LOCUS       Exported                6962 bp ds-DNA     circular SYN 17-JAN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 17, 2019 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=pF2"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        245..315
                     /note="gro no UP"
     primer_bind     273..295
                     /label=AROL2
     primer_bind     complement(467..489)
                     /label=AROL3
     gene            632..1507
                     /label=ORF3
                     /note="ORF3"
     gene            complement(1730..2149)
                     /label=ORF2
                     /note="ORF2"
     gene            complement(2155..3171)
                     /label=RepA
                     /note="RepA"
     CDS             4350..5144
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5162..6022
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6193..6781
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccttgaa aatttttttt ttgactcaat atctagactt gcaagagctt ggaactttga
      301 gattgttcta agatggaatt cccgggcggc cgctagctcg aggatccact agctcgtttc
      361 aaattaccga tgatatcgga ccgttccaac ttaccgacca gttcggcagg tatgtatttg
      421 cgtgcattcc tatccaaaaa aacatcaagc caaaagcttg aaaaaactta caacacagct
      481 caacagagct agattgtaaa accctgcttt gttaagcaga acgcaaaaat tgaatgactt
      541 atagtcatat cgcttcgacc ctcgtagatt agtagccttg agctattaac tggttgaaac
      601 acttaccaaa taaagattaa aagcgataaa aatgaaagat aaagcagcta aaaacagaga
      661 ttttagaaag actattttat cagtgttaca acgcaataaa gatggctctt ttgctacgca
      721 agcaaataga aagtctattc tgttgcaggc aactaaagac cttaaaaagg tagggtttag
      781 caaggttaca gccgaaaact tcggtaataa gcattgctat gcacttagag accattggag
      841 agcccaagga ttagctacag caacgataaa aaatcgttta gcttgtctaa ggtggttagg
      901 cgagaaaatg ggcaaagaac tacccgataa tcgaaaatta gagattgaga acaggaagta
      961 tagcgataat tcaatcaata aagcccaaga aatcgatttt aaggcgattt ctgccttaac
     1021 tgataggcaa gccctagcaa tacaattaca gcgcgaattt gggcttcgta gagaagaaag
     1081 tttgaagttt cagcctagtt atgcaatcaa agagcataaa atcgagctta aaagctcttg
     1141 gacaaagggt ggaagaccac gagaaatccc aattttgaat gaaaaacaga gagaattgtt
     1201 agaaaaagta aaagaggtag caggtaaagg ctctctaatt gagagcgaaa agtcttataa
     1261 gcaagcaatg gaacatttca cgactcgctg tcaaagagca gggattaaga atgttcatgg
     1321 ctttagacat gcgtatgctc aagatagata taggcaatta acagggcgtg agtgtcctaa
     1381 aaatggtgga ttaacatcta agcagctaac acctgagcaa aagcaacaag actatgaagc
     1441 tagaatgact attagtgaag agttaggtca tggtagagaa gatgtaacag tcaactactt
     1501 aggcagataa aaagcaatat agctatagaa gaaaagaaag ctattttaca tagtagatcg
     1561 actcttctta gggattttat attttttgat aaatcatcta ttttgctagt taaatcatca
     1621 aatttatcat cttgttgttt gactaaatct aagaatctat tctctttttt aaaatcgttc
     1681 atgcaaaccg cctatagctt tcttcttttt ctgaaattat ttgtcttcac accataatta
     1741 aattcccatt tttataagta aagtctttta aaagcttgtc agtctcttct ctagaaatgt
     1801 accaaatttt acctatttta ggatactttt catgaagctc ttctattttt ccccagtcct
     1861 ttaatagtct acctttagag tctcgtaaat agttatcttt gtgacagggg cctcttttat
     1921 cttttttaat gtaactatat gttattccaa cgtcactatt actattatcc aaatcttttt
     1981 tagcatgcca gtaagaactt tcataactta actctatctt tcgacctctt tgatatacaa
     2041 caataaagct atagccagta gtaacaacct gttttacttt tgttaaatct attaacttct
     2101 tatttatttt tttatgtttt tttgaaaatt taaatatttc tatattcatt cctacacttc
     2161 ctcaaatcca aatggtagct tatgattctc ttctggtttc ttttctaatt tttttatatt
     2221 tgcaataaaa actctttttc tatctttgat ttttttattg tcccaattcc tccaagtatc
     2281 atcacaaacc ctttcaatat catgtaaatg atgatgtcta aatattgatc tgacataata
     2341 cagatctagg tctagttcat cacttaacac aacttctcta agtctttcag atgcttcgat
     2401 tggtatgtaa tcctctttat ttttagtatc taaaagcttt tgcttaaatt cttcttctgt
     2461 ctctgctacc ttactaactg taaacttgat atttgtaatc ttacgaccat gttttctgtg
     2521 atgatccttg tcatcatagg ttacaaaaat atccgataat tgattaatct cttctagtgc
     2581 tggtaatagg aacttatttt taaaatttga atatctgttg ctgtaacttt taggtaaatc
     2641 aaaatcatta atcatatcat cgacatacaa tacgcaatca actatattag cataccctgc
     2701 ttgttcgcct aatttgcttt tgagaagtaa gtataatctg cttgaatact tacttttaaa
     2761 tgaaaatagt aactgtcttt ctgctttagt aaagtactct tgtagttgta tcatgtgtgg
     2821 cattaatgac caatgaaact cgcaaattaa agcactgctt ttagggtctg cttcaatata
     2881 tgcaaaccag ttagctatct tcgtttgttc tttattcagc catactggct tagacattat
     2941 tgagtgcatt aattgcttca atctcactct gttatgctta acccctgtag ctttttcaag
     3001 atcagatagg cttatcttat acctgtgaaa ctctttatct tctcttttaa ccattgaggc
     3061 aactaagaat attaagtttt gttcttcttt tgtaaggcta tactttcctg caacaagagt
     3121 attagacata gctatttctt tgccagcatt tacattttta acttctttca tagaactaga
     3181 gtcattatct cgatatacaa attctataaa acttctatta gtaaaacaac tacttcataa
     3241 aaaaaagtag ttttaacgat acaaaaagta gttttaaatt caaaaagtga tacaaaaagt
     3301 agttttaaat tcaaaaagtg atacaaaaag tagttttaaa ttttttaaaa aagtgcttca
     3361 aagccttatg tagcaatact tacagaggat taaaaaaaaa tctgacaata tataaagaga
     3421 atatataaag agaatatctt aggggatttt aaaaaaatcc cacagactca aagacttttt
     3481 tgacttttta aatcctagaa actatacttt aagtacttat ttaagtacat ggatttagat
     3541 tatgcaaacc gttaattatt caacttttag aaatgaacta tctgattcaa tggatagagt
     3601 aacaaaaaat catagtccta tgattgtaac tagaggttca aaaaaagaag cagttgttat
     3661 gatgtcgtta gaggattctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     3721 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     3781 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     3841 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     3901 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     3961 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaattcag
     4021 ggcgcaaggg ctgctaaagg aagcggaaca cgtagaaagc cagtccgcag aaacggtgct
     4081 gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga
     4141 gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga
     4201 cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca
     4261 aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga tcaagatctg
     4321 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     4381 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     4441 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     4501 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     4561 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     4621 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atcccacctt gctcctgccg
     4681 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     4741 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     4801 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     4861 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     4921 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     4981 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     5041 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     5101 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaattgaa aaaggaagag
     5161 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5221 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5281 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5341 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5401 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5461 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5521 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5581 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5641 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5701 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5761 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5821 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5881 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5941 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     6001 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     6061 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     6121 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     6181 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     6241 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     6301 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     6361 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6421 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6481 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6541 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6601 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6661 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6721 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6781 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6841 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     6901 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     6961 ag
//
