LOCUS       pKL75                   5676 bp    DNA     circular     21-JUL-2018
pEX18KanSequenced.fa -
FEATURES             Location/Qualifiers
     primer_bind     2117..2119
                     /note="P028 pEX18KanSeq4F (not entire 5' end)"
     primer_bind     2648..2674
                     /note="P031 pEX18KanSeq7F"
     primer_bind     3367..3391
                     /note="P033 pEX18KanSeq8F"
     primer_bind     4266..4289
                     /note="P023 pEX18SeqF2"
     CDS             complement(2120..2383)
                     /note="sacB"
     primer_bind     4..26
                     /note="P025 pEX18KanSeq1F"
     primer_bind     474..493
                     /note="P037 pEX18KanSeq9F"
     promoter        866..1103
                     /note="groES promotoer"
     primer_bind     733..755
                     /note="P026 pEX18KanSeq2F"
     CDS             1104..1916
                     /note="kanamycin resistance"
     primer_bind     complement(1583..1607)
                     /note="P027 pEX18KanSeq3F"
     rep_origin      complement(join(5376..5676,1..288))
                     /note="ColE origin"
     misc_feature    859..883
                     /note="P049 groES promoter F"
     primer_bind     4290..4309
                     /note="P021 pEX18SeqF1"
     primer_bind     complement(5002..5021)
                     /note="P022 pEX18SeqR1"
     primer_bind     complement(4980..5005)
                     /note="P024 pEX18SeqR2"
     sig_peptide     4924..4959
                     /note="VSVG"
     primer_bind     complement(4928..4956)
                     /note="P035 pEX18integrationVSVG"
     misc_feature    join(4375..5676,1..894)
                     /note="Contig 2556 (take1)"
     misc_feature    866..1107
                     /note="Contig 436 (take1)"
     misc_feature    1079..2820
                     /note="Contig 1410 (take1)"
     misc_feature    complement(2819..3061)
                     /note="Contig 678 (take1)"
     misc_feature    3054..3156
                     /note="Contig 2844 (take1)"
     misc_feature    3204..3294
                     /note="Contig 1856 (take1)"
     misc_feature    3306..3551
                     /note="Contig 2784 (take1)"
     misc_feature    complement(join(4375..5676,1..890))
                     /note="Contig 930 (take 2)"
     primer_bind     complement(5140..5163)
                     /note="P227 SP_pKL02_R"
     primer_bind     4931..4960
                     /note="P226 SP_pKL02_F"
     primer_bind     826..859
                     /note="P261 groESpCheck1"
     PCR_primer      complement(2007..2030)
                     /note="P441 pEX_ckR"
     PCR_primer      1259..1283
                     /note="P442 pEX_kanR_F"
     PCR_primer      complement(1531..1553)
                     /note="P443 pEX_kanR_R"
     gene            4375..4914
                     /locus_tag="FTL_0702"
                     /db_xref="GeneID:3951071"
     CDS             4375..4914
                     /dnas_title="hypothetical protein"
                     /locus_tag="FTL_0702"
                     /inference="ab initio prediction"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_513453.1"
                     /db_xref="GI:89256091"
                     /db_xref="GeneID:3951071"
                     /translation="SLISIRTNTKVTTARNIINRLSNKKAVKPILRHLLNQTSIDDII
                     LYDLDKLGDNWALKAATNAIRSTIGSDSILYVKGHTLMLISTSSMVDAFIKAIAENEI
                     YKQVSDADRVLFGKNACNFTAAKNSNNPIVKAMIAASKQIAGQLPKGQVIDTVFEEKV
                     YLALQSTMFENLSNVLPKEE"
     primer_bind     complement(4607..4635)
                     /note="P368 FTL0702seqR1"
     PCR_primer      complement(4538..4562)
                     /note="P411 FTL0702sp_2R"
     PCR_primer      4685..4707
                     /note="P437 RT_FTL0702-2F"
     PCR_primer      complement(4790..4811)
                     /note="P438 RT_FTL0702-2R"
     PCR_primer      4369..4401
                     /note="P444 pKL75_F"
     source          complement(4375..4914)
                     /dnas_title="pKL75 (pEX_FTL_0702-VSVG)"
                     /organism="Francisella tularensis subsp. holarctica"
                     /mol_type="genomic DNA"
                     /strain="LVS"
                     /sub_species="holarctica"
                     /db_xref="taxon:119857"
     PCR_primer      complement(4887..4922)
                     /note="P365 FTL0702cmp_R"
ORIGIN      
        1 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT
       61 AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT
      121 AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC
      181 TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT
      241 TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG
      301 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
      361 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
      421 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAATC GATCAAGGGT TGGTTTGCGC
      481 ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC
      541 GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC
      601 GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG
      661 CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAATGATCGA AGTTAGGCTG
      721 GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC
      781 AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG
      841 AAGGCCATCC AGCCTCGCGT CGATCTATAC CCTTCAAGCT TTGAAAAATA AACTTAATTA
      901 TTATATATGT TATTTAGCTA GTTTTTTTAA TTAAAGTTAA AATCGAGAGC TTGTTTGACA
      961 AAAAAACAAA AAAATTTCTT GAAAATTTTT TTTTTGACTC AATATCTAGA CTTGCAAGAG
     1021 CTTGGAACTT TGAGATTGTT CTAAGATGCA TACAAATTCA AAATGCTTAA ACAAAAATAA
     1081 TTTAACAAAG GAGTAAGATT GTTATGAGCC ATATTCAACG GGAAACGTCT TGCTCGAGGC
     1141 CGCGATTAAA TTCCAACATG GATGCTGATT TATATGGGTA TAAATGGGCT CGCGATAATG
     1201 TCGGGCAATC AGGTGCGACA ATCTATCGAT TGTATGGGAA GCCCGATGCG CCAGAGTTGT
     1261 TTCTGAAACA TGGCAAAGGT AGCGTTGCCA ATGATGTTAC AGATGAGATG GTCAGACTAA
     1321 ACTGGCTGAC GGAATTTATG CCTCTTCCGA CCATCAAGCA TTTTATCCGT ACTCCTGATG
     1381 ATGCATGGTT ACTCACCACT GCGATCCCCG GAAAAACAGC ATTCCAGGTA TTAGAAGAAT
     1441 ATCCTGATTC AGGTGAAAAT ATTGTTGATG CGCTGGCAGT GTTCCTGCGC CGGTTGCATT
     1501 CGATTCCTGT TTGTAATTGT CCTTTTAACA GCGATCGCGT ATTTCGTCTC GCTCAGGCGC
     1561 AATCACGAAT GAATAACGGT TTGGTTGATG CGAGTGATTT TGATGACGAG CGTAATGGCT
     1621 GGCCTGTTGA ACAAGTCTGG AAAGAAATGC ATAAACTTTT GCCATTCTCA CCGGATTCAG
     1681 TCGTCACTCA TGGTGATTTC TCACTTGATA ACCTTATTTT TGACGAGGGG AAATTAATAG
     1741 GTTGTATTGA TGTTGGACGA GTCGGAATCG CAGACCGATA CCAGGATCTT GCCATCCTAT
     1801 GGAACTGCCT CGGTGAGTTT TCTCCTTCAT TACAGAAACG GCTTTTTCAA AAATATGGTA
     1861 TTGATAATCC TGATATGAAT AAATTGCAGT TTCATTTGAT GCTCGATGAG TTTTTCTAAT
     1921 CAGAATTGGT TAATTGGTTG TAACACTGGC AGAGCATTAC GCTGACTTGA CGGGACGGCG
     1981 GCTTTGTTGA ATAAATCGAA CTTTTGCTGA GTTGAAGGAT CAGATCACGC ATCTTCCCGA
     2041 CAACGCAGAC CGTTCCGTGG CAAAGCAAAA GTTCAAAATC ACCAACTGGT CCACCTACAA
     2101 CAAAGCTCTC ATCAACGATC CAGCTGTCCC AAACGTCCAG GCCTTTTGCA GAAGAGATAT
     2161 TTTTAATTGT GGACGAATCG AACTCAGGAA CTTGATATTT TTCATTTTTT TGCTGTTCAG
     2221 GGATTTGCAG CATATCATGG CGTGTAATAT GGGAAATGCC GTATGTTTCC TTATATGGCT
     2281 TTTGGTTCGT TTCTTTCGCA AACGCTTGAG TTGCGCCTCC TGCCAGCAGT GCGGTAGTAA
     2341 AGGTTAATAC TGTTGCTTGT TTTGCAAACT TTTTGATGTT CATCGTTCAT GTCTCCTTTT
     2401 TTATGTACTG TGTTAGCGGT CTGCTTCTTC CAGCCCTCCT GTTTGAAGAT GGCAAGTTAG
     2461 TTACGCACAA TAAAAAAAGA CCTAAAATAT GTAAGGGGTG ACGCCAAAGT ATACACTTTG
     2521 CCCTTTACAC ATTTTAGGTC TTGCCTGCTT TATCAGTAAC AAACCCGCGC GATTTACTTT
     2581 TCGACCTCAT TCTATTAGAC TCTCGTTTGG ATTGCAACTG GTCTATTTTC CTCTTTTGTT
     2641 TGATAGAAAA TCATAAAAGG ATTTGCAGAC TACGGGCCTA AAGAACTAAA AAATCTATCT
     2701 GTTTCTTTTC ATTCTCTGTA TTTTTTATAG TTTCTGTTGC ATGGGCATAA AGTTGCCTTT
     2761 TTAATCACAA TTCAGAAAAT ATCATAATAT CTCATTTCAC TAAATAATAG TGAACGGCAG
     2821 GTATATGTGA TGGGTTAAAA AGGATCGATC CTCTAGCTAG AGTCGATCTT CGCCAGCAGG
     2881 GCGAGGATCG TGGCATCACC GAACCGCGCC GTGCGCGGGT CGTCGGTGAG CCAGAGTTTC
     2941 AGCAGGCCGC CCAGGCGGCC CAGGTCGCCA TTGATGCGGG CCAGCTCGCG GACGTGCTCA
     3001 TAGTCCACGA CGCCCGTGAT TTTGTAGCCC TGGCCGACGG CCAGCAGGTA GGCCGACAGG
     3061 CTCATGCCGG CTGCCGCCGC CTTTTCCTCA ATCGCTCTTC GTTCGTCTGG AAGGCAGTAC
     3121 ACCTTGATAG GTGGGCTGCC CTTCCTGGTT GGCTTGGTTT CATCAGCCAT CCGCTTGCCC
     3181 TCATCTGTTA CGCCGGCGGT AGCCGGCCAG CCTCGCAGAG CAGGATTCCC GTTGAGCACC
     3241 GCCAGGTGCG AATAAGGGAC AGTGAAGAAG GAACACCCGC TCGCGGGTGG GCCTACTTCA
     3301 CCTATCCTGC CCGGCTGACG CCGTTGGATA CACCAAGGAA AGTCTACACG AACCCTTTGG
     3361 CAAAATCCTG TATATCGTGC GAAAAAGGAT GGATATACCG AAAAAATCGC TATAATGACC
     3421 CCGAAGCAGG GTTATGCAGC GGAAAAGCGC TGCTTCCCTG CTGTTTTGTG GAATATCTAC
     3481 CGACTGGAAA CAGGCAAATG CAGGAAATTA CTGAACTGAG GGGACAGGCG AGAGACGATG
     3541 CCAAAGAGCT ACACCGACGA GCTGGCCGAG TGGGTTGAAT CCCGCGCGGC CAAGAAGCGC
     3601 CGGCGTGATG AGGCTGCGGT TGCGTTCCTG GCGGTGAGGG CGGATGTCGA TATGCGGTGT
     3661 GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCATATT TGAATGTATT
     3721 TAGAAAAATA AACAAAAGAG TTTGTAGAAA CGCAAAAAGG CCATCCGTCA GGATGGCCTT
     3781 CTGCTTAATT TGATGCCTGG CAGTTTATGG CGGGCGTCCT GCCCGCCACC CTCCGGGCCG
     3841 TTGCTTCGCA ACGTTCAAAT CCGCTCCCGG CGGATTTGTC CTACTCAGGA GAGCGTTCAC
     3901 CGACAAACAA CAGATAAAAC GAAAGGCCCA GTCTTTCGAC TGAGCCTTTC GTTTTATTTG
     3961 ATGCCTGGCA GTTCCCTACT CTCGCATGGG GAGACCCCAC ACTACCATCG GCGCTACGGC
     4021 GTTTCACTTC TGAGTTCGGC ATGGGGTCAG GTGGGACCAC CGCGCTACTG CCGCCAGGCA
     4081 AATTCTGTTT TATCAGACCG CTTCTGCGTT CTGATTTAAT CTGTATCAGG CTGAAAATCT
     4141 TCTCTCATCC GCCAAAACAG CCAAGCTCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG
     4201 GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT
     4261 GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG
     4321 GCCAGTGCCA AGCTTGCATG CCTGCAGGTC GACTCTAGAG GATCCCCGGG TACCagctta
     4381 atcagtatca gaactaatac caaagttact acagctagaa atattattaa cagactatca
     4441 aataaaaaag ctgtaaaacc tatactaaga cacttactaa atcaaactag tattgatgat
     4501 attatacttt acgacctaga taaattaggc gacaactggg cacttaaagc agctacaaat
     4561 gcaattagat caacaatagg ttctgatagt atactatacg ttaagggtca tactttaatg
     4621 ttaatatcta caagtagtat ggttgatgcc tttatcaagg ctattgctga gaatgaaatt
     4681 tacaagcaag taagtgatgc tgatagagtt ctatttggta aaaatgcatg taattttaca
     4741 gcagctaaaa atagtaacaa tcctatagtt aaagctatga ttgcagcttc taaacagata
     4801 gccggacaac ttcctaaagg tcaagttatc gacaccgttt tcgaagagaa agtatatttg
     4861 gctttacaat caactatgtt tgaaaactta agtaatgttt tgcctaaaga agaaGCGGCC
     4921 GCaTATACTG ATATTGAAAT GAATAGATTA GGTAAATAAG AATTCGTAAT CATGTCATAG
     4981 CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC
     5041 ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC
     5101 TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA
     5161 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACCCG
     5221 CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG
     5281 TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG
     5341 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC
     5401 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA
     5461 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT
     5521 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC
     5581 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC
     5641 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAAC
//

