	   SnapGene      tatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacaatcgatcaagggttggtttgcgcattcacagttctccgcaagaattgattggctccaattcttggagtggtgaatccgttagcgaggtgccgccggcttccattcaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgatctatacccttcaagctttgaaaaataaacttaattattatatatgttatttagctagtttttttaattaaagttaaaatcgagagcttgtttgacaaaaaaacaaaaaaatttcttgaaaattttttttttgactcaatatctagacttgcaagagcttggaactttgagattgttctaagatgcatacaaattcaaaatgcttaaacaaaaataatttaacaaaggagtaagattgttatgagccatattcaacgggaaacgtcttgctcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggaaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataaacttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagaattggttaattggttgtaacactggcagagcattacgctgacttgacgggacggcggctttgttgaataaatcgaacttttgctgagttgaaggatcagatcacgcatcttcccgacaacgcagaccgttccgtggcaaagcaaaagttcaaaatcaccaactggtccacctacaacaaagctctcatcaacgatccagctgtcccaaacgtccaggccttttgcagaagagatatttttaattgtggacgaatcgaactcaggaacttgatatttttcatttttttgctgttcagggatttgcagcatatcatggcgtgtaatatgggaaatgccgtatgtttccttatatggcttttggttcgtttctttcgcaaacgcttgagttgcgcctcctgccagcagtgcggtagtaaaggttaatactgttgcttgttttgcaaactttttgatgttcatcgttcatgtctccttttttatgtactgtgttagcggtctgcttcttccagccctcctgtttgaagatggcaagttagttacgcacaataaaaaaagacctaaaatatgtaaggggtgacgccaaagtatacactttgccctttacacattttaggtcttgcctgctttatcagtaacaaacccgcgcgatttacttttcgacctcattctattagactctcgtttggattgcaactggtctattttcctcttttgtttgatagaaaatcataaaaggatttgcagactacgggcctaaagaactaaaaaatctatctgtttcttttcattctctgtattttttatagtttctgttgcatgggcataaagttgcctttttaatcacaattcagaaaatatcataatatctcatttcactaaataatagtgaacggcaggtatatgtgatgggttaaaaaggatcgatcctctagctagagtcgatcttcgccagcagggcgaggatcgtggcatcaccgaaccgcgccgtgcgcgggtcgtcggtgagccagagtttcagcaggccgcccaggcggcccaggtcgccattgatgcgggccagctcgcggacgtgctcatagtccacgacgcccgtgattttgtagccctggccgacggccagcaggtaggccgacaggctcatgccggctgccgccgccttttcctcaatcgctcttcgttcgtctggaaggcagtacaccttgataggtgggctgcccttcctggttggcttggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccagcctcgcagagcaggattcccgttgagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaaggatggatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaagcgctgcttccctgctgttttgtggaatatctaccgactggaaacaggcaaatgcaggaaattactgaactgaggggacaggcgagagacgatgccaaagagctacaccgacgagctggccgagtgggttgaatcccgcgcggccaagaagcgccggcgtgatgaggctgcggttgcgttcctggcggtgagggcggatgtcgatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgcatatttgaatgtatttagaaaaataaacaaaagagtttgtagaaacgcaaaaaggccatccgtcaggatggccttctgcttaatttgatgcctggcagtttatggcgggcgtcctgcccgccaccctccgggccgttgcttcgcaacgttcaaatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgccgccaggcaaattctgttttatcagaccgcttctgcgttctgatttaatctgtatcaggctgaaaatcttctctcatccgccaaaacagccaagctcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgccaagcttgcatgcctgcaggtcgactctagaggatccccgggtacCtgcccaacgaaatatcatgaaaactggaacttacatcctagagtatatattgacttaaaagataaaaaaactaatagttgtccttactgtggaacgacttttaaagtagaaaaagaatccGCggccgcatatactgatattgaaatgaatagattaggtaaataagaattcgtaatcatgtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgacccgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaac  *  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                            %                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
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  !j<?xml version="1.0"?><Features nextValidID="27"><Feature recentID="2" name="peroxidase/catalase" directionality="1" translationMW="14936.01" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="596" hitsStopCodon="1"><Segment range="4375-4776" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="FTL_1504" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4375-4776" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4375-4776" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="26" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5238-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="25" name="P227 SP_pKL02_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5002-5025" color="#a020f0" type="standard"/><Q name="label"><V text="P227 SP_pKL02_R"/></Q><Q name="note"><V text="P227 SP_pKL02_R"/></Q></Feature><Feature recentID="24" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4864-4883" color="#a020f0" type="standard"/><Q name="label"><V text="P022 pEX18SeqR1"/></Q><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="23" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4842-4867" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="22" name="P226 SP_pKL02_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4793-4822" color="#a020f0" type="standard"/><Q name="label"><V text="P226 SP_pKL02_F"/></Q><Q name="note"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="21" name="P035 pEX18integrationVSVG" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4790-4818" color="#a020f0" type="standard"/><Q name="label"><V text="P035 pEX18integrationVSVG"/></Q><Q name="note"><V text="P035 pEX18integrationVSVG"/></Q></Feature><Feature recentID="20" name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4786-4821" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature recentID="19" name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4779-4821" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature recentID="18" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4290-4309" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="17" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4266-4289" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="16" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3367-3391" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="15" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="14" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="label"><V text="sacB"/></Q><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="13" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2007-2030" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P441 pEX_ckR"/></Q></Feature><Feature recentID="12" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="label"><V text="P027 pEX18KanSeq3F"/></Q><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1531-1553" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P443 pEX_kanR_R"/></Q></Feature><Feature recentID="10" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1259-1283" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P442 pEX_kanR_F"/></Q></Feature><Feature recentID="9" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="8" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="7" name="P049 groES promoter F" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="859-883" color="#a6acb3" type="standard"/><Q name="label"><V text="P049 groES promoter F"/></Q><Q name="note"><V text="P049 groES promoter F"/></Q></Feature><Feature recentID="6" name="P261 groESpCheck1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="826-859" color="#a020f0" type="standard"/><Q name="label"><V text="P261 groESpCheck1"/></Q><Q name="note"><V text="P261 groESpCheck1"/></Q></Feature><Feature recentID="5" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="4" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="3" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="label"><V text="P025 pEX18KanSeq1F"/></Q><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="5,4,3,2" nextValidID="6"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL7 Seq_pEX_2" sequence="tgtggaattgtgagcggata" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4863-4882" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="55"><Component hybridizedRange="4863-4882" bases="tgtggaattgtgagcggata"/></BindingSite><BindingSite simplified="1" location="4863-4882" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="55"><Component hybridizedRange="4863-4882" bases="tgtggaattgtgagcggata"/></BindingSite></Primer><Primer recentID="1" name="KROL6 Seq_pEX_1" sequence="gttttcccagtcacgacgtt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite><BindingSite simplified="1" location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite></Primer></Primers>
  h<Notes>
<UUID>defe9a53-d564-476c-b62f-c2d6273d5d17</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="19:12:30">2019.4.9</Created>
<LastModified UTC="14:14:25">2019.5.23</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  *     &          .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;Ӌr>DfZ ql^^jM`Ȗ`&*f+*\J3M#	%Bxꡊ+b益'㮽?*`Ԥ7YUQVbPs&wv㩴W1kG*x.jꊆE[ePj4yQyUnn*궻J`6TꥷZVmnڷH\4&b
UAK163ODM?Cг<pА<+.'~B*Oo:?OODk=ŔHQAw?Ko#P`f|U[9MS8⨁fǁ]XVGD|q8<H
=E3!8@"7F2PEYm٨XUD\P`㥨cҦ?=<+V2W]WǄcҿy9*޸J`B=Z⩴|댮mnt;=8xl#sӃ 5iJ\
rW=#бD	6ghVFZmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ             -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="0,1,2" nextValidID="27"><Feature recentID="26" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4836-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="25" name="P227 SP_pKL02_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4600-4623" color="#a020f0" type="standard"/><Q name="label"><V text="P227 SP_pKL02_R"/></Q><Q name="note"><V text="P227 SP_pKL02_R"/></Q></Feature><Feature recentID="24" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4462-4481" color="#a020f0" type="standard"/><Q name="label"><V text="P022 pEX18SeqR1"/></Q><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="23" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4440-4465" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="22" name="P226 SP_pKL02_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4391-4420" color="#a020f0" type="standard"/><Q name="label"><V text="P226 SP_pKL02_F"/></Q><Q name="note"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="21" name="P035 pEX18integrationVSVG" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4388-4416" color="#a020f0" type="standard"/><Q name="label"><V text="P035 pEX18integrationVSVG"/></Q><Q name="note"><V text="P035 pEX18integrationVSVG"/></Q></Feature><Feature recentID="20" name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4384-4419" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature recentID="19" name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4377-4419" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature recentID="18" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4290-4309" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="17" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4266-4289" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="16" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3367-3391" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="15" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="14" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="label"><V text="sacB"/></Q><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="13" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2007-2030" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P441 pEX_ckR"/></Q></Feature><Feature recentID="12" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="label"><V text="P027 pEX18KanSeq3F"/></Q><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1531-1553" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P443 pEX_kanR_R"/></Q></Feature><Feature recentID="10" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1259-1283" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P442 pEX_kanR_F"/></Q></Feature><Feature recentID="9" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="8" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="7" name="P049 groES promoter F" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="859-883" color="#a6acb3" type="standard"/><Q name="label"><V text="P049 groES promoter F"/></Q><Q name="note"><V text="P049 groES promoter F"/></Q></Feature><Feature recentID="6" name="P261 groESpCheck1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="826-859" color="#a020f0" type="standard"/><Q name="label"><V text="P261 groESpCheck1"/></Q><Q name="note"><V text="P261 groESpCheck1"/></Q></Feature><Feature recentID="5" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="4" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="3" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="label"><V text="P025 pEX18KanSeq1F"/></Q><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="5,4,3,2" nextValidID="6"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL7 Seq_pEX_2" sequence="tgtggaattgtgagcggata" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4461-4480" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="55"><Component hybridizedRange="4461-4480" bases="tgtggaattgtgagcggata"/></BindingSite><BindingSite simplified="1" location="4461-4480" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="55"><Component hybridizedRange="4461-4480" bases="tgtggaattgtgagcggata"/></BindingSite></Primer><Primer recentID="1" name="KROL6 Seq_pEX_1" sequence="gttttcccagtcacgacgtt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite><BindingSite simplified="1" location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite></Primer></Primers>
  h<Notes>
<UUID>defe9a53-d564-476c-b62f-c2d6273d5d17</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="19:12:30">2019.4.9</Created>
<LastModified UTC="14:14:15">2019.5.23</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  8     y  ;        ;.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;Ӌr>DfZ ql^^jM`Ȗ`&*f+*\J3M#	%Bxꡊ+b益'㮽?*`Ԥ7YUQVbPs&wv㩴W1kG*x.jꊆE[ePj4yQyUnn*궻J`6TꥷZVmnڷH\4&b
UAK163ODM?Cг<pА<+.'~B*Oo:?OODk=ŔHQAw?Ko#P`f|U[9MS8⨁fǁ]XVGD|q8<H
=E3!8@"7F2PEYm٨XUD\P`㥨cҦ?=<+V2W]WǄcҿy9*޸J`B=Z⩴|댮mnt;=8xl#sӃ 5iJ\
rW=#бD	jbbF<ZYQuvcz.u{Sũ~^*unM^
f+W9Bku/uhe[k<zL<ٞXYBVQk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>          @  C  :   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  !<?xml version="1.0"?><Features nextValidID="27"><Feature recentID="26" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5135-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="25" name="P227 SP_pKL02_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4899-4922" color="#a020f0" type="standard"/><Q name="label"><V text="P227 SP_pKL02_R"/></Q><Q name="note"><V text="P227 SP_pKL02_R"/></Q></Feature><Feature recentID="24" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4761-4780" color="#a020f0" type="standard"/><Q name="label"><V text="P022 pEX18SeqR1"/></Q><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="23" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4739-4764" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="22" name="P226 SP_pKL02_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4690-4719" color="#a020f0" type="standard"/><Q name="label"><V text="P226 SP_pKL02_F"/></Q><Q name="note"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="21" name="P035 pEX18integrationVSVG" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4687-4715" color="#a020f0" type="standard"/><Q name="label"><V text="P035 pEX18integrationVSVG"/></Q><Q name="note"><V text="P035 pEX18integrationVSVG"/></Q></Feature><Feature recentID="20" name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4683-4718" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature recentID="19" name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4676-4718" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature recentID="18" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4290-4309" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="17" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4266-4289" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="16" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3367-3391" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="15" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="14" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="label"><V text="sacB"/></Q><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="13" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2007-2030" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P441 pEX_ckR"/></Q></Feature><Feature recentID="12" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="label"><V text="P027 pEX18KanSeq3F"/></Q><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1531-1553" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P443 pEX_kanR_R"/></Q></Feature><Feature recentID="10" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1259-1283" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P442 pEX_kanR_F"/></Q></Feature><Feature recentID="9" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="8" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="7" name="P049 groES promoter F" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="859-883" color="#a6acb3" type="standard"/><Q name="label"><V text="P049 groES promoter F"/></Q><Q name="note"><V text="P049 groES promoter F"/></Q></Feature><Feature recentID="6" name="P261 groESpCheck1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="826-859" color="#a020f0" type="standard"/><Q name="label"><V text="P261 groESpCheck1"/></Q><Q name="note"><V text="P261 groESpCheck1"/></Q></Feature><Feature recentID="5" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="4" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="3" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="label"><V text="P025 pEX18KanSeq1F"/></Q><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="2" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4668-4673" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="hypothetical protein" directionality="1" translationMW="12027.67" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="168" maxRunOn="4718" maxFusedRunOn="4718"><Segment range="4375-4673" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="FTL_1548" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4375-4673" color="#00e600" type="standard"/></Feature></Features>
  *<?xml version="1.0"?><Primers nextValidID="6"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL7 Seq_pEX_2" sequence="tgtggaattgtgagcggata" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4760-4779" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="54"><Component hybridizedRange="4760-4779" bases="tgtggaattgtgagcggata"/></BindingSite><BindingSite simplified="1" location="4760-4779" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="54"><Component hybridizedRange="4760-4779" bases="tgtggaattgtgagcggata"/></BindingSite></Primer><Primer recentID="1" name="KROL6 Seq_pEX_1" sequence="gttttcccagtcacgacgtt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite><BindingSite simplified="1" location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite></Primer><Primer recentID="2" name="KROL158 FTL1548fragR" sequence="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4646-4684" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="4646-4684" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite><BindingSite simplified="1" location="4646-4684" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="4646-4684" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite></Primer><Primer recentID="3" name="KROL157 FTL1548fragF" sequence="atatGGTACCaaggcgatttttggatgtatgttaaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4368-4400" boundStrand="0" annealedBases="GGTACCaaggcgatttttggatgtatgttaaag" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="4368-4400" bases="GGTACCaaggcgatttttggatgtatgttaaag"/></BindingSite><BindingSite simplified="1" location="4368-4400" boundStrand="0" annealedBases="GGTACCaaggcgatttttggatgtatgttaaag" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="4368-4400" bases="GGTACCaaggcgatttttggatgtatgttaaag"/></BindingSite></Primer><Primer recentID="4" name="P813 CompFTL1548_1Ra" sequence="atatGGATCCttataaaggatcatcatcaaaagttaagc" description="Contains BamHI site; F. tularensis LVS"><BindingSite location="4647-4672" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="atatGGATCCtta"/><Component hybridizedRange="4647-4672" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="4647-4672" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="atatGGATCCtta"/><Component hybridizedRange="4647-4672" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer><Primer recentID="5" name="P813 CompFTL1548_1R" sequence="ttataaaggatcatcatcaaaagttaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4647-4672" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="tta"/><Component hybridizedRange="4647-4672" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="4647-4672" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="tta"/><Component hybridizedRange="4647-4672" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer></Primers>
  g<Notes>
<UUID>defe9a53-d564-476c-b62f-c2d6273d5d17</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="19:12:30">2019.4.9</Created>
<LastModified UTC="14:14:8">2019.5.23</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  +                .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;Ӌr>DfZ ql^^jM`Ȗ`&*f+*\J3M#	%Bxꡊ+b益'㮽?*`Ԥ7YUQVbPs&wv㩴W1kG*x.jꊆE[ePj4yQyUnn*궻J`6TꥷZVmnڷH\4&b
UAK163ODM?Cг<pА<+.'~B*Oo:?OODk=ŔHQAw?Ko#P`f|U[9MS8⨁fǁ]XVGD|q8<H
=E3!8@"7F2PEYm٨XUD\P`㥨cҦ?=<+V2W]WǄcҿy9*޸J`B=Z⩴|댮mnt;=8xl#sӃ 5iJ\
rW=#бD	ZaB%*-QI)%zUW%U&i`&멹-:Q#:WVZAQV+F}4'QTKNUUc!EfᩲQ[k[TQW;-j<ٞXYBVQk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>        !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #<?xml version="1.0"?><Features nextValidID="28"><Feature recentID="27" name="/dnas_title=pKL02 Verified" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-5635" color="#ffffff" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q><Q name="note"><V text="/dnas_title=pKL02 Verified"/></Q></Feature><Feature recentID="26" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q><Q name="label"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="25" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="24" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="23" name="P261 groESpCheck1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="826-859" color="#a020f0" type="standard"/><Q name="note"><V text="P261 groESpCheck1"/></Q><Q name="label"><V text="P261 groESpCheck1"/></Q></Feature><Feature recentID="22" name="P049 groES promoter F" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="859-883" color="#a6acb3" type="standard"/><Q name="note"><V text="P049 groES promoter F"/></Q><Q name="label"><V text="P049 groES promoter F"/></Q></Feature><Feature recentID="21" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="20" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q><Q name="label"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="19" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1259-1283" color="#a6acb3" type="standard"/><Q name="note"><V text="P442 pEX_kanR_F"/></Q><Q name="label"><V text="nonstandard type: PCR_primer"/></Q></Feature><Feature recentID="18" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1531-1553" color="#a6acb3" type="standard"/><Q name="note"><V text="P443 pEX_kanR_R"/></Q><Q name="label"><V text="nonstandard type: PCR_primer"/></Q></Feature><Feature recentID="17" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q><Q name="label"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="16" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2007-2030" color="#a6acb3" type="standard"/><Q name="note"><V text="P441 pEX_ckR"/></Q><Q name="label"><V text="nonstandard type: PCR_primer"/></Q></Feature><Feature recentID="15" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2119" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q><Q name="label"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="14" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q><Q name="label"><V text="sacB"/></Q></Feature><Feature recentID="13" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="12" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3367-3391" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="11" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4266-4289" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q><Q name="label"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="10" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4290-4309" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q><Q name="label"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="9" name="FTL_1743 fragment" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4375-4873" color="#993366" type="standard"/><Q name="note"><V text="FTL_1743 fragment"/></Q><Q name="label"><V text="FTL_1743 fragment"/></Q></Feature><Feature recentID="8" name="P002 FTL_1743fragF" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4375-4401" color="#a020f0" type="standard"/><Q name="note"><V text="P002 FTL_1743fragF"/></Q><Q name="label"><V text="P002 FTL_1743fragF"/></Q></Feature><Feature recentID="7" name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4848-4918" color="#a020f0" type="standard"/><Q name="note"><V text="P001 FTL_1743fragR"/></Q><Q name="label"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature recentID="6" name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4883-4918" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature recentID="5" name="P035 pEX18integrationVSVG" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4887-4915" color="#a020f0" type="standard"/><Q name="note"><V text="P035 pEX18integrationVSVG"/></Q><Q name="label"><V text="P035 pEX18integrationVSVG"/></Q></Feature><Feature recentID="4" name="P226 SP_pKL02_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4890-4919" color="#a020f0" type="standard"/><Q name="note"><V text="P226 SP_pKL02_F"/></Q><Q name="label"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="3" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4939-4964" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q><Q name="label"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="2" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4961-4980" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q><Q name="label"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="1" name="P227 SP_pKL02_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5099-5122" color="#a020f0" type="standard"/><Q name="note"><V text="P227 SP_pKL02_R"/></Q><Q name="label"><V text="P227 SP_pKL02_R"/></Q></Feature><Feature recentID="0" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5335-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q><Q name="label"><V text="ColE origin"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  q<Notes>
<UUID>ab50ccb3-a294-49b1-80d4-d64540352dc7</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Description>synthetic circular DNA</Description>
<Created UTC="4:0:0">2018.7.21</Created>
<LastModified UTC="19:11:25">2019.4.9</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
        {  A        Af	jbbF<ZYQuvcz.u{Sũ~^*unM^
f+W9Bku/uhe[k<zL<          
  4  =  @  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  ^<?xml version="1.0"?><Features nextValidID="3"><Feature recentID="2" name="FTL_1548" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11-309" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="hypothetical protein" directionality="1" translationMW="12027.67" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="168" maxRunOn="321" maxFusedRunOn="321"><Segment range="11-309" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="304-309" color="#a6acb3" type="standard"/></Feature></Features>
  
<?xml version="1.0"?><Primers nextValidID="4"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P813 CompFTL1548_1R" sequence="ttataaaggatcatcatcaaaagttaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="283-308" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="tta"/><Component hybridizedRange="283-308" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="283-308" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="tta"/><Component hybridizedRange="283-308" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer><Primer recentID="1" name="P813 CompFTL1548_1Ra" sequence="atatGGATCCttataaaggatcatcatcaaaagttaagc" description="Contains BamHI site; F. tularensis LVS"><BindingSite location="283-308" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="atatGGATCCtta"/><Component hybridizedRange="283-308" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="283-308" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="atatGGATCCtta"/><Component hybridizedRange="283-308" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer><Primer recentID="2" name="KROL157 FTL1548fragF" sequence="atatGGTACCaaggcgatttttggatgtatgttaaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-36" boundStrand="0" annealedBases="atatGGTACCaaggcgatttttggatgtatgttaaag" meltingTemperature="62"><Component hybridizedRange="0-36" bases="atatGGTACCaaggcgatttttggatgtatgttaaag"/></BindingSite><BindingSite simplified="1" location="0-36" boundStrand="0" annealedBases="atatGGTACCaaggcgatttttggatgtatgttaaag" meltingTemperature="62"><Component hybridizedRange="0-36" bases="atatGGTACCaaggcgatttttggatgtatgttaaag"/></BindingSite></Primer><Primer recentID="3" name="KROL158 FTL1548fragR" sequence="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="282-320" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="282-320" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite><BindingSite simplified="1" location="282-320" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="282-320" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite></Primer></Primers>
  g<Notes>
<UUID>395d1b84-d57b-4633-89b7-4c0e12448ebb</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="19:10:51">2019.4.9</Created>
<LastModified UTC="19:12:24">2019.4.9</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  
7zXZ  ִF !   t/{	] ˇf{3(1v%f M*	mj)p80*i	NY]QF7&!n]NDc[A)xS@x,YӋ>;oVfa♄ҖY_͗<Y2}bT)4C-|5.?eL8'e
C9V.Qj^  yvƤͨ{*es##i6`c	H,@кJ-fd?F}RJMTZZy"ΖeR-{tLbH) Q#rT%/Pq&fمG;u{a״wW"tnĦ0]،C gYݗ+>?x%	l|tS"Z;Bn2Gذg	}̃:W# =^VT%&/236mgE3mc!aAݷwRa3e{ HhW3Z9;_(,2ж\_iQNq>h_}Oc0}-e77rOt!ẃ8@>AZc8T{C̥kN-c`;2+Ur+K!8$`x>vݐtl޹)bzv ٓzFq+*띴]hސ|믣ҳrs䑌UGp\g%ZO25Cal/GPɉyyӱ)38C:8RXnYux?@7YEw8`7V! vGUMRq,ŕK \#ଜ\%mWGQ SʉpCU1kQ=lx%1BC@YOߓ;Xn`W\k +R5qMBa DU??SGjԳ]pަ̰(!H4o=HbLCA6v܅uCC}ǎŀl'jeJzsۈ$j.([W%{ڤcϊ<%n3M?`zFaqHlJkDF-Mchm e1ت-Җ/e;7U
	Ll$.+=1Fdk&?SGR_YT!3Rw^|tn%yVlԋpW*>cX]	3$ṣWaWF
AK _q:g*?p$l?a>Q	%8mQR1?dۂidmAǨ[QZ4Պ3.ThF4g`ϭ؃偶mLu/^EAڧTdJ)Q%}	1}8Kh¼9%{lIܙxw2^#+U[n)_-&e!8dU׸{:BD_Vle&C`$w(|S&x\M`
:~.4<`+6=ՍM뮟y;)k7,[d ]yȗsMJ{J+#hImJArm5N>[c*^v~*D5$Q[Ev8g=P)]3T+"8U]J0ZT\<g|?VU2:M4ew 8AEWNͮ%PWեC"# IRԓE!PHRcNT}D?9a)BgtZw{*J/z<t\K4rwԌ6_T `V˨5JX:*lRlK˝&?G2۝60jGet|ԓ|:B⣨`[r	vRjG()؋h{H'(DtZID>;X[;+6Iha!.]2c-]W1݄~(=WΚi1Ye8_jNG3moPDw[V8&㖁 j::A8[:hsG2|bEl{:}[@'5ۦ]Hasz X8ff1%`z؆[7Ol5=`gPN>T3ekQ04!NU`;8?:Ip>66\l)5*$sdDhv@w yV|s+=
$Ql("R)+;+:~p(N\	sVզlkl{gT})#s&BԎdgV:mKVfvBN$Lyfp[N?K"bqk ^k2
U     ىz/#  x1g    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  9<?xml version="1.0"?><Features nextValidID="39"><Feature recentID="0" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="label"><V text="P025 pEX18KanSeq1F"/></Q><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="1" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="2" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="3" name="P261 groESpCheck1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="826-859" color="#a020f0" type="standard"/><Q name="label"><V text="P261 groESpCheck1"/></Q><Q name="note"><V text="P261 groESpCheck1"/></Q></Feature><Feature recentID="4" name="P049 groES promoter F" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="859-883" color="#a6acb3" type="standard"/><Q name="label"><V text="P049 groES promoter F"/></Q><Q name="note"><V text="P049 groES promoter F"/></Q></Feature><Feature recentID="5" name="groES promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="6" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="7" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1259-1283" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P442 pEX_kanR_F"/></Q></Feature><Feature recentID="8" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1531-1553" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P443 pEX_kanR_R"/></Q></Feature><Feature recentID="9" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="label"><V text="P027 pEX18KanSeq3F"/></Q><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="10" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2007-2030" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P441 pEX_ckR"/></Q></Feature><Feature recentID="11" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="label"><V text="sacB"/></Q><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="12" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="13" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3367-3391" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="14" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4266-4289" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="15" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4290-4309" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="16" name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4497-4539" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature recentID="17" name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4504-4539" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature recentID="18" name="P035 pEX18integrationVSVG" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4508-4536" color="#a020f0" type="standard"/><Q name="label"><V text="P035 pEX18integrationVSVG"/></Q><Q name="note"><V text="P035 pEX18integrationVSVG"/></Q></Feature><Feature recentID="19" name="P226 SP_pKL02_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4511-4540" color="#a020f0" type="standard"/><Q name="label"><V text="P226 SP_pKL02_F"/></Q><Q name="note"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="20" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4560-4585" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="21" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4582-4601" color="#a020f0" type="standard"/><Q name="label"><V text="P022 pEX18SeqR1"/></Q><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="22" name="P227 SP_pKL02_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4720-4743" color="#a020f0" type="standard"/><Q name="label"><V text="P227 SP_pKL02_R"/></Q><Q name="note"><V text="P227 SP_pKL02_R"/></Q></Feature><Feature recentID="23" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4956-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="24" name="FTL_0143" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4494-4494" color="#00e600" type="standard"/></Feature><Feature recentID="25" name="hypothetical protein" directionality="2" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="127" maxRunOn="4458" maxFusedRunOn="4458"><Segment range="4494-4494" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V/></Q></Feature><Feature recentID="26" name="FTL_0144" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4375-4494" color="#00e600" type="standard"/></Feature><Feature recentID="27" name="hypothetical protein" directionality="1" translationMW="4774.44" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="18" maxRunOn="4539" maxFusedRunOn="4539"><Segment range="4375-4494" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="CPTKYHENWNLHPRVYIDLKDKKTNSCPYCGTTFKVEKES"/></Q></Feature><Feature recentID="28" name="KanR" directionality="1" translationMW="30979.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature recentID="29" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2384-2829" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3203-3312" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="31" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3874-3960" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="32" name="VSV-G tag" directionality="1" translationMW="1339.54" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4539" maxFusedRunOn="4539" detectionMode="exactProteinMatch"><Segment range="4504-4536" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;epitope tag from vesicular stomatitis virus G protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="YTDIEMNRLGK"/></Q></Feature><Feature recentID="33" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="4602-4608" color="#ffffff" type="standard"/><Segment range="4609-4626" color="#ffffff" type="standard"/><Segment name="-35" range="4627-4632" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3755-3782" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4647-4668" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="36" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4310-4326" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4554-4570" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4578-4594" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
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  h<Notes>
<UUID>f1216ba9-1a3d-456f-9577-44d59a681632</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:31:34">2021.10.4</Created>
<LastModified UTC="16:34:8">2021.10.4</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         6+ Cutters                                                                                                   d     CHO  d              E              Y? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
