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   Wp		0	,
.	p Y g %3cph9	_	
  _wC
j

-^v3  Z!L

\0k l<Dw}QHKA ;q{H

=iL  |0g0Y%% rM	 G_Cc	QfYr [.6b{i?  G~T>:w		
>ZD!ku  JQuA	a

T &
NA	8r`h B ]*d'B  _b(ll		
j F ]  ~	PrH V w   --"I)
>
S
[
C#Xe +R<!	y	1
7
gP G<	
:

6@{6  
B.>k]	
6b   +yVt	-m u2d(Vg


@ B{c	/q  :hq
	1	
 " d    Rn@qp-



qV    (Xa|   +&4ij:	
>+w h [3lq,u
y #x6d+	 
%

3Iv*Ix Z p 5WH`	
\- ]  2AL52k
w

(4 l   C?e

1K@Vw7 8.x.- K  =`xVD

_jn  / 4#
1l  	w+<5"	$		M



]Pj
 E 	5b s [g?;`* S=x
R=GB Cx	
~2N mi~5L-} ]YEnLa
#I  G j  |c

>0{ sY_

I
m } %	<WfrrY	jM %[OZalDu

"!& }18	C				

& N H[:Wz f@Vhu(1LPSz|			"
4
E2  
 ; 	om
 ZLM			` iJ0		
 sNHdj
 	(				R	 vK
s Ij`"c_xt<  HDdggbZ 	/	
'
-  zCvG
K

|,<KGj@ f-/	5h P\9
)7cS _j$ U v   ^a*HQ';} 	k	x		
OEOq" 	tcU\		
 ?zl !     '9g?b	
  *Z"2k 	

H[=*wv \ 17KB1

,Uim  . D r >_v	) hR
"F  	b
F/ ZU'KkOy	B	
!9DV Y_l  	^fsa
  u	,i   )Y!)[j		
 - C ^+E(E  YJtj	
 +Ce  , (	


*CD  )  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      	                                                                                             	                                                                           
                  
                     	                                  	                                              	   	                                                            $      6   1         V   U      
         5                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 	                                     	                           
      
       	            	                	 	                    $  6 1   V U  
   5   X  Yc  Y  Y  \q  _  d
!  ZB		

H_>mK^Q0 5t	t	2
	pc 3
rB	  r*+ko	~			=

pC~Z
3/	q	:#:W?	#4q	r		,0) 	 zk
djD	q	4 5. 5.]d;
EPT
5	n 3_
T u  
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B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    D   I   R   T   ~    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="81-87" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="15-62" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="108-126" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="125-125" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-612" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="163-612" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="163-612" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-253" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="314-334" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-127" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="613-662" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="233-252" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="233-252" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="233-252" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="233-252" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="313-333" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="313-333" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="313-333" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="313-333" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:47:54">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  Uʑ 9Z4b	gdYUIUVZSmlЩ18{);NFxӞxMN8nS׍TY^eYT׉gYeF`E
#gS֞RcQqz^%Ҕ%$RNXQIl놵fZJUU;   ;   @   I   K   u    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-78" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="99-117" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="116-116" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="118-603" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="154-603" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="154-603" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="225-244" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="305-325" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="118-118" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="604-653" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="224-243" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="224-243" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="224-243" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="224-243" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="304-324" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="304-324" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="304-324" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="304-324" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:47:10">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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   <?xml version="1.0"?><Features nextValidID="20"><Feature recentID="0" name="pdpA UTR" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="625-648" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="607-615" color="#a6acb3" type="standard"/></Feature><Feature recentID="2" name="hypothetical protein" directionality="2" translationMW="360.41" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="4" maxRunOn="82" maxFusedRunOn="82"><Segment range="607-615" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="VKD"/></Q></Feature><Feature recentID="3" name="FTL_0126" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="607-615" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="616-624" color="#a6acb3" type="standard"/></Feature><Feature recentID="5" name="hypothetical protein" directionality="2" translationMW="333.45" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="82" maxFusedRunOn="82"><Segment range="616-624" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MIA"/></Q></Feature><Feature recentID="6" name="FTL_0126" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="616-624" color="#00e600" type="standard"/></Feature><Feature recentID="7" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="649-667" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="NLuc" directionality="2" translationMW="19250.08" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="82" maxFusedRunOn="82"><Segment range="85-606" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="AGVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRLCERILATG"/></Q></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="16-63" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="T7 terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2485-2532" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4371-4475" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2750-3338" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4301" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="3510-4301" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="4302-4370" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="14" name="firefly_luciferase" directionality="1" translationMW="60512.97" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="761-2410" color="#ffff99" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"/></Q></Feature><Feature recentID="15" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="743-760" color="#00e600" type="standard"/></Feature><Feature recentID="16" name="lipoprotein" directionality="1" translationMW="760.05" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2410" maxFusedRunOn="2410"><Segment range="743-760" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MKKIIK"/></Q></Feature><Feature recentID="17" name="tul4 UTR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="707-742" color="#a6acb3" type="standard"/></Feature><Feature recentID="18" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="688-706" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="707-707" color="#a6acb3" type="standard"/></Feature></Features>
  <<?xml version="1.0"?><Primers nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="KROL358  T7_amp_F" sequence="CTAGTGGTGCTAGCCAAAAAACCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-11-12T18:52:01Z"><BindingSite location="1-24" boundStrand="0" annealedBases="CTAGTGGTGCTAGCCAAAAAACCC" meltingTemperature="60"><Component hybridizedRange="1-24" bases="CTAGTGGTGCTAGCCAAAAAACCC"/></BindingSite><BindingSite location="2522-2531" boundStrand="1" annealedBases="CAAAAAACCC" meltingTemperature="29"><Component bases="CTAGTGGTGCTAGC"/><Component hybridizedRange="2522-2531" bases="CAAAAAACCC"/></BindingSite><BindingSite simplified="1" location="1-24" boundStrand="0" annealedBases="CTAGTGGTGCTAGCCAAAAAACCC" meltingTemperature="60"><Component hybridizedRange="1-24" bases="CTAGTGGTGCTAGCCAAAAAACCC"/></BindingSite><BindingSite simplified="1" location="2522-2531" boundStrand="1" annealedBases="CAAAAAACCC" meltingTemperature="29"><Component bases="CTAGTGGTGCTAGC"/><Component hybridizedRange="2522-2531" bases="CAAAAAACCC"/></BindingSite></Primer><Primer recentID="1" name="KROL363  T7_seq_1" sequence="GTTATTGTCTCATGAGCGGATACATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-11-12T19:32:18Z"><BindingSite location="4408-4433" boundStrand="0" annealedBases="GTTATTGTCTCATGAGCGGATACATA" meltingTemperature="56"><Component hybridizedRange="4408-4433" bases="GTTATTGTCTCATGAGCGGATACATA"/></BindingSite><BindingSite simplified="1" location="4408-4433" boundStrand="0" annealedBases="GTTATTGTCTCATGAGCGGATACATA" meltingTemperature="56"><Component hybridizedRange="4408-4433" bases="GTTATTGTCTCATGAGCGGATACATA"/></BindingSite></Primer><Primer recentID="2" name="KROL363 T7_seq_1" sequence="GTTATTGTCTCATGAGCGGATACATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2022-06-08T16:23:22Z"><BindingSite location="4408-4433" boundStrand="0" annealedBases="GTTATTGTCTCATGAGCGGATACATA" meltingTemperature="56"><Component hybridizedRange="4408-4433" bases="GTTATTGTCTCATGAGCGGATACATA"/></BindingSite><BindingSite simplified="1" location="4408-4433" boundStrand="0" annealedBases="GTTATTGTCTCATGAGCGGATACATA" meltingTemperature="56"><Component hybridizedRange="4408-4433" bases="GTTATTGTCTCATGAGCGGATACATA"/></BindingSite></Primer><Primer recentID="3" name="KROL539 firefly_luciferase_f&#10;" sequence="GCGCGTCGACGGTACCGCTCT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2022-07-11T18:17:19Z"><BindingSite location="667-683" boundStrand="0" annealedBases="GTCGACGGTACCGCTCT" meltingTemperature="58"><Component bases="GCGC"/><Component hybridizedRange="667-683" bases="GTCGACGGTACCGCTCT"/></BindingSite><BindingSite simplified="1" location="667-683" boundStrand="0" annealedBases="GTCGACGGTACCGCTCT" meltingTemperature="58"><Component bases="GCGC"/><Component hybridizedRange="667-683" bases="GTCGACGGTACCGCTCT"/></BindingSite></Primer><Primer recentID="4" name="KROL540 fLuc_seq_1&#10;" sequence="tcttttactgctatcattgtctacttaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2022-07-11T18:21:12Z"><BindingSite location="607-634" boundStrand="0" annealedBases="tcttttactgctatcattgtctacttaa" meltingTemperature="54"><Component hybridizedRange="607-634" bases="tcttttactgctatcattgtctacttaa"/></BindingSite><BindingSite simplified="1" location="607-634" boundStrand="0" annealedBases="tcttttactgctatcattgtctacttaa" meltingTemperature="54"><Component hybridizedRange="607-634" bases="tcttttactgctatcattgtctacttaa"/></BindingSite></Primer><Primer recentID="5" name="KROL361  pdpA_t7_salI_r" sequence="atatGTCGACTAATACGACTCACTATAGGagttatgttctaattaagtagacaatgat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2022-07-11T18:21:35Z"><BindingSite location="619-672" boundStrand="1" annealedBases="GTCGACTAATACGACTCACTATAGGagttatgttctaattaagtagacaatgat" meltingTemperature="63"><Component bases="atat"/><Component hybridizedRange="619-672" bases="GTCGACTAATACGACTCACTATAGGagttatgttctaattaagtagacaatgat"/></BindingSite><BindingSite simplified="1" location="619-672" boundStrand="1" annealedBases="GTCGACTAATACGACTCACTATAGGagttatgttctaattaagtagacaatgat" meltingTemperature="63"><Component bases="atat"/><Component hybridizedRange="619-672" bases="GTCGACTAATACGACTCACTATAGGagttatgttctaattaagtagacaatgat"/></BindingSite></Primer><Primer recentID="6" name="KROL323 pdpA_UTR_ext_f" sequence="tagctgtaaacatatagtcaatagataagtgcttatgtaaaatgactttagttatgttctaattaagtagacaatgata" dateAdded="2022-07-11T18:25:05Z"><BindingSite location="618-647" boundStrand="1" annealedBases="agttatgttctaattaagtagacaatgata" meltingTemperature="52"><Component bases="tagctgtaaacatatagtcaatagataagtgcttatgtaaaatgacttt"/><Component hybridizedRange="618-647" bases="agttatgttctaattaagtagacaatgata"/></BindingSite><BindingSite simplified="1" location="618-647" boundStrand="1" annealedBases="agttatgttctaattaagtagacaatgata" meltingTemperature="52"><Component bases="tagctgtaaacatatagtcaatagataagtgcttatgtaaaatgacttt"/><Component hybridizedRange="618-647" bases="agttatgttctaattaagtagacaatgata"/></BindingSite></Primer><Primer recentID="7" name="KROL467 pKR114_tul4_R" sequence="atatGCGGCCGCcttaattatttttttcattgtactcctatttttaaatattcacatatactc" dateAdded="2022-07-11T18:25:05Z"><BindingSite location="706-760" boundStrand="1" annealedBases="Ccttaattatttttttcattactcctatttttaaatattcacatatactc" meltingTemperature="53"><Component bases="atatGCGGCCG"/><Component hybridizedRange="742-760" bases="Ccttaattatttttttcat"/><Component bases="tg"/><Component hybridizedRange="706-736" bases="tactcctatttttaaatattcacatatactc"/></BindingSite><BindingSite simplified="1" location="706-760" boundStrand="1" annealedBases="Ccttaattatttttttcattactcctatttttaaatattcacatatactc" meltingTemperature="53"><Component bases="atatGCGGCCG"/><Component hybridizedRange="742-760" bases="Ccttaattatttttttcat"/><Component bases="tg"/><Component hybridizedRange="706-736" bases="tactcctatttttaaatattcacatatactc"/></BindingSite></Primer><Primer recentID="8" name="KROL318 LacZ_construct_f" sequence="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" dateAdded="2022-07-11T18:25:05Z"><BindingSite location="614-641" boundStrand="1" annealedBases="gttctaattaagtagacaatgatagcaG" meltingTemperature="55"><Component hybridizedRange="614-641" bases="gttctaattaagtagacaatgatagcaG"/><Component bases="CCATGATTACGGATTCACTGG"/></BindingSite><BindingSite simplified="1" location="614-641" boundStrand="1" annealedBases="gttctaattaagtagacaatgatagcaG" meltingTemperature="55"><Component hybridizedRange="614-641" bases="gttctaattaagtagacaatgatagcaG"/><Component bases="CCATGATTACGGATTCACTGG"/></BindingSite></Primer><Primer recentID="9" name="KROL350 tul4_6aa_NotI" sequence="atatGCGGCCGCcttaattatttttttcatatagatactcct" dateAdded="2022-07-11T18:25:05Z"><BindingSite location="730-760" boundStrand="1" annealedBases="Ccttaattatttttttcatatagatactcct" meltingTemperature="53"><Component bases="atatGCGGCCG"/><Component hybridizedRange="730-760" bases="Ccttaattatttttttcatatagatactcct"/></BindingSite><BindingSite simplified="1" location="730-760" boundStrand="1" annealedBases="Ccttaattatttttttcatatagatactcct" meltingTemperature="53"><Component bases="atatGCGGCCG"/><Component hybridizedRange="730-760" bases="Ccttaattatttttttcatatagatactcct"/></BindingSite></Primer><Primer recentID="10" name="KROL349 pdpA_6aa_NotI" sequence="atatGCGGCCGCatcttttactgctatcattgtctactta" dateAdded="2022-07-11T18:25:05Z"><BindingSite location="602-633" boundStrand="0" annealedBases="CCGCatcttttactgctatcattgtctactta" meltingTemperature="60"><Component bases="atatGCGG"/><Component hybridizedRange="602-633" bases="CCGCatcttttactgctatcattgtctactta"/></BindingSite><BindingSite simplified="1" location="602-633" boundStrand="0" annealedBases="CCGCatcttttactgctatcattgtctactta" meltingTemperature="60"><Component bases="atatGCGG"/><Component hybridizedRange="602-633" bases="CCGCatcttttactgctatcattgtctactta"/></BindingSite></Primer><Primer recentID="11" name="KROL329 pKR70_forward_frag2" sequence="taatcgatttagttatgttctaattaagtagacaatgat" dateAdded="2022-07-11T18:25:05Z"><BindingSite location="619-647" boundStrand="1" annealedBases="agttatgttctaattaagtagacaatgat" meltingTemperature="53"><Component bases="taatcgattt"/><Component hybridizedRange="619-647" bases="agttatgttctaattaagtagacaatgat"/></BindingSite><BindingSite simplified="1" location="619-647" boundStrand="1" annealedBases="agttatgttctaattaagtagacaatgat" meltingTemperature="53"><Component bases="taatcgattt"/><Component hybridizedRange="619-647" bases="agttatgttctaattaagtagacaatgat"/></BindingSite></Primer><Primer recentID="12" name="KROL368 OE_min10mut_F" sequence="ttatgtCaaGtgactttagttatgttctaattaagtaga" dateAdded="2022-07-11T18:25:05Z"><BindingSite location="626-647" boundStrand="1" annealedBases="agttatgttctaattaagtaga" meltingTemperature="44"><Component bases="ttatgtCaaGtgacttt"/><Component hybridizedRange="626-647" bases="agttatgttctaattaagtaga"/></BindingSite><BindingSite simplified="1" location="626-647" boundStrand="1" annealedBases="agttatgttctaattaagtaga" meltingTemperature="44"><Component bases="ttatgtCaaGtgacttt"/><Component hybridizedRange="626-647" bases="agttatgttctaattaagtaga"/></BindingSite></Primer><Primer recentID="13" name="KROL483 tul4UTR_OE_PCR_F" sequence="agaataatccgagtatatgtgaatatttaaaaataggag" dateAdded="2022-07-11T18:25:05Z"><BindingSite location="706-734" boundStrand="0" annealedBases="gagtatatgtgaatatttaaaaataggag" meltingTemperature="51"><Component bases="agaataatcc"/><Component hybridizedRange="706-734" bases="gagtatatgtgaatatttaaaaataggag"/></BindingSite><BindingSite simplified="1" location="706-734" boundStrand="0" annealedBases="gagtatatgtgaatatttaaaaataggag" meltingTemperature="51"><Component bases="agaataatcc"/><Component hybridizedRange="706-734" bases="gagtatatgtgaatatttaaaaataggag"/></BindingSite></Primer><Primer recentID="14" name="KROL430 pKR102_seq" sequence="cttaattatttttttcatatagatactcct" dateAdded="2022-07-11T18:25:05Z"><BindingSite location="730-759" boundStrand="1" annealedBases="cttaattatttttttcatatagatactcct" meltingTemperature="50"><Component hybridizedRange="730-759" bases="cttaattatttttttcatatagatactcct"/></BindingSite><BindingSite simplified="1" location="730-759" boundStrand="1" annealedBases="cttaattatttttttcatatagatactcct" meltingTemperature="50"><Component hybridizedRange="730-759" bases="cttaattatttttttcatatagatactcct"/></BindingSite></Primer><Primer recentID="15" name="KROL518 NLuc_F" sequence="ATATGTCGACTAATACGACTCACTATAGG" dateAdded="2022-07-11T18:25:05Z"><BindingSite location="648-672" boundStrand="1" annealedBases="GTCGACTAATACGACTCACTATAGG" meltingTemperature="56"><Component bases="ATAT"/><Component hybridizedRange="648-672" bases="GTCGACTAATACGACTCACTATAGG"/></BindingSite><BindingSite location="667-705" boundStrand="0" annealedBases="GTCGACTAATACGACTCACTATAGG" meltingTemperature="46"><Component bases="ATAT"/><Component hybridizedRange="667-672" bases="GTCGAC"/><Component hybridizedRange="687-705" bases="TAATACGACTCACTATAGG"/></BindingSite><BindingSite simplified="1" location="648-672" boundStrand="1" annealedBases="GTCGACTAATACGACTCACTATAGG" meltingTemperature="56"><Component bases="ATAT"/><Component hybridizedRange="648-672" bases="GTCGACTAATACGACTCACTATAGG"/></BindingSite><BindingSite simplified="1" location="687-705" boundStrand="0" annealedBases="TAATACGACTCACTATAGG" meltingTemperature="46"><Component bases="ATATGTCGAC"/><Component hybridizedRange="687-705" bases="TAATACGACTCACTATAGG"/></BindingSite></Primer><Primer recentID="16" name="KROL371 pdpA_6aa_NotI_shorter" sequence="atatGCGGCCGCatcttttactgctatc" dateAdded="2022-07-11T18:25:05Z"><BindingSite location="602-621" boundStrand="0" annealedBases="CCGCatcttttactgctatc" meltingTemperature="54"><Component bases="atatGCGG"/><Component hybridizedRange="602-621" bases="CCGCatcttttactgctatc"/></BindingSite><BindingSite simplified="1" location="602-621" boundStrand="0" annealedBases="CCGCatcttttactgctatc" meltingTemperature="54"><Component bases="atatGCGG"/><Component hybridizedRange="602-621" bases="CCGCatcttttactgctatc"/></BindingSite></Primer><Primer recentID="17" name="KROL519 Nluc_R" sequence="ATATCAGCTGTTAACCGGTCGCCAGG" dateAdded="2022-07-11T18:25:05Z"><BindingSite location="75-96" boundStrand="0" annealedBases="CAGCTGTTAACCGGTCGCCAGG" meltingTemperature="64"><Component bases="ATAT"/><Component hybridizedRange="75-96" bases="CAGCTGTTAACCGGTCGCCAGG"/></BindingSite><BindingSite simplified="1" location="75-96" boundStrand="0" annealedBases="CAGCTGTTAACCGGTCGCCAGG" meltingTemperature="64"><Component bases="ATAT"/><Component hybridizedRange="75-96" bases="CAGCTGTTAACCGGTCGCCAGG"/></BindingSite></Primer><Primer recentID="18" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2022-07-11T18:25:05Z"><BindingSite location="2610-2630" boundStrand="1" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="2610-2630" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="2610-2630" boundStrand="1" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="2610-2630" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="19" name="KROL541 fLuc_seq_2&#10;" sequence="CCGATTATCCAGAAAATCATTATTATGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2022-07-11T18:28:08Z"><BindingSite location="1186-1213" boundStrand="0" annealedBases="CCGATTATCCAGAAAATCATTATTATGG" meltingTemperature="54"><Component hybridizedRange="1186-1213" bases="CCGATTATCCAGAAAATCATTATTATGG"/></BindingSite><BindingSite simplified="1" location="1186-1213" boundStrand="0" annealedBases="CCGATTATCCAGAAAATCATTATTATGG" meltingTemperature="54"><Component hybridizedRange="1186-1213" bases="CCGATTATCCAGAAAATCATTATTATGG"/></BindingSite></Primer><Primer recentID="20" name="KROL542 fLuc_seq_3&#10;" sequence="TCTAAAGAAGTAGGTGAAGCTGTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2022-07-11T18:29:22Z"><BindingSite location="1714-1738" boundStrand="0" annealedBases="TCTAAAGAAGTAGGTGAAGCTGTGG" meltingTemperature="58"><Component hybridizedRange="1714-1738" bases="TCTAAAGAAGTAGGTGAAGCTGTGG"/></BindingSite><BindingSite simplified="1" location="1714-1738" boundStrand="0" annealedBases="TCTAAAGAAGTAGGTGAAGCTGTGG" meltingTemperature="58"><Component hybridizedRange="1714-1738" bases="TCTAAAGAAGTAGGTGAAGCTGTGG"/></BindingSite></Primer><Primer recentID="21" name="KROL672 pdpA_perfectSD_R" sequence="atatGTCGACTAATACGACTCACTATAGGagttatgttctaaggaggtagacaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2023-05-26T15:48:06Z"><BindingSite location="621-672" boundStrand="1" annealedBases="GTCGACTAATACGACTCACTATAGGagttatgttctaagtagacaatg" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="635-672" bases="GTCGACTAATACGACTCACTATAGGagttatgttctaa"/><Component bases="ggag"/><Component hybridizedRange="621-630" bases="gtagacaatg"/></BindingSite><BindingSite simplified="1" location="621-672" boundStrand="1" annealedBases="GTCGACTAATACGACTCACTATAGGagttatgttctaagtagacaatg" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="635-672" bases="GTCGACTAATACGACTCACTATAGGagttatgttctaa"/><Component bases="ggag"/><Component hybridizedRange="621-630" bases="gtagacaatg"/></BindingSite></Primer></Primers>
  g<Notes>
<UUID>31b1c2e7-b420-4130-b814-18706589011b</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="18:9:59">2022.7.11</Created>
<LastModified UTC="15:48:6">2023.5.26</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         6+ Cutters                                                                                                   d     CHO               E              Y? P                                                                                                                                                              )<?xml version="1.0"?><CustomEnzymeSets/>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
