	   SnapGene      ,>tatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacaatcgatcaagggttggtttgcgcattcacagttctccgcaagaattgattggctccaattcttggagtggtgaatccgttagcgaggtgccgccggcttccattcaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgatctatacccttcaagctttgaaaaataaacttaattattatatatgttatttagctagtttttttaattaaagttaaaatcgagagcttgtttgacaaaaaaacaaaaaaatttcttgaaaattttttttttgactcaatatctagacttgcaagagcttggaactttgagattgttctaagatgcatacaaattcaaaatgcttaaacaaaaataatttaacaaaggagtaagattgttatgagccatattcaacgggaaacgtcttgctcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggaaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataaacttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagaattggttaattggttgtaacactggcagagcattacgctgacttgacgggacggcggctttgttgaataaatcgaacttttgctgagttgaaggatcagatcacgcatcttcccgacaacgcagaccgttccgtggcaaagcaaaagttcaaaatcaccaactggtccacctacaacaaagctctcatcaacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttcatacacggtgcctgactgcgttagcaatttaactgtgataaactaccgcattaaagctaatcggttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagagaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccataatcggcattttcttttgcgtttttatttgttaactgttaattgtccttgttcaaggatgctgtctttgacaacagatgttttcttgcctttgatgttcagcaggaagctaggcgcaaacgttgattgtttgtctgcgtagaatcctctgtttgtcatatagcttgtaatcacgacattgtttcctttcgcttgaggtacagcgaagtgtgagtaagtaaaggttacatcgttaggatcaagatccatttttaacacaaggccagttttgttcagcggcttgtatgggccagttaaagaattagaaacataaccaagcatgtaaatatcgttagacgtaatgccgtcaatcgtcatttttgatccgcgggagtcagtgaacagataccatttgccgttcattttaaagacgttcgcgcgttcaatttcatctgttactgtgttagatgcaatcagcggtttcatcacttttttcagtgtgtaatcatcgtttagctcaatcataccgagagcgccgtttgctaactcagccgtgcgttttttatcgctttgcagaagtttttgactttcttgacggaagaatgatgtgcttttgccatagtatgctttgttaaataaagattcttcgccttggtagccatcttcagttccagtgtttgcttcaaatactaagtatttgtggcctttatcttctacgtagtgaggatctctcagcgtatggttgtcgcctgagctgtagttgccttcatcgatgaactgctgtacattttgatacgtttttccgtcaccgtcaaagattgatttataatcctctacaccgttgatgttcaaagagctgtctgatgctgatacgttaacttgtgcagttgtcagtgtttgtttgccgtaatgtttaccggagaaatcagtgtagaataaacggatttttccgtcagatgtaaatgtggctgaacctgaccattcttgtgtttggtcttttaggatagaatcatttgcatcgaatttgtcgctgtctttaaagacgcggccagcgtttttccagctgtcaatagaagtttcgccgactttttgatagaacatgtaaatcgatgtgtcatccgcatttttaggatctccggctaatgcaaagacgatgtggtagccgtgatagtttgcgacagtgccgtcagcgttttgtaatggccagctgtcccaaacgtccaggccttttgcagaagagatatttttaattgtggacgaatcgaactcaggaacttgatatttttcatttttttgctgttcagggatttgcagcatatcatggcgtgtaatatgggaaatgccgtatgtttccttatatggcttttggttcgtttctttcgcaaacgcttgagttgcgcctcctgccagcagtgcggtagtaaaggttaatactgttgcttgttttgcaaactttttgatgttcatcgttcatgtctccttttttatgtactgtgttagcggtctgcttcttccagccctcctgtttgaagatggcaagttagttacgcacaataaaaaaagacctaaaatatgtaaggggtgacgccaaagtatacactttgccctttacacattttaggtcttgcctgctttatcagtaacaaacccgcgcgatttacttttcgacctcattctattagactctcgtttggattgcaactggtctattttcctcttttgtttgatagaaaatcataaaaggatttgcagactacgggcctaaagaactaaaaaatctatctgtttcttttcattctctgtattttttatagtttctgttgcatgggcataaagttgcctttttaatcacaattcagaaaatatcataatatctcatttcactaaataatagtgaacggcaggtatatgtgatgggttaaaaaggatcgatcctctagctagagtcgatcttcgccagcagggcgaggatcgtggcatcaccgaaccgcgccgtgcgcgggtcgtcggtgagccagagtttcagcaggccgcccaggcggcccaggtcgccattgatgcgggccagctcgcggacgtgctcatagtccacgacgcccgtgattttgtagccctggccgacggccagcaggtaggccgacaggctcatgccggctgccgccgccttttcctcaatcgctcttcgttcgtctggaaggcagtacaccttgataggtgggctgcccttcctggttggcttggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccagcctcgcagagcaggattcccgttgagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaaggatggatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaagcgctgcttccctgctgttttgtggaatatctaccgactggaaacaggcaaatgcaggaaattactgaactgaggggacaggcgagagacgatgccaaagagctacaccgacgagctggccgagtgggttgaatcccgcgcggccaagaagcgccggcgtgatgaggctgcggttgcgttcctggcggtgagggcggatgtcgatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgcatatttgaatgtatttagaaaaataaacaaaagagtttgtagaaacgcaaaaaggccatccgtcaggatggccttctgcttaatttgatgcctggcagtttatggcgggcgtcctgcccgccaccctccgggccgttgcttcgcaacgttcaaatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgccgccaggcaaattctgttttatcagaccgcttctgcgttctgatttaatctgtatcaggctgaaaatcttctctcatccgccaaaacagccaagctcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgccaagcttgcatgcctgcaggtcgactctagaggatcctcataatcaaagtcaacaaatgaaaacgtttctttactcggtttaaaagtaattagtgttccggtcttatctgttgaaccaacttcttttaatggaaactgtggtactccatgaacatactcttgatagtgctcgataccgtttctataaattgttaactttaattgttctgaaagcgcatttacaactgatacaccaacaccatgtaaaccgcctgatactttataagagttatcatcaaacttaccacctgcatgcaatactgtcaaaataacctctgctgcagagcgcccttcttctttatgaatatctgtaggaatacctctaccattatctgatacagatacagagccatccttatttataataactttaatatcatcacaataaccagctaaggcttcatcgatagcattatcaacaacctcaaataccatatgatgtaagccgctaccatcatcggtatcaccaatatacattccaggtctttttctaacagcatctaaacctttaagtaccttaatactcgatgagtcataagctttattctcagacattaacaaactccaaatttatcgttatattttaaaattcaatcattaagtattataacacaaataaatcttgattattaatgttgataacaatgtgagttaataaacattgagggtttttcattttatccaaaaatgctaatattaactctaacagttttgcagataggttcattttgaaattttgaaaaaggcggccgcCTTAACTTTATAAGGAGGAACAGCCATGGCCATGATTACGGATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCTTTGCCTGGTTTCCGGCACCAGAAGCGGTGCCGGAAAGCTGGCTGGAGTGCGATCTTCCTGAGGCCGATACTGTCGTCGTCCCCTCAAACTGGCAGATGCACGGTTACGATGCGCCCATCTACACCAACGTGACCTATCCCATTACGGTCAATCCGCCGTTTGTTCCCACGGAGAATCCGACGGGTTGTTACTCGCTCACATTTAATGTTGATGAAAGCTGGCTACAGGAAGGCCAGACGCGAATTATTTTTGATGGCGTTAACTCGGCGTTTCATCTGTGGTGCAACGGGCGCTGGGTCGGTTACGGCCAGGACAGTCGTTTGCCGTCTGAATTTGACCTGAGCGCATTTTTACGCGCCGGAGAAAACCGCCTCGCGGTGATGGTGCTGCGCTGGAGTGACGGCAGTTATCTGGAAGATCAGGATATGTGGCGGATGAGCGGCATTTTCCGTGACGTCTCGTTGCTGCATAAACCGACTACACAAATCAGCGATTTCCATGTTGCCACTCGCTTTAATGATGATTTCAGCCGCGCTGTACTGGAGGCTGAAGTTCAGATGTGCGGCGAGTTGCGTGACTACCTACGGGTAACAGTTTCTTTATGGCAGGGTGAAACGCAGGTCGCCAGCGGCACCGCGCCTTTCGGCGGTGAAATTATCGATGAGCGTGGTGGTTATGCCGATCGCGTCACACTACGTCTGAACGTCGAAAACCCGAAACTGTGGAGCGCCGAAATCCCGAATCTCTATCGTGCGGTGGTTGAACTGCACACCGCCGACGGCACGCTGATTGAAGCAGAAGCCTGCGATGTCGGTTTCCGCGAGGTGCGGATTGAAAATGGTCTGCTGCTGCTGAACGGCAAGCCGTTGCTGATTCGAGGCGTTAACCGTCACGAGCATCATCCTCTGCATGGTCAGGTCATGGATGAGCAGACGATGGTGCAGGATATCCTGCTGATGAAGCAGAACAACTTTAACGCCGTGCGCTGTTCGCATTATCCGAACCATCCGCTGTGGTACACGCTGTGCGACCGCTACGGCCTGTATGTGGTGGATGAAGCCAATATTGAAACCCACGGCATGGTGCCAATGAATCGTCTGACCGATGATCCGCGCTGGCTACCGGCGATGAGCGAACGCGTAACGCGAATGGTGCAGCGCGATCGTAATCACCCGAGTGTGATCATCTGGTCGCTGGGGAATGAATCAGGCCACGGCGCTAATCACGACGCGCTGTATCGCTGGATCAAATCTGTCGATCCTTCCCGCCCGGTGCAGTATGAAGGCGGCGGAGCCGACACCACGGCCACCGATATTATTTGCCCGATGTACGCGCGCGTGGATGAAGACCAGCCCTTCCCGGCTGTGCCGAAATGGTCCATCAAAAAATGGCTTTCGCTACCTGGAGAGACGCGCCCGCTGATCCTTTGCGAATACGCCCACGCGATGGGTAACAGTCTTGGCGGTTTCGCTAAATACTGGCAGGCGTTTCGTCAGTATCCCCGTTTACAGGGCGGCTTCGTCTGGGACTGGGTGGATCAGTCGCTGATTAAATATGATGAAAACGGCAACCCGTGGTCGGCTTACGGCGGTGATTTTGGCGATACGCCGAACGATCGCCAGTTCTGTATGAACGGTCTGGTCTTTGCCGACCGCACGCCGCATCCAGCGCTGACGGAAGCAAAACACCAGCAGCAGTTTTTCCAGTTCCGTTTATCCGGGCAAACCATCGAAGTGACCAGCGAATACCTGTTCCGTCATAGCGATAACGAGCTCCTGCACTGGATGGTGGCGCTGGATGGTAAGCCGCTGGCAAGCGGTGAAGTGCCTCTGGATGTCGCTCCACAAGGTAAACAGTTGATTGAACTGCCTGAACTACCGCAGCCGGAGAGCGCCGGGCAACTCTGGCTCACAGTACGCGTAGTGCAACCGAACGCGACCGCATGGTCAGAAGCCGGGCACATCAGCGCCTGGCAGCAGTGGCGTCTGGCGGAAAACCTCAGTGTGACGCTCCCCGCCGCGTCCCACGCCATCCCGCATCTGACCACCAGCGAAATGGATTTTTGCATCGAGCTGGGTAATAAGCGTTGGCAATTTAACCGCCAGTCAGGCTTTCTTTCACAGATGTGGATTGGCGATAAAAAACAACTGCTGACGCCGCTGCGCGATCAGTTCACCCGTGCACCGCTGGATAACGACATTGGCGTAAGTGAAGCGACCCGCATTGACCCTAACGCCTGGGTCGAACGCTGGAAGGCGGCGGGCCATTACCAGGCCGAAGCAGCGTTGTTGCAGTGCACGGCAGATACACTTGCTGATGCGGTGCTGATTACGACCGCTCACGCGTGGCAGCATCAGGGGAAAACCTTATTTATCAGCCGGAAAACCTACCGGATTGATGGTAGTGGTCAAATGGCGATTACCGTTGATGTTGAAGTGGCGAGCGATACACCGCATCCGGCGCGGATTGGCCTGAACTGCCAGCTGGCGCAGGTAGCAGAGCGGGTAAACTGGCTCGGATTAGGGCCGCAAGAAAACTATCCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAACGGTCTGCGCTGCGGGACGCGCGAATTGAATTATGGCCCACACCAGTGGCGCGGCGACTTCCAGTTCAACATCAGCCGCTACAGTCAACAGCAACTGATGGAAACCAGCCATCGCCATCTGCTGCACGCGGAAGAAGGCACATGGCTGAATATCGACGGTTTCCATATGGGGATTGGTGGCGACGACTCCTGGAGCCCGTCAGTATCGGCGGAATTCCAGCTGAGCGCCGGTCGCTACCATTACCAGTTGGTCTGGTGTCAAAAATAAGCggccgctcctttataattttataacaacttaaatctcgtagcttttaaaatgaaaataacaactagaaaaatttgtatagcaccaatgcttgactggactgataggcactatcgttacatgatgcgtctaatcaccaggcatacaatgttatatactgaaatggttactttaaacgcaatcttacatggtaataaagaatttttgcttaaatataatcctcaagaaaatcctgtgacactacagttaggtggttgcgttccaaaagactttatcagttgtggcaagcttgccgaagaacttgactataatgaaattaacataaatgttggctgcccaagtgaaagagttaaaaaaggtaattttggcttatcattaatggcagagccagagcttgtagcagactgtgtcaaagctatgaaagataacctaaatatcccaatttcagttaaatgtcgtattggctatgatcataatgatagctatgaagaactatataattttgttgataagcaagttcaagcaggaatagattacctttgtattcatgcacgtaaaggttggcttagcggtctatcaccaaaagaaaatcgtaccatacctgagcttaaatatgatgttgtctacagtatcaggtaccgagctcgaattcgtaatcatgtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgacccgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaac  9P  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                 >                                                           
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   +             	   &       '     	 8 I C  / @ 	 * 3 ' &    2   2   B  !  J-  K  L  L;  Q  Y  e
,#9+:!u I	*r)*":!y!!s$5***+"- #&/1 !G#6&G#|(#q
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I4"7"Q# kOR#T&&p)dn&
t1&k((o

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s]		(

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FP  P""#=#$$4%&)+5,gFg'			!^H(31#%j&a''){)*2 s ~   '<AJRlRq+PT/7Rj			
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6X058xq:m&G  "##$$$X&)%*,   %?-P]VLU^|v		
} N/z&KS@T]rFPTwN%IYa NQ   !!"3"B"P"e"o""""#=#L###W$y$$$4%B%K%%&&F&&&&&r''m(B))))** ***#+g+t+++++5, * 7 >   %1?-)Rd%U^}		
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LU^FPbx0ipz@J#]!Ru|#6?.I> E R t   !a!e!!!!"P""""""##=#M#]###&$X$d$z$$$$4%O%U%i%%&&&I's'(b)))***+W+h+u++,5,6|4Y
_Bp}((#%%&&1''$(R(((q))))T<	P8XI""h#'d*+M 7]oP	94tbC*F+X}*9y	L


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ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAmRVrT+鸡}zx{]d|Z)zS6^]%|gJmz}xY~?YYigFtOoYzmY}9CiYaO9	gkкM_IYa-kvWZ)ZRiuenXX*oUci{JFBVUC;eeץn:XU^EVdXxv˖CgXg`\%:VeU!t +GN^aZ]?RT%i`=M"9F_JV,aXd^fZSR4Fܕ
}TU}Y(m	;[b8!Z)ֽvq<R%O#億Z2Wial=s0C
;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%         !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ;S<?xml version="1.0"?><Features nextValidID="44"><Feature recentID="43" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7917-288" color="#ffff00" type="standard"/><Q name="label"><V text="ori"/></Q><Q name="note"><V text="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="42" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7608-7629" color="#31849b" type="standard"/><Q name="label"><V text="CAP binding site"/></Q><Q name="note"><V text="CAP binding activates transcription in the presence&#10;of cAMP."/></Q><Q name="bound_moiety"><V text="E. coli catabolite activator protein"/></Q></Feature><Feature recentID="41" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7563-7593" color="#ffffff" type="standard"/><Q name="label"><V text="lac promoter"/></Q><Q name="note"><V text="promoter for the E. coli lac operon"/></Q></Feature><Feature recentID="40" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7548-7567" color="#a020f0" type="standard"/><Q name="label"><V text="P022 pEX18SeqR1"/></Q><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="39" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7539-7555" color="#31849b" type="standard"/><Q name="label"><V text="lac operator"/></Q><Q name="note"><V text="The lac repressor binds to the lac operator to&#10;inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."/></Q><Q name="bound_moiety"><V text="lac repressor encoded by lacI"/></Q></Feature><Feature recentID="38" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7526-7551" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="37" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7515-7531" color="#a020f0" type="standard"/><Q name="label"><V text="M13 rev"/></Q><Q name="note"><V text="common sequencing primer, one of multiple similar variants"/></Q></Feature><Feature recentID="36" name="FTL_0404" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7489-7493" color="#a6acb3" type="standard"/><Q name="label"><V text="FTL_0404"/></Q><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="3952331" predef="GeneID"/></Q></Feature><Feature recentID="35" name="P792 DFTL_1548FR2R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7461-7494" color="#a020f0" type="standard"/><Q name="label"><V text="P792 DFTL_1548FR2R"/></Q></Feature><Feature recentID="34" name="tRNA-dihydrouridine synthase A" directionality="1" translationMW="22327.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7592" maxFusedRunOn="7592"><Segment range="6906-7488" color="#993366" type="standard" translated="1"/><Q name="label"><V text="tRNA-dihydrouridine synthase A"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="33" name="FTL_1549" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6906-7488" color="#a6acb3" type="standard"/><Q name="label"><V text="FTL_1549"/></Q></Feature><Feature recentID="32" name="hypothetical protein" directionality="1" translationMW="228.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6864-6872" color="#993366" type="standard" translated="1"/><Q name="label"><V text="hypothetical protein"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="31" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6864-7488" color="#a6acb3" type="standard"/><Q name="label"><V text="DFTL_1548_FR2"/></Q></Feature><Feature recentID="30" name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6855-6859" color="#a6acb3" type="standard"/><Q name="label"><V text="FTL_0402"/></Q><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q></Feature><Feature recentID="29" name="P791 DFTL_1548FR2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6855-6893" color="#a020f0" type="standard"/><Q name="label"><V text="P791 DFTL_1548FR2F"/></Q></Feature><Feature recentID="28" name="FTL_1545 hypothetical protein" directionality="1" translationMW="387.52" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6872" maxFusedRunOn="6872"><Segment range="6846-6854" color="#993366" type="standard" translated="1"/><Q name="label"><V text="FTL_1545 hypothetical protein"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="27" name="P790 DFTL_1548FR1R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6826-6862" color="#a020f0" type="standard"/><Q name="label"><V text="P790 DFTL_1548FR1R"/></Q></Feature><Feature recentID="26" name="P793 DFTL_1548_int1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6516-6542" color="#a020f0" type="standard"/><Q name="label"><V text="P793 DFTL_1548_int1"/></Q></Feature><Feature recentID="25" name="DNA gyrase subunit B" directionality="2" translationMW="20114.97" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="5858" maxFusedRunOn="5858"><Segment range="6108-6664" color="#993366" type="standard" translated="1"/><Q name="label"><V text="DNA gyrase subunit B"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="24" name="FTL_1547" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6108-6664" color="#a6acb3" type="standard"/><Q name="label"><V text="FTL_1547"/></Q></Feature><Feature recentID="23" name="DFTL_1548_FR1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6854" color="#a6acb3" type="standard"/><Q name="label"><V text="DFTL_1548_FR1"/></Q></Feature><Feature recentID="22" name="P789 DFTL_1548FR1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6102-6135" color="#a020f0" type="standard"/><Q name="label"><V text="P789 DFTL_1548FR1F"/></Q></Feature><Feature recentID="21" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="label"><V text="M13 fwd"/></Q><Q name="note"><V text="common sequencing primer, one of multiple similar variants"/></Q></Feature><Feature recentID="20" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="19" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="18" name="rrnB T1 terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="label"><V text="rrnB T1 terminator"/></Q><Q name="note"><V text="transcription terminator T1 from the E. coli rrnB&#10;gene"/></Q><Q name="gene"><V text="Escherichia coli rrnB"/></Q></Feature><Feature recentID="17" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="label"><V text="rrnB T2 terminator"/></Q><Q name="note"><V text="transcription terminator T2 from the E. coli rrnB&#10;gene"/></Q></Feature><Feature recentID="16" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="15" name="incP origin of transfer" type="oriT" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="14" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="13" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="label"><V text="sacB promoter"/></Q><Q name="note"><V text="sacB promoter and control region"/></Q><Q name="gene"><V text="sacR"/></Q></Feature><Feature recentID="12" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="label"><V text="P030 pEX18KanSeq6F"/></Q><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="11" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="label"><V text="P029 pEX18KanSeq5F"/></Q><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="10" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4125" color="#993366" type="standard" translated="1"/><Q name="label"><V text="SacB"/></Q><Q name="note"><V text="negative selection marker"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="secreted levansucrase that renders bacterial&#10;growth sensitive to sucrose"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="Bacillus subtilis sacB"/></Q></Feature><Feature recentID="9" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="label"><V text="AmpR promoter"/></Q><Q name="gene"><V text="bla"/></Q></Feature><Feature recentID="8" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="label"><V text="P027 pEX18KanSeq3F"/></Q><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="7" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#993366" type="standard" translated="1"/><Q name="label"><V text="KanR"/></Q><Q name="note"><V text="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="aminoglycoside phosphotransferase"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aph(3')-Ia"/></Q></Feature><Feature recentID="6" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="5" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="4" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="3" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="label"><V text="P025 pEX18KanSeq1F"/></Q><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="2" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6864-7488" color="#ffffff" type="standard"/><Q name="label"><V text="Francisella tularensis subsp. holarctica"/></Q></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6108-6854" color="#ffffff" type="standard"/><Q name="label"><V text="Francisella tularensis subsp. holarctica"/></Q></Feature><Feature recentID="0" name="/dnas_title=pKL126 pEXDFTL_0402" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8217" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL126 pEXDFTL_0402"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  q<Notes>
<UUID>d5ce20b0-36f1-4e28-abad-d9b1b21c4a7d</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Description>synthetic circular DNA</Description>
<Created UTC="4:0:0">2018.6.6</Created>
<LastModified UTC="12:27:52">2019.1.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  In       .        ..	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
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x^8-320Gx1ɳmOp(mSW gϏI1_=GlPm03.BLTڰ<os3'lYf ϭ7Pltj2i3>L}ml7̧2	=3G<Yo}L,2'0`cL2(:Moˎ~cDc)s[MpMj߳|v[L,0ҵDkt!l6_}j<T3eExBKs^7n>3Tă][8j"K
\G-V<fnx7|0UVpǋ_?(ta\)sUe˃ONV!1%upXBܠ%6ҡ qIAFONW3kC׊@0>9=81PPƔ%@-(q[`+T<jUVj*j]VfKYZYkjjifff^]VU!ZUFVVZF]:mzM*ڭzmZVyێYJLy{vW)@[HuPEj*HfϴE}&Y%RFy+DiY(UdMdz
厺xN9ciP}}VNԯi&BJ$]ـX
@N9~9NfInpKj=)EضN*Vo;uw]O6TSa!9{v;x=8X[\£&ξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	      '  +  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  :<?xml version="1.0"?><Features nextValidID="44"><Feature recentID="43" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11934" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="42" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7567-7591" color="#a6acb3" type="standard"/><Q name="note"><V text="MOOL_pBKS_FS6"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="41" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8211-8235" color="#a6acb3" type="standard"/><Q name="note"><V text="MOOL_pBSK_FS5"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="40" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9088-9117" color="#a6acb3" type="standard"/><Q name="note"><V text="MOOL_pBSK_FS4"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="39" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9886-9909" color="#a6acb3" type="standard"/><Q name="note"><V text="MOOL_pBSK_FS3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="38" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10219-10226" color="#a6acb3" type="standard"/><Q name="note"><V text="JCOL782 (3' end)"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="37" name="lacZ" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7122-10193" color="#993366" type="standard"/><Q name="label"><V text="lacZ"/></Q></Feature><Feature recentID="36" name="glyA" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="10455" maxFusedRunOn="10455"><Segment range="10513-11222" color="#993366" type="standard" translated="1"/><Q name="note"><V text="catalyzes the reaction of glycine with 5,10-methylenetetrahydrofolate to form L-serine and tetrahydrofolate"/><V text="/dnas_title=serine hydroxymethyltransferase"/></Q><Q name="locus_tag"><V text="FTL_0703"/></Q><Q name="db_xref"><V text="89256092" predef="GI"/><V text="3951072" predef="GeneID"/></Q><Q name="EC_number"><V text="2.1.2.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="serine hydroxymethyltransferase"/></Q><Q name="protein_id"><V text="YP_513454.1"/></Q><Q name="inference"><V bool="1" text="2004" predef="profile"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="glyA"/></Q></Feature><Feature recentID="35" name="glyA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="10513-11222" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0703"/></Q><Q name="db_xref"><V text="3951072" predef="GeneID"/></Q><Q name="gene"><V text="glyA"/></Q></Feature><Feature recentID="34" name="FTL_0702" directionality="2" translateFirstCodonAsMet="1" translationMW="274.33" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="10227-10235" color="#993366" type="standard" translated="1"/><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="locus_tag"><V text="FTL_0702"/></Q><Q name="db_xref"><V text="89256091" predef="GI"/><V text="3951071" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="protein_id"><V text="YP_513453.1"/></Q><Q name="inference"><V predef="ab initio prediction"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="33" name="FTL_0702" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="10227-10235" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0702"/></Q><Q name="db_xref"><V text="3951071" predef="GeneID"/></Q></Feature><Feature recentID="32" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11196-11228" color="#a6acb3" type="standard"/><Q name="note"><V text="RPp6"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="31" name="Potential -35 element" directionality="2" type="-35_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10314-10319" color="#a6acb3" type="standard"/><Q name="note"><V text="Potential -35 element"/></Q></Feature><Feature recentID="30" name="Potential -10 element" directionality="2" type="-10_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10292-10297" color="#a6acb3" type="standard"/><Q name="note"><V text="Potential -10 element"/></Q></Feature><Feature recentID="29" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10285-10285" color="#ffffff" type="standard"/><Q name="note"><V text="Potential TSS"/></Q></Feature><Feature recentID="28" name="150 bp max AT content (93.3%)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10254-10403" color="#a6acb3" type="standard"/><Q name="note"><V text="150 bp max AT content (93.3%)"/></Q></Feature><Feature recentID="27" name="PRP12" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10385-10414" color="#a020f0" type="standard"/><Q name="note"><V text="PRP12"/></Q></Feature><Feature recentID="26" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10219-10253" color="#a6acb3" type="standard"/><Q name="note"><V text="RPp4"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="25" name="FTL_0702" directionality="2" translateFirstCodonAsMet="1" translationMW="289.32" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7096" maxFusedRunOn="7096"><Segment range="7101-7110" color="#993366" type="standard" translated="1"/><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="locus_tag"><V text="FTL_0702"/></Q><Q name="db_xref"><V text="89256091" predef="GI"/><V text="3951071" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="protein_id"><V text="YP_513453.1"/></Q><Q name="inference"><V predef="ab initio prediction"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="24" name="FTL_0702" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7101-7110" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0702"/></Q><Q name="db_xref"><V text="3951071" predef="GeneID"/></Q></Feature><Feature recentID="23" name="FTL_0701" directionality="1" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7069" maxFusedRunOn="7069"><Segment range="6113-7036" color="#993366" type="standard" translated="1"/><Q name="note"><V text="/dnas_title=FAD binding family protein"/></Q><Q name="locus_tag"><V text="FTL_0701"/></Q><Q name="db_xref"><V text="89256090" predef="GI"/><V text="3951070" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="FAD binding family protein"/></Q><Q name="protein_id"><V text="YP_513452.1"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="22" name="FTL_0701" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-7036" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0701"/></Q><Q name="db_xref"><V text="3951070" predef="GeneID"/></Q></Feature><Feature recentID="21" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7082-7118" color="#a6acb3" type="standard"/><Q name="note"><V text="RPp7"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="20" name="PRP11" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6946-6976" color="#a020f0" type="standard"/><Q name="note"><V text="PRP11"/></Q></Feature><Feature recentID="19" name="P328 SP_D0702-F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6732" color="#a020f0" type="standard"/><Q name="note"><V text="P328 SP_D0702-F"/></Q></Feature><Feature recentID="18" name="P329 SP_D0702-R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6983-7010" color="#a020f0" type="standard"/><Q name="note"><V text="P329 SP_D0702-R"/></Q></Feature><Feature recentID="17" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6107-6139" color="#a6acb3" type="standard"/><Q name="note"><V text="RPp5"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="15" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="14" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11640-288" color="#ffff00" type="standard"/><Q name="note"><V text="ColE origin"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="13" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="12" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="11" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="10" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="9" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="8" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="7" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2707-4125" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="6" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11265-11284" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="5" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11243-11268" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="4" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="3" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="2" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="1" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="0" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>a35392f5-de4e-4785-8d68-c6bae0265e8e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:24:43">2019.1.21</Created>
<LastModified UTC="12:27:52">2019.1.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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j*]x޺ʣv6NRnܬ(Dmu1A2#<f5~~t,YE)}X*41/i̐Ob&nw:(Ov޻669/[T<Ϣr.li3F}YQuYS6m	ch0vx]dX[	y_kL{\GՉ}xlAm f?_)[1@A"R5>Me3昳rކ<h?yXK,ofsP<bʜ9&=EE-M{s&KB.]?ODq$eLS]e5ӁWiIMRSYp
RyQ֠MX82;{-19˲ӊ^5}jC˜Ak	{TT$l.6/;7ϬH R ۊl;8lv`E#l0%ɂt9B/7N4~6:5h,9&~:    u o  c[g    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  I<?xml version="1.0"?><Features nextValidID="48"><Feature recentID="0" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6908-6924" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lacZ" directionality="1" translationMW="116456.20" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6888-9962" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MAMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK*"/></Q></Feature><Feature recentID="2" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="label"><V text="P025 pEX18KanSeq1F"/></Q><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="3" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="4" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="5" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="6" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aph(3')-Ia"/></Q><Q name="label"><V text="KanR"/></Q><Q name="note"><V text="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes"/></Q><Q name="product"><V text="aminoglycoside phosphotransferase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature recentID="7" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="label"><V text="P027 pEX18KanSeq3F"/></Q><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="8" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="bla"/></Q><Q name="label"><V text="AmpR promoter"/></Q></Feature><Feature recentID="9" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="Bacillus subtilis sacB"/></Q><Q name="label"><V text="SacB"/></Q><Q name="note"><V text="negative selection marker"/></Q><Q name="product"><V text="secreted levansucrase that renders bacterial&#10;growth sensitive to sucrose"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISHITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK*"/></Q></Feature><Feature recentID="10" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="label"><V text="P029 pEX18KanSeq5F"/></Q><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="11" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="label"><V text="P030 pEX18KanSeq6F"/></Q><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="12" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="sacR"/></Q><Q name="label"><V text="sacB promoter"/></Q><Q name="note"><V text="sacB promoter and control region"/></Q></Feature><Feature recentID="13" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="14" name="incP origin of transfer" type="oriT" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="15" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="16" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="label"><V text="rrnB T2 terminator"/></Q><Q name="note"><V text="transcription terminator T2 from the E. coli rrnB&#10;gene"/></Q></Feature><Feature recentID="17" name="rrnB T1 terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="Escherichia coli rrnB"/></Q><Q name="label"><V text="rrnB T1 terminator"/></Q><Q name="note"><V text="transcription terminator T1 from the E. coli rrnB&#10;gene"/></Q></Feature><Feature recentID="18" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="19" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="20" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="label"><V text="M13 fwd"/></Q><Q name="note"><V text="common sequencing primer, one of multiple similar variants"/></Q></Feature><Feature recentID="21" name="P789 DFTL_1548FR1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6102-6135" color="#a020f0" type="standard"/><Q name="label"><V text="P789 DFTL_1548FR1F"/></Q></Feature><Feature recentID="22" name="DFTL_1548_FR1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6854" color="#a6acb3" type="standard"/><Q name="label"><V text="DFTL_1548_FR1"/></Q></Feature><Feature recentID="23" name="FTL_1547" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6108-6664" color="#a6acb3" type="standard"/><Q name="label"><V text="FTL_1547"/></Q></Feature><Feature recentID="24" name="DNA gyrase subunit B" directionality="2" translationMW="20114.97" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="5858" maxFusedRunOn="5858"><Segment range="6108-6664" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="label"><V text="DNA gyrase subunit B"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSENKAYDSSSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVVDNAIDEALAGYCDDIKVIINKDGSVSVSDNGRGIPTDIHKEEGRSAAEVILTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEQLKLTIYRNGIEHYQEYVHGVPQFPLKEVGSTDKTGTLITFKPSKETFSFVDFDY"/></Q></Feature><Feature recentID="25" name="P793 DFTL_1548_int1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6516-6542" color="#a020f0" type="standard"/><Q name="label"><V text="P793 DFTL_1548_int1"/></Q></Feature><Feature recentID="26" name="P790 DFTL_1548FR1R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6826-6856" color="#a020f0" type="standard"/><Q name="label"><V text="P790 DFTL_1548FR1R"/></Q></Feature><Feature recentID="27" name="FTL_1545 hypothetical protein" directionality="1" translationMW="387.52" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6875" maxFusedRunOn="6875"><Segment range="6846-6854" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="label"><V text="FTL_1545 hypothetical protein"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="LKK"/></Q></Feature><Feature recentID="28" name="P791 DFTL_1548FR2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9965-10001" color="#a020f0" type="standard"/><Q name="label"><V text="P791 DFTL_1548FR2F"/></Q></Feature><Feature recentID="29" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9972-10596" color="#a6acb3" type="standard"/><Q name="label"><V text="DFTL_1548_FR2"/></Q></Feature><Feature recentID="30" name="FTL_1549" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="10014-10596" color="#a6acb3" type="standard"/><Q name="label"><V text="FTL_1549"/></Q></Feature><Feature recentID="31" name="tRNA-dihydrouridine synthase A" directionality="1" translationMW="22327.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="10700" maxFusedRunOn="10700"><Segment range="10014-10596" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="label"><V text="tRNA-dihydrouridine synthase A"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MKITTRKICIAPMLDWTDRHYRYMMRLITRHTMLYTEMVTLNAILHGNKEFLLKYNPQENPVTLQLGGCVPKDFISCGKLAEELDYNEININVGCPSERVKKGNFGLSLMAEPELVADCVKAMKDNLNIPISVKCRIGYDHNDSYEELYNFVDKQVQAGIDYLCIHARKGWLSGLSPKENRTIPELKYDVVYSI"/></Q></Feature><Feature recentID="32" name="P792 DFTL_1548FR2R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10569-10602" color="#a020f0" type="standard"/><Q name="label"><V text="P792 DFTL_1548FR2R"/></Q></Feature><Feature recentID="33" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10623-10639" color="#a020f0" type="standard"/><Q name="label"><V text="M13 rev"/></Q><Q name="note"><V text="common sequencing primer, one of multiple similar variants"/></Q></Feature><Feature recentID="34" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10634-10659" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="35" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10647-10663" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="lac repressor encoded by lacI"/></Q><Q name="label"><V text="lac operator"/></Q><Q name="note"><V text="The lac repressor binds to the lac operator to&#10;inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."/></Q></Feature><Feature recentID="36" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10656-10675" color="#a020f0" type="standard"/><Q name="label"><V text="P022 pEX18SeqR1"/></Q><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="37" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10671-10701" color="#ffffff" type="standard"/><Q name="label"><V text="lac promoter"/></Q><Q name="note"><V text="promoter for the E. coli lac operon"/></Q></Feature><Feature recentID="38" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10716-10737" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="E. coli catabolite activator protein"/></Q><Q name="label"><V text="CAP binding site"/></Q><Q name="note"><V text="CAP binding activates transcription in the presence&#10;of cAMP."/></Q></Feature><Feature recentID="39" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11025-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ori"/></Q><Q name="note"><V text="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"/></Q></Feature><Feature recentID="40" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7167-7190" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="41" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7959-7988" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="42" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8841-8865" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="43" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9485-9509" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="44" name="Potential chaC TSS" directionality="1" type="prim_transcript" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6814-6814" color="#a6acb3" type="standard"/></Feature><Feature recentID="45" name="Potential extended -10" type="-10_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6798-6806" color="#a6acb3" type="standard"/></Feature><Feature recentID="46" name="Potential -35" type="-35_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6771-6776" color="#a6acb3" type="standard"/></Feature><Feature recentID="47" name="Potential RBS" type="RBS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6875-6880" color="#a6acb3" type="standard"/></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="1"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="9751-9774" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="9751-9774" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="9751-9774" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="9751-9774" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer></Primers>
  i<Notes>
<UUID>51424f5c-dee6-4f2b-bcec-ccbb1b991f73</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:28:34">2019.1.21</Created>
<LastModified UTC="19:48:47">2019.8.14</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         
Unique Cutters                                                                                               d     CHO d              E              Y? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
