LOCUS                               4582 bp ss-DNA     circular SYN 07-Oct-2006
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     misc_feature    complement(1..861)
                     /note="beta-lactamase"
     promoter        complement(896..902)
                     /note="P3"
     misc_feature    complement(1062..1067)
                     /note="MluI"
     frag            1072..1076
                     /note="KpnI"
     misc_feature    2691..2696
                     /note="NdeI"
     gene            2694..3740
                     /note="mariner transposase himar1C9"
     misc_feature    3741..3748
                     /note="NotI"
     misc_feature    complement(3769..4542)
                     /note="RP4-OriT-OriR6K"
     misc_feature    complement(4543..4548)
                     /note="MluI"
     PCR_primer      2..26
                     /note="P497 Seq_pSam_Bt2_1"
     PCR_primer      593..613
                     /note="P498 Seq_pSam_Bt2_2"
     PCR_primer      3112..3136
                     /note="P503 Seq_pSam_Bt2_7"
     PCR_primer      3663..3690
                     /note="P504 Seq_pSam_Bt2_8"
     misc_feature    4199..4518
                     /note="RP4-OriT-OriR6K"
     PCR_primer      complement(4450..4474)
                     /note="P505 Seq_pSam_Bt2_9"
     frag            1078..2535
                     /note="transposon"
     frag            1078..1106
                     /note="MmeI IR_L"
     frag            complement(1084..1089)
                     /note="MmeI"
     primer_bind     1102..1127
                     /note="BioSamA"
     frag            complement(1107..1126)
                     /note="P7"
     frag            1127..1134
                     /note="AscI"
     frag            1135..1140
                     /note="XhoI"
     PCR_primer      1155..1182
                     /note="P052 groESpromoF"
     promoter        complement(1155..1392)
                     /note="groES promoter from LVS"
     PCR_primer      complement(1159..1186)
                     /note="P457 S_pSD26_1"
     PCR_primer      1146..1182
                     /note="P508 KanCass_F"
     CDS             1393..2205
                     /note="kanamycin resistance (aphA1 from Tn903)"
     primer_bind     1375..1409
                     /note="SD38 (do not have)"
     frag            1149..1154
                     /note="MfeI"
     PCR_primer      1548..1572
                     /note="P442 pEX_kanR_F"
     primer_bind     complement(1872..1896)
                     /note="P027 pEX18KanSeq3F"
     PCR_primer      complement(1820..1842)
                     /note="P443 pEX_kanR_R"
     frag            2229..2472
                     /note="3957 to 4200 of pFW11"
     terminator      2259..2302
                     /note="E. coli rrnB terminator T1"
     terminator      2434..2461
                     /note="E. coli rrnB terminator T2"
     misc_feature    2473..2478
                     /note="PstI"
     frag            complement(2479..2486)
                     /note="AscI"
     primer_bind     2486..2511
                     /note="BioSamA"
     frag            2487..2506
                     /note="P7"
     PCR_primer      2241..2265
                     /note="P501 Seq_pSam_Bt2_5"
     PCR_primer      2175..2199
                     /note="P458 S_pSD26_2"
     PCR_primer      complement(2182..2223)
                     /note="P509 KanCass_R"
     misc_feature    2206..2208
                     /note="pSD-P472"
     misc_feature    2215..2220
                     /note="NheI"
     misc_feature    2209..2214
                     /note="NcoI"
     misc_feature    2223..2228
                     /note="XbaI"
     frag            2507..2535
                     /note="MmeI IR_R"
     frag            2524..2524
                     /note="G->T creates MmeI site"
     frag            2524..2529
                     /note="MmeI"
     frag            complement(2536..2541)
                     /note="BamHI"
     frag            1077..1077
                     /note="KpnI"
     promoter        2551..2600
                     /note="NEOKAN promoter from pCR2.1"
     PCR_primer      2542..2564
                     /note="P547 pNEOKAN-F"
     PCR_primer      complement(2657..2696)
                     /note="P548 pNEOKAN-R"
     RBS             2682..2687
                     /note="RBS"
     source          2542..2681
                     /dnas_title="pKL91_pSAM_Ft_PNEOKAN"
                     /note="pF"
ORIGIN      
        1 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
       61 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC
      121 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA
      181 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
      241 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
      301 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT
      361 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC
      421 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT
      481 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
      541 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
      601 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT
      661 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC
      721 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG
      781 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
      841 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
      901 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT
      961 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC
     1021 ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT CACGCGTCCT CGGTACCTAA
     1081 CAGGTTGGAT GATAAGTCCC CGGTCTTCGT ATGCCGTCTT CTGCTTGGCG CGCCCTCGAG
     1141 CAATTATCCA ATTGtatacc cttcaagctt tgaaaaataa acttaattat tatatatgtt
     1201 atttagctag tttttttaat taaagttaaa atcgagagct tgtttgacaa aaaaacaaaa
     1261 aaatttcttg aaaatttttt ttttgactca atatctagac ttgcaagagc ttggaacttt
     1321 gagattgttc taagatgcat acaaattcaa aatgcttaaa caaaaataat ttaacaaagg
     1381 agtaagATTG TTATGAGCCA TATTCAACGG GAAACGTCTT GCTCGAGGCC GCGATTAAAT
     1441 TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT CGGGCAATCA
     1501 GGTGCGACAA TCTATCGATT GTATGGGAAG CCCGATGCGC CAGAGTTGTT TCTGAAACAT
     1561 GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA CTGGCTGACG
     1621 GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA TGCATGGTTA
     1681 CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA TCCTGATTCA
     1741 GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC GATTCCTGTT
     1801 TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA ATCACGAATG
     1861 AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG GCCTGTTGAA
     1921 CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT CGTCACTCAT
     1981 GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG TTGTATTGAT
     2041 GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG GAACTGCCTC
     2101 GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT TGATAATCCT
     2161 GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAACC ATGGGCTAGC
     2221 GATCTAGAAT GCGAGAGTAG GGAACTGCCA GGCATCAAAT AAAACGAAAG GCTCAGTCGA
     2281 AAGACTGGGC CTTTCGTTTT ATCTGTTGTT TGTCGGTGAA CGCTCTCCTG AGTAGGACAA
     2341 ATCCGCCGGG AGCGGATTTG AACGTTGCGA AGCAACGGCC CGGAGGGTGG CGGGCAGGAC
     2401 GCCCGCCATA AACTGCCAGG CATCAAATTA AGCAGAAGGC CATCCTGACG GATGGCCTTT
     2461 TTGCGTTTCT ACCTGCAGGG CGCGCCAAGC AGAAGACGGC ATACGAAGAC CGGGGACTTA
     2521 TCATCCAACC TGTTAGGATC CGCAAGCGAA CCGGAATTGC CAGCTGGGGC GCCCTCTGGT
     2581 AAGGTTGGGA AGCCCTGCAA AGTAAACTGG ATGGCTTTCT TGCCGCCAAG GATCTGATGG
     2641 CGCAGGGGAT CAAGATCTGA TCAAGAGACA GGATGAGGAT Caggaggtaa CATATGGAAA
     2701 AAAAGGAATT TCGTGTTTTG ATAAAATACT GTTTTCTGAA GGGAAAAAAT ACAGTGGAAG
     2761 CAAAAACTTG GCTTGATAAT GAGTTTCCGG ACTCTGCCCC AGGGAAATCA ACAATAATTG
     2821 ATTGGTATGC AAAATTCAAG CGTGGTGAAA TGAGCACGGA GGACGGTGAA CGCAGTGGAC
     2881 GCCCGAAAGA GGTGGTTACC GACGAAAACA TCAAAAAAAT CCACAAAATG ATTTTGAATG
     2941 ACCGTAAAAT GAAGTTGATC GAGATAGCAG AGGCCTTAAA GATATCAAAG GAACGTGTTG
     3001 GTCATATCAT TCATCAATAT TTGGATATGC GGAAGCTCTG TGCGAAATGG GTGCCGCGCG
     3061 AGCTCACATT TGACCAAAAA CAACGACGTG TTGATGATTC TAAGCGGTGT TTGCAGCTGT
     3121 TAACTCGTAA TACACCCGAG TTTTTCCGTC GATATGTGAC AATGGATGAA ACATGGCTCC
     3181 ATCACTACAC TCCTGAGTCC AATCGACAGT CGGCTGAGTG GACAGCGACC GGTGAACCGT
     3241 CTCCGAAGCG TGGAAAGACT CAAAAGTCCG CTGGCAAAGT AATGGCCTCT GTTTTTTGGG
     3301 ATGCGCATGG AATAATTTTT ATCGATTATC TTGAGAAGGG AAAAACCATC AACAGTGACT
     3361 ATTATATGGC GTTATTGGAG CGTTTGAAGG TCGAAATCGC GGCAAAACGG CCCCACATGA
     3421 AGAAGAAAAA AGTGTTGTTC CACCAAGACA ACGCACCGTG CCACAAGTCA TTGAGAACGA
     3481 TGGCAAAAAT TCATGAATTG GGCTTCGAAT TGCTTCCCCA CCCGCCGTAT TCTCCAGATC
     3541 TGGCCCCCAG CGACTTTTTC TTGTTCTCAG ACCTCAAAAG GATGCTCGCA GGGAAAAAAT
     3601 TTGGCTGCAA TGAAGAGGTG ATCGCCGAAA CTGAGGCCTA TTTTGAGGCA AAACCGAAGG
     3661 AGTACTACCA AAATGGTATC AAAAAATTGG AAGGTCGTTA TAATCGTTGT ATCGCTCTTG
     3721 AAGGGAACTA TGTTGAATAA GCGGCCGCCA CCGCGGTGGA GGGGAATTCC CATGTCAGCC
     3781 GTTAAGTGTT CCTGTGTCAC TCAAAATTGC TTTGAGAGGC TCTAAGGGCT TCTCAGTGCG
     3841 TTACATCCCT GGCTTGTTGT CCACAACCGT TAAACCTTAA AAGCTTTAAA AGCCTTATAT
     3901 ATTCTTTTTT TTCTTATAAA ACTTAAAACC TTAGAGGCTA TTTAAGTTGC TGATTTATAT
     3961 TAATTTTATT GTTCAAACAT GAGAGCTTAG TACGTGAAAC ATGAGAGCTT AGTACGTTAG
     4021 CCATGAGAGC TTAGTACGTT AGCCATGAGG GTTTAGTTCG TTAAACATGA GAGCTTAGTA
     4081 CGTTAAACAT GAGAGCTTAG TACGTGAAAC ATGAGAGCTT AGTACGTACT ATCAACAGGT
     4141 TGAACTGCTG ATCTTCAGAT CCTCTACGCC GGACGCATCG TGGCCGGATC AATTCCGTGA
     4201 TAGGTGGGCT GCCCTTCCTG GTTGGCTTGG TTTCATCAGC CATCCGCTTG CCCTCATCTG
     4261 TTACGCCGGC GGTAGCCGGC CAGCCTCGCA GAGCAGGATT CCCGTTGAGC ACCGCCAGGT
     4321 GCGAATAAGG GACAGTGAAG AAGGAACACC CGCTCGCGGG TGGGCCTACT TCACCTATCC
     4381 TGCCCGGCTG ACGCCGTTGG ATACACCAAG GAAAGTCTAC ACGAACCCTT TGGCAAAATC
     4441 CTGTATATCG TGCGAAAAAG GATGGATATA CCGAAAAAAT CGCTATAATG ACCCCGAAGC
     4501 AGGGTTATGC AGCGGAAAAC GGAATTGATC CCCCTCGAAT TGACGCGT
//

