$RNXQIl놵fZJUU;   ;   @   I   K   u    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-78" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="99-117" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="116-116" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="118-603" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="154-603" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="154-603" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="225-244" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="305-325" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="118-118" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="604-653" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="224-243" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="224-243" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="224-243" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="224-243" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="304-324" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="304-324" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="304-324" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="304-324" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:47:10">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     .             Uʑ 9Z4ᙋ'륜{yf!fVA&aUYiNꛩB@ cU:ӕ;GNy58㉹O^5RRezAeS^&gMePR%uC+aNZyIEUxJSP^	H9bJuE%VjZ՚j*UU`C$   ;   @   I   J   t    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-77" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-116" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-115" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="117-602" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="153-602" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="153-602" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="224-243" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="304-324" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="117-117" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="603-652" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="223-242" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="223-242" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="223-242" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="223-242" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="303-323" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="303-323" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="303-323" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="303-323" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:33">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     -             Uʑ 9Z4ᘱ|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   r    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-75" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="96-114" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-113" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-600" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="151-600" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="151-600" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="222-241" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="302-322" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-115" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-650" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="221-240" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="221-240" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="221-240" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="221-240" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="301-321" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="301-321" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="301-321" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="301-321" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:28">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     ,             Uʑ 9Z4|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   n    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  X<?xml version="1.0"?><Features recycledIDs="10,11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="92-110" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="109-109" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="111-596" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="147-596" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="147-596" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="218-237" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="298-318" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="111-111" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="597-646" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="217-236" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="217-236" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="217-236" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="217-236" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="297-317" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="297-317" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="297-317" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="297-317" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:14">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  13   +  Q          Uʑ 9Z4JHVYaUjUgꩶTZjzbފj*E($4Ji{Yjߩ%Qj֔e-ehijbD%&aaeھZ&QOdI}VƄ냦ZQje:][)}TzzAJFe؋G&MjfrީiJ(IVY:fS>U*.JQ%IEDnվ$Z&m:th`a&[H)RݴvU*i4ednjqKinud=mYVM;RUVU^$VV: ɪiEUTf۔ꚕ9F
/T -h{Ev	YkTY*=`UnUNmYe
dKmT<떪aR%)f(gUJjC%4flCNZ*yJIGmj@NZWJZZ1.JYeknb[sYj>Rnj(IibZYfNijM^EBڙzJ^Zb虩[Jf9v^ɤ|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   G  
/  
j    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="21"><Feature recentID="20" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="122-2608" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="19" name="FTL_0126" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="122-2584" color="#00e600" type="standard"/></Feature><Feature recentID="18" name="hypothetical protein" directionality="2" translationMW="95341.69" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="122-2584" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2608-2608" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="16" name="pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="122-2584" color="#a6acb3" type="standard"/></Feature><Feature recentID="15" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2609-2627" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2610-2610" color="#a6acb3" type="standard"/></Feature><Feature recentID="13" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-121" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="12" name="FTL_0125" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="72-117" color="#00e600" type="standard"/></Feature><Feature recentID="11" name="hypothetical protein" directionality="2" translationMW="1785.21" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="28" maxFusedRunOn="28"><Segment range="72-117" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="pdpB" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-117" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2666" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2665-2665" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2667-3152" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2703-3152" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2703-3152" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2774-2793" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="2854-2874" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2667-2667" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3153-3202" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P823 DFPI_FR1R2" sequence="atatGCGGCCGCtgctatcattgtctacttaattagaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2574-2602" boundStrand="0" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="2574-2602" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite><BindingSite simplified="1" location="2574-2602" boundStrand="0" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="2574-2602" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite></Primer><Primer recentID="1" name="KROL311  pdpA_qPCR_R1" sequence="cacttgatctttgtatcgcactatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2133-2157" boundStrand="0" annealedBases="cacttgatctttgtatcgcactatc" meltingTemperature="57"><Component hybridizedRange="2133-2157" bases="cacttgatctttgtatcgcactatc"/></BindingSite><BindingSite simplified="1" location="2133-2157" boundStrand="0" annealedBases="cacttgatctttgtatcgcactatc" meltingTemperature="57"><Component hybridizedRange="2133-2157" bases="cacttgatctttgtatcgcactatc"/></BindingSite></Primer><Primer recentID="2" name="KROL310  pdpA_qPCR_F1" sequence="cttgagctccctcatgattaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2190-2211" boundStrand="1" annealedBases="cttgagctccctcatgattaca" meltingTemperature="57"><Component hybridizedRange="2190-2211" bases="cttgagctccctcatgattaca"/></BindingSite><BindingSite simplified="1" location="2190-2211" boundStrand="1" annealedBases="cttgagctccctcatgattaca" meltingTemperature="57"><Component hybridizedRange="2190-2211" bases="cttgagctccctcatgattaca"/></BindingSite></Primer><Primer recentID="3" name="KROL312  pdpA_qPCR_F2" sequence="aatcatgagggagctcaagaatta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2193-2216" boundStrand="0" annealedBases="aatcatgagggagctcaagaatta" meltingTemperature="55"><Component hybridizedRange="2193-2216" bases="aatcatgagggagctcaagaatta"/></BindingSite><BindingSite simplified="1" location="2193-2216" boundStrand="0" annealedBases="aatcatgagggagctcaagaatta" meltingTemperature="55"><Component hybridizedRange="2193-2216" bases="aatcatgagggagctcaagaatta"/></BindingSite></Primer><Primer recentID="4" name="KROL313  pdpA_qPCR_R2" sequence="cgacaaactataaagactct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2269-2288" boundStrand="1" annealedBases="cgacaaactataaagactct" meltingTemperature="50"><Component hybridizedRange="2269-2288" bases="cgacaaactataaagactct"/></BindingSite><BindingSite simplified="1" location="2269-2288" boundStrand="1" annealedBases="cgacaaactataaagactct" meltingTemperature="50"><Component hybridizedRange="2269-2288" bases="cgacaaactataaagactct"/></BindingSite></Primer><Primer recentID="5" name="KROL314  pdpA_qPCR_F3" sequence="acccaatatatcgtgaaaggct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2067-2088" boundStrand="0" annealedBases="acccaatatatcgtgaaaggct" meltingTemperature="56"><Component hybridizedRange="2067-2088" bases="acccaatatatcgtgaaaggct"/></BindingSite><BindingSite simplified="1" location="2067-2088" boundStrand="0" annealedBases="acccaatatatcgtgaaaggct" meltingTemperature="56"><Component hybridizedRange="2067-2088" bases="acccaatatatcgtgaaaggct"/></BindingSite></Primer><Primer recentID="6" name="KROL315  pdpA_qPCR_R3" sequence="gatagtgcgatacaaagatcaagtg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2133-2157" boundStrand="1" annealedBases="gatagtgcgatacaaagatcaagtg" meltingTemperature="56"><Component hybridizedRange="2133-2157" bases="gatagtgcgatacaaagatcaagtg"/></BindingSite><BindingSite simplified="1" location="2133-2157" boundStrand="1" annealedBases="gatagtgcgatacaaagatcaagtg" meltingTemperature="56"><Component hybridizedRange="2133-2157" bases="gatagtgcgatacaaagatcaagtg"/></BindingSite></Primer><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="2773-2792" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="2773-2792" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="2773-2792" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="2773-2792" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="2853-2873" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="2853-2873" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="2853-2873" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="2853-2873" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:44:19">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     >             f0󘺟waiٚ(j`q	ЖaA)ZRuPJ'Y`kYF}SaQH)+l\cZR떙@٥BKh'FudxF"Uaymz~g^
ЕFmRx^gj9_X8٥U%_vWťjU:)}&             7zXZ  ִF !   t/d] 3D].?I*ә_.ZvќP,Vj,ު~mC<ґ8hlFOlY.k(DW`!
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B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_90l   D   I   R   T   ~    7zXZ  ִF !   t/t] 3D].?I*ә_.ZvќP,Vj,ު~mC<ґ8hlFOlY.k(DW`!
 	KJ9rEPܤ$FcprMUq3+Xfg=Gj6F#5Ɇca>48r7Nn2h݊r3u>ɧ&[_ 9o)b`.5·QhxOAd)9"qOG`yPlqsLu	j3^񋘢UnMM]=*a/aY P Vc(8Z/tMA-3"Ietih7eewH2Uj" V򋷹bg*xV<e﷍iYL bw&r,<K?G-#3iU
)K WqijcR.Z-уVtڡgۖXj%üx"]|pF+epbm<Ө7#<=5­ְ~zAw7pᙇ⭷5-oq2iM:%9*ݬOqcX8rjhWҟGs\/&Gݡ/x:lSQƈS7ĕӟrz<3V(9yiVpjfIWZॗ-KǗܹ~(M*wixɻ41Goy6@QAp>WeFzʅy[fHCtFXEV_[yuG"|+`V\q0Nw
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%[uctIU#(W	^W?_0x{/]w`[MIB LIolY.W5R]3"$p@^$!?& )ʲa:lê>$dp2d]$Sfv{y7-BkV  /8 /  qWg    YZ     G             9=UGC@)@o֍8e|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    D   I   R   T   ~    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="81-87" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="15-62" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="108-126" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="125-125" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-612" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="163-612" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="163-612" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-253" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="314-334" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-127" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="613-662" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="233-252" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="233-252" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="233-252" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="233-252" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="313-333" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="313-333" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="313-333" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="313-333" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:47:54">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     F             Uʑ 9Z4b	gdYUIUVZSmlЩ18{);NFxӞxMN8nS׍TY^eYT׉gYeF`E
#gS֞RcQqz^%Ҕ%$RNXQIl놵fZJUU;   ;   @   I   K   u    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-78" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="99-117" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="116-116" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="118-603" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="154-603" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="154-603" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="225-244" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="305-325" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="118-118" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="604-653" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="224-243" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="224-243" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="224-243" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="224-243" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="304-324" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="304-324" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="304-324" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="304-324" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:47:10">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     E             Uʑ 9Z4ᙋ'륜{yf!fVA&aUYiNꛩB@ cU:ӕ;GNy58㉹O^5RRezAeS^&gMePR%uC+aNZyIEUxJSP^	H9bJuE%VjZ՚j*UU`C$   ;   @   I   J   t    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-77" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-116" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-115" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="117-602" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="153-602" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="153-602" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="224-243" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="304-324" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="117-117" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="603-652" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="223-242" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="223-242" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="223-242" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="223-242" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="303-323" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="303-323" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="303-323" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="303-323" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:33">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     D             Uʑ 9Z4ᘱ|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   r    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-75" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="96-114" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-113" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-600" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="151-600" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="151-600" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="222-241" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="302-322" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-115" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-650" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="221-240" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="221-240" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="221-240" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="221-240" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="301-321" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="301-321" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="301-321" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="301-321" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:28">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     C             Uʑ 9Z4|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   n    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  X<?xml version="1.0"?><Features recycledIDs="10,11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="92-110" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="109-109" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="111-596" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="147-596" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="147-596" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="218-237" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="298-318" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="111-111" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="597-646" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="217-236" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="217-236" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="217-236" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="217-236" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="297-317" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="297-317" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="297-317" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="297-317" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:14">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  13   B  Q          Uʑ 9Z4JHVYaUjUgꩶTZjzbފj*E($4Ji{Yjߩ%Qj֔e-ehijbD%&aaeھZ&QOdI}VƄ냦ZQje:][)}TzzAJFe؋G&MjfrީiJ(IVY:fS>U*.JQ%IEDnվ$Z&m:th`a&[H)RݴvU*i4ednjqKinud=mYVM;RUVU^$VV: ɪiEUTf۔ꚕ9F
/T -h{Ev	YkTY*=`UnUNmYe
dKmT<떪aR%)f(gUJjC%4flCNZ*yJIGmj@NZWJZZ1.JYeknb[sYj>Rnj(IibZYfNijM^EBڙzJ^Zb虩[Jf9v^ɤ|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   G  
/  
j    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="21"><Feature recentID="20" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="122-2608" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="19" name="FTL_0126" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="122-2584" color="#00e600" type="standard"/></Feature><Feature recentID="18" name="hypothetical protein" directionality="2" translationMW="95341.69" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="122-2584" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2608-2608" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="16" name="pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="122-2584" color="#a6acb3" type="standard"/></Feature><Feature recentID="15" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2609-2627" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2610-2610" color="#a6acb3" type="standard"/></Feature><Feature recentID="13" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-121" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="12" name="FTL_0125" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="72-117" color="#00e600" type="standard"/></Feature><Feature recentID="11" name="hypothetical protein" directionality="2" translationMW="1785.21" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="28" maxFusedRunOn="28"><Segment range="72-117" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="pdpB" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-117" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2666" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2665-2665" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2667-3152" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2703-3152" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2703-3152" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2774-2793" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="2854-2874" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2667-2667" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3153-3202" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P823 DFPI_FR1R2" sequence="atatGCGGCCGCtgctatcattgtctacttaattagaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2574-2602" boundStrand="0" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="2574-2602" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite><BindingSite simplified="1" location="2574-2602" boundStrand="0" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="2574-2602" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite></Primer><Primer recentID="1" name="KROL311  pdpA_qPCR_R1" sequence="cacttgatctttgtatcgcactatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2133-2157" boundStrand="0" annealedBases="cacttgatctttgtatcgcactatc" meltingTemperature="57"><Component hybridizedRange="2133-2157" bases="cacttgatctttgtatcgcactatc"/></BindingSite><BindingSite simplified="1" location="2133-2157" boundStrand="0" annealedBases="cacttgatctttgtatcgcactatc" meltingTemperature="57"><Component hybridizedRange="2133-2157" bases="cacttgatctttgtatcgcactatc"/></BindingSite></Primer><Primer recentID="2" name="KROL310  pdpA_qPCR_F1" sequence="cttgagctccctcatgattaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2190-2211" boundStrand="1" annealedBases="cttgagctccctcatgattaca" meltingTemperature="57"><Component hybridizedRange="2190-2211" bases="cttgagctccctcatgattaca"/></BindingSite><BindingSite simplified="1" location="2190-2211" boundStrand="1" annealedBases="cttgagctccctcatgattaca" meltingTemperature="57"><Component hybridizedRange="2190-2211" bases="cttgagctccctcatgattaca"/></BindingSite></Primer><Primer recentID="3" name="KROL312  pdpA_qPCR_F2" sequence="aatcatgagggagctcaagaatta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2193-2216" boundStrand="0" annealedBases="aatcatgagggagctcaagaatta" meltingTemperature="55"><Component hybridizedRange="2193-2216" bases="aatcatgagggagctcaagaatta"/></BindingSite><BindingSite simplified="1" location="2193-2216" boundStrand="0" annealedBases="aatcatgagggagctcaagaatta" meltingTemperature="55"><Component hybridizedRange="2193-2216" bases="aatcatgagggagctcaagaatta"/></BindingSite></Primer><Primer recentID="4" name="KROL313  pdpA_qPCR_R2" sequence="cgacaaactataaagactct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2269-2288" boundStrand="1" annealedBases="cgacaaactataaagactct" meltingTemperature="50"><Component hybridizedRange="2269-2288" bases="cgacaaactataaagactct"/></BindingSite><BindingSite simplified="1" location="2269-2288" boundStrand="1" annealedBases="cgacaaactataaagactct" meltingTemperature="50"><Component hybridizedRange="2269-2288" bases="cgacaaactataaagactct"/></BindingSite></Primer><Primer recentID="5" name="KROL314  pdpA_qPCR_F3" sequence="acccaatatatcgtgaaaggct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2067-2088" boundStrand="0" annealedBases="acccaatatatcgtgaaaggct" meltingTemperature="56"><Component hybridizedRange="2067-2088" bases="acccaatatatcgtgaaaggct"/></BindingSite><BindingSite simplified="1" location="2067-2088" boundStrand="0" annealedBases="acccaatatatcgtgaaaggct" meltingTemperature="56"><Component hybridizedRange="2067-2088" bases="acccaatatatcgtgaaaggct"/></BindingSite></Primer><Primer recentID="6" name="KROL315  pdpA_qPCR_R3" sequence="gatagtgcgatacaaagatcaagtg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2133-2157" boundStrand="1" annealedBases="gatagtgcgatacaaagatcaagtg" meltingTemperature="56"><Component hybridizedRange="2133-2157" bases="gatagtgcgatacaaagatcaagtg"/></BindingSite><BindingSite simplified="1" location="2133-2157" boundStrand="1" annealedBases="gatagtgcgatacaaagatcaagtg" meltingTemperature="56"><Component hybridizedRange="2133-2157" bases="gatagtgcgatacaaagatcaagtg"/></BindingSite></Primer><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="2773-2792" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="2773-2792" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="2773-2792" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="2773-2792" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="2853-2873" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="2853-2873" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="2853-2873" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="2853-2873" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:44:19">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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6)l=Ţį!Z*Z85_̽ekX[gܯIɷG^^n`O9ME{	6yrjqBH,)3`̥dC    # (  %g    YZ     R             9=UGC@)@o֍8e|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_90l   D   I   R   T   ~    7zXZ  ִF !   t/t] 3D].?I*ә_.ZvќP,Vj,ު~mC<ґ8hlFOlY.k(DW`!
 	KJ9rEPܤ$FcprMUq3+Xfg=Gj6F#5Ɇca>48r7Nn2h݊r3u>ɧ&[_ 9o)b`.5·QhxOAd)9"qOG`yPlqsLu	j3^񋘢UnMM]=*a/aY P Vc(8Z/tMA-3"Ietih7eewH2Uj" V򋷹bg*xV<e﷍iYL bw&r,<K?G-#3iU
)K WqijcR.Z-уVtڡgۖXj%üx"]|pF+epbm<Ө7#<=5­ְ~zAw7pᙇ⭷5-oq2iM:%9*ݬOqcX8rjhWҟGs\/&Gݡ/x:lSQƈS7ĕӟrz<3V(9yiVpjfIWZॗ-KǗܹ~(M*wixɻ41Goy6@QAp>WeFzʅy[fHCtFXEV_[yuG"|+`V\q0Nw
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%[uctIU#(W	^W?_0x{/]w`[MIB LIolY.W5R]3"$p@^$!?& )ʲa:lê>$dp2d]$Sfv{y7-BkV  /8 /  qWg    YZ     Q             9=UGC@)@o֍8e|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    D   I   R   T   ~    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="81-87" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="15-62" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="108-126" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="125-125" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-612" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="163-612" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="163-612" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-253" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="314-334" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-127" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="613-662" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="233-252" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="233-252" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="233-252" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="233-252" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="313-333" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="313-333" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="313-333" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="313-333" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:47:54">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     P             Uʑ 9Z4b	gdYUIUVZSmlЩ18{);NFxӞxMN8nS׍TY^eYT׉gYeF`E
#gS֞RcQqz^%Ҕ%$RNXQIl놵fZJUU;   ;   @   I   K   u    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-78" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="99-117" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="116-116" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="118-603" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="154-603" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="154-603" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="225-244" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="305-325" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="118-118" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="604-653" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="224-243" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="224-243" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="224-243" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="224-243" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="304-324" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="304-324" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="304-324" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="304-324" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:47:10">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     O             Uʑ 9Z4ᙋ'륜{yf!fVA&aUYiNꛩB@ cU:ӕ;GNy58㉹O^5RRezAeS^&gMePR%uC+aNZyIEUxJSP^	H9bJuE%VjZ՚j*UU`C$   ;   @   I   J   t    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-77" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-116" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-115" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="117-602" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="153-602" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="153-602" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="224-243" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="304-324" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="117-117" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="603-652" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="223-242" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="223-242" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="223-242" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="223-242" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="303-323" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="303-323" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="303-323" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="303-323" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:33">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     N             Uʑ 9Z4ᘱ|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   r    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-75" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="96-114" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-113" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-600" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="151-600" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="151-600" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="222-241" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="302-322" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-115" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-650" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="221-240" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="221-240" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="221-240" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="221-240" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="301-321" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="301-321" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="301-321" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="301-321" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:28">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     M             Uʑ 9Z4|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   n    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  X<?xml version="1.0"?><Features recycledIDs="10,11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="92-110" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="109-109" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="111-596" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="147-596" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="147-596" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="218-237" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="298-318" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="111-111" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="597-646" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="217-236" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="217-236" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="217-236" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="217-236" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="297-317" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="297-317" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="297-317" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="297-317" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:14">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  13   L  Q          Uʑ 9Z4JHVYaUjUgꩶTZjzbފj*E($4Ji{Yjߩ%Qj֔e-ehijbD%&aaeھZ&QOdI}VƄ냦ZQje:][)}TzzAJFe؋G&MjfrީiJ(IVY:fS>U*.JQ%IEDnվ$Z&m:th`a&[H)RݴvU*i4ednjqKinud=mYVM;RUVU^$VV: ɪiEUTf۔ꚕ9F
/T -h{Ev	YkTY*=`UnUNmYe
dKmT<떪aR%)f(gUJjC%4flCNZ*yJIGmj@NZWJZZ1.JYeknb[sYj>Rnj(IibZYfNijM^EBڙzJ^Zb虩[Jf9v^ɤ|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   G  
/  
j    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="21"><Feature recentID="20" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="122-2608" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="19" name="FTL_0126" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="122-2584" color="#00e600" type="standard"/></Feature><Feature recentID="18" name="hypothetical protein" directionality="2" translationMW="95341.69" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="122-2584" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2608-2608" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="16" name="pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="122-2584" color="#a6acb3" type="standard"/></Feature><Feature recentID="15" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2609-2627" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2610-2610" color="#a6acb3" type="standard"/></Feature><Feature recentID="13" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-121" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="12" name="FTL_0125" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="72-117" color="#00e600" type="standard"/></Feature><Feature recentID="11" name="hypothetical protein" directionality="2" translationMW="1785.21" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="28" maxFusedRunOn="28"><Segment range="72-117" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="pdpB" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-117" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2666" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2665-2665" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2667-3152" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2703-3152" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2703-3152" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2774-2793" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="2854-2874" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2667-2667" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3153-3202" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P823 DFPI_FR1R2" sequence="atatGCGGCCGCtgctatcattgtctacttaattagaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2574-2602" boundStrand="0" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="2574-2602" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite><BindingSite simplified="1" location="2574-2602" boundStrand="0" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="2574-2602" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite></Primer><Primer recentID="1" name="KROL311  pdpA_qPCR_R1" sequence="cacttgatctttgtatcgcactatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2133-2157" boundStrand="0" annealedBases="cacttgatctttgtatcgcactatc" meltingTemperature="57"><Component hybridizedRange="2133-2157" bases="cacttgatctttgtatcgcactatc"/></BindingSite><BindingSite simplified="1" location="2133-2157" boundStrand="0" annealedBases="cacttgatctttgtatcgcactatc" meltingTemperature="57"><Component hybridizedRange="2133-2157" bases="cacttgatctttgtatcgcactatc"/></BindingSite></Primer><Primer recentID="2" name="KROL310  pdpA_qPCR_F1" sequence="cttgagctccctcatgattaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2190-2211" boundStrand="1" annealedBases="cttgagctccctcatgattaca" meltingTemperature="57"><Component hybridizedRange="2190-2211" bases="cttgagctccctcatgattaca"/></BindingSite><BindingSite simplified="1" location="2190-2211" boundStrand="1" annealedBases="cttgagctccctcatgattaca" meltingTemperature="57"><Component hybridizedRange="2190-2211" bases="cttgagctccctcatgattaca"/></BindingSite></Primer><Primer recentID="3" name="KROL312  pdpA_qPCR_F2" sequence="aatcatgagggagctcaagaatta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2193-2216" boundStrand="0" annealedBases="aatcatgagggagctcaagaatta" meltingTemperature="55"><Component hybridizedRange="2193-2216" bases="aatcatgagggagctcaagaatta"/></BindingSite><BindingSite simplified="1" location="2193-2216" boundStrand="0" annealedBases="aatcatgagggagctcaagaatta" meltingTemperature="55"><Component hybridizedRange="2193-2216" bases="aatcatgagggagctcaagaatta"/></BindingSite></Primer><Primer recentID="4" name="KROL313  pdpA_qPCR_R2" sequence="cgacaaactataaagactct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2269-2288" boundStrand="1" annealedBases="cgacaaactataaagactct" meltingTemperature="50"><Component hybridizedRange="2269-2288" bases="cgacaaactataaagactct"/></BindingSite><BindingSite simplified="1" location="2269-2288" boundStrand="1" annealedBases="cgacaaactataaagactct" meltingTemperature="50"><Component hybridizedRange="2269-2288" bases="cgacaaactataaagactct"/></BindingSite></Primer><Primer recentID="5" name="KROL314  pdpA_qPCR_F3" sequence="acccaatatatcgtgaaaggct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2067-2088" boundStrand="0" annealedBases="acccaatatatcgtgaaaggct" meltingTemperature="56"><Component hybridizedRange="2067-2088" bases="acccaatatatcgtgaaaggct"/></BindingSite><BindingSite simplified="1" location="2067-2088" boundStrand="0" annealedBases="acccaatatatcgtgaaaggct" meltingTemperature="56"><Component hybridizedRange="2067-2088" bases="acccaatatatcgtgaaaggct"/></BindingSite></Primer><Primer recentID="6" name="KROL315  pdpA_qPCR_R3" sequence="gatagtgcgatacaaagatcaagtg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2133-2157" boundStrand="1" annealedBases="gatagtgcgatacaaagatcaagtg" meltingTemperature="56"><Component hybridizedRange="2133-2157" bases="gatagtgcgatacaaagatcaagtg"/></BindingSite><BindingSite simplified="1" location="2133-2157" boundStrand="1" annealedBases="gatagtgcgatacaaagatcaagtg" meltingTemperature="56"><Component hybridizedRange="2133-2157" bases="gatagtgcgatacaaagatcaagtg"/></BindingSite></Primer><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="2773-2792" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="2773-2792" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="2773-2792" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="2773-2792" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="2853-2873" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="2853-2873" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="2853-2873" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="2853-2873" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:44:19">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     _             f0󘺟waiٚ(j`q	ЖaA)ZRuPJ'Y`kYF}SaQH)+l\cZR떙@٥BKh'FudxF"Uaymz~g^
ЕFmRx^gj9_X8٥U%_vWťjU:)}&             7zXZ  ִF !   t/d] 3D].?I*ә_.ZvќP,Vj,ު~mC<ґ8hlFOlY.k(DW`!
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6)l=Ţį!Z*Z85_̽ekX[gܯIɷG^^n`O9ME{	6yrjqBH,)3`̥dC    # (  %g    YZ                  9=UGC@)@o֍8e|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_90l   D   I   R   T   ~    7zXZ  ִF !   t/t] 3D].?I*ә_.ZvќP,Vj,ު~mC<ґ8hlFOlY.k(DW`!
 	KJ9rEPܤ$FcprMUq3+Xfg=Gj6F#5Ɇca>48r7Nn2h݊r3u>ɧ&[_ 9o)b`.5·QhxOAd)9"qOG`yPlqsLu	j3^񋘢UnMM]=*a/aY P Vc(8Z/tMA-3"Ietih7eewH2Uj" V򋷹bg*xV<e﷍iYL bw&r,<K?G-#3iU
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%[uctIU#(W	^W?_0x{/]w`[MIB LIolY.W5R]3"$p@^$!?& )ʲa:lê>$dp2d]$Sfv{y7-BkV  /8 /  qWg    YZ                  9=UGC@)@o֍8e|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    D   I   R   T   ~    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="81-87" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="15-62" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="108-126" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="125-125" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-612" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="163-612" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="163-612" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-253" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="314-334" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-127" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="613-662" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="233-252" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="233-252" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="233-252" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="233-252" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="313-333" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="313-333" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="313-333" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="313-333" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:47:54">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  Uʑ 9Z4b	gdYUIUVZSmlЩ18{);NFxӞxMN8nS׍TY^eYT׉gYeF`E
#gS֞RcQqz^%Ҕ%$RNXQIl놵fZJUU;   ;   @   I   K   u    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-78" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="99-117" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="116-116" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="118-603" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="154-603" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="154-603" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="225-244" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="305-325" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="118-118" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="604-653" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="224-243" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="224-243" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="224-243" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="224-243" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="304-324" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="304-324" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="304-324" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="304-324" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:47:10">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  Uʑ 9Z4ᙋ'륜{yf!fVA&aUYiNꛩB@ cU:ӕ;GNy58㉹O^5RRezAeS^&gMePR%uC+aNZyIEUxJSP^	H9bJuE%VjZ՚j*UU`C$   ;   @   I   J   t    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-77" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-116" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-115" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="117-602" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="153-602" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="153-602" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="224-243" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="304-324" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="117-117" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="603-652" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="223-242" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="223-242" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="223-242" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="223-242" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="303-323" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="303-323" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="303-323" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="303-323" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:33">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  Uʑ 9Z4ᘱ|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   r    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-75" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="96-114" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-113" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-600" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="151-600" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="151-600" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="222-241" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="302-322" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-115" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-650" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="221-240" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="221-240" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="221-240" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="221-240" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="301-321" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="301-321" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="301-321" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="301-321" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:28">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  Uʑ 9Z4|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   n    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  X<?xml version="1.0"?><Features recycledIDs="10,11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="92-110" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="109-109" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="111-596" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="147-596" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="147-596" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="218-237" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="298-318" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="111-111" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="597-646" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="217-236" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="217-236" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="217-236" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="217-236" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="297-317" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="297-317" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="297-317" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="297-317" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:14">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  13     Q          Uʑ 9Z4JHVYaUjUgꩶTZjzbފj*E($4Ji{Yjߩ%Qj֔e-ehijbD%&aaeھZ&QOdI}VƄ냦ZQje:][)}TzzAJFe؋G&MjfrީiJ(IVY:fS>U*.JQ%IEDnվ$Z&m:th`a&[H)RݴvU*i4ednjqKinud=mYVM;RUVU^$VV: ɪiEUTf۔ꚕ9F
/T -h{Ev	YkTY*=`UnUNmYe
dKmT<떪aR%)f(gUJjC%4flCNZ*yJIGmj@NZWJZZ1.JYeknb[sYj>Rnj(IibZYfNijM^EBڙzJ^Zb虩[Jf9v^ɤ|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   G  
/  
j    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="21"><Feature recentID="20" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="122-2608" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="19" name="FTL_0126" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="122-2584" color="#00e600" type="standard"/></Feature><Feature recentID="18" name="hypothetical protein" directionality="2" translationMW="95341.69" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="122-2584" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2608-2608" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="16" name="pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="122-2584" color="#a6acb3" type="standard"/></Feature><Feature recentID="15" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2609-2627" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2610-2610" color="#a6acb3" type="standard"/></Feature><Feature recentID="13" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-121" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="12" name="FTL_0125" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="72-117" color="#00e600" type="standard"/></Feature><Feature recentID="11" name="hypothetical protein" directionality="2" translationMW="1785.21" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="28" maxFusedRunOn="28"><Segment range="72-117" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="pdpB" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-117" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2666" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2665-2665" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2667-3152" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2703-3152" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2703-3152" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2774-2793" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="2854-2874" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2667-2667" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3153-3202" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P823 DFPI_FR1R2" sequence="atatGCGGCCGCtgctatcattgtctacttaattagaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2574-2602" boundStrand="0" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="2574-2602" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite><BindingSite simplified="1" location="2574-2602" boundStrand="0" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="2574-2602" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite></Primer><Primer recentID="1" name="KROL311  pdpA_qPCR_R1" sequence="cacttgatctttgtatcgcactatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2133-2157" boundStrand="0" annealedBases="cacttgatctttgtatcgcactatc" meltingTemperature="57"><Component hybridizedRange="2133-2157" bases="cacttgatctttgtatcgcactatc"/></BindingSite><BindingSite simplified="1" location="2133-2157" boundStrand="0" annealedBases="cacttgatctttgtatcgcactatc" meltingTemperature="57"><Component hybridizedRange="2133-2157" bases="cacttgatctttgtatcgcactatc"/></BindingSite></Primer><Primer recentID="2" name="KROL310  pdpA_qPCR_F1" sequence="cttgagctccctcatgattaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2190-2211" boundStrand="1" annealedBases="cttgagctccctcatgattaca" meltingTemperature="57"><Component hybridizedRange="2190-2211" bases="cttgagctccctcatgattaca"/></BindingSite><BindingSite simplified="1" location="2190-2211" boundStrand="1" annealedBases="cttgagctccctcatgattaca" meltingTemperature="57"><Component hybridizedRange="2190-2211" bases="cttgagctccctcatgattaca"/></BindingSite></Primer><Primer recentID="3" name="KROL312  pdpA_qPCR_F2" sequence="aatcatgagggagctcaagaatta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2193-2216" boundStrand="0" annealedBases="aatcatgagggagctcaagaatta" meltingTemperature="55"><Component hybridizedRange="2193-2216" bases="aatcatgagggagctcaagaatta"/></BindingSite><BindingSite simplified="1" location="2193-2216" boundStrand="0" annealedBases="aatcatgagggagctcaagaatta" meltingTemperature="55"><Component hybridizedRange="2193-2216" bases="aatcatgagggagctcaagaatta"/></BindingSite></Primer><Primer recentID="4" name="KROL313  pdpA_qPCR_R2" sequence="cgacaaactataaagactct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2269-2288" boundStrand="1" annealedBases="cgacaaactataaagactct" meltingTemperature="50"><Component hybridizedRange="2269-2288" bases="cgacaaactataaagactct"/></BindingSite><BindingSite simplified="1" location="2269-2288" boundStrand="1" annealedBases="cgacaaactataaagactct" meltingTemperature="50"><Component hybridizedRange="2269-2288" bases="cgacaaactataaagactct"/></BindingSite></Primer><Primer recentID="5" name="KROL314  pdpA_qPCR_F3" sequence="acccaatatatcgtgaaaggct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2067-2088" boundStrand="0" annealedBases="acccaatatatcgtgaaaggct" meltingTemperature="56"><Component hybridizedRange="2067-2088" bases="acccaatatatcgtgaaaggct"/></BindingSite><BindingSite simplified="1" location="2067-2088" boundStrand="0" annealedBases="acccaatatatcgtgaaaggct" meltingTemperature="56"><Component hybridizedRange="2067-2088" bases="acccaatatatcgtgaaaggct"/></BindingSite></Primer><Primer recentID="6" name="KROL315  pdpA_qPCR_R3" sequence="gatagtgcgatacaaagatcaagtg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2133-2157" boundStrand="1" annealedBases="gatagtgcgatacaaagatcaagtg" meltingTemperature="56"><Component hybridizedRange="2133-2157" bases="gatagtgcgatacaaagatcaagtg"/></BindingSite><BindingSite simplified="1" location="2133-2157" boundStrand="1" annealedBases="gatagtgcgatacaaagatcaagtg" meltingTemperature="56"><Component hybridizedRange="2133-2157" bases="gatagtgcgatacaaagatcaagtg"/></BindingSite></Primer><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="2773-2792" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="2773-2792" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="2773-2792" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="2773-2792" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="2853-2873" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="2853-2873" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="2853-2873" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="2853-2873" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:44:19">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
     	  p          9 OU_QP
cN)|,_	w%M|
G+,a)'\!_1|RKb	w}|4z _W^Fn}r,j|GEs	tWBDQ]E/B0s\R!W69v^ɤ|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_90 Î]SPS|5e\ Ao:x{8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%ˡ/_QzlM=A1	#9EI&;TJ	;5u}L FUE sqWFPnoVXR[[&fWt:[HC~nJ>,yp:}?8jm5P<LH/zϽ.ihS%$5(t6"철:Ӽ+1)o#US
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%[uctIU#(W	^W?_0x{/]w`[MIB LIolY.W5R]3"$p@^$!?& )ʲa:lê>$dp2d]$Sfv{y7-BkV  /8 /  qWg    YZ                  9=UGC@)@o֍8e|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    D   I   R   T   ~    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="81-87" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="15-62" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="108-126" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="125-125" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-612" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="163-612" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="163-612" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-253" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="314-334" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-127" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="613-662" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="233-252" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="233-252" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="233-252" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="233-252" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="313-333" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="313-333" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="313-333" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="313-333" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:47:54">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  Uʑ 9Z4b	gdYUIUVZSmlЩ18{);NFxӞxMN8nS׍TY^eYT׉gYeF`E
#gS֞RcQqz^%Ҕ%$RNXQIl놵fZJUU;   ;   @   I   K   u    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-78" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="99-117" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="116-116" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="118-603" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="154-603" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="154-603" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="225-244" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="305-325" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="118-118" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="604-653" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="224-243" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="224-243" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="224-243" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="224-243" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="304-324" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="304-324" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="304-324" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="304-324" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:47:10">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  Uʑ 9Z4ᙋ'륜{yf!fVA&aUYiNꛩB@ cU:ӕ;GNy58㉹O^5RRezAeS^&gMePR%uC+aNZyIEUxJSP^	H9bJuE%VjZ՚j*UU`C$   ;   @   I   J   t    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-77" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-116" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-115" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="117-602" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="153-602" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="153-602" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="224-243" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="304-324" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="117-117" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="603-652" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="223-242" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="223-242" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="223-242" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="223-242" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="303-323" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="303-323" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="303-323" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="303-323" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:33">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  Uʑ 9Z4ᘱ|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   r    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-75" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="96-114" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-113" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-600" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="151-600" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="151-600" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="222-241" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="302-322" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-115" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-650" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="221-240" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="221-240" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="221-240" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="221-240" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="301-321" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="301-321" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="301-321" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="301-321" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:28">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  Uʑ 9Z4|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   n    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  X<?xml version="1.0"?><Features recycledIDs="10,11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="92-110" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="109-109" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="111-596" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="147-596" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="147-596" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="218-237" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="298-318" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="111-111" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="597-646" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="217-236" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="217-236" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="217-236" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="217-236" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="297-317" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="297-317" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="297-317" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="297-317" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:14">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  13     Q          Uʑ 9Z4JHVYaUjUgꩶTZjzbފj*E($4Ji{Yjߩ%Qj֔e-ehijbD%&aaeھZ&QOdI}VƄ냦ZQje:][)}TzzAJFe؋G&MjfrީiJ(IVY:fS>U*.JQ%IEDnվ$Z&m:th`a&[H)RݴvU*i4ednjqKinud=mYVM;RUVU^$VV: ɪiEUTf۔ꚕ9F
/T -h{Ev	YkTY*=`UnUNmYe
dKmT<떪aR%)f(gUJjC%4flCNZ*yJIGmj@NZWJZZ1.JYeknb[sYj>Rnj(IibZYfNijM^EBڙzJ^Zb虩[Jf9v^ɤ|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   G  
/  
j    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="21"><Feature recentID="20" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="122-2608" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="19" name="FTL_0126" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="122-2584" color="#00e600" type="standard"/></Feature><Feature recentID="18" name="hypothetical protein" directionality="2" translationMW="95341.69" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="122-2584" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2608-2608" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="16" name="pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="122-2584" color="#a6acb3" type="standard"/></Feature><Feature recentID="15" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2609-2627" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2610-2610" color="#a6acb3" type="standard"/></Feature><Feature recentID="13" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-121" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="12" name="FTL_0125" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="72-117" color="#00e600" type="standard"/></Feature><Feature recentID="11" name="hypothetical protein" directionality="2" translationMW="1785.21" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="28" maxFusedRunOn="28"><Segment range="72-117" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="pdpB" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-117" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2666" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2665-2665" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2667-3152" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2703-3152" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2703-3152" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2774-2793" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="2854-2874" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2667-2667" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3153-3202" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P823 DFPI_FR1R2" sequence="atatGCGGCCGCtgctatcattgtctacttaattagaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2574-2602" boundStrand="0" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="2574-2602" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite><BindingSite simplified="1" location="2574-2602" boundStrand="0" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="2574-2602" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite></Primer><Primer recentID="1" name="KROL311  pdpA_qPCR_R1" sequence="cacttgatctttgtatcgcactatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2133-2157" boundStrand="0" annealedBases="cacttgatctttgtatcgcactatc" meltingTemperature="57"><Component hybridizedRange="2133-2157" bases="cacttgatctttgtatcgcactatc"/></BindingSite><BindingSite simplified="1" location="2133-2157" boundStrand="0" annealedBases="cacttgatctttgtatcgcactatc" meltingTemperature="57"><Component hybridizedRange="2133-2157" bases="cacttgatctttgtatcgcactatc"/></BindingSite></Primer><Primer recentID="2" name="KROL310  pdpA_qPCR_F1" sequence="cttgagctccctcatgattaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2190-2211" boundStrand="1" annealedBases="cttgagctccctcatgattaca" meltingTemperature="57"><Component hybridizedRange="2190-2211" bases="cttgagctccctcatgattaca"/></BindingSite><BindingSite simplified="1" location="2190-2211" boundStrand="1" annealedBases="cttgagctccctcatgattaca" meltingTemperature="57"><Component hybridizedRange="2190-2211" bases="cttgagctccctcatgattaca"/></BindingSite></Primer><Primer recentID="3" name="KROL312  pdpA_qPCR_F2" sequence="aatcatgagggagctcaagaatta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2193-2216" boundStrand="0" annealedBases="aatcatgagggagctcaagaatta" meltingTemperature="55"><Component hybridizedRange="2193-2216" bases="aatcatgagggagctcaagaatta"/></BindingSite><BindingSite simplified="1" location="2193-2216" boundStrand="0" annealedBases="aatcatgagggagctcaagaatta" meltingTemperature="55"><Component hybridizedRange="2193-2216" bases="aatcatgagggagctcaagaatta"/></BindingSite></Primer><Primer recentID="4" name="KROL313  pdpA_qPCR_R2" sequence="cgacaaactataaagactct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2269-2288" boundStrand="1" annealedBases="cgacaaactataaagactct" meltingTemperature="50"><Component hybridizedRange="2269-2288" bases="cgacaaactataaagactct"/></BindingSite><BindingSite simplified="1" location="2269-2288" boundStrand="1" annealedBases="cgacaaactataaagactct" meltingTemperature="50"><Component hybridizedRange="2269-2288" bases="cgacaaactataaagactct"/></BindingSite></Primer><Primer recentID="5" name="KROL314  pdpA_qPCR_F3" sequence="acccaatatatcgtgaaaggct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2067-2088" boundStrand="0" annealedBases="acccaatatatcgtgaaaggct" meltingTemperature="56"><Component hybridizedRange="2067-2088" bases="acccaatatatcgtgaaaggct"/></BindingSite><BindingSite simplified="1" location="2067-2088" boundStrand="0" annealedBases="acccaatatatcgtgaaaggct" meltingTemperature="56"><Component hybridizedRange="2067-2088" bases="acccaatatatcgtgaaaggct"/></BindingSite></Primer><Primer recentID="6" name="KROL315  pdpA_qPCR_R3" sequence="gatagtgcgatacaaagatcaagtg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2133-2157" boundStrand="1" annealedBases="gatagtgcgatacaaagatcaagtg" meltingTemperature="56"><Component hybridizedRange="2133-2157" bases="gatagtgcgatacaaagatcaagtg"/></BindingSite><BindingSite simplified="1" location="2133-2157" boundStrand="1" annealedBases="gatagtgcgatacaaagatcaagtg" meltingTemperature="56"><Component hybridizedRange="2133-2157" bases="gatagtgcgatacaaagatcaagtg"/></BindingSite></Primer><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="2773-2792" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="2773-2792" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="2773-2792" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="2773-2792" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="2853-2873" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="2853-2873" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="2853-2873" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="2853-2873" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:44:19">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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3͢/p@γy+/׏&"-S)C(}ϭ5̢I@}lz9gIZEĖ6 ;a    A 8  Jg    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="12"><Feature recentID="0" name="tul4-UTR" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="634-669" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="670-688" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="671-671" color="#a6acb3" type="standard"/></Feature><Feature recentID="3" name="FTL_0421" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="616-633" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="tul4" directionality="2" translationMW="760.05" type="CDS" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="82" maxFusedRunOn="82"><Segment range="616-633" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MKKIIK"/></Q></Feature><Feature recentID="5" name="Possible TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="669-669" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="2608" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="1817-2608" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="2609-2677" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="7" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1057-1645" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2678-2782" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="T7 terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="792-839" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="16-63" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="NLuc" directionality="2" translationMW="19250.08" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="82" maxFusedRunOn="82"><Segment range="85-606" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="AGVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRLCERILATG"/></Q></Feature></Features>
  =<?xml version="1.0"?><Primers nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="KROL358  T7_amp_F" sequence="CTAGTGGTGCTAGCCAAAAAACCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-11-12T18:52:01Z"><BindingSite location="1-24" boundStrand="0" annealedBases="CTAGTGGTGCTAGCCAAAAAACCC" meltingTemperature="60"><Component hybridizedRange="1-24" bases="CTAGTGGTGCTAGCCAAAAAACCC"/></BindingSite><BindingSite location="829-838" boundStrand="1" annealedBases="CAAAAAACCC" meltingTemperature="29"><Component bases="CTAGTGGTGCTAGC"/><Component hybridizedRange="829-838" bases="CAAAAAACCC"/></BindingSite><BindingSite simplified="1" location="1-24" boundStrand="0" annealedBases="CTAGTGGTGCTAGCCAAAAAACCC" meltingTemperature="60"><Component hybridizedRange="1-24" bases="CTAGTGGTGCTAGCCAAAAAACCC"/></BindingSite><BindingSite simplified="1" location="829-838" boundStrand="1" annealedBases="CAAAAAACCC" meltingTemperature="29"><Component bases="CTAGTGGTGCTAGC"/><Component hybridizedRange="829-838" bases="CAAAAAACCC"/></BindingSite></Primer><Primer recentID="1" name="KROL363  T7_seq_1" sequence="GTTATTGTCTCATGAGCGGATACATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-11-12T19:32:18Z"><BindingSite location="2715-2740" boundStrand="0" annealedBases="GTTATTGTCTCATGAGCGGATACATA" meltingTemperature="56"><Component hybridizedRange="2715-2740" bases="GTTATTGTCTCATGAGCGGATACATA"/></BindingSite><BindingSite simplified="1" location="2715-2740" boundStrand="0" annealedBases="GTTATTGTCTCATGAGCGGATACATA" meltingTemperature="56"><Component hybridizedRange="2715-2740" bases="GTTATTGTCTCATGAGCGGATACATA"/></BindingSite></Primer><Primer recentID="2" name="KROL363 T7_seq_1" sequence="GTTATTGTCTCATGAGCGGATACATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2022-06-08T16:23:22Z"><BindingSite location="2715-2740" boundStrand="0" annealedBases="GTTATTGTCTCATGAGCGGATACATA" meltingTemperature="56"><Component hybridizedRange="2715-2740" bases="GTTATTGTCTCATGAGCGGATACATA"/></BindingSite><BindingSite simplified="1" location="2715-2740" boundStrand="0" annealedBases="GTTATTGTCTCATGAGCGGATACATA" meltingTemperature="56"><Component hybridizedRange="2715-2740" bases="GTTATTGTCTCATGAGCGGATACATA"/></BindingSite></Primer><Primer recentID="3" name="KROL628 NLucF" sequence="atatGTTAACCGGTCGCCAGGATACGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2023-03-24T22:07:18Z"><BindingSite location="79-102" boundStrand="0" annealedBases="tGTTAACCGGTCGCCAGGATACGT" meltingTemperature="65"><Component bases="ata"/><Component hybridizedRange="79-102" bases="tGTTAACCGGTCGCCAGGATACGT"/></BindingSite><BindingSite simplified="1" location="79-102" boundStrand="0" annealedBases="tGTTAACCGGTCGCCAGGATACGT" meltingTemperature="65"><Component bases="ata"/><Component hybridizedRange="79-102" bases="tGTTAACCGGTCGCCAGGATACGT"/></BindingSite></Primer><Primer recentID="4" name="KROL350 tul4_6aa_NotI" sequence="atatGCGGCCGCcttaattatttttttcatatagatactcct" dateAdded="2023-03-27T22:32:42Z"><BindingSite location="607-644" boundStrand="0" annealedBases="GCGGCCGCcttaattatttttttcatatagatactcct" meltingTemperature="63"><Component bases="atat"/><Component hybridizedRange="607-644" bases="GCGGCCGCcttaattatttttttcatatagatactcct"/></BindingSite><BindingSite simplified="1" location="607-644" boundStrand="0" annealedBases="GCGGCCGCcttaattatttttttcatatagatactcct" meltingTemperature="63"><Component bases="atat"/><Component hybridizedRange="607-644" bases="GCGGCCGCcttaattatttttttcatatagatactcct"/></BindingSite></Primer><Primer recentID="5" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2023-06-02T19:50:02Z"><BindingSite location="917-937" boundStrand="1" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="917-937" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="917-937" boundStrand="1" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="917-937" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="6" name="KROL672 pdpA_NotI_R" sequence="ATATGCGGCCGCTGCGGGTGTTTTCACCCTGGAAGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2023-06-30T14:52:23Z"><BindingSite location="583-614" boundStrand="1" annealedBases="GCGGCCGCTGCGGGTGTTTTCACCCTGGAAGA" meltingTemperature="73"><Component bases="ATAT"/><Component hybridizedRange="583-614" bases="GCGGCCGCTGCGGGTGTTTTCACCCTGGAAGA"/></BindingSite><BindingSite simplified="1" location="583-614" boundStrand="1" annealedBases="GCGGCCGCTGCGGGTGTTTTCACCCTGGAAGA" meltingTemperature="73"><Component bases="ATAT"/><Component hybridizedRange="583-614" bases="GCGGCCGCTGCGGGTGTTTTCACCCTGGAAGA"/></BindingSite></Primer><Primer recentID="7" name="KROL629 NLucR" sequence="atatGCGGCCGCatagatactcctatttttaaatattcacat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2023-03-24T22:10:03Z"><BindingSite location="607-662" boundStrand="0" annealedBases="GCGGCCGCatagatactcctatttttaaatattcacat" meltingTemperature="55"><Component bases="atat"/><Component hybridizedRange="607-614" bases="GCGGCCGC"/><Component hybridizedRange="633-662" bases="atagatactcctatttttaaatattcacat"/></BindingSite><BindingSite simplified="1" location="607-662" boundStrand="0" annealedBases="GCGGCCGCatagatactcctatttttaaatattcacat" meltingTemperature="55"><Component bases="atat"/><Component hybridizedRange="607-614" bases="GCGGCCGC"/><Component hybridizedRange="633-662" bases="atagatactcctatttttaaatattcacat"/></BindingSite></Primer><Primer recentID="8" name="KROL674 pdpA_perfectSD_F" sequence="atatagcggccgcatcttttactgctatcattgttgacctccttagaacataactcctatagtgagtc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2023-08-02T16:51:52Z"><BindingSite location="606-681" boundStrand="0" annealedBases="agcggccgccctatagtgagtc" meltingTemperature="44"><Component bases="atat"/><Component hybridizedRange="606-614" bases="agcggccgc"/><Component bases="atcttttactgctatcattgttgacctccttagaacataact"/><Component hybridizedRange="669-681" bases="cctatagtgagtc"/></BindingSite><BindingSite simplified="1" location="606-681" boundStrand="0" annealedBases="agcggccgccctatagtgagtc" meltingTemperature="44"><Component bases="atat"/><Component hybridizedRange="606-614" bases="agcggccgc"/><Component bases="atcttttactgctatcattgttgacctccttagaacataact"/><Component hybridizedRange="669-681" bases="cctatagtgagtc"/></BindingSite></Primer></Primers>
  j<Notes>
<UUID>4591ebb6-9a39-4217-bcab-7c3c2315b7e0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:3:0">2023.12.19</Created>
<LastModified UTC="18:51:36">2024.10.1</LastModified>
<CreatedBy>SnapGene License</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         NotI and XmaI                                                                                                d     CHO                E              Y? P                                                                                                                                                             3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   <?xml version="1.0"?><CustomEnzymeSets><CustomEnzymeSet type="1" name="NotI and XmaI" enzymeNames="NotI XmaI"/></CustomEnzymeSets>
