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      
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 b l9G		2


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>  
&4J`v 
  Qgv1	%}<R   o{t		V]   j Mb#
` #WA"h{ 	P		=
Q

+\>oB   zus0	U31 ef/zVop			P

;<=STBC . g(BWq		
?D / nC	
8 3 bfh]^r
r!q 8zK)X	
y)EKU qa}p

@,*	 -N$	
  	J
Y 0 M CQT oD	
9 ;I$aa    g~\Mb
NX {,ei#^s	

Ws%tU8 4 c5Y_	
"  K#Wg

W4r & p 9{*	H<72  *  )L	
zL Y_Olx{7n
`F  GY/	
*LV  l~A	
A	" 7    	
,s  b*,?[1		
-y G rHeq
e+ a  kF	r		1


d  InL	h		C5  n.LieO%		  J`kc		6
9

   QsR Z	[		K
BA # \ x8,Z
 MDt
k> N D


xnf
 p>%| 1 ,U?	V		
#^{$:P. K Rc!(vE  v&<	NPb  '0R%j
z
Gb  NJ,	&
$^  xC	{			"8 = 8UN2v			Od%;    P]'e		
c
|
.O   x  xYL      h	5; |13	t% (2Y_lz6t=		



(m	b*V9    fj$ET[&w  )u-`		w



Xhuw 1I+AWm 9 }ZU		
 5d6x`!
Z 5 n  A	=			l

#rc	  LG$Xh
 {Xu
 
5   

5Zs q !f	

&3h B :+j3		(
U
 '  		=8/ }  |	I		It{ *
 4Ma
{M  +  A~v	 Y :	
m Z/98,
.3aGl 	 i Pf	I_u  d@.n		<
O
AA a`my|]o
Tp P,HM``   +X"L
VMT7W X^z.	
G;6K F   kZ0	+    m_KhE	g		B	  P+s

 J u


$"z-   u/B	~	M#! 7] w     1	N-::  t0D		
a 8 m  4<		q*  z
t A  2				.gz	  3@

lu c-\		  +yL5S I ^Jgf	}	r
 ,\~ v  sM9 f H u IfL78  )  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                                                                                      	                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          	   
               	                     
                                 	                                                            	                                                   G   D         9   6   !            :                                                                                                                                                                                                             	                                                                                                                                                                                                                                                                                                                                                                                                                                                    	 
     	       
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text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;div&gt;aphA1 from Tn903&lt;/div&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature recentID="5" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="792" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="1-792" color="#ccffcc" type="standard" 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consecutiveTranslationNumbering="1"><Segment range="1084-1089" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  frag"/></Q><Q name="note"><V text="MmeI"/></Q></Feature><Feature recentID="17" name="BioSamA" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1102-1155" color="#a020f0" type="standard"/><Q name="note"><V text="BioSamA"/></Q></Feature><Feature recentID="18" name="frag" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1141-1154" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  frag"/></Q><Q name="note"><V text="P7"/></Q></Feature><Feature recentID="19" name="groES promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1183-1420" color="#ffffff" type="standard"/><Q name="note"><V text="groES promoter from LVS"/></Q></Feature><Feature recentID="20" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1576-1600" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P442 pEX_kanR_F"/></Q></Feature><Feature recentID="21" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1900-1924" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="22" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1848-1870" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P443 pEX_kanR_R"/></Q></Feature><Feature recentID="23" name="frag" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2257-2500" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  frag"/></Q><Q name="note"><V text="3957 to 4200 of pFW11"/></Q></Feature><Feature recentID="24" name="terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2287-2330" color="#ffffff" type="standard"/><Q name="note"><V text="E. coli rrnB terminator T1"/></Q></Feature><Feature recentID="25" name="terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2462-2489" color="#ffffff" type="standard"/><Q name="note"><V text="E. coli rrnB terminator T2"/></Q></Feature><Feature recentID="26" name="BioSamA" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2514-2539" color="#a020f0" type="standard"/><Q name="note"><V text="BioSamA"/></Q></Feature><Feature recentID="27" name="frag" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2515-2534" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  frag"/></Q><Q name="note"><V text="P7"/></Q></Feature><Feature recentID="28" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2269-2293" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P501 Seq_pSam_Bt2_5"/></Q></Feature><Feature recentID="29" name="MmeI IR_R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2535-2563" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  frag"/></Q><Q name="note"><V text="MmeI IR_R"/></Q></Feature><Feature recentID="30" name="G-&gt;T" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2552-2552" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  frag"/></Q><Q name="note"><V text="G-&gt;T creates MmeI site"/></Q></Feature><Feature recentID="31" name="MmeI site" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2552-2557" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  frag"/></Q><Q name="note"><V text="MmeI"/></Q></Feature><Feature recentID="32" name="-10 signal" type="-10_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2630-2635" color="#a6acb3" type="standard"/></Feature><Feature recentID="33" name="-35 signal" type="-35_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2606-2611" color="#a6acb3" type="standard"/></Feature><Feature recentID="34" name="-10 signal" type="-10_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2627-2628" color="#a6acb3" type="standard"/></Feature><Feature recentID="35" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2564-2594" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P580 PpSD26_F1"/></Q></Feature><Feature recentID="36" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2756-2792" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P581 PpSD26_R1"/></Q></Feature><Feature recentID="37" name="pSD26" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="2570-2777" color="#ffffff" type="standard"/><Q name="note"><V text="pSD26"/><V text="/dnas_title=pKL97_Ft_InSeq_Tn10"/></Q></Feature><Feature recentID="38" name="RBS" type="RBS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2778-2783" color="#a6acb3" type="standard"/><Q name="note"><V text="RBS"/></Q></Feature><Feature recentID="39" name="RP4-OriT-OriR6K" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3865-4638" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;RP4-OriT-OriR6K&lt;br/&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="P509 KanCass_R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="2210-2251" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;P509 KanCass_R&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="P508 KanCass_F" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1174-1210" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;P508 KanCass_F&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="42" name="SD38 (do not have)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1403-1437" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SD38 (do not have)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="43" name="P052 groESpromoF" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1183-1210" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;P052 groESpromoF&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="44" name="P457 S_pSD26_1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="1187-1214" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;P457 S_pSD26_1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="45" name="P458 S_pSD26_2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2203-2227" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;P458 S_pSD26_2&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="46" name="Promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2565-2639" color="#ffffff" type="standard"/></Feature><Feature recentID="48" name="MmeI site" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" visible="0"><Segment range="1113-1118" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  frag"/></Q><Q name="note"><V text="MmeI"/></Q></Feature><Feature recentID="49" name="BioSamA" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" visible="0"><Segment range="1131-1140" color="#a020f0" type="standard"/><Q name="note"><V text="BioSamA"/></Q></Feature><Feature recentID="50" name="frag" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1136-1140" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  frag"/></Q><Q name="note"><V text="P7"/></Q></Feature><Feature recentID="52" name="IR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1107-1135" color="#a6acb3" type="standard"/></Feature></Features>
  /e<?xml version="1.0"?><Primers nextValidID="11"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="P497 Seq_pSam_Bt2_1" sequence="TACCAATGCTTAATCAGTGAGGCAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1-25" boundStrand="0" annealedBases="TACCAATGCTTAATCAGTGAGGCAC" meltingTemperature="59"><Component hybridizedRange="1-25" bases="TACCAATGCTTAATCAGTGAGGCAC"/></BindingSite><BindingSite simplified="1" location="1-25" boundStrand="0" annealedBases="TACCAATGCTTAATCAGTGAGGCAC" meltingTemperature="59"><Component hybridizedRange="1-25" bases="TACCAATGCTTAATCAGTGAGGCAC"/></BindingSite></Primer><Primer recentID="1" name="P573 Tn_Mar4" sequence="ATAATTGCTCGAGGGCGCGCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1154-1174" boundStrand="1" annealedBases="ATAATTGCTCGAGGGCGCGCC" meltingTemperature="65"><Component hybridizedRange="1154-1174" bases="ATAATTGCTCGAGGGCGCGCC"/></BindingSite><BindingSite location="2504-2513" boundStrand="0" annealedBases="AGGGCGCGCC" meltingTemperature="54"><Component bases="ATAATTGCTCG"/><Component hybridizedRange="2504-2513" bases="AGGGCGCGCC"/></BindingSite><BindingSite simplified="1" location="1154-1174" boundStrand="1" annealedBases="ATAATTGCTCGAGGGCGCGCC" meltingTemperature="65"><Component hybridizedRange="1154-1174" bases="ATAATTGCTCGAGGGCGCGCC"/></BindingSite><BindingSite simplified="1" location="2504-2513" boundStrand="0" annealedBases="AGGGCGCGCC" meltingTemperature="54"><Component bases="ATAATTGCTCG"/><Component hybridizedRange="2504-2513" bases="AGGGCGCGCC"/></BindingSite></Primer><Primer recentID="2" name="P572 Tn_Mar3" sequence="attaagtttatttttcaaagcttgaagg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1187-1214" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="1187-1214" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite><BindingSite simplified="1" location="1187-1214" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="1187-1214" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite></Primer><Primer recentID="3" name="P571 Tn_Mar2" sequence="TTGGATAATTGCTCGAGGGCGCGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1155-1178" boundStrand="1" annealedBases="TTGGATAATTGCTCGAGGGCGCGC" meltingTemperature="66"><Component hybridizedRange="1155-1178" bases="TTGGATAATTGCTCGAGGGCGCGC"/></BindingSite><BindingSite simplified="1" location="1155-1178" boundStrand="1" annealedBases="TTGGATAATTGCTCGAGGGCGCGC" meltingTemperature="66"><Component hybridizedRange="1155-1178" bases="TTGGATAATTGCTCGAGGGCGCGC"/></BindingSite></Primer><Primer recentID="4" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1252-1278" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="1252-1278" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite location="3792-3801" boundStrand="1" annealedBases="cgatttaac" meltingTemperature="11"><Component bases="tttgtcaaacaagctct"/><Component hybridizedRange="3797-3801" bases="cgatt"/><Component bases="t"/><Component hybridizedRange="3792-3795" bases="taac"/></BindingSite><BindingSite simplified="1" location="1252-1278" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="1252-1278" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="3792-3801" boundStrand="1" annealedBases="cgatttaac" meltingTemperature="11"><Component bases="tttgtcaaacaagctct"/><Component hybridizedRange="3797-3801" bases="cgatt"/><Component bases="t"/><Component hybridizedRange="3792-3795" bases="taac"/></BindingSite></Primer><Primer recentID="5" name="KROL375  R6KG_ori_NspI_F" sequence="atatacatgtTGTCAGCCGTTAAGTGTTCCTGTG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-11-30T19:28:46Z"><BindingSite location="3868-3891" boundStrand="0" annealedBases="TGTCAGCCGTTAAGTGTTCCTGTG" meltingTemperature="62"><Component bases="atatacatgt"/><Component hybridizedRange="3868-3891" bases="TGTCAGCCGTTAAGTGTTCCTGTG"/></BindingSite><BindingSite simplified="1" location="3868-3891" boundStrand="0" annealedBases="TGTCAGCCGTTAAGTGTTCCTGTG" meltingTemperature="62"><Component bases="atatacatgt"/><Component hybridizedRange="3868-3891" bases="TGTCAGCCGTTAAGTGTTCCTGTG"/></BindingSite></Primer><Primer recentID="6" name="KROL376  R6KG_ori_NspI_R" sequence="atatacatgtGATCTGAAGATCAGCAGTTCAACCT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-11-30T19:30:47Z"><BindingSite location="4232-4256" boundStrand="1" annealedBases="GATCTGAAGATCAGCAGTTCAACCT" meltingTemperature="59"><Component bases="atatacatgt"/><Component hybridizedRange="4232-4256" bases="GATCTGAAGATCAGCAGTTCAACCT"/></BindingSite><BindingSite simplified="1" location="4232-4256" boundStrand="1" annealedBases="GATCTGAAGATCAGCAGTTCAACCT" meltingTemperature="59"><Component bases="atatacatgt"/><Component hybridizedRange="4232-4256" bases="GATCTGAAGATCAGCAGTTCAACCT"/></BindingSite></Primer><Primer recentID="7" name="KROL517 Tn_lnSeqA_F" sequence="ATATGGTACCTAACAGGTTGGATGATAAG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2022-05-23T19:16:50Z"><BindingSite location="1071-1095" boundStrand="0" annealedBases="GGTACCTAACAGGTTGGATGATAAG" meltingTemperature="57"><Component bases="ATAT"/><Component hybridizedRange="1071-1095" bases="GGTACCTAACAGGTTGGATGATAAG"/></BindingSite><BindingSite location="1071-1124" boundStrand="0" annealedBases="GGTACCTAACAGGTTGGATGATAAG" meltingTemperature="46"><Component bases="ATAT"/><Component hybridizedRange="1071-1077" bases="GGTACCT"/><Component hybridizedRange="1107-1124" bases="AACAGGTTGGATGATAAG"/></BindingSite><BindingSite location="2544-2564" boundStrand="1" annealedBases="CCTAACAGGTTGGATGATAAG" meltingTemperature="55"><Component bases="ATATGGTA"/><Component hybridizedRange="2544-2564" bases="CCTAACAGGTTGGATGATAAG"/></BindingSite><BindingSite simplified="1" location="1071-1095" boundStrand="0" annealedBases="GGTACCTAACAGGTTGGATGATAAG" meltingTemperature="57"><Component bases="ATAT"/><Component hybridizedRange="1071-1095" bases="GGTACCTAACAGGTTGGATGATAAG"/></BindingSite><BindingSite simplified="1" location="1106-1124" boundStrand="0" annealedBases="TAACAGGTTGGATGATAAG" meltingTemperature="46"><Component bases="ATATGGTACC"/><Component hybridizedRange="1106-1124" bases="TAACAGGTTGGATGATAAG"/></BindingSite><BindingSite simplified="1" location="2544-2564" boundStrand="1" annealedBases="CCTAACAGGTTGGATGATAAG" meltingTemperature="55"><Component bases="ATATGGTA"/><Component hybridizedRange="2544-2564" bases="CCTAACAGGTTGGATGATAAG"/></BindingSite></Primer><Primer recentID="8" name="KROL521 Tn_lnSeqB_R" sequence="TATAAACAGGTTGGATGATAAGTCCCCG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2022-05-25T19:07:54Z"><BindingSite location="1078-1101" boundStrand="0" annealedBases="AACAGGTTGGATGATAAGTCCCCG" meltingTemperature="61"><Component bases="TATA"/><Component hybridizedRange="1078-1101" bases="AACAGGTTGGATGATAAGTCCCCG"/></BindingSite><BindingSite location="1107-1130" boundStrand="0" annealedBases="AACAGGTTGGATGATAAGTCCCCG" meltingTemperature="61"><Component bases="TATA"/><Component hybridizedRange="1107-1130" bases="AACAGGTTGGATGATAAGTCCCCG"/></BindingSite><BindingSite location="2538-2561" boundStrand="1" annealedBases="AACAGGTTGGATGATAAGTCCCCG" meltingTemperature="61"><Component bases="TATA"/><Component hybridizedRange="2538-2561" bases="AACAGGTTGGATGATAAGTCCCCG"/></BindingSite><BindingSite simplified="1" location="1078-1101" boundStrand="0" annealedBases="AACAGGTTGGATGATAAGTCCCCG" meltingTemperature="61"><Component bases="TATA"/><Component hybridizedRange="1078-1101" bases="AACAGGTTGGATGATAAGTCCCCG"/></BindingSite><BindingSite simplified="1" location="1107-1130" boundStrand="0" annealedBases="AACAGGTTGGATGATAAGTCCCCG" meltingTemperature="61"><Component bases="TATA"/><Component hybridizedRange="1107-1130" bases="AACAGGTTGGATGATAAGTCCCCG"/></BindingSite><BindingSite simplified="1" location="2538-2561" boundStrand="1" annealedBases="AACAGGTTGGATGATAAGTCCCCG" meltingTemperature="61"><Component bases="TATA"/><Component hybridizedRange="2538-2561" bases="AACAGGTTGGATGATAAGTCCCCG"/></BindingSite></Primer><Primer recentID="9" name="BioSamA_v2" sequence="AAGACGGCATACGAGATTACGAAGACC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2022-10-16T14:44:08Z"><BindingSite location="1130-1147" boundStrand="1" annealedBases="AAGACGGCTACGAAGACC" meltingTemperature="43"><Component hybridizedRange="1140-1147" bases="AAGACGGC"/><Component bases="ATACGAGAT"/><Component hybridizedRange="1130-1139" bases="TACGAAGACC"/></BindingSite><BindingSite location="2520-2538" boundStrand="0" annealedBases="AAGACGGCATACGAAGACC" meltingTemperature="45"><Component hybridizedRange="2520-2528" bases="AAGACGGCA"/><Component bases="TACGAGAT"/><Component hybridizedRange="2529-2538" bases="TACGAAGACC"/></BindingSite><BindingSite simplified="1" location="1130-1147" boundStrand="1" annealedBases="AAGACGGCTACGAAGACC" meltingTemperature="43"><Component hybridizedRange="1140-1147" bases="AAGACGGC"/><Component bases="ATACGAGAT"/><Component hybridizedRange="1130-1139" bases="TACGAAGACC"/></BindingSite><BindingSite simplified="1" location="2520-2538" boundStrand="0" annealedBases="AAGACGGCATACGAAGACC" meltingTemperature="45"><Component hybridizedRange="2520-2528" bases="AAGACGGCA"/><Component bases="TACGAGAT"/><Component hybridizedRange="2529-2538" bases="TACGAAGACC"/></BindingSite></Primer><Primer recentID="10" name="LIB_PCR_5" sequence="CAAGCAGAAGACGGCATACGAAGACCGGGGACTTATCATCCAACCTGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2022-10-16T14:49:23Z"><BindingSite location="1079-1154" boundStrand="1" annealedBases="CAAGCAGAAGACGGCTACGAAGACCGGGGACTTATCATCCAACCTGT" meltingTemperature="63"><Component hybridizedRange="1140-1154" bases="CAAGCAGAAGACGGC"/><Component bases="A"/><Component hybridizedRange="1122-1139" bases="TACGAAGACCGGGGACTT"/><Component hybridizedRange="1079-1092" bases="ATCATCCAACCTGT"/></BindingSite><BindingSite location="1108-1154" boundStrand="1" annealedBases="CAAGCAGAAGACGGCTACGAAGACCGGGGACTTATCATCCAACCTGT" meltingTemperature="68"><Component hybridizedRange="1140-1154" bases="CAAGCAGAAGACGGC"/><Component bases="A"/><Component hybridizedRange="1108-1139" bases="TACGAAGACCGGGGACTTATCATCCAACCTGT"/></BindingSite><BindingSite location="2513-2560" boundStrand="0" annealedBases="CAAGCAGAAGACGGCATACGAAGACCGGGGACTTATCATCCAACCTGT" meltingTemperature="72"><Component hybridizedRange="2513-2560" bases="CAAGCAGAAGACGGCATACGAAGACCGGGGACTTATCATCCAACCTGT"/></BindingSite><BindingSite simplified="1" location="1079-1154" boundStrand="1" annealedBases="CAAGCAGAAGACGGCTACGAAGACCGGGGACTTATCATCCAACCTGT" meltingTemperature="63"><Component hybridizedRange="1140-1154" bases="CAAGCAGAAGACGGC"/><Component bases="A"/><Component hybridizedRange="1122-1139" bases="TACGAAGACCGGGGACTT"/><Component hybridizedRange="1079-1092" bases="ATCATCCAACCTGT"/></BindingSite><BindingSite simplified="1" location="1108-1154" boundStrand="1" annealedBases="CAAGCAGAAGACGGCTACGAAGACCGGGGACTTATCATCCAACCTGT" meltingTemperature="68"><Component hybridizedRange="1140-1154" bases="CAAGCAGAAGACGGC"/><Component bases="A"/><Component hybridizedRange="1108-1139" bases="TACGAAGACCGGGGACTTATCATCCAACCTGT"/></BindingSite><BindingSite simplified="1" location="2513-2560" boundStrand="0" annealedBases="CAAGCAGAAGACGGCATACGAAGACCGGGGACTTATCATCCAACCTGT" meltingTemperature="72"><Component hybridizedRange="2513-2560" bases="CAAGCAGAAGACGGCATACGAAGACCGGGGACTTATCATCCAACCTGT"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>04a8f54f-8731-4ac3-92b2-95e77929704e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>-</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>-</Description>
<Created UTC="4:0:0">2006.10.7</Created>
<LastModified UTC="16:54:56">2023.3.19</LastModified>
<AccessionNumber>-</AccessionNumber>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         6+ Cutters                                                                                                   d     CHO  d               E              Y? P                                                                                                                                                              )<?xml version="1.0"?><CustomEnzymeSets/>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
