LOCUS       Exported                7614 bp ds-DNA     circular SYN 22-JUL-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 22, 2018 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7614
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=pF-PigR-VSVG 2"
     promoter        299..533
                     /note="GroE"
     misc_feature    436..458
                     /label=nonstandard type: PCR_primer
                     /note="AROL2"
     misc_feature    594..625
                     /label=nonstandard type: PCR_primer
                     /note="P346 PigR-VSVG-F"
     primer_bind     600..621
                     /label=P041 INTpEX_FTL0449
                     /note="P041 INTpEX_FTL0449"
     gene            616..948
                     /locus_tag="FTL_0449"
                     /label=FTL_0449
                     /db_xref="GeneID:3952313"
     CDS             616..948
                     /codon_start=1
                     /locus_tag="FTL_0449"
                     /product="hypothetical protein"
                     /label=FTL_0449
                     /note="/dnas_title=PigR"
                     /db_xref="GI:89255859"
                     /db_xref="GeneID:3952313"
                     /inference="ab initio prediction"
                     /protein_id="YP_513221.1"
                     /translation="MANQYSGNFEQIVKNRFKCSAREILLKCQREGLSYQDAEKVLGFK
                     HVTIRKWAKRFDIKLPPRFRSHDEHLEQKREIAYVNQCKLNKINKRNVFSRCWINMNLY
                     SVIKAKS"
     misc_feature    671..690
                     /label=JCOL802 - RT_PCR for PigR
                     /note="JCOL802 - RT_PCR for PigR"
     misc_feature    750..772
                     /label=JCOL803 - RT-PCR for PigR
                     /note="JCOL803 - RT-PCR for PigR"
     misc_feature    complement(918..956)
                     /label=nonstandard type: PCR_primer
                     /note="P056 FTL_0449fragR2"
     gene            complement(924..948)
                     /gene="psd"
                     /locus_tag="FTL_0450"
                     /label=psd
                     /db_xref="GeneID:3952314"
     CDS             complement(924..948)
                     /gene="psd"
                     /locus_tag="FTL_0450"
                     /product="phosphatidylserine decarboxylase"
                     /label=psd
                     /note="catalyzes the decarboxylation of 
                     phosphatidyl-L-serine to phosphatidylethanoleamine"
                     /note="/dnas_title=phosphatidylserine decarboxylase"
                     /db_xref="GI:89255860"
                     /db_xref="GeneID:3952314"
                     /inference="profile:same species:4.1"
                     /protein_id="YP_513222.1"
     CDS             958..990
                     /label=VSV-G
                     /note="VSV-G"
     misc_feature    complement(1119..1141)
                     /label=nonstandard type: PCR_primer
                     /note="AROL3"
     gene            1284..2159
                     /label=ORF3
                     /note="ORF3"
     gene            complement(2382..2801)
                     /label=ORF2
                     /note="ORF2"
     gene            complement(2807..3823)
                     /label=RepA
                     /note="RepA"
     gene            5002..5793
                     /label=kanamycin
                     /note="kanamycin"
     gene            5814..6671
                     /label=ampicillin
                     /note="ampicillin"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccttgta tggattagtc gagctaaaaa gctcatattt tttatattca aactatatac
      301 ccttcaagct ttgaaaaata aacttaatta ttatatatgt tatttagcta gtttttttaa
      361 ttaaagttaa aatcgagagc ttgtttgaca aaaaaacaaa aaaatttctt gaaaattttt
      421 tttttgactc aatatctaga cttgcaagag cttggaactt tgagattgtt ctaagatgca
      481 tacaaattca aaatgcttaa acaaaaataa tttaacaaag gagtaagatt gttatgaaca
      541 ttcgtccatt acaagataga gtattagttc gtcgtgcagg taccatatga attcccggga
      601 aaggagactc gttatatggc gaatcaatat tctggaaatt ttgagcaaat tgtcaaaaat
      661 agatttaaat gctcagcgag agagatctta ttaaaatgcc aaagagaagg cttaagttac
      721 caagatgctg aaaaagtttt gggttttaag catgttacta tcagaaagtg ggctaagaga
      781 tttgatatta agctcccgcc tagatttaga tctcatgatg agcatcttga acaaaaaaga
      841 gagatagcat atgtcaatca atgtaagttg aataaaatca ataaaagaaa tgtttttagt
      901 cgctgctgga taaatatgaa tctttattct gtaatcaaag ctaaatctgc ggccgcatat
      961 actgatattg aaatgaatag attaggtaaa taaggatcca ctagctcgtt tcaaattacc
     1021 gatgatatcg gaccgttcca acttaccgac cagttcggca ggtatgtatt tgcgtgcatt
     1081 cctatccaaa aaaacatcaa gccaaaagct tgaaaaaact tacaacacag ctcaacagag
     1141 ctagattgta aaaccctgct ttgttaagca gaacgcaaaa attgaatgac ttatagtcat
     1201 atcgcttcga ccctcgtaga ttagtagcct tgagctatta actggttgaa acacttacca
     1261 aataaagatt aaaagcgata aaaatgaaag ataaagcagc taaaaacaga gattttagaa
     1321 agactatttt atcagtgtta caacgcaata aagatggctc ttttgctacg caagcaaata
     1381 gaaagtctat tctgttgcag gcaactaaag accttaaaaa ggtagggttt agcaaggtta
     1441 cagccgaaaa cttcggtaat aagcattgct atgcacttag agaccattgg agagcccaag
     1501 gattagctac agcaacgata aaaaatcgtt tagcttgtct aaggtggtta ggcgagaaaa
     1561 tgggcaaaga actacccgat aatcgaaaat tagagattga gaacaggaag tatagcgata
     1621 attcaatcaa taaagcccaa gaaatcgatt ttaaggcgat ttctgcctta actgataggc
     1681 aagccctagc aatacaatta cagcgcgaat ttgggcttcg tagagaagaa agtttgaagt
     1741 ttcagcctag ttatgcaatc aaagagcata aaatcgagct taaaagctct tggacaaagg
     1801 gtggaagacc acgagaaatc ccaattttga atgaaaaaca gagagaattg ttagaaaaag
     1861 taaaagaggt agcaggtaaa ggctctctaa ttgagagcga aaagtcttat aagcaagcaa
     1921 tggaacattt cacgactcgc tgtcaaagag cagggattaa gaatgttcat ggctttagac
     1981 atgcgtatgc tcaagataga tataggcaat taacagggcg tgagtgtcct aaaaatggtg
     2041 gattaacatc taagcagcta acacctgagc aaaagcaaca agactatgaa gctagaatga
     2101 ctattagtga agagttaggt catggtagag aagatgtaac agtcaactac ttaggcagat
     2161 aaaaagcaat atagctatag aagaaaagaa agctatttta catagtagat cgactcttct
     2221 tagggatttt atattttttg ataaatcatc tattttgcta gttaaatcat caaatttatc
     2281 atcttgttgt ttgactaaat ctaagaatct attctctttt ttaaaatcgt tcatgcaaac
     2341 cgcctatagc tttcttcttt ttctgaaatt atttgtcttc acaccataat taaattccca
     2401 tttttataag taaagtcttt taaaagcttg tcagtctctt ctctagaaat gtaccaaatt
     2461 ttacctattt taggatactt ttcatgaagc tcttctattt ttccccagtc ctttaatagt
     2521 ctacctttag agtctcgtaa atagttatct ttgtgacagg ggcctctttt atctttttta
     2581 atgtaactat atgttattcc aacgtcacta ttactattat ccaaatcttt tttagcatgc
     2641 cagtaagaac tttcataact taactctatc tttcgacctc tttgatatac aacaataaag
     2701 ctatagccag tagtaacaac ctgttttact tttgttaaat ctattaactt cttatttatt
     2761 tttttatgtt tttttgaaaa tttaaatatt tctatattca ttcctacact tcctcaaatc
     2821 caaatggtag cttatgattc tcttctggtt tcttttctaa tttttttata tttgcaataa
     2881 aaactctttt tctatctttg atttttttat tgtcccaatt cctccaagta tcatcacaaa
     2941 ccctttcaat atcatgtaaa tgatgatgtc taaatattga tctgacataa tacagatcta
     3001 ggtctagttc atcacttaac acaacttctc taagtctttc agatgcttcg attggtatgt
     3061 aatcctcttt atttttagta tctaaaagct tttgcttaaa ttcttcttct gtctctgcta
     3121 ccttactaac tgtaaacttg atatttgtaa tcttacgacc atgttttctg tgatgatcct
     3181 tgtcatcata ggttacaaaa atatccgata attgattaat ctcttctagt gctggtaata
     3241 ggaacttatt tttaaaattt gaatatctgt tgctgtaact tttaggtaaa tcaaaatcat
     3301 taatcatatc atcgacatac aatacgcaat caactatatt agcataccct gcttgttcgc
     3361 ctaatttgct tttgagaagt aagtataatc tgcttgaata cttactttta aatgaaaata
     3421 gtaactgtct ttctgcttta gtaaagtact cttgtagttg tatcatgtgt ggcattaatg
     3481 accaatgaaa ctcgcaaatt aaagcactgc ttttagggtc tgcttcaata tatgcaaacc
     3541 agttagctat cttcgtttgt tctttattca gccatactgg cttagacatt attgagtgca
     3601 ttaattgctt caatctcact ctgttatgct taacccctgt agctttttca agatcagata
     3661 ggcttatctt atacctgtga aactctttat cttctctttt aaccattgag gcaactaaga
     3721 atattaagtt ttgttcttct tttgtaaggc tatactttcc tgcaacaaga gtattagaca
     3781 tagctatttc tttgccagca tttacatttt taacttcttt catagaacta gagtcattat
     3841 ctcgatatac aaattctata aaacttctat tagtaaaaca actacttcat aaaaaaaagt
     3901 agttttaacg atacaaaaag tagttttaaa ttcaaaaagt gatacaaaaa gtagttttaa
     3961 attcaaaaag tgatacaaaa agtagtttta aattttttaa aaaagtgctt caaagcctta
     4021 tgtagcaata cttacagagg attaaaaaaa aatctgacaa tatataaaga gaatatataa
     4081 agagaatatc ttaggggatt ttaaaaaaat cccacagact caaagacttt tttgactttt
     4141 taaatcctag aaactatact ttaagtactt atttaagtac atggatttag attatgcaaa
     4201 ccgttaatta ttcaactttt agaaatgaac tatctgattc aatggataga gtaacaaaaa
     4261 atcatagtcc tatgattgta actagaggtt caaaaaaaga agcagttgtt atgatgtcgt
     4321 tagaggattc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4381 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4441 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4501 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4561 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4621 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaattc agggcgcaag
     4681 ggctgctaaa ggaagcggaa cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg
     4741 atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag
     4801 gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc
     4861 gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac
     4921 tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgatcaagag
     4981 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     5041 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     5101 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     5161 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     5221 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     5281 ttgggcgaag tgccggggca ggatctcctg tcatcccacc ttgctcctgc cgagaaagta
     5341 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     5401 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     5461 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     5521 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     5581 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     5641 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     5701 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     5761 atcgccttct atcgccttct tgacgagttc ttctgaattg aaaaaggaag agtatgagta
     5821 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5881 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5941 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6001 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     6061 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     6121 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     6181 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     6241 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     6301 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     6361 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     6421 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     6481 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     6541 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     6601 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     6661 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     6721 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     6781 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6841 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6901 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6961 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     7021 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7081 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7141 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7201 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7261 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7321 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7381 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7441 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7501 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     7561 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag
//
