LOCUS       pKL85                   8301 bp    DNA     circular     21-JUL-2018
pEX18KanSequenced.fa -
FEATURES             Location/Qualifiers
     primer_bind     2117..2132
                     /note="P028 pEX18KanSeq4F (not entire 5' end)"
     primer_bind     4390..4416
                     /note="P031 pEX18KanSeq7F"
     primer_bind     5113..5137
                     /note="P033 pEX18KanSeq8F"
     primer_bind     6013..6036
                     /note="P023 pEX18SeqF2"
     primer_bind     6037..6056
                     /note="P021 pEX18SeqF1"
     primer_bind     complement(7604..7629)
                     /note="P024 pEX18SeqR2"
     primer_bind     complement(7626..7645)
                     /note="P022 pEX18SeqR1"
     CDS             complement(2707..4125)
                     /note="sacB"
     primer_bind     4..26
                     /note="P025 pEX18KanSeq1F"
     primer_bind     474..493
                     /note="P037 pEX18KanSeq9F"
     promoter        866..1103
                     /note="groES promotoer"
     primer_bind     733..755
                     /note="P026 pEX18KanSeq2F"
     CDS             1104..1916
                     /note="kanamycin resistance"
     primer_bind     complement(1583..1607)
                     /note="P027 pEX18KanSeq3F"
     rep_origin      complement(join(8001..8301,1..288))
                     /note="ColE origin"
     primer_bind     2968..2994
                     /note="P029 pEX18KanSeq5F"
     primer_bind     3649..3675
                     /note="P030 pEX18KanSeq6F"
     gene            complement(6555..6563)
                     /locus_tag="FTL_0136"
                     /db_xref="GeneID:3951744"
     CDS             complement(6555..6563)
                     /dnas_title="hypothetical protein"
                     /locus_tag="FTL_0136"
                     /inference="ab initio prediction"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_512934.1"
                     /db_xref="GI:89255573"
                     /db_xref="GeneID:3951744"
                     /translation="LWTYVNNVFQQTKNQTPIENNLSIINNPIFDVFLPVQNISKIGN
                     STASAHVVTRYKDKKNEKVNYFVKSLNNNEVENAIAEVIYAQIWNYFIGNRASKSLLL
                     SKHENKIIGIASKGINDFQEYKSLSGDQKDYPGLIQILFYVCVLIENDFHICNFGTGL
                     FNGKRCYSKIDHDYIVSFWDALKYGKFMTEVSHNFQELFKKNLCLVYFFF"
     misc_feature    complement(6611..6611)
                     /note="Putative TSS"
     misc_feature    complement(6625..6625)
                     /note="RNA-Seq potential TSS"
     PCR_primer      complement(7301..7328)
                     /note="P516 DFTL_0136_2R"
                     /current=0
     PCR_primer      complement(7555..7589)
                     /note="P514 DopiA_2R"
                     /current=0
     misc_feature    complement(6573..6581)
                     /note=""
     gene            complement(6932..7583)
                     /locus_tag="FTL_0137"
                     /db_xref="GeneID:3951745"
     CDS             complement(6932..7583)
                     /dnas_title="lipopolysaccharide protein"
                     /locus_tag="FTL_0137"
                     /inference="similar to AA sequence:INSD:Uniprot"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide protein"
                     /protein_id="YP_512935.1"
                     /db_xref="GI:89255574"
                     /db_xref="GeneID:3951745"
                     /translation="MKKLKYYIKNISRTIIPKKFFELSLEHKLKSITKFDKEYIEYRV
                     NYYNRLSKPFMLQNSSNWKNFKRNTPVFDIIDHSLVRKSINSSYFYDFKEFLVYFNKT
                     DSFATAFYDLTKIPQQPTFVKSRPIADDNQNSIILKLDKLRHFSLFEDNQKFEDKLNM
                     AVFRGACHQPTRQYFIENYYNLPNTNFGDTRKESIGQPYNKGFLSIQDQLKYKYIVSI
                     EGYDVATNLKWIMNSNSLCFMNKPKYETWFMEGTLIPNHHYVLLKDDYSDLQEKIDYY
                     NNHPEKALKIIKNANEYVNQFKNKQREELISLLVMKNYFGLNKTSH"
     PCR_primer      6107..6140
                     /note="P511 DopiA_1F"
     PCR_primer      complement(6537..6571)
                     /note="P512 DopiA_1R"
     PCR_primer      6564..6602
                     /note="P513 DopiA_2F"
     PCR_primer      6493..6521
                     /note="P520 SeqDopiA_1"
     source          1..8301
                     /dnas_title="pKL85 pEXDopiA"
ORIGIN      
        1 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT
       61 AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT
      121 AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC
      181 TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT
      241 TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG
      301 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
      361 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
      421 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAATC GATCAAGGGT TGGTTTGCGC
      481 ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC
      541 GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC
      601 GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG
      661 CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAATGATCGA AGTTAGGCTG
      721 GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC
      781 AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG
      841 AAGGCCATCC AGCCTCGCGT CGATCTATAC CCTTCAAGCT TTGAAAAATA AACTTAATTA
      901 TTATATATGT TATTTAGCTA GTTTTTTTAA TTAAAGTTAA AATCGAGAGC TTGTTTGACA
      961 AAAAAACAAA AAAATTTCTT GAAAATTTTT TTTTTGACTC AATATCTAGA CTTGCAAGAG
     1021 CTTGGAACTT TGAGATTGTT CTAAGATGCA TACAAATTCA AAATGCTTAA ACAAAAATAA
     1081 TTTAACAAAG GAGTAAGATT GTTATGAGCC ATATTCAACG GGAAACGTCT TGCTCGAGGC
     1141 CGCGATTAAA TTCCAACATG GATGCTGATT TATATGGGTA TAAATGGGCT CGCGATAATG
     1201 TCGGGCAATC AGGTGCGACA ATCTATCGAT TGTATGGGAA GCCCGATGCG CCAGAGTTGT
     1261 TTCTGAAACA TGGCAAAGGT AGCGTTGCCA ATGATGTTAC AGATGAGATG GTCAGACTAA
     1321 ACTGGCTGAC GGAATTTATG CCTCTTCCGA CCATCAAGCA TTTTATCCGT ACTCCTGATG
     1381 ATGCATGGTT ACTCACCACT GCGATCCCCG GAAAAACAGC ATTCCAGGTA TTAGAAGAAT
     1441 ATCCTGATTC AGGTGAAAAT ATTGTTGATG CGCTGGCAGT GTTCCTGCGC CGGTTGCATT
     1501 CGATTCCTGT TTGTAATTGT CCTTTTAACA GCGATCGCGT ATTTCGTCTC GCTCAGGCGC
     1561 AATCACGAAT GAATAACGGT TTGGTTGATG CGAGTGATTT TGATGACGAG CGTAATGGCT
     1621 GGCCTGTTGA ACAAGTCTGG AAAGAAATGC ATAAACTTTT GCCATTCTCA CCGGATTCAG
     1681 TCGTCACTCA TGGTGATTTC TCACTTGATA ACCTTATTTT TGACGAGGGG AAATTAATAG
     1741 GTTGTATTGA TGTTGGACGA GTCGGAATCG CAGACCGATA CCAGGATCTT GCCATCCTAT
     1801 GGAACTGCCT CGGTGAGTTT TCTCCTTCAT TACAGAAACG GCTTTTTCAA AAATATGGTA
     1861 TTGATAATCC TGATATGAAT AAATTGCAGT TTCATTTGAT GCTCGATGAG TTTTTCTAAT
     1921 CAGAATTGGT TAATTGGTTG TAACACTGGC AGAGCATTAC GCTGACTTGA CGGGACGGCG
     1981 GCTTTGTTGA ATAAATCGAA CTTTTGCTGA GTTGAAGGAT CAGATCACGC ATCTTCCCGA
     2041 CAACGCAGAC CGTTCCGTGG CAAAGCAAAA GTTCAAAATC ACCAACTGGT CCACCTACAA
     2101 CAAAGCTCTC ATCAACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA
     2161 TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA
     2221 TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTAAT
     2281 CGGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG
     2341 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAGAAAT AAACAAATAG GGGTTCCGCG
     2401 CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC
     2461 CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA
     2521 AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG
     2581 GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA
     2641 CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT AATCGGCATT TTCTTTTGCG
     2701 TTTTTATTTG TTAACTGTTA ATTGTCCTTG TTCAAGGATG CTGTCTTTGA CAACAGATGT
     2761 TTTCTTGCCT TTGATGTTCA GCAGGAAGCT AGGCGCAAAC GTTGATTGTT TGTCTGCGTA
     2821 GAATCCTCTG TTTGTCATAT AGCTTGTAAT CACGACATTG TTTCCTTTCG CTTGAGGTAC
     2881 AGCGAAGTGT GAGTAAGTAA AGGTTACATC GTTAGGATCA AGATCCATTT TTAACACAAG
     2941 GCCAGTTTTG TTCAGCGGCT TGTATGGGCC AGTTAAAGAA TTAGAAACAT AACCAAGCAT
     3001 GTAAATATCG TTAGACGTAA TGCCGTCAAT CGTCATTTTT GATCCGCGGG AGTCAGTGAA
     3061 CAGATACCAT TTGCCGTTCA TTTTAAAGAC GTTCGCGCGT TCAATTTCAT CTGTTACTGT
     3121 GTTAGATGCA ATCAGCGGTT TCATCACTTT TTTCAGTGTG TAATCATCGT TTAGCTCAAT
     3181 CATACCGAGA GCGCCGTTTG CTAACTCAGC CGTGCGTTTT TTATCGCTTT GCAGAAGTTT
     3241 TTGACTTTCT TGACGGAAGA ATGATGTGCT TTTGCCATAG TATGCTTTGT TAAATAAAGA
     3301 TTCTTCGCCT TGGTAGCCAT CTTCAGTTCC AGTGTTTGCT TCAAATACTA AGTATTTGTG
     3361 GCCTTTATCT TCTACGTAGT GAGGATCTCT CAGCGTATGG TTGTCGCCTG AGCTGTAGTT
     3421 GCCTTCATCG ATGAACTGCT GTACATTTTG ATACGTTTTT CCGTCACCGT CAAAGATTGA
     3481 TTTATAATCC TCTACACCGT TGATGTTCAA AGAGCTGTCT GATGCTGATA CGTTAACTTG
     3541 TGCAGTTGTC AGTGTTTGTT TGCCGTAATG TTTACCGGAG AAATCAGTGT AGAATAAACG
     3601 GATTTTTCCG TCAGATGTAA ATGTGGCTGA ACCTGACCAT TCTTGTGTTT GGTCTTTTAG
     3661 GATAGAATCA TTTGCATCGA ATTTGTCGCT GTCTTTAAAG ACGCGGCCAG CGTTTTTCCA
     3721 GCTGTCAATA GAAGTTTCGC CGACTTTTTG ATAGAACATG TAAATCGATG TGTCATCCGC
     3781 ATTTTTAGGA TCTCCGGCTA ATGCAAAGAC GATGTGGTAG CCGTGATAGT TTGCGACAGT
     3841 GCCGTCAGCG TTTTGTAATG GCCAGCTGTC CCAAACGTCC AGGCCTTTTG CAGAAGAGAT
     3901 ATTTTTAATT GTGGACGAAT CGAACTCAGG AACTTGATAT TTTTCATTTT TTTGCTGTTC
     3961 AGGGATTTGC AGCATATCAT GGCGTGTAAT ATGGGAAATG CCGTATGTTT CCTTATATGG
     4021 CTTTTGGTTC GTTTCTTTCG CAAACGCTTG AGTTGCGCCT CCTGCCAGCA GTGCGGTAGT
     4081 AAAGGTTAAT ACTGTTGCTT GTTTTGCAAA CTTTTTGATG TTCATCGTTC ATGTCTCCTT
     4141 TTTTATGTAC TGTGTTAGCG GTCTGCTTCT TCCAGCCCTC CTGTTTGAAG ATGGCAAGTT
     4201 AGTTACGCAC AATAAAAAAA GACCTAAAAT ATGTAAGGGG TGACGCCAAA GTATACACTT
     4261 TGCCCTTTAC ACATTTTAGG TCTTGCCTGC TTTATCAGTA ACAAACCCGC GCGATTTACT
     4321 TTTCGACCTC ATTCTATTAG ACTCTCGTTT GGATTGCAAC TGGTCTATTT TCCTCTTTTG
     4381 TTTGATAGAA AATCATAAAA GGATTTGCAG ACTACGGGCC TAAAGAACTA AAAAATCTAT
     4441 CTGTTTCTTT TCATTCTCTG -TATTTTTTA TAGTTTCTGT TGCATGGGCA TAAAGTTGCC
     4501 TTTTTAATCA CAATTCAGAA AATATCATAA TATCTCATTT CACTAAATAA TAGTGAACGG
     4561 CAGGTATATG TGATGGGTTA AAAAGGATCG ATCCTCTAGC TAGAGTCGAT CTTCGCCAGC
     4621 AGGGCGAGGA TCGTGGCATC ACCGAA-CCG CGCCGTGCGC GGGTCGTCGG TGAGCCAGAG
     4681 TTTCAGCAGG CCGCCCAGGC GGCCCAGGTC GCCATTGATG CGGGCCAGCT CGCGGACGTG
     4741 CTCATAGTCC ACGACGCCCG TGATTTTGTA GCCCTGGCCG ACGGCCAGCA GGTAGGCCGA
     4801 CAGGCTCATG CCGGCTGCCG CCGCCTTTTC CTCAATCGCT CTTCGTTCGT CTGGAAGGCA
     4861 GTACACCTTG ATAGGTGGGC TGCCCTTCCT GGTTGGCTTG GTTTCATCAG CCATCCGCTT
     4921 GCCCTCATCT GTTA-CGCCG GCGGTAGCCG GCCAGCCTCG CAGAGCAGGA TTCCCGTTGA
     4981 GCACCGCCAG GTGCGAATAA GGGACAGTGA AGAAGGAACA CCCGCTCGCG GGTGGGCCTA
     5041 CTTCACCTA- TCCTGCCCGG CTGACGCCGT TGGATACACC AAGGAAAGTC TACACGAACC
     5101 CTTTGGCAAA ATCCTGTATA TCGTGCGAAA AAGGATGGAT ATACCGAAAA AATCGCTATA
     5161 ATGACCCCGA AGCAGGGTTA TGCAGCGGAA AAGCGCTGCT TCCCTGCTGT TTTGTGGAAT
     5221 ATCTACCGAC TGGAAACAGG CAAATGCAGG AAATTACTGA ACTGAGGGGA CAGGCGAGAG
     5281 ACGATGCCAA AGAGCTACAC CGACGAGCTG GCCGAGTGGG TTGAATCCCG CGCGGCCAAG
     5341 AAGCGCCGGC GTGATGAGGC TGCGGTTGCG TTCCTGGCGG TGAGGGCGGA TGTCGATATG
     5401 CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG CATATTTGAA
     5461 -TGTATTTAG AAAAATAAAC AAAAGAGTTT GTAGAAACGC AAAAAGGCCA TCCGTCAGGA
     5521 TGGCCTTCTG CTTAATTTGA TGCCTGGCAG TTTATGGCGG GCGTCCTGCC CGCCACCCTC
     5581 CGGGCCGTTG CTTCGCAACG TTCAAATCCG CTCCCGGCGG ATTTGTCCTA CTCAGGAGAG
     5641 CGTTCACCGA CAAACAACAG ATAAAACGAA AGGCCCAGTC TTTCGACTGA GCCTTTCGTT
     5701 TTATTTGATG CCTGGCAGTT CCCTACTCTC GCATGGGGAG ACCCCACACT ACCATCGGCG
     5761 CTACGGCGTT TCACTTCTGA GTTCGGCATG GGGTCAGGTG GGACCACCGC GCTACTGCCG
     5821 CCAGGCAAAT TCTGTTTTAT CAGACCGCTT CTGCGTTCTG ATTTAATCTG TATCAGGCTG
     5881 AAAATCTTCT CTCATCCGCC AAAACAGCCA AGCTCGCCAT TCGCCATTCA GGCTGCGCAA
     5941 CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCTGG CGAAAGGGGG
     6001 ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA
     6061 AACGACGGCC AGTGCCAAGC TTGCATGCCT GCAGGTCGAC TCTAGAGGAT CCtatctttc
     6121 ggattaacta tatctatgag taacctagta ttagtttaga gaatgtcagc atttagtagg
     6181 tagaaaataa aataaagtag tattaagatt aattcaacaa attacacaaa tgagaaaatc
     6241 ttaatttgaa aaagaataat aactcattgg atttacctaa gtatttatag ttatactgaa
     6301 taaggggagc gattttagtc attaaaactc cttcaagatc tgaaaaaatc tactaatttc
     6361 agcaggtcta gttttaagaa taaaatcatt tattgtatat tcaagttctt tttgattact
     6421 cctggttatt gccttatttc gaaattgact catcatcata tgtgaagtaa ctgttgacgg
     6481 tcttccaaga cgtattttat gacctaattc taagattctt gaatttgctt taacaggact
     6541 aaaacgcatt gattctaaaa gaaGCGGCCG CCaaaatctt ttcaaaattt ttcatagact
     6601 agcatacagt taagactttg aggtcaatac atgatatcaa taaataattt tctctacaag
     6661 ctagtgttat agcgacttat aattataaac cttatatcaa cactttgatt tagctataat
     6721 agatatatca aggtaattta gcgcatctta tacaagaaga attttgctta agaaacttca
     6781 tctatagact agattttcaa caaattatat atttttcttc ggttattagg ttctatgaat
     6841 aaaaaatatt tcagttaaat aagtaaatca actctaaagc tgtagagaaa aatcttatca
     6901 aatccagaaa acattcttat aaaattaagc tctagtgaga cgttttattt aaaccaaaat
     6961 aattcttcat aacaagtaaa gaaatcagtt cttctctttg tttatttttg aattgattta
     7021 catattcgtt tgcattttta attattttaa gagctttttc ggggtgattg ttataatagt
     7081 ctattttctc ttgaagatca gaataatcat cttttaataa tacatagtga tgatttggta
     7141 ttagggtacc ttccataaac cacgtttcat attttggttt attcataaaa caaagggaat
     7201 ttgagttcat gatccattta agattagtag caacatcgta gccctctata ctaacgatat
     7261 atttatattt aagctggtct tgtatgctca gaaagccttt gttatatggt tgaccaattg
     7321 attctttacg cgtgtcacca aaatttgtat taggtaagtt ataataattt tctatgaaat
     7381 attgtcgagt gggttgatga caagctcctc taaagactgc catattaagt ttatcttcaa
     7441 atttttgatt gtcttcaaaa aggctaaagt gtcgtagttt atcaagcttt agaataatag
     7501 agttttgatt atcatcagct attggtctac tttttacaaa agttggttgt tggggtattt
     7561 tagtaagatc ataaaaagct gtaGAATTCG TAATCATGTC ATAGCTGTTT CCTGTGTGAA
     7621 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT
     7681 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC
     7741 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG
     7801 GTTTGCGTAT TGGGCGCTCT TCCGCT-TCC TCGCTCACTG ACCCGCTGCG CTCGGTCGTT
     7861 CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA
     7921 GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA
     7981 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT
     8041 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC
     8101 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC
     8161 GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT
     8221 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC
     8281 CGCTGCGCCT TATCCGGTAA C
//

