LOCUS       pIMAY-Z                 8815 bp    DNA     circular SYN  9-JUN-2017
DEFINITION  LMBP 10259.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10259
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 9-JUN-2017
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|-11800301|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pIMAY-Z|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     promoter        1..17
                     /vntifkey="30"
                     /label=T3\promoter
     promoter        complement(150..166)
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      complement(598..1097)
                     /vntifkey="33"
                     /label=p15A\ori
     misc_RNA        1706..2259
                     /vntifkey="53"
                     /label=secY
     CDS             2411..3034
                     /vntifkey="4"
                     /label=tetR
     CDS             3849..4547
                     /vntifkey="4"
                     /label=repAts
     CDS             complement(4893..7952)
                     /vntifkey="4"
                     /label=lacZ
     CDS             complement(7965..8615)
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     rep_origin      1361..1471
                     /vntifkey="33"
                     /label=RK2/RP4\oriT
     CDS             3371..3580
                     /vntifkey="4"
                     /label=repB
     CDS             3621..3782
                     /vntifkey="4"
                     /label=repC
     CDS             4544..4693
                     /vntifkey="4"
                     /label=repD
     promoter        2271..2407
                     /vntifkey="30"
                     /label=xyl/tetO\promoter
     rep_origin      3075..4693
                     /vntifkey="33"
                     /label=pVE6007\ori
BASE COUNT     2468 a      1955 c      2055 g      2337 t 
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct gggtaccggg ccccccctcg aggtcgacgg 
       61 tatcgataag cttgatatcg aattcctgca gcccggggga tccactagtt ctagagcggc 
      121 cgccaccgcg gtggagctcc aattcgccct atagtgagtc gtattacgac gtcccagggc 
      181 ttcccggtat caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca 
      241 ggtatttatt cggcgcaaag tgcgtcgggt gatgctgcca acttactgat ttagtgtatg 
      301 atggtgtttt tgaggtgctc cagtggcttc tgtttctatc agctgtccct cctgttcagc 
      361 tactgacggg gtggtgcgta acggcaaaag caccgccgga catcagcgct agcggagtgt 
      421 atactggctt actatgttgg cactgatgag ggtgtcagtg aagtgcttca tgtggcagga 
      481 gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata caggatatat tccgcttcct 
      541 cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac 
      601 ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc 
      661 ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc 
      721 aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg 
      781 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg 
      841 gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc 
      901 tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc 
      961 gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt 
     1021 gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt 
     1081 tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga 
     1141 accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc 
     1201 agaccaaaac gatctcaaga agatcatctt attaatcaga taaaatattt ctagatttca 
     1261 gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc ccatacgata taagttgtaa 
     1321 ttctccgccg cttgccctca tctgttacgc cggcggtagc cggccagcct cgcagagcag 
     1381 gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg 
     1441 cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt 
     1501 ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa 
     1561 aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagcgctgc ttccctgctg 
     1621 ttttgtggaa tatctaccga ctggaaacag gcaaatgcag gaaattactg aactgagggg 
     1681 acaggcgaga ggagatcttg atctaatgat tcaaaccctt gtgaacttct ttagaacaaa 
     1741 agaggttcgt aacaagattt tcttcacact agcaatgtta gtaattttta aaatagggac 
     1801 ttatatacca gctccaggag taaatcctgc agcttttgat aatccccaag gttctcaagg 
     1861 tgccactgag ttattaaata cttttggtgg cggagccttg aaacgatttt ctatttttgc 
     1921 aatgggtatt gtaccctaca tcactgcatc aatcgtaatg caattattac aaatggatat 
     1981 tgtccctaaa ttctcagaat gggcaaaaca aggtgaagta ggtagaagaa agttaaataa 
     2041 cgttactcgt tatttagcaa tttctttagc atttatccaa tctataggta tggcattcca 
     2101 atttaataat tatctcaaag gtgcgctgat tatcaatcag tcaattatga gttatttatt 
     2161 aatagcacta gttttgacag caggaactgc tttcttaata tggcttggtg atcaaatcac 
     2221 tcagttcggt gttggtaatg gtatttctat tatcatattc ccatcaagct tattttaatt 
     2281 atactctatc aatgatagag tgtcaatatt ttttttagtt tttcatgaac tcgacgcgat 
     2341 ccaaataaaa aactagtttg acaaataact ctatcaatga tagagtgtca acaaaaagga 
     2401 ggaattaatg atgtctagat tagataaaag taaagtgatt aacagcgcat tagagctgct 
     2461 taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga 
     2521 gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat 
     2581 tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga 
     2641 ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc 
     2701 aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt 
     2761 agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt 
     2821 ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga 
     2881 aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt 
     2941 tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt 
     3001 agaaaaacaa cttaaatgtg aaagtgggtc ttaaaagcag cataaccttt ttccgtgatg 
     3061 gtaacttcag catgcgtttt agcgtttatt tcgtttagtt atcggcataa tcgttaaaac 
     3121 aggcgttatc gtagcgtaaa agcccttgag cgtagcgtgg ctttgcagcg aagatgttgt 
     3181 ctgttagatt atgaaagccg atgactgaat gaaataataa gcgcagcgcc cttctatttc 
     3241 ggttggagga ggctcaaggg agtatgaggg aatgaaattc cctcatgggt ttgattttaa 
     3301 aaattgcttg caattttgcc gagcggtagc gctggaaaat ttttgaaaaa aatttggaat 
     3361 ttggaaaaaa atggggggaa aggaagcgaa ttttgcttcc gtactacgac cccccattaa 
     3421 gtgccgagtg ccaatttttg tgccaaaaac gctctatccc aactggctca agggtttaag 
     3481 gggtttttca atcgccaacg aatcgccaac gttttcgcca acgtttttta taaatctata 
     3541 tttaagtagc tttattgttg tttttatgat tacaaagtga tacactaact ttataaaatt 
     3601 atttgattgg agttttttaa atggtgattt cagaatcgaa aaaaagagtt atgatttctc 
     3661 tgacaaaaga gcaagataaa aaattaacag atatggcgaa acaaaaaggt ttttcaaaat 
     3721 ctgcggttgc ggcgttagct atagaagaat atgcaagaaa ggaatcagaa caaaaaaaat 
     3781 aagcgaaagc tcgcgttttt agaaggatac gagttttcgc tacttgtttt tgataaggta 
     3841 attatatcat ggctattaaa aatactaaag ctagaaattt tggattttta ttatatcctg 
     3901 actcaattcc taatgattgg aaagaaaaat tagagagttt gggcgtatct atggctgtca 
     3961 gtcctttaca cgatatggac gaaaaaaaag ataaagatac atggaataat agtaatatta 
     4021 tacaaaatgg aaagcactat aaaaaaccac actatcacgt tatatatatt gcacgaaatc 
     4081 ctgtaacaat agaaagcgtt aggaacaaga ttaagcgaaa attggggaat agttcagttg 
     4141 ctcatgttga gatacttgat tatatcaaag gttcatatga atatttgact catgaatcaa 
     4201 aggacgctat tgctaagaat aaacatatat acgacaaaaa agatattttg aacattaatg 
     4261 attttgatat tgaccgctat ataacacttg atgaaagcca aaaaagagaa ttgaagaatt 
     4321 tacttttaga tatagtggat gactataatt tggtaaatac aaaagattta atggctttta 
     4381 ttcgccttag gggagcggag tttggaattt taaatacgaa tgatgtaaaa gatattgttt 
     4441 caacaaactc tagcgccttt agattatggt ttgagggcaa ttatcagtgt ggatatagag 
     4501 caagttatgc aaaggttctt gatgctgaaa cgggggaaat aaaatgacaa acaaagaaaa 
     4561 agagttattt gctgaaaatg aggaattaaa aaaagaaatt aaggacttaa aagagcgtat 
     4621 tgaaagatac agagaaatgg aagttgaatt aagtacaaca atagatttat tgagaggagg 
     4681 gattattgaa taaataaaag ccccctgacg aaagtcgaag ggggttttta ttttggtttg 
     4741 atgttgcgat taatagcaat acattctata atagaaggta tggaggatgt tatataatga 
     4801 gacagaatta tgatgatcat atgtcaacta acggggcagg ttagtgacat tagaaaaccg 
     4861 actgtaaaaa gtacagtcgg cattatctca tattattttt gacaccagac caactggtaa 
     4921 tggtagcgac cggcgctcag ctgtaattcc gccgatactg acgggctcca ggagtcgtcg 
     4981 ccaccaatcc ccatatggaa accgtcgata ttcagccatg tgccttcttc cgcgtgcagc 
     5041 agatggcgat ggctggtttc catcagttgc tgttgactgt agcggctgat gttgaactgg 
     5101 aagtcgccgc gccactggtg tgggccataa ttcaattcgc gcgtcccgca gcgcagaccg 
     5161 ttttcgctcg ggaagacgta cggggtatac atgtctgaca atggcagatc ccagcggtca 
     5221 aaacaggcgg cagtaaggcg gtcgggatag ttttcttgcg gccctaatcc gagccagttt 
     5281 acccgctctg ctacctgcgc cagctggcag ttcaggccaa tccgcgccgg atgcggtgta 
     5341 tcgctcgcca cttcaacatc aacggtaatc gccatttgac cactaccatc aatccggtag 
     5401 gttttccggc tgataaataa ggttttcccc tgatgctgcc acgcgtgagc ggtcgtaatc 
     5461 agcaccgcat cagcaagtgt atctgccgtg cactgcaaca acgctgcttc ggcctggtaa 
     5521 tggcccgccg ccttccagcg ttcgacccag gcgttagggt caatgcgggt cgcttcactt 
     5581 acgccaatgt cgttatccag cggtgcacgg gtgaactgat cgcgcagcgg cgtcagcagt 
     5641 tgttttttat cgccaatcca catctgtgaa agaaagcctg actggcggtt aaattgccaa 
     5701 cgcttattac ccagctcgat gcaaaaatcc atttcgctgg tggtcagatg cgggatggcg 
     5761 tgggacgcgg cggggagcgt cacactgagg ttttccgcca gacgccactg ctgccaggcg 
     5821 ctgatgtgcc cggcttctga ccatgcggtc gcgttcggtt gcactacgcg tactgtgagc 
     5881 cagagttgcc cggcgctctc cggctgcggt agttcaggca gttcaatcaa ctgtttacct 
     5941 tgtggagcga catccagagg cacttcaccg cttgccagcg gcttaccatc cagcgccacc 
     6001 atccagtgca ggagctcgtt atcgctatga cggaacaggt attcgctggt cacttcgatg 
     6061 gtttgcccgg ataaacggaa ctggaaaaac tgctgctggt gttttgcttc cgtcagcgct 
     6121 ggatgcggcg tgcggtcggc aaagaccaga ccgttcatac agaactggcg atcgttcggc 
     6181 gtatcgccaa aatcaccgcc gtaagccgac cacgggttgc cgttttcatc atatttaatc 
     6241 agcgactgat ccacccagtc ccagacgaag ccgccctgta aacggggata ctgacgaaac 
     6301 gcctgccagt atttagcgaa accgccaaga ctgttaccca tcgcgtgggc gtattcgcaa 
     6361 aggatcagcg ggcgcgtctc tccaggtagc gaaagccatt ttttgatgga ccatttcggc 
     6421 acagccggga agggctggtc ttcatccacg cgcgcgtaca tcgggcaaat aatatcggtg 
     6481 gccgtggtgt cggctccgcc gccttcatac tgcaccgggc gggaaggatc gacagatttg 
     6541 atccagcgat acagcgcgtc gtgattagcg ccgtggcctg attcattccc cagcgaccag 
     6601 atgatcacac tcgggtgatt acgatcgcgc tgcaccattc gcgttacgcg ttcgctcatc 
     6661 gccggtagcc agcgcggatc atcggtcaga cgattcattg gcaccatgcc gtgggtttca 
     6721 atattggctt catccaccac atacaggccg tagcggtcgc acagcgtgta ccacagcgga 
     6781 tggttcggat aatgcgaaca gcgcacggcg ttaaagttgt tctgcttcat cagcaggata 
     6841 tcctgcacca tcgtctgctc atccatgacc tgaccatgca gaggatgatg ctcgtgacgg 
     6901 ttaacgcctc gaatcagcaa cggcttgccg ttcagcagca gcagaccatt ttcaatccgc 
     6961 acctcgcgga aaccgacatc gcaggcttct gcttcaatca gcgtgccgtc ggcggtgtgc 
     7021 agttcaacca ccgcacgata gagattcggg atttcggcgc tccacagttt cgggttttcg 
     7081 acgttcagac gtagtgtgac gcgatcggca taaccaccac gctcatcgat aatttcaccg 
     7141 ccgaaaggcg cggtgccgct ggcgacctgc gtttcaccct gccataaaga aactgttacc 
     7201 cgtaggtagt cacgcaactc gccgcacatc tgaacttcag cctccagtac agcgcggctg 
     7261 aaatcatcat taaagcgagt ggcaacatgg aaatcgctga tttgtgtagt cggtttatgc 
     7321 agcaacgaga cgtcacggaa aatgccgctc atccgccaca tatcctgatc ttccagataa 
     7381 ctgccgtcac tccagcgcag caccatcacc gcgaggcggt tttctccggc gcgtaaaaat 
     7441 gcgctcaggt caaattcaga cggcaaacga ctgtcctggc cgtaaccgac ccagcgcccg 
     7501 ttgcaccaca gatgaaacgc cgagttaacg ccatcaaaaa taattcgcgt ctggccttcc 
     7561 tgtagccagc tttcatcaac attaaatgtg agcgagtaac aacccgtcgg attctccgtg 
     7621 ggaacaaacg gcggattgac cgtaatggga taggtcacgt tggtgtagat gggcgcatcg 
     7681 taaccgtgca tctgccagtt tgaggggacg acgacagtat cggcctcagg aagatcgcac 
     7741 tccagccagc tttccggcac cgcttctggt gccggaaacc aggcaaagcg ccattcgcca 
     7801 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag 
     7861 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccgg 
     7921 tcgacccgta atcttacgtc agtaacttcc atggatatcc tcctttataa aagccagtca 
     7981 ttaggcctat ctgacaattc ctgaatagag ttcataaaca atcctgcatg ataaccatca 
     8041 caaacagaat gatgtacctg taaagatagc ggtaaatata ttgaattacc tttattaatg 
     8101 aattttcctg ctgtaataat gggtagaagg taattactat tattattgat atttaagtta 
     8161 aacccagtaa atgaagtcca tggaataata gaaagagaaa aagcattttc aggtataggt 
     8221 gttttgggaa acaatttccc cgaaccatta tatttctcta catcagaaag gtataaatca 
     8281 taaaactctt tgaagtcatt ctttacagga gtccaaatac cagagaatgt tttagataca 
     8341 ccatcaaaaa ttgtataaag tggctctaac ttatcccaat aacctaactc tccgtcgcta 
     8401 ttgtaaccag ttctaaaagc tgtatttgag tttatcaccc ttgtcactaa gaaaataaat 
     8461 gcagggtaaa atttatatcc ttcttgtttt atgtttcggt ataaaacact aatatcaatt 
     8521 tctgtggtta tactaaaagt cgtttgttgg ttcaaataat gattaaatat ctcttttctc 
     8581 ttccaattgt ctaaatcaat tttattaaag ttcatgggtt tcactctcct tctacatttt 
     8641 ttaacctaat aatgccaaat accgtttgcc acccctctct ttgataatta taatattggc 
     8701 gaaattcgct tctaaagatg aaacgcaata ttatatgctt gctttatcgg ccgtatgtga 
     8761 ttataccagc cccctcacta catgtcaaga ataaactgcc aaagcataat gggat 
//
