	   SnapGene      GCTAGTGGTGCTAGCCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGGGATCCttatttatataattcatccataccatgagtaataccagcagcagtaacaaattctaataaaaccatatgatctcttttttcatttggatcttttgataaaactgattgagttgataaataatgattatctggtaataaaactggaccatcaccaattggagtattttgttgataatgatcagctaattgaactgaaccatcttcaacattatgtctaattttaaaattagctttaataccatttttttgtttatcagcagtaatataaacattatgtgaattaaaattatattctaatttatgacctaaaatattaccatcttctttaaaatcaataccttttaattcaattctattaactaaagtatcaccttcaaatttaacttcagctctagttttataagtaccatcatctttaaatgaaatagttctttcttgaacataaccttctggcatagctgatttaaaaaaatcatgtcttttcatatgatctggatatcttgaaaaacattgaacaccataagttaaagtagtaactaaagttggccatggaactggtaatttaccagtagtacaaataaattttaaagttaatttaccattagtagcatcaccttcaccttcacctctaactgaaaatttatgaccattaacatcaccatctaattcaactaaaattggaacaacaccagtaaataattcttcaccttttgaaaccatatagatactcctatttttaaatattcacatatactcCCTATAGTGAGTCGTATTACAGCTGATaatATGTCGACGGTACCGCTCTTCTTAATACGACTCACTATAGGgagtatatgtgaatatttaaaaataggagtatctatatgaaaaaaataattaagcttagtcttttatctttatcaatcgcaggtttagcgagctgttctactctagggttaggtggctctgatgatgcaaaagcttcagctaaagatactgctgctgctcagacagctactactgagcaagctgctgctgtatctaagccaactgcaaaagtaagtttaaataaacttggtcaggataaaataaaagcaactgtatatacagcatacaataataacccacaaggaagtgtaagattacaatggcaggctccagaaggttctaagtgccatgatacaagcttcccaattactaagtatgctgagaagaacgataaaacttgggcaactgtaacagttaagcaaggtaataacttctgtagcggtaagtggacagctaatgtagtttatgacaaagaagtaatcgcttctgattcaataaatatttaatttcttcaaatatttataattttctagtttttgcttttctcaaaaaaaaaCCCGGGCCATGGCTCGAGGCTAGCGGTCCCGGGGGATCGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGAAGCTTGGCACTGGCCGACCGGGGTCGAGCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGCTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACAGATCCGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGT  !   
   
   	                  
               
                                       
               	            	                                 
      
   
      
         	                                                                                             C            %      	                              "         
                                                      	                     
                  	            	      	      	   
               &                                             	               !   '      
                                                      	   
               
         
                                                                     
                           	      
                        	                  	                  	              
 2 "Xj	
 > ~	`

j7	 3 #Yk	 d

?  3a
 k6Bm{	
  8H ?  2isJ			k  +O
  >	E!  q_$-l	X 4 $ 	m dZsl			
  A`}I_@
 J_	
=$ {e

] * ePq

 (  4<
b
 	
: _   A<	$P .^|
 ECnS		

!r ;/DB		I Z<9Q El7	|	     3QjYR
[

l
   Tt7			
 @    - 	K	V	^				"
w \/q	 !,?KL	C sePQB
	   sT
L  )?	y	
C X?
" M
FA	  yx
 V}6	O
 a p  HYrR%H.m	
  F=|	YC `&   b;

+.RN 5 P		 # =V9%c!	)		 T w0
n CY[tJ
Q e8	H 2b
 !7m		'

 >

 "	G
 B~ 

~JA a`	
 m{"=	
$u j m E`cy1	4	?	
 0Lv	-	}


&%/	 	 !>u K`
 N47:,j|	z
   	 :  
%0G7 *NF
f
^ eo2q}t	

MhU Q


,  fEYf
1w $>

 + r
[ 1 = }!c

6>~ h25NrI		 = ) _
<|# ' ;{ DA	R	
; X6	U				Q

   K
  >(x	
w

@ ` o  GUX|QG			%  OaU8		:
Q B7=
1 !}	

 i l D/u_ bx0	3	
~t  )M	$3/Epg  De

 0 1H		
{"5=  CWJQ	T	 NT`PT'F6		
0 2CtDo/	s S_OsB0VG			4C       #_x<= Nmr()C	D	E	F						'123 u  $`y'Zn\m*			(J     O>^E				
   3	

)K% c |   /Jz(Tn[oY]n
=eo:Ri O v   %[altdw	
	 J+		

 }	(t r P?Fo		   [mH_x80		U

    F
B	
, i[ ]    q+=			
 VW4L 8 
E* <{8
>|" M }      MLR\kpZuTS

m; K  Ke{)4)1U~^o<		j X d 03oZ\

fpSY
    B
4o  &S\b}Hu8			
l G   5mu!`eqx	+

w P w g+Uc5 K,4V@   A#		

x{    .6?L	_			
#
m
 A T >)	W			4


 ]h	^
hF qO~	Y
u ) 0;	r	
K K"-Lp		: s @&
	 Q@/z GMD   n+Iy Mb\i \x(9Q1V
	 `R	
 5tf	C
(U[ bU	

Mr Z f    2Iy\	 
j

- t	
   GC
 
    1j*lz	   pck				
DW  dIO@^p
\ ^  @-;		
#9  Y:D.	Pq        ,PS]	
	 r>dp		A
 W  <{5N
B  <(	
-M	  X&
G l~z!#F	 K,	|

  9 69+
y
6T	 =

0Z+
 < | & 	P
  W~=7~9
?  ( w	} MBS&I<& ~    "N^wR^&Y]lq[U					
 N b {     .IksSm2Icv	

n q Z/nT
 7  Up*V<	ZD J L Ld2;z}t;S		 F  f| *G_p*

kn  @5>=(3U	


z p:	g	X
]
g J9 4'*'TZ Y e 1xa[	i

q   cb
H		V      9C:\	Op  ;'	
, *+	<

"/5SY	 % 'v !-]Q%lX
b			

 6 E I  ?Tco;s'T	

my ~wI9		h
    :
)&	*	[		!U! $ H  ,>nv$W:ar		

x'      6789$	"#y&'("	#	$	%	b				cd  %fzdc	,
e
 x-
 Q  &,Vj	1
`df x h iPg{d	6 eo_ qy Z\- Duw5.
K
AOR LhW  Bf9v	


e|  Wk4O			y*cg R  '	2
a3W  -v$		
I  /Qn		$
n
7 y i@|e		(

   "7gjh8`		W]  M	
 ?*		
j	 U ?5



	 B X		
`
 7fsE
p Wi		_

i A
G ^#r,	l z	
H^
 ]

O C	 !
Z
v
 [rS.JPKB xL
B E " vb/
*S
 6I
Pa <	
t >	
%. dMin1|F	s	
L #X
v gM^q;} :z5	@		

 n F{w		
v

 h k  CL#.ad2	



f  / 15.	P	z/  t MAl'			&0  X	
~

dh GwA
's+ \ h	 W  
(K0
7
{X  4RA3b4v S  
"3
l

? N
JE .%	y
 wLa[h  H		

   0oNk]j	o
  O,R-co		@
8 ;z%
 58Jk} {

  
 ; }	 z  jv]A1H	 :y:R2
)

	 &)f	W
8 8a	G9	X
 D k&S
g
   +#Z	a		
 b hOi	_^ gpvN  u3N	u		

)V  f~V\ 6rD


i @V+		


k]	 RI
N ! H

 cE	

s- g z]u




  .  F@
r. V [   	3Jg	6
k

2	 Zg	
x j?

 u9	
 C %Y		a  u
   HDG
\	 - t

q  8it  , ghF
s
  &>  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                         
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              	                                                                                                       
                 	                                                                                                                                                                         	         
                                                                	                                                     	                  	                     
         
                  8   @      	   %   )               -                                                                                                                                                                              
                                                                                                                                                                                                                                                                                                                                                                                        	                                             
       	                                                                 	   
                        	                   	      	       
   
      8 @  	 % )     -   Mz  M  M  N  O  RD  U/! B
0dK  
T  /  4	
gQB

QB
QB
?E|	.-e) S			sY?#_%_
c(dOu _^
	 d? v t
:y8* ? _
q

m
9 3N	
. gVi?*j}
3
SrKg^	. gf\	 0-	
g?  J	U				 $W		
3
h k Ca2	58 (YDY)	 /7f?8O		
 ?L	
LY 39N	z(]niLq% ?  J		@  	V			 9CVjOfp]j
j 47j|`c1	z
*  q


V	
\! 	
 
j `c1	47j|z
 	k

j 47j|`c1	z
G,V]j	
\!> nM\izu

S 3

u	
,8]y 	,AHu	
8w	
v
La[h/q	`DAsTe<B
[.I
PQ9n		7
!q
hp Y.m	HS	gh	]A<seB
HTG,La[hw	
v
	Q  &1
`F	<	
tcE	

s-"	G`& )M	$3/Epgm{"=	
$u 
 ; }	@    - 	K	V	^				"
w* ePq

@V+		


k]\ h	 *  )eM	$3D/EP{pee
q




]g7 J L W \   LUdp
(*Kh02;Vz}<t;
S				7
ZD{X)K,	|

g z@V]j+		?
u








k]  0og@NVM^k+]jq			o



;k]}O v   %[altdw	
 u
(  4<
b
!-]Q%lX
b			

 )tM	$3/E=p	
)gZ\-       <7zXZ  ִF !   t/ ] ˇf{3(1v%f M*	mj)p80*jBJA>f"AvӒp&s{3Th$h|=27|r6
ͽ;29SNx*mq:Mҁ-76)_g+TST~6n]JHbG>7QaM%8.Oc=QEf+	D1Xe)>G쿆K<w:;    =   eѼϱg    YZ  
<7zXZ  ִF !   t/>\	] 3D].?I*ә_.ZvќP,Vj,ު~mC<ґ?vd;!U''yzYl.vU[Qec <L3cGq~=X?r+<ɵؿĊG]1|=ty?izd@PpVvG.	 zuP]-*H)t_hjg½	O{&@T<BohlW~dcXMjʧY@ǎh|_VK\V2B96QQ~ҦTRaTL0N&|#+zwF	纚 yT?j.k=Vk)_s=yy9qhLoDPtYX_>`ǖ$``3]#"k8OKsC6߈Dra}9A_qk@2*RaȂ
 jlQmY,Sdku:@dM܄Wjxn-u3e,W[Uw}kXUJ`=ӅB0eoe/ț	694jutw@lNSQE[ͶQYI <@r'mlZ "̸tL;٠7& fQنNPp
d?6CC@瞼_1Prwzh>[CȖ4 u9>g:j̡T]5Hu[_b:>sI+22ґEiFZṭ^ɴv0&C<R38w8ߛ;3lmW^3{dt/ǈKQ_w{x&./͜-h	'[A?Mw+&/T&H*!Zh%ݾL?5ld>%њ(2,rVAyTZ's/--` B٦VuB 1
i^5T̏f-s>9˱
H&H~çtaI;D'BiݗMA7adߔyK'->Z.>xbyyϚؾr^#O3SSKB΄qڔ"\sڶ"`BqEY̋T4w5C=>\Y렿UsNQG#^Y4	qz}	1|:ދ1}׎/畭fg27 ߢJ馼MdJ<-D7!jlȇ07|W@\] H5"=d)#upϵB3fRZkw~ֵBW7VI<i$+5%ݓw학ĉ W8a#l=<~A8FLu<bϐS-=6|E1Jev~qLٜ&cge={mR@!8}֨XӏYIi?!^;~%:hxbAXD@)+*^J}T]k珬vYF!DJP7O2b}j'	U_xD4yئeIpcwS(GBi"Gu&H/Z='>#rK¡%O<'IEX K*=jˠ؈څ`DGr5 2w>Dx`gY&KM/3Yp<VTˡwj
ld *M'4իzˊ^:[U}8%r1cPعx*csXEMx3
#0jABELCFhl.0&*8!(۽Pk;ṣ@&\Y>	kr$NrGr_
JrDYd(W1Db2Wa"Q*{xX{M\N(DOIzZ="2Sɑ=}~smV	$1b!S"[/1-.>kK\᠌"-^2kyjt]`]{Y<C5nvc.toEA?)ӊU/j@8p(=E(vj8MWfǾ@ %Zr5MEO{Dggu=5&ҍeP Ӱ}E/.BW2e	QyIt^Pzfx=]VM>;X,E R@97V0yX-^K1!v\$P!VSeifyo*1L%>27xW.DJ ZäқםɇtKI}bh#\lYxx/a6mФ*;9 pIQ,66bI_("@\R%wwFs۬ɻùtm~   ^T, |  3߱g    YZ  
   
  P7zXZ  ִF !   t/] ˇf{3(1v%f M*	mj)p80*jBJA>f"AvӒp&s{3Th$h|=27|r6
ͽ;29SNx*mq:Mҁ-76)_g+TST~6nlFҴ22BaK5(hѨkl	>}Z2uqM2{YS.)CBlsMЕ3ݜ<m	S7([    $NQ   Xؐg    YZ  	|7zXZ  ִF !   t/4	:] 3D].?I*ә_.ZvќP,Vj,ު~mC<ґ?vd;!U''yzYl.vU[Qec <L3cGq~=X?r+<ɵؿĊG]1|=ty?izd@Ppw[ږ	##DCU1jqtyb`> ]xBY!kB_2+o+#ʷqTJOͧ>_RIϜҳvP&a-˹*M!<[G¸*WݚÃБWW_F%D)x݈,lNn @l24;yeM;ؗ4ӄNJ6fqnn6X!Y}5!5l?׀dbN)Зv0hO}KKxrxixXzZགྷaC	DxI sO(䏖PKv=B%xGl67/TZЕ=aWE|A!W%lUv	=-|w: 9a$[A(Ï}7kGԡ>AZƾe`%v-'w-haQt>RW$(Z28_pel{5V;Z[8kQ2CҼ{b3P
Ձ^21wѱGڠz`Św/auռy-D'QL[
+\ۇcMWV-s$FLVgGg5K)J'dG3֜0g%ǧE^5Ersxm%2f?$e: /M7USd-m{38(XG~D#p?M0IiI9"ߑhuXSஊbZr\{uIr?S%0Uo:HX\K*Ƨjnv~tűsjp"sP+m0#,/R_ܱ+<#*(*-2_دӮly{w0$|H`قGU%dsg|bWW}`.)!A_'Dבp[׌ hgCٔiխ A9qR*ŸGnApϣϧ9BΐUskS~Zq59a	yP?"eWh{smOêsC)	F)j'$AU'jɚ69:2<~QA}FRYV
ULpRbNZx+h
S<g+fSK4`NP`a#w|5 _Q,YQy$*H\\U  Āܟ	C**fz%~#^69rHH!#[no:##QK(y82)6\db:YZRi6ѭgRÕÀ>"j'\ZzkjPEt-RICNG=u8u/ϜXSALm51`dϕkl4LĞÒd;ֈG쎖H1v=U}jwrj_^U[(u[T-^%!0Ǜe"wqrî3hor.L\>XY=Q~-]^-j&<.Gu#s?Q~mn#%'11'4f(߽$=h|u+dVw#QlIצV=;i~^aȿz e["
SkMOD-sI?>`lD)?:{Sl9sT5Eއِlt"w$vʰ'Fo$/%<@$tx AemL`pp	꜄ũEwX7aϥ:_6I{ى_vBM`/u;CMQZ抦k4gEr'S<F?<wV-|^<4)TJB[F:Q)L3_2[-'-?ӮC8RnWv*}ǭ*(f ⨠?ʍ44{ձV-7ԡꀢJ-iOW(ƍ{Xg&{q:]u/,dPEK$#><,ilF>ҜrxIQ˶   ſH i  vrg    YZ  	   	  ,7zXZ  ִF !   t/] ˇf{3(1v%f M*	mj)p80*jBJA>f"AvӒp&s{3Th$h|=27|r6
ͽ;29SNx*mq:Mҁ-76)_g+TST~6nlFҴ22B^&x$M) 0-mpV>4pzD*+U+S0PYGDKCSN%"!¸m+{~JL]Oz Ldh#%\^{%ܳ;GbјxSw Fɜ4T4C"f޹vrK]L׀} &Pˡsvk3`Ha!
;~]v `aBPecsc	OY0rH_ASOtңt%?4;   

`
 	  Hg    YZ  l7zXZ  ִF !   t/$+] 3D].?I*ә_.ZvќP,Vj,ު~mC<ґ?vd;!U''yzYl.vU[Qec <L3cGq~=X?r+<ɵؿĊG]1|=ty?izd@Pp&Pbmg|a^5؈ZS,AHkz`ȪJGmAM=XLK%7sjL.e3u\,a)2zՈs^yWd%q {eoZ$Ŝ	U\1mqxhD{}oL/RY}'Wlm4(CevMQAB@%VW˙Qb1W8/aq}dKlsr"Oq&C)뉁ea\a$b'pY̺ N@ZXNa g#CP-3rH y_^pq'$9Ӥwv&sycoarm`G]'Ø;[iSQhƭH{Xyh4eӨu3d%rN)zemBdq|PQ?8eY [vj6<Rnx/,A4<J)>*=bݳ6MYY|(8=[2T̜ەvНmtLـ:\rέ+QkU:Oܚ EI8-1	k7͈II#BS<TwbWqN 
BKA0AɁI OtS/V1\8IA]q&Я-5TGZ}rh{qB܃:?)<>?*M$j	]rdg"t[>XDZaoH-xen	Mw͛JBqyճau1 dK#%	j6@y:Nu\ 8DgI~Ȼ֙]1a 7rn5>E!緓	^Sߢ\
&ӖW6ד{ېw?zR&@
J8Xai k"Lvo;zK}YTO'->DT?bS"/cbc WΣ%.qGɻU0T8g;5w+j8n/hg>O(a L\s[Qk/h&,<lc:>nWo踞h/h'Nȳ;gSR/#x]DdH?̶%JE=+--Wït߂NR1"\07$PD4!>/i^Z8ˑb9qh#XqEmm,~k1yv'E?̵E$Olq*? +~u!LnxB!Ϛ.v@=@FokJްc)z/.`jOt-frmЈR$ov[A>/c>	
&ꏣ׼ڪZ/9Πg[ KW`Y%<˹Q'oWZ]š:c:{\u
a@wkÕ4HtucٔJt䇰tuRu-oڪB_.Ᏺ6}BMƆ~#`Q3Ip  g I  c#g    YZ       H7zXZ  ִF !   t/	] ˇf{3(1v%f M*	mj)p80*jBJA>f"AvӒp&s{3Th$h|=27|r6
ͽ;29SNx*mq:Mҁ-76)_g+TST~6nlFҴ22B^&x$M) 0-kqS{97r@E v	C˚ّ|th#{o7;L" (    <l S   ~g    YZ  P7zXZ  ִF !   t/"] 3D].?I*ә_.ZvќP,Vj,ު~mC<ґ?vd;!U''yzYl.vU[Qec <L3cGq~=X?r+<ɵؿĊG]1|=ty?izd@Ppu/bIRKƌbn.txoB+NM6_oa FT#7Vdx'2z
w DjBDGۺG60 2đ%4^ᇐ-`dKJ3\	ĵ%݅S/(~2.8*?g!n1/) ү
򕀙ėIZtkcZLOJD#<*Qd86p0˛[P+c)W6ߝgcWH>j\<!<?w`eEĨyvؖ	tNt$h)˅RzGoE؋O&]!DAHJ9g4(?$G~bG=?4KZjз22Ԇ	)IqUܼV)ܹm:nӬ+\yb&RLAG4Id'5^S
Ԣ桶=*bJ*WГ9XGno~@KIdvL k74˕Jp_	H.RQЧw2Uj:hg̸, Jb\
ƐHj~CV8PX~ObctN٘&37
 -	=Fs,dXq52rs&`*
"bQi
fdm2?̈ø&[:Ν̰dq#Qz(IKk)99DVCYjfi_\NZj20px?̀_,+V;7'ьeCw^z^fBAw象ү׿x!iS6ZP~'}dYd]=޴w__ʠb'|Xuz,#P#
<)J+%ꍷ^DT9)+zAX5J%5KjAj_N\>)dz@SeByT6L%m6ɷV3RI$mQ W%30NDy[  ]*#&JY ;T	kr/vN=c^<4k'IZ^o-.Qo=hN=bBWY)L!ʣh If}s"tΨqǽM?hKZA[<x杧eYU;:	!(7{.0++}TޕkC=s
g9c;nXezq|Hw9J;?^%F-aəDjb uVuAõfzT䓱GytȈG`#y%ဇf;|.O UPl7`@v2r*~Ez42Bf8Ul0&e!9JpĮh]M̙D),7t	eb 5y\aSR/L:*Hе \WxGa|݁`pk:n{yq5.GHUr%Kċs  ~p	r E  b=g    YZ          
         
9 O{y.Ee*^نF̦`Uc*W5ߥe!g=5ViK_pq%R2 '[`,ʘ]+L^&͛H1ڿOl&ůqt採L0oZIBAiB lo9uOAN=^M>p.7,M;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ.9Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^jFUAߑZUaJmnTI^ҟXm!Ln.j({$e@HXgG;|;ѩW@3E 5o>NDҖJ(9 sʂ`kNPħa(UL);(RR"{`7cF:!ԶE&0ģ?Y=4^R;mU ^A|oJ%xn۪z*!5WACU5UeXg{hSjm.L٨XUՐ
7j  7zXZ  ִF !   t/6] 3D].?I*ә_.ZvќP,Vj,ު~mC<ґ?vd;!U''yzYl.vU[Qec <L3cGq~=X?r+<ɵؿĊG]1|=ty?izd@PpS- 
bC+ h'it
^'
h&S.E.٢/ZǼ9Apx0|Н4	OU?kzr-D2Jy~P>vk237;,2Ox1ĚV¿fGU~HTC(ܓbt+ڪgA%R..dΫٹa%w{G.ɽ1MVC<޵){χ~JG,
1k0S L(6Uu@'q};d{@R/KvRr-0of,ðm&@)nyd"9xGpk<sN$~Ifcy!6	_^-SA[^{@<6>.@QoϬ_|u)@/~&r?H=tFF@W2ƃ^*Ūˮ¨T@7;k&?nԘI륺+_# [H`*DhIfD&SvcfDBc"j]=45.*"Y%܀d9ߛ7W41+6-<:*40{:?_Zrd-Z\0KLƜm{8}MM,&9H뢜L<ӌ>i{Lfq_Fx_qU0Ȗ =3RK prɫQՉ-%1]ܪkEm{>ς?{-@Iϑ8-s'#W¯T*zsv{4 {΀܋7SP$<D;CMD8sGR&c>HP:+Z'׾4v$j?GZN	l:;G@efJX(ТS)9Hw9!rW	7U_QA p&k.a0xQ.Jǫ {.s]+H4
Xy*[V p XM3Xdon?OKވ#WS5Hޔ΀[|)Rot
F*('
6)l=Ţį!Z*Z85_̽ekX[gܯIɷG^^n`O9ME{	6yrjqBH,)3`̥dC    # (  %g    YZ                  9=UGC@)@o֍8e|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_90l   D   I   R   T   ~    7zXZ  ִF !   t/t] 3D].?I*ә_.ZvќP,Vj,ު~mC<ґ8hlFOlY.k(DW`!
 	KJ9rEPܤ$FcprMUq3+Xfg=Gj6F#5Ɇca>48r7Nn2h݊r3u>ɧ&[_ 9o)b`.5·QhxOAd)9"qOG`yPlqsLu	j3^񋘢UnMM]=*a/aY P Vc(8Z/tMA-3"Ietih7eewH2Uj" V򋷹bg*xV<e﷍iYL bw&r,<K?G-#3iU
)K WqijcR.Z-уVtڡgۖXj%üx"]|pF+epbm<Ө7#<=5­ְ~zAw7pᙇ⭷5-oq2iM:%9*ݬOqcX8rjhWҟGs\/&Gݡ/x:lSQƈS7ĕӟrz<3V(9yiVpjfIWZॗ-KǗܹ~(M*wixɻ41Goy6@QAp>WeFzʅy[fHCtFXEV_[yuG"|+`V\q0Nw
z>Fg%jlzae.hSUɃBGR+nDsL'wmc}u)4sgT_|~tr1#K$$XX֝\!ɲLr.rw
Oإ(<ɞ!M	/	`P2&jμNi8y{N.-o]7bXY]"'Db.XdZX$Uۋo{giOE|kXF}hsdkcuKJ.a%J=}'l~z+{PUX +jrzU -	5" Ա80wU˨ePvZ4};yr{p{%
%[uctIU#(W	^W?_0x{/]w`[MIB LIolY.W5R]3"$p@^$!?& )ʲa:lê>$dp2d]$Sfv{y7-BkV  /8 /  qWg    YZ                  9=UGC@)@o֍8e|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    D   I   R   T   ~    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="81-87" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="15-62" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="108-126" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="125-125" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-612" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="163-612" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="163-612" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-253" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="314-334" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-127" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="613-662" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="233-252" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="233-252" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="233-252" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="233-252" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="313-333" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="313-333" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="313-333" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="313-333" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:47:54">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  Uʑ 9Z4b	gdYUIUVZSmlЩ18{);NFxӞxMN8nS׍TY^eYT׉gYeF`E
#gS֞RcQqz^%Ҕ%$RNXQIl놵fZJUU;   ;   @   I   K   u    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-78" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="99-117" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="116-116" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="118-603" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="154-603" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="154-603" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="225-244" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="305-325" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="118-118" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="604-653" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="224-243" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="224-243" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="224-243" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="224-243" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="304-324" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="304-324" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="304-324" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="304-324" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:47:10">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  Uʑ 9Z4ᙋ'륜{yf!fVA&aUYiNꛩB@ cU:ӕ;GNy58㉹O^5RRezAeS^&gMePR%uC+aNZyIEUxJSP^	H9bJuE%VjZ՚j*UU`C$   ;   @   I   J   t    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-77" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-116" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-115" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="117-602" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="153-602" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="153-602" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="224-243" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="304-324" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="117-117" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="603-652" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="223-242" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="223-242" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="223-242" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="223-242" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="303-323" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="303-323" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="303-323" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="303-323" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:33">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  Uʑ 9Z4ᘱ|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   r    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-75" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="96-114" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-113" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-600" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="151-600" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="151-600" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="222-241" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="302-322" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-115" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-650" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="221-240" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="221-240" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="221-240" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="221-240" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="301-321" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="301-321" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="301-321" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="301-321" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:28">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  Uʑ 9Z4|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   n    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  X<?xml version="1.0"?><Features recycledIDs="10,11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="92-110" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="109-109" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="111-596" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="147-596" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="147-596" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="218-237" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="298-318" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="111-111" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="597-646" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="217-236" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="217-236" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="217-236" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="217-236" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="297-317" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="297-317" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="297-317" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="297-317" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:14">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  13     Q          Uʑ 9Z4JHVYaUjUgꩶTZjzbފj*E($4Ji{Yjߩ%Qj֔e-ehijbD%&aaeھZ&QOdI}VƄ냦ZQje:][)}TzzAJFe؋G&MjfrީiJ(IVY:fS>U*.JQ%IEDnվ$Z&m:th`a&[H)RݴvU*i4ednjqKinud=mYVM;RUVU^$VV: ɪiEUTf۔ꚕ9F
/T -h{Ev	YkTY*=`UnUNmYe
dKmT<떪aR%)f(gUJjC%4flCNZ*yJIGmj@NZWJZZ1.JYeknb[sYj>Rnj(IibZYfNijM^EBڙzJ^Zb虩[Jf9v^ɤ|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   G  
/  
j    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="21"><Feature recentID="20" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="122-2608" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="19" name="FTL_0126" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="122-2584" color="#00e600" type="standard"/></Feature><Feature recentID="18" name="hypothetical protein" directionality="2" translationMW="95341.69" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="122-2584" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2608-2608" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="16" name="pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="122-2584" color="#a6acb3" type="standard"/></Feature><Feature recentID="15" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2609-2627" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2610-2610" color="#a6acb3" type="standard"/></Feature><Feature recentID="13" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-121" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="12" name="FTL_0125" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="72-117" color="#00e600" type="standard"/></Feature><Feature recentID="11" name="hypothetical protein" directionality="2" translationMW="1785.21" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="28" maxFusedRunOn="28"><Segment range="72-117" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="pdpB" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-117" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2666" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2665-2665" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2667-3152" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2703-3152" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2703-3152" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2774-2793" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="2854-2874" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2667-2667" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3153-3202" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P823 DFPI_FR1R2" sequence="atatGCGGCCGCtgctatcattgtctacttaattagaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2574-2602" boundStrand="0" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="2574-2602" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite><BindingSite simplified="1" location="2574-2602" boundStrand="0" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="2574-2602" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite></Primer><Primer recentID="1" name="KROL311  pdpA_qPCR_R1" sequence="cacttgatctttgtatcgcactatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2133-2157" boundStrand="0" annealedBases="cacttgatctttgtatcgcactatc" meltingTemperature="57"><Component hybridizedRange="2133-2157" bases="cacttgatctttgtatcgcactatc"/></BindingSite><BindingSite simplified="1" location="2133-2157" boundStrand="0" annealedBases="cacttgatctttgtatcgcactatc" meltingTemperature="57"><Component hybridizedRange="2133-2157" bases="cacttgatctttgtatcgcactatc"/></BindingSite></Primer><Primer recentID="2" name="KROL310  pdpA_qPCR_F1" sequence="cttgagctccctcatgattaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2190-2211" boundStrand="1" annealedBases="cttgagctccctcatgattaca" meltingTemperature="57"><Component hybridizedRange="2190-2211" bases="cttgagctccctcatgattaca"/></BindingSite><BindingSite simplified="1" location="2190-2211" boundStrand="1" annealedBases="cttgagctccctcatgattaca" meltingTemperature="57"><Component hybridizedRange="2190-2211" bases="cttgagctccctcatgattaca"/></BindingSite></Primer><Primer recentID="3" name="KROL312  pdpA_qPCR_F2" sequence="aatcatgagggagctcaagaatta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2193-2216" boundStrand="0" annealedBases="aatcatgagggagctcaagaatta" meltingTemperature="55"><Component hybridizedRange="2193-2216" bases="aatcatgagggagctcaagaatta"/></BindingSite><BindingSite simplified="1" location="2193-2216" boundStrand="0" annealedBases="aatcatgagggagctcaagaatta" meltingTemperature="55"><Component hybridizedRange="2193-2216" bases="aatcatgagggagctcaagaatta"/></BindingSite></Primer><Primer recentID="4" name="KROL313  pdpA_qPCR_R2" sequence="cgacaaactataaagactct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2269-2288" boundStrand="1" annealedBases="cgacaaactataaagactct" meltingTemperature="50"><Component hybridizedRange="2269-2288" bases="cgacaaactataaagactct"/></BindingSite><BindingSite simplified="1" location="2269-2288" boundStrand="1" annealedBases="cgacaaactataaagactct" meltingTemperature="50"><Component hybridizedRange="2269-2288" bases="cgacaaactataaagactct"/></BindingSite></Primer><Primer recentID="5" name="KROL314  pdpA_qPCR_F3" sequence="acccaatatatcgtgaaaggct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2067-2088" boundStrand="0" annealedBases="acccaatatatcgtgaaaggct" meltingTemperature="56"><Component hybridizedRange="2067-2088" bases="acccaatatatcgtgaaaggct"/></BindingSite><BindingSite simplified="1" location="2067-2088" boundStrand="0" annealedBases="acccaatatatcgtgaaaggct" meltingTemperature="56"><Component hybridizedRange="2067-2088" bases="acccaatatatcgtgaaaggct"/></BindingSite></Primer><Primer recentID="6" name="KROL315  pdpA_qPCR_R3" sequence="gatagtgcgatacaaagatcaagtg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2133-2157" boundStrand="1" annealedBases="gatagtgcgatacaaagatcaagtg" meltingTemperature="56"><Component hybridizedRange="2133-2157" bases="gatagtgcgatacaaagatcaagtg"/></BindingSite><BindingSite simplified="1" location="2133-2157" boundStrand="1" annealedBases="gatagtgcgatacaaagatcaagtg" meltingTemperature="56"><Component hybridizedRange="2133-2157" bases="gatagtgcgatacaaagatcaagtg"/></BindingSite></Primer><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="2773-2792" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="2773-2792" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="2773-2792" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="2773-2792" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="2853-2873" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="2853-2873" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="2853-2873" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="2853-2873" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:44:19">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  7zXZ  ִF !   t/l] ˇf{3(1v%f M*	mj)p80*i	NY]QF7&!n%W "*ϻFFS
kagD	0z=.]|MݐHG"] gmZqBכ#ڪ*@>MB>֧H|?w	AawG0:8k1HvXvH}lDح3$#;.W%G4>9e5/P @lRԸ\S+5V</V l\W_K~"뙀F˒5q9f]H3U!ܝ.C]:,GËEIL;;ƮpXCd:.oht4KV3X:<0pT>}i2ks')ktaL20 g*q|52 BrojMU.l:r.h%W=6DzO[LxZM[*+249_fair>Y,PϽ)L;ȒZ
-';e*SFR[8CЊ8&ih9j<&:Fi|au.$L^.Є{5|MpsuNgtK6ղ jwthG0}"P2kc{."lcy@/9kXedHbk'4Bޜ2*F9Q1_%̑|m7ޕHBHA[&|`NUTƫT=:w!)z+/z~tU9jUaAm{9l8,˚ukJCFsb}݇Z\+tV砈qj[tn {-"T椦
'H~UzS,MưmT˖L"E02J/Nua-(.6:`ODMᕛjېb*= 87Þ&55h}T sGq%z>y^ЁGce=_l,zrP)z$ĉFz@r|VH\ƽ(XJGR4Bs'}x!a".	\t !lY?KNSH`iP3ʵ&^,JQׯȜ R{&A(R?/0TWm6@{+ٺ76>olD35L
[	UNfźaX
5g_$X|i2P]fp<kNA(dgRׇS<_|@.6 K¤П1tFΎtzhdOƬItK\n|*mS)'Cɍ5(8X4ranNw̃8e՜vƝ^&-&TmjzH8.@AYuz
T89"W]G6eP\<.)CjQGbڨO=mk2"rxF|˙q\Q:b+@=!R9n' Z/rlD踹yR{/cgU#vHk|2J-aV%mp'sprbXuhb0hL0durvs*~HLaK۰>>%	x-um;=`h6i4aq}C,}y;,ڍHgǐKs3waL*:!bcQx:{$T-Ux.g/JsJ-KR>^9zkHؗ]hK9Bh%OM#0+_L>/؎^TZZt#36vh~W7EeR#TyIY95e#올_{ CS+{({42[z'.R<Z'4ď$o1TRB ݈͟xVԖ@z@J>"b/sIVodSOY^!h$s,ěq]?Gv9[<ӌ؛Ĉr]MPe# -$ 
V KyydfiJ9eڔZOtfn/n_'o+5xZ6kaЪYq.Uf{wpT/Z5Q y"bc&gՕx	)4"{e`Ou+.t抐RM|.q{ԇobf@~m7$>.ߪ:v~)	EK=XEFƃzuPx[tzL(<XɣbEy~{qK)%uO[XD"XB-f3B2BE0!:7Jح<8DŠ0ӌ&XĨ(B,Y\.<AY.A=ݢlHd!
Fw>~d?>RJ̙iC&ҕkyna_\ct®3qe]u
'
gq LH[
}.xR@(lHmNuDu@a$׿m4_:
viEfURJVΧtB&`,MJy|!Epq!?I'[wF44tqAp&P"$[rl"_:8tjk27)	n1Hǵb r-&6XAp[<yn3JU߉  ;ZHS3}7АaRИ      !  icLg    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="18"><Feature recentID="0" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="3350" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="2559-3350" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="3351-3419" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="1" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1799-2387" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3420-3524" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="T7 terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1534-1581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="897-897" color="#a6acb3" type="standard"/></Feature><Feature recentID="5" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="1084-1104" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="6" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1004-1023" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="7" name="lipoprotein" directionality="1" translationMW="15771.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="933-1382" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MKKIIKLSLLSLSIAGLASCSTLGLGGSDDAKASAKDTAAAQTATTEQAAAVSKPTAKVSLNKLGQDKIKATVYTAYNNNPQGSVRLQWQAPEGSKCHDTSFPITKYAEKNDKTWATVTVKQGNNFCSGKWTANVVYDKEVIASDSINI*"/></Q></Feature><Feature recentID="8" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="933-1382" color="#00e600" type="standard"/></Feature><Feature recentID="9" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="895-895" color="#a6acb3" type="standard"/></Feature><Feature recentID="10" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="878-896" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="16-63" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="851-857" color="#a6acb3" type="standard"/></Feature><Feature recentID="13" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="827-827" color="#a6acb3" type="standard"/></Feature><Feature recentID="14" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="826-844" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="superfolder GFP" directionality="2" translationMW="26880.39" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="0" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="70-783" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Segment name="1a" range="784-786" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="787-789" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;GFP variant that folds robustly even when fused to poorly folded proteins (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/16369541&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/16369541&quot;&gt;Pédelacq et al., 2006&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK*"/></Q></Feature><Feature recentID="16" name="Possible TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="825-825" color="#a6acb3" type="standard"/></Feature><Feature recentID="17" name="tul4 UTR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="790-825" color="#a6acb3" type="standard"/></Feature></Features>
  #<?xml version="1.0"?><Primers nextValidID="12"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="1003-1022" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="1003-1022" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="1003-1022" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="1003-1022" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="1" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="1083-1103" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="55"><Component hybridizedRange="1083-1103" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="1083-1103" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="55"><Component hybridizedRange="1083-1103" bases="ttgcagttggcttagatacag"/></BindingSite></Primer><Primer recentID="2" name="KROL358  T7_amp_F" sequence="CTAGTGGTGCTAGCCAAAAAACCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-11-12T18:52:01Z"><BindingSite location="1-24" boundStrand="0" annealedBases="CTAGTGGTGCTAGCCAAAAAACCC" meltingTemperature="60"><Component hybridizedRange="1-24" bases="CTAGTGGTGCTAGCCAAAAAACCC"/></BindingSite><BindingSite location="1571-1580" boundStrand="1" annealedBases="CAAAAAACCC" meltingTemperature="29"><Component bases="CTAGTGGTGCTAGC"/><Component hybridizedRange="1571-1580" bases="CAAAAAACCC"/></BindingSite><BindingSite simplified="1" location="1-24" boundStrand="0" annealedBases="CTAGTGGTGCTAGCCAAAAAACCC" meltingTemperature="60"><Component hybridizedRange="1-24" bases="CTAGTGGTGCTAGCCAAAAAACCC"/></BindingSite><BindingSite simplified="1" location="1571-1580" boundStrand="1" annealedBases="CAAAAAACCC" meltingTemperature="29"><Component bases="CTAGTGGTGCTAGC"/><Component hybridizedRange="1571-1580" bases="CAAAAAACCC"/></BindingSite></Primer><Primer recentID="3" name="KROL359  T7_amp_R" sequence="GATCGATCCGCTAGCCTCGAGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-11-12T18:58:35Z"><BindingSite location="1443-1473" boundStrand="1" annealedBases="GATCGATCCGCTAGCCTCGAGC" meltingTemperature="53"><Component hybridizedRange="1467-1473" bases="GATCGAT"/><Component hybridizedRange="1443-1457" bases="CCGCTAGCCTCGAGC"/></BindingSite><BindingSite simplified="1" location="1443-1457" boundStrand="1" annealedBases="CCGCTAGCCTCGAGC" meltingTemperature="53"><Component bases="GATCGAT"/><Component hybridizedRange="1443-1457" bases="CCGCTAGCCTCGAGC"/></BindingSite></Primer><Primer recentID="4" name="KROL363  T7_seq_1" sequence="GTTATTGTCTCATGAGCGGATACATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-11-12T19:32:18Z"><BindingSite location="3457-3482" boundStrand="0" annealedBases="GTTATTGTCTCATGAGCGGATACATA" meltingTemperature="56"><Component hybridizedRange="3457-3482" bases="GTTATTGTCTCATGAGCGGATACATA"/></BindingSite><BindingSite simplified="1" location="3457-3482" boundStrand="0" annealedBases="GTTATTGTCTCATGAGCGGATACATA" meltingTemperature="56"><Component hybridizedRange="3457-3482" bases="GTTATTGTCTCATGAGCGGATACATA"/></BindingSite></Primer><Primer recentID="5" name="KROL364  T7_seq_2" sequence="aacttgggcaactgtaacagttaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-11-12T19:33:45Z"><BindingSite location="1270-1295" boundStrand="0" annealedBases="aacttgggcaactgtaacagttaagc" meltingTemperature="60"><Component hybridizedRange="1270-1295" bases="aacttgggcaactgtaacagttaagc"/></BindingSite><BindingSite simplified="1" location="1270-1295" boundStrand="0" annealedBases="aacttgggcaactgtaacagttaagc" meltingTemperature="60"><Component hybridizedRange="1270-1295" bases="aacttgggcaactgtaacagttaagc"/></BindingSite></Primer><Primer recentID="6" name="KROL402  GFP_PCR_R" sequence="tagtggatccttatttatataattcatcc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2021-01-26T14:30:42Z"><BindingSite location="63-87" boundStrand="0" annealedBases="ggatccttatttatataattcatcc" meltingTemperature="49"><Component bases="tagt"/><Component hybridizedRange="63-87" bases="ggatccttatttatataattcatcc"/></BindingSite><BindingSite simplified="1" location="63-87" boundStrand="0" annealedBases="ggatccttatttatataattcatcc" meltingTemperature="49"><Component bases="tagt"/><Component hybridizedRange="63-87" bases="ggatccttatttatataattcatcc"/></BindingSite></Primer><Primer recentID="7" name="KROL362  GFP_seq_1" sequence="gttgaattagatggtgatgttaatggt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2021-01-26T14:33:50Z"><BindingSite location="714-740" boundStrand="1" annealedBases="gttgaattagatggtgatgttaatggt" meltingTemperature="56"><Component hybridizedRange="714-740" bases="gttgaattagatggtgatgttaatggt"/></BindingSite><BindingSite simplified="1" location="714-740" boundStrand="1" annealedBases="gttgaattagatggtgatgttaatggt" meltingTemperature="56"><Component hybridizedRange="714-740" bases="gttgaattagatggtgatgttaatggt"/></BindingSite></Primer><Primer recentID="8" name="KROL403  GFP_PCR_F2" sequence="aattgAGGAGGTTTATatggtttcaaaaggtgaagaattatttac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2021-01-27T16:12:19Z"><BindingSite location="760-800" boundStrand="1" annealedBases="AGGAGTATatggtttcaaaaggtgaagaattatttac" meltingTemperature="52"><Component bases="aattg"/><Component hybridizedRange="796-800" bases="AGGAG"/><Component bases="GTT"/><Component hybridizedRange="760-791" bases="TATatggtttcaaaaggtgaagaattatttac"/></BindingSite><BindingSite simplified="1" location="760-791" boundStrand="1" annealedBases="TATatggtttcaaaaggtgaagaattatttac" meltingTemperature="52"><Component bases="aattgAGGAGGTT"/><Component hybridizedRange="760-791" bases="TATatggtttcaaaaggtgaagaattatttac"/></BindingSite></Primer><Primer recentID="9" name="KROL407  GFP_PvuII_F" sequence="atatcagctgAGGAGGTTTATatggtttcaaaaggtgaagaattatttac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2021-02-05T19:31:50Z"><BindingSite location="760-800" boundStrand="1" annealedBases="AGGAGTATatggtttcaaaaggtgaagaattatttac" meltingTemperature="52"><Component bases="atatcagctg"/><Component hybridizedRange="796-800" bases="AGGAG"/><Component bases="GTT"/><Component hybridizedRange="760-791" bases="TATatggtttcaaaaggtgaagaattatttac"/></BindingSite><BindingSite simplified="1" location="760-791" boundStrand="1" annealedBases="TATatggtttcaaaaggtgaagaattatttac" meltingTemperature="52"><Component bases="atatcagctgAGGAGGTT"/><Component hybridizedRange="760-791" bases="TATatggtttcaaaaggtgaagaattatttac"/></BindingSite></Primer><Primer recentID="10" name="KROL430  pKR102_seq" sequence="cttaattatttttttcatatagatactcct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2021-04-23T18:19:50Z"><BindingSite location="786-800" boundStrand="0" annealedBases="catatagatactcct" meltingTemperature="39"><Component bases="cttaattattttttt"/><Component hybridizedRange="786-800" bases="catatagatactcct"/></BindingSite><BindingSite location="920-949" boundStrand="1" annealedBases="cttaattatttttttcatatagatactcct" meltingTemperature="50"><Component hybridizedRange="920-949" bases="cttaattatttttttcatatagatactcct"/></BindingSite><BindingSite simplified="1" location="786-800" boundStrand="0" annealedBases="catatagatactcct" meltingTemperature="39"><Component bases="cttaattattttttt"/><Component hybridizedRange="786-800" bases="catatagatactcct"/></BindingSite><BindingSite simplified="1" location="920-949" boundStrand="1" annealedBases="cttaattatttttttcatatagatactcct" meltingTemperature="50"><Component hybridizedRange="920-949" bases="cttaattatttttttcatatagatactcct"/></BindingSite></Primer><Primer recentID="11" name="KROL441  GFP_tul4UTR_T7promoter" sequence="atatCAGCTGTAATACGACTCACTATAGGgagtatatgtgaatatttaaaaataggagtatctatatggtttcaaaaggtgaagaattatttac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2021-05-12T14:42:58Z"><BindingSite location="760-852" boundStrand="1" annealedBases="tatCAGCTGTAATACGACTCACTATAGGgagtatatgtgaatatttaaaaataggagtatctatatggtttcaaaaggtgaagaattatttac" meltingTemperature="66"><Component bases="a"/><Component hybridizedRange="760-852" bases="tatCAGCTGTAATACGACTCACTATAGGgagtatatgtgaatatttaaaaataggagtatctatatggtttcaaaaggtgaagaattatttac"/></BindingSite><BindingSite simplified="1" location="760-852" boundStrand="1" annealedBases="tatCAGCTGTAATACGACTCACTATAGGgagtatatgtgaatatttaaaaataggagtatctatatggtttcaaaaggtgaagaattatttac" meltingTemperature="66"><Component bases="a"/><Component hybridizedRange="760-852" bases="tatCAGCTGTAATACGACTCACTATAGGgagtatatgtgaatatttaaaaataggagtatctatatggtttcaaaaggtgaagaattatttac"/></BindingSite></Primer></Primers>
  d<Notes>
<UUID>29ed4c85-fedb-49ab-8534-eab1650477d4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="19:16:25">2020.11.12</Created>
<LastModified UTC="14:49:9">2022.4.7</LastModified>
<CreatedBy>kramseylab</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         6+ Cutters                                                                                                   d     CHO                E              Y? P                                                                                                                                                              )<?xml version="1.0"?><CustomEnzymeSets/>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
