LOCUS Exported 5435 bp ds-DNA circular SYN 15-APR-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 5435) AUTHORS Kathryn Ramsey TITLE Direct Submission JOURNAL Exported May 10, 2019 from SnapGene 4.1.9 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5435 /organism="recombinant plasmid" /mol_type="other DNA" primer_bind 4..26 /label=P025 pEX18KanSeq1F /note="P025 pEX18KanSeq1F" primer_bind 474..493 /label=P037 pEX18KanSeq9F /note="P037 pEX18KanSeq9F" primer_bind 733..755 /label=P026 pEX18KanSeq2F /note="P026 pEX18KanSeq2F" primer_bind 826..859 /label=P261 groESpCheck1 /note="P261 groESpCheck1" misc_feature 859..883 /label=P049 groES promoter F /note="P049 groES promoter F" promoter 866..1103 /note="groES promotoer" CDS 1104..1916 /label=kanamycin resistance /note="kanamycin resistance" misc_feature 1259..1283 /label=nonstandard type: PCR_primer /note="P442 pEX_kanR_F" misc_feature complement(1531..1553) /label=nonstandard type: PCR_primer /note="P443 pEX_kanR_R" primer_bind complement(1583..1607) /label=P027 pEX18KanSeq3F /note="P027 pEX18KanSeq3F" misc_feature complement(2007..2030) /label=nonstandard type: PCR_primer /note="P441 pEX_ckR" CDS complement(2120..2383) /label=sacB /note="sacB" primer_bind 2648..2674 /label=P031 pEX18KanSeq7F /note="P031 pEX18KanSeq7F" primer_bind 3367..3391 /label=P033 pEX18KanSeq8F /note="P033 pEX18KanSeq8F" primer_bind 4266..4289 /label=P023 pEX18SeqF2 /note="P023 pEX18SeqF2" primer_bind 4290..4309 /label=P021 pEX18SeqF1 /note="P021 pEX18SeqF1" primer_bind 4290..4309 /label=KROL6 Seq_pEX_1 primer_bind 4369..4401 /label=KROL157 FTL1548fragF gene 4375..4673 /label=FTL_1548 CDS 4375..4673 /codon_start=1 /label=hypothetical protein /translation="GDFWMYVKDFKDAEFPNKDYPILQTYADTFMTGCLQTQAEFNLTE FGKLCFDTTYNWDLANWLYDRSNPRYSRYSQDTEKYRPQIDKIIRRLTFDDDPL" primer_bind complement(4647..4685) /label=KROL158 FTL1548fragR primer_bind complement(4648..4673) /label=P813 CompFTL1548_1Ra /note="Contains BamHI site; F. tularensis LVS" primer_bind complement(4648..4673) /label=P813 CompFTL1548_1R misc_feature 4668..4673 /label=DFTL_1548_FR2 primer_bind complement(4676..4718) /label=P001 FTL_1743fragR /note="P001 FTL_1743fragR" sig_peptide 4683..4718 /note="VSVG" primer_bind complement(4687..4715) /label=P035 pEX18integrationVSVG /note="P035 pEX18integrationVSVG" primer_bind 4690..4719 /label=P226 SP_pKL02_F /note="P226 SP_pKL02_F" primer_bind complement(4739..4764) /label=P024 pEX18SeqR2 /note="P024 pEX18SeqR2" primer_bind complement(4761..4780) /label=P022 pEX18SeqR1 /note="P022 pEX18SeqR1" primer_bind complement(4761..4780) /label=KROL7 Seq_pEX_2 primer_bind complement(4899..4922) /label=P227 SP_pKL02_R /note="P227 SP_pKL02_R" rep_origin complement(join(5135..5435,1..288)) /direction=LEFT /label=ColE origin /note="ColE origin" ORIGIN 1 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 61 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 121 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 181 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 241 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 301 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 361 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 421 tcaatctaaa gtatatatga gtaaacttgg tctgacaatc gatcaagggt tggtttgcgc 481 attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc 541 gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac 601 gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg 661 ctcgccgagg cggcataaat cgccgtgacg atcagcggtc caatgatcga agttaggctg 721 gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac 781 agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg 841 aaggccatcc agcctcgcgt cgatctatac ccttcaagct ttgaaaaata aacttaatta 901 ttatatatgt tatttagcta gtttttttaa ttaaagttaa aatcgagagc ttgtttgaca 961 aaaaaacaaa aaaatttctt gaaaattttt tttttgactc aatatctaga cttgcaagag 1021 cttggaactt tgagattgtt ctaagatgca tacaaattca aaatgcttaa acaaaaataa 1081 tttaacaaag gagtaagatt gttatgagcc atattcaacg ggaaacgtct tgctcgaggc 1141 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg 1201 tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt 1261 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa 1321 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg 1381 atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat 1441 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt 1501 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc 1561 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct 1621 ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag 1681 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag 1741 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat 1801 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta 1861 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat 1921 cagaattggt taattggttg taacactggc agagcattac gctgacttga cgggacggcg 1981 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga 2041 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa 2101 caaagctctc atcaacgatc cagctgtccc aaacgtccag gccttttgca gaagagatat 2161 ttttaattgt ggacgaatcg aactcaggaa cttgatattt ttcatttttt tgctgttcag 2221 ggatttgcag catatcatgg cgtgtaatat gggaaatgcc gtatgtttcc ttatatggct 2281 tttggttcgt ttctttcgca aacgcttgag ttgcgcctcc tgccagcagt gcggtagtaa 2341 aggttaatac tgttgcttgt tttgcaaact ttttgatgtt catcgttcat gtctcctttt 2401 ttatgtactg tgttagcggt ctgcttcttc cagccctcct gtttgaagat ggcaagttag 2461 ttacgcacaa taaaaaaaga cctaaaatat gtaaggggtg acgccaaagt atacactttg 2521 ccctttacac attttaggtc ttgcctgctt tatcagtaac aaacccgcgc gatttacttt 2581 tcgacctcat tctattagac tctcgtttgg attgcaactg gtctattttc ctcttttgtt 2641 tgatagaaaa tcataaaagg atttgcagac tacgggccta aagaactaaa aaatctatct 2701 gtttcttttc attctctgta ttttttatag tttctgttgc atgggcataa agttgccttt 2761 ttaatcacaa ttcagaaaat atcataatat ctcatttcac taaataatag tgaacggcag 2821 gtatatgtga tgggttaaaa aggatcgatc ctctagctag agtcgatctt cgccagcagg 2881 gcgaggatcg tggcatcacc gaaccgcgcc gtgcgcgggt cgtcggtgag ccagagtttc 2941 agcaggccgc ccaggcggcc caggtcgcca ttgatgcggg ccagctcgcg gacgtgctca 3001 tagtccacga cgcccgtgat tttgtagccc tggccgacgg ccagcaggta ggccgacagg 3061 ctcatgccgg ctgccgccgc cttttcctca atcgctcttc gttcgtctgg aaggcagtac 3121 accttgatag gtgggctgcc cttcctggtt ggcttggttt catcagccat ccgcttgccc 3181 tcatctgtta cgccggcggt agccggccag cctcgcagag caggattccc gttgagcacc 3241 gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg gcctacttca 3301 cctatcctgc ccggctgacg ccgttggata caccaaggaa agtctacacg aaccctttgg 3361 caaaatcctg tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc tataatgacc 3421 ccgaagcagg gttatgcagc ggaaaagcgc tgcttccctg ctgttttgtg gaatatctac 3481 cgactggaaa caggcaaatg caggaaatta ctgaactgag gggacaggcg agagacgatg 3541 ccaaagagct acaccgacga gctggccgag tgggttgaat cccgcgcggc caagaagcgc 3601 cggcgtgatg aggctgcggt tgcgttcctg gcggtgaggg cggatgtcga tatgcggtgt 3661 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgcatatt tgaatgtatt 3721 tagaaaaata aacaaaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt 3781 ctgcttaatt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg 3841 ttgcttcgca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac 3901 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg 3961 atgcctggca gttccctact ctcgcatggg gagaccccac actaccatcg gcgctacggc 4021 gtttcacttc tgagttcggc atggggtcag gtgggaccac cgcgctactg ccgccaggca 4081 aattctgttt tatcagaccg cttctgcgtt ctgatttaat ctgtatcagg ctgaaaatct 4141 tctctcatcc gccaaaacag ccaagctcgc cattcgccat tcaggctgcg caactgttgg 4201 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct 4261 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg 4321 gccagtgcca agcttgcatg cctgcaggtc gactctagag gatccccggg taccaaggcg 4381 atttttggat gtatgttaaa gattttaaag atgccgaatt tccaaataaa gactatccta 4441 tcttacaaac atatgctgat acttttatga caggatgtct acaaactcaa gccgaattta 4501 acctaactga atttggtaaa ctttgttttg atacaaccta taactgggat ctagcaaact 4561 ggttatatga ccgttcaaac cctcgctata gcaggtattc acaagatact gagaaatatc 4621 gtccacaaat tgataaaatc attcgccgct taacttttga tgatgatcct ttagcggccg 4681 catatactga tattgaaatg aatagattag gtaaataaga attcgtaatc atgtcatagc 4741 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 4801 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 4861 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 4921 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgacccgc 4981 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 5041 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 5101 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 5161 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 5221 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 5281 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 5341 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 5401 ccgttcagcc cgaccgctgc gccttatccg gtaac //