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gagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaaggatggatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaagcgctgcttccctgctgttttgtggaatatctaccgactggaaacaggcaaatgcaggaaattactgaactgaggggacaggcgagagacgatgccaaagagctacaccgacgagctggccgagtgggttgaatcccgcgcggccaagaagcgccggcgtgatgaggctgcggttgcgttcctggcggtgagggcggatgtcgatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgcatatttgaatgtatttagaaaaataaacaaaagagtttgtagaaacgcaaaaaggccatccgtcaggatggccttctgcttaatttgatgcctggcagtttatggcgggcgtcctgcccgccaccctccgggccgttgcttcgcaacgttcaaatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgccgccaggcaaattctgttttatcagaccgcttctgcgttctgatttaatctgtatcaggctgaaaatcttctctcatccgccaaaacagccaagctcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgccaagcttgcatgcctgcaggtcgactctagaggatcctcataatcaaagtcaacaaatgaaaacgtttctttactcggtttaaaagtaattagtgttccggtcttatctgttgaaccaacttcttttaatggaaactgtggtactccatgaacatactcttgatagtgctcgataccgtttctataaattgttaactttaattgttctgaaagcgcatttacaactgatacaccaacaccatgtaaaccgcctgatactttataagagttatcatcaaacttaccacctgcatgcaatactgtcaaaataacctctgctgcagagcgcccttcttctttatgaatatctgtaggaatacctctaccattatctgatacagatacagagccatccttatttataataactttaatatcatcacaataaccagctaaggcttcatcgatagcattatcaacaacctcaaataccatatgatgtaagccgctaccatcatcggtatcaccaatatacattccaggtctttttctaacagcatctaaacctttaagtaccttaatactcgatgagtcataagctttattctcagacattaacaaactccaaatttatcgttatattttaaaattcaatcattaagtattataacacaaataaatcttgattattaatgttgataacaatgtgagttaataaacattgagggtttttcattttatccaaaaatgctaatattaactctaacagttttgcagataggttcattttgaaattttgaaaaaggcGGCCGCTTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACGGGGTATACATGTCTGACAATGGCAGATCCCAGCGGTCAAAACAGGCGGCAGTAAGGCGGTCGGGATAGTTTTCTTGCGGCCCTAATCCGAGCCAGTTTACCCGCTCTGCTACCTGCGCCAGCTGGCAGTTCAGGCCAATCCGCGCCGGATGCGGTGTATCGCTCGCCACTTCAACATCAACGGTAATCGCCATTTGACCACTACCATCAATCCGGTAGGTTTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCACGCGTGAGCGGTCGTAATCAGCACCGCATCAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGACCCAGGCGTTAGGGTCAATGCGGGTCGCTTCACTTACGCCAATGTCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTTCGGCGTATCGCCAAAATCACCGCCGTAAGCCGACCACGGGTTGCCGTTTTCATCATATTTAATCAGCGACTGATCCACCCAGTCCCAGACGAAGCCGCCCTGTAAACGGGGATACTGACGAAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTGATTACGATCGCGCTGCACCATTCGCGTTACGCGTTCGCTCATCGCCGGTAGCCAGCGCGGATCATCGGTCAGACGATTCATTGGCACCATGCCGTGGGTTTCAATATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCAACTCGCCGCACATCTGAACTTCAGCCTCCAGTACAGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGACGTCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCGCTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCCAGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATCAACATTAAATGTGAGCGAGTAACAACCCGTCGGATTCTCCGTGGGAACAAACGGCGGATTGACCGTAATGGGATAGGTCACGTTGGTGTAGATGGGCGCATCGTAACCGTGCATCTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGCCATGGCTGTTCCTCCTTATAAAGTTAAGgcggccgctcctt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/!]+(6>PL!4%&&)++/, T<	P8XI""#%R&&'d*+#  >#~ITh		
i)SEvc#$d%*q*R+,", S\$i/[ % r n!$&9(( e	O7MS?3 #"+s+~+++ }g	(h.m!+ +!+q+r+,,,  6  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GAGNNNNNATC,GAGNNNNNGTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                     >                                                                                                                                                      	                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
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                                                                                                                                          	                                                                       B               
                                                                   +                                                                                             !                                    H   +                                 +         
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                      &                     '               	   8   C   I      /   @   	   *   3   '   &            2   2         B      !                                                                                                                                                             >                                                                      	                                                                                                                                                                                                                  
          	            
                                                      	                         B     
                       +                                 !            H +           +   
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        &       '     	 8 C I  / @ 	 * 3 ' &    2 2   B  !  m      Q    /  
9+s !"u I	*r)*:4 s !""#$%5***+"!%/""1 yh g|(%#H !q
!^)%+OR&&4c!cc!c@*XvS 'OR[dS<;[l}0/]!;"Y"$&&&&D&))M**+B)
!!! 5T!%&*Ij%)>'&&$Zc et$ DkD%)NB)d$X*	  %yx$	D9&&&(y	R$W)(1& ^#d)r!#%	&d))Tb$&)*	*
wQ !$&&&2*{3^'$0''p) #ry	ZR$C!*|o
o[!-j),o
!,l!Q`()(*o
!s,&-j)bo#	"+r .	? +{!($ &^ sT9%'Y)+ruCwjX!A#$&U*+ArW	!!#%	&d))*+ #$% 6'=+<!o#E&d,)}|S\[!\!!q%&&)s++4,$]EO! &n!$,['#!!l"<(!"o#E&&|*$,	 	P$&o
o[!s-j),of&		*&=JS&Y*4 $QF
yKmqu2EOSDX\YdhDN   !""
##>%%M&a&e&(	(****+!\!!q%&
t"%	*,"xh6gt !"t$_)	m#''%S!i$++-j)Df"  ##$%.  
I^`"""" kOa{~p)dn1
t"%k((o

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<:Zf	 '"#c&!*r*',%lZfc&!* l6'=+	!0![ !{*e
o1#d^!x#<%i'(xb6 /!! #zi =!|&T]wI:\g $&&r')*g+t+	#		$ 	dP$- 0  	9F&o]0c}!""H$$/&x&5(#*t**),<'R	?Zo"F=C O& >~T	
vc $!		:!~p##$o'*)' - 0  	9]""H$$F&o0c}!/&x&5(#*t**),LF,~r:u s q"$%]	ci+Z ,"%| -}F""$}%& x )![!!""#$$- 0  !p)r	T
vOX9F&o];0H8c}/ !!""##$H$a$$/&x&&&5(#*t****+), >~T|	
&T]wI:\gvc  $!$&&r')*g+t+xW
Y<%%++&c2i

4j'E$"d') 6 ($hqH' } %K$  S #%&&*

i*+""r& M y-;! !}"g$B<T$%+i
O1JC)3%#  ' 		
+4.<d:k "I##$%%%7&'}([)**B~	i
<OT1eJ$%)C)+DQ %&( 5 t$H_)k*>Ps5Zk#9+X*
B M!,%g'ISE*q*X0
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  ;S<?xml version="1.0"?><Features nextValidID="44"><Feature recentID="43" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7917-288" color="#ffff00" type="standard"/><Q name="label"><V text="ori"/></Q><Q name="note"><V text="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="42" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7608-7629" color="#31849b" type="standard"/><Q name="label"><V text="CAP binding site"/></Q><Q name="note"><V text="CAP binding activates transcription in the presence&#10;of cAMP."/></Q><Q name="bound_moiety"><V text="E. coli catabolite activator protein"/></Q></Feature><Feature recentID="41" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7563-7593" color="#ffffff" type="standard"/><Q name="label"><V text="lac promoter"/></Q><Q name="note"><V text="promoter for the E. coli lac operon"/></Q></Feature><Feature recentID="40" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7548-7567" color="#a020f0" type="standard"/><Q name="label"><V text="P022 pEX18SeqR1"/></Q><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="39" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7539-7555" color="#31849b" type="standard"/><Q name="label"><V text="lac operator"/></Q><Q name="note"><V text="The lac repressor binds to the lac operator to&#10;inhibit transcription in E. coli. 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allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7489-7493" color="#a6acb3" type="standard"/><Q name="label"><V text="FTL_0404"/></Q><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="3952331" predef="GeneID"/></Q></Feature><Feature recentID="35" name="P792 DFTL_1548FR2R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7461-7494" color="#a020f0" type="standard"/><Q name="label"><V text="P792 DFTL_1548FR2R"/></Q></Feature><Feature recentID="34" name="tRNA-dihydrouridine synthase A" directionality="1" translationMW="22327.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7592" maxFusedRunOn="7592"><Segment range="6906-7488" color="#993366" type="standard" translated="1"/><Q name="label"><V text="tRNA-dihydrouridine synthase A"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V 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allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6855-6859" color="#a6acb3" type="standard"/><Q name="label"><V text="FTL_0402"/></Q><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q></Feature><Feature recentID="29" name="P791 DFTL_1548FR2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6855-6893" color="#a020f0" type="standard"/><Q name="label"><V text="P791 DFTL_1548FR2F"/></Q></Feature><Feature recentID="28" name="FTL_1545 hypothetical protein" directionality="1" translationMW="387.52" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6872" maxFusedRunOn="6872"><Segment range="6846-6854" color="#993366" type="standard" translated="1"/><Q name="label"><V text="FTL_1545 hypothetical protein"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="27" name="P790 DFTL_1548FR1R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6826-6862" color="#a020f0" type="standard"/><Q name="label"><V text="P790 DFTL_1548FR1R"/></Q></Feature><Feature recentID="26" name="P793 DFTL_1548_int1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6516-6542" color="#a020f0" type="standard"/><Q name="label"><V text="P793 DFTL_1548_int1"/></Q></Feature><Feature recentID="25" name="DNA gyrase subunit B" directionality="2" translationMW="20114.97" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="5858" maxFusedRunOn="5858"><Segment range="6108-6664" color="#993366" type="standard" translated="1"/><Q name="label"><V text="DNA gyrase subunit B"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="24" name="FTL_1547" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6108-6664" color="#a6acb3" type="standard"/><Q name="label"><V text="FTL_1547"/></Q></Feature><Feature recentID="23" name="DFTL_1548_FR1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6854" color="#a6acb3" type="standard"/><Q name="label"><V text="DFTL_1548_FR1"/></Q></Feature><Feature recentID="22" name="P789 DFTL_1548FR1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6102-6135" color="#a020f0" type="standard"/><Q name="label"><V text="P789 DFTL_1548FR1F"/></Q></Feature><Feature recentID="21" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="label"><V text="M13 fwd"/></Q><Q name="note"><V text="common sequencing primer, one of multiple similar variants"/></Q></Feature><Feature recentID="20" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="19" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="18" name="rrnB T1 terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="label"><V text="rrnB T1 terminator"/></Q><Q name="note"><V text="transcription terminator T1 from the E. coli rrnB&#10;gene"/></Q><Q name="gene"><V text="Escherichia coli rrnB"/></Q></Feature><Feature recentID="17" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="label"><V text="rrnB T2 terminator"/></Q><Q name="note"><V text="transcription terminator T2 from the E. coli rrnB&#10;gene"/></Q></Feature><Feature recentID="16" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="15" name="incP origin of transfer" type="oriT" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="14" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="13" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="label"><V text="sacB promoter"/></Q><Q name="note"><V text="sacB promoter and control region"/></Q><Q name="gene"><V text="sacR"/></Q></Feature><Feature recentID="12" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="label"><V text="P030 pEX18KanSeq6F"/></Q><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="11" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="label"><V text="P029 pEX18KanSeq5F"/></Q><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="10" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4125" color="#993366" type="standard" translated="1"/><Q name="label"><V text="SacB"/></Q><Q name="note"><V text="negative selection marker"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="secreted levansucrase that renders bacterial&#10;growth sensitive to sucrose"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="Bacillus subtilis sacB"/></Q></Feature><Feature recentID="9" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="label"><V text="AmpR promoter"/></Q><Q name="gene"><V text="bla"/></Q></Feature><Feature recentID="8" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="label"><V text="P027 pEX18KanSeq3F"/></Q><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="7" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#993366" type="standard" translated="1"/><Q name="label"><V text="KanR"/></Q><Q name="note"><V text="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="aminoglycoside phosphotransferase"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aph(3')-Ia"/></Q></Feature><Feature recentID="6" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="5" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="4" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="3" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="label"><V text="P025 pEX18KanSeq1F"/></Q><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="2" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6864-7488" color="#ffffff" type="standard"/><Q name="label"><V text="Francisella tularensis subsp. holarctica"/></Q></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6108-6854" color="#ffffff" type="standard"/><Q name="label"><V text="Francisella tularensis subsp. holarctica"/></Q></Feature><Feature recentID="0" name="/dnas_title=pKL126 pEXDFTL_0402" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8217" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL126 pEXDFTL_0402"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  p<Notes>
<UUID>37130c76-ad1c-496c-9929-52cef5beaa70</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Description>synthetic circular DNA</Description>
<Created UTC="4:0:0">2018.6.6</Created>
<LastModified UTC="17:5:55">2019.1.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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\G-V<fnx7|0UVpǋ_?(ta\)sUe˃ONV!1%upXBܠ%6ҡ qIAFONW3kC׊@0>9=81PPƔ%@-(q[`+T<jUVj*j]VfKYZYkjjifff^]VU!ZUFVVZF]:mzM*ڭzmZVyێYJLy{vW)@[HuPEj*HfϴE}&Y%RFy+DiY(UdMdz
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ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	      '  +  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  T<?xml version="1.0"?><Features nextValidID="55"><Feature recentID="54" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11256-11272" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="53" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11232-11248" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="52" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10152-10168" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="51" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="50" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11325-11346" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="49" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="48" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="11280-11286" color="#ffffff" type="standard"/><Segment range="11287-11304" color="#ffffff" type="standard"/><Segment name="-35" range="11305-11310" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="47" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="46" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="45" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="44" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="43" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11634-288" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="42" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="lacZ" directionality="2" translationMW="116456.20" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="7114-10188" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="38" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="37" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="36" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="35" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="34" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11243-11268" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="33" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11265-11284" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="32" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="31" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="30" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="29" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="28" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="27" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="26" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="25" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6107-6139" color="#a6acb3" type="standard"/><Q name="note"><V text="RPp5"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="24" name="P329 SP_D0702-R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6983-7010" color="#a020f0" type="standard"/><Q name="note"><V text="P329 SP_D0702-R"/></Q></Feature><Feature recentID="23" name="P328 SP_D0702-F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6732" color="#a020f0" type="standard"/><Q name="note"><V text="P328 SP_D0702-F"/></Q></Feature><Feature recentID="22" name="PRP11" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6946-6976" color="#a020f0" type="standard"/><Q name="note"><V text="PRP11"/></Q></Feature><Feature recentID="21" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7082-7118" color="#a6acb3" type="standard"/><Q name="note"><V text="RPp7"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="20" name="FTL_0701" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-7036" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0701"/></Q><Q name="db_xref"><V text="3951070" predef="GeneID"/></Q></Feature><Feature recentID="19" name="FTL_0701" directionality="1" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7069" maxFusedRunOn="7069"><Segment range="6113-7036" color="#993366" type="standard" translated="1"/><Q name="note"><V text="/dnas_title=FAD binding family protein"/></Q><Q name="locus_tag"><V text="FTL_0701"/></Q><Q name="db_xref"><V text="89256090" predef="GI"/><V text="3951070" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="FAD binding family protein"/></Q><Q name="protein_id"><V text="YP_513452.1"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="18" name="FTL_0702" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7101-7110" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0702"/></Q><Q name="db_xref"><V text="3951071" predef="GeneID"/></Q></Feature><Feature recentID="17" name="FTL_0702" directionality="2" translateFirstCodonAsMet="1" translationMW="289.32" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7096" maxFusedRunOn="7096"><Segment range="7101-7110" color="#993366" type="standard" translated="1"/><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="locus_tag"><V text="FTL_0702"/></Q><Q name="db_xref"><V text="89256091" predef="GI"/><V text="3951071" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="protein_id"><V text="YP_513453.1"/></Q><Q name="inference"><V predef="ab initio prediction"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="16" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10219-10253" color="#a6acb3" type="standard"/><Q name="note"><V text="RPp4"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="15" name="PRP12" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10385-10414" color="#a020f0" type="standard"/><Q name="note"><V text="PRP12"/></Q></Feature><Feature recentID="14" name="150 bp max AT content (93.3%)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10254-10403" color="#a6acb3" type="standard"/><Q name="note"><V text="150 bp max AT content (93.3%)"/></Q></Feature><Feature recentID="13" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10285-10285" color="#ffffff" type="standard"/><Q name="note"><V text="Potential TSS"/></Q></Feature><Feature recentID="12" name="Potential -10 element" directionality="2" type="-10_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10292-10297" color="#a6acb3" type="standard"/><Q name="note"><V text="Potential -10 element"/></Q></Feature><Feature recentID="11" name="Potential -35 element" directionality="2" type="-35_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10314-10319" color="#a6acb3" type="standard"/><Q name="note"><V text="Potential -35 element"/></Q></Feature><Feature recentID="10" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11196-11228" color="#a6acb3" type="standard"/><Q name="note"><V text="RPp6"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="9" name="FTL_0702" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="10227-10235" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0702"/></Q><Q name="db_xref"><V text="3951071" predef="GeneID"/></Q></Feature><Feature recentID="8" name="FTL_0702" directionality="2" translateFirstCodonAsMet="1" translationMW="274.33" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="10227-10235" color="#993366" type="standard" translated="1"/><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="locus_tag"><V text="FTL_0702"/></Q><Q name="db_xref"><V text="89256091" predef="GI"/><V text="3951071" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="protein_id"><V text="YP_513453.1"/></Q><Q name="inference"><V predef="ab initio prediction"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="7" name="glyA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="10513-11222" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0703"/></Q><Q name="db_xref"><V text="3951072" predef="GeneID"/></Q><Q name="gene"><V text="glyA"/></Q></Feature><Feature recentID="6" name="glyA" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="10455" maxFusedRunOn="10455"><Segment range="10513-11222" color="#993366" type="standard" translated="1"/><Q name="note"><V text="catalyzes the reaction of glycine with 5,10-methylenetetrahydrofolate to form L-serine and tetrahydrofolate"/><V text="/dnas_title=serine hydroxymethyltransferase"/></Q><Q name="locus_tag"><V text="FTL_0703"/></Q><Q name="db_xref"><V text="89256092" predef="GI"/><V text="3951072" predef="GeneID"/></Q><Q name="EC_number"><V text="2.1.2.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="serine hydroxymethyltransferase"/></Q><Q name="protein_id"><V text="YP_513454.1"/></Q><Q name="inference"><V bool="1" text="2004" predef="profile"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="glyA"/></Q></Feature><Feature recentID="5" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9886-9909" color="#a6acb3" type="standard"/><Q name="note"><V text="MOOL_pBSK_FS3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="4" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9088-9117" color="#a6acb3" type="standard"/><Q name="note"><V text="MOOL_pBSK_FS4"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="3" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8211-8235" color="#a6acb3" type="standard"/><Q name="note"><V text="MOOL_pBSK_FS5"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="2" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7567-7591" color="#a6acb3" type="standard"/><Q name="note"><V text="MOOL_pBKS_FS6"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="1" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11934" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="0" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10219-10226" color="#a6acb3" type="standard"/><Q name="note"><V text="JCOL782 (3' end)"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  B<Notes>
<UUID>fef7c006-5cfd-4f3f-9822-baab5e3c19da</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:24:43">2019.1.21</Created>
<LastModified UTC="17:5:55">2019.1.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  P@<?xml version="1.0"?><HistoryTree><Node name="Cloned.dna" type="DNA" seqLen="11325" ID="2" circular="1" operation="insertFragment"><RegeneratedSite name="NotI" pos="6856" siteCount="2"/><RegeneratedSite name="NotI" pos="9964" siteCount="2"/><HistoryColors><StrandColors type="Product"><TopStrand><ColorRange range="6856..9963" colors="red"/></TopStrand><BottomStrand><ColorRange range="6860..9967" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="insertAt" name1="NotI" val1="6856" siteCount1="1"/><InputSummary manipulation="insert" name1="NotI" name2="NotI" val1="7107" val2="10215" siteCount1="2" siteCount2="2"/><Node name="pKL133 pEXDFTL_1548.gb" type="DNA" seqLen="8217" ID="0" circular="1" resurrectable="1" operation="invalid"><Features><Feature name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2704-4125" color="#993366" type="standard"/><Q name="label"><V text="SacB"/></Q><Q name="note"><V text="negative selection marker"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="secreted levansucrase that renders bacterial&#10;growth sensitive to sucrose"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="Bacillus subtilis sacB"/></Q><Q name="translation"><V text="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISHITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK*"/></Q></Feature><Feature name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0"><Segment range="1104-1919" color="#993366" type="standard"/><Q name="label"><V text="KanR"/></Q><Q name="note"><V text="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="aminoglycoside phosphotransferase"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aph(3')-Ia"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature name="DFTL_1548_FR1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="6108-6854" color="#a6acb3" type="standard"/><Q name="label"><V text="DFTL_1548_FR1"/></Q></Feature><Feature name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="6864-7488" color="#a6acb3" type="standard"/><Q name="label"><V text="DFTL_1548_FR2"/></Q></Feature><Feature name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="7917-288" color="#ffff00" type="standard"/><Q name="label"><V text="ori"/></Q><Q name="note"><V text="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="label"><V text="sacB promoter"/></Q><Q name="note"><V text="sacB promoter and control region"/></Q><Q name="gene"><V text="sacR"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature name="incP origin of transfer" type="oriT" allowSegmentOverlaps="0"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="label"><V text="AmpR promoter"/></Q><Q name="gene"><V text="bla"/></Q></Feature><Feature name="rrnB T1 terminator" type="terminator" allowSegmentOverlaps="0"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="label"><V text="rrnB T1 terminator"/></Q><Q name="note"><V text="transcription terminator T1 from the E. coli rrnB&#10;gene"/></Q><Q name="gene"><V text="Escherichia coli rrnB"/></Q></Feature><Feature name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="7563-7593" color="#ffffff" type="standard"/><Q name="label"><V text="lac promoter"/></Q><Q name="note"><V text="promoter for the E. coli lac operon"/></Q></Feature><Feature name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="label"><V text="rrnB T2 terminator"/></Q><Q name="note"><V text="transcription terminator T2 from the E. coli rrnB&#10;gene"/></Q></Feature><Feature name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="7526-7551" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0"><Segment range="7608-7629" color="#31849b" type="standard"/><Q name="label"><V text="CAP binding site"/></Q><Q name="note"><V text="CAP binding activates transcription in the presence&#10;of cAMP."/></Q><Q name="bound_moiety"><V text="E. coli catabolite activator protein"/></Q></Feature><Feature name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="6855-6859" color="#a6acb3" type="standard"/><Q name="label"><V text="FTL_0402"/></Q><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q></Feature><Feature name="FTL_0404" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="7489-7493" color="#a6acb3" type="standard"/><Q name="label"><V text="FTL_0404"/></Q><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="3952331" predef="GeneID"/></Q></Feature></Features></Node><Node name="pKL61 pEXPFTL_0702-lacZ.dna" type="DNA" seqLen="11934" ID="1" circular="1" resurrectable="1" operation="invalid"><Features><Feature name="lacZ" directionality="2" translationMW="116456.20" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="7114-10188" color="#993366" type="standard"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="translation"><V text="MAMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK*"/></Q></Feature><Feature name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038"><Segment range="2704-4038" color="#993366" type="standard"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="translation"><V text="MNIKKFAKQATVLTFTTALLAGGATQAFA,KETNQKPYKETYGISHITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK*"/></Q></Feature><Feature name="FTL_0701" directionality="1" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0"><Segment range="6113-7036" color="#993366" type="standard"/><Q name="note"><V text="/dnas_title=FAD binding family protein"/></Q><Q name="locus_tag"><V text="FTL_0701"/></Q><Q name="db_xref"><V text="89256090" predef="GI"/><V text="3951070" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="FAD binding family protein"/></Q><Q name="protein_id"><V text="YP_513452.1"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="translation"><V text="PDIKNTQTPKSLWTDKWRNNRRF**GL*KFDKRVSFILLFKVE**TLGRTICISS***DNDSYSDSRFS*KRSFGYLAAI*RVAKEPV*HTLQA*IYRYSTSTNLEL*FLA*KLP*YGY*KYSSRCSRWRILVGFKYW*SISLLVHLSILVLTGEVI*F*KYRQNCGYFL*SFTICASINSD**RLSRSF*TSYTIN*TDIYASRCLRCWCFSNYEL*Y*QATVW*AKDDSRD*TESR*YLQSYEHDYSFSS*CWDICK*S*LLPKKLAASILG**LL*IT*D*KQI*SKWSILLSSLCW**ILAIGW"/></Q></Feature><Feature name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0"><Segment range="1104-1919" color="#ccffcc" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature name="glyA" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="10513-11222" color="#993366" type="standard"/><Q name="note"><V text="catalyzes the reaction of glycine with 5,10-methylenetetrahydrofolate to form L-serine and tetrahydrofolate"/><V text="/dnas_title=serine hydroxymethyltransferase"/></Q><Q name="locus_tag"><V text="FTL_0703"/></Q><Q name="db_xref"><V text="89256092" predef="GI"/><V text="3951072" predef="GeneID"/></Q><Q name="EC_number"><V text="2.1.2.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="serine hydroxymethyltransferase"/></Q><Q name="protein_id"><V text="YP_513454.1"/></Q><Q name="inference"><V bool="1" text="2004" predef="profile"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="glyA"/></Q><Q name="translation"><V text="NS*LAKVQRNSRLRRCRTYGRYSSCSRSSSSWSIP*PIPICLCCNYNYSQDIKGT*RWINTL***SGTCKKIPISYFPWYSRWSFNACNSS*SSCI*RSIRTKFCRLSKTSIKKC*GYGKSFKTTWY*YNLWWN**SSTFT*YYKYWFLW*RS*GGSR*SKYHC**KLYPK*SPFSFCH*RIKDWKPCYNDERFQRERM*VSR*LISRCSI*LW**KS*K*NCC*SFRSL**APCL"/></Q></Feature><Feature name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="11634-288" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature name="150 bp max AT content (93.3%)" type="misc_feature" allowSegmentOverlaps="0"><Segment range="10254-10403" color="#a6acb3" type="standard"/><Q name="note"><V text="150 bp max AT content (93.3%)"/></Q></Feature><Feature name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0"><Segment range="10219-10253" color="#a6acb3" type="standard"/><Q name="note"><V text="RPp4"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment name="-10" range="11280-11286" color="#ffffff" type="standard"/><Segment range="11287-11304" color="#ffffff" type="standard"/><Segment name="-35" range="11305-11310" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="11243-11268" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="11325-11346" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature name="FTL_0702" directionality="2" translateFirstCodonAsMet="1" translationMW="289.32" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="7101-7110" color="#993366" type="standard"/><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="locus_tag"><V text="FTL_0702"/></Q><Q name="db_xref"><V text="89256091" predef="GI"/><V text="3951071" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="protein_id"><V text="YP_513453.1"/></Q><Q name="inference"><V predef="ab initio prediction"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="translation"><V text="AAE"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="7107..10214" colors="red"/><ColorRange range="0..7106" colors="white"/><ColorRange range="10215..11933" colors="white"/></TopStrand><BottomStrand><ColorRange range="7111..10218" colors="red"/><ColorRange range="0..7110" colors="white"/><ColorRange range="10219..11933" colors="white"/></BottomStrand></StrandColors></HistoryColors></Node></Node></HistoryTree>
  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  T_<?xml version="1.0"?><Features nextValidID="53"><Feature recentID="52" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="label"><V text="P025 pEX18KanSeq1F"/></Q><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="51" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="50" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="49" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="48" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#993366" type="standard" translated="1"/><Q name="label"><V text="KanR"/></Q><Q name="note"><V text="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="aminoglycoside phosphotransferase"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aph(3')-Ia"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature recentID="47" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="label"><V text="P027 pEX18KanSeq3F"/></Q><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="46" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="label"><V text="AmpR promoter"/></Q><Q name="gene"><V text="bla"/></Q></Feature><Feature recentID="45" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4125" color="#993366" type="standard" translated="1"/><Q name="label"><V text="SacB"/></Q><Q name="note"><V text="negative selection marker"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="secreted levansucrase that renders bacterial&#10;growth sensitive to sucrose"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="Bacillus subtilis sacB"/></Q><Q name="translation"><V text="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISHITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK*"/></Q></Feature><Feature recentID="44" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="label"><V text="P029 pEX18KanSeq5F"/></Q><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="43" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="label"><V text="P030 pEX18KanSeq6F"/></Q><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="42" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="label"><V text="sacB promoter"/></Q><Q name="note"><V text="sacB promoter and control region"/></Q><Q name="gene"><V text="sacR"/></Q></Feature><Feature recentID="41" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="40" name="incP origin of transfer" type="oriT" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="39" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="38" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="label"><V text="rrnB T2 terminator"/></Q><Q name="note"><V text="transcription terminator T2 from the E. coli rrnB&#10;gene"/></Q></Feature><Feature recentID="37" name="rrnB T1 terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="label"><V text="rrnB T1 terminator"/></Q><Q name="note"><V text="transcription terminator T1 from the E. coli rrnB&#10;gene"/></Q><Q name="gene"><V text="Escherichia coli rrnB"/></Q></Feature><Feature recentID="36" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="35" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="34" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="label"><V text="M13 fwd"/></Q><Q name="note"><V text="common sequencing primer, one of multiple similar variants"/></Q></Feature><Feature recentID="33" name="P789 DFTL_1548FR1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6102-6135" color="#a020f0" type="standard"/><Q name="label"><V text="P789 DFTL_1548FR1F"/></Q></Feature><Feature recentID="32" name="DFTL_1548_FR1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6854" color="#a6acb3" type="standard"/><Q name="label"><V text="DFTL_1548_FR1"/></Q></Feature><Feature recentID="31" name="FTL_1547" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6108-6664" color="#a6acb3" type="standard"/><Q name="label"><V text="FTL_1547"/></Q></Feature><Feature recentID="30" name="DNA gyrase subunit B" directionality="2" translationMW="20114.97" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="5858" maxFusedRunOn="5858"><Segment range="6108-6664" color="#993366" type="standard" translated="1"/><Q name="label"><V text="DNA gyrase subunit B"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="translation"><V text="MSENKAYDSSSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVVDNAIDEALAGYCDDIKVIINKDGSVSVSDNGRGIPTDIHKEEGRSAAEVILTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEQLKLTIYRNGIEHYQEYVHGVPQFPLKEVGSTDKTGTLITFKPSKETFSFVDFDY"/></Q></Feature><Feature recentID="29" name="P793 DFTL_1548_int1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6516-6542" color="#a020f0" type="standard"/><Q name="label"><V text="P793 DFTL_1548_int1"/></Q></Feature><Feature recentID="28" name="P790 DFTL_1548FR1R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9965-9970" color="#a020f0" type="standard"/><Q name="label"><V text="P790 DFTL_1548FR1R"/></Q></Feature><Feature recentID="27" name="P790 DFTL_1548FR1R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6826-6856" color="#a020f0" type="standard"/><Q name="label"><V text="P790 DFTL_1548FR1R"/></Q></Feature><Feature recentID="26" name="FTL_1545 hypothetical protein" directionality="1" translationMW="387.52" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6872" maxFusedRunOn="6872"><Segment range="6846-6854" color="#993366" type="standard" translated="1"/><Q name="label"><V text="FTL_1545 hypothetical protein"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="translation"><V text="LKK"/></Q></Feature><Feature recentID="25" name="P791 DFTL_1548FR2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9965-10001" color="#a020f0" type="standard"/><Q name="label"><V text="P791 DFTL_1548FR2F"/></Q></Feature><Feature recentID="24" name="P791 DFTL_1548FR2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6855-6856" color="#a020f0" type="standard"/><Q name="label"><V text="P791 DFTL_1548FR2F"/></Q></Feature><Feature recentID="23" name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9965-9967" color="#a6acb3" type="standard"/><Q name="label"><V text="FTL_0402"/></Q><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q></Feature><Feature recentID="22" name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6855-6856" color="#a6acb3" type="standard"/><Q name="label"><V text="FTL_0402"/></Q><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q></Feature><Feature recentID="21" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9972-10596" color="#a6acb3" type="standard"/><Q name="label"><V text="DFTL_1548_FR2"/></Q></Feature><Feature recentID="20" name="hypothetical protein" directionality="1" translationMW="228.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="9972-9980" color="#993366" type="standard" translated="1"/><Q name="label"><V text="hypothetical protein"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="translation"><V text="PL*"/></Q></Feature><Feature recentID="19" name="FTL_1549" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="10014-10596" color="#a6acb3" type="standard"/><Q name="label"><V text="FTL_1549"/></Q></Feature><Feature recentID="18" name="tRNA-dihydrouridine synthase A" directionality="1" translationMW="22327.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="10700" maxFusedRunOn="10700"><Segment range="10014-10596" color="#993366" type="standard" translated="1"/><Q name="label"><V text="tRNA-dihydrouridine synthase A"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="translation"><V text="MKITTRKICIAPMLDWTDRHYRYMMRLITRHTMLYTEMVTLNAILHGNKEFLLKYNPQENPVTLQLGGCVPKDFISCGKLAEELDYNEININVGCPSERVKKGNFGLSLMAEPELVADCVKAMKDNLNIPISVKCRIGYDHNDSYEELYNFVDKQVQAGIDYLCIHARKGWLSGLSPKENRTIPELKYDVVYSI"/></Q></Feature><Feature recentID="17" name="P792 DFTL_1548FR2R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10569-10602" color="#a020f0" type="standard"/><Q name="label"><V text="P792 DFTL_1548FR2R"/></Q></Feature><Feature recentID="16" name="FTL_0404" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10597-10601" color="#a6acb3" type="standard"/><Q name="label"><V text="FTL_0404"/></Q><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="3952331" predef="GeneID"/></Q></Feature><Feature recentID="15" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10623-10639" color="#a020f0" type="standard"/><Q name="label"><V text="M13 rev"/></Q><Q name="note"><V text="common sequencing primer, one of multiple similar variants"/></Q></Feature><Feature recentID="14" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10634-10659" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="13" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10647-10663" color="#31849b" type="standard"/><Q name="label"><V text="lac operator"/></Q><Q name="note"><V text="The lac repressor binds to the lac operator to&#10;inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."/></Q><Q name="bound_moiety"><V text="lac repressor encoded by lacI"/></Q></Feature><Feature recentID="12" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10656-10675" color="#a020f0" type="standard"/><Q name="label"><V text="P022 pEX18SeqR1"/></Q><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="11" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10671-10701" color="#ffffff" type="standard"/><Q name="label"><V text="lac promoter"/></Q><Q name="note"><V text="promoter for the E. coli lac operon"/></Q></Feature><Feature recentID="10" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10716-10737" color="#31849b" type="standard"/><Q name="label"><V text="CAP binding site"/></Q><Q name="note"><V text="CAP binding activates transcription in the presence&#10;of cAMP."/></Q><Q name="bound_moiety"><V text="E. coli catabolite activator protein"/></Q></Feature><Feature recentID="9" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11025-288" color="#ffff00" type="standard"/><Q name="label"><V text="ori"/></Q><Q name="note"><V text="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="8" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7316-7340" color="#a6acb3" type="standard"/><Q name="note"><V text="MOOL_pBKS_FS6"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="7" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7960-7984" color="#a6acb3" type="standard"/><Q name="note"><V text="MOOL_pBSK_FS5"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="6" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8837-8866" color="#a6acb3" type="standard"/><Q name="note"><V text="MOOL_pBSK_FS4"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="5" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9635-9658" color="#a6acb3" type="standard"/><Q name="note"><V text="MOOL_pBSK_FS3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="4" name="FTL_0702" directionality="2" translateFirstCodonAsMet="1" translationMW="89.11" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6833" maxFusedRunOn="6833"><Segment range="6857-6859" color="#993366" type="standard" translated="1"/><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="locus_tag"><V text="FTL_0702"/></Q><Q name="db_xref"><V text="89256091" predef="GI"/><V text="3951071" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="protein_id"><V text="YP_513453.1"/></Q><Q name="inference"><V predef="ab initio prediction"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="translation"><V text="A"/></Q></Feature><Feature recentID="3" name="FTL_0702" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6857-6859" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0702"/></Q><Q name="db_xref"><V text="3951071" predef="GeneID"/></Q></Feature><Feature recentID="2" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6857-6867" color="#a6acb3" type="standard"/><Q name="note"><V text="RPp7"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="1" name="lacZ" directionality="2" translationMW="116456.20" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6863-9937" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="translation"><V text="MAMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK*"/></Q></Feature><Feature recentID="0" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9901-9917" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  e<Notes>
<UUID>c776ea49-f454-47af-a08e-87d979a67d11</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="17:6:5">2019.1.21</Created>
<LastModified UTC="17:6:5">2019.1.21</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         Unsaved Enzyme Set                                                                                                    HO  d              E                                                                                                                                                                                           <?xml version="1.0"?><CustomEnzymeSets><CustomEnzymeSet type="2" name="Unsaved Enzyme Set" enzymeNames="PciI"/></CustomEnzymeSets>
