LOCUS Exported 7614 bp ds-DNA circular SYN 22-JUL-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7614) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 22, 2018 from SnapGene 4.1.9 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7614 /organism="synthetic DNA construct" /note="/dnas_title=pF-PigR-VSVG 2" promoter 299..533 /note="GroE" misc_feature 436..458 /label=nonstandard type: PCR_primer /note="AROL2" misc_feature 594..625 /label=nonstandard type: PCR_primer /note="P346 PigR-VSVG-F" primer_bind 600..621 /label=P041 INTpEX_FTL0449 /note="P041 INTpEX_FTL0449" gene 616..948 /locus_tag="FTL_0449" /label=FTL_0449 /db_xref="GeneID:3952313" CDS 616..948 /codon_start=1 /locus_tag="FTL_0449" /product="hypothetical protein" /label=FTL_0449 /note="/dnas_title=PigR" /db_xref="GI:89255859" /db_xref="GeneID:3952313" /inference="ab initio prediction" /protein_id="YP_513221.1" /translation="MANQYSGNFEQIVKNRFKCSAREILLKCQREGLSYQDAEKVLGFK HVTIRKWAKRFDIKLPPRFRSHDEHLEQKREIAYVNQCKLNKINKRNVFSRCWINMNLY SVIKAKS" misc_feature 671..690 /label=JCOL802 - RT_PCR for PigR /note="JCOL802 - RT_PCR for PigR" misc_feature 750..772 /label=JCOL803 - RT-PCR for PigR /note="JCOL803 - RT-PCR for PigR" misc_feature complement(918..956) /label=nonstandard type: PCR_primer /note="P056 FTL_0449fragR2" gene complement(924..948) /gene="psd" /locus_tag="FTL_0450" /label=psd /db_xref="GeneID:3952314" CDS complement(924..948) /gene="psd" /locus_tag="FTL_0450" /product="phosphatidylserine decarboxylase" /label=psd /note="catalyzes the decarboxylation of phosphatidyl-L-serine to phosphatidylethanoleamine" /note="/dnas_title=phosphatidylserine decarboxylase" /db_xref="GI:89255860" /db_xref="GeneID:3952314" /inference="profile:same species:4.1" /protein_id="YP_513222.1" CDS 958..990 /label=VSV-G /note="VSV-G" misc_feature complement(1119..1141) /label=nonstandard type: PCR_primer /note="AROL3" gene 1284..2159 /label=ORF3 /note="ORF3" gene complement(2382..2801) /label=ORF2 /note="ORF2" gene complement(2807..3823) /label=RepA /note="RepA" gene 5002..5793 /label=kanamycin /note="kanamycin" gene 5814..6671 /label=ampicillin /note="ampicillin" ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 241 gtaccttgta tggattagtc gagctaaaaa gctcatattt tttatattca aactatatac 301 ccttcaagct ttgaaaaata aacttaatta ttatatatgt tatttagcta gtttttttaa 361 ttaaagttaa aatcgagagc ttgtttgaca aaaaaacaaa aaaatttctt gaaaattttt 421 tttttgactc aatatctaga cttgcaagag cttggaactt tgagattgtt ctaagatgca 481 tacaaattca aaatgcttaa acaaaaataa tttaacaaag gagtaagatt gttatgaaca 541 ttcgtccatt acaagataga gtattagttc gtcgtgcagg taccatatga attcccggga 601 aaggagactc gttatatggc gaatcaatat tctggaaatt ttgagcaaat tgtcaaaaat 661 agatttaaat gctcagcgag agagatctta ttaaaatgcc aaagagaagg cttaagttac 721 caagatgctg aaaaagtttt gggttttaag catgttacta tcagaaagtg ggctaagaga 781 tttgatatta agctcccgcc tagatttaga tctcatgatg agcatcttga acaaaaaaga 841 gagatagcat atgtcaatca atgtaagttg aataaaatca ataaaagaaa tgtttttagt 901 cgctgctgga taaatatgaa tctttattct gtaatcaaag ctaaatctgc ggccgcatat 961 actgatattg aaatgaatag attaggtaaa taaggatcca ctagctcgtt tcaaattacc 1021 gatgatatcg gaccgttcca acttaccgac cagttcggca ggtatgtatt tgcgtgcatt 1081 cctatccaaa aaaacatcaa gccaaaagct tgaaaaaact tacaacacag ctcaacagag 1141 ctagattgta aaaccctgct ttgttaagca gaacgcaaaa attgaatgac ttatagtcat 1201 atcgcttcga ccctcgtaga ttagtagcct tgagctatta actggttgaa acacttacca 1261 aataaagatt aaaagcgata aaaatgaaag ataaagcagc taaaaacaga gattttagaa 1321 agactatttt atcagtgtta caacgcaata aagatggctc ttttgctacg caagcaaata 1381 gaaagtctat tctgttgcag gcaactaaag accttaaaaa ggtagggttt agcaaggtta 1441 cagccgaaaa cttcggtaat aagcattgct atgcacttag agaccattgg agagcccaag 1501 gattagctac agcaacgata aaaaatcgtt tagcttgtct aaggtggtta ggcgagaaaa 1561 tgggcaaaga actacccgat aatcgaaaat tagagattga gaacaggaag tatagcgata 1621 attcaatcaa taaagcccaa gaaatcgatt ttaaggcgat ttctgcctta actgataggc 1681 aagccctagc aatacaatta cagcgcgaat ttgggcttcg tagagaagaa agtttgaagt 1741 ttcagcctag ttatgcaatc aaagagcata aaatcgagct taaaagctct tggacaaagg 1801 gtggaagacc acgagaaatc ccaattttga atgaaaaaca gagagaattg ttagaaaaag 1861 taaaagaggt agcaggtaaa ggctctctaa ttgagagcga aaagtcttat aagcaagcaa 1921 tggaacattt cacgactcgc tgtcaaagag cagggattaa gaatgttcat ggctttagac 1981 atgcgtatgc tcaagataga tataggcaat taacagggcg tgagtgtcct aaaaatggtg 2041 gattaacatc taagcagcta acacctgagc aaaagcaaca agactatgaa gctagaatga 2101 ctattagtga agagttaggt catggtagag aagatgtaac agtcaactac ttaggcagat 2161 aaaaagcaat atagctatag aagaaaagaa agctatttta catagtagat cgactcttct 2221 tagggatttt atattttttg ataaatcatc tattttgcta gttaaatcat caaatttatc 2281 atcttgttgt ttgactaaat ctaagaatct attctctttt ttaaaatcgt tcatgcaaac 2341 cgcctatagc tttcttcttt ttctgaaatt atttgtcttc acaccataat taaattccca 2401 tttttataag taaagtcttt taaaagcttg tcagtctctt ctctagaaat gtaccaaatt 2461 ttacctattt taggatactt ttcatgaagc tcttctattt ttccccagtc ctttaatagt 2521 ctacctttag agtctcgtaa atagttatct ttgtgacagg ggcctctttt atctttttta 2581 atgtaactat atgttattcc aacgtcacta ttactattat ccaaatcttt tttagcatgc 2641 cagtaagaac tttcataact taactctatc tttcgacctc tttgatatac aacaataaag 2701 ctatagccag tagtaacaac ctgttttact tttgttaaat ctattaactt cttatttatt 2761 tttttatgtt tttttgaaaa tttaaatatt tctatattca ttcctacact tcctcaaatc 2821 caaatggtag cttatgattc tcttctggtt tcttttctaa tttttttata tttgcaataa 2881 aaactctttt tctatctttg atttttttat tgtcccaatt cctccaagta tcatcacaaa 2941 ccctttcaat atcatgtaaa tgatgatgtc taaatattga tctgacataa tacagatcta 3001 ggtctagttc atcacttaac acaacttctc taagtctttc agatgcttcg attggtatgt 3061 aatcctcttt atttttagta tctaaaagct tttgcttaaa ttcttcttct gtctctgcta 3121 ccttactaac tgtaaacttg atatttgtaa tcttacgacc atgttttctg tgatgatcct 3181 tgtcatcata ggttacaaaa atatccgata attgattaat ctcttctagt gctggtaata 3241 ggaacttatt tttaaaattt gaatatctgt tgctgtaact tttaggtaaa tcaaaatcat 3301 taatcatatc atcgacatac aatacgcaat caactatatt agcataccct gcttgttcgc 3361 ctaatttgct tttgagaagt aagtataatc tgcttgaata cttactttta aatgaaaata 3421 gtaactgtct ttctgcttta gtaaagtact cttgtagttg tatcatgtgt ggcattaatg 3481 accaatgaaa ctcgcaaatt aaagcactgc ttttagggtc tgcttcaata tatgcaaacc 3541 agttagctat cttcgtttgt tctttattca gccatactgg cttagacatt attgagtgca 3601 ttaattgctt caatctcact ctgttatgct taacccctgt agctttttca agatcagata 3661 ggcttatctt atacctgtga aactctttat cttctctttt aaccattgag gcaactaaga 3721 atattaagtt ttgttcttct tttgtaaggc tatactttcc tgcaacaaga gtattagaca 3781 tagctatttc tttgccagca tttacatttt taacttcttt catagaacta gagtcattat 3841 ctcgatatac aaattctata aaacttctat tagtaaaaca actacttcat aaaaaaaagt 3901 agttttaacg atacaaaaag tagttttaaa ttcaaaaagt gatacaaaaa gtagttttaa 3961 attcaaaaag tgatacaaaa agtagtttta aattttttaa aaaagtgctt caaagcctta 4021 tgtagcaata cttacagagg attaaaaaaa aatctgacaa tatataaaga gaatatataa 4081 agagaatatc ttaggggatt ttaaaaaaat cccacagact caaagacttt tttgactttt 4141 taaatcctag aaactatact ttaagtactt atttaagtac atggatttag attatgcaaa 4201 ccgttaatta ttcaactttt agaaatgaac tatctgattc aatggataga gtaacaaaaa 4261 atcatagtcc tatgattgta actagaggtt caaaaaaaga agcagttgtt atgatgtcgt 4321 tagaggattc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 4381 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 4441 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 4501 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 4561 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 4621 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaattc agggcgcaag 4681 ggctgctaaa ggaagcggaa cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg 4741 atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag 4801 gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc 4861 gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac 4921 tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgatcaagag 4981 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc 5041 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 5101 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 5161 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg 5221 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 5281 ttgggcgaag tgccggggca ggatctcctg tcatcccacc ttgctcctgc cgagaaagta 5341 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 5401 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 5461 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 5521 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 5581 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 5641 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 5701 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 5761 atcgccttct atcgccttct tgacgagttc ttctgaattg aaaaaggaag agtatgagta 5821 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 5881 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 5941 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 6001 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg 6061 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 6121 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 6181 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 6241 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 6301 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 6361 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 6421 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 6481 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 6541 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 6601 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 6661 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 6721 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 6781 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 6841 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 6901 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 6961 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc 7021 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 7081 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 7141 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 7201 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 7261 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 7321 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 7381 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 7441 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 7501 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 7561 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag //