LOCUS       Exported                7234 bp ds-DNA     circular SYN 17-JAN-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 17, 2019 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7234
                     /organism="synthetic DNA construct"
                     /label=/dnas_title=pF
                     /note="/dnas_title=pF"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        299..532
                     /note="GroE"
     primer_bind     435..457
                     /label=AROL2
     primer_bind     complement(739..761)
                     /label=AROL3
     gene            904..1779
                     /label=ORF3
                     /note="ORF3"
     gene            complement(2002..2421)
                     /label=ORF2
                     /note="ORF2"
     gene            complement(2427..3443)
                     /label=RepA
                     /note="RepA"
     CDS             4622..5416
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5434..6294
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6465..7053
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccttgta tggattagtc gagctaaaaa gctcatattt tttatattca aactatatcc
      301 cttcaagctt tgaaaaataa acttaattat tatatatgtt atttagctag tttttttaat
      361 taaagttaaa atcgagagct tgtttgacaa aaaaacaaaa aaatttcttg aaaatttttt
      421 ttttgactca atatctagac ttgcaagagc ttggaacttt gagattgttc taagatgcat
      481 acaaattcaa aatgcttaaa caaaaataat ttaacaaagg agtaagattg ttatgaacat
      541 tcgtccatta caagatagag tattagttcg tcgtgcaggt accatatgaa ttcccgggcg
      601 gccgctagct cgaggatcca ctagctcgtt tcaaattacc gatgatatcg gaccgttcca
      661 acttaccgac cagttcggca ggtatgtatt tgcgtgcatt cctatccaaa aaaacatcaa
      721 gccaaaagct tgaaaaaact tacaacacag ctcaacagag ctagattgta aaaccctgct
      781 ttgttaagca gaacgcaaaa attgaatgac ttatagtcat atcgcttcga ccctcgtaga
      841 ttagtagcct tgagctatta actggttgaa acacttacca aataaagatt aaaagcgata
      901 aaaatgaaag ataaagcagc taaaaacaga gattttagaa agactatttt atcagtgtta
      961 caacgcaata aagatggctc ttttgctacg caagcaaata gaaagtctat tctgttgcag
     1021 gcaactaaag accttaaaaa ggtagggttt agcaaggtta cagccgaaaa cttcggtaat
     1081 aagcattgct atgcacttag agaccattgg agagcccaag gattagctac agcaacgata
     1141 aaaaatcgtt tagcttgtct aaggtggtta ggcgagaaaa tgggcaaaga actacccgat
     1201 aatcgaaaat tagagattga gaacaggaag tatagcgata attcaatcaa taaagcccaa
     1261 gaaatcgatt ttaaggcgat ttctgcctta actgataggc aagccctagc aatacaatta
     1321 cagcgcgaat ttgggcttcg tagagaagaa agtttgaagt ttcagcctag ttatgcaatc
     1381 aaagagcata aaatcgagct taaaagctct tggacaaagg gtggaagacc acgagaaatc
     1441 ccaattttga atgaaaaaca gagagaattg ttagaaaaag taaaagaggt agcaggtaaa
     1501 ggctctctaa ttgagagcga aaagtcttat aagcaagcaa tggaacattt cacgactcgc
     1561 tgtcaaagag cagggattaa gaatgttcat ggctttagac atgcgtatgc tcaagataga
     1621 tataggcaat taacagggcg tgagtgtcct aaaaatggtg gattaacatc taagcagcta
     1681 acacctgagc aaaagcaaca agactatgaa gctagaatga ctattagtga agagttaggt
     1741 catggtagag aagatgtaac agtcaactac ttaggcagat aaaaagcaat atagctatag
     1801 aagaaaagaa agctatttta catagtagat cgactcttct tagggatttt atattttttg
     1861 ataaatcatc tattttgcta gttaaatcat caaatttatc atcttgttgt ttgactaaat
     1921 ctaagaatct attctctttt ttaaaatcgt tcatgcaaac cgcctatagc tttcttcttt
     1981 ttctgaaatt atttgtcttc acaccataat taaattccca tttttataag taaagtcttt
     2041 taaaagcttg tcagtctctt ctctagaaat gtaccaaatt ttacctattt taggatactt
     2101 ttcatgaagc tcttctattt ttccccagtc ctttaatagt ctacctttag agtctcgtaa
     2161 atagttatct ttgtgacagg ggcctctttt atctttttta atgtaactat atgttattcc
     2221 aacgtcacta ttactattat ccaaatcttt tttagcatgc cagtaagaac tttcataact
     2281 taactctatc tttcgacctc tttgatatac aacaataaag ctatagccag tagtaacaac
     2341 ctgttttact tttgttaaat ctattaactt cttatttatt tttttatgtt tttttgaaaa
     2401 tttaaatatt tctatattca ttcctacact tcctcaaatc caaatggtag cttatgattc
     2461 tcttctggtt tcttttctaa tttttttata tttgcaataa aaactctttt tctatctttg
     2521 atttttttat tgtcccaatt cctccaagta tcatcacaaa ccctttcaat atcatgtaaa
     2581 tgatgatgtc taaatattga tctgacataa tacagatcta ggtctagttc atcacttaac
     2641 acaacttctc taagtctttc agatgcttcg attggtatgt aatcctcttt atttttagta
     2701 tctaaaagct tttgcttaaa ttcttcttct gtctctgcta ccttactaac tgtaaacttg
     2761 atatttgtaa tcttacgacc atgttttctg tgatgatcct tgtcatcata ggttacaaaa
     2821 atatccgata attgattaat ctcttctagt gctggtaata ggaacttatt tttaaaattt
     2881 gaatatctgt tgctgtaact tttaggtaaa tcaaaatcat taatcatatc atcgacatac
     2941 aatacgcaat caactatatt agcataccct gcttgttcgc ctaatttgct tttgagaagt
     3001 aagtataatc tgcttgaata cttactttta aatgaaaata gtaactgtct ttctgcttta
     3061 gtaaagtact cttgtagttg tatcatgtgt ggcattaatg accaatgaaa ctcgcaaatt
     3121 aaagcactgc ttttagggtc tgcttcaata tatgcaaacc agttagctat cttcgtttgt
     3181 tctttattca gccatactgg cttagacatt attgagtgca ttaattgctt caatctcact
     3241 ctgttatgct taacccctgt agctttttca agatcagata ggcttatctt atacctgtga
     3301 aactctttat cttctctttt aaccattgag gcaactaaga atattaagtt ttgttcttct
     3361 tttgtaaggc tatactttcc tgcaacaaga gtattagaca tagctatttc tttgccagca
     3421 tttacatttt taacttcttt catagaacta gagtcattat ctcgatatac aaattctata
     3481 aaacttctat tagtaaaaca actacttcat aaaaaaaagt agttttaacg atacaaaaag
     3541 tagttttaaa ttcaaaaagt gatacaaaaa gtagttttaa attcaaaaag tgatacaaaa
     3601 agtagtttta aattttttaa aaaagtgctt caaagcctta tgtagcaata cttacagagg
     3661 attaaaaaaa aatctgacaa tatataaaga gaatatataa agagaatatc ttaggggatt
     3721 ttaaaaaaat cccacagact caaagacttt tttgactttt taaatcctag aaactatact
     3781 ttaagtactt atttaagtac atggatttag attatgcaaa ccgttaatta ttcaactttt
     3841 agaaatgaac tatctgattc aatggataga gtaacaaaaa atcatagtcc tatgattgta
     3901 actagaggtt caaaaaaaga agcagttgtt atgatgtcgt tagaggattc ttcccttcct
     3961 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4021 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     4081 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4141 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     4201 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     4261 aaaaatttaa cgcgaatttt aacaaaattc agggcgcaag ggctgctaaa ggaagcggaa
     4321 cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc
     4381 tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac
     4441 atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg
     4501 ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc
     4561 aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgag gatcgtttcg
     4621 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     4681 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     4741 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     4801 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     4861 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     4921 ggatctcctg tcatcccacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     4981 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     5041 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     5101 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     5161 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     5221 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5281 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5341 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     5401 tgacgagttc ttctgaattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5461 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5521 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5581 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5641 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5701 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5761 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5821 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5881 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5941 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     6001 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     6061 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     6121 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6181 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6241 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6301 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6361 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6421 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6481 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6541 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6601 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6661 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6721 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6781 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6841 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6901 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6961 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7021 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7081 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     7141 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     7201 gagcgcagcg agtcagtgag cgaggaagcg gaag
//
