	   SnapGene      iatatGAATTCatgtcaaaacatattcatattttaggtatctgtggtacttttatgggttctttagcagtattagctaaacaaaagggatataaagtaacaggttcggatcttaatgtctatccaccaatgagtacttatctcgagtctcaaggcatagagatattacaaggatttgattgtgatcaactagatacaaatcctgatgaaattattattggtaatatcatgaaaagaggtatgccaataatagaaaaaatcctcgcagaaaagttaaactatttctcaggtccagagtggctatatcaaaatattcttaaatataaaaaagtaattgcaatagctggtacacatggtaaaactacaaccactacaatgactatcaaaatactagagcaggctggcttaaatccgagttttttggttggtggtgttagtagtgattttggtgtttcatcacgttacacagattctgagtattttgttattgaagctgatgaatatgatacggctttttttgataagcgttcaaaattgattcattatgatccaagtatttttgtgattaacaatattgaatttgatcatgctgatattttcaaagacattgatgcaatattttggcaatttcaccaattacttagaaagatgccatcaacagcaaagattatctacaacgctaagaatgataatgtacaaaaaataatatcaatgggatgttggtcagagttggttaaagtaaattctgatttaggtatcagtataaccaagcacacactagattactcaaagtttgaattatgtgatattaatggtaatagtgttgaagtatcatggggcttaattggtgagcataatgctcttaatgctatgagtgcctatgctgtagcaaagcaactaaatatatctgatgaaatggtaaaagacgctttagaaagctttagaggagttaaaagacgtttagaagtattatctcatcaagataatgttactttatatgatgattttgctcatcatccaacctctattaagttaactttagaagcagtgcgtaacaaagctaaagatgcttatgtagtagctcttattgacccacgttcaaatacaatgcgtcaaggcgataataaagataatttaccgatgtcaattatcgaagctgatagagtattgttgtataatcatagtttattaaagtgggatgctaaagaagttctcaaaaatagtaataatgttgattttatagctgatgttgatgattttgttgattgtgttgataaattattaactaagcaccaagatagaaatatccagcttgtaatgatgagtaatggctcgtttgatggattaagagaaaagctagttaagcttttggagatGGATCCatat                                  	                            
         	                                                          	   	                                                                                         
               	         	                                                          	      
   
   	                                                    
             	                                    	                                                         
               	         	            
                                                                                                                                                                                                                        	                                                                                    A T  6  B X   U i  ;^   L	 " +   W`+ 7 d   Z  l (C/      BY
   $f( O 7~	    	D  >    _'q%8[ V   M"]f   1
< j   D_  Y w ? ) &  / M 2 h s03 ^  Ha, * # C  ': ,  X	 4	 M 4 8 e F    Ut s      [ 
S   @  Nbmv{  Q   TJp I )a!I D*R X60     j   S    ! c    Y   #A
  C   	&eZ X %1g B ]  G&92)	 L   		 ? Puz H S 5HQ   R   X  W%d@ \ F%8 yGP Q [  EVx$cF  P     
1BCDc~E    2<J  L d p ~ t(U^P",    3=P8d.K`   J?oxHiw      M ` 5  e} k	 4 ^)?-9\     ud#&
 
 q .r
  H`V_n(Q	   Q/ Y  @S^ag     W   ,4>9Ne#d  L   K;\I< E    2y% x=   )@Pgpj   6 {   .{E< % k   w   !`    D  a 
 N   ir6N  \Z Z  	  fox~ .  |	  n { lu	  @ 5 @ :W] *  V_*K 'k.  Ne'	    =6    0CL^!$7 v> (  . r/ 3  r s R Ma IW`o)  i        01K > G Rt4 ?O K Z ;Ab#b o   I'-GT]_h!+ _ 4 
  3  cm- X +?   D    5(:Of$; $ v   -z;z 	   YhqM -  { m	  jJV  <B]5=6  - L
  = F ~ n :3&F\>N 2    ,:y  zl  ,Ai5U  1 < k  0  *+QhT Rq f    SrG 9 7   g   | 2      /|THVu    F= s &  t  : l 8Z8     x  "a       h       } \p      E3 T       b  " A     $W  O  0}UwI }  Os7cnwOv  j| 	  ]G>    Rc [  C [ n z t U  K ' q    J J " u *ybl gp 	 m  9}E[+9 / ; 
S    1   Y7     o     ! |  #v
 b y mB   k      A        GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  40  4R  4R  4T  4  5*  6H  = =   @ Xa!I ng   > %^ Cq^  i ^ PX       38d 'i ^ _<S5G Ls8[_'}S g ^       ,  XH S 5HQ Jj   D_	  ]G>H (SC 5/HQ 	   3 X    +?]G>Rcm-cDMa !b U  J?oxHiw n :3&F\>N! H c  S 5DHQ |  h7zXZ  ִF !   t/	&] ˇf{3(1v%f M*	mj)p80*i	NY]QF7&!ǚ^!8?>H=}&rRBsC܊M_uɊfhxlbY4Sǂ|z t|']kV߫=fbFTM۴%d3H&@Q~xz 0UzA(9f(-+A]a_y["2m8~)H΂VӚ/f֔upzH0& n(Cs0rMrBVQM"\pn,IƪGLG@MPL׶a&®D2Pm+msl@WH]{C+* M~6BNڂy<ٲÑR>_$ŠחYP ,!b P3&>Pe"v'Y:W@DgYmr)ݷQE5%@A`rH7tpLJ#K9iZ !%SQn΍'115班<W֒55[=iLՀwMihy/?{cOmy`䎾ag3 &X]~Uh SE}7X%}ڛorp"2i;M}Lnm VΠHK[{E{TGtAxg}E{Bm%;_Eh=
q::ӀV;d    r&c8[k   <g    YZ   -<AlignableSequences trimStringency="Medium"/>  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  g<?xml version="1.0"?><Features nextValidID="3"><Feature recentID="0" name="UDP-N-acetylmuramate" directionality="1" translationMW="51286.84" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1384" maxFusedRunOn="1384"><Segment range="11-1374" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSKHIHILGICGTFMGSLAVLAKQKGYKVTGSDLNVYPPMSTYLESQGIEILQGFDCDQLDTNPDEIIIGNIMKRGMPIIEKILAEKLNYFSGPEWLYQNILKYKKVIAIAGTHGKTTTTTMTIKILEQAGLNPSFLVGGVSSDFGVSSRYTDSEYFVIEADEYDTAFFDKRSKLIHYDPSIFVINNIEFDHADIFKDIDAIFWQFHQLLRKMPSTAKIIYNAKNDNVQKIISMGCWSELVKVNSDLGISITKHTLDYSKFELCDINGNSVEVSWGLIGEHNALNAMSAYAVAKQLNISDEMVKDALESFRGVKRRLEVLSHQDNVTLYDDFAHHPTSIKLTLEAVRNKAKDAYVVALIDPRSNTMRQGDNKDNLPMSIIEADRVLLYNHSLLKWDAKEVLKNSNNVDFIADVDDFVDCVDKLLTKHQDRNIQLVMMSNGSFDGLREKLVKLLE"/></Q></Feature><Feature recentID="1" name="FTL_0508" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11-1374" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11-1374" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="KROL670 FTL_0508_EcoRI_F" sequence="atatGAATTCatgtcaaaacatattcatattttaggtatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2023-05-18T15:16:04Z"><BindingSite location="0-39" boundStrand="0" annealedBases="atatGAATTCatgtcaaaacatattcatattttaggtatc" meltingTemperature="57"><Component hybridizedRange="0-39" bases="atatGAATTCatgtcaaaacatattcatattttaggtatc"/></BindingSite><BindingSite location="747-756" boundStrand="0" annealedBases="tttaggtatc" meltingTemperature="20"><Component bases="atatGAATTCatgtcaaaacatattcatat"/><Component hybridizedRange="747-756" bases="tttaggtatc"/></BindingSite><BindingSite simplified="1" location="0-39" boundStrand="0" annealedBases="atatGAATTCatgtcaaaacatattcatattttaggtatc" meltingTemperature="57"><Component hybridizedRange="0-39" bases="atatGAATTCatgtcaaaacatattcatattttaggtatc"/></BindingSite><BindingSite simplified="1" location="747-756" boundStrand="0" annealedBases="tttaggtatc" meltingTemperature="20"><Component bases="atatGAATTCatgtcaaaacatattcatat"/><Component hybridizedRange="747-756" bases="tttaggtatc"/></BindingSite></Primer><Primer recentID="1" name="KROL671 FTL_0508_BamHI_F" sequence="atatGGATCCatctccaaaagcttaactagcttttct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2023-05-18T15:16:04Z"><BindingSite location="1347-1383" boundStrand="1" annealedBases="atatGGATCCatctccaaaagcttaactagcttttct" meltingTemperature="62"><Component hybridizedRange="1347-1383" bases="atatGGATCCatctccaaaagcttaactagcttttct"/></BindingSite><BindingSite simplified="1" location="1347-1383" boundStrand="1" annealedBases="atatGGATCCatctccaaaagcttaactagcttttct" meltingTemperature="62"><Component hybridizedRange="1347-1383" bases="atatGGATCCatctccaaaagcttaactagcttttct"/></BindingSite></Primer></Primers>
  c<Notes>
<UUID>09157617-21a3-4b56-9520-abc5315a3903</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="15:16:7">2023.5.18</Created>
<LastModified UTC="15:16:7">2023.5.18</LastModified>
<CreatedBy>kramseylab</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         6+ Cutters                                                                                                  d     CHO  d              E             Y? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
