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                                    &                                                         $                     	                                                                                                                  Z   )   "         !                              	                                                                                 	                                 $                           	                                          (                           1                  	                                                                     
                                          %                     	      
                           	                                                      	                           	
 b l93		


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&Rk`?!	C
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r* 	
 6Lb 
  Sb	{ipy(>   o{v`{		BImw   j 9N
L #W	-Tg	<		)
=

Hq*[.   za_z	Ax1 efyfB[\ 			<


'()?@./ . g.C]	

+0 / Q/
$ 3 bfhIJ^
^] 8f7D	

e17A qai\
o
,	 -
:	
 w6
E 0 M CQT RZ0
% '5Mq	M    g~H9N
:
D {,eiJr_~	

C_`A$o 4 c5YK	

  K#CS

C ^ & p 9g	
4(# *  8	
f8 Y_Odg#Z
L2  X3E	
8B  lj~-	p
- 7    	
_  b*	+Gt			e G rH]
Q a  k2	^		


P  IZ8	T		/t! n.LQ;	{l  J`WO		"
%
 l   Qx_o> FG		7
.u- # \ x$F
 MDl`
W* N Dr


dZR S[*sh 1 ,U+B		m
uJg&<v K Rc!(b1  v&p(	:<N  '0>V
f
3N
  N6	
J  xr	/	g	k		r$s = 8:b	x		;Ps'   <IQ		
O
h
;  x  dE8      h	!u' |1s	`% (2EKXf"`)		w
|


YNB%    fj|1@Gc  )u-L		c



DTacp 5-CY 9 }ZA~n		
 5d6xwL
F 5 n  A	p)|			X

^O	  LG$DT
r {Da
 
!   


!F_ q !R{

T B :Vv		
A

zo '  		
)x$ }  h5t		5`|g 
 49M	g9  +  -jlb	 Y :	
Y Zk%$
M3X 	 i R	o	5Ka  d,Z	(
;
--w a`PehI[
@\ P,Yv49LL   +X8
B9@#C X^f	
3'"7 F   kF	    m_K1	S		.k  Pk_
t J al
q

fu   u/.	j	9#I w     w	:&&  t00		
M 8 m  4}o(m		]   z
	` A  
u				Sf	  3,	
Xa cH	n  +ue8!?  I ^JR	i	^
Hj v  s9% tR H u I8#$  )   GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
               	                     
                                 	                                                            	                                                   F   E         8   4   !            9                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
     	       
           	                    	                 F E   8 4 !    9   Z(  Z  Z  Z  ]P  `  eV((S	l

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KanCass_R&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="RP4-OriT-OriR6K" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3845-4618" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;RP4-OriT-OriR6K&lt;br/&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="RBS" type="RBS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2758-2763" color="#a6acb3" type="standard"/><Q name="note"><V text="RBS"/></Q></Feature><Feature recentID="5" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2736-2772" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P581 PpSD26_R1"/></Q></Feature><Feature recentID="6" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2544-2574" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P580 PpSD26_F1"/></Q></Feature><Feature recentID="7" name="-10 signal" type="-10_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2607-2608" color="#a6acb3" type="standard"/></Feature><Feature recentID="8" name="-35 signal" type="-35_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2586-2591" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="-10 signal" type="-10_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2610-2615" color="#a6acb3" type="standard"/></Feature><Feature recentID="10" name="MmeI site" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2532-2537" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  frag"/></Q><Q name="note"><V text="MmeI"/></Q></Feature><Feature recentID="11" name="G-&gt;T" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2532-2532" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  frag"/></Q><Q name="note"><V text="G-&gt;T creates MmeI site"/></Q></Feature><Feature recentID="12" name="MmeI IR_R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2515-2543" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  frag"/></Q><Q name="note"><V text="MmeI IR_R"/></Q></Feature><Feature recentID="13" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2249-2273" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P501 Seq_pSam_Bt2_5"/></Q></Feature><Feature recentID="14" name="frag" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2495-2514" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  frag"/></Q><Q name="note"><V text="P7"/></Q></Feature><Feature recentID="15" name="BioSamA" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2494-2519" color="#a020f0" type="standard"/><Q name="note"><V text="BioSamA"/></Q></Feature><Feature recentID="16" name="terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2442-2469" color="#ffffff" type="standard"/><Q name="note"><V text="E. coli rrnB terminator T2"/></Q></Feature><Feature recentID="17" name="terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2267-2310" color="#ffffff" type="standard"/><Q name="note"><V text="E. coli rrnB terminator T1"/></Q></Feature><Feature recentID="18" name="frag" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2237-2480" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  frag"/></Q><Q name="note"><V text="3957 to 4200 of pFW11"/></Q></Feature><Feature recentID="19" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1828-1850" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P443 pEX_kanR_R"/></Q></Feature><Feature recentID="20" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1880-1904" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="21" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1556-1580" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P442 pEX_kanR_F"/></Q></Feature><Feature recentID="22" name="groES promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1163-1228" color="#ffffff" type="standard"/><Segment range="1229-1400" color="#ffffff" type="standard"/><Q name="note"><V text="groES promoter from LVS"/></Q></Feature><Feature recentID="23" name="transposon" type="mobile_element" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1078-1228" color="#a6acb3" type="standard"/><Segment range="1229-2543" color="#a6acb3" type="standard"/><Q name="mobile_element_type"><V predef="transposon"/></Q><Q name="note"><V text="transposon"/></Q></Feature><Feature recentID="24" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4526-4550" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P505 Seq_pSam_Bt2_9"/></Q></Feature><Feature recentID="25" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3739-3766" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P504 Seq_pSam_Bt2_8"/></Q></Feature><Feature recentID="26" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3188-3212" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P503 Seq_pSam_Bt2_7"/></Q></Feature><Feature recentID="27" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="593-613" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P498 Seq_pSam_Bt2_2"/></Q></Feature><Feature recentID="28" name="mariner transposase himar1C9" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2770-3816" color="#a6acb3" type="standard"/><Q name="note"><V text="mariner transposase himar1C9"/></Q></Feature><Feature recentID="29" name="NdeI" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2767-2772" color="#a6acb3" type="standard"/><Q name="note"><V text="NdeI"/></Q></Feature><Feature recentID="30" name="MluI" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1062-1067" color="#a6acb3" type="standard"/><Q name="note"><V text="MluI"/></Q></Feature><Feature recentID="31" name="promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="896-902" color="#ffffff" type="standard"/><Q name="note"><V text="P3"/></Q></Feature><Feature recentID="32" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="792" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="1-792" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="793-861" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="33" name="KanR" directionality="1" translationMW="30979.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1401-2216" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;div&gt;aphA1 from Tn903&lt;/div&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature recentID="34" name="R6K γ ori" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3849-4237" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;γ replication origin from &lt;i&gt;E. coli&lt;/i&gt; plasmid R6K; requires the R6K initiator protein pi for replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4352-4461" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="36" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="862-966" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="rrnB T1 terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2264-2350" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="Added" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1112-1119" color="#a6acb3" type="standard"/></Feature><Feature recentID="39" name="frag" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1107-1134" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  frag"/></Q><Q name="note"><V text="P7"/></Q></Feature><Feature recentID="40" name="BioSamA" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1102-1135" color="#a020f0" type="standard"/><Q name="note"><V text="BioSamA"/></Q></Feature><Feature recentID="41" name="MmeI site" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="1084-1089" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  frag"/></Q><Q name="note"><V text="MmeI"/></Q></Feature><Feature recentID="42" name="MmeI IR_L" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1078-1106" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  frag"/></Q><Q name="note"><V text="MmeI IR_L"/></Q></Feature></Features>
  J<?xml version="1.0"?><Primers nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="P497 Seq_pSam_Bt2_1" sequence="TACCAATGCTTAATCAGTGAGGCAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1-25" boundStrand="0" annealedBases="TACCAATGCTTAATCAGTGAGGCAC" meltingTemperature="59"><Component hybridizedRange="1-25" bases="TACCAATGCTTAATCAGTGAGGCAC"/></BindingSite><BindingSite simplified="1" location="1-25" boundStrand="0" annealedBases="TACCAATGCTTAATCAGTGAGGCAC" meltingTemperature="59"><Component hybridizedRange="1-25" bases="TACCAATGCTTAATCAGTGAGGCAC"/></BindingSite></Primer><Primer recentID="1" name="P573 Tn_Mar4" sequence="ATAATTGCTCGAGGGCGCGCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2484-2493" boundStrand="0" annealedBases="AGGGCGCGCC" meltingTemperature="54"><Component bases="ATAATTGCTCG"/><Component hybridizedRange="2484-2493" bases="AGGGCGCGCC"/></BindingSite><BindingSite location="1134-1154" boundStrand="1" annealedBases="ATAATTGCTCGAGGGCGCGCC" meltingTemperature="65"><Component hybridizedRange="1134-1154" bases="ATAATTGCTCGAGGGCGCGCC"/></BindingSite><BindingSite simplified="1" location="2484-2493" boundStrand="0" annealedBases="AGGGCGCGCC" meltingTemperature="54"><Component bases="ATAATTGCTCG"/><Component hybridizedRange="2484-2493" bases="AGGGCGCGCC"/></BindingSite><BindingSite simplified="1" location="1134-1154" boundStrand="1" annealedBases="ATAATTGCTCGAGGGCGCGCC" meltingTemperature="65"><Component hybridizedRange="1134-1154" bases="ATAATTGCTCGAGGGCGCGCC"/></BindingSite></Primer><Primer recentID="2" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1232-1258" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="1232-1258" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="1232-1258" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="1232-1258" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="3" name="KROL375  R6KG_ori_NspI_F" sequence="atatacatgtTGTCAGCCGTTAAGTGTTCCTGTG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-11-30T19:28:46Z"><BindingSite location="3848-3871" boundStrand="0" annealedBases="TGTCAGCCGTTAAGTGTTCCTGTG" meltingTemperature="62"><Component bases="atatacatgt"/><Component hybridizedRange="3848-3871" bases="TGTCAGCCGTTAAGTGTTCCTGTG"/></BindingSite><BindingSite simplified="1" location="3848-3871" boundStrand="0" annealedBases="TGTCAGCCGTTAAGTGTTCCTGTG" meltingTemperature="62"><Component bases="atatacatgt"/><Component hybridizedRange="3848-3871" bases="TGTCAGCCGTTAAGTGTTCCTGTG"/></BindingSite></Primer><Primer recentID="4" name="KROL376  R6KG_ori_NspI_R" sequence="atatacatgtGATCTGAAGATCAGCAGTTCAACCT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-11-30T19:30:47Z"><BindingSite location="4212-4236" boundStrand="1" annealedBases="GATCTGAAGATCAGCAGTTCAACCT" meltingTemperature="59"><Component bases="atatacatgt"/><Component hybridizedRange="4212-4236" bases="GATCTGAAGATCAGCAGTTCAACCT"/></BindingSite><BindingSite simplified="1" location="4212-4236" boundStrand="1" annealedBases="GATCTGAAGATCAGCAGTTCAACCT" meltingTemperature="59"><Component bases="atatacatgt"/><Component hybridizedRange="4212-4236" bases="GATCTGAAGATCAGCAGTTCAACCT"/></BindingSite></Primer><Primer recentID="5" name="P572 Tn_Mar3" sequence="attaagtttatttttcaaagcttgaagg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1167-1194" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="1167-1194" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite><BindingSite simplified="1" location="1167-1194" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="1167-1194" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite></Primer><Primer recentID="6" name="P571 Tn_Mar2" sequence="TTGGATAATTGCTCGAGGGCGCGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1135-1158" boundStrand="1" annealedBases="TTGGATAATTGCTCGAGGGCGCGC" meltingTemperature="66"><Component hybridizedRange="1135-1158" bases="TTGGATAATTGCTCGAGGGCGCGC"/></BindingSite><BindingSite simplified="1" location="1135-1158" boundStrand="1" annealedBases="TTGGATAATTGCTCGAGGGCGCGC" meltingTemperature="66"><Component hybridizedRange="1135-1158" bases="TTGGATAATTGCTCGAGGGCGCGC"/></BindingSite></Primer><Primer recentID="7" name="KROL516 Tn_lnSeqA_R&#10;" sequence="atatttaattaaaaaaactagctaaataacatatat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2022-05-23T19:08:37Z"><BindingSite location="1199-1231" boundStrand="1" annealedBases="tttaattaaaaaaactagctaaataacatatat" meltingTemperature="51"><Component bases="ata"/><Component hybridizedRange="1199-1231" bases="tttaattaaaaaaactagctaaataacatatat"/></BindingSite><BindingSite simplified="1" location="1199-1231" boundStrand="1" annealedBases="tttaattaaaaaaactagctaaataacatatat" meltingTemperature="51"><Component bases="ata"/><Component hybridizedRange="1199-1231" bases="tttaattaaaaaaactagctaaataacatatat"/></BindingSite></Primer><Primer recentID="8" name="KROL517 Tn_lnSeqA_F" sequence="ATATGGTACCTAACAGGTTGGATGATAAG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2022-05-23T19:16:50Z"><BindingSite location="1071-1095" boundStrand="0" annealedBases="GGTACCTAACAGGTTGGATGATAAG" meltingTemperature="57"><Component bases="ATAT"/><Component hybridizedRange="1071-1095" bases="GGTACCTAACAGGTTGGATGATAAG"/></BindingSite><BindingSite location="2524-2544" boundStrand="1" annealedBases="CCTAACAGGTTGGATGATAAG" meltingTemperature="55"><Component bases="ATATGGTA"/><Component hybridizedRange="2524-2544" bases="CCTAACAGGTTGGATGATAAG"/></BindingSite><BindingSite simplified="1" location="1071-1095" boundStrand="0" annealedBases="GGTACCTAACAGGTTGGATGATAAG" meltingTemperature="57"><Component bases="ATAT"/><Component hybridizedRange="1071-1095" bases="GGTACCTAACAGGTTGGATGATAAG"/></BindingSite><BindingSite simplified="1" location="2524-2544" boundStrand="1" annealedBases="CCTAACAGGTTGGATGATAAG" meltingTemperature="55"><Component bases="ATATGGTA"/><Component hybridizedRange="2524-2544" bases="CCTAACAGGTTGGATGATAAG"/></BindingSite></Primer></Primers>
  c<Notes>
<UUID>4bfc4e6e-1213-4fb3-8bc3-ce62b2b16eb8</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="17:28:35">2022.7.8</Created>
<LastModified UTC="17:28:35">2022.7.8</LastModified>
<CreatedBy>kramseylab</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         6+ Cutters                                                                                                   d     CHO  d              E              Y? P                                                                                                                                                              )<?xml version="1.0"?><CustomEnzymeSets/>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
