LOCUS       Exported                9025 bp ds-DNA     circular SYN 11-MAR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9025)
  AUTHORS   Kathryn Ramsey
  TITLE     Direct Submission
  JOURNAL   Exported Mar 28, 2019 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9025
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          6351..6972
                     /note="/dnas_title=pF_nat+MglA-VSVG"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        299..532
                     /note="GroE"
     primer_bind     435..457
                     /label=AROL2
     primer_bind     598..633
                     /label=KROL94 FTL_1251F
     gene            618..2159
                     /label=FTL_1251
     CDS             618..2159
                     /codon_start=1
                     /label=proton-dependent oligopeptide transport
                     /translation="VTISDVTHSKNNNITPFWVLWTLELWERFGYYGLQAILAIYFARK
                     LVFSDAEAMLIFGSFSALLYGGPLIGGWIGDSYLGAKRTIFIGAGILFLSYISLSFSTF
                     IAEYLGSTEKTVVMYSLAGVAIGGGLFKANPTSLISKLFEKGDPALDGAMTLYYMAINI
                     GSFVSMLLTPIVAAKYGYLHAFLCSAIGMFLGLISYTIFYPKIKRISTEAGRQKMSWFK
                     VFVVLLGACIAIFIIGNILENTTLCTSIVVVIAILALVYFFIQMLKQAEHERKRMIVAL
                     ILIIQGIIFFVLYQQMPTSLNFFAVNNVDPHFLGMNVHGEQWQVLNPLVIVLMSPILSV
                     VYKKIPGTHVTKFCFGMTLCALAFLVLYFPQFTATNGVVSGWWLVASYFFQSTGELLIS
                     ALGLSMVAELFPRNMSGLAQGMWLLTTMVAGPIGGYVGALTAPAAGTVFTKVEGLAVYG
                     HVFLTIGIFVAVIAAIMWIARGWLNAIIESTRVHFVSEGTHGMHEETAVLPTQNN"
     primer_bind     768..793
                     /label=KROL15 FTL1251fragF
     primer_bind     1048..1073
                     /label=KROL135 Ck1_FTL_1251
     primer_bind     complement(1182..1208)
                     /label=KROL16 FTL1251fragR
     primer_bind     1664..1691
                     /label=KROL136 Ck2_FTL_1251
     primer_bind     complement(2131..2166)
                     /label=KROL95 FTL_1251R
     gene            complement(2156..2159)
                     /label=FTL_1252
     CDS             complement(2156..2159)
                     /codon_start=1
                     /label=ATP-dependent RNA helicase RhlE
                     /translation=""
     primer_bind     complement(2285..2307)
                     /label=AROL3
     gene            2450..3325
                     /label=ORF3
                     /note="ORF3"
     gene            complement(3548..3967)
                     /label=ORF2
                     /note="ORF2"
     gene            complement(3973..4989)
                     /label=RepA
                     /note="RepA"
     gene            6368..6940
                     /label=nat resistance
                     /note="nat resistance"
     CDS             7225..8085
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8256..8844
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccttgta tggattagtc gagctaaaaa gctcatattt tttatattca aactatatcc
      301 cttcaagctt tgaaaaataa acttaattat tatatatgtt atttagctag tttttttaat
      361 taaagttaaa atcgagagct tgtttgacaa aaaaacaaaa aaatttcttg aaaatttttt
      421 ttttgactca atatctagac ttgcaagagc ttggaacttt gagattgttc taagatgcat
      481 acaaattcaa aatgcttaaa caaaaataat ttaacaaagg agtaagattg ttatgaacat
      541 tcgtccatta caagatagag tattagttcg tcgtgcaggt accatatgaa ttcccgggcg
      601 gccgcaggag aataattgtg actattagtg atgtgactca ttcgaaaaat aataatatta
      661 caccattttg ggttctttgg actttggagt tatgggagag atttggttat tatggtctac
      721 aagctatatt agctatatat tttgctagaa aattagtttt cagtgatgcc gaagcaatgt
      781 taatttttgg ttctttttcg gcattattat atggaggtcc attaatcggt ggttggattg
      841 gcgatagcta ccttggtgca aagcgtacaa tatttattgg cgctgggatt ttatttttat
      901 catatatatc acttagtttt agtacattta ttgccgagta tctaggcagt acagaaaaga
      961 cggttgttat gtactcacta gctggtgttg ctattggtgg gggtctcttt aaagctaacc
     1021 ctacttcttt aatctctaag ttatttgaaa aaggtgatcc tgctttagat ggggctatga
     1081 cactatatta tatggctatt aatattggtt cttttgtatc tatgcttctt acccctatag
     1141 tagcggctaa atatggttat cttcatgctt ttttatgttc agctattggg atgtttctag
     1201 gattaattag ctacactatt ttctatccaa aaattaaaag aatctcaaca gaagctggta
     1261 gacagaagat gtcttggttt aaggtttttg tggtactttt aggagcttgt atagcaatct
     1321 ttataatagg taatatactt gagaatacta ctttgtgtac tagtatagtt gttgtaatag
     1381 cgatacttgc tttggtatat ttttttatac aaatgttgaa acaagctgag catgaaagaa
     1441 aaaggatgat tgtagctttg atattaatta ttcaaggaat tatcttcttt gtattgtatc
     1501 agcaaatgcc tacttctctt aatttctttg cagttaataa tgtcgatcct cactttttag
     1561 gcatgaatgt acacggtgag caatggcagg tacttaatcc tctagtaata gtattaatgt
     1621 caccaattct atcggttgtg tataagaaaa tacctggaac tcatgtcaca aaattttgtt
     1681 ttggtatgac tttatgtgca ttagcatttt tagtactata ctttccacaa tttaccgcga
     1741 ctaatggtgt agtatctggt tggtggttag ttgcaagtta cttcttccaa tctactggtg
     1801 agcttctaat ctcagcatta ggtttatcta tggttgctga actattccca agaaatatga
     1861 gtggtcttgc acaaggtatg tggctactta ctacaatggt ggctggtcca attggtggtt
     1921 atgtaggtgc tttgacagct ccagcagcag gtactgtatt tacgaaggtc gaaggactag
     1981 ctgtttatgg tcatgttttt ttaactatag gtatttttgt cgctgtaatt gctgctatta
     2041 tgtggattgc tcgcggatgg ctaaatgcta ttatcgagag tactagagta cattttgtat
     2101 cagaaggtac tcatggtatg cacgaagaga ctgctgtttt acctacacaa aataattaag
     2161 gatccactag ctcgtttcaa attaccgatg atatcggacc gttccaactt accgaccagt
     2221 tcggcaggta tgtatttgcg tgcattccta tccaaaaaaa catcaagcca aaagcttgaa
     2281 aaaacttaca acacagctca acagagctag attgtaaaac cctgctttgt taagcagaac
     2341 gcaaaaattg aatgacttat agtcatatcg cttcgaccct cgtagattag tagccttgag
     2401 ctattaactg gttgaaacac ttaccaaata aagattaaaa gcgataaaaa tgaaagataa
     2461 agcagctaaa aacagagatt ttagaaagac tattttatca gtgttacaac gcaataaaga
     2521 tggctctttt gctacgcaag caaatagaaa gtctattctg ttgcaggcaa ctaaagacct
     2581 taaaaaggta gggtttagca aggttacagc cgaaaacttc ggtaataagc attgctatgc
     2641 acttagagac cattggagag cccaaggatt agctacagca acgataaaaa atcgtttagc
     2701 ttgtctaagg tggttaggcg agaaaatggg caaagaacta cccgataatc gaaaattaga
     2761 gattgagaac aggaagtata gcgataattc aatcaataaa gcccaagaaa tcgattttaa
     2821 ggcgatttct gccttaactg ataggcaagc cctagcaata caattacagc gcgaatttgg
     2881 gcttcgtaga gaagaaagtt tgaagtttca gcctagttat gcaatcaaag agcataaaat
     2941 cgagcttaaa agctcttgga caaagggtgg aagaccacga gaaatcccaa ttttgaatga
     3001 aaaacagaga gaattgttag aaaaagtaaa agaggtagca ggtaaaggct ctctaattga
     3061 gagcgaaaag tcttataagc aagcaatgga acatttcacg actcgctgtc aaagagcagg
     3121 gattaagaat gttcatggct ttagacatgc gtatgctcaa gatagatata ggcaattaac
     3181 agggcgtgag tgtcctaaaa atggtggatt aacatctaag cagctaacac ctgagcaaaa
     3241 gcaacaagac tatgaagcta gaatgactat tagtgaagag ttaggtcatg gtagagaaga
     3301 tgtaacagtc aactacttag gcagataaaa agcaatatag ctatagaaga aaagaaagct
     3361 attttacata gtagatcgac tcttcttagg gattttatat tttttgataa atcatctatt
     3421 ttgctagtta aatcatcaaa tttatcatct tgttgtttga ctaaatctaa gaatctattc
     3481 tcttttttaa aatcgttcat gcaaaccgcc tatagctttc ttctttttct gaaattattt
     3541 gtcttcacac cataattaaa ttcccatttt tataagtaaa gtcttttaaa agcttgtcag
     3601 tctcttctct agaaatgtac caaattttac ctattttagg atacttttca tgaagctctt
     3661 ctatttttcc ccagtccttt aatagtctac ctttagagtc tcgtaaatag ttatctttgt
     3721 gacaggggcc tcttttatct tttttaatgt aactatatgt tattccaacg tcactattac
     3781 tattatccaa atctttttta gcatgccagt aagaactttc ataacttaac tctatctttc
     3841 gacctctttg atatacaaca ataaagctat agccagtagt aacaacctgt tttacttttg
     3901 ttaaatctat taacttctta tttatttttt tatgtttttt tgaaaattta aatatttcta
     3961 tattcattcc tacacttcct caaatccaaa tggtagctta tgattctctt ctggtttctt
     4021 ttctaatttt tttatatttg caataaaaac tctttttcta tctttgattt ttttattgtc
     4081 ccaattcctc caagtatcat cacaaaccct ttcaatatca tgtaaatgat gatgtctaaa
     4141 tattgatctg acataataca gatctaggtc tagttcatca cttaacacaa cttctctaag
     4201 tctttcagat gcttcgattg gtatgtaatc ctctttattt ttagtatcta aaagcttttg
     4261 cttaaattct tcttctgtct ctgctacctt actaactgta aacttgatat ttgtaatctt
     4321 acgaccatgt tttctgtgat gatccttgtc atcataggtt acaaaaatat ccgataattg
     4381 attaatctct tctagtgctg gtaataggaa cttattttta aaatttgaat atctgttgct
     4441 gtaactttta ggtaaatcaa aatcattaat catatcatcg acatacaata cgcaatcaac
     4501 tatattagca taccctgctt gttcgcctaa tttgcttttg agaagtaagt ataatctgct
     4561 tgaatactta cttttaaatg aaaatagtaa ctgtctttct gctttagtaa agtactcttg
     4621 tagttgtatc atgtgtggca ttaatgacca atgaaactcg caaattaaag cactgctttt
     4681 agggtctgct tcaatatatg caaaccagtt agctatcttc gtttgttctt tattcagcca
     4741 tactggctta gacattattg agtgcattaa ttgcttcaat ctcactctgt tatgcttaac
     4801 ccctgtagct ttttcaagat cagataggct tatcttatac ctgtgaaact ctttatcttc
     4861 tcttttaacc attgaggcaa ctaagaatat taagttttgt tcttcttttg taaggctata
     4921 ctttcctgca acaagagtat tagacatagc tatttctttg ccagcattta catttttaac
     4981 ttctttcata gaactagagt cattatctcg atatacaaat tctataaaac ttctattagt
     5041 aaaacaacta cttcataaaa aaaagtagtt ttaacgatac aaaaagtagt tttaaattca
     5101 aaaagtgata caaaaagtag ttttaaattc aaaaagtgat acaaaaagta gttttaaatt
     5161 ttttaaaaaa gtgcttcaaa gccttatgta gcaatactta cagaggatta aaaaaaaatc
     5221 tgacaatata taaagagaat atataaagag aatatcttag gggattttaa aaaaatccca
     5281 cagactcaaa gacttttttg actttttaaa tcctagaaac tatactttaa gtacttattt
     5341 aagtacatgg atttagatta tgcaaaccgt taattattca acttttagaa atgaactatc
     5401 tgattcaatg gatagagtaa caaaaaatca tagtcctatg attgtaacta gaggttcaaa
     5461 aaaagaagca gttgttatga tgtcgttaga ggattcttcc cttcctttct cgccacgttc
     5521 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5581 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5641 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5701 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5761 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5821 aattttaaca aaattcaggg cgcaagggct gctaaaggaa gcggaacacg tagaaagcca
     5881 gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg
     5941 aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag
     6001 actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta
     6061 aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc
     6121 gcaggggatc aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     6181 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     6241 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     6301 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gtagggagga
     6361 ttttattatg tctactactt tggatgatac tgcttataga tacagaactt ctgttccagg
     6421 tgatgctgaa gctattgaag ctttggatgg ttctttcact accgatactg tttttagagt
     6481 tactgctact ggtgatggtt tcactttgag agaagttcca gttgatccac cattgactaa
     6541 ggttttccca gatgatgaat ccgatgatga atccgatgct ggtgaagatg gtgatccaga
     6601 ttctagaact ttcgttgctt atggtgatga tggtgatttg gctggtttcg ttgttgtttc
     6661 ttattctggt tggaacagaa gattgactgt tgaagatatt gaagttgctc cagaacatag
     6721 aggtcatggt gttggtagag ctttgatggg tttggctact gaattcgcca gagaaagagg
     6781 tgctggtcat ttgtggttgg aagttaccaa tgttaatgct ccagctattc atgcttatag
     6841 aagaatgggt ttcactttgt gtggtttgga tactgcttta tacgatggta ctgcttccga
     6901 tggtgaacaa gctttgtata tgtctatgcc atgtccataa tgcatgcccg acggcgagga
     6961 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     7021 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     7081 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     7141 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     7201 cttctgaatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     7261 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     7321 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     7381 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     7441 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     7501 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     7561 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     7621 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     7681 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     7741 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     7801 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     7861 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     7921 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     7981 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     8041 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     8101 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     8161 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     8221 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     8281 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     8341 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     8401 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     8461 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     8521 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     8581 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     8641 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     8701 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     8761 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     8821 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     8881 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     8941 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     9001 gagtcagtga gcgaggaagc ggaag
//
