LOCUS       Exported                8168 bp ds-DNA     circular SYN 05-JUL-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 2, 2019 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8168
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          6108..7444
                     /organism="Francisella tularensis subsp. holarctica"
                     /sub_species="holarctica"
                     /strain="LVS"
                     /mol_type="genomic DNA"
                     /note="/dnas_title=pKL115 pEX_PriM_mpk1B"
                     /db_xref="taxon:119857"
     primer_bind     4..26
                     /label=P025 pEX18KanSeq1F
                     /note="P025 pEX18KanSeq1F"
     primer_bind     474..493
                     /label=P037 pEX18KanSeq9F
                     /note="P037 pEX18KanSeq9F"
     primer_bind     733..755
                     /label=P026 pEX18KanSeq2F
                     /note="P026 pEX18KanSeq2F"
     promoter        866..1103
                     /note="groES promotoer"
     CDS             1104..1919
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1583..1607)
                     /label=P027 pEX18KanSeq3F
                     /note="P027 pEX18KanSeq3F"
     primer_bind     2117..2132
                     /label=P028 pEX18KanSeq4F (not entire 5' end)
                     /note="P028 pEX18KanSeq4F (not entire 5' end)"
     promoter        complement(2296..2400)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(2704..4125)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     primer_bind     2968..2994
                     /label=P029 pEX18KanSeq5F
                     /note="P029 pEX18KanSeq5F"
     primer_bind     3649..3675
                     /label=P030 pEX18KanSeq6F
                     /note="P030 pEX18KanSeq6F"
     promoter        complement(4126..4571)
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     primer_bind     4390..4416
                     /label=P031 pEX18KanSeq7F
                     /note="P031 pEX18KanSeq7F"
     oriT            4945..5054
                     /note="incP origin of transfer"
     primer_bind     5113..5137
                     /label=P033 pEX18KanSeq8F
                     /note="P033 pEX18KanSeq8F"
     terminator      5497..5524
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      5616..5702
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     6013..6036
                     /label=P023 pEX18SeqF2
                     /note="P023 pEX18SeqF2"
     primer_bind     6037..6056
                     /label=P021 pEX18SeqF1
                     /note="P021 pEX18SeqF1"
     primer_bind     6052..6068
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6102..6137
                     /label=P818 PriM_mpk_F2
     CDS             6108..7439
                     /codon_start=1
                     /locus_tag="FTL_0702"
                     /product="hypothetical protein"
                     /label=FTL_0702
                     /note="/dnas_title=hypothetical protein"
                     /db_xref="GI:89256091"
                     /db_xref="GeneID:3951071"
                     /inference="ab initio prediction"
                     /protein_id="YP_513453.1"
                     /translation="IVNESVTKYSNNVETDADTNTNSPIYAFKSIAADASNIQANGEKL
                     ARGFVLTNNGAVKIPNSIKPKNMYMKDQATAAQGLEKYRAERDEINNNIKKLESQKKLG
                     WRIKVVAEQAKLKSINTKIDILKGIENGDKAYAEEKIAQFNIVKNITVNGPKAYFNDIA
                     KPVLSHLNAAWDSATSLNYVDYRSNIDMFAGAAVVLANGQYNINSKDAATAIEFNDITN
                     YLTIQTIKSLQGSDTTKAVSLAADANTLTYTVTTIGDLSSVQKKIASPRTALSICEASL
                     ISIRTNTKVTTARNIINRLSNKKAVKPILRHLLNQTSIDDIILYDLDKLGDNWALKAAT
                     NAIRSTIGSDSILYVKGHTLMLISTSSMVDAFIKAIAENEIYKQVSDADRVLFGKNACN
                     FTAAKNSNNPIVKAMIAASKQIAGQLPKGQVIDTVFEEKVYLAL"
     gene            6108..7439
                     /locus_tag="FTL_0702"
                     /label=FTL_0702
                     /db_xref="GeneID:3951071"
     primer_bind     6343..6367
                     /label=P367 FTL0702seqF1
                     /note="P367 FTL0702seqF1"
     misc_feature    6440..6466
                     /label=nonstandard type: PCR_primer
                     /note="P429 FTL0702_VSVG_2R"
     misc_feature    6628..6651
                     /label=nonstandard type: PCR_primer
                     /note="P697 Ck_PriM_mpk1F"
     misc_feature    6628..6647
                     /label=nonstandard type: PCR_primer
                     /note="P435 RT_FTL0702-1F"
     misc_feature    complement(6663..6713)
                     /label=nonstandard type: PCR_primer
                     /note="P692 PriM_mpk_R1"
     misc_difference 6685..6840
                     /label=Altered region!
                     /note="Altered region!"
     misc_feature    6690..6742
                     /label=nonstandard type: PCR_primer
                     /note="P693 PriM_mpk_F2"
     misc_feature    complement(6726..6747)
                     /label=nonstandard type: PCR_primer
                     /note="P436 RT_FTL0702-1R"
     primer_bind     complement(6811..6847)
                     /label=P695 PriM_mpk_R3
     primer_bind     6831..6866
                     /label=P696 PriM_mpk_F4
     misc_feature    6836..6871
                     /label=nonstandard type: PCR_primer
                     /note="P696 PriM_mpk_F4"
     misc_feature    6843..6866
                     /label=nonstandard type: PCR_primer
                     /note="P439 RT_FTL0702-3F"
     misc_feature    complement(6911..6930)
                     /label=nonstandard type: PCR_primer
                     /note="P440 RT_FTL0702-3R"
     misc_feature    6951..6977
                     /label=nonstandard type: PCR_primer
                     /note="P444 pKL75_F"
     misc_feature    complement(7104..7126)
                     /label=nonstandard type: PCR_primer
                     /note="P698 Ck_PriM_mpk1R"
     misc_feature    complement(7114..7138)
                     /label=nonstandard type: PCR_primer
                     /note="P411 FTL0702sp_2R"
     primer_bind     complement(7183..7211)
                     /label=P368 FTL0702seqR1
                     /note="P368 FTL0702seqR1"
     misc_feature    7261..7283
                     /label=nonstandard type: PCR_primer
                     /note="P437 RT_FTL0702-2F"
     misc_feature    complement(7366..7387)
                     /label=nonstandard type: PCR_primer
                     /note="P438 RT_FTL0702-2R"
     primer_bind     complement(7413..7445)
                     /label=P819 PriM_mpk_R2
     gene            complement(7440..7444)
                     /locus_tag="FTL_0701"
                     /label=FTL_0701
                     /db_xref="GeneID:3951070"
     primer_bind     complement(7466..7482)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7477..7502)
                     /label=P024 pEX18SeqR2
                     /note="P024 pEX18SeqR2"
     protein_bind    7490..7506
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(7499..7518)
                     /label=P022 pEX18SeqR1
                     /note="P022 pEX18SeqR1"
     promoter        complement(7514..7544)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7559..7580
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(join(7868..8168,1..288))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
       61 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
      121 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
      181 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
      241 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
      301 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
      361 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
      421 tcaatctaaa gtatatatga gtaaacttgg tctgacaatc gatcaagggt tggtttgcgc
      481 attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc
      541 gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac
      601 gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg
      661 ctcgccgagg cggcataaat cgccgtgacg atcagcggtc caatgatcga agttaggctg
      721 gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac
      781 agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg
      841 aaggccatcc agcctcgcgt cgatctatac ccttcaagct ttgaaaaata aacttaatta
      901 ttatatatgt tatttagcta gtttttttaa ttaaagttaa aatcgagagc ttgtttgaca
      961 aaaaaacaaa aaaatttctt gaaaattttt tttttgactc aatatctaga cttgcaagag
     1021 cttggaactt tgagattgtt ctaagatgca tacaaattca aaatgcttaa acaaaaataa
     1081 tttaacaaag gagtaagatt gttatgagcc atattcaacg ggaaacgtct tgctcgaggc
     1141 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
     1201 tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt
     1261 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     1321 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     1381 atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat
     1441 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     1501 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc
     1561 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct
     1621 ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag
     1681 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     1741 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     1801 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     1861 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat
     1921 cagaattggt taattggttg taacactggc agagcattac gctgacttga cgggacggcg
     1981 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
     2041 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
     2101 caaagctctc atcaacgata tcccgcaaga ggcccggcag taccggcata accaagccta
     2161 tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca
     2221 tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagctaat
     2281 cggttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     2341 tctcatgagc ggatacatat ttgaatgtat ttagagaaat aaacaaatag gggttccgcg
     2401 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     2461 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     2521 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     2581 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     2641 ctatgcggca tcagagcaga ttgtactgag agtgcaccat aatcggcatt ttcttttgcg
     2701 tttttatttg ttaactgtta attgtccttg ttcaaggatg ctgtctttga caacagatgt
     2761 tttcttgcct ttgatgttca gcaggaagct aggcgcaaac gttgattgtt tgtctgcgta
     2821 gaatcctctg tttgtcatat agcttgtaat cacgacattg tttcctttcg cttgaggtac
     2881 agcgaagtgt gagtaagtaa aggttacatc gttaggatca agatccattt ttaacacaag
     2941 gccagttttg ttcagcggct tgtatgggcc agttaaagaa ttagaaacat aaccaagcat
     3001 gtaaatatcg ttagacgtaa tgccgtcaat cgtcattttt gatccgcggg agtcagtgaa
     3061 cagataccat ttgccgttca ttttaaagac gttcgcgcgt tcaatttcat ctgttactgt
     3121 gttagatgca atcagcggtt tcatcacttt tttcagtgtg taatcatcgt ttagctcaat
     3181 cataccgaga gcgccgtttg ctaactcagc cgtgcgtttt ttatcgcttt gcagaagttt
     3241 ttgactttct tgacggaaga atgatgtgct tttgccatag tatgctttgt taaataaaga
     3301 ttcttcgcct tggtagccat cttcagttcc agtgtttgct tcaaatacta agtatttgtg
     3361 gcctttatct tctacgtagt gaggatctct cagcgtatgg ttgtcgcctg agctgtagtt
     3421 gccttcatcg atgaactgct gtacattttg atacgttttt ccgtcaccgt caaagattga
     3481 tttataatcc tctacaccgt tgatgttcaa agagctgtct gatgctgata cgttaacttg
     3541 tgcagttgtc agtgtttgtt tgccgtaatg tttaccggag aaatcagtgt agaataaacg
     3601 gatttttccg tcagatgtaa atgtggctga acctgaccat tcttgtgttt ggtcttttag
     3661 gatagaatca tttgcatcga atttgtcgct gtctttaaag acgcggccag cgtttttcca
     3721 gctgtcaata gaagtttcgc cgactttttg atagaacatg taaatcgatg tgtcatccgc
     3781 atttttagga tctccggcta atgcaaagac gatgtggtag ccgtgatagt ttgcgacagt
     3841 gccgtcagcg ttttgtaatg gccagctgtc ccaaacgtcc aggccttttg cagaagagat
     3901 atttttaatt gtggacgaat cgaactcagg aacttgatat ttttcatttt tttgctgttc
     3961 agggatttgc agcatatcat ggcgtgtaat atgggaaatg ccgtatgttt ccttatatgg
     4021 cttttggttc gtttctttcg caaacgcttg agttgcgcct cctgccagca gtgcggtagt
     4081 aaaggttaat actgttgctt gttttgcaaa ctttttgatg ttcatcgttc atgtctcctt
     4141 ttttatgtac tgtgttagcg gtctgcttct tccagccctc ctgtttgaag atggcaagtt
     4201 agttacgcac aataaaaaaa gacctaaaat atgtaagggg tgacgccaaa gtatacactt
     4261 tgccctttac acattttagg tcttgcctgc tttatcagta acaaacccgc gcgatttact
     4321 tttcgacctc attctattag actctcgttt ggattgcaac tggtctattt tcctcttttg
     4381 tttgatagaa aatcataaaa ggatttgcag actacgggcc taaagaacta aaaaatctat
     4441 ctgtttcttt tcattctctg tattttttat agtttctgtt gcatgggcat aaagttgcct
     4501 ttttaatcac aattcagaaa atatcataat atctcatttc actaaataat agtgaacggc
     4561 aggtatatgt gatgggttaa aaaggatcga tcctctagct agagtcgatc ttcgccagca
     4621 gggcgaggat cgtggcatca ccgaaccgcg ccgtgcgcgg gtcgtcggtg agccagagtt
     4681 tcagcaggcc gcccaggcgg cccaggtcgc cattgatgcg ggccagctcg cggacgtgct
     4741 catagtccac gacgcccgtg attttgtagc cctggccgac ggccagcagg taggccgaca
     4801 ggctcatgcc ggctgccgcc gccttttcct caatcgctct tcgttcgtct ggaaggcagt
     4861 acaccttgat aggtgggctg cccttcctgg ttggcttggt ttcatcagcc atccgcttgc
     4921 cctcatctgt tacgccggcg gtagccggcc agcctcgcag agcaggattc ccgttgagca
     4981 ccgccaggtg cgaataaggg acagtgaaga aggaacaccc gctcgcgggt gggcctactt
     5041 cacctatcct gcccggctga cgccgttgga tacaccaagg aaagtctaca cgaacccttt
     5101 ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatc gctataatga
     5161 ccccgaagca gggttatgca gcggaaaagc gctgcttccc tgctgttttg tggaatatct
     5221 accgactgga aacaggcaaa tgcaggaaat tactgaactg aggggacagg cgagagacga
     5281 tgccaaagag ctacaccgac gagctggccg agtgggttga atcccgcgcg gccaagaagc
     5341 gccggcgtga tgaggctgcg gttgcgttcc tggcggtgag ggcggatgtc gatatgcggt
     5401 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgcata tttgaatgta
     5461 tttagaaaaa taaacaaaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc
     5521 ttctgcttaa tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc
     5581 cgttgcttcg caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc
     5641 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
     5701 tgatgcctgg cagttcccta ctctcgcatg gggagacccc acactaccat cggcgctacg
     5761 gcgtttcact tctgagttcg gcatggggtc aggtgggacc accgcgctac tgccgccagg
     5821 caaattctgt tttatcagac cgcttctgcg ttctgattta atctgtatca ggctgaaaat
     5881 cttctctcat ccgccaaaac agccaagctc gccattcgcc attcaggctg cgcaactgtt
     5941 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     6001 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     6061 cggccagtgc caagcttgca tgcctgcagg tcgactctag aggatccaat tgtaaatgaa
     6121 tctgttacta aatattctaa taatgttgag acagatgctg atactaatac aaattcacca
     6181 atttatgcat tcaaaagcat agcagctgat gcaagtaata ttcaagcaaa tggagaaaaa
     6241 ttagctagag gttttgtctt aactaacaat ggtgctgtta agataccgaa ctctatcaag
     6301 ccaaaaaata tgtatatgaa agatcaagca actgctgctc aaggtcttga gaaatacaga
     6361 gctgaaagag atgagattaa taacaacatc aaaaaacttg aaagtcaaaa gaaattaggc
     6421 tggagaatta aagtagttgc tgaacaagct aaactaaaaa gtattaacac taaaatagat
     6481 atcctaaaag gtatcgaaaa tggtgataaa gcttatgctg aagagaaaat cgctcagttt
     6541 aatatcgtaa aaaatataac tgtaaatggt cctaaggcgt attttaatga tattgctaag
     6601 ccagtattat cacatttaaa tgcagcatgg gattctgcta ctagtcttaa ctatgtagac
     6661 tacagaagca acattgatat gttcgcaggt gcagcagtag ttcttgcaaa tggtcaatat
     6721 aacatcaata gcaaagatgc cgctactgcc attgaattta acgatatcac taactacttg
     6781 actattcaga ctattaagtc tcttcaaggt agtgatacaa ctaaagcagt ttcactagca
     6841 gctgatgcta acacacttac atatacagta actactatag gcgacttaag tagtgttcag
     6901 aaaaaaatag cttcgccaag aacggctcta tcaatttgtg aagctagctt aatcagtatc
     6961 agaactaata ccaaagttac tacagctaga aatattatta acagactatc aaataaaaaa
     7021 gctgtaaaac ctatactaag acacttacta aatcaaacta gtattgatga tattatactt
     7081 tacgacctag ataaattagg cgacaactgg gcacttaaag cagctacaaa tgcaattaga
     7141 tcaacaatag gttctgatag tatactatac gttaagggtc atactttaat gttaatatct
     7201 acaagtagta tggttgatgc ctttatcaag gctattgctg agaatgaaat ttacaagcaa
     7261 gtaagtgatg ctgatagagt tctatttggt aaaaatgcat gtaattttac agcagctaaa
     7321 aatagtaaca atcctatagt taaagctatg attgcagctt ctaaacagat agccggacaa
     7381 cttcctaaag gtcaagttat cgacaccgtt ttcgaagaga aagtatattt ggctttacag
     7441 gtaccgagct cgaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc
     7501 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     7561 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7621 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7681 ggcgctcttc cgcttcctcg ctcactgacc cgctgcgctc ggtcgttcgg ctgcggcgag
     7741 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7801 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7861 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7921 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7981 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8041 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8101 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8161 ccggtaac
//
