	   SnapGene      AGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGGTACcttgtatggattagtcgagctaaaaagctcatattttttatattcaaactatatacccttcaagctttgaaaaataaacttaattattatatatgttatttagctagtttttttaattaaagttaaaatcgagagcttgtttgacaaaaaaacaaaaaaatttcttgaaaattttttttttgactcaatatctagacttgcaagagcttggaactttgagattgttctaagatgcatacaaattcaaaatgcttaaacaaaaataatttaacaaaggagtaagattgttatgaacattcgtccattacaagatagagtattagttcgtcgtgcaggtacCaTATGatgttcgatcaattaggcaatagtggagcatttcttataaatgctatagttgcagttgttattttagttgttggcttttgggcatctaagtatataaagtctattgtgtatagcctgttgaaagagtatgacaagacagtatcgcaatttttagctagtctagtttacacaatttttttagtgttagtggttgtaattgctttagcaaagttgggggtgccgatatctcctgtgacaggagttctaacaggtgtggtttttggtatctcaatgtcattaaagacatcttataatactgttgcctgtggtattatgctggcatttagtaagccttttgaggttggggaaaaggttgattttggcggaatcaaaggtactgttaaatcaattggttttttgtatactaagcttgatgatgaggatggtaatgaaattgtaatctcaaacaaccttgttctatctaaggtaattactagatttactcaaacagataaataaGGATCCACTAGCTCGTTTCAAATTACCGATGATATCGGACCGTTCCAACTTACCGACCAGTTCGGCAGGTATGTATTTGCGTGCATTCCTATCCAAAAAAACATCAAGCCAAAAGCTTGAAAAAACTTACAACACAGCTCAACAGAGCTAGATTGTAAAACCCTGCTTTGTTAAGCAGAACGCAAAAATTGAATGACTTATAGTCATATCGCTTCGACCCTCGTAGATTAGTAGCCTTGAGCTATTAACTGGTTGAAACACTTACCAAATAAAGATTAAAAGCGATAAAAATGAAAGATAAAGCAGCTAAAAACAGAGATTTTAGAAAGACTATTTTATCAGTGTTACAACGCAATAAAGATGGCTCTTTTGCTACGCAAGCAAATAGAAAGTCTATTCTGTTGCAGGCAACTAAAGACCTTAAAAAGGTAGGGTTTAGCAAGGTTACAGCCGAAAACTTCGGTAATAAGCATTGCTATGCACTTAGAGACCATTGGAGAGCCCAAGGATTAGCTACAGCAACGATAAAAAATCGTTTAGCTTGTCTAAGGTGGTTAGGCGAGAAAATGGGCAAAGAACTACCCGATAATCGAAAATTAGAGATTGAGAACAGGAAGTATAGCGATAATTCAATCAATAAAGCCCAAGAAATCGATTTTAAGGCGATTTCTGCCTTAACTGATAGGCAAGCCCTAGCAATACAATTACAGCGCGAATTTGGGCTTCGTAGAGAAGAAAGTTTGAAGTTTCAGCCTAGTTATGCAATCAAAGAGCATAAAATCGAGCTTAAAAGCTCTTGGACAAAGGGTGGAAGACCACGAGAAATCCCAATTTTGAATGAAAAACAGAGAGAATTGTTAGAAAAAGTAAAAGAGGTAGCAGGTAAAGGCTCTCTAATTGAGAGCGAAAAGTCTTATAAGCAAGCAATGGAACATTTCACGACTCGCTGTCAAAGAGCAGGGATTAAGAATGTTCATGGCTTTAGACATGCGTATGCTCAAGATAGATATAGGCAATTAACAGGGCGTGAGTGTCCTAAAAATGGTGGATTAACATCTAAGCAGCTAACACCTGAGCAAAAGCAACAAGACTATGAAGCTAGAATGACTATTAGTGAAGAGTTAGGTCATGGTAGAGAAGATGTAACAGTCAACTACTTAGGCAGATAAAAAGCAATATAGCTATAGAAGAAAAGAAAGCTATTTTACATAGTAGATCGACTCTTCTTAGGGATTTTATATTTTTTGATAAATCATCTATTTTGCTAGTTAAATCATCAAATTTATCATCTTGTTGTTTGACTAAATCTAAGAATCTATTCTCTTTTTTAAAATCGTTCATGCAAACCGCCTATAGCTTTCTTCTTTTTCTGAAATTATTTGTCTTCACACCATAATTAAATTCCCATTTTTATAAGTAAAGTCTTTTAAAAGCTTGTCAGTCTCTTCTCTAGAAATGTACCAAATTTTACCTATTTTAGGATACTTTTCATGAAGCTCTTCTATTTTTCCCCAGTCCTTTAATAGTCTACCTTTAGAGTCTCGTAAATAGTTATCTTTGTGACAGGGGCCTCTTTTATCTTTTTTAATGTAACTATATGTTATTCCAACGTCACTATTACTATTATCCAAATCTTTTTTAGCATGCCAGTAAGAACTTTCATAACTTAACTCTATCTTTCGACCTCTTTGATATACAACAATAAAGCTATAGCCAGTAGTAACAACCTGTTTTACTTTTGTTAAATCTATTAACTTCTTATTTATTTTTTTATGTTTTTTTGAAAATTTAAATATTTCTATATTCATTCCTACACTTCCTCAAATCCAAATGGTAGCTTATGATTCTCTTCTGGTTTCTTTTCTAATTTTTTTATATTTGCAATAAAAACTCTTTTTCTATCTTTGATTTTTTTATTGTCCCAATTCCTCCAAGTATCATCACAAACCCTTTCAATATCATGTAAATGATGATGTCTAAATATTGATCTGACATAATACAGATCTAGGTCTAGTTCATCACTTAACACAACTTCTCTAAGTCTTTCAGATGCTTCGATTGGTATGTAATCCTCTTTATTTTTAGTATCTAAAAGCTTTTGCTTAAATTCTTCTTCTGTCTCTGCTACCTTACTAACTGTAAACTTGATATTTGTAATCTTACGACCATGTTTTCTGTGATGATCCTTGTCATCATAGGTTACAAAAATATCCGATAATTGATTAATCTCTTCTAGTGCTGGTAATAGGAACTTATTTTTAAAATTTGAATATCTGTTGCTGTAACTTTTAGGTAAATCAAAATCATTAATCATATCATCGACATACAATACGCAATCAACTATATTAGCATACCCTGCTTGTTCGCCTAATTTGCTTTTGAGAAGTAAGTATAATCTGCTTGAATACTTACTTTTAAATGAAAATAGTAACTGTCTTTCTGCTTTAGTAAAGTACTCTTGTAGTTGTATCATGTGTGGCATTAATGACCAATGAAACTCGCAAATTAAAGCACTGCTTTTAGGGTCTGCTTCAATATATGCAAACCAGTTAGCTATCTTCGTTTGTTCTTTATTCAGCCATACTGGCTTAGACATTATTGAGTGCATTAATTGCTTCAATCTCACTCTGTTATGCTTAACCCCTGTAGCTTTTTCAAGATCAGATAGGCTTATCTTATACCTGTGAAACTCTTTATCTTCTCTTTTAACCATTGAGGCAACTAAGAATATTAAGTTTTGTTCTTCTTTTGTAAGGCTATACTTTCCTGCAACAAGAGTATTAGACATAGCTATTTCTTTGCCAGCATTTACATTTTTAACTTCTTTCATAGAACTAGAGTCATTATCTCGATATACAAATTCTATAAAACTTCTATTAGTAAAACAACTACTTCATAAAAAAAAGTAGTTTTAACGATACAAAAAGTAGTTTTAAATTCAAAAAGTGATACAAAAAGTAGTTTTAAATTCAAAAAGTGATACAAAAAGTAGTTTTAAATTTTTTAAAAAAGTGCTTCAAAGCCTTATGTAGCAATACTTACAGAGGATTAAAAAAAAATCTGACAATATATAAAGAGAATATATAAAGAGAATATCTTAGGGGATTTTAAAAAAATCCCACAGACTCAAAGACTTTTTTGACTTTTTAAATCCTAGAAACTATACTTTAAGTACTTATTTAAGTACATGGATTTAGATTATGCAAACCGTTAATTATTCAACTTTTAGAAATGAACTATCTGATTCAATGGATAGAGTAACAAAAAATCATAGTCCTATGATTGTAACTAGAGGTTCAAAAAAAGAAGCAGTTGTTATGATGTCGTTAGAGGATTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATTCAGGGCGCAAGGGCTGCTAAAGGAAGCGGAACACGTAGAAAGCCAGTCCGCAGAAACGGTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGTAGGGAGGATTTTATTATGTCTACTACTTTGGATGATACTGCTTATAGATACAGAACTTCTGTTCCAGGTGATGCTGAAGCTATTGAAGCTTTGGATGGTTCTTTCACTACCGATACTGTTTTTAGAGTTACTGCTACTGGTGATGGTTTCACTTTGAGAGAAGTTCCAGTTGATCCACCATTGACTAAGGTTTTCCCAGATGATGAATCCGATGATGAATCCGATGCTGGTGAAGATGGTGATCCAGATTCTAGAACTTTCGTTGCTTATGGTGATGATGGTGATTTGGCTGGTTTCGTTGTTGTTTCTTATTCTGGTTGGAACAGAAGATTGACTGTTGAAGATATTGAAGTTGCTCCAGAACATAGAGGTCATGGTGTTGGTAGAGCTTTGATGGGTTTGGCTACTGAATTCGCCAGAGAAAGAGGTGCTGGTCATTTGTGGTTGGAAGTTACCAATGTTAATGCTCCAGCTATTCATGCTTATAGAAGAATGGGTTTCACTTTGTGTGGTTTGGATACTGCTTTATACGATGGTACTGCTTCCGATGGTGAACAAGCTTTGTATATGTCTATGCCATGTCCATAATgcatgCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAG  -V  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                                                                         	      	                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         	                                                                                                                                        
                                                                                                                                                                                                                                                                                                     
      !                                                   
      	      
   	                                                                                                                 	   7                     x      	      B   Q   6         D   n                                                                                                                                                                                                  	  	                                                                                                                                                                                                                                                                             	                                                      
                                                                                                               
  !                 
  	  
 	                                       	 7       x  	  B Q 6   D n   =  =  >	  >+  A9  E  L|' 1I	.Ncl~^	
o ;P{V kk1	 W  wk|PkW,Xw#_LJ	frLJ	TfrLJ	frLL{u.o]^d9m
b_\
 nol	G4
:? B8  B$nfJ 5# 	3N=			7wh8lnf{Y{Y- h @mk-{Y?1~o~nfi	

@*%
x@	
%  nf  B$?\
nfJNur*)##% 5\2*`P	ciT=	B1~-{Y
5		
u6 S 
/`	!TeP/_
?W %qm		:a]}K*]lr*8G 1	k4
{qYF t$	
]x=i`$j
[`P4 gJ?4 g*##)>Q|58
}fe0w	?]@K.6O;#3!v  y	A
%3 mc?v,>c]
`ja*[L=c`3 mc>?v,-(XwM3 mc?J52v,>h ja yN?G~h <K 6U@*'Hsw&


0E@Wv+&nBseJads!`_n	Z	3ZWX&BX &rQ. S ( 	^	?
qTrClxA!9/a	<2Do{'hu$,K=xqekN4V
t


(bCa|/in				
v{~;F
?
T	r!4V

C( ^	qClxAJ52hX &rQa
jv-* w	

JH{CU  "g  	K6cCS  E^2~LALh
 C	95 u   2YyH.o>YNVe		

jl6#qr :h"v\ef  ^ o_g;!<[%F0/		Q<ZS@ Kq= ^$ ^
^
4Oj| a]UkQb[3 y	

1}']$ 5 u     2YyH'.o2>Y-NVe		
^


jl6OX#cq^r +:h
#>W"1v4OSx\eHafPj|     %'KMOGIpym5D.	y				
y
{




*FnMFQdl&(R Jcn:<GI3H1L&}'o]n{(xz|T
Z62 >7{	*j$|p2Z  1zwm&



q00a]fUkEc?tQAP@$bI[3d `y7 @ L    @8D7;Z
zVaA]jv +Ubk(N>QUjYIbt [3Ezg   EfW S	|5>					8xpia9kj `

YUsN""@H{. i Cdhna3RQ7X3	n			.
o

;%uAn?'bhylElW5 A u    2YyAEH.o>YcNVel			
8




jl16#Zqr :h|["Iv6\Iezfu!tQK
EM_G1  &               Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пfy9IIMJW\UN	5FCM%O]TTdsDZDQ6<ٞXYBVPoy;*է|^JBbj26oUWmOU:W]ӵ9%_㨥4\Z-:$i$^
ޟVTiTUaQ4WDjQ橴ueFsSVEKyQUB@5
tW%95U[*N 1`5EZDhERdZTElj1MgZtZ$E*Oe[ST}VjWDEU%Q	4%CLU.MM`]q@iE:ɎdfCZ]!"UnSN]MTQXy	F%מѕSYU9vI[njJVVۢEVNiZVjRTSXVAaN뚫/d.Y)tXk-i[^n~u9Iuz릦*jVjkkzV`*jfYU/־&uY%a.VhntnB䭷zRюnU:k~^%zتod)YeHAzjZ8BVmms[^fgj:n:zzaVIxIR{J&؈6XWV7fcWғx:Gi#ih[:_FC韈WהY*%g]DGd殣jv^EKiZUIW^zUTqU$VTuTZRRJjUHTbMgDUUnfeDYQnU[GGo^gII5|X^nDU[da^D+UTSJ),Aܬ-Nl @:7W` =dp(+dx]je<j悕VZ1jU5Tw$T\#XuW53TEM	:410`tZ=8?%
OT`nQl6QsB΀uÎ<4?
|~/	.yFt^BNhS+y*Jx*ޡJ*F[[8!FoF(`(]hxFhR]`	`*xZDTB8-J}b;ӚEzgqx]Nf.cؽ6?1CLÛ 4;P91Hoߣ<EI0Ío~?k}LWlr{<.AqD@;3C3cn!f UΫ A2fNX:#l3lϮhUADIv"jTF8Q#q )]0o?*aީR &hsM{<kXz
{}-TۧIDZcH}]^uu m{>|=WL}>$u3W^^z{]idx(}?
8en	7=%?&)p5ѳd#6^-RfzWPnBo[UJxLF2[:5uU}L mD׺	x:Dg$JCz^Mg9o} H1.G)e7t:|^2EL:PPЛSt3q: W>	/;- W=5>p;v+) ei|O4Ĵ     F  H     )  )       !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  G<?xml version="1.0"?><Features nextValidID="20"><Feature recentID="19" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="5038-5660" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF_nat+MglA-VSVG"/></Q></Feature><Feature recentID="18" name="nat resistance" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5055-5627" color="#a6acb3" type="standard"/><Q name="note"><V text="nat resistance"/></Q></Feature><Feature recentID="17" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8090" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature><Feature recentID="16" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4717-4766" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="15" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="811-843" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="14" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="972-994" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature><Feature recentID="13" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL2"/></Q></Feature><Feature recentID="12" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6290-7147" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="11" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4855-6269" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="10" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2660-3676" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="9" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2235-2654" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="8" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-2012" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="7" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="6" name="cold shock protein" directionality="1" translationMW="131.18" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="67" hitsStopCodon="1"><Segment range="601-607" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="601-607" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="30S ribosomal protein S21" directionality="1" translationMW="7847.44" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="846" maxFusedRunOn="846"><Segment range="607-801" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q></Feature><Feature recentID="3" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="607-801" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="acetyltransferase" directionality="2" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="173" maxRunOn="783" maxFusedRunOn="783"><Segment range="801-801" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="801-801" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-801" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL38 OE_rpsU1R" sequence="atatGCGGCCGCaataaatgctcttctctgagaaattctt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="773-812" boundStrand="1" annealedBases="atatGCGGCCGCaataaatgctcttctctgagaaattctt" meltingTemperature="67"><Component hybridizedRange="773-812" bases="atatGCGGCCGCaataaatgctcttctctgagaaattctt"/></BindingSite><BindingSite simplified="1" location="773-812" boundStrand="1" annealedBases="atatGCGGCCGCaataaatgctcttctctgagaaattctt" meltingTemperature="67"><Component hybridizedRange="773-812" bases="atatGCGGCCGCaataaatgctcttctctgagaaattctt"/></BindingSite></Primer><Primer recentID="1" name="KROL37 OE_rpsU1F" sequence="atatGAATTCaggaggactataatgctaagtattagagtagatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="584-627" boundStrand="0" annealedBases="atatGAATTCaggaggactataatgctaagtattagagtagatg" meltingTemperature="61"><Component hybridizedRange="584-627" bases="atatGAATTCaggaggactataatgctaagtattagagtagatg"/></BindingSite><BindingSite simplified="1" location="584-627" boundStrand="0" annealedBases="atatGAATTCaggaggactataatgctaagtattagagtagatg" meltingTemperature="61"><Component hybridizedRange="584-627" bases="atatGAATTCaggaggactataatgctaagtattagagtagatg"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>fb7696a3-e0ee-4c78-abd1-912d39f9a234</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="22:14:46">2019.2.12</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  #     u  +        +Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пfy9IIMJW\UN	5FCM%O]TTdsDZDQ6<ٞXYBVPoy;*է|^JBbj26oUWmOU:W]ӵ9%_㨥4\Z-:$i$^
ޟVTiTUaQ4WDjQ橴ueFsSVEKyQUB@5
tW%95U[*N 1`5EZDhERdZTElj1MgZtZ$E*Oe[ST}VjWDEU%Q	4%CLU.MM`]q@iE:ɎdfCZ]!"UnSN]MTQXy	F%מѕSYU9vI[njJVVۢEVNiZVjRTSXVAaN뚫/d.Y)tXk-i[^n~u9Iuz릦*jVjkkzV`*jfYU/־&uY%a.VhntnB䭷zRюnU:k~^%zتod)YeHAzjZ8BVmms[^fgj:n:zzaVIxIR{J&؈6XWV7fcWғx:Gi#ih[:_FC韈WהY*%g]DGd殣jv^EKiZUIW^zUTqU$VTuTZRRJjUHTbMgDUUnfeDYQnU[GGo^gII5|X^nDU[da^D+UTSJ),Aܬ-Nl @:7W` =dp(+dx]je<j悕VZ1jU5Tw$T\#XuW53TEM	:410`tZ=8?%
OT`nQl6QsB΀uÎ<4?
|~/08=+8(0hR<ARom50g:ßS6ܞOaQ6x;Pp`>:<Y`klxÀ0|vLξp<ƳL>ĮZPQ&k]ȳk0ڣ;Es%Qm(H
vnL%DEʯXa7T93	5^6wKtU6aQ8_a!xקqAA9|v b^ϡ_aOg!q5]r旃ZY0eJ4z;Ã[<!2mMF	OɲJnw-ydtlxiMGԩ홞hjUjTꡖor+-EQAF̖㎍]U_|**<Q9B^3F}Y<޵|;[H8e,{QJ|C4p ?N1qgLS=οT0t&@]L@N ςnK=2ƪ!@UMs+݊ok||;<1-!10     F  H     )  )  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  N<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7467" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature><Feature recentID="16" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4717-4766" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="15" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="811-843" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="14" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="972-994" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature><Feature recentID="13" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL2"/></Q></Feature><Feature recentID="12" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5667-6524" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="11" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4855-5646" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="10" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2660-3676" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="9" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2235-2654" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="8" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-2012" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="7" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="6" name="cold shock protein" directionality="1" translationMW="131.18" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="67" hitsStopCodon="1"><Segment range="601-607" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="601-607" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="30S ribosomal protein S21" directionality="1" translationMW="7847.44" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="846" maxFusedRunOn="846"><Segment range="607-801" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q></Feature><Feature recentID="3" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="607-801" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="acetyltransferase" directionality="2" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="173" maxRunOn="783" maxFusedRunOn="783"><Segment range="801-801" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="801-801" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-801" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL38 OE_rpsU1R" sequence="atatGCGGCCGCaataaatgctcttctctgagaaattctt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="773-812" boundStrand="1" annealedBases="atatGCGGCCGCaataaatgctcttctctgagaaattctt" meltingTemperature="67"><Component hybridizedRange="773-812" bases="atatGCGGCCGCaataaatgctcttctctgagaaattctt"/></BindingSite><BindingSite simplified="1" location="773-812" boundStrand="1" annealedBases="atatGCGGCCGCaataaatgctcttctctgagaaattctt" meltingTemperature="67"><Component hybridizedRange="773-812" bases="atatGCGGCCGCaataaatgctcttctctgagaaattctt"/></BindingSite></Primer><Primer recentID="1" name="KROL37 OE_rpsU1F" sequence="atatGAATTCaggaggactataatgctaagtattagagtagatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="584-627" boundStrand="0" annealedBases="atatGAATTCaggaggactataatgctaagtattagagtagatg" meltingTemperature="61"><Component hybridizedRange="584-627" bases="atatGAATTCaggaggactataatgctaagtattagagtagatg"/></BindingSite><BindingSite simplified="1" location="584-627" boundStrand="0" annealedBases="atatGAATTCaggaggactataatgctaagtattagagtagatg" meltingTemperature="61"><Component hybridizedRange="584-627" bases="atatGAATTCaggaggactataatgctaagtattagagtagatg"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>fb7696a3-e0ee-4c78-abd1-912d39f9a234</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="22:13:58">2019.2.12</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       t  %        %Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&ПfcD_jEzTSQ:Dd4rTUEFMW=N)TMEEjh͙%eZ|a4&&#kUvSUzu;]U:SEUaٳFO9]eFLeVSNUtFjH\T`55dT(וT$
POzYMޑh?Pi9\eUB
CVT^lUF]&LemeOVƩC^OuLŨDQRJ)mQ6U:U;u@GEo֨XUtDZ)RUBT;hDRڷ-Q4XFFd52/X iv4߄MuaNEHQ)`b]-yU59EQSd@jmjm(y[[l_>d몸Veke%KU:-.jK앒@>ꛪyriպMIEz^YS=$_)[^jjbVffwoV	:a낮j5^nkRmWbVbfgF.Jz]׮.	o&S:V닸Wn}!-BUZ[	dUYz%mV[fg5i6ϖIIgVd'$mia{5iSx.:f5})9ʊkOgv6{c:Qd:n{}yERPz5IvNj=6jd^Dծe^u筕UI*RJkUHgUI*U!U$VTO$ztAUVfTEf jUt{TzVzcWZk^`dIuUK%BUE4bD=V
#~Uz@
ܒ?G
?r/rHWuꆦPƬ>h)Ua8j]UsuZ3P8ALrEOK8NxnPsS5DTГH9
ML_Ӏ3P5I^=A` mFaʳau74+<ka7]l8ȯ-3@3-Gaxq3lp2ӈrxS@x8#4-~&8cX6s9}1]lLM,mm OC͏A,cś`V:ǈ<8md!8Q:1`8|쎧	k4ͳ>J녡Ua&ڼ?>*W8!RUFҀĀl^tQJ\zJ㘃0oɡQ5,gya(IESn6%i#ew=zzΪ`%(N}\q2X5(^ix1yNu`CTA388*hᕸ .&4 `$qט\VGF	x}JٙF^ZAQn}V!*2$UTon8U}0^%M4g՞+H)}Q{7ϝ)= RȺ|
d3L, 
xF~tNa3@ECBo	L4D\&d,
߸z,j 0O\0¾8>خɧϨHN<\|LS      F  H    #  #  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  _<?xml version="1.0"?><Features recycledIDs="9" nextValidID="19"><Feature recentID="8" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="595-795" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="7" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="795-795" color="#00e600" type="standard"/></Feature><Feature recentID="6" name="acetyltransferase" directionality="2" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="173" maxRunOn="777" maxFusedRunOn="777"><Segment range="795-795" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="601-795" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="30S ribosomal protein S21" directionality="1" translationMW="7847.44" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="840" maxFusedRunOn="840"><Segment range="601-795" color="#ff0000" type="standard" translated="1"/><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="595-601" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="cold shock protein" directionality="1" translationMW="131.18" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="67" hitsStopCodon="1"><Segment range="595-601" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1131-2006" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2229-2648" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2654-3670" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4849-5640" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5661-6518" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="966-988" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="805-837" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4711-4760" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7461" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:51:57">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       A  \        \Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пf6ghVFNʵil+קҰИUSNUttNFW)M5VKfαI>旂wՕU1XTeM9UTym"sYQՑR渣C^T~P*MB<U	e6czD}DAMqVʓ)EXQyeVt1?Q[jxd5?Y֝3:EJ*5DVTqZaUhUITBM	PKSSXj{8DQbv2c֗@HU`۔ӗ~U5y9i!D-DQt4eTdEENjFǺfVۚթ袡nQnkU5|Zj.}ک.T贻V'ժ~jgSKY.~KVJn n%byoV6=%e{g]eNd]~*niz驪Z޾սX$醻
ZU{I_mXae+mw^ch&%jNZ.㟧^j%q*
uVohin8%^Vf%ꐕ[imlvuי:+>ZE%&[XU^.:T蒉"եM@Y*+=hHZ:)$Fѐg뺥5iJCQ&9ڝyngVzUמUU$\gUI*U!U$VTuTZUR:>YU[YQfTEUVQz[WRzjRvi_)izV%V/h
UҊa)k+<:S[ ;+ƤUi+rHl*Y {^{_.k@jUuVZk@@0]=/4W-99@ULSBN :vdx*51|N 	BS%x:<4GA*͆`hЮ D99tuc"8 º?A49q3N#-L n;T`c8Άvͱ17'`S豴nM=<^+=36?11mX< "9(9hD0ŀ:&;6Ϯlq+TIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLAL     F  H  Q  Z  Z  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  A<?xml version="1.0"?><Features recycledIDs="2,3,4,5,6,7,8,9" nextValidID="19"><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="930-1805" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2028-2447" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2453-3469" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4648-5439" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5460-6317" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="765-787" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="604-636" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4510-4559" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7260" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:51:53">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                   Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&ПfP)iYn!CNUD
[!3Wd[a,Fh&=eWREfYTYg9QiPiyOjD䓑iS,n	TXQZFF*;pkUQ)3NfuX$TwNVf]E'f6ZTUjTRZP͙%eZ|a4&&#kUvSUzu;]U:SEUaٳFO9]eFLeVSNUtFjH\T`55dT(וT$
POzYMޑh?Pi9\eUB
CVT^lUF]&LemeOVƩC^OuLŨDQRJ)mQ6U:U;u@GEo֨XUtDZ)RUBT;hDRڷ-Q4XFFd52/X iv4߄MuaNEHQ)`b]-yU59EQSd@jmjm(y[[l_>d몸Veke%KU:-.jK앒@>ꛪyriպMIEz^YS=$_)[^jjbVffwoV	:a낮j5^nkRmWbVbfgF.Jz]׮.	o&S:V닸Wn}!-BUZ[	dUYz%mV[fg5i6ϖIIgVd'$mia{5iSx.:f5})9ʊkOgv6{c:Qd:n{}yERPz5IvNj=6jd^Dծe^u筕UI*RJkUHgUI*U!U$VTO$ztAUVfTEf jUt{TzVzcWZk^`dIuUK%BUE4bD=V
#~Uz@
ܒ?G
?r/rHWuꆦPƬ>h)Ua8j]UsuZ3P8ALrEOK8NxnPsS5DTГH9
ML_Ӏ3P5I^=A` mFaʳau74+<ka7]l8ȯ-3@3-Gaxq3lp2ӈrxS@x8#4-~&8cX6s9}1]lLM,mm OC͏A,cś`V:ǈ<8md!8Q:1`8|쎧	k4ͳ>J녡Ua&ڼ?>*W8!RUFҀĀl^tQJ\zJ㘃0oɡQ5,gya(IESn6%i#ew=zzΪ`%(N}\q2X5(^ix1yNu`CTA388*hᕸ .&4 `$qט\VGF	x}JٙF^ZAQn}V!*2$UTon8U}0^%M4g՞+H)}Q{7ϝ)= RȺ|
d3L, 
xF~tNa3@ECBo	L4D\&d,
߸z,j 0O\0¾8>خɧϨHN<\|LS      F  H        !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="19"><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1635-2510" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2733-3152" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3158-4174" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5353-6144" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6165-7022" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1470-1492" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1309-1341" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="9" name="P018 FTL_0552fragF" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="773-799" color="#a020f0" type="standard"/><Q name="note"><V text="P018 FTL_0552fragF"/></Q></Feature><Feature recentID="8" name="P044 INTpEX_FTL0552" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="686-713" color="#a020f0" type="standard"/><Q name="note"><V text="P044 INTpEX_FTL0552"/></Q></Feature><Feature recentID="7" name="P238 FTL_0552F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="616-642" color="#a020f0" type="standard"/><Q name="note"><V text="P238 FTL_0552F"/></Q></Feature><Feature recentID="6" name="P301 PmrA_comp_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="600-627" color="#a020f0" type="standard"/><Q name="note"><V text="P301 PmrA_comp_F"/></Q></Feature><Feature recentID="5" name="P017 FTL_0552fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1272-1299" color="#a020f0" type="standard"/><Q name="note"><V text="P017 FTL_0552fragR"/></Q></Feature><Feature recentID="4" name="FTL_0552" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="616-1299" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0552"/></Q><Q name="db_xref"><V text="3952730" predef="GeneID"/></Q></Feature><Feature recentID="3" name="FTL_0552" directionality="1" translationMW="25520.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1344" maxFusedRunOn="1344"><Segment range="616-1299" color="#993366" type="standard" translated="1"/><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="product"><V text="two-component response regulator"/></Q><Q name="note"><V text="/dnas_title=two-component response regulator"/></Q><Q name="locus_tag"><V text="FTL_0552"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="YP_513318.1"/></Q><Q name="db_xref"><V text="89255956" predef="GI"/><V text="3952730" predef="GeneID"/></Q></Feature><Feature recentID="2" name="P229 PmrADel5R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="600-624" color="#a020f0" type="standard"/><Q name="note"><V text="P229 PmrADel5R"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5215-5264" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7965" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:48:21">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                   Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пfy9IIMJW\UN	5FCM%O]TTdsDZDQ6<ٞXYBVPoy;*է|^JBbj26oUWmOU:W]ӵ9%_㨥4\Z-:$i$^
ޟVTiTUaQ4WDjQ橴ueFsSVEKyQUB@5
tW%95U[*N 1`5EZDhERdZTElj1MgZtZ$E*Oe[ST}VjWDEU%Q	4%CLU.MM`]q@iE:ɎdfCZ]!"UnSN]MTQXy	F%מѕSYU9vI[njJVVۢEVNiZVjRTSXVAaN뚫/d.Y)tXk-i[^n~u9Iuz릦*jVjkkzV`*jfYU/־&uY%a.VhntnB䭷zRюnU:k~^%zتod)YeHAzjZ8BVmms[^fgj:n:zzaVIxIR{J&؈6XWV7fcWғx:Gi#ih[:_FC韈WהY*%g]DGd殣jv^EKiZUIW^zUTqU$VTuTZRRJjUHTbMgDUUnfeDYQnU[GGo^gII5|X^nDU[da^D+UTSJ),Aܬ-Nl @:7W` =dp(+dx]je<j悕VZ1jU5Tw$T\#XuW53TEM	:410`tZ=8?%
OT`nQl6QsB΀uÎ<4?
|~/	.yFt^BNhS+y*Jx*ޡJ*F[[8!FoF(`(]hxFhR]`	`*xZDTB8-J}b;ӚEzgqx]Nf.cؽ6?11mX< "9(9hD0ŀ:&;6Ϯlq+TIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLAL     F  H     )  )       ,7zXZ  ִF !   t/ ] 3D].?I*ә_.ZvќP,Vj,ު~mC<ґ?vd;!U''yzYl.vU[Qec <L3cGq~=X?r+<ɵؿĊG]1|=ty?izd@Ppp}ݽ<,c^D2)=0m(w+}[)0|cK7tDi|6?i6J$MEԎ.j7ȡdw}fHAKnm/<`!H|r,7\LD'j ?.|'J&29?J EՋ%1DiFu?\mo]\`[SsGrnڦ"$X=i`hzЉwA<J$"%N1]I11Ҁz_yjs(J:tI
x`Z|rC N4NS!
ibl">,Ѣ?=	GԵZ`k`jǨ9.p*G	X+{	i%Y%NX%/W?S/;WG٨p-oEo44<ڭ]T>nۯl'pӑy.w2uq2kr}w|zl	إڒ߭7=|jPM,	O!<{Rϒ!JTXr4蛲A!xWMz0҄M+HV/|7GK2([	e^1麁u$#pf/qvv0góHy0TOv
J)
@j&Kt|(RT.k#RUCMP·be@FBrS#.]%lYSBǴÙY-%_bA:]X&Fy	̖"i?}&L٠G錞21XT?Mښ5Ч$F41TgwJTR{"Q g2CQ) -6e>NVVR2?طl
dS%Mk
2n;Fs)a098m5`S*w6+"MxHv@,|΅u*bU7GO˨p 4ڝ߶z$>@a3$x*֜`ٖ&BQt	{'vkh$U?Ǳɝ
|5yaͣDqW{Xab}b=XPu.3ΒKūQv3;nV<IސiT1|>'f`AQef51l`ؔ#Q7y)`q@)UwGNiT
[M#읜=U
[$POwMp g^.]u]ʟ# 4=Tv@$eJU1<,~b.2Vfc*f9'l.4;0	(FF~HаJϗxʌOFh!+{pq]A-yӤ_U19u4"J:>\^^Xw"nIK}lV=VP+e򖃵K!GiR@D.G00HO :Yb1:qaЩ0q0|#:d$z    pW>p A  (볔g    YZ                  fjn*V䖟d]Bi֭&uUtkUUV=6YC]&F.hF}U~)-Fl7^5_ywU%yNUhx-CdzO]yV]xי6QXy塱vن          
        47zXZ  ִF !   t/] 3D].?I*ә_.ZvќP,Vj,ު~mC<ґ8hlFOlY.k(DW`!
 	KJ9rEPܤ$FcprMUq3+Xfg=GjLy'Ev-Ðл%O|HYU`{NbL/O
v."3׹)>p!U{ZMs9IA)iyfEO
.na	eVԁK#=>`gX?hf9T>	?1U@̳&07Wiy5d"2ۆ-2 ܪ^x^ͼK]EH8)p.R-N H@i9S|p*cǧ鐤M8H 
)c/Yr]͡Ɵ9կgP0X#>ґl,!U@VW⊎tyqb	?QNH*ꞖG:.\WpLq~RQbsdBYt5Q,Vs ?e~Be60ȍ=r@C%AhA]:%h׍=`jx&wFZKC(<s`/ܝWB>a?21$ [,`քʺܕ/sy)>L1\JU8`Vl{W`)eշ4e,oG?$N/]>+U,H$M-xBXP$⬪~㤂ߩ,d;v2||Xf=%?fɈ+0@]y!H*6!N~#bۛ$t~uOVgvkF=0Gw~d$RKv^͗$Uh7fYGtq<Ia-/΍]t_u^wezg26ke`{kz`} Qcͳz;ߑ6<-kΗ7CPZŻz{
;Q~	ζ܊SAݿHS>v.piƗw?}r}W;	KO(	6ƃܣeC5XQ߇e6;ŅtЗvb|DZ P5xoP  UI 
%  BWg    YZ  	L7zXZ  ִF !   t/O	] ˇf{3(1v%f M*	mj)p80*i	NY]QF7&!nB&xNn}7FvSEE#*s9W[J
ny0wOuYX6V`YJ0N	hQII/ɫ`0z;tmKi4TpкXxY0q?:jth_5@N?1wzO`d1"8 bqtc-˝|K=U6Lϵ)朄9`Sb4v0s4,$㛥cm&L6Uw($ҫSr]?՝&}BڝGN3v1f8	xC3 7ҹݟp?o;2|XX|4%CwV m/0nޗJ4Hy3  iSQCg ,d, ؾVfYZv1c#1y)^'Y.	
GɏL] Q=mܦ(7fe,Gƙ$LT5bG!ݳc*#2:?LA_@pHx4Ԡe,O&?t	4S (W%fɭ|}zeG%w ޗ@v !
/xÆbdgp	d>ǆUXs,!!|)œtf`)[{>sV]p!yn)G/6s$Ue\=}hQ^8-0fF6ƻ^Ԁhi:*Ri)&^L92uۖOUr׮a=ӨUq\ZyQ _z!6R"#S4x%`T![NsSq_RF\:ە>ae[E@gO<:BFΖ&t9[9N&n:nȄ2624Գ]q@uהHۅ`3_Sy?ܭi5b+4*K$xk>/)B?S_H.`]mh("/(b/lxWb+
bhUrӽ	gâ|yb?'=BRrSO 8{	)Bw?Ku PbmWU
 ՚(hx:n +P=*<|utWֺuWv`"ƱcSqv_r;vd8Es7b!ڣ-\(f˓+xHIâF*ma2.!3lBB@VtyLEhv^ݴ1CL.89v=|xvshya܇u^Ûx%2W)Ji87W'yHDx21M*WtG}Ǚ&#RڝC	JeGT!cg8gPJ7fJ.@<ڵB<*|B-zz]4mp;k;NHT6t_-:#^<MV%@#_i0BN#"AСkTµtLY*9<~|t4p!D04_J"l'bM1<)--tԍ1_ms+|#}zv ˔Hm$=0IR_h3cHv̄Rl7l,1
iS2x*u9ed|Ԝ\$iSME/<?jV|p-+`K6nI^o]Lz:h|؆q8#%\/BbLM=D &0(e#e!_s)mqMέf3xSsiEd^X@B+=WERE"	U3m:BV_dQn-W]'6Z\Q;cIZ]DFfU$h`r{=ꕉ_)bZ/<WS|&a6F#  n윱G/Ƅ̜ZB!SjwȢ<@>ཛt.XޘDjgP˄gҠFyaNId=L5qxQqB'oEB0uG"wKxD^[hL+#K{_xQGS   '(ٺU,  y:5g    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  6<?xml version="1.0"?><Features recycledIDs="13,4,11,12" nextValidID="20"><Feature recentID="0" name="nat resistance" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5296-5868" color="#cc99ff" type="standard"/><Q name="note"><V text="nat resistance"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4958-5007" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="2" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1213-1235" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature><Feature recentID="3" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL2"/></Q></Feature><Feature recentID="5" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2901-3917" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="6" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2476-2895" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="7" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1378-2253" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="8" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="9" name="FTL_1753" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="590-1087" color="#00e600" type="standard"/></Feature><Feature recentID="10" name="ion channel protein, fragment" directionality="1" translationMW="17816.11" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="590-1087" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MFDQLGNSGAFLINAIVAVVILVVGFWASKYIKSIVYSLLKEYDKTVSQFLASLVYTIFLVLVVVIALAKLGVPISPVTGVLTGVVFGISMSLKTSYNTVACGIMLAFSKPFEVGEKVDFGGIKGTVKSIGFLYTKLDDEDGNEIVISNNLVLSKVITRFTQTDK*"/></Q></Feature><Feature recentID="14" name="AmpR" directionality="1" translationMW="31557.36" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6221" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="6153-6221" color="#ccffcc" type="standard" translated="1"/><Segment range="6222-7013" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="15" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7184-7772" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="143-148" color="#ffffff" type="standard"/><Segment range="149-166" color="#ffffff" type="standard"/><Segment name="-10" range="167-173" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="107-128" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="181-197" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="205-221" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  {<?xml version="1.0"?><Primers recycledIDs="1" nextValidID="3"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="KROL668 FTL_1753_BamHI_R" sequence="atatGGATCCttatttatctgtttgagtaaatctagtaat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2024-07-01T22:57:48Z"><BindingSite location="1057-1093" boundStrand="1" annealedBases="tGGATCCttatttatctgtttgagtaaatctagtaat" meltingTemperature="58"><Component bases="ata"/><Component hybridizedRange="1057-1093" bases="tGGATCCttatttatctgtttgagtaaatctagtaat"/></BindingSite><BindingSite simplified="1" location="1057-1093" boundStrand="1" annealedBases="tGGATCCttatttatctgtttgagtaaatctagtaat" meltingTemperature="58"><Component bases="ata"/><Component hybridizedRange="1057-1093" bases="tGGATCCttatttatctgtttgagtaaatctagtaat"/></BindingSite></Primer><Primer recentID="2" name="KROL721 FTL_1753_NdeI_F" sequence="atatCATATGatgttcgatcaattaggcaatagtg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2024-07-01T22:57:48Z"><BindingSite location="583-613" boundStrand="0" annealedBases="CATATGatgttcgatcaattaggcaatagtg" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="583-613" bases="CATATGatgttcgatcaattaggcaatagtg"/></BindingSite><BindingSite simplified="1" location="583-613" boundStrand="0" annealedBases="CATATGatgttcgatcaattaggcaatagtg" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="583-613" bases="CATATGatgttcgatcaattaggcaatagtg"/></BindingSite></Primer></Primers>
  f<Notes>
<UUID>e270e690-d768-46b0-acf7-15adf2d66a6b</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="22:58:42">2024.7.1</Created>
<LastModified UTC="23:0:48">2024.7.1</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         6+ Cutters                                                                                                   d     CHO  d              E              Y? P                                                                                                                                                              )<?xml version="1.0"?><CustomEnzymeSets/>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
