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   )YQZ	 - C 		

)  IZ  , HWXYNOP		

  &  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                   
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
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   3   )         2   4   	            )                                                                                                                                                                        
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6)l=Ţį!Z*Z85_̽ekX[gܯIɷG^^n`O9ME{	6yrjqBH,)3`̥dC    # (  %g    YZ                  9=UGC@)@o֍8e|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_90l   D   I   R   T   ~    7zXZ  ִF !   t/t] 3D].?I*ә_.ZvќP,Vj,ު~mC<ґ8hlFOlY.k(DW`!
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B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    D   I   R   T   ~    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="81-87" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="15-62" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="108-126" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="125-125" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-612" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="163-612" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="163-612" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-253" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="314-334" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-127" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="613-662" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="233-252" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="233-252" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="233-252" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="233-252" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="313-333" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="313-333" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="313-333" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="313-333" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:47:54">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  Uʑ 9Z4b	gdYUIUVZSmlЩ18{);NFxӞxMN8nS׍TY^eYT׉gYeF`E
#gS֞RcQqz^%Ҕ%$RNXQIl놵fZJUU;   ;   @   I   K   u    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-78" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="99-117" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="116-116" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="118-603" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="154-603" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="154-603" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="225-244" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="305-325" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="118-118" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="604-653" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="224-243" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="224-243" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="224-243" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="224-243" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="304-324" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="304-324" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="304-324" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="304-324" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:47:10">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  Uʑ 9Z4ᙋ'륜{yf!fVA&aUYiNꛩB@ cU:ӕ;GNy58㉹O^5RRezAeS^&gMePR%uC+aNZyIEUxJSP^	H9bJuE%VjZ՚j*UU`C$   ;   @   I   J   t    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-77" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-116" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-115" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="117-602" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="153-602" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="153-602" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="224-243" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="304-324" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="117-117" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="603-652" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="223-242" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="223-242" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="223-242" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="223-242" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="303-323" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="303-323" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="303-323" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="303-323" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:33">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  Uʑ 9Z4ᘱ|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   r    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-75" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="96-114" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-113" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-600" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="151-600" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="151-600" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="222-241" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="302-322" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-115" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-650" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="221-240" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="221-240" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="221-240" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="221-240" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="301-321" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="301-321" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="301-321" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="301-321" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:28">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  Uʑ 9Z4|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   n    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  X<?xml version="1.0"?><Features recycledIDs="10,11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="92-110" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="109-109" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="111-596" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="147-596" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="147-596" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="218-237" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="298-318" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="111-111" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="597-646" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="217-236" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="217-236" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="217-236" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="217-236" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="297-317" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="297-317" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="297-317" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="297-317" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:14">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  13     Q          Uʑ 9Z4JHVYaUjUgꩶTZjzbފj*E($4Ji{Yjߩ%Qj֔e-ehijbD%&aaeھZ&QOdI}VƄ냦ZQje:][)}TzzAJFe؋G&MjfrީiJ(IVY:fS>U*.JQ%IEDnվ$Z&m:th`a&[H)RݴvU*i4ednjqKinud=mYVM;RUVU^$VV: ɪiEUTf۔ꚕ9F
/T -h{Ev	YkTY*=`UnUNmYe
dKmT<떪aR%)f(gUJjC%4flCNZ*yJIGmj@NZWJZZ1.JYeknb[sYj>Rnj(IibZYfNijM^EBڙzJ^Zb虩[Jf9v^ɤ|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   G  
/  
j    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="21"><Feature recentID="20" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="122-2608" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="19" name="FTL_0126" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="122-2584" color="#00e600" type="standard"/></Feature><Feature recentID="18" name="hypothetical protein" directionality="2" translationMW="95341.69" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="122-2584" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2608-2608" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="16" name="pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="122-2584" color="#a6acb3" type="standard"/></Feature><Feature recentID="15" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2609-2627" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2610-2610" color="#a6acb3" type="standard"/></Feature><Feature recentID="13" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-121" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="12" name="FTL_0125" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="72-117" color="#00e600" type="standard"/></Feature><Feature recentID="11" name="hypothetical protein" directionality="2" translationMW="1785.21" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="28" maxFusedRunOn="28"><Segment range="72-117" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="pdpB" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-117" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2666" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2665-2665" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2667-3152" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2703-3152" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2703-3152" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2774-2793" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="2854-2874" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2667-2667" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3153-3202" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P823 DFPI_FR1R2" sequence="atatGCGGCCGCtgctatcattgtctacttaattagaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2574-2602" boundStrand="0" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="2574-2602" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite><BindingSite simplified="1" location="2574-2602" boundStrand="0" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="2574-2602" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite></Primer><Primer recentID="1" name="KROL311  pdpA_qPCR_R1" sequence="cacttgatctttgtatcgcactatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2133-2157" boundStrand="0" annealedBases="cacttgatctttgtatcgcactatc" meltingTemperature="57"><Component hybridizedRange="2133-2157" bases="cacttgatctttgtatcgcactatc"/></BindingSite><BindingSite simplified="1" location="2133-2157" boundStrand="0" annealedBases="cacttgatctttgtatcgcactatc" meltingTemperature="57"><Component hybridizedRange="2133-2157" bases="cacttgatctttgtatcgcactatc"/></BindingSite></Primer><Primer recentID="2" name="KROL310  pdpA_qPCR_F1" sequence="cttgagctccctcatgattaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2190-2211" boundStrand="1" annealedBases="cttgagctccctcatgattaca" meltingTemperature="57"><Component hybridizedRange="2190-2211" bases="cttgagctccctcatgattaca"/></BindingSite><BindingSite simplified="1" location="2190-2211" boundStrand="1" annealedBases="cttgagctccctcatgattaca" meltingTemperature="57"><Component hybridizedRange="2190-2211" bases="cttgagctccctcatgattaca"/></BindingSite></Primer><Primer recentID="3" name="KROL312  pdpA_qPCR_F2" sequence="aatcatgagggagctcaagaatta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2193-2216" boundStrand="0" annealedBases="aatcatgagggagctcaagaatta" meltingTemperature="55"><Component hybridizedRange="2193-2216" bases="aatcatgagggagctcaagaatta"/></BindingSite><BindingSite simplified="1" location="2193-2216" boundStrand="0" annealedBases="aatcatgagggagctcaagaatta" meltingTemperature="55"><Component hybridizedRange="2193-2216" bases="aatcatgagggagctcaagaatta"/></BindingSite></Primer><Primer recentID="4" name="KROL313  pdpA_qPCR_R2" sequence="cgacaaactataaagactct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2269-2288" boundStrand="1" annealedBases="cgacaaactataaagactct" meltingTemperature="50"><Component hybridizedRange="2269-2288" bases="cgacaaactataaagactct"/></BindingSite><BindingSite simplified="1" location="2269-2288" boundStrand="1" annealedBases="cgacaaactataaagactct" meltingTemperature="50"><Component hybridizedRange="2269-2288" bases="cgacaaactataaagactct"/></BindingSite></Primer><Primer recentID="5" name="KROL314  pdpA_qPCR_F3" sequence="acccaatatatcgtgaaaggct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2067-2088" boundStrand="0" annealedBases="acccaatatatcgtgaaaggct" meltingTemperature="56"><Component hybridizedRange="2067-2088" bases="acccaatatatcgtgaaaggct"/></BindingSite><BindingSite simplified="1" location="2067-2088" boundStrand="0" annealedBases="acccaatatatcgtgaaaggct" meltingTemperature="56"><Component hybridizedRange="2067-2088" bases="acccaatatatcgtgaaaggct"/></BindingSite></Primer><Primer recentID="6" name="KROL315  pdpA_qPCR_R3" sequence="gatagtgcgatacaaagatcaagtg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2133-2157" boundStrand="1" annealedBases="gatagtgcgatacaaagatcaagtg" meltingTemperature="56"><Component hybridizedRange="2133-2157" bases="gatagtgcgatacaaagatcaagtg"/></BindingSite><BindingSite simplified="1" location="2133-2157" boundStrand="1" annealedBases="gatagtgcgatacaaagatcaagtg" meltingTemperature="56"><Component hybridizedRange="2133-2157" bases="gatagtgcgatacaaagatcaagtg"/></BindingSite></Primer><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="2773-2792" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="2773-2792" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="2773-2792" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="2773-2792" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="2853-2873" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="2853-2873" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="2853-2873" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="2853-2873" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:44:19">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  	l7zXZ  ִF !   t/V	+] ˇf{3(1v%f M*	mj)p80*i	NY]QF7&!nBvgYF֍I> r񗱑榰|"IO_z`Ĉ18$릪s$pw?<@a#WnF?\A9?L0
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I&:MgFe~v#HMW ;( qjߢvlE9/c'JךFLO%lmjNM!.{Dd>MzJ3^I/OsۼkM2fNp(i)BglЇ[gA!nRأ4o<A<'tiŞnB0%zKPT$iǐWsQ:V*/d>1J;<ࢅcTbN	3PS U8Wf?D0nJ#Auym8)׼(ym:^5RόD1{u'li,銴9Lsa"\|^-fִ]J8hABnLxfL
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:V_!hJ1-+	ܙlzxp\q:gdAOn7һ?F{JOƽ׶	-xn_DKK
ymætnctqEfO~vKbJO`l緒.4,ڥAXs9/0;g|H#2Y0psLؿxY^LL![0ܰGeGKIum8yJ?AUKh[DdnSTNu5/aPpf^2?\XWf{kԻQ1(Lèyhٷsdu8|14%Sp@玺 "-mȹTL$=hb6GGʕxl#<~\&kwղ6gfg#NF^Q੦||r$<q 5d'`|o*x#6FF`}=\h9M׶WdCu60z^,h#[%6;V	.d 8q
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]명P#6db&fzkD}-Bn99^,_&d-8BZ=H=0_DvIAhEb]SN֎3XˑaOd7YJ56߼	MHWE=Ӊux0#B Ő{@	y@dd8   GW  Yg    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  .<?xml version="1.0"?><Features nextValidID="22"><Feature recentID="0" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="3160" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="2369-3160" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="3161-3229" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="1" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1609-2197" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3230-3334" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="T7 terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1344-1391" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="707-707" color="#a6acb3" type="standard"/></Feature><Feature recentID="5" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="894-914" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="6" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="814-833" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="7" name="lipoprotein" directionality="1" translationMW="15771.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="743-1192" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MKKIIKLSLLSLSIAGLASCSTLGLGGSDDAKASAKDTAAAQTATTEQAAAVSKPTAKVSLNKLGQDKIKATVYTAYNNNPQGSVRLQWQAPEGSKCHDTSFPITKYAEKNDKTWATVTVKQGNNFCSGKWTANVVYDKEVIASDSINI*"/></Q></Feature><Feature recentID="8" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="743-1192" color="#00e600" type="standard"/></Feature><Feature recentID="9" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="705-705" color="#a6acb3" type="standard"/></Feature><Feature recentID="10" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="688-706" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="16-63" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="NLuc" directionality="2" translationMW="19250.08" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="82" maxFusedRunOn="82"><Segment range="85-606" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="AGVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRLCERILATG"/></Q></Feature><Feature recentID="13" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="649-667" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="FTL_0126" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="616-624" color="#00e600" type="standard"/></Feature><Feature recentID="15" name="hypothetical protein" directionality="2" translationMW="333.45" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="82" maxFusedRunOn="82"><Segment range="616-624" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MIA"/></Q></Feature><Feature recentID="16" name="pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="616-624" color="#a6acb3" type="standard"/></Feature><Feature recentID="17" name="FTL_0126" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="607-615" color="#00e600" type="standard"/></Feature><Feature recentID="18" name="hypothetical protein" directionality="2" translationMW="360.41" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="4" maxRunOn="82" maxFusedRunOn="82"><Segment range="607-615" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="VKD"/></Q></Feature><Feature recentID="19" name="pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="607-615" color="#a6acb3" type="standard"/></Feature><Feature recentID="20" name="pdpA UTR" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="625-648" color="#a6acb3" type="standard"/></Feature><Feature recentID="21" name="tul4-UTR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="707-742" color="#a6acb3" type="standard"/></Feature></Features>
  :<?xml version="1.0"?><Primers nextValidID="11"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="813-832" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="813-832" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="813-832" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="813-832" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="1" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="893-913" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="55"><Component hybridizedRange="893-913" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="893-913" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="55"><Component hybridizedRange="893-913" bases="ttgcagttggcttagatacag"/></BindingSite></Primer><Primer recentID="2" name="KROL358  T7_amp_F" sequence="CTAGTGGTGCTAGCCAAAAAACCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-11-12T18:52:01Z"><BindingSite location="1-24" boundStrand="0" annealedBases="CTAGTGGTGCTAGCCAAAAAACCC" meltingTemperature="60"><Component hybridizedRange="1-24" bases="CTAGTGGTGCTAGCCAAAAAACCC"/></BindingSite><BindingSite simplified="1" location="1-24" boundStrand="0" annealedBases="CTAGTGGTGCTAGCCAAAAAACCC" meltingTemperature="60"><Component hybridizedRange="1-24" bases="CTAGTGGTGCTAGCCAAAAAACCC"/></BindingSite></Primer><Primer recentID="3" name="KROL359  T7_amp_R" sequence="GATCGATCCGCTAGCCTCGAGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-11-12T18:58:35Z"><BindingSite location="1253-1283" boundStrand="1" annealedBases="GATCGATCCGCTAGCCTCGAGC" meltingTemperature="53"><Component hybridizedRange="1277-1283" bases="GATCGAT"/><Component hybridizedRange="1253-1267" bases="CCGCTAGCCTCGAGC"/></BindingSite><BindingSite simplified="1" location="1253-1267" boundStrand="1" annealedBases="CCGCTAGCCTCGAGC" meltingTemperature="53"><Component bases="GATCGAT"/><Component hybridizedRange="1253-1267" bases="CCGCTAGCCTCGAGC"/></BindingSite></Primer><Primer recentID="4" name="KROL363  T7_seq_1" sequence="GTTATTGTCTCATGAGCGGATACATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-11-12T19:32:18Z"><BindingSite location="3267-3292" boundStrand="0" annealedBases="GTTATTGTCTCATGAGCGGATACATA" meltingTemperature="56"><Component hybridizedRange="3267-3292" bases="GTTATTGTCTCATGAGCGGATACATA"/></BindingSite><BindingSite simplified="1" location="3267-3292" boundStrand="0" annealedBases="GTTATTGTCTCATGAGCGGATACATA" meltingTemperature="56"><Component hybridizedRange="3267-3292" bases="GTTATTGTCTCATGAGCGGATACATA"/></BindingSite></Primer><Primer recentID="5" name="KROL364  T7_seq_2" sequence="aacttgggcaactgtaacagttaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-11-12T19:33:45Z"><BindingSite location="1080-1105" boundStrand="0" annealedBases="aacttgggcaactgtaacagttaagc" meltingTemperature="60"><Component hybridizedRange="1080-1105" bases="aacttgggcaactgtaacagttaagc"/></BindingSite><BindingSite simplified="1" location="1080-1105" boundStrand="0" annealedBases="aacttgggcaactgtaacagttaagc" meltingTemperature="60"><Component hybridizedRange="1080-1105" bases="aacttgggcaactgtaacagttaagc"/></BindingSite></Primer><Primer recentID="6" name="KROL359 T7_amp_R" sequence="GATCGATCCGCTAGCCTCGAGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2022-06-08T16:12:30Z"><BindingSite location="1253-1283" boundStrand="1" annealedBases="GATCGATCCGCTAGCCTCGAGC" meltingTemperature="53"><Component hybridizedRange="1277-1283" bases="GATCGAT"/><Component hybridizedRange="1253-1267" bases="CCGCTAGCCTCGAGC"/></BindingSite><BindingSite simplified="1" location="1253-1267" boundStrand="1" annealedBases="CCGCTAGCCTCGAGC" meltingTemperature="53"><Component bases="GATCGAT"/><Component hybridizedRange="1253-1267" bases="CCGCTAGCCTCGAGC"/></BindingSite></Primer><Primer recentID="7" name="KROL363 T7_seq_1" sequence="GTTATTGTCTCATGAGCGGATACATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2022-06-08T16:23:22Z"><BindingSite location="3267-3292" boundStrand="0" annealedBases="GTTATTGTCTCATGAGCGGATACATA" meltingTemperature="56"><Component hybridizedRange="3267-3292" bases="GTTATTGTCTCATGAGCGGATACATA"/></BindingSite><BindingSite simplified="1" location="3267-3292" boundStrand="0" annealedBases="GTTATTGTCTCATGAGCGGATACATA" meltingTemperature="56"><Component hybridizedRange="3267-3292" bases="GTTATTGTCTCATGAGCGGATACATA"/></BindingSite></Primer><Primer recentID="8" name="KROL628 NLucF" sequence="atatGTTAACCGGTCGCCAGGATACGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2023-03-24T22:07:18Z"><BindingSite location="79-102" boundStrand="0" annealedBases="tGTTAACCGGTCGCCAGGATACGT" meltingTemperature="65"><Component bases="ata"/><Component hybridizedRange="79-102" bases="tGTTAACCGGTCGCCAGGATACGT"/></BindingSite><BindingSite simplified="1" location="79-102" boundStrand="0" annealedBases="tGTTAACCGGTCGCCAGGATACGT" meltingTemperature="65"><Component bases="ata"/><Component hybridizedRange="79-102" bases="tGTTAACCGGTCGCCAGGATACGT"/></BindingSite></Primer><Primer recentID="9" name="KROL629 NLucR" sequence="atatGCGGCCGCatagatactcctatttttaaatattcacat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2023-03-24T22:10:03Z"><BindingSite location="712-741" boundStrand="1" annealedBases="atagatactcctatttttaaatattcacat" meltingTemperature="52"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="712-741" bases="atagatactcctatttttaaatattcacat"/></BindingSite><BindingSite simplified="1" location="712-741" boundStrand="1" annealedBases="atagatactcctatttttaaatattcacat" meltingTemperature="52"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="712-741" bases="atagatactcctatttttaaatattcacat"/></BindingSite></Primer><Primer recentID="10" name="KROL350 tul4_6aa_NotI" sequence="atatGCGGCCGCcttaattatttttttcatatagatactcct" dateAdded="2023-03-27T22:30:40Z"><BindingSite location="730-759" boundStrand="1" annealedBases="cttaattatttttttcatatagatactcct" meltingTemperature="50"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="730-759" bases="cttaattatttttttcatatagatactcct"/></BindingSite><BindingSite simplified="1" location="730-759" boundStrand="1" annealedBases="cttaattatttttttcatatagatactcct" meltingTemperature="50"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="730-759" bases="cttaattatttttttcatatagatactcct"/></BindingSite></Primer></Primers>
  f<Notes>
<UUID>8d917676-1bcf-4583-9fcb-1f8f22aec698</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="19:16:25">2020.11.12</Created>
<LastModified UTC="22:30:40">2023.3.27</LastModified>
<CreatedBy>kramseylab</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         Unsaved Enzyme Set                                                                                           d     CHO                E              Y? P                                                                                                                                                              <?xml version="1.0"?><CustomEnzymeSets><CustomEnzymeSet type="2" name="Unsaved Enzyme Set" enzymeNames="PvuII SalI XhoI"/></CustomEnzymeSets>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
