LOCUS       Exported                4616 bp DNA     circular SYN 27-MAY-2022
DEFINITION  -.
ACCESSION   -
VERSION     .
KEYWORDS    -
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 29, 2022 from SnapGene 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2542..2749
                     /label=pSD26
                     /note="pSD26"
                     /note="/dnas_title=pKL97_Ft_InSeq_Tn10"
     CDS             complement(1..861)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2..26
                     /label=P497 Seq_pSam_Bt2_1
     misc_feature    593..613
                     /label=PCR_primer
                     /label=nonstandard type:  PCR_primer
                     /note="P498 Seq_pSam_Bt2_2"
     promoter        complement(862..966)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(896..902)
                     /note="P3"
     misc_feature    complement(1062..1067)
                     /label=MluI
                     /note="MluI"
     mobile_element  1078..2535
                     /mobile_element_type="transposon"
                     /label=transposon
                     /note="transposon"
     misc_feature    1078..1106
                     /label=MmeI IR_L
                     /label=nonstandard type:  frag
                     /note="MmeI IR_L"
     misc_feature    1084..1089
                     /label=MmeI site
                     /label=nonstandard type:  frag
                     /note="MmeI"
     primer_bind     1102..1127
                     /label=BioSamA
                     /note="BioSamA"
     misc_feature    complement(1107..1126)
                     /label=frag
                     /label=nonstandard type:  frag
                     /note="P7"
     primer_bind     complement(1127..1147)
                     /label=P573 Tn_Mar4
     primer_bind     complement(1128..1151)
                     /label=P571 Tn_Mar2
     misc_feature    1146..1182
                     /label=P508 KanCass_F
                     /note="P508 KanCass_F"
     promoter        1155..1392
                     /label=groES promoter
                     /note="groES promoter from LVS"
     misc_feature    1155..1182
                     /label=P052 groESpromoF
                     /note="P052 groESpromoF"
     misc_feature    complement(1159..1186)
                     /label=P457 S_pSD26_1
                     /note="P457 S_pSD26_1"
     primer_bind     complement(1160..1187)
                     /label=P572 Tn_Mar3
     primer_bind     complement(1225..1251)
                     /label=P570 Tn_Mar1
     primer_bind     1375..1409
                     /label=SD38 (do not have)
                     /note="SD38 (do not have)"
     CDS             1393..2208
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferaseaphA1 from Tn903
                     "
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    1548..1572
                     /label=PCR_primer
                     /label=nonstandard type:  PCR_primer
                     /note="P442 pEX_kanR_F"
     misc_feature    complement(1820..1842)
                     /label=PCR_primer
                     /label=nonstandard type:  PCR_primer
                     /note="P443 pEX_kanR_R"
     primer_bind     complement(1872..1896)
                     /label=P027 pEX18KanSeq3F
                     /note="P027 pEX18KanSeq3F"
     misc_feature    2175..2199
                     /label=P458 S_pSD26_2
                     /note="P458 S_pSD26_2"
     misc_feature    complement(2182..2223)
                     /label=P509 KanCass_R
                     /note="P509 KanCass_R"
     misc_feature    2229..2472
                     /label=frag
                     /label=nonstandard type:  frag
                     /note="3957 to 4200 of pFW11"
     misc_feature    2241..2265
                     /label=PCR_primer
                     /label=nonstandard type:  PCR_primer
                     /note="P501 Seq_pSam_Bt2_5"
     terminator      2256..2342
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2259..2302
                     /note="E. coli rrnB terminator T1"
     terminator      2434..2461
                     /note="E. coli rrnB terminator T2"
     primer_bind     2477..2486
                     /label=P573 Tn_Mar4
     primer_bind     2486..2511
                     /label=BioSamA
                     /note="BioSamA"
     misc_feature    2487..2506
                     /label=frag
                     /label=nonstandard type:  frag
                     /note="P7"
     misc_feature    2507..2535
                     /label=MmeI IR_R
                     /label=nonstandard type:  frag
                     /note="MmeI IR_R"
     misc_feature    2524..2529
                     /label=MmeI site
                     /label=nonstandard type:  frag
                     /note="MmeI"
     misc_feature    2524
                     /label=G->T
                     /label=nonstandard type:  frag
                     /note="G->T creates MmeI site"
     misc_feature    2536..2566
                     /label=PCR_primer
                     /label=nonstandard type:  PCR_primer
                     /note="P580 PpSD26_F1"
     -35_signal      2578..2583
     -10_signal      2599..2600
     -10_signal      2602..2607
     misc_feature    complement(2728..2764)
                     /label=PCR_primer
                     /label=nonstandard type:  PCR_primer
                     /note="P581 PpSD26_R1"
     RBS             2750..2755
                     /note="RBS"
     misc_feature    2759..2764
                     /label=NdeI
                     /note="NdeI"
     gene            2762..3808
                     /label=mariner transposase himar1C9
                     /note="mariner transposase himar1C9"
     misc_feature    3180..3204
                     /label=PCR_primer
                     /label=nonstandard type:  PCR_primer
                     /note="P503 Seq_pSam_Bt2_7"
     misc_feature    3731..3758
                     /label=PCR_primer
                     /label=nonstandard type:  PCR_primer
                     /note="P504 Seq_pSam_Bt2_8"
     misc_feature    complement(3837..4610)
                     /label=RP4-OriT-OriR6K
                     /note="RP4-OriT-OriR6K
                     "
     rep_origin      3841..4229
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     primer_bind     3841..3864
                     /label=KROL375  R6KG_ori_NspI_F
     primer_bind     complement(4205..4229)
                     /label=KROL376  R6KG_ori_NspI_R
     oriT            4344..4453
                     /note="incP origin of transfer"
     misc_feature    complement(4518..4542)
                     /label=PCR_primer
                     /label=nonstandard type:  PCR_primer
                     /note="P505 Seq_pSam_Bt2_9"
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     1021 attaacctat aaaaataggc gtatcacgag gccctttcgt cacgcgtcct cggtacctaa
     1081 caggttggat gataagtccc cggtcttcgt atgccgtctt ctgcttggcg cgccctcgag
     1141 caattatcca attgtatacc cttcaagctt tgaaaaataa acttaattat tatatatgtt
     1201 atttagctag tttttttaat taaagttaaa atcgagagct tgtttgacaa aaaaacaaaa
     1261 aaatttcttg aaaatttttt ttttgactca atatctagac ttgcaagagc ttggaacttt
     1321 gagattgttc taagatgcat acaaattcaa aatgcttaaa caaaaataat ttaacaaagg
     1381 agtaagattg ttatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat
     1441 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     1501 ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     1561 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     1621 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     1681 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     1741 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     1801 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     1861 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     1921 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     1981 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     2041 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     2101 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     2161 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaacc atgggctagc
     2221 gatctagaat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga
     2281 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
     2341 atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac
     2401 gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt
     2461 ttgcgtttct acctgcaggg cgcgccaagc agaagacggc atacgaagac cggggactta
     2521 tcatccaacc tgttaggatc ctaattggtt gtaacactgg cagagcatta cgctgacttg
     2581 acgggacggc ggctttgttg aataaatcga acttttgctg agttgaagga tcagatcacg
     2641 catcttcccg acaacgcaga ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg
     2701 tccacctaca acaaagctct catcaaccgt ggcggggatc gggaattcta ggaggtaaca
     2761 tatggaaaaa aaggaatttc gtgttttgat aaaatactgt tttctgaagg gaaaaaatac
     2821 agtggaagca aaaacttggc ttgataatga gtttccggac tctgccccag ggaaatcaac
     2881 aataattgat tggtatgcaa aattcaagcg tggtgaaatg agcacggagg acggtgaacg
     2941 cagtggacgc ccgaaagagg tggttaccga cgaaaacatc aaaaaaatcc acaaaatgat
     3001 tttgaatgac cgtaaaatga agttgatcga gatagcagag gccttaaaga tatcaaagga
     3061 acgtgttggt catatcattc atcaatattt ggatatgcgg aagctctgtg cgaaatgggt
     3121 gccgcgcgag ctcacatttg accaaaaaca acgacgtgtt gatgattcta agcggtgttt
     3181 gcagctgtta actcgtaata cacccgagtt tttccgtcga tatgtgacaa tggatgaaac
     3241 atggctccat cactacactc ctgagtccaa tcgacagtcg gctgagtgga cagcgaccgg
     3301 tgaaccgtct ccgaagcgtg gaaagactca aaagtccgct ggcaaagtaa tggcctctgt
     3361 tttttgggat gcgcatggaa taatttttat cgattatctt gagaagggaa aaaccatcaa
     3421 cagtgactat tatatggcgt tattggagcg tttgaaggtc gaaatcgcgg caaaacggcc
     3481 ccacatgaag aagaaaaaag tgttgttcca ccaagacaac gcaccgtgcc acaagtcatt
     3541 gagaacgatg gcaaaaattc atgaattggg cttcgaattg cttccccacc cgccgtattc
     3601 tccagatctg gcccccagcg actttttctt gttctcagac ctcaaaagga tgctcgcagg
     3661 gaaaaaattt ggctgcaatg aagaggtgat cgccgaaact gaggcctatt ttgaggcaaa
     3721 accgaaggag tactaccaaa atggtatcaa aaaattggaa ggtcgttata atcgttgtat
     3781 cgctcttgaa gggaactatg ttgaataagc ggccgccacc gcggtggagg ggaattccca
     3841 tgtcagccgt taagtgttcc tgtgtcactc aaaattgctt tgagaggctc taagggcttc
     3901 tcagtgcgtt acatccctgg cttgttgtcc acaaccgtta aaccttaaaa gctttaaaag
     3961 ccttatatat tctttttttt cttataaaac ttaaaacctt agaggctatt taagttgctg
     4021 atttatatta attttattgt tcaaacatga gagcttagta cgtgaaacat gagagcttag
     4081 tacgttagcc atgagagctt agtacgttag ccatgagggt ttagttcgtt aaacatgaga
     4141 gcttagtacg ttaaacatga gagcttagta cgtgaaacat gagagcttag tacgtactat
     4201 caacaggttg aactgctgat cttcagatcc tctacgccgg acgcatcgtg gccggatcaa
     4261 ttccgtgata ggtgggctgc ccttcctggt tggcttggtt tcatcagcca tccgcttgcc
     4321 ctcatctgtt acgccggcgg tagccggcca gcctcgcaga gcaggattcc cgttgagcac
     4381 cgccaggtgc gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg ggcctacttc
     4441 acctatcctg cccggctgac gccgttggat acaccaagga aagtctacac gaaccctttg
     4501 gcaaaatcct gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg ctataatgac
     4561 cccgaagcag ggttatgcag cggaaaacgg aattgatccc cctcgaattg acgcgt
//
