LOCUS       Exported                9043 bp ds-DNA     circular SYN 28-MAR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9043)
  AUTHORS   Jamie Wandzilak
  TITLE     Direct Submission
  JOURNAL   Exported Mar 28, 2019 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9043
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        299..532
                     /note="GroE"
     primer_bind     435..457
                     /label=AROL2
     primer_bind     598..639
                     /label=KROL96 FTL_0766F
     gene            617..2422
                     /label=FTL_0766
     CDS             617..2422
                     /codon_start=1
                     /label=gamma-glutamyltranspeptidase
                     /translation="MRRFLLSYTYSVFKNTLRLLVKKLFVNLMSLLILLPSLTLIAAPV
                     PTGYGFIPIQEEMQIAQPFTATHGMVSSQEALASKVGLDILKQGGNAVDAAVAVGFALA
                     VTLPRAGNLAGGGFMIIHLQDGNKNIAINYREKAPAKASRDMFLNDKGDIDYAKVSGSY
                     SASGVPGTVACLIDAQQKYGKLKLSQVIEPAIKLAEDGIPVSYDLHQSLVTAKPWLQKS
                     PDAMKIFYKKDGSAYEVGEILKQPELANSLKLIAKQGKKAFYEGEIAHKIADSMAKNGG
                     LITLQDLKNYNIEEMKPVKGTYRGYTIYSMPPPSSGGVILIELLNILENFPLSDYGNNS
                     AKTINLMSNAMSYAYNDRNSDLGDPDFVKMDLAKFLSKKYAKQIAQKITTDKHIPSKDI
                     STIDPDDHEKLQTTHFSIIDKDGNMVSNTYTLNYSYGSGIVVPGTGIFLNNEMDDFAAK
                     VGEANVFGLVQGEVNTVAPNKRPLSSMTPTIVLDNDGKPFLATGSPGGSRIITTTLQVI
                     LNIIDFNMNLQAAVNNPRIHSQLWPEEIGVEQGISVDTINLLKKMGNTVTPYAAMGAAE
                     SVMSDGQYVYGAADPRRASALAIGY"
     primer_bind     complement(2396..2429)
                     /label=KROL97 FTL_0766R
     primer_bind     complement(2548..2570)
                     /label=AROL3
     gene            2713..3588
                     /label=ORF3
                     /note="ORF3"
     gene            complement(3811..4230)
                     /label=ORF2
                     /note="ORF2"
     gene            complement(4236..5252)
                     /label=RepA
                     /note="RepA"
     CDS             6431..7225
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7243..8103
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8274..8862
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccttgta tggattagtc gagctaaaaa gctcatattt tttatattca aactatatcc
      301 cttcaagctt tgaaaaataa acttaattat tatatatgtt atttagctag tttttttaat
      361 taaagttaaa atcgagagct tgtttgacaa aaaaacaaaa aaatttcttg aaaatttttt
      421 ttttgactca atatctagac ttgcaagagc ttggaacttt gagattgttc taagatgcat
      481 acaaattcaa aatgcttaaa caaaaataat ttaacaaagg agtaagattg ttatgaacat
      541 tcgtccatta caagatagag tattagttcg tcgtgcaggt accatatgaa ttcccgggcg
      601 gccgcaggag gctttaatgc gtcgtttttt gttatcttat acttattctg tttttaaaaa
      661 tacactgagg ttattagtga aaaaattatt tgtaaactta atgtcattgt taatactgct
      721 accaagttta actctaattg ctgcaccagt ccctacaggt tatggtttta ttcctattca
      781 agaagagatg caaattgctc aaccttttac tgcaacacat ggtatggttt cttctcaaga
      841 agctctggca agtaaagtag gcttagatat cctaaagcaa ggtggtaatg ctgtagatgc
      901 agctgtagct gttggttttg ctttagctgt cacgctacca agagctggta atctcgctgg
      961 tggtggattt atgattatac atctacaaga tggcaataag aatatagcta taaactatcg
     1021 tgaaaaagct ccagcgaaag catctcgtga tatgtttcta aatgataaag gtgatattga
     1081 ttatgctaaa gtttctggtt catatagcgc atctggtgtt cctggtactg ttgcatgctt
     1141 aatcgatgca caacaaaaat atggcaaatt aaaactatca caagttattg aacctgctat
     1201 caaacttgct gaagatggta ttcctgtaag ctatgatctt caccagtcac ttgtaactgc
     1261 aaaaccatgg ctacaaaaat ctccagatgc gatgaaaatc ttttataaaa aagatggatc
     1321 agcctatgag gtaggagaaa tcctaaaaca gcctgaactt gctaatagtt taaagctaat
     1381 tgcaaaacaa ggcaaaaaag ctttctatga aggagaaata gctcataaga tagccgatag
     1441 tatggctaaa aatggtggtt taataacatt gcaagatttg aaaaattata atattgagga
     1501 gatgaagcca gttaagggaa cttatagagg ttatactata tattctatgc cgccgccaag
     1561 ttctggtggt gtgattctaa tagaattatt aaatattctg gagaattttc cattgtctga
     1621 ctatggtaac aatagtgcta agactattaa tcttatgagt aatgcgatga gctatgctta
     1681 taatgatagg aattctgact taggagatcc agattttgtc aaaatggatt tagctaagtt
     1741 tttatctaag aagtatgcaa aacagattgc tcaaaagata actacagata agcatatccc
     1801 tagtaaagat attagtacta tagatccgga tgatcatgaa aaattacaaa ctacacattt
     1861 tagtattatt gataaagatg gtaatatggt atcaaatact tataccttaa attattctta
     1921 tggaagtggt attgtagtac caggaacggg aattttttta aataacgaaa tggatgattt
     1981 cgctgctaaa gttggcgagg caaatgtctt tggtttggtt caaggtgaag taaatactgt
     2041 cgcaccgaat aaacgaccat taagttcaat gactccaacg atagtattag ataatgatgg
     2101 taaaccattt ttagctacag gttctcctgg aggaagtcgt attattacaa caacgttaca
     2161 agtgattcta aatatcattg attttaatat gaatcttcaa gcagcagtta ataatcctcg
     2221 tatacatagt caattatggc cagaagagat tggtgtagag cagggtatat ctgtagatac
     2281 aattaatcta cttaagaaga tgggtaacac tgtcacacct tatgctgcta tgggagctgc
     2341 tgaatctgta atgtcagatg gacaatatgt ctatggagct gcagatccac gcagagctag
     2401 cgcattagct ataggttatt aaggatccac tagctcgttt caaattaccg atgatatcgg
     2461 accgttccaa cttaccgacc agttcggcag gtatgtattt gcgtgcattc ctatccaaaa
     2521 aaacatcaag ccaaaagctt gaaaaaactt acaacacagc tcaacagagc tagattgtaa
     2581 aaccctgctt tgttaagcag aacgcaaaaa ttgaatgact tatagtcata tcgcttcgac
     2641 cctcgtagat tagtagcctt gagctattaa ctggttgaaa cacttaccaa ataaagatta
     2701 aaagcgataa aaatgaaaga taaagcagct aaaaacagag attttagaaa gactatttta
     2761 tcagtgttac aacgcaataa agatggctct tttgctacgc aagcaaatag aaagtctatt
     2821 ctgttgcagg caactaaaga ccttaaaaag gtagggttta gcaaggttac agccgaaaac
     2881 ttcggtaata agcattgcta tgcacttaga gaccattgga gagcccaagg attagctaca
     2941 gcaacgataa aaaatcgttt agcttgtcta aggtggttag gcgagaaaat gggcaaagaa
     3001 ctacccgata atcgaaaatt agagattgag aacaggaagt atagcgataa ttcaatcaat
     3061 aaagcccaag aaatcgattt taaggcgatt tctgccttaa ctgataggca agccctagca
     3121 atacaattac agcgcgaatt tgggcttcgt agagaagaaa gtttgaagtt tcagcctagt
     3181 tatgcaatca aagagcataa aatcgagctt aaaagctctt ggacaaaggg tggaagacca
     3241 cgagaaatcc caattttgaa tgaaaaacag agagaattgt tagaaaaagt aaaagaggta
     3301 gcaggtaaag gctctctaat tgagagcgaa aagtcttata agcaagcaat ggaacatttc
     3361 acgactcgct gtcaaagagc agggattaag aatgttcatg gctttagaca tgcgtatgct
     3421 caagatagat ataggcaatt aacagggcgt gagtgtccta aaaatggtgg attaacatct
     3481 aagcagctaa cacctgagca aaagcaacaa gactatgaag ctagaatgac tattagtgaa
     3541 gagttaggtc atggtagaga agatgtaaca gtcaactact taggcagata aaaagcaata
     3601 tagctataga agaaaagaaa gctattttac atagtagatc gactcttctt agggatttta
     3661 tattttttga taaatcatct attttgctag ttaaatcatc aaatttatca tcttgttgtt
     3721 tgactaaatc taagaatcta ttctcttttt taaaatcgtt catgcaaacc gcctatagct
     3781 ttcttctttt tctgaaatta tttgtcttca caccataatt aaattcccat ttttataagt
     3841 aaagtctttt aaaagcttgt cagtctcttc tctagaaatg taccaaattt tacctatttt
     3901 aggatacttt tcatgaagct cttctatttt tccccagtcc tttaatagtc tacctttaga
     3961 gtctcgtaaa tagttatctt tgtgacaggg gcctctttta tcttttttaa tgtaactata
     4021 tgttattcca acgtcactat tactattatc caaatctttt ttagcatgcc agtaagaact
     4081 ttcataactt aactctatct ttcgacctct ttgatataca acaataaagc tatagccagt
     4141 agtaacaacc tgttttactt ttgttaaatc tattaacttc ttatttattt ttttatgttt
     4201 ttttgaaaat ttaaatattt ctatattcat tcctacactt cctcaaatcc aaatggtagc
     4261 ttatgattct cttctggttt cttttctaat ttttttatat ttgcaataaa aactcttttt
     4321 ctatctttga tttttttatt gtcccaattc ctccaagtat catcacaaac cctttcaata
     4381 tcatgtaaat gatgatgtct aaatattgat ctgacataat acagatctag gtctagttca
     4441 tcacttaaca caacttctct aagtctttca gatgcttcga ttggtatgta atcctcttta
     4501 tttttagtat ctaaaagctt ttgcttaaat tcttcttctg tctctgctac cttactaact
     4561 gtaaacttga tatttgtaat cttacgacca tgttttctgt gatgatcctt gtcatcatag
     4621 gttacaaaaa tatccgataa ttgattaatc tcttctagtg ctggtaatag gaacttattt
     4681 ttaaaatttg aatatctgtt gctgtaactt ttaggtaaat caaaatcatt aatcatatca
     4741 tcgacataca atacgcaatc aactatatta gcataccctg cttgttcgcc taatttgctt
     4801 ttgagaagta agtataatct gcttgaatac ttacttttaa atgaaaatag taactgtctt
     4861 tctgctttag taaagtactc ttgtagttgt atcatgtgtg gcattaatga ccaatgaaac
     4921 tcgcaaatta aagcactgct tttagggtct gcttcaatat atgcaaacca gttagctatc
     4981 ttcgtttgtt ctttattcag ccatactggc ttagacatta ttgagtgcat taattgcttc
     5041 aatctcactc tgttatgctt aacccctgta gctttttcaa gatcagatag gcttatctta
     5101 tacctgtgaa actctttatc ttctctttta accattgagg caactaagaa tattaagttt
     5161 tgttcttctt ttgtaaggct atactttcct gcaacaagag tattagacat agctatttct
     5221 ttgccagcat ttacattttt aacttctttc atagaactag agtcattatc tcgatataca
     5281 aattctataa aacttctatt agtaaaacaa ctacttcata aaaaaaagta gttttaacga
     5341 tacaaaaagt agttttaaat tcaaaaagtg atacaaaaag tagttttaaa ttcaaaaagt
     5401 gatacaaaaa gtagttttaa attttttaaa aaagtgcttc aaagccttat gtagcaatac
     5461 ttacagagga ttaaaaaaaa atctgacaat atataaagag aatatataaa gagaatatct
     5521 taggggattt taaaaaaatc ccacagactc aaagactttt ttgacttttt aaatcctaga
     5581 aactatactt taagtactta tttaagtaca tggatttaga ttatgcaaac cgttaattat
     5641 tcaactttta gaaatgaact atctgattca atggatagag taacaaaaaa tcatagtcct
     5701 atgattgtaa ctagaggttc aaaaaaagaa gcagttgtta tgatgtcgtt agaggattct
     5761 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     5821 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     5881 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     5941 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     6001 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     6061 tgatttaaca aaaatttaac gcgaatttta acaaaattca gggcgcaagg gctgctaaag
     6121 gaagcggaac acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag
     6181 ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag
     6241 tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt
     6301 gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt
     6361 cttgccgcca aggatctgat ggcgcagggg atcaagatct gatcaagaga caggatgagg
     6421 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     6481 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     6541 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     6601 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     6661 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     6721 gccggggcag gatctcctgt catcccacct tgctcctgcc gagaaagtat ccatcatggc
     6781 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     6841 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     6901 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     6961 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     7021 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     7081 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     7141 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     7201 tcgccttctt gacgagttct tctgaattga aaaaggaaga gtatgagtat tcaacatttc
     7261 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     7321 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     7381 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     7441 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     7501 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     7561 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     7621 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     7681 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     7741 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     7801 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     7861 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     7921 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     7981 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     8041 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     8101 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     8161 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     8221 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     8281 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     8341 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     8401 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     8461 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     8521 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     8581 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     8641 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     8701 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     8761 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     8821 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     8881 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     8941 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     9001 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aag
//
