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XD
f &)=ez7bl:DDYoHY_d8j|  !:!!"1#  Qx}b/mY   }y&aW<!]"  	2	1`v*}_| " Y-	]^&B@L  !"#5#  F QV [g B"?#	 T *!!!"  MwC !S"o""# h
zk O8`r !_"#  	7.*5>f"# u	"### M
%_"1&SK!""E"~# ' q>v	
Ecn{:(	JY^?!!" = I n`		gI?"# <|&	Fgo !!("  e	aT  H!T#   q&
_w<`Zy :# 9V  ""    p "  _ d H	5
N
8cO|ch'!!|"x## D	>XX1 y!>"q#    n> UmwNzl!9""""\#u## @   @L[vdE	k
9F3Qk3x#W"i"t"""# !e		
$a<ej,{  "!)! X V	*
QuF^
!!#' 4  FIRj{n?]	


e5} !!!!%""z##(  0')>?	P


[ob6:AYdc  [!!G   #	`JhSe4	D			T
3nq?R^q}ZE,$b    0!&  f  aai7
MQslz0Yxa+ a Ug	X	b	2
o

Uwuj2.<d!"  >A~M}I~ygq\ ,# G	FSh% r!# , } "8L!l*	

{Tmx-  x<	iekj# ; _R<"~"   tc[	Alb!!*# y  H\	
#,k%)~ V!  C: S!i!""' 7 L Q9y}*	-Yq*AEY!"### F}0rpH_'!98P !""C#j#  ]v<e
/UV*Pr6!c!!"I# J'y	t 
3^@!0]@Rx'.   }!!!7# [+;j	!(2A >!! (=mB~	
r

T+xCS!6  $4xF/		!
`

*t%+_A/{ss{."" K

X#KN  o!"""'   {-J		
p6`6pL9)}B}   ""3 B^	T			z

xg[X'E>"DI=`Ak1j !^   47YM[4|	E
#*f8Ocm;EtBZa0Ze4m25	Q8.CgJmZ N  !"##"#2#_#6  -XCB	
'>{`"oU@pz5+:*  !;!X!@""  $	=Dx3nw!"s# w { `	0 Z !(#&  	2C
PRy~]k9nXc!a!{!"
  |_
Z  ~v=_ !!^" & H 	
z'X]RJ!"D"}## ^ G!S#   Cb==WW k![#p#w#  Uc	E]w+ !!!""#   _(gh	3	C		
Z2XaD@`- a !/!  !FK 
ESLljx~ [(}  R!U!;"" u8x		)Oq &!""#6#B# .	'n!"!"""  ,Z	y
ws&_oyA|9M i  ##!#^#  	j^<Cv!"  G u\Q C"   '
YRWC! +7w		r(p
%8h A#
   'BtP @#  /  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        	                                    	                                                                      	                                                                                                          
                                                                                 
      "                                                                                                                                                                                     D         !                        H   ^   <         O                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  	            	                            	                                        
                               
  "                                                             D   !        H ^ <   O        '  Q    O  "_, K'y
G!^"##	
l!4T
WOJo ["[7Vn"VVn"V 9y
 W  KGxJ
m97 "i#4rI	

rI	

drI	

II\
~4#'.r (@5Va( 5Xdj##4%9E\@^G  hYI?K	E2 Bj9  B8!Wp# X# Rd*h3hTM Ml#.!XB!TQ+#yh!!4- h 94"!Rdy4
<a~"D<@Z";`
t*0c!""  7"[  B4#8!["#*Y
	B "!?k
_R 7c
t@")	!]"`r
x^!|=?!#y4
"h![ +#
@	y2##6 S Px%	O
j
n@@ "#r#@+#D[,== I   kjW 	H%kkhDJi o# 6 Q""y*!YXB
!FI!' !GS 1*JG	mdS1\(I^	 !F t5Xc*1[|X[   Z!!B"	-t!m"4 5 !304 	B !"}H#j9E\i
1DUs,m=   \(>J  .6!K			Kv74PohY! 8cw""#wl
g3 Hm>
?d| !!!y##45gG%6u!Uz%FO]aD G%3 Hm>
d|!y##? !!j>
X0J"LKZ6F!3 Hm>
?<-Vd)| !!!e" #v#y## 8cuw!""#i#} "K 	,)!U	E]!!x/mUF "]U\P.U]yjN5!#R#FB^

[x "X *

!!#H%~ U " S ( 	
._!E##9n"E/y;~&G^W7B   !n"",oy9L!F"{	Yx0O[""".>d.

M!mRt!!!2Gy}#TG #
&=z_ :!#
!!( 	._E#<-V)e" #v#X H*

%~ !!#	Ivz+* Q#+    p "  Cb==WW k![#p#w#S  gL-7)q !R"e"" ## COk:FO5 u   2YykAz+|K2@H	^	h		


r!n.	
 e'Q !,""h#  ^ G7Ng0 &!!8"{"#[+;j	!(2A >!Pi\9EV\at(	->@n> J     $ Se5h+6P!### ~v=_ !!^" >A~M}I~ygq\ ,#$ 5 u     2YykAIz+Se|"5K{2@A	H	^	h			
A




WNr!n.B	h
>[ 3We'7+Q6?C] !E!P!!!!!,"""###h#{#     %'KMOGIZ\moQG			
@i>ouFZASs(~Mh-}K%0IV~FbCFlnty|gq   \ r!/""",#p`SYn 2 >l(	i

.X"1`!{ !  u~U8c	x	E	)Oq]v &w+=_  !!!!!!^"""""#6#B#7 @ L    @L[v~Q9dy}*E		k
9F3v-Qk3xYq*#=AEY_ !!!W"^"i"t""""##g   EfiAb<4iU	)I+V4IN ZNmc$/4  	j^<Cv!"PR,-?qq;E"#. i Cdhnl%:[t7lK			{
hyKiYE#h6Kj!6Ba (p$ 2 7 k 5 A u    2YyAtMkApz+|-K2@	H	^	h		
x





@G:r!ni%k.N	;`
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OT`nQl6QsB΀uÎ<4?
|~/	.yFt^BNhS+y*Jx*ޡJ*F[[8!FoF(`(]hxFhR]`	`*xZDTB8-J}b;ӚEzgqx]Nf.cؽ6?11mX< "9(9hD0ŀ:&;6Ϯlq+TIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLAL     F  H     )  )       7zXZ  ִF !   t/] 3D].?I*ә_.ZvќP,Vj,ު~mC<ґ?vd;!U''yzYl.vU[Qec <L3cGq~=X?r+<ɵؿĊG]1|=ty?izd@Ppp}ݽ<,c^D2)=0m(w+}[)0|cK7tDi|6?i6J$MEԎ.j7ȡdw}fHAKnm/<`!H|r,7\LD'j ?.|'J&29?J EՋ%1DiFu?\mo]\`[SsGrnڦ"$X=i`hzЉwA<J$"%N1]I11Ҁz_yjs(J:tI
x`Z|rC N4NS!
ibl">,Ѣ?=	GԵZ`k`jǨ9.p*G	X+{	i%Y%NX%/W?S/;Wy;)OEqe 553O$lPqw#<G7.Ml3+&!%2Gs?Ř)
M2	q:Ps/sa*,2ko8<\,_KjA^ZmYq&_R|W08|Ft`[3r	sd5sNr0 ֆp"׎p DL܀d_-2Rځj$_Xp5rp.:SqX/	5֢;#I93)җLC;Tw?	t⏭EIOy+vQxiDc	t0.QU`CQ	+\V?<M`AJݰrW縗#v|	hj-1nm.&x)*^</Őc(mQ4`ЊCTprŬ_RWG_+nc^C!#x`ܠیt=~;Н>|9btPiNˉm[u}kR_^u2/{?W{qRe3:Q. `똒ौI%ĽY	]<pM_P ,7RqÙF
0Sp'BC^4O`N8[9!-*I)=+2;|!V5nMɂ"{7ABm{*E/!QvzA@"j';F=hI|㛘=<bs/E㎦Iogp_ tz@* YR=09w7ԂI(C$(8 TBZ e9m DT;-hA1!1,?Q;jT*i%Bh?yy    M ;  g    YZ  &               Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пfy9IIMJW\UN	5FCM%O]TTdsDZDQ6<ٞXYBVPoy;*է|^JBbj26oUWmOU:W]ӵ9%_㨥4\Z-:$i$^
ޟVTiTUaQ4WDjQ橴ueFsSVEKyQUB@5
tW%95U[*N 1`5EZDhERdZTElj1MgZtZ$E*Oe[ST}VjWDEU%Q	4%CLU.MM`]q@iE:ɎdfCZ]!"UnSN]MTQXy	F%מѕSYU9vI[njJVVۢEVNiZVjRTSXVAaN뚫/d.Y)tXk-i[^n~u9Iuz릦*jVjkkzV`*jfYU/־&uY%a.VhntnB䭷zRюnU:k~^%zتod)YeHAzjZ8BVmms[^fgj:n:zzaVIxIR{J&؈6XWV7fcWғx:Gi#ih[:_FC韈WהY*%g]DGd殣jv^EKiZUIW^zUTqU$VTuTZRRJjUHTbMgDUUnfeDYQnU[GGo^gII5|X^nDU[da^D+UTSJ),Aܬ-Nl @:7W` =dp(+dx]je<j悕VZ1jU5Tw$T\#XuW53TEM	:410`tZ=8?%
OT`nQl6QsB΀uÎ<4?
|~/	.yFt^BNhS+y*Jx*ޡJ*F[[8!FoF(`(]hxFhR]`	`*xZDTB8-J}b;ӚEzgqx]Nf.cؽ6?1CLÛ 4;P91Hoߣ<EI0Ío~?k}LWlr{<.AqD@;3C3cn!f UΫ A2fNX:#l3lϮhUADIv"jTF8Q#q )]0o?*aީR &hsM{<kXz
{}-TۧIDZcH}]^uu m{>|=WL}>$u3W^^z{]idx(}?
8en	7=%?&)p5ѳd#6^-RfzWPnBo[UJxLF2[:5uU}L mD׺	x:Dg$JCz^Mg9o} H1.G)e7t:|^2EL:PPЛSt3q: W>	/;- W=5>p;v+) ei|O4Ĵ     F  H     )  )       !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  G<?xml version="1.0"?><Features nextValidID="20"><Feature recentID="19" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="5038-5660" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF_nat+MglA-VSVG"/></Q></Feature><Feature recentID="18" name="nat resistance" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5055-5627" color="#a6acb3" type="standard"/><Q name="note"><V text="nat resistance"/></Q></Feature><Feature recentID="17" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8090" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature><Feature recentID="16" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4717-4766" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="15" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="811-843" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="14" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="972-994" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature><Feature recentID="13" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL2"/></Q></Feature><Feature recentID="12" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6290-7147" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="11" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4855-6269" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="10" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2660-3676" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="9" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2235-2654" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="8" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-2012" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="7" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="6" name="cold shock protein" directionality="1" translationMW="131.18" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="67" hitsStopCodon="1"><Segment range="601-607" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="601-607" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="30S ribosomal protein S21" directionality="1" translationMW="7847.44" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="846" maxFusedRunOn="846"><Segment range="607-801" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q></Feature><Feature recentID="3" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="607-801" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="acetyltransferase" directionality="2" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="173" maxRunOn="783" maxFusedRunOn="783"><Segment range="801-801" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="801-801" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-801" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL38 OE_rpsU1R" sequence="atatGCGGCCGCaataaatgctcttctctgagaaattctt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="773-812" boundStrand="1" annealedBases="atatGCGGCCGCaataaatgctcttctctgagaaattctt" meltingTemperature="67"><Component hybridizedRange="773-812" bases="atatGCGGCCGCaataaatgctcttctctgagaaattctt"/></BindingSite><BindingSite simplified="1" location="773-812" boundStrand="1" annealedBases="atatGCGGCCGCaataaatgctcttctctgagaaattctt" meltingTemperature="67"><Component hybridizedRange="773-812" bases="atatGCGGCCGCaataaatgctcttctctgagaaattctt"/></BindingSite></Primer><Primer recentID="1" name="KROL37 OE_rpsU1F" sequence="atatGAATTCaggaggactataatgctaagtattagagtagatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="584-627" boundStrand="0" annealedBases="atatGAATTCaggaggactataatgctaagtattagagtagatg" meltingTemperature="61"><Component hybridizedRange="584-627" bases="atatGAATTCaggaggactataatgctaagtattagagtagatg"/></BindingSite><BindingSite simplified="1" location="584-627" boundStrand="0" annealedBases="atatGAATTCaggaggactataatgctaagtattagagtagatg" meltingTemperature="61"><Component hybridizedRange="584-627" bases="atatGAATTCaggaggactataatgctaagtattagagtagatg"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>fb7696a3-e0ee-4c78-abd1-912d39f9a234</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="22:14:46">2019.2.12</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  #     u  +        +Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пfy9IIMJW\UN	5FCM%O]TTdsDZDQ6<ٞXYBVPoy;*է|^JBbj26oUWmOU:W]ӵ9%_㨥4\Z-:$i$^
ޟVTiTUaQ4WDjQ橴ueFsSVEKyQUB@5
tW%95U[*N 1`5EZDhERdZTElj1MgZtZ$E*Oe[ST}VjWDEU%Q	4%CLU.MM`]q@iE:ɎdfCZ]!"UnSN]MTQXy	F%מѕSYU9vI[njJVVۢEVNiZVjRTSXVAaN뚫/d.Y)tXk-i[^n~u9Iuz릦*jVjkkzV`*jfYU/־&uY%a.VhntnB䭷zRюnU:k~^%zتod)YeHAzjZ8BVmms[^fgj:n:zzaVIxIR{J&؈6XWV7fcWғx:Gi#ih[:_FC韈WהY*%g]DGd殣jv^EKiZUIW^zUTqU$VTuTZRRJjUHTbMgDUUnfeDYQnU[GGo^gII5|X^nDU[da^D+UTSJ),Aܬ-Nl @:7W` =dp(+dx]je<j悕VZ1jU5Tw$T\#XuW53TEM	:410`tZ=8?%
OT`nQl6QsB΀uÎ<4?
|~/08=+8(0hR<ARom50g:ßS6ܞOaQ6x;Pp`>:<Y`klxÀ0|vLξp<ƳL>ĮZPQ&k]ȳk0ڣ;Es%Qm(H
vnL%DEʯXa7T93	5^6wKtU6aQ8_a!xקqAA9|v b^ϡ_aOg!q5]r旃ZY0eJ4z;Ã[<!2mMF	OɲJnw-ydtlxiMGԩ홞hjUjTꡖor+-EQAF̖㎍]U_|**<Q9B^3F}Y<޵|;[H8e,{QJ|C4p ?N1qgLS=οT0t&@]L@N ςnK=2ƪ!@UMs+݊ok||;<1-!10     F  H     )  )  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  N<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7467" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature><Feature recentID="16" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4717-4766" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="15" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="811-843" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="14" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="972-994" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature><Feature recentID="13" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL2"/></Q></Feature><Feature recentID="12" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5667-6524" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="11" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4855-5646" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="10" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2660-3676" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="9" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2235-2654" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="8" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-2012" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="7" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="6" name="cold shock protein" directionality="1" translationMW="131.18" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="67" hitsStopCodon="1"><Segment range="601-607" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="601-607" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="30S ribosomal protein S21" directionality="1" translationMW="7847.44" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="846" maxFusedRunOn="846"><Segment range="607-801" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q></Feature><Feature recentID="3" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="607-801" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="acetyltransferase" directionality="2" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="173" maxRunOn="783" maxFusedRunOn="783"><Segment range="801-801" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="801-801" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-801" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL38 OE_rpsU1R" sequence="atatGCGGCCGCaataaatgctcttctctgagaaattctt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="773-812" boundStrand="1" annealedBases="atatGCGGCCGCaataaatgctcttctctgagaaattctt" meltingTemperature="67"><Component hybridizedRange="773-812" bases="atatGCGGCCGCaataaatgctcttctctgagaaattctt"/></BindingSite><BindingSite simplified="1" location="773-812" boundStrand="1" annealedBases="atatGCGGCCGCaataaatgctcttctctgagaaattctt" meltingTemperature="67"><Component hybridizedRange="773-812" bases="atatGCGGCCGCaataaatgctcttctctgagaaattctt"/></BindingSite></Primer><Primer recentID="1" name="KROL37 OE_rpsU1F" sequence="atatGAATTCaggaggactataatgctaagtattagagtagatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="584-627" boundStrand="0" annealedBases="atatGAATTCaggaggactataatgctaagtattagagtagatg" meltingTemperature="61"><Component hybridizedRange="584-627" bases="atatGAATTCaggaggactataatgctaagtattagagtagatg"/></BindingSite><BindingSite simplified="1" location="584-627" boundStrand="0" annealedBases="atatGAATTCaggaggactataatgctaagtattagagtagatg" meltingTemperature="61"><Component hybridizedRange="584-627" bases="atatGAATTCaggaggactataatgctaagtattagagtagatg"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>fb7696a3-e0ee-4c78-abd1-912d39f9a234</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="22:13:58">2019.2.12</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       t  %        %Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&ПfcD_jEzTSQ:Dd4rTUEFMW=N)TMEEjh͙%eZ|a4&&#kUvSUzu;]U:SEUaٳFO9]eFLeVSNUtFjH\T`55dT(וT$
POzYMޑh?Pi9\eUB
CVT^lUF]&LemeOVƩC^OuLŨDQRJ)mQ6U:U;u@GEo֨XUtDZ)RUBT;hDRڷ-Q4XFFd52/X iv4߄MuaNEHQ)`b]-yU59EQSd@jmjm(y[[l_>d몸Veke%KU:-.jK앒@>ꛪyriպMIEz^YS=$_)[^jjbVffwoV	:a낮j5^nkRmWbVbfgF.Jz]׮.	o&S:V닸Wn}!-BUZ[	dUYz%mV[fg5i6ϖIIgVd'$mia{5iSx.:f5})9ʊkOgv6{c:Qd:n{}yERPz5IvNj=6jd^Dծe^u筕UI*RJkUHgUI*U!U$VTO$ztAUVfTEf jUt{TzVzcWZk^`dIuUK%BUE4bD=V
#~Uz@
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ML_Ӏ3P5I^=A` mFaʳau74+<ka7]l8ȯ-3@3-Gaxq3lp2ӈrxS@x8#4-~&8cX6s9}1]lLM,mm OC͏A,cś`V:ǈ<8md!8Q:1`8|쎧	k4ͳ>J녡Ua&ڼ?>*W8!RUFҀĀl^tQJ\zJ㘃0oɡQ5,gya(IESn6%i#ew=zzΪ`%(N}\q2X5(^ix1yNu`CTA388*hᕸ .&4 `$qט\VGF	x}JٙF^ZAQn}V!*2$UTon8U}0^%M4g՞+H)}Q{7ϝ)= RȺ|
d3L, 
xF~tNa3@ECBo	L4D\&d,
߸z,j 0O\0¾8>خɧϨHN<\|LS      F  H    #  #  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  _<?xml version="1.0"?><Features recycledIDs="9" nextValidID="19"><Feature recentID="8" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="595-795" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="7" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="795-795" color="#00e600" type="standard"/></Feature><Feature recentID="6" name="acetyltransferase" directionality="2" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="173" maxRunOn="777" maxFusedRunOn="777"><Segment range="795-795" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="601-795" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="30S ribosomal protein S21" directionality="1" translationMW="7847.44" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="840" maxFusedRunOn="840"><Segment range="601-795" color="#ff0000" type="standard" translated="1"/><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="595-601" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="cold shock protein" directionality="1" translationMW="131.18" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="67" hitsStopCodon="1"><Segment range="595-601" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1131-2006" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2229-2648" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2654-3670" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4849-5640" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5661-6518" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="966-988" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="805-837" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4711-4760" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7461" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:51:57">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       A  \        \Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пf6ghVFNʵil+קҰИUSNUttNFW)M5VKfαI>旂wՕU1XTeM9UTym"sYQՑR渣C^T~P*MB<U	e6czD}DAMqVʓ)EXQyeVt1?Q[jxd5?Y֝3:EJ*5DVTqZaUhUITBM	PKSSXj{8DQbv2c֗@HU`۔ӗ~U5y9i!D-DQt4eTdEENjFǺfVۚթ袡nQnkU5|Zj.}ک.T贻V'ժ~jgSKY.~KVJn n%byoV6=%e{g]eNd]~*niz驪Z޾սX$醻
ZU{I_mXae+mw^ch&%jNZ.㟧^j%q*
uVohin8%^Vf%ꐕ[imlvuי:+>ZE%&[XU^.:T蒉"եM@Y*+=hHZ:)$Fѐg뺥5iJCQ&9ڝyngVzUמUU$\gUI*U!U$VTuTZUR:>YU[YQfTEUVQz[WRzjRvi_)izV%V/h
UҊa)k+<:S[ ;+ƤUi+rHl*Y {^{_.k@jUuVZk@@0]=/4W-99@ULSBN :vdx*51|N 	BS%x:<4GA*͆`hЮ D99tuc"8 º?A49q3N#-L n;T`c8Άvͱ17'`S豴nM=<^+=36?11mX< "9(9hD0ŀ:&;6Ϯlq+TIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLAL     F  H  Q  Z  Z  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  A<?xml version="1.0"?><Features recycledIDs="2,3,4,5,6,7,8,9" nextValidID="19"><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="930-1805" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2028-2447" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2453-3469" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4648-5439" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5460-6317" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="765-787" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="604-636" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4510-4559" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7260" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:51:53">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                   Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&ПfP)iYn!CNUD
[!3Wd[a,Fh&=eWREfYTYg9QiPiyOjD䓑iS,n	TXQZFF*;pkUQ)3NfuX$TwNVf]E'f6ZTUjTRZP͙%eZ|a4&&#kUvSUzu;]U:SEUaٳFO9]eFLeVSNUtFjH\T`55dT(וT$
POzYMޑh?Pi9\eUB
CVT^lUF]&LemeOVƩC^OuLŨDQRJ)mQ6U:U;u@GEo֨XUtDZ)RUBT;hDRڷ-Q4XFFd52/X iv4߄MuaNEHQ)`b]-yU59EQSd@jmjm(y[[l_>d몸Veke%KU:-.jK앒@>ꛪyriպMIEz^YS=$_)[^jjbVffwoV	:a낮j5^nkRmWbVbfgF.Jz]׮.	o&S:V닸Wn}!-BUZ[	dUYz%mV[fg5i6ϖIIgVd'$mia{5iSx.:f5})9ʊkOgv6{c:Qd:n{}yERPz5IvNj=6jd^Dծe^u筕UI*RJkUHgUI*U!U$VTO$ztAUVfTEf jUt{TzVzcWZk^`dIuUK%BUE4bD=V
#~Uz@
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ML_Ӏ3P5I^=A` mFaʳau74+<ka7]l8ȯ-3@3-Gaxq3lp2ӈrxS@x8#4-~&8cX6s9}1]lLM,mm OC͏A,cś`V:ǈ<8md!8Q:1`8|쎧	k4ͳ>J녡Ua&ڼ?>*W8!RUFҀĀl^tQJ\zJ㘃0oɡQ5,gya(IESn6%i#ew=zzΪ`%(N}\q2X5(^ix1yNu`CTA388*hᕸ .&4 `$qט\VGF	x}JٙF^ZAQn}V!*2$UTon8U}0^%M4g՞+H)}Q{7ϝ)= RȺ|
d3L, 
xF~tNa3@ECBo	L4D\&d,
߸z,j 0O\0¾8>خɧϨHN<\|LS      F  H        !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="19"><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1635-2510" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2733-3152" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3158-4174" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5353-6144" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6165-7022" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1470-1492" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1309-1341" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="9" name="P018 FTL_0552fragF" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="773-799" color="#a020f0" type="standard"/><Q name="note"><V text="P018 FTL_0552fragF"/></Q></Feature><Feature recentID="8" name="P044 INTpEX_FTL0552" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="686-713" color="#a020f0" type="standard"/><Q name="note"><V text="P044 INTpEX_FTL0552"/></Q></Feature><Feature recentID="7" name="P238 FTL_0552F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="616-642" color="#a020f0" type="standard"/><Q name="note"><V text="P238 FTL_0552F"/></Q></Feature><Feature recentID="6" name="P301 PmrA_comp_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="600-627" color="#a020f0" type="standard"/><Q name="note"><V text="P301 PmrA_comp_F"/></Q></Feature><Feature recentID="5" name="P017 FTL_0552fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1272-1299" color="#a020f0" type="standard"/><Q name="note"><V text="P017 FTL_0552fragR"/></Q></Feature><Feature recentID="4" name="FTL_0552" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="616-1299" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0552"/></Q><Q name="db_xref"><V text="3952730" predef="GeneID"/></Q></Feature><Feature recentID="3" name="FTL_0552" directionality="1" translationMW="25520.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1344" maxFusedRunOn="1344"><Segment range="616-1299" color="#993366" type="standard" translated="1"/><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="product"><V text="two-component response regulator"/></Q><Q name="note"><V text="/dnas_title=two-component response regulator"/></Q><Q name="locus_tag"><V text="FTL_0552"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="YP_513318.1"/></Q><Q name="db_xref"><V text="89255956" predef="GI"/><V text="3952730" predef="GeneID"/></Q></Feature><Feature recentID="2" name="P229 PmrADel5R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="600-624" color="#a020f0" type="standard"/><Q name="note"><V text="P229 PmrADel5R"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5215-5264" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7965" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:48:21">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                 fيD* U_JI*h:$kԢ >I~ѽX>]wG
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        7zXZ  ִF !   t/X] 3D].?I*ә_.ZvќP,Vj,ު~mC<ґ8hlFOlY.k(DW`!
 	KJ9rEPܤ$FcprMUq3+Xfg=Gj쭟:o0??o.|ҏ<X7zvTV/0~	{Љ
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"E]bJlf$aPȪ\Vfi,'+!oQUhhZ>$WR]AR`T(
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X/K#z*T:F*\#y4I>@)Qm\C|R3УD6TnCZbLzZQ4M4˳#<BEɳI!meZ`aGeHp3S8N)غxaYMv+'Zrhy5/RTdđU	kF:%4DAсW''5pzxV6gĄW3^첌v[^eցm2tJ*s;&:+;&bDX2XAt;թ$94w組i{l:CP/w6ˑbQiIYD1*7e8bG{k$])_i1&>ET"1iK]Jd.acim
~̵!PWH$$;SƬ4zn~z}*Akww%Y#a&P]F*oҿt6ӲqX>űy3  kf" 	   g
1g    YZ                   Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пfy9IIMJW\UN	5FCM%O]TTdsDZDQ6<ٞXYBVPoy;*է|^JBbj26oUWmOU:W]ӵ9%_㨥4\Z-:$i$^
ޟVTiTUaQ4WDjQ橴ueFsSVEKyQUB@5
tW%95U[*N 1`5EZDhERdZTElj1MgZtZ$E*Oe[ST}VjWDEU%Q	4%CLU.MM`]q@iE:ɎdfCZ]!"UnSN]MTQXy	F%מѕSYU9vI[njJVVۢEVNiZVjRTSXVAaN뚫/d.Y)tXk-i[^n~u9Iuz릦*jVjkkzV`*jfYU/־&uY%a.VhntnB䭷zRюnU:k~^%zتod)YeHAzjZ8BVmms[^fgj:n:zzaVIxIR{J&؈6XWV7fcWғx:Gi#ih[:_FC韈WהY*%g]DGd殣jv^EKiZUIW^zUTqU$VTuTZRRJjUHTbMgDUUnfeDYQnU[GGo^gII5|X^nDU[da^D+UTSJ),Aܬ-Nl @:7W` =dp(+dx]je<j悕VZ1jU5Tw$T\#XuW53TEM	:410`tZ=8?%
OT`nQl6QsB΀uÎ<4?
|~/	.yFt^BNhS+y*Jx*ޡJ*F[[8!FoF(`(]hxFhR]`	`*xZDTB8-J}b;ӚEzgqx]Nf.cؽ6?11mX< "9(9hD0ŀ:&;6Ϯlq+TIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLAL     F  H     )  )       7zXZ  ִF !   t/] 3D].?I*ә_.ZvќP,Vj,ު~mC<ґ?vd;!U''yzYl.vU[Qec <L3cGq~=X?r+<ɵؿĊG]1|=ty?izd@Ppp}ݽ<,c^D2)=0m(w+}[)0|cK7tDi|6?i6J$MEԎ.j7ȡdw}fHAKnm/<`!H|r,7\LD'j ?.|'J&29?J EՋ%1DiFu?\mo]\`[SsGrnڦ"$X=i`hzЉwA<J$"%N1]I11Ҁz_yjs(J:tI
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ibl">,Ѣ?=	GԵZ`k`jǨ9.p*G	X+{	i%Y%NX%/W?S/;Wy;)OEqe 553O$lPqw#<G7.Ml3+&!%2Gs?Ř)
M2	q:Ps/sa*,2ko8<\,_KjA^ZmYq&_R|W08|Ft`[3r	sd5sNr0 ֆp"׎p DL܀d_-2Rځj$_Xp5rp.:SqX/	5֢;#I93)җLC;Tw?	t⏭EIOy+vQxiDc	t0.QU`CQ	+\V?<M`AJݰrW縗#v|	hj-1nm.&x)*^</Őc(mQ4`ЊCTprŬ_RWG_+nc^C!#x`ܠیt=~;Н>|9btPiNˉm[u}kR_^u2/{?W{qRe3:Q. `똒ौI%ĽY	]<pM_P ,7RqÙF
0Sp'BC^4O`N8[9!-*I)=+2;|!V5nMɂ"{7ABm{*E/!QvzA@"j';F=hI|㛘=<bs/E㎦Iogp_ tz@* YR=09w7ԂI(C$(8 TBZ e9m DT;-hA1!1,?Q;jT*i%Bh?yy    M ;  g    YZ  &               Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пfy9IIMJW\UN	5FCM%O]TTdsDZDQ6<ٞXYBVPoy;*է|^JBbj26oUWmOU:W]ӵ9%_㨥4\Z-:$i$^
ޟVTiTUaQ4WDjQ橴ueFsSVEKyQUB@5
tW%95U[*N 1`5EZDhERdZTElj1MgZtZ$E*Oe[ST}VjWDEU%Q	4%CLU.MM`]q@iE:ɎdfCZ]!"UnSN]MTQXy	F%מѕSYU9vI[njJVVۢEVNiZVjRTSXVAaN뚫/d.Y)tXk-i[^n~u9Iuz릦*jVjkkzV`*jfYU/־&uY%a.VhntnB䭷zRюnU:k~^%zتod)YeHAzjZ8BVmms[^fgj:n:zzaVIxIR{J&؈6XWV7fcWғx:Gi#ih[:_FC韈WהY*%g]DGd殣jv^EKiZUIW^zUTqU$VTuTZRRJjUHTbMgDUUnfeDYQnU[GGo^gII5|X^nDU[da^D+UTSJ),Aܬ-Nl @:7W` =dp(+dx]je<j悕VZ1jU5Tw$T\#XuW53TEM	:410`tZ=8?%
OT`nQl6QsB΀uÎ<4?
|~/	.yFt^BNhS+y*Jx*ޡJ*F[[8!FoF(`(]hxFhR]`	`*xZDTB8-J}b;ӚEzgqx]Nf.cؽ6?1CLÛ 4;P91Hoߣ<EI0Ío~?k}LWlr{<.AqD@;3C3cn!f UΫ A2fNX:#l3lϮhUADIv"jTF8Q#q )]0o?*aީR &hsM{<kXz
{}-TۧIDZcH}]^uu m{>|=WL}>$u3W^^z{]idx(}?
8en	7=%?&)p5ѳd#6^-RfzWPnBo[UJxLF2[:5uU}L mD׺	x:Dg$JCz^Mg9o} H1.G)e7t:|^2EL:PPЛSt3q: W>	/;- W=5>p;v+) ei|O4Ĵ     F  H     )  )       !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  G<?xml version="1.0"?><Features nextValidID="20"><Feature recentID="19" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="5038-5660" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF_nat+MglA-VSVG"/></Q></Feature><Feature recentID="18" name="nat resistance" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5055-5627" color="#a6acb3" type="standard"/><Q name="note"><V text="nat resistance"/></Q></Feature><Feature recentID="17" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8090" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature><Feature recentID="16" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4717-4766" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="15" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="811-843" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="14" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="972-994" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature><Feature recentID="13" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL2"/></Q></Feature><Feature recentID="12" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6290-7147" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="11" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4855-6269" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="10" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2660-3676" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="9" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2235-2654" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="8" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-2012" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="7" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="6" name="cold shock protein" directionality="1" translationMW="131.18" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="67" hitsStopCodon="1"><Segment range="601-607" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="601-607" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="30S ribosomal protein S21" directionality="1" translationMW="7847.44" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="846" maxFusedRunOn="846"><Segment range="607-801" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q></Feature><Feature recentID="3" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="607-801" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="acetyltransferase" directionality="2" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="173" maxRunOn="783" maxFusedRunOn="783"><Segment range="801-801" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="801-801" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-801" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL38 OE_rpsU1R" sequence="atatGCGGCCGCaataaatgctcttctctgagaaattctt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="773-812" boundStrand="1" annealedBases="atatGCGGCCGCaataaatgctcttctctgagaaattctt" meltingTemperature="67"><Component hybridizedRange="773-812" bases="atatGCGGCCGCaataaatgctcttctctgagaaattctt"/></BindingSite><BindingSite simplified="1" location="773-812" boundStrand="1" annealedBases="atatGCGGCCGCaataaatgctcttctctgagaaattctt" meltingTemperature="67"><Component hybridizedRange="773-812" bases="atatGCGGCCGCaataaatgctcttctctgagaaattctt"/></BindingSite></Primer><Primer recentID="1" name="KROL37 OE_rpsU1F" sequence="atatGAATTCaggaggactataatgctaagtattagagtagatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="584-627" boundStrand="0" annealedBases="atatGAATTCaggaggactataatgctaagtattagagtagatg" meltingTemperature="61"><Component hybridizedRange="584-627" bases="atatGAATTCaggaggactataatgctaagtattagagtagatg"/></BindingSite><BindingSite simplified="1" location="584-627" boundStrand="0" annealedBases="atatGAATTCaggaggactataatgctaagtattagagtagatg" meltingTemperature="61"><Component hybridizedRange="584-627" bases="atatGAATTCaggaggactataatgctaagtattagagtagatg"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>fb7696a3-e0ee-4c78-abd1-912d39f9a234</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="22:14:46">2019.2.12</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  #     u  +        +Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пfy9IIMJW\UN	5FCM%O]TTdsDZDQ6<ٞXYBVPoy;*է|^JBbj26oUWmOU:W]ӵ9%_㨥4\Z-:$i$^
ޟVTiTUaQ4WDjQ橴ueFsSVEKyQUB@5
tW%95U[*N 1`5EZDhERdZTElj1MgZtZ$E*Oe[ST}VjWDEU%Q	4%CLU.MM`]q@iE:ɎdfCZ]!"UnSN]MTQXy	F%מѕSYU9vI[njJVVۢEVNiZVjRTSXVAaN뚫/d.Y)tXk-i[^n~u9Iuz릦*jVjkkzV`*jfYU/־&uY%a.VhntnB䭷zRюnU:k~^%zتod)YeHAzjZ8BVmms[^fgj:n:zzaVIxIR{J&؈6XWV7fcWғx:Gi#ih[:_FC韈WהY*%g]DGd殣jv^EKiZUIW^zUTqU$VTuTZRRJjUHTbMgDUUnfeDYQnU[GGo^gII5|X^nDU[da^D+UTSJ),Aܬ-Nl @:7W` =dp(+dx]je<j悕VZ1jU5Tw$T\#XuW53TEM	:410`tZ=8?%
OT`nQl6QsB΀uÎ<4?
|~/08=+8(0hR<ARom50g:ßS6ܞOaQ6x;Pp`>:<Y`klxÀ0|vLξp<ƳL>ĮZPQ&k]ȳk0ڣ;Es%Qm(H
vnL%DEʯXa7T93	5^6wKtU6aQ8_a!xקqAA9|v b^ϡ_aOg!q5]r旃ZY0eJ4z;Ã[<!2mMF	OɲJnw-ydtlxiMGԩ홞hjUjTꡖor+-EQAF̖㎍]U_|**<Q9B^3F}Y<޵|;[H8e,{QJ|C4p ?N1qgLS=οT0t&@]L@N ςnK=2ƪ!@UMs+݊ok||;<1-!10     F  H     )  )  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  N<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7467" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature><Feature recentID="16" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4717-4766" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="15" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="811-843" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="14" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="972-994" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature><Feature recentID="13" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL2"/></Q></Feature><Feature recentID="12" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5667-6524" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="11" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4855-5646" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="10" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2660-3676" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="9" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2235-2654" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="8" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-2012" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="7" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="6" name="cold shock protein" directionality="1" translationMW="131.18" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="67" hitsStopCodon="1"><Segment range="601-607" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="601-607" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="30S ribosomal protein S21" directionality="1" translationMW="7847.44" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="846" maxFusedRunOn="846"><Segment range="607-801" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q></Feature><Feature recentID="3" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="607-801" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="acetyltransferase" directionality="2" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="173" maxRunOn="783" maxFusedRunOn="783"><Segment range="801-801" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="801-801" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-801" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL38 OE_rpsU1R" sequence="atatGCGGCCGCaataaatgctcttctctgagaaattctt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="773-812" boundStrand="1" annealedBases="atatGCGGCCGCaataaatgctcttctctgagaaattctt" meltingTemperature="67"><Component hybridizedRange="773-812" bases="atatGCGGCCGCaataaatgctcttctctgagaaattctt"/></BindingSite><BindingSite simplified="1" location="773-812" boundStrand="1" annealedBases="atatGCGGCCGCaataaatgctcttctctgagaaattctt" meltingTemperature="67"><Component hybridizedRange="773-812" bases="atatGCGGCCGCaataaatgctcttctctgagaaattctt"/></BindingSite></Primer><Primer recentID="1" name="KROL37 OE_rpsU1F" sequence="atatGAATTCaggaggactataatgctaagtattagagtagatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="584-627" boundStrand="0" annealedBases="atatGAATTCaggaggactataatgctaagtattagagtagatg" meltingTemperature="61"><Component hybridizedRange="584-627" bases="atatGAATTCaggaggactataatgctaagtattagagtagatg"/></BindingSite><BindingSite simplified="1" location="584-627" boundStrand="0" annealedBases="atatGAATTCaggaggactataatgctaagtattagagtagatg" meltingTemperature="61"><Component hybridizedRange="584-627" bases="atatGAATTCaggaggactataatgctaagtattagagtagatg"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>fb7696a3-e0ee-4c78-abd1-912d39f9a234</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="22:13:58">2019.2.12</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       t  %        %Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&ПfcD_jEzTSQ:Dd4rTUEFMW=N)TMEEjh͙%eZ|a4&&#kUvSUzu;]U:SEUaٳFO9]eFLeVSNUtFjH\T`55dT(וT$
POzYMޑh?Pi9\eUB
CVT^lUF]&LemeOVƩC^OuLŨDQRJ)mQ6U:U;u@GEo֨XUtDZ)RUBT;hDRڷ-Q4XFFd52/X iv4߄MuaNEHQ)`b]-yU59EQSd@jmjm(y[[l_>d몸Veke%KU:-.jK앒@>ꛪyriպMIEz^YS=$_)[^jjbVffwoV	:a낮j5^nkRmWbVbfgF.Jz]׮.	o&S:V닸Wn}!-BUZ[	dUYz%mV[fg5i6ϖIIgVd'$mia{5iSx.:f5})9ʊkOgv6{c:Qd:n{}yERPz5IvNj=6jd^Dծe^u筕UI*RJkUHgUI*U!U$VTO$ztAUVfTEf jUt{TzVzcWZk^`dIuUK%BUE4bD=V
#~Uz@
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ML_Ӏ3P5I^=A` mFaʳau74+<ka7]l8ȯ-3@3-Gaxq3lp2ӈrxS@x8#4-~&8cX6s9}1]lLM,mm OC͏A,cś`V:ǈ<8md!8Q:1`8|쎧	k4ͳ>J녡Ua&ڼ?>*W8!RUFҀĀl^tQJ\zJ㘃0oɡQ5,gya(IESn6%i#ew=zzΪ`%(N}\q2X5(^ix1yNu`CTA388*hᕸ .&4 `$qט\VGF	x}JٙF^ZAQn}V!*2$UTon8U}0^%M4g՞+H)}Q{7ϝ)= RȺ|
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߸z,j 0O\0¾8>خɧϨHN<\|LS      F  H    #  #  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  _<?xml version="1.0"?><Features recycledIDs="9" nextValidID="19"><Feature recentID="8" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="595-795" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="7" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="795-795" color="#00e600" type="standard"/></Feature><Feature recentID="6" name="acetyltransferase" directionality="2" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="173" maxRunOn="777" maxFusedRunOn="777"><Segment range="795-795" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="601-795" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="30S ribosomal protein S21" directionality="1" translationMW="7847.44" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="840" maxFusedRunOn="840"><Segment range="601-795" color="#ff0000" type="standard" translated="1"/><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="595-601" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="cold shock protein" directionality="1" translationMW="131.18" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="67" hitsStopCodon="1"><Segment range="595-601" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1131-2006" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2229-2648" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2654-3670" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4849-5640" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5661-6518" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="966-988" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="805-837" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4711-4760" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7461" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:51:57">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       A  \        \Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пf6ghVFNʵil+קҰИUSNUttNFW)M5VKfαI>旂wՕU1XTeM9UTym"sYQՑR渣C^T~P*MB<U	e6czD}DAMqVʓ)EXQyeVt1?Q[jxd5?Y֝3:EJ*5DVTqZaUhUITBM	PKSSXj{8DQbv2c֗@HU`۔ӗ~U5y9i!D-DQt4eTdEENjFǺfVۚթ袡nQnkU5|Zj.}ک.T贻V'ժ~jgSKY.~KVJn n%byoV6=%e{g]eNd]~*niz驪Z޾սX$醻
ZU{I_mXae+mw^ch&%jNZ.㟧^j%q*
uVohin8%^Vf%ꐕ[imlvuי:+>ZE%&[XU^.:T蒉"եM@Y*+=hHZ:)$Fѐg뺥5iJCQ&9ڝyngVzUמUU$\gUI*U!U$VTuTZUR:>YU[YQfTEUVQz[WRzjRvi_)izV%V/h
UҊa)k+<:S[ ;+ƤUi+rHl*Y {^{_.k@jUuVZk@@0]=/4W-99@ULSBN :vdx*51|N 	BS%x:<4GA*͆`hЮ D99tuc"8 º?A49q3N#-L n;T`c8Άvͱ17'`S豴nM=<^+=36?11mX< "9(9hD0ŀ:&;6Ϯlq+TIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLAL     F  H  Q  Z  Z  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  A<?xml version="1.0"?><Features recycledIDs="2,3,4,5,6,7,8,9" nextValidID="19"><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="930-1805" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2028-2447" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2453-3469" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4648-5439" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5460-6317" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="765-787" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="604-636" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4510-4559" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7260" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:51:53">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                   Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&ПfP)iYn!CNUD
[!3Wd[a,Fh&=eWREfYTYg9QiPiyOjD䓑iS,n	TXQZFF*;pkUQ)3NfuX$TwNVf]E'f6ZTUjTRZP͙%eZ|a4&&#kUvSUzu;]U:SEUaٳFO9]eFLeVSNUtFjH\T`55dT(וT$
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CVT^lUF]&LemeOVƩC^OuLŨDQRJ)mQ6U:U;u@GEo֨XUtDZ)RUBT;hDRڷ-Q4XFFd52/X iv4߄MuaNEHQ)`b]-yU59EQSd@jmjm(y[[l_>d몸Veke%KU:-.jK앒@>ꛪyriպMIEz^YS=$_)[^jjbVffwoV	:a낮j5^nkRmWbVbfgF.Jz]׮.	o&S:V닸Wn}!-BUZ[	dUYz%mV[fg5i6ϖIIgVd'$mia{5iSx.:f5})9ʊkOgv6{c:Qd:n{}yERPz5IvNj=6jd^Dծe^u筕UI*RJkUHgUI*U!U$VTO$ztAUVfTEf jUt{TzVzcWZk^`dIuUK%BUE4bD=V
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ML_Ӏ3P5I^=A` mFaʳau74+<ka7]l8ȯ-3@3-Gaxq3lp2ӈrxS@x8#4-~&8cX6s9}1]lLM,mm OC͏A,cś`V:ǈ<8md!8Q:1`8|쎧	k4ͳ>J녡Ua&ڼ?>*W8!RUFҀĀl^tQJ\zJ㘃0oɡQ5,gya(IESn6%i#ew=zzΪ`%(N}\q2X5(^ix1yNu`CTA388*hᕸ .&4 `$qט\VGF	x}JٙF^ZAQn}V!*2$UTon8U}0^%M4g՞+H)}Q{7ϝ)= RȺ|
d3L, 
xF~tNa3@ECBo	L4D\&d,
߸z,j 0O\0¾8>خɧϨHN<\|LS      F  H        !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="19"><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1635-2510" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2733-3152" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3158-4174" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5353-6144" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6165-7022" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1470-1492" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1309-1341" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="9" name="P018 FTL_0552fragF" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="773-799" color="#a020f0" type="standard"/><Q name="note"><V text="P018 FTL_0552fragF"/></Q></Feature><Feature recentID="8" name="P044 INTpEX_FTL0552" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="686-713" color="#a020f0" type="standard"/><Q name="note"><V text="P044 INTpEX_FTL0552"/></Q></Feature><Feature recentID="7" name="P238 FTL_0552F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="616-642" color="#a020f0" type="standard"/><Q name="note"><V text="P238 FTL_0552F"/></Q></Feature><Feature recentID="6" name="P301 PmrA_comp_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="600-627" color="#a020f0" type="standard"/><Q name="note"><V text="P301 PmrA_comp_F"/></Q></Feature><Feature recentID="5" name="P017 FTL_0552fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1272-1299" color="#a020f0" type="standard"/><Q name="note"><V text="P017 FTL_0552fragR"/></Q></Feature><Feature recentID="4" name="FTL_0552" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="616-1299" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0552"/></Q><Q name="db_xref"><V text="3952730" predef="GeneID"/></Q></Feature><Feature recentID="3" name="FTL_0552" directionality="1" translationMW="25520.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1344" maxFusedRunOn="1344"><Segment range="616-1299" color="#993366" type="standard" translated="1"/><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="product"><V text="two-component response regulator"/></Q><Q name="note"><V text="/dnas_title=two-component response regulator"/></Q><Q name="locus_tag"><V text="FTL_0552"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="YP_513318.1"/></Q><Q name="db_xref"><V text="89255956" predef="GI"/><V text="3952730" predef="GeneID"/></Q></Feature><Feature recentID="2" name="P229 PmrADel5R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="600-624" color="#a020f0" type="standard"/><Q name="note"><V text="P229 PmrADel5R"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5215-5264" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7965" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:48:21">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                 fيD* U_JI*h:$kԢ >I~ѽX>]wG
չXdUM4'MziNU7@ igX$ҧ;U(`UosCX!Tn-\њ`X䗹C.T0;uBԧ+'%IZF~:h-QJ{MN83TJfM`YvӖjnb];JgR*BXjVg](;* R	YJ}cLeygXt5U1$kՙ;RzBk YtjZWNkX^yIjX,Xڍ'SMڕE\kXk=:ӭFժIQbl/5N\Yޝn*f          
        7zXZ  ִF !   t/X] 3D].?I*ә_.ZvќP,Vj,ު~mC<ґ8hlFOlY.k(DW`!
 	KJ9rEPܤ$FcprMUq3+Xfg=Gj쭟:o0??o.|ҏ<X7zvTV/0~	{Љ
(5nJgȰ&`Ez#O')XivaR|R(UG`׳"H@<D%ȝbqcV46ӥ/|1ԴsBj3)^BNS:o@qF*͍hJ?m&,H΍s4(DI+z	o^BCb,/ޥs9UWqgJl{.0cސQ(U4\MZ
"E]bJlf$aPȪ\Vfi,'+!oQUhhZ>$WR]AR`T(
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1g    YZ  	07zXZ  ִF !   t/N] ˇf{3(1v%f M*	mj)p80*i	NY]QF7&!na!ڠ)CEt:lJc}qQud\]LF-!ep!h]b^ <tz_.AZBw, x,L#38*\X}[Le 2!mϞ QmܐKz4@ES@k. "Q/wtG(bʅa Wq{ޞe!E.BOcyR<# "B_}WKAvUtċsINm:sRjg!}k} <C4[3sx­5ӔX3|NTZqXbt=!$%yytpd)#D o􌮻&#=!^&jܩV-!Ɩ7=GUx)*$X#b9s!ܡJvd'DlQG|h.˞O1n\z|%*PWoEpkSw~ I_S3 G4Z.yCʭUFK-Rvaww:V^[%[
t[AT\wTLj]3@yH9U5G  }m1Pj	7e:5̱{QÀ=];?XSum}@3䰤kFnT _Xmn\FNPhdƏ1{ᰍEpJ*\<2Tv7|nX̰3 jd+,1e@.XCQ|?D3tu4Q};[ݹ<ּmO
VoCR޵)vL7#	=&g<8v=eN:mEZp	0v/^"F#+ہ;R[@UdEi;>fX¨rXٌZKkIഃ|,{( +OIkSG 	DݒPI>y{FkTѾ8%j'VO X@j/^p>ب3t^0qMdBTh:߈LT*bXoʳm(˪¨>OArM7HG5Q?o1eX >J,1Z<D%PuΛ$ďH2C!Xab\bڭ\vVniV}N!2JqbUB#"=UN~ίn7A?Ro#+^ ݴn58Q.'&gBp^Pd9A6k[7`5Mcgǣo51mrfYy2^6hCۗAUơ>4MX"s_
>*#MټЩ SbnXQ|U/M9d)V{fikWM::rJx-`B썯a}Af&U녻4i\"94Z2D.!?pH!;4-6BF
>scJ%	w2"]AZfM-ޓ$σ
=3:ϖnT͕R(>ܙd½#|Jϫ#V)DL;|0J&|d)#^u΂hf:f- IgDjErcd
*߮XMݕ8kEF̝	-}Zb̲V[ue:uX&?HO膈r`,m|$E10tŮN	lsB#M}JG%ScBISuQ2[42^{F~wĐv.Rx)6G{ԂHx D>ҩi2QNݗL%R`kҩfKZfwCpӇDkBČ*(5mU3ee{s˼fxUToÃR!WI@J4
x*zMn}UTb,&bϪH$)"b=yZƩՀO?)t$XumVK>T Sopu^kⷂX5۳f}"qvڑ9/oLG1dOWpV`0Ҥ     'Qv  ̭og    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ^<?xml version="1.0"?><Features nextValidID="12"><Feature recentID="0" name="nat resistance" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6472-7044" color="#a6acb3" type="standard"/><Q name="note"><V text="nat resistance"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6134-6183" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="2" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2389-2411" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature><Feature recentID="3" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL2"/></Q></Feature><Feature recentID="4" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7329-8186" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="5" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4077-5093" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="6" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3652-4071" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="7" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2554-3429" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="8" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="9" name="FTL_0439" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="608-2263" color="#00e600" type="standard"/></Feature><Feature recentID="10" name="FupA/B" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="608-2263" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="VVRKFKKTCLIVSSLLACSGLAYSEDSPQVVSQGGPLGATSIGDQNLGQPDPNASGASSTTQTTGSNLNDRELLLKLQQQVQQLQGQLQQLKAQGNGGGLQNTSNGSSQFTTYSSKVDGNKNPRTLGGNGESKDLSQALIGGQTSSDIMGNVNASNSIINLASEPLGGVFNQKGGIDVGGAPAITTQGQVTYLGSYSGNNSIPIGQISSNLFASTLLGQREKFDDYSVFFGGFIEADAQAWFGSAVTKAQSAGQLYSNGQNIYLTSANLYFLSNLGHYVTAQFDFDTNESGSFSLGNAFVIFGNLDTTPVFVSVGKYRPSVGSFGGGGPWTSGITANMFRPLRVTNAAINYRGDTSNANFTVFDAKNHATFSVAYFDAVSIPNIAQVGFNLGYMHDIRGANNRFNFIDKRVGEFNIDTAISFESIPFLPGNLNVGAGWATTTTQSTQFNGRSNAFAGAFTVQAAYTFKLFGSGQNINASYGHSYNADNIPMPLSAGGSFFLAASGIKDQILVSTQRSFFDDNVLIGPEYSWQSLYNGQRMNTLTLDLSVYI*"/></Q></Feature><Feature recentID="11" name="DR36 Tn insertion site" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="892-893" color="#a6acb3" type="standard"/></Feature></Features>
  	<?xml version="1.0"?><Primers nextValidID="3"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="KROL442  FTL_0439_PCR_F" sequence="atgatagggaacttttgctaaaattacagcagcaggtacagcaacttcagggacaattacaacagctaaaagcacaggg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2021-05-25T13:44:23Z"><BindingSite location="812-890" boundStrand="0" annealedBases="atgatagggaacttttgctaaaattacagcagcaggtacagcaacttcagggacaattacaacagctaaaagcacaggg" meltingTemperature="73"><Component hybridizedRange="812-890" bases="atgatagggaacttttgctaaaattacagcagcaggtacagcaacttcagggacaattacaacagctaaaagcacaggg"/></BindingSite><BindingSite simplified="1" location="812-890" boundStrand="0" annealedBases="atgatagggaacttttgctaaaattacagcagcaggtacagcaacttcagggacaattacaacagctaaaagcacaggg" meltingTemperature="73"><Component hybridizedRange="812-890" bases="atgatagggaacttttgctaaaattacagcagcaggtacagcaacttcagggacaattacaacagctaaaagcacaggg"/></BindingSite></Primer><Primer recentID="1" name="KROL449  OE_fupAB_F" sequence="atatCATATGttagagggaagttttactgtggtgc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2021-05-25T14:20:20Z"><BindingSite location="583-613" boundStrand="0" annealedBases="CATATGttagagggaagttttactgtggtgc" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="583-613" bases="CATATGttagagggaagttttactgtggtgc"/></BindingSite><BindingSite simplified="1" location="583-613" boundStrand="0" annealedBases="CATATGttagagggaagttttactgtggtgc" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="583-613" bases="CATATGttagagggaagttttactgtggtgc"/></BindingSite></Primer><Primer recentID="2" name="KROL450  OE_fupAB_R" sequence="atatGGATCCctagatataaactgaaagatctaatgtaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2021-05-25T14:20:20Z"><BindingSite location="2234-2269" boundStrand="1" annealedBases="tGGATCCctagatataaactgaaagatctaatgtaa" meltingTemperature="58"><Component bases="ata"/><Component hybridizedRange="2234-2269" bases="tGGATCCctagatataaactgaaagatctaatgtaa"/></BindingSite><BindingSite simplified="1" location="2234-2269" boundStrand="1" annealedBases="tGGATCCctagatataaactgaaagatctaatgtaa" meltingTemperature="58"><Component bases="ata"/><Component hybridizedRange="2234-2269" bases="tGGATCCctagatataaactgaaagatctaatgtaa"/></BindingSite></Primer></Primers>
  i<Notes>
<UUID>b18b2a96-9048-4641-adf7-ab84a9fc118f</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="14:21:31">2021.5.25</Created>
<LastModified UTC="14:22:37">2021.5.25</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         6+ Cutters                                                                                                   d     CHO  d              E              Y? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
