LOCUS       pF-PmrA-VSVG            7965 bp    DNA     circular     21-JUL-2018
FEATURES             Location/Qualifiers
     promoter        299..533
                     /note="GroE"
     gene            1635..2510
                     /note="ORF3"
     gene            complement(2733..3152)
                     /note="ORF2"
     gene            complement(3158..4174)
                     /note="RepA"
     gene            5353..6144
                     /note="kanamycin"
     gene            6165..7022
                     /note="ampicillin"
     PCR_primer      436..458
                     /note="AROL2"
     PCR_primer      complement(1470..1492)
                     /note="AROL3"
     CDS             1309..1341
                     /note="VSV-G"
     primer_bind     773..799
                     /note="P018 FTL_0552fragF"
     primer_bind     686..713
                     /note="P044 INTpEX_FTL0552"
     primer_bind     616..642
                     /note="P238 FTL_0552F"
     primer_bind     600..627
                     /note="P301 PmrA_comp_F"
     primer_bind     complement(1272..1299)
                     /note="P017 FTL_0552fragR"
     gene            616..1299
                     /locus_tag="FTL_0552"
                     /db_xref="GeneID:3952730"
     CDS             616..1299
                     /dnas_title="two-component response regulator"
                     /locus_tag="FTL_0552"
                     /inference="profile:Pfam:18"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator"
                     /protein_id="YP_513318.1"
                     /db_xref="GI:89255956"
                     /db_xref="GeneID:3952730"
                     /translation="MRILLAEDDLHLGEGLLEALQKEGLIVNLVSDGEAAQTFIESGL
                     YDIVVLDIGMPIKTGLEVLRNIRNRGIKVPIILLTARDGLEDRIKGLDLGADDYLTKP
                     FELKELVARIKAISRRIDTRSGKSVNEEIRFGDYSFNPSSETVTKDGVIIPVSKKELA
                     LLAILVQNAGRVVPKTQLLEEIYSTDKEMDTNTLEVHMHNLRKKINIPNFIQTIRGVG
                     YFVQKDKVIK"
     primer_bind     complement(600..624)
                     /note="P229 PmrADel5R"
     promoter        5215..5264
                     /note="pCR2.1 NEOKAN promoter"
     source          1..7965
                     /dnas_title="pF-PmrA-VSVG"
ORIGIN      
        1 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC
       61 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC
      121 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA
      181 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTTG
      241 GTACcttgta tggattagtc gagctaaaaa gctcatattt tttatattca aactatatac
      301 ccttcaagct ttgaaaaata aacttaatta ttatatatgt tatttagcta gtttttttaa
      361 ttaaagttaa aatcgagagc ttgtttgaca aaaaaacaaa aaaatttctt gaaaattttt
      421 tttttgactc aatatctaga cttgcaagag cttggaactt tgagattgtt ctaagatgca
      481 tacaaattca aaatgcttaa acaaaaataa tttaacaaag gagtaagatt gttatgaaca
      541 ttcgtccatt acaagataga gtattagttc gtcgtgcagg tacCatatga attcccggga
      601 tagcaagggt taattatgag aatattgttg gctgaagatg atcttcattt gggtgaaggc
      661 ttattagaag ctttgcaaaa agagggtttg attgtaaatc tagtttcaga tggtgaggca
      721 gcgcaaactt ttatagaatc tggattgtat gatatagtcg tcttagatat tggtatgcca
      781 ataaaaactg gtttagaagt tctaagaaat attagaaata gaggtattaa agtaccaata
      841 atactgttga ctgctagaga tggtttagaa gatagaatta aaggtcttga tcttggtgct
      901 gatgattatc taactaagcc atttgagtta aaagagctag tagctagaat taaagctatt
      961 tctcgtcgta ttgatactag atctggtaaa agtgttaatg aagaaattag atttggagat
     1021 tatagtttta atccaagctc tgaaacggta accaaagatg gagttattat tcctgtttca
     1081 aaaaaagagt tagcgctttt agctatattg gtacaaaatg ctggtagggt agtaccaaag
     1141 acacagcttt tagaagaaat atattctact gataaggaaa tggatacaaa caccctagaa
     1201 gtacatatgc ataatttaag aaagaaaatt aacattccta attttataca gactattaga
     1261 ggtgttggtt actttgtaca aaaggataaa gtaattaagG CGGCCGCaTA TACTGATATT
     1321 GAAATGAATA GATTAGGTAA ATAAGGATCC ACTAGCTCGT TTCAAATTAC CGATGATATC
     1381 GGACCGTTCC AACTTACCGA CCAGTTCGGC AGGTATGTAT TTGCGTGCAT TCCTATCCAA
     1441 AAAAACATCA AGCCAAAAGC TTGAAAAAAC TTACAACACA GCTCAACAGA GCTAGATTGT
     1501 AAAACCCTGC TTTGTTAAGC AGAACGCAAA AATTGAATGA CTTATAGTCA TATCGCTTCG
     1561 ACCCTCGTAG ATTAGTAGCC TTGAGCTATT AACTGGTTGA AACACTTACC AAATAAAGAT
     1621 TAAAAGCGAT AAAAATGAAA GATAAAGCAG CTAAAAACAG AGATTTTAGA AAGACTATTT
     1681 TATCAGTGTT ACAACGCAAT AAAGATGGCT CTTTTGCTAC GCAAGCAAAT AGAAAGTCTA
     1741 TTCTGTTGCA GGCAACTAAA GACCTTAAAA AGGTAGGGTT TAGCAAGGTT ACAGCCGAAA
     1801 ACTTCGGTAA TAAGCATTGC TATGCACTTA GAGACCATTG GAGAGCCCAA GGATTAGCTA
     1861 CAGCAACGAT AAAAAATCGT TTAGCTTGTC TAAGGTGGTT AGGCGAGAAA ATGGGCAAAG
     1921 AACTACCCGA TAATCGAAAA TTAGAGATTG AGAACAGGAA GTATAGCGAT AATTCAATCA
     1981 ATAAAGCCCA AGAAATCGAT TTTAAGGCGA TTTCTGCCTT AACTGATAGG CAAGCCCTAG
     2041 CAATACAATT ACAGCGCGAA TTTGGGCTTC GTAGAGAAGA AAGTTTGAAG TTTCAGCCTA
     2101 GTTATGCAAT CAAAGAGCAT AAAATCGAGC TTAAAAGCTC TTGGACAAAG GGTGGAAGAC
     2161 CACGAGAAAT CCCAATTTTG AATGAAAAAC AGAGAGAATT GTTAGAAAAA GTAAAAGAGG
     2221 TAGCAGGTAA AGGCTCTCTA ATTGAGAGCG AAAAGTCTTA TAAGCAAGCA ATGGAACATT
     2281 TCACGACTCG CTGTCAAAGA GCAGGGATTA AGAATGTTCA TGGCTTTAGA CATGCGTATG
     2341 CTCAAGATAG ATATAGGCAA TTAACAGGGC GTGAGTGTCC TAAAAATGGT GGATTAACAT
     2401 CTAAGCAGCT AACACCTGAG CAAAAGCAAC AAGACTATGA AGCTAGAATG ACTATTAGTG
     2461 AAGAGTTAGG TCATGGTAGA GAAGATGTAA CAGTCAACTA CTTAGGCAGA TAAAAAGCAA
     2521 TATAGCTATA GAAGAAAAGA AAGCTATTTT ACATAGTAGA TCGACTCTTC TTAGGGATTT
     2581 TATATTTTTT GATAAATCAT CTATTTTGCT AGTTAAATCA TCAAATTTAT CATCTTGTTG
     2641 TTTGACTAAA TCTAAGAATC TATTCTCTTT TTTAAAATCG TTCATGCAAA CCGCCTATAG
     2701 CTTTCTTCTT TTTCTGAAAT TATTTGTCTT CACACCATAA TTAAATTCCC ATTTTTATAA
     2761 GTAAAGTCTT TTAAAAGCTT GTCAGTCTCT TCTCTAGAAA TGTACCAAAT TTTACCTATT
     2821 TTAGGATACT TTTCATGAAG CTCTTCTATT TTTCCCCAGT CCTTTAATAG TCTACCTTTA
     2881 GAGTCTCGTA AATAGTTATC TTTGTGACAG GGGCCTCTTT TATCTTTTTT AATGTAACTA
     2941 TATGTTATTC CAACGTCACT ATTACTATTA TCCAAATCTT TTTTAGCATG CCAGTAAGAA
     3001 CTTTCATAAC TTAACTCTAT CTTTCGACCT CTTTGATATA CAACAATAAA GCTATAGCCA
     3061 GTAGTAACAA CCTGTTTTAC TTTTGTTAAA TCTATTAACT TCTTATTTAT TTTTTTATGT
     3121 TTTTTTGAAA ATTTAAATAT TTCTATATTC ATTCCTACAC TTCCTCAAAT CCAAATGGTA
     3181 GCTTATGATT CTCTTCTGGT TTCTTTTCTA ATTTTTTTAT ATTTGCAATA AAAACTCTTT
     3241 TTCTATCTTT GATTTTTTTA TTGTCCCAAT TCCTCCAAGT ATCATCACAA ACCCTTTCAA
     3301 TATCATGTAA ATGATGATGT CTAAATATTG ATCTGACATA ATACAGATCT AGGTCTAGTT
     3361 CATCACTTAA CACAACTTCT CTAAGTCTTT CAGATGCTTC GATTGGTATG TAATCCTCTT
     3421 TATTTTTAGT ATCTAAAAGC TTTTGCTTAA ATTCTTCTTC TGTCTCTGCT ACCTTACTAA
     3481 CTGTAAACTT GATATTTGTA ATCTTACGAC CATGTTTTCT GTGATGATCC TTGTCATCAT
     3541 AGGTTACAAA AATATCCGAT AATTGATTAA TCTCTTCTAG TGCTGGTAAT AGGAACTTAT
     3601 TTTTAAAATT TGAATATCTG TTGCTGTAAC TTTTAGGTAA ATCAAAATCA TTAATCATAT
     3661 CATCGACATA CAATACGCAA TCAACTATAT TAGCATACCC TGCTTGTTCG CCTAATTTGC
     3721 TTTTGAGAAG TAAGTATAAT CTGCTTGAAT ACTTACTTTT AAATGAAAAT AGTAACTGTC
     3781 TTTCTGCTTT AGTAAAGTAC TCTTGTAGTT GTATCATGTG TGGCATTAAT GACCAATGAA
     3841 ACTCGCAAAT TAAAGCACTG CTTTTAGGGT CTGCTTCAAT ATATGCAAAC CAGTTAGCTA
     3901 TCTTCGTTTG TTCTTTATTC AGCCATACTG GCTTAGACAT TATTGAGTGC ATTAATTGCT
     3961 TCAATCTCAC TCTGTTATGC TTAACCCCTG TAGCTTTTTC AAGATCAGAT AGGCTTATCT
     4021 TATACCTGTG AAACTCTTTA TCTTCTCTTT TAACCATTGA GGCAACTAAG AATATTAAGT
     4081 TTTGTTCTTC TTTTGTAAGG CTATACTTTC CTGCAACAAG AGTATTAGAC ATAGCTATTT
     4141 CTTTGCCAGC ATTTACATTT TTAACTTCTT TCATAGAACT AGAGTCATTA TCTCGATATA
     4201 CAAATTCTAT AAAACTTCTA TTAGTAAAAC AACTACTTCA TAAAAAAAAG TAGTTTTAAC
     4261 GATACAAAAA GTAGTTTTAA ATTCAAAAAG TGATACAAAA AGTAGTTTTA AATTCAAAAA
     4321 GTGATACAAA AAGTAGTTTT AAATTTTTTA AAAAAGTGCT TCAAAGCCTT ATGTAGCAAT
     4381 ACTTACAGAG GATTAAAAAA AAATCTGACA ATATATAAAG AGAATATATA AAGAGAATAT
     4441 CTTAGGGGAT TTTAAAAAAA TCCCACAGAC TCAAAGACTT TTTTGACTTT TTAAATCCTA
     4501 GAAACTATAC TTTAAGTACT TATTTAAGTA CATGGATTTA GATTATGCAA ACCGTTAATT
     4561 ATTCAACTTT TAGAAATGAA CTATCTGATT CAATGGATAG AGTAACAAAA AATCATAGTC
     4621 CTATGATTGT AACTAGAGGT TCAAAAAAAG AAGCAGTTGT TATGATGTCG TTAGAGGATT
     4681 CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT
     4741 CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG
     4801 TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA
     4861 GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC
     4921 GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA
     4981 GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATT CAGGGCGCAA GGGCTGCTAA
     5041 AGGAAGCGGA ACACGTAGAA AGCCAGTCCG CAGAAACGGT GCTGACCCCG GATGAATGTC
     5101 AGCTACTGGG CTATCTGGAC AAGGGAAAAC GCAAGCGCAA AGAGAAAGCA GGTAGCTTGC
     5161 AGTGGGCTTA CATGGCGATA GCTAGACTGG GCGGTTTTAT GGACAGCAAG CGAACCGGAA
     5221 TTGCCAGCTG GGGCGCCCTC TGGTAAGGTT GGGAAGCCCT GCAAAGTAAA CTGGATGGCT
     5281 TTCTTGCCGC CAAGGATCTG ATGGCGCAGG GGATCAAGAT CTGATCAAGA GACAGGATGA
     5341 GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG
     5401 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG
     5461 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC
     5521 CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT
     5581 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA
     5641 GTGCCGGGGC AGGATCTCCT GTCATCCCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG
     5701 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA
     5761 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT
     5821 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG
     5881 CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC
     5941 ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC
     6001 CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG
     6061 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC
     6121 TATCGCCTTC TTGACGAGTT CTTCTGAATT GAAAAAGGAA GAGTATGAGT ATTCAACATT
     6181 TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG
     6241 AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG
     6301 AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA
     6361 TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC
     6421 AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG
     6481 TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA
     6541 CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC
     6601 TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG
     6661 AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA
     6721 CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA
     6781 TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG
     6841 GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG
     6901 CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG
     6961 CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT
     7021 GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT
     7081 AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC
     7141 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG
     7201 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG
     7261 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA
     7321 GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA
     7381 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA
     7441 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC
     7501 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA
     7561 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA
     7621 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC
     7681 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC
     7741 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG
     7801 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT
     7861 CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA
     7921 GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAG
//

