LOCUS       pEX18-pheS-km           6755 bp ss-DNA     circular SYN 20-AUG-2018
DEFINITION  -.
ACCESSION   -
KEYWORDS    -.
SOURCE      
  ORGANISM  Francisella tularensis subsp. novicida U112.
COMMENT     enced.fa - Features not parsed: PCR_primer 6607..6626
            /note="JCOL171" PCR_primer 324..343 /note="JCOL172" PCR_primer
            complement(2030..2047) /note="JCOL173" PCR_primer
            complement(1346..1365) /note="JCOL174" PCR_primer 2252..2271
            /note="JCOL175" PCR_primer 3048..3066 /note="JCOL176" PCR_primer
            3724..3742 /note="JCOL177" PCR_primer 4356..4375 /note="JCOL178"
            PCR_primer 4996..5015 /note="JCOL179" PCR_primer 5655..5674
            /note="JCOL180" PCR_primer complement(3810..3828) /note="JCOL184"
            PCR_primer 3128..3147 /note="JCOL185".
FEATURES             Location/Qualifiers
     rep_origin      join(complement(1..288),complement(6461..6))
                     /note="ColE origin"
     promoter        866..1103
                     /note="groES promotoer"
                     /modified_by="User"
                     /label="groES promoter"
     gene            1104..1919
                     /created_by="User"
                     /Note="kanamycin resistance"
                     /modified_by="User"
                     /label="aphA1"
     promoter        2713..3024
                     /note="Francisella novicida U112 bfr promoter"
                     /modified_by="User"
                     /label="bfr promoter"
     gene            3022..4035
                     /gene="Francisella novicida U112 pheS (A305G)"
                     /modified_by="User"
                     /label="F. novicida U112 pheS (A305G)"
     unsure          6000..6038
                     /created_by="User"
                     /modified_by="User"
                     /label="MCS"
ORIGIN      
        1 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
       61 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
      121 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
      181 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
      241 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
      301 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
      361 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
      421 tcaatctaaa gtatatatga gtaaacttgg tctgacaatc gatcaagggt tggtttgcgc
      481 attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc
      541 gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac
      601 gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg
      661 ctcgccgagg cggcataaat cgccgtgacg atcagcggtc caatgatcga agttaggctg
      721 gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac
      781 agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg
      841 aaggccatcc agcctcgcgt cgatctatac ccttcaagct ttgaaaaata aacttaatta
      901 ttatatatgt tatttagcta gtttttttaa ttaaagttaa aatcgagagc ttgtttgaca
      961 aaaaaacaaa aaaatttctt gaaaattttt tttttgactc aatatctaga cttgcaagag
     1021 cttggaactt tgagattgtt ctaagatgca tacaaattca aaatgcttaa acaaaaataa
     1081 tttaacaaag gagtaagatt gttatgagcc atattcaacg ggaaacgtct tgctcgaggc
     1141 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
     1201 tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt
     1261 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     1321 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     1381 atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat
     1441 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     1501 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc
     1561 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct
     1621 ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag
     1681 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     1741 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     1801 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     1861 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat
     1921 cagaattggt taattggttg taacactggc agagcattac gctgacttga cgggacggcg
     1981 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
     2041 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
     2101 caaagctctc atcaacgata tcccgcaaga ggcccggcag taccggcata accaagccta
     2161 tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca
     2221 tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagctaat
     2281 cggttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     2341 tctcatgagc ggatacatat ttgaatgtat ttagagaaat aaacaaatag gggttccgcg
     2401 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     2461 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     2521 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     2581 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     2641 ctatgcggca tcagagcaga ttgtactgag agtgcaccat aatcggcatt ttcttttgcg
     2701 tttttagcta gcgatccata cccatgatgg ttattattgc gatcatcaca atattaaaat
     2761 taattttctt catttatttt tcttaaatat tattattaaa aatagtaaat ttaacttatc
     2821 taaaaatagc ataatatcat ttttattaaa atatctaggt tgaattctta gatattttga
     2881 tagatagata attagatact aaattgataa cttataaaga attaaatttt cttttgtatg
     2941 ttaacttgat tgctaatatg aattatatta gttagtatgt tgattataat gattagagtt
     3001 ttaaataatg gagataataa tatgcaaata gtcgagcaaa tgaaagataa agctcttgct
     3061 gagctaaatc ttgtcaaaga taaaaaaact ttagatgata ttcgagtaaa gtacctgggt
     3121 aaaaagggtg aattaactga gatgatgaag ttaatagcga cattacctaa tgatgagaaa
     3181 ccaaagctag gacaagcagt aaatattgca aaacaagctt tacaagaagc gatcaattta
     3241 aagctagcta attttgaaga gcaagaatta aatgaaaaac tagctcagga aaagattgat
     3301 ataacactaa gtggagtagg gcaaaatcaa ggctcacttc atcctgtaac aaaaacactt
     3361 aatcgaattg aagcattttt taaacagaat ggttttgcta ttgaatttgg tcctgagatt
     3421 gaaagtgatt attataattt tgaaacttta aatatccctt ctcatcatcc agctcgcgct
     3481 atgcatgata ctttttatat tgatgagact catgttttga gaacgcacac ctctggagtg
     3541 caaattagaa ctatggaaaa acaacagcca ccaattagaa taattgcgcc aggtagggtt
     3601 tatcgttgtg attcggatat tactcataca ccgatgtttc atcaagtaga aggtttactt
     3661 gtagataagg atgtttcttt tgctgatctt aaaggcttgt tgcatgcttt tcttaactca
     3721 ttttttgaaa aagatctaaa agtaagattt agaccatctt atttcccatt tactgagcct
     3781 tcagcagagg ctgatatcga atgtgtaatg tgtgatggta aaggttgtag agtatgtaaa
     3841 catactggtt ggttagaggt acttggttgc ggtatggtac atccaaaagt tttaaaagct
     3901 ggaaatgtag accctgaaaa atatcaagga tttggttttg gtatgggagt agaaagatta
     3961 tctatgctta gatatggtat agatgaccta agaatgttct ttgaaaatga tctaagattt
     4021 ttaaaacagt tttaacatat gcgttcatgt ctcctttttt atgtactgtg ttagcggtct
     4081 gcttcttcca gccctcctgt ttgaagatgg caagttagtt acgcacaata aaaaaagacc
     4141 taaaatatgt aaggggtgac gccaaagtat acactttgcc ctttacacat tttaggtctt
     4201 gcctgcttta tcagtaacaa acccgcgcga tttacttttc gacctcattc tattagactc
     4261 tcgtttggat tgcaactggt ctattttcct cttttgtttg atagaaaatc ataaaaggat
     4321 ttgcagacta cgggcctaaa gaactaaaaa atctatctgt ttcttttcat tctctgtatt
     4381 ttttatagtt tctgttgcat gggcataaag ttgccttttt aatcacaatt cagaaaatat
     4441 cataatatct catttcacta aataatagtg aacggcaggt atatgtgatg ggttaaaaag
     4501 gatcgatcct ctagctagag tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga
     4561 accgcgccgt gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca
     4621 ggtcgccatt gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt
     4681 tgtagccctg gccgacggcc agcaggtagg ccgacaggct catgccggct gccgccgcct
     4741 tttcctcaat cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct
     4801 tcctggttgg cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag
     4861 ccggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag
     4921 tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc
     4981 gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga
     5041 aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg
     5101 aaaagcgctg cttccctgct gttttgtgga atatctaccg actggaaaca ggcaaatgca
     5161 ggaaattact gaactgaggg gacaggcgag agacgatgcc aaagagctac accgacgagc
     5221 tggccgagtg ggttgaatcc cgcgcggcca agaagcgccg gcgtgatgag gctgcggttg
     5281 cgttcctggc ggtgagggcg gatgtcgata tgcggtgtga aataccgcac agatgcgtaa
     5341 ggagaaaata ccgcatcagg cgcatatttg aatgtattta gaaaaataaa caaaagagtt
     5401 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttaatttg atgcctggca
     5461 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcgcaac gttcaaatcc
     5521 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
     5581 aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggcagt tccctactct
     5641 cgcatgggga gaccccacac taccatcggc gctacggcgt ttcacttctg agttcggcat
     5701 ggggtcaggt gggaccaccg cgctactgcc gccaggcaaa ttctgtttta tcagaccgct
     5761 tctgcgttct gatttaatct gtatcaggct gaaaatcttc tctcatccgc caaaacagcc
     5821 aagctcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     5881 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     5941 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc
     6001 tgcaggtcga ctctagagga tccccgggta ccgagctcga attcgtaatc atgtcatagc
     6061 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6121 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6181 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6241 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgacccgc
     6301 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6361 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6421 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6481 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6541 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6601 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6661 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6721 ccgttcagcc cgaccgctgc gccttatccg gtaac
//
