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GACGGGTTGTTACTCGCTCACATTTAATGTTGATGAAAGCTGGCTACAGGAAGGCCAGACGCGAATTATTTTTGATGGCGTTAACTCGGCGTTTCATCTGTGGTGCAACGGGCGCTGGGTCGGTTACGGCCAGGACAGTCGTTTGCCGTCTGAATTTGACCTGAGCGCATTTTTACGCGCCGGAGAAAACCGCCTCGCGGTGATGGTGCTGCGCTGGAGTGACGGCAGTTATCTGGAAGATCAGGATATGTGGCGGATGAGCGGCATTTTCCGTGACGTCTCGTTGCTGCATAAACCGACTACACAAATCAGCGATTTCCATGTTGCCACTCGCTTTAATGATGATTTCAGCCGCGCTGTACTGGAGGCTGAAGTTCAGATGTGCGGCGAGTTGCGTGACTACCTACGGGTAACAGTTTCTTTATGGCAGGGTGAAACGCAGGTCGCCAGCGGCACCGCGCCTTTCGGCGGTGAAATTATCGATGAGCGTGGTGGTTATGCCGATCGCGTCACACTACGTCTGAACGTCGAAAACCCGAAACTGTGGAGCGCCGAAATCCCGAATCTCTATCGTGCGGTGGTTGAACTGCACACCGCCGACGGCACGCTGATTGAAGCAGAAGCCTGCGATGTCGGTTTCCGCGAGGTGCGGATTGAAAATGGTCTGCTGCTGCTGAACGGCAAGCCGTTGCTGATTCGAGGCGTTAACCGTCACGAGCATCATCCTCTGCATGGTCAGGTCATGGATGAGCAGACGATGGTGCAGGATATCCTGCTGATGAAGCAGAACAACTTTAACGCCGTGCGCTGTTCGCATTATCCGAACCATCCGCTGTGGTACACGCTGTGCGACCGCTACGGCCTGTATGTGGTGGATGAAGCCAATATTGAAACCCACGGCATGGTGCCAATGAATCGTCTGACCGATGATCCGCGCTGGCTACCGGCGATGAGCGAACGCGTAACGCGAATGGTGCAGCGCGATCGTAATCACCCGAGTGTGATCATCTGGTCGCTGGGGAATGAATCAGGCCACGGCGCTAATCACGACGCGCTGTATCGCTGGATCAAATCTGTCGATCCTTCCCGCCCGGTGCAGTATGAAGGCGGCGGAGCCGACACCACGGCCACCGATATTATTTGCCCGATGTACGCGCGCGTGGATGAAGACCAGCCCTTCCCGGCTGTGCCGAAATGGTCCATCAAAAAATGGCTTTCGCTACCTGGAGAGACGCGCCCGCTGATCCTTTGCGAATACGCCCACGCGATGGGTAACAGTCTTGGCGGTTTCGCTAAATACTGGCAGGCGTTTCGTCAGTATCCCCGTTTACAGGGCGGCTTCGTCTGGGACTGGGTGGATCAGTCGCTGATTAAATATGATGAAAACGGCAACCCGTGGTCGGCTTACGGCGGTGATTTTGGCGATACGCCGAACGATCGCCAGTTCTGTATGAACGGTCTGGTCTTTGCCGACCGCACGCCGCATCCAGCGCTGACGGAAGCAAAACACCAGCAGCAGTTTTTCCAGTTCCGTTTATCCGGGCAAACCATCGAAGTGACCAGCGAATACCTGTTCCGTCATAGCGATAACGAGCTCCTGCACTGGATGGTGGCGCTGGATGGTAAGCCGCTGGCAAGCGGTGAAGTGCCTCTGGATGTCGCTCCACAAGGTAAACAGTTGATTGAACTGCCTGAACTACCGCAGCCGGAGAGCGCCGGGCAACTCTGGCTCACAGTACGCGTAGTGCAACCGAACGCGACCGCATGGTCAGAAGCCGGGCACATCAGCGCCTGGCAGCAGTGGCGTCTGGCGGAAAACCTCAGTGTGACGCTCCCCGCCGCGTCCCACGCCATCCCGCATCTGACCACCAGCGAAATGGATTTTTGCATCGAGCTGGGTAATAAGCGTTGGCAATTTAACCGCCAGTCAGGCTTTCTTTCACAGATGTGGATTGGCGATAAAAAACAACTGCTGACGCCGCTGCGCGATCAGTTCACCCGTGCACCGCTGGATAACGACATTGGCGTAAGTGAAGCGACCCGCATTGACCCTAACGCCTGGGTCGAACGCTGGAAGGCGGCGGGCCATTACCAGGCCGAAGCAGCGTTGTTGCAGTGCACGGCAGATACACTTGCTGATGCGGTGCTGATTACGACCGCTCACGCGTGGCAGCATCAGGGGAAAACCTTATTTATCAGCCGGAAAACCTACCGGATTGATGGTAGTGGTCAAATGGCGATTACCGTTGATGTTGAAGTGGCGAGCGATACACCGCATCCGGCGCGGATTGGCCTGAACTGCCAGCTGGCGCAGGTAGCAGAGCGGGTAAACTGGCTCGGATTAGGGCCGCAAGAAAACTATCCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAACGGTCTGCGCTGCGGGACGCGCGAATTGAATTATGGCCCACACCAGTGGCGCGGCGACTTCCAGTTCAACATCAGCCGCTACAGTCAACAGCAACTGATGGAAACCAGCCATCGCCATCTGCTGCACGCGGAAGAAGGCACATGGCTGAATATCGACGGTTTCCATATGGGGATTGGTGGCGACGACTCCTGGAGCCCGTCAGTATCGGCGGAATTCCAGCTGAGCGCCGGTCGCTACCATTACCAGTTGGTCTGGTGTCAAAAATAAGgatccactagtgagctcatgcatgatatcgtccgggccgcaagctcctagcggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctcaagctagagagtcattaccccaggcgtttaagggcaccaataactgccttaaaaaaattacgccccgccctgccactcatcgcagtctagcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagctcagctaattaag  EG                  %   +      $            3   '       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   "      ,                  0   0   #   2   +       %      G            *   (   )                                    *      5                     !      J   4   /   %   +         (   '   '   "   4   ,            -            (   6            !   &             +   "            )            2   #            *   ,   <   #   .   <                   !   %   $   !   
               %   &   #         )   !                  *      '      ,   *   &   +   0   T   /             "            %   (             +                                  )      *   .                  ,   !   3   9   !   "   .   +      !      %                        %   B   7      +                     )                     +      7   '            (      $      $                 ]

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QY v*OtC'PwB UE}*tv   w		0
,Tu:~T }O			
2
d}#h&Y   ,b0	9	n	Mz[MU\j5 W  ,NwH:3_< - aK	<
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qwHT>v\?+ (]*	
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9C5*  DYi4H]qv>	y		O9|B^=\gQ\e6=; C Fsw
kQ|\5 -g	
V	6Wh}r/T'@?iaF<V#  1n6 		

#Q+"^;\}KVYs{X   }

%./]qn5bg  7 	{
v*\Hi $~
lBxe	u   +NMv/	8	m			/
y+"t[ B  Ev)		N8<f[>! 0z

"g|J[`4a  #M
(	.		
MkftJ ' (           i[\	-	
Wj"#%&=}<(#$%N
n4      	`Q=mBKab'>X~3uH &}&// )  Gj]	
X$=)!Owdco% 6 
2Rj/=b		


(Y8l''+    :C:7c?4zT~
    a>L4
3evIk  Y.bnbOSRj Kp0( AH 		n


:%.-:"z+2HMtp' * T   gkB^d	T


0
hPBUdg,V'  %;Y		`

	,Y5GHIqN(g["  9mG


k>*1xCe4 jz M3~#P>"


AZW9JUd9(bS(`7, 4F'7V5			'
K

aoQYy$m! 7 sk0c	
^f8  nISO!m2	8

))` [t(8@ A{-  % R 	W,;IY#8;E
R
K 1Kl  g  2;x!5DNBDFdn$ u  	
F]dO5UJ  (?U~	}&O Sk^(   ? ]z	FMs	5
4iHwIJ.Gl6 H n y   t4!J|/h(B"t| 	S_fl%xD .=bqdo?	w%  oPU	r

TMfF|m&  -;	
(^>LZq f3ES! i  Zcd7c_	
p*KP1U]sO& 9`/p		|ocDSkApJ;oS1  B
< LdpLyF *%
H	o



;&8aNq IJ	
.;s{+ 3		n&/#,3>W]48I&u|" + U l_e	
*
(U7i3OVhW    !C9`l


1 ;{+ae- # w hWS	
\adQC  ! Y UG	U

&B5nQL&) [ Z&+Z	W
<-HfIJ6h\J 3<	
rzp   PyV		a
e
[HZO7  ?SzW	

 >6z)M2  j:Iz


D+52	/DNC1N]# *W
H


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4EL8gcz*  kJ(\	>B3}U[EXV7Ha8L, N$Q?#		w(X:X[Ke:`#T!< 48R	#

sJDOCti)p-oxU)A`q -#  5Wu			L
K[bpRZ%@.  @8&	6	

wFxzn^<e*< l rDG%*3M-k6	P

Qu7#wXH6^"    (		(
j

y4>JD$m 8 l:d	{2gV	  

s 2 N   =flw	__]v9 : a tnCW1/hG! \"<fY
`i*@P-	%   JTAfTE	9

R#[Gh=@@y$ / ] OoPcf	3		>
LW= t!v! `n	A

"*a*j
 q )so|  |aG9 LT G\up{AB[=  ~ |5
$u!|z; A  *uLuyl	.
!d;eZn%  &   _
<lJZ,		LVi
2t|m& 5 1X-ai.9<6d	NRyJj# S 
$F
S


+Fjp	01gLs 3y"6O	7
`e_  Q wH'CE 0@E)  > <y'Er>Tg}'A!s{			|kG!  h  8O	n)JCTLon %R 2
;%KrV 7 v  8_R		AT6*4KN >R
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=Gey yLI 
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'2DoT_*     $?)Lt			
P
l5; M `   Vkv^\uF'  N_@		
Q~!KVZO) p {`FK~nS, 4 @  ^khtKtx-	5 UchcdiQ/m*   P v7GNS|&z	6
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71Q	
c
<:x)H$Jx/H+ L    #^Kju		
  JU[	N(E0 o u66JR_s{%y	z;JTbg9bm'c(lT I J K        5"][\}~i)#$X}~			{			


`a:;78IT`a&ab'y^_/  F]0*CBY|		<djz=-v`  Z}	k>&.w  />pG"1nD\N8?Z   e ^@+C		g
]max'=" c%r		(l=r@e_{ag9 [	s
e? E  RSpvyV	N~'}~ u
 
$1G'% Q~		
Ul|1*gc/xn( q2o[M<		1O_NrWg4FOe  ?.b	)P!$@ST 0|HY3
@]?S[E   Uhx#1E	

Zl6M19[+ j HdA8di	h
^yVvE^!+}t(> <   _pEe"qnL2P  L#[ D
8b-Qm&~ f,Y`		+ 
 c &:>m>Tl|hpT* ds	<b:2^ A0.		q	}jAfBqK8I a	)spdE`	 Ce\	qr#_,  lj
t

H%8e#lG[A $>i			=!M4Cs) [
,fo2Mt1@zV2>  S+I	
(9RD!*  @W		|
@8Fv)6R~Fr. F e [^Tq zp

'Odb"D`	1,H (w
<n-LOc5_ 
!Y2<~V> </	yw/H;X 	
 k%FY-j3X%  Jv<K"/	jt|4{(, Yh	027h-4B?^9J/Y0}! R4Kq	X$18PhPt13  	
b	o/}Bd}0EyA*EK9 			
j'
Vm,V -+9AX5 k'vo! uNT`&OjsvPX" V +		
2SZ^X}i4. , rCh`3p \=	

+
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8Pj[d4u5*<P{+ m~f	P).V*]p.?>UXhrmGWhf  /D	7*3`!   "
a<|"Mv%b.  a	
2Q	%  @f8:c		_
m



/4-,M9'BATZfU iL}4&
J


]ITR  $  1:VX"ZMs	D

A x 3pcI%^$HwRjF ,6T	
b0=@\:{eRDlr  $G	
mc7x\3{    " TVF

%'[`m2c% Z Yy2VU		V
Zo%  ViH	


C64NR:BM'\B 7I
'"	[	=rvICNK7BsDW_f6",n~GypK7 k qC2,j5	i

Jtw"vGC
#d])? 1  <eB9	
.gz1+ \ [ee	@
X
_h?
Fs ,iux    {4F#ro{:  	`
	Mos{ri = q=f r		_BQ 0@S3q2MK _     Ms		
1Yn 0E
b
.RP)  \QzW	
I96HSci<,M' $!	f
'\k[Mlq8 QR	
d	{0  Tg{0Z	a		
?Y0Nd "Xh!7,D* @-7 dk	
+n$"q"^U++  d '?	{
?Eu mc
Q,E^   ;;	x!X }3i=27 XJp	
.	U,6D07O)DgOJ0' t}		
-)o-=Tcl);gz3 e	^

63`L8_ 9+bC
I

/Gzkq  X
Y	lbw[$(  B;1	
	
BgsAr5	mxc zq?RnAq9J$ /	r	
j~k5GRXh;L !//C$ c		*t-P
C<f 	,K.FaZ#y pirm@gp bo	q,ef   zTTTN   {TTNT   |TNTA   }NTAG  TCAN  CANG  ANGC  NGCC  TGAN  GANT  ANTC  NTCA  &CGAN  'GANG  (ANGC  )NGCA  1  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                   &                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       $                                        	                                           !                                                                                                                   	            8      
               	      	                  	                         
         
                                          ,   7   0   
      2      %             u   \      %   m               ,   R                                                                                                                                                                        &                                                                                                                                                                                                                                                                                                                                                                                                       $              	               !                                         	    8  
     	  	      	         
   
              , 7 0 
  2  %     u \  % m     , R               OxM[x5G,-,;C[96N%V hWh	g	>>
hD	EEL
Lh(u59&q"Qbs
h$   D

6N{%>X	Z c3']Pc5q
7	xZ P G/Iu%u
2q	
[
  `5	MI	y%?\,8SD		"?SD	"?QcP4qSD	"`?oMG	7s XJ6ye
SMh{?ibk
>2D6s	p
dD	bc@bwxoj

,eg<x	p
dD	-L;SD		"`?3\"s6|q(Mnr,B2Ckoz2qbwh.\b]	n	nzM!_l:
tZ YV
c	TgF6j?Tf m#5	D6MPUXr
s u IS1Muh.Ru5SD	:"h.7?RY   AH0#3)s1V_6O2,8d&lf	.p.D)DmdQepmuQs	 Cy
	]
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name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="75" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4525-4584" color="#00e600" type="standard"/></Feature><Feature recentID="76" name="lipoprotein" directionality="1" translationMW="2089.68" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7665" maxFusedRunOn="7665"><Segment range="4525-4584" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="77" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4489-4489" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4488" 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  ,T<?xml version="1.0"?><Primers recycledIDs="0,19,20,21" nextValidID="23"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="2" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="3" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="4" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="5" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="6" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="7" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="8" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="9" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="10" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4627-4646" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4627-4646" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4627-4646" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4627-4646" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="11" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7639-7664" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7639-7664" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7639-7664" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7639-7664" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="12" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4830-4851" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4830-4851" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4830-4851" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4830-4851" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="13" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5352-5376" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5352-5376" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5352-5376" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5352-5376" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="14" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5878-5898" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5878-5898" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5878-5898" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5878-5898" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="15" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6400-6422" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6400-6422" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6400-6422" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6400-6422" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="16" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6924-6945" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6924-6945" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6924-6945" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6924-6945" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="17" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7454-7477" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7454-7477" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7454-7477" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7454-7477" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="18" name="KROL318  LacZ_construct_f" sequence="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4593-4614" boundStrand="0" annealedBases="GCCATGATTACGGATTCACTGG" meltingTemperature="58"><Component bases="gttctaattaagtagacaatgatagca"/><Component hybridizedRange="4593-4614" bases="GCCATGATTACGGATTCACTGG"/></BindingSite><BindingSite simplified="1" location="4593-4614" boundStrand="0" annealedBases="GCCATGATTACGGATTCACTGG" meltingTemperature="58"><Component bases="gttctaattaagtagacaatgatagca"/><Component hybridizedRange="4593-4614" bases="GCCATGATTACGGATTCACTGG"/></BindingSite></Primer><Primer recentID="22" name="KROL319  LacZ_construct_r" sequence="atatggatcCTTATTTTTGACACCAGACCAACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7640-7671" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7640-7671" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite><BindingSite simplified="1" location="7640-7671" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7640-7671" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite></Primer></Primers>
  b<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="17:44:34">2020.8.31</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
        &  W   	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     -4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>TH`Ćxve襅[%N&jY^8j)ơ&bjUdnfU.[4*LN#3iʧ\W2ץvNLQt4X1`Σ}<881Cxэc?nw!iʊݩb89Ps`^*ۈ#\,)t˅~6s<Wϳ`3!JnA(e|y[Lj(a<'1(yJ4W4,|ϫ!Zla2)2.,UW<V[0w78hSE>1
#X.8S8k_-o-`a4q48aM^#8o #9bShW=al|a|1̗1`3%"n8amp[qlm[&0<w=j?Q#`U91g?s	t*Vx420. m,fU_,:psJap.6wM*J5}H}1g⼶Le`8%<%tx^|<{I̒5s6E<L40UH;/ۇLV6%j_=-шeU׎ҷ7\v%!L6s,\P}(H4gz8c;s m Ujm<~|$Ճ|6Ӄ4$0%x;<U|x|hv&˯\.330ǯJm<K]5_OlP݃*`q?-&N<,}(.VPoy!;ca/N0{A+|uuѕqP.xO*>D2}eUZs=KkU\0uoFA/S           V   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Wg<?xml version="1.0"?><Features recycledIDs="3,4" nextValidID="78"><Feature recentID="5" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4593-7664" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="6" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4593-7664" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="7" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4610-4626" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="lacZ" directionality="1" translationMW="116321.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" hitsStopCodon="1"><Segment range="4593-7664" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4593-7664" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="10" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7187-7211" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6543-6567" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="12" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5661-5690" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="13" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4869-4892" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="14" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7660-7664" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="15" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7911-8005" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional 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site"/></Q></Feature><Feature recentID="19" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="20" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="21" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="22" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="23" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7666-8023" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="24" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="25" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3374-3374" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="26" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3372-3372" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="27" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3371" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="28" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3370-3370" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="29" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3369-3369" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="30" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3368-3368" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="31" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3366-3366" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="32" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3365-3365" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="33" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="34" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="35" name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="36" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2787-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="37" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2786-2786" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="38" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2784-2784" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="39" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2782-2782" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="40" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2780-2780" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2779-2779" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="42" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2778-2778" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="43" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2777-2777" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="44" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2776-2776" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="45" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="46" name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2258-2872" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="47" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="48" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="49" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="50" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="51" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="52" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="53" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="54" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="55" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="56" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="57" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="58" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="59" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="60" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="61" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="62" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="63" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="64" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1103-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="65" name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="309-1124" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="66" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-219" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="67" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-230" color="#a020f0" type="standard"/></Feature><Feature recentID="68" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="208-208" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="69" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="70" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="71" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="72" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="73" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="74" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4489-4584" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="75" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4525-4584" color="#00e600" type="standard"/></Feature><Feature recentID="76" name="lipoprotein" directionality="1" translationMW="2089.68" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4599" maxFusedRunOn="4599"><Segment range="4525-4584" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="77" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4489-4489" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4488" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="1" name="FTL_0420" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4444" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="hypothetical protein" directionality="1" translationMW="3690.28" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="90" hitsStopCodon="1"><Segment range="4339-4444" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
  ,T<?xml version="1.0"?><Primers recycledIDs="0,19,20,21" nextValidID="23"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="2" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="3" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="4" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="5" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="6" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="7" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="8" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="9" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="10" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4626-4645" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4626-4645" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4626-4645" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4626-4645" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="11" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7638-7663" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7638-7663" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7638-7663" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7638-7663" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="12" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4829-4850" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4829-4850" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4829-4850" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4829-4850" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="13" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5351-5375" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5351-5375" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5351-5375" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5351-5375" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="14" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5877-5897" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5877-5897" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5877-5897" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5877-5897" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="15" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6399-6421" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6399-6421" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6399-6421" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6399-6421" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="16" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6923-6944" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6923-6944" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6923-6944" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6923-6944" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="17" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7453-7476" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7453-7476" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7453-7476" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7453-7476" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="18" name="KROL318  LacZ_construct_f" sequence="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4592-4613" boundStrand="0" annealedBases="GCCATGATTACGGATTCACTGG" meltingTemperature="58"><Component bases="gttctaattaagtagacaatgatagca"/><Component hybridizedRange="4592-4613" bases="GCCATGATTACGGATTCACTGG"/></BindingSite><BindingSite simplified="1" location="4592-4613" boundStrand="0" annealedBases="GCCATGATTACGGATTCACTGG" meltingTemperature="58"><Component bases="gttctaattaagtagacaatgatagca"/><Component hybridizedRange="4592-4613" bases="GCCATGATTACGGATTCACTGG"/></BindingSite></Primer><Primer recentID="22" name="KROL319  LacZ_construct_r" sequence="atatggatcCTTATTTTTGACACCAGACCAACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7639-7670" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7639-7670" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite><BindingSite simplified="1" location="7639-7670" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7639-7670" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite></Primer></Primers>
  b<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="17:42:34">2020.8.31</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
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`I {RZ7]_$()(=qeOWM`;WAsS݊uw/]#fm@?݈1䳣i3|Oa:㒬pͶ×3\Ns@A[f9X]N18cIZ.zuG4ACqA,M%!昳,ޡT BV'MnmsIEG_#'1a7-ᒪ8Y#x"=u}1^̀R@{ymLpqhOwP3sIf6 !k ߊ[ &XmoqiH7"m~5ȚƇ}S>dkd*>_B1Y`/0]Z4ar D=>ξl]RRnUBG s!W/qDeޮB2F 1ے>Th%7B#P*RqVXjK/Ylw79X1)AH3  24  1g    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ^@<?xml version="1.0"?><Features nextValidID="76"><Feature recentID="0" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="1" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="2" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="3" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="4" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="5" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="208-208" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="6" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-230" color="#a020f0" type="standard"/></Feature><Feature recentID="7" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-219" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="8" name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="309-1124" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature recentID="9" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1103-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="10" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="11" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="12" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="13" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="14" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="15" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="16" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="17" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="18" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="19" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="20" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="21" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="22" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="23" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="24" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="25" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="26" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="27" name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2258-2872" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQL"/></Q></Feature><Feature recentID="28" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="29" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2776-2776" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="30" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2777-2777" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="31" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2778-2778" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="32" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2779-2779" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="33" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2780-2780" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="34" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2782-2782" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="35" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2784-2784" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="36" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2786-2786" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="37" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2787-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="38" name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"/></Q></Feature><Feature recentID="39" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="40" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3365-3365" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="42" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3366-3366" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="43" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3368-3368" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="44" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3369-3369" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="45" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3370-3370" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3371" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="47" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3372-3372" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="48" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3374-3374" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7693-8050" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="51" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="52" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7693-7721" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="56" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="57" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7744-7841" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="58" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7938-8032" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="59" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7687-7691" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="60" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4896-4919" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="61" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5688-5717" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="62" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6570-6594" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="63" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7214-7238" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="64" name="lacZ" directionality="1" translationMW="116321.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" hitsStopCodon="1"><Segment range="4620-7691" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="AMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK*"/></Q></Feature><Feature recentID="65" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4637-4653" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="66" name="FTL_1046" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4338-4340" color="#00e600" type="standard"/></Feature><Feature recentID="67" name="D-alanyl-D-alanine carboxypeptidase" directionality="2" translationMW="131.17" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4299" maxFusedRunOn="4299"><Segment range="4338-4340" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="L"/></Q></Feature><Feature recentID="68" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4596-4613" color="#00e600" type="standard"/></Feature><Feature recentID="69" name="30S ribosomal protein S21" directionality="1" translationMW="701.89" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7691" maxFusedRunOn="7691"><Segment range="4596-4613" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MPSVRI"/></Q></Feature><Feature recentID="70" name="TSS (BNdata)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4515-4515" color="#a6acb3" type="standard"/></Feature><Feature recentID="71" name="Potential -10 element" directionality="1" type="-10_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4504-4509" color="#a6acb3" type="standard"/></Feature><Feature recentID="72" name="extended -10" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4501-4502" color="#a6acb3" type="standard"/></Feature><Feature recentID="73" name="Potential -35" directionality="1" type="-35_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4482-4487" color="#a6acb3" type="standard"/></Feature><Feature recentID="74" name="rpsU2 5'UTR" directionality="1" type="5'UTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4515-4595" color="#a6acb3" type="standard"/></Feature><Feature recentID="75" name="Promoter rpsU2" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4338-4514" color="#ffffff" type="standard"/></Feature></Features>
  4<?xml version="1.0"?><Primers nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="1" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="3" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="4" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="5" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="6" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="7" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="8" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="9" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4653-4672" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4653-4672" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4653-4672" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4653-4672" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="10" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7665-7690" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7665-7690" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7665-7690" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7665-7690" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="11" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4856-4877" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4856-4877" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4856-4877" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4856-4877" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="12" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5378-5402" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5378-5402" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5378-5402" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5378-5402" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="13" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5904-5924" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5904-5924" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5904-5924" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5904-5924" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="14" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6426-6448" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6426-6448" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6426-6448" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6426-6448" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="15" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6950-6971" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6950-6971" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6950-6971" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6950-6971" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="16" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7480-7503" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7480-7503" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7480-7503" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7480-7503" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="17" name="KROL319  LacZ_construct_r" sequence="atatggatcCTTATTTTTGACACCAGACCAACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7666-7697" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7666-7697" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite><BindingSite simplified="1" location="7666-7697" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7666-7697" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite></Primer><Primer recentID="18" name="KROL472 PrpsU2_F" sequence="atatGGTACCaaaacctttaaccatataaatttctaaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2021-09-15T19:29:33Z"><BindingSite location="4332-4366" boundStrand="0" annealedBases="GGTACCaaaacctttaaccatataaatttctaaag" meltingTemperature="57"><Component bases="atat"/><Component hybridizedRange="4332-4366" bases="GGTACCaaaacctttaaccatataaatttctaaag"/></BindingSite><BindingSite simplified="1" location="4332-4366" boundStrand="0" annealedBases="GGTACCaaaacctttaaccatataaatttctaaag" meltingTemperature="57"><Component bases="atat"/><Component hybridizedRange="4332-4366" bases="GGTACCaaaacctttaaccatataaatttctaaag"/></BindingSite></Primer><Primer recentID="19" name="KROL473 5UTR_rpsU2_R" sequence="atatGCGGCCGCaattctaacgcttggcataccctct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2021-09-15T19:29:33Z"><BindingSite location="4588-4620" boundStrand="1" annealedBases="GCGGCCGCaattctaacgcttggcataccctct" meltingTemperature="71"><Component bases="atat"/><Component hybridizedRange="4588-4620" bases="GCGGCCGCaattctaacgcttggcataccctct"/></BindingSite><BindingSite simplified="1" location="4588-4620" boundStrand="1" annealedBases="GCGGCCGCaattctaacgcttggcataccctct" meltingTemperature="71"><Component bases="atat"/><Component hybridizedRange="4588-4620" bases="GCGGCCGCaattctaacgcttggcataccctct"/></BindingSite></Primer><Primer recentID="20" name="KROL399  lacZ_qPCR_F3" sequence="CCAACGTGACCTATCCCATTAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2023-01-06T14:43:20Z"><BindingSite location="4920-4941" boundStrand="0" annealedBases="CCAACGTGACCTATCCCATTAC" meltingTemperature="56"><Component hybridizedRange="4920-4941" bases="CCAACGTGACCTATCCCATTAC"/></BindingSite><BindingSite simplified="1" location="4920-4941" boundStrand="0" annealedBases="CCAACGTGACCTATCCCATTAC" meltingTemperature="56"><Component hybridizedRange="4920-4941" bases="CCAACGTGACCTATCCCATTAC"/></BindingSite></Primer><Primer recentID="21" name="KROL400 lacZ_qPCR_R3" sequence="TTCCTGTAGCCAGCTTTCATC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2023-01-06T14:44:28Z"><BindingSite location="5006-5026" boundStrand="1" annealedBases="TTCCTGTAGCCAGCTTTCATC" meltingTemperature="57"><Component hybridizedRange="5006-5026" bases="TTCCTGTAGCCAGCTTTCATC"/></BindingSite><BindingSite simplified="1" location="5006-5026" boundStrand="1" annealedBases="TTCCTGTAGCCAGCTTTCATC" meltingTemperature="57"><Component hybridizedRange="5006-5026" bases="TTCCTGTAGCCAGCTTTCATC"/></BindingSite></Primer></Primers>
  h<Notes>
<UUID>538fbe38-7f72-4a55-a86a-a0ebe0c31456</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="19:35:57">2021.9.15</Created>
<LastModified UTC="14:44:28">2023.1.6</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         6+ Cutters                                                                                                   d     CHO  d              E              Y? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
