LOCUS       pSD26                   5172 bp    DNA     circular     18-JUL-2016
FEATURES             Location/Qualifiers
     PCR_primer      2783..2810
                     /note="P052 groESpromoF"
     promoter        complement(2783..3020)
                     /note="groES promoter from LVS"
     CDS             3021..3833
                     /note="kanamycin resistance (aphA1 from Tn903)"
     PCR_primer      3176..3200
                     /note="P442 pEX_kanR_F"
     primer_bind     complement(3500..3524)
                     /note="P027 pEX18KanSeq3F"
     PCR_primer      complement(3448..3470)
                     /note="P443 pEX_kanR_R"
     primer_bind     3003..3037
                     /note="SD38 (do not have)"
     PCR_primer      complement(2787..2814)
                     /note="P457 S_pSD26_1"
     PCR_primer      3803..3827
                     /note="P458 S_pSD26_2"
     misc_feature    3844..4702
                     /note="pSD26-P458.ab1"
     PCR_primer      complement(1843..1868)
                     /note="P470 S_pSD26_3"
     misc_feature    1784..2758
                     /note="pSD26-P457.ab1"
     PCR_primer      4619..4642
                     /note="P471 S_pSD26_4"
     PCR_primer      complement(3898..3924)
                     /note="P472 S_pSD26_5"
     PCR_primer      2666..2688
                     /note="P473 S_pSD26_6"
     source          1..4702
                     /dnas_title="pSD26"
     misc_feature    3834..3844
                     /note="pSD-P472"
     misc_feature    2759..2782
                     /note="pSD-P473"
     misc_feature    4703..5172
                     /note="pSD-P471"
     misc_feature    872..1783
                     /note="pSD26-P470"
     PCR_primer      1708..1733
                     /note="P474 S_pSD26_7"
     PCR_primer      complement(961..983)
                     /note="P475 S_pSD26_8"
     PCR_primer      complement(4772..4797)
                     /note="P477 S_pSD26_10"
     PCR_primer      complement(2726..2745)
                     /note="T7 primer"
     PCR_primer      4055..4074
                     /note="T7 primer"
     repeat_region   2694..2702
                     /note="5' ITR"
     repeat_region   4098..4106
                     /note="ITR"
     repeat_region   2680..2745
                     /note="Repeated Region"
     repeat_region   4055..4120
                     /note="Repeat Region"
     misc_feature    1..871
                     /note="pSD26-P475"
     PCR_primer      complement(45..61)
                     /note="M13 Reverse Primer"
     PCR_primer      251..272
                     /note="P476 S_pSD26_9"
     PCR_primer      2783..2810
                     /note="P506 KanCass_F"
     PCR_primer      complement(3810..3836)
                     /note="P507 KanCass_R"
     PCR_primer      2783..2810
                     /note="P506 KanCass_F"
     PCR_primer      complement(3810..3836)
                     /note="P507 KanCass_R"
     gene            complement(1208..2068)
                     /note="beta-lactamase"
     misc_feature    2766..2771
                     /note="MluI"
     gene            join(4143..5172,1..17)
                     /note="mariner transposase himar1C9"
ORIGIN      
        1 GGAACTATGT TGAATAATAA GCATGCAAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT
       61 GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT
      121 AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC
      181 GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG
      241 AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG
      301 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA
      361 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC
      421 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC
      481 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG
      541 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC
      601 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT
      661 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG
      721 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC
      781 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT
      841 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TTACGGCTAC ACTAGAAGAA CAGTATTTGG
      901 TATCTGCGCT CTGCTGAAGC CAGTTACCTT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG
      961 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA
     1021 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA
     1081 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA
     1141 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT
     1201 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT
     1261 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT
     1321 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG
     1381 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT
     1441 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT
     1501 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG
     1561 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA
     1621 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT
     1681 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT
     1741 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC
     1801 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA
     1861 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT
     1921 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT
     1981 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA
     2041 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT
     2101 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA
     2161 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA
     2221 TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTC GCGCGTTTCG
     2281 GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT
     2341 AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC
     2401 GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT
     2461 GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCCAT TCGCCATTCA
     2521 GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCTGG
     2581 CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC
     2641 GACGTTGTAA AACGACGGCC AGTGAATTCG AGCTCGGTAC CCGATTCCGG ATAACAGGTT
     2701 GGCTGATAAG TCCCCGGTCT CTAGACCCTA TAGTGAGTCG TATTACTCGA GCTAGCCATG
     2761 GCGCCACGCG TCCCGGCCGC ACtataccct tcaagctttg aaaaataaac ttaattatta
     2821 tatatgttat ttagctagtt tttttaatta aagttaaaat cgagagcttg tttgacaaaa
     2881 aaacaaaaaa atttcttgaa aatttttttt ttgactcaat atctagactt gcaagagctt
     2941 ggaactttga gattgttcta agatgcatac aaattcaaaa tgcttaaaca aaaataattt
     3001 aacaaaggag taagATTGTT ATGAGCCATA TTCAACGGGA AACGTCTTGC TCGAGGCCGC
     3061 GATTAAATTC CAACATGGAT GCTGATTTAT ATGGGTATAA ATGGGCTCGC GATAATGTCG
     3121 GGCAATCAGG TGCGACAATC TATCGATTGT ATGGGAAGCC CGATGCGCCA GAGTTGTTTC
     3181 TGAAACATGG CAAAGGTAGC GTTGCCAATG ATGTTACAGA TGAGATGGTC AGACTAAACT
     3241 GGCTGACGGA ATTTATGCCT CTTCCGACCA TCAAGCATTT TATCCGTACT CCTGATGATG
     3301 CATGGTTACT CACCACTGCG ATCCCCGGAA AAACAGCATT CCAGGTATTA GAAGAATATC
     3361 CTGATTCAGG TGAAAATATT GTTGATGCGC TGGCAGTGTT CCTGCGCCGG TTGCATTCGA
     3421 TTCCTGTTTG TAATTGTCCT TTTAACAGCG ATCGCGTATT TCGTCTCGCT CAGGCGCAAT
     3481 CACGAATGAA TAACGGTTTG GTTGATGCGA GTGATTTTGA TGACGAGCGT AATGGCTGGC
     3541 CTGTTGAACA AGTCTGGAAA GAAATGCATA AACTTTTGCC ATTCTCACCG GATTCAGTCG
     3601 TCACTCATGG TGATTTCTCA CTTGATAACC TTATTTTTGA CGAGGGGAAA TTAATAGGTT
     3661 GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA GGATCTTGCC ATCCTATGGA
     3721 ACTGCCTCGG TGAGTTTTCT CCTTCATTAC AGAAACGGCT TTTTCAAAAA TATGGTATTG
     3781 ATAATCCTGA TATGAATAAA TTGCAGTTTC ATTTGATGCT CGATGAGTTT TTCTAATCAG
     3841 AATTGGTTAA TTGGTTGTAA CACTGGCAGA GCATTACGCT GACTTGACGG GACGGCGGCT
     3901 TTGTTGAATA AATCGAACTT TTGCTGAGTT GAAGGATCAG ATCACGCATC TTCCCGACAA
     3961 CGCAGACCGT TCCGTGGCAA AGCAAAAGTT CAAAATCACC AACTGGTCCA CCTACAACAA
     4021 AGCTCTCATC AACCGTGGCG GGGATCGGGA ATTCTAATAC GACTCACTAT AGGGTCTAGA
     4081 GACCGGGGAC TTATCAGCCA ACCTGTTATC CGGAATCGGG GATCCAAAGG AGTAAGATTG
     4141 TTATGGAAAA AAAGGAATTT CGTGTTTTGA TAAAATACTG TTTTCTGAAG GGAAAAAATA
     4201 CAGTGGAAGC AAAAACTTGG CTTGATAATG AGTTTCCGGA CTCTGCCCCA GGGAAATCAA
     4261 CAATAATTGA TTGGTATGCA AAATTCAAGC GTGGTGAAAT GAGCACGGAG GACGGTGAAC
     4321 GCAGTGGACG CCCGAAAGAG GTGGTTACCG ACGAAAACAT CAAAAAAATC CACAAAATGA
     4381 TTTTGAATGA CCGTAAAATG AAGTTGATCG AGATAGCAGA GGCCTTAAAG ATATCAAAGG
     4441 AACGTGTTGG TCATATCATT CATCAATATT TGGATATGCG GAAGCTCTGT GCGAAATGGG
     4501 TGCCGCGCGA GCTCACATTT GACCAAAAAC AACGACGTGT TGATGATTCT AAGCGGTGTT
     4561 TGCAGCTGTT AACTCGTAAT ACACCCGAGT TTTTCCGTCG ATATGTGACA ATGGATGAAA
     4621 CATGGCTCCA TCACTACACT CCTGAGTCCA ATCGACAGTC GGCTGAGTGG ACAGCGACCG
     4681 GTGAACCGTC TCCGAAGCGT GGAAAGACTC AAAAGTCCGC TGGCAAAGTA ATGGCCTCTG
     4741 TTTTTTGGGA TGCGCATGGA ATAATTTTTA TCGATTATCT TGAGAAGGGA AAAACCATCA
     4801 ACAGTGACTA TTATATGGCG TTATTGGAGC GTTTGAAGGT CGAAATCGCG GCAAAACGGC
     4861 CCCACATGAA GAAGAAAAAA GTGTTGTTCC ACCAAGACAA CGCACCGTGC CACAAGTCAT
     4921 TGAGAACGAT GGCAAAAATT CATGAATTGG GCTTCGAATT GCTTCCCCAC CCGCCGTATT
     4981 CTCCAGATCT GGCCCCCAGC GACTTTTTCT TGTTCTCAGA CCTCAAAAGG ATGCTCGCAG
     5041 GGAAAAAATT TGGCTGCAAT GAAGAGGTGA TCGCCGAAAC TGAGGCCTAT TTTGAGGCAA
     5101 AACCGAAGGA GTACTACCAA AATGGTATCA AAAAATTGGA AGGTCGTTAT AATCGTTGTA
     5161 TCGCTCTTGA AG
//

