LOCUS       Exported                7428 bp ds-DNA     circular SYN 24-MAR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Kathryn Ramsey
  TITLE     Direct Submission
  JOURNAL   Exported Mar 28, 2019 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          4754..5375
                     /note="/dnas_title=pF_nat+MglA-VSVG"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        299..532
                     /note="GroE"
     primer_bind     435..457
                     /label=AROL2
     primer_bind     complement(688..710)
                     /label=AROL3
     gene            853..1728
                     /label=ORF3
                     /note="ORF3"
     gene            complement(1951..2370)
                     /label=ORF2
                     /note="ORF2"
     gene            complement(2376..3392)
                     /label=RepA
                     /note="RepA"
     gene            4771..5343
                     /label=nat resistance
                     /note="nat resistance"
     CDS             5628..6488
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6659..7247
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccttgta tggattagtc gagctaaaaa gctcatattt tttatattca aactatatcc
      301 cttcaagctt tgaaaaataa acttaattat tatatatgtt atttagctag tttttttaat
      361 taaagttaaa atcgagagct tgtttgacaa aaaaacaaaa aaatttcttg aaaatttttt
      421 ttttgactca atatctagac ttgcaagagc ttggaacttt gagattgttc taagatgcat
      481 acaaattcaa aatgcttaaa caaaaataat ttaacaaagg agtaagattg ttatgaacat
      541 tcgtccatta caagatagag tattagttcg tcgtgcaggt accatatgaa ttcccatcgg
      601 accgttccaa cttaccgacc agttcggcag gtatgtattt gcgtgcattc ctatccaaaa
      661 aaacatcaag ccaaaagctt gaaaaaactt acaacacagc tcaacagagc tagattgtaa
      721 aaccctgctt tgttaagcag aacgcaaaaa ttgaatgact tatagtcata tcgcttcgac
      781 cctcgtagat tagtagcctt gagctattaa ctggttgaaa cacttaccaa ataaagatta
      841 aaagcgataa aaatgaaaga taaagcagct aaaaacagag attttagaaa gactatttta
      901 tcagtgttac aacgcaataa agatggctct tttgctacgc aagcaaatag aaagtctatt
      961 ctgttgcagg caactaaaga ccttaaaaag gtagggttta gcaaggttac agccgaaaac
     1021 ttcggtaata agcattgcta tgcacttaga gaccattgga gagcccaagg attagctaca
     1081 gcaacgataa aaaatcgttt agcttgtcta aggtggttag gcgagaaaat gggcaaagaa
     1141 ctacccgata atcgaaaatt agagattgag aacaggaagt atagcgataa ttcaatcaat
     1201 aaagcccaag aaatcgattt taaggcgatt tctgccttaa ctgataggca agccctagca
     1261 atacaattac agcgcgaatt tgggcttcgt agagaagaaa gtttgaagtt tcagcctagt
     1321 tatgcaatca aagagcataa aatcgagctt aaaagctctt ggacaaaggg tggaagacca
     1381 cgagaaatcc caattttgaa tgaaaaacag agagaattgt tagaaaaagt aaaagaggta
     1441 gcaggtaaag gctctctaat tgagagcgaa aagtcttata agcaagcaat ggaacatttc
     1501 acgactcgct gtcaaagagc agggattaag aatgttcatg gctttagaca tgcgtatgct
     1561 caagatagat ataggcaatt aacagggcgt gagtgtccta aaaatggtgg attaacatct
     1621 aagcagctaa cacctgagca aaagcaacaa gactatgaag ctagaatgac tattagtgaa
     1681 gagttaggtc atggtagaga agatgtaaca gtcaactact taggcagata aaaagcaata
     1741 tagctataga agaaaagaaa gctattttac atagtagatc gactcttctt agggatttta
     1801 tattttttga taaatcatct attttgctag ttaaatcatc aaatttatca tcttgttgtt
     1861 tgactaaatc taagaatcta ttctcttttt taaaatcgtt catgcaaacc gcctatagct
     1921 ttcttctttt tctgaaatta tttgtcttca caccataatt aaattcccat ttttataagt
     1981 aaagtctttt aaaagcttgt cagtctcttc tctagaaatg taccaaattt tacctatttt
     2041 aggatacttt tcatgaagct cttctatttt tccccagtcc tttaatagtc tacctttaga
     2101 gtctcgtaaa tagttatctt tgtgacaggg gcctctttta tcttttttaa tgtaactata
     2161 tgttattcca acgtcactat tactattatc caaatctttt ttagcatgcc agtaagaact
     2221 ttcataactt aactctatct ttcgacctct ttgatataca acaataaagc tatagccagt
     2281 agtaacaacc tgttttactt ttgttaaatc tattaacttc ttatttattt ttttatgttt
     2341 ttttgaaaat ttaaatattt ctatattcat tcctacactt cctcaaatcc aaatggtagc
     2401 ttatgattct cttctggttt cttttctaat ttttttatat ttgcaataaa aactcttttt
     2461 ctatctttga tttttttatt gtcccaattc ctccaagtat catcacaaac cctttcaata
     2521 tcatgtaaat gatgatgtct aaatattgat ctgacataat acagatctag gtctagttca
     2581 tcacttaaca caacttctct aagtctttca gatgcttcga ttggtatgta atcctcttta
     2641 tttttagtat ctaaaagctt ttgcttaaat tcttcttctg tctctgctac cttactaact
     2701 gtaaacttga tatttgtaat cttacgacca tgttttctgt gatgatcctt gtcatcatag
     2761 gttacaaaaa tatccgataa ttgattaatc tcttctagtg ctggtaatag gaacttattt
     2821 ttaaaatttg aatatctgtt gctgtaactt ttaggtaaat caaaatcatt aatcatatca
     2881 tcgacataca atacgcaatc aactatatta gcataccctg cttgttcgcc taatttgctt
     2941 ttgagaagta agtataatct gcttgaatac ttacttttaa atgaaaatag taactgtctt
     3001 tctgctttag taaagtactc ttgtagttgt atcatgtgtg gcattaatga ccaatgaaac
     3061 tcgcaaatta aagcactgct tttagggtct gcttcaatat atgcaaacca gttagctatc
     3121 ttcgtttgtt ctttattcag ccatactggc ttagacatta ttgagtgcat taattgcttc
     3181 aatctcactc tgttatgctt aacccctgta gctttttcaa gatcagatag gcttatctta
     3241 tacctgtgaa actctttatc ttctctttta accattgagg caactaagaa tattaagttt
     3301 tgttcttctt ttgtaaggct atactttcct gcaacaagag tattagacat agctatttct
     3361 ttgccagcat ttacattttt aacttctttc atagaactag agtcattatc tcgatataca
     3421 aattctataa aacttctatt agtaaaacaa ctacttcata aaaaaaagta gttttaacga
     3481 tacaaaaagt agttttaaat tcaaaaagtg atacaaaaag tagttttaaa ttcaaaaagt
     3541 gatacaaaaa gtagttttaa attttttaaa aaagtgcttc aaagccttat gtagcaatac
     3601 ttacagagga ttaaaaaaaa atctgacaat atataaagag aatatataaa gagaatatct
     3661 taggggattt taaaaaaatc ccacagactc aaagactttt ttgacttttt aaatcctaga
     3721 aactatactt taagtactta tttaagtaca tggatttaga ttatgcaaac cgttaattat
     3781 tcaactttta gaaatgaact atctgattca atggatagag taacaaaaaa tcatagtcct
     3841 atgattgtaa ctagaggttc aaaaaaagaa gcagttgtta tgatgtcgtt agaggattct
     3901 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     3961 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     4021 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4081 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     4141 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     4201 tgatttaaca aaaatttaac gcgaatttta acaaaattca gggcgcaagg gctgctaaag
     4261 gaagcggaac acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag
     4321 ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag
     4381 tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt
     4441 gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt
     4501 cttgccgcca aggatctgat ggcgcagggg atcaagatct gatcaagaga caggatgagg
     4561 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     4621 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     4681 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     4741 gaatgaactg caggtaggga ggattttatt atgtctacta ctttggatga tactgcttat
     4801 agatacagaa cttctgttcc aggtgatgct gaagctattg aagctttgga tggttctttc
     4861 actaccgata ctgtttttag agttactgct actggtgatg gtttcacttt gagagaagtt
     4921 ccagttgatc caccattgac taaggttttc ccagatgatg aatccgatga tgaatccgat
     4981 gctggtgaag atggtgatcc agattctaga actttcgttg cttatggtga tgatggtgat
     5041 ttggctggtt tcgttgttgt ttcttattct ggttggaaca gaagattgac tgttgaagat
     5101 attgaagttg ctccagaaca tagaggtcat ggtgttggta gagctttgat gggtttggct
     5161 actgaattcg ccagagaaag aggtgctggt catttgtggt tggaagttac caatgttaat
     5221 gctccagcta ttcatgctta tagaagaatg ggtttcactt tgtgtggttt ggatactgct
     5281 ttatacgatg gtactgcttc cgatggtgaa caagctttgt atatgtctat gccatgtcca
     5341 taatgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     5401 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     5461 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     5521 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     5581 ttctatcgcc ttcttgacga gttcttctga attgaaaaag gaagagtatg agtattcaac
     5641 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5701 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5761 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5821 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5881 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5941 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     6001 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     6061 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     6121 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6181 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6241 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6301 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6361 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6421 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6481 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6541 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6601 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6661 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6721 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6781 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6841 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6901 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6961 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7021 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7081 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7141 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7201 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7261 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7321 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7381 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaag
//
