	   SnapGene       TATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAATCGATCAAGGGTTGGTTTGCGCATTCACAGTTCTCCGCAAGAATTGATTGGCTCCAATTCTTGGAGTGGTGAATCCGTTAGCGAGGTGCCGCCGGCTTCCATTCAGGTCGAGGTGGCCCGGCTCCATGCACCGCGACGCAACGCGGGGAGGCAGACAAGGTATAGGGCGGCGCCTACAATCCATGCCAACCCGTTCCATGTGCTCGCCGAGGCGGCATAAATCGCCGTGACGATCAGCGGTCCAATGATCGAAGTTAGGCTGGTAAGAGCCGCGAGCGATCCTTGAAGCTGTCCCTGATGGTCGTCATCTACCTGCCTGGACAGCATGGCCTGCAACGCGGGCATCCCGATGCCGCCGGAAGCGAGAAGAATCATAATGGGGAAGGCCATCCAGCCTCGCGTCGATCTATACCCTTCAAGCTTTGAAAAATAAACTTAATTATTATATATGTTATTTAGCTAGTTTTTTTAATTAAAGTTAAAATCGAGAGCTTGTTTGACAAAAAAACAAAAAAATTTCTTGAAAATTTTTTTTTTGACTCAATATCTAGACTTGCAAGAGCTTGGAACTTTGAGATTGTTCTAAGATGCATACAAATTCAAAATGCTTAAACAAAAATAATTTAACAAAGGAGTAAGATTGTTATGAGCCATATTCAACGGGAAACGTCTTGCTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGATCACGCATCTTCCCGACAACGCAGACCGTTCCGTGGCAAAGCAAAAGTTCAAAATCACCAACTGGTCCACCTACAACAAAGCTCTCATCAACGATATCCCGCAAGAGGCCCGGCAGTACCGGCATAACCAAGCCTATGCCTACAGCATCCAGGGTGACGGTGCCGAGGATGACGATGAGCGCATTGTTAGATTTCATACACGGTGCCTGACTGCGTTAGCAATTTAACTGTGATAAACTACCGCATTAAAGCTAATCGGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAGAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATAATCGGCATTTTCTTTTGCGTTTTTATTTGTTAACTGTTAATTGTCCTTGTTCAAGGATGCTGTCTTTGACAACAGATGTTTTCTTGCCTTTGATGTTCAGCAGGAAGCTAGGCGCAAACGTTGATTGTTTGTCTGCGTAGAATCCTCTGTTTGTCATATAGCTTGTAATCACGACATTGTTTCCTTTCGCTTGAGGTACAGCGAAGTGTGAGTAAGTAAAGGTTACATCGTTAGGATCAAGATCCATTTTTAACACAAGGCCAGTTTTGTTCAGCGGCTTGTATGGGCCAGTTAAAGAATTAGAAACATAACCAAGCATGTAAATATCGTTAGACGTAATGCCGTCAATCGTCATTTTTGATCCGCGGGAGTCAGTGAACAGATACCATTTGCCGTTCATTTTAAAGACGTTCGCGCGTTCAATTTCATCTGTTACTGTGTTAGATGCAATCAGCGGTTTCATCACTTTTTTCAGTGTGTAATCATCGTTTAGCTCAATCATACCGAGAGCGCCGTTTGCTAACTCAGCCGTGCGTTTTTTATCGCTTTGCAGAAGTTTTTGACTTTCTTGACGGAAGAATGATGTGCTTTTGCCATAGTATGCTTTGTTAAATAAAGATTCTTCGCCTTGGTAGCCATCTTCAGTTCCAGTGTTTGCTTCAAATACTAAGTATTTGTGGCCTTTATCTTCTACGTAGTGAGGATCTCTCAGCGTATGGTTGTCGCCTGAGCTGTAGTTGCCTTCATCGATGAACTGCTGTACATTTTGATACGTTTTTCCGTCACCGTCAAAGATTGATTTATAATCCTCTACACCGTTGATGTTCAAAGAGCTGTCTGATGCTGATACGTTAACTTGTGCAGTTGTCAGTGTTTGTTTGCCGTAATGTTTACCGGAGAAATCAGTGTAGAATAAACGGATTTTTCCGTCAGATGTAAATGTGGCTGAACCTGACCATTCTTGTGTTTGGTCTTTTAGGATAGAATCATTTGCATCGAATTTGTCGCTGTCTTTAAAGACGCGGCCAGCGTTTTTCCAGCTGTCAATAGAAGTTTCGCCGACTTTTTGATAGAACATGTAAATCGATGTGTCATCCGCATTTTTAGGATCTCCGGCTAATGCAAAGACGATGTGGTAGCCGTGATAGTTTGCGACAGTGCCGTCAGCGTTTTGTAATGGCCAGCTGTCCCAAACGTCCAGGCCTTTTGCAGAAGAGATATTTTTAATTGTGGACGAATCGAACTCAGGAACTTGATATTTTTCATTTTTTTGCTGTTCAGGGATTTGCAGCATATCATGGCGTGTAATATGGGAAATGCCGTATGTTTCCTTATATGGCTTTTGGTTCGTTTCTTTCGCAAACGCTTGAGTTGCGCCTCCTGCCAGCAGTGCGGTAGTAAAGGTTAATACTGTTGCTTGTTTTGCAAACTTTTTGATGTTCATCGTTCATGTCTCCTTTTTTATGTACTGTGTTAGCGGTCTGCTTCTTCCAGCCCTCCTGTTTGAAGATGGCAAGTTAGTTACGCACAATAAAAAAAGACCTAAAATATGTAAGGGGTGACGCCAAAGTATACACTTTGCCCTTTACACATTTTAGGTCTTGCCTGCTTTATCAGTAACAAACCCGCGCGATTTACTTTTCGACCTCATTCTATTAGACTCTCGTTTGGATTGCAACTGGTCTATTTTCCTCTTTTGTTTGATAGAAAATCATAAAAGGATTTGCAGACTACGGGCCTAAAGAACTAAAAAATCTATCTGTTTCTTTTCATTCTCTGTATTTTTTATAGTTTCTGTTGCATGGGCATAAAGTTGCCTTTTTAATCACAATTCAGAAAATATCATAATATCTCATTTCACTAAATAATAGTGAACGGCAGGTATATGTGATGGGTTAAAAAGGATCGATCCTCTAGCTAGAGTCGATCTTCGCCAGCAGGGCGAGGATCGTGGCATCACCGAACCGCGCCGTGCGCGGGTCGTCGGTGAGCCAGAGTTTCAGCAGGCCGCCCAGGCGGCCCAGGTCGCCATTGATGCGGGCCAGCTCGCGGACGTGCTCATAGTCCACGACGCCCGTGATTTTGTAGCCCTGGCCGACGGCCAGCAGGTAGGCCGACAGGCTCATGCCGGCTGCCGCCGCCTTTTCCTCAATCGCTCTTCGTTCGTCTGGAAGGCAGTACACCTTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGCTGCTTCCCTGCTGTTTTGTGGAATATCTACCGACTGGAAACAGGCAAATGCAGGAAATTACTGAACTGAGGGGACAGGCGAGAGACGATGCCAAAGAGCTACACCGACGAGCTGGCCGAGTGGGTTGAATCCCGCGCGGCCAAGAAGCGCCGGCGTGATGAGGCTGCGGTTGCGTTCCTGGCGGTGAGGGCGGATGTCGATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCATATTTGAATGTATTTAGAAAAATAAACAAAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACAGCCAAGCTCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCcaacttgtcatatcaaatttaatgaagctactgcaatcttagcgggcgatgctttacaagcattagcttttgaaatattacaagatatcaactactcagacatagataaactaaaaaaaataaataaactttttgctcaatactgtggtgctagtggtatggttggtggtcaacaactagatattgagggtgagaataaaaaacttaaaattgaagaattacaaatattacatatcaataaaactgcaaaaatgtttagagcatgtatagttttaccttatattctctcacaaaatcaaaatagccaaatagaagatttattagtaaggttatcagacttaataggcttatgttttcagatcaaagatgatatcttagatgtcactaagacaactaacgagctaggaaaaactagcgctaaagatatagatgccaataaatcaacctatgtatcattaatgggattaaaagacgcaaataaatatttactagataaaaaaaatgaaatcacacaaattatcactaagctaaaaaataataatttcagttcaactagtcttgaaaatctcattgatttagttattaatagaaactgttagttatttttgctacaattccgtaaatttatttaaaagttaatattaaaatgcaagtaGCGGCCGCTaaaagttaatctgtttacctcttaatcacttctatatgataatatattaaataagttaagctgtttttataattctatatgaaagttttaagtcaagaagagcgccaaaaactctttttagaaaatatttttccatataaacataagattcctcgtaatgtatatgaaaaacaaaagcattatcttcagatagaactattaaaatttcagaaatgggtaaaagagaataataaaaaagttctgataatttttgaaggtagagatgcagctggtaagggtggaactataaaaagaatgatggagcatctaaatccacgtggcgccaaggtaattgctctagagaaaccatcagaacaagagagaaatcaatggtattttcaacgatacatagaacacctaccatctggtggtgaaatcgtactttttgataggtcttggtataatcgagctggtgttgaaagagttatgggcttttgtactgagagagaatatttcttattccttgaacaagcaccccaactagaaaaaatgctagttgatagtggtactatgataataaaattttggttctcagttagccagcaagaacaaaaaaatagatttgctgctagagaaagtcatcccttaaaacagtggaaacttagccctatagataaagcatccttagataaatgggatgactatactgaagctaaagaaaggatgtttatatatGGTACCGAGCTCGAATTCGTAATCATGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACCCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAAC  F/                  "   $   	   $   !         %         $            !   $   8          4   (   $                                  %   !      &   )   >   #               )         $   &   "   '      -      $   #   &   1   "               $   $   6   (            .         #   (      $   2      "   ;   |   N   -   "   ;   *   6      $   )      $       $   '      9   3         %   B      !   4       #                  '                                       *   #      +   4         #   9      "   !   "   +      &            K   4      !   "   ,   &                  ;   .   -      3   +      I   2   y   7      /   %   '               4   &   *   "         )            #               "                     +            %   %   )   %       (       3               '                      !   (   #               $      2         '                  '   #            #      "                        7ZDU	C
afg \[E_
yb4h 8	V	2
<
22I n&
D
?o
9FF- \A	
0c"  H/F`2f
X>[k|5oP$ @ ]x.	

b6Kz0di/}	 H{2$ Z p  9		3
=
:zAm5!!  9 O    h@;KvJr'=k   j).	W	U@F!3{"! 9d3'	B?	3`!V% W or	'
9

WHBw:_v.d}$C ,  _
W`V
|
`rL ~ 'A+P/E
I
(%3G^$ s <JqTR	{x)@NZpR|"Gl M 7]oP	94 j5E		k
r71dQvSUW 	 c  	B7sm
JCg?! I QG*	
]
tQ=_o 3i0Cg$ k    07G	
C4Z,6o"Q8  Mragk*G	k			#?l}3Xpb"p'D;}+k'  :3"T:	P^L\MSYI  I \	h,QYH"3k4 &`c2Aip	



4e]j	'Ej
HTx0( FOD/	yL9_.CK/e<~$ A ^l	Hw;b

{1ey  #+y]zci7=A  >P/Dy			sI|  c			

%=_.  "9g/*3 D2|&fHm n q  F:&FYkt	" 9		H2;i 4
>
F.SU<76g [ p	o
,{Bmn\ T   /VLmgs%  : DK
LPW ,EKO ! P   iA>		BkQw((l X[f
<p2N>&  /Y6

GY}l1|8)  WLk	

V^(09ei@4#-1UwAq>   *`u	


+A`{~>	VZwE"Ti<:3`"F# e X	

tP`Zh 4Z@
Jd47aW e	"
,
WW  :f	b#	|		


Cj$@
("  (
$) X  Xp,		
0
:
T
>v=0XmyI<Djbi~ K N3ux5A} ;`we% X I		*


@}*&D$  
s]		(

v'.DCx)y/P^ & - 0 F  `]X	
_

16	K\a/9yM{"   SL
7	asu+rc' Mfpax	W
}
Y<E]# I_	q
nq"q-   ,QF

Sm)gqFQ&\o~2]M_  ]  
zPpk{:p%$ (m(0	J
5	 D2I4H9v#   ABkl8	euaEx1ARrT`& !$),r
 x )OEOms1 & 3  KS|r	|y?dq+Sv}X
" t uS;Ukq
 M\*AW5HH  =S	
NR[6# [m~	T	%
x18e ? w@

a5JW/c{/n.1 N 28g:T_y!q  $ V ^IpQ		
z(MVqA{!$u2|B$ H b  OD	\
l
s6B^fPu>TV6   69j)F		O3"G2Wo&2CRX|*( "kGa

h38\&-B@GS.w;  b!	
r{8e<)^ m E
8o	n
ER1s  CZKOf. d   _tXdhvDhO~0Tv_@ 
 a' J  2 H+	
/
@=u?|<;Cxih# / E   Lw	
^

J`p."p(  l7		
dRt[j}1$D\qS 2  RRj
M>*NV4	$ ZvfH?
Sy..z 7dt p-{2    !8FiN	gq5[zFe,F]O=): l `n	DNc}Seu^"   1kK6i	


-B!o#R;  u>h5m	Rpy-EAdycE~Rt N]<(,z9|   Nstst#0189l				uvwx$,MNO@4YZcwxKLMNOqrQ^s;MZ[\~N u$2{l:XH	P		"%Pu~5[#PTG_<El-   Oy,b&t+L			
 m_qFXs((" A%	Mm;	##vN`F; v3#;U	I	Q		r]6\$QUe/#XTWZ* 6|4Y
_B?H=hJm~6 {;I}x
9YiQM&Q z,Q JU\c&,A
!rf$ <   -;]2M	
tn`	l) x Pz'u
#N

iRZI-4GYbt)4x   e			-#)$ BhN>D^
_yGD;  hO
a BRq(			f$I$ R <	

$"0	Ok8U1U' G'&9d3njo	
R

5k7w(Fay kR		M t@0$0Z9 ?w4$rGVcJ		v
	s*!]DRVUXf*73 V<PE	4	:`^G%/LY[79 b0	mz7f= 
ICZ	 	c


(C gn*% B mx$				Sz' [%8B  _75O<e	6	@	c	

ntU`<bi|2mISA4IM>i] .X}6df @dK!  # y
[]b[Nt>-	4 p<H~>			Y
 d:FZjR''>1&BRn   ,z^$&8RV<`N{(#  $|!NT{JGzj. t  | 5]0'-		=}  KEB	#Jg  Udz	
-|"} ?QVtlCsV = `  
'u	
>o$
/'  dy.<^03N	

wpa*`=m|	 2	a			&Yr>   	ul ) 6 (u$h

+j4h0.h*PE y  #O
:Jn5HZcu5y4  {vI[Z+ QZnSW8v  K ]o$sd9@z  Er f	s
3q?   ,u			.}  d'gsI*n C	`3HE, GeGZjO* 8 o     ci-?&EA luz~
&4<# + r ;*OS	8
H
'_K^#  iPr)	j
8I3P<n 2R+ j  q![S	9		g+YL!
n:j!j4  *_b1:@ENh	

x^iDJd =.Cx				
1%G G-T%;E6Xljfh# S =	
e
VUo1jPlM9  KX5






*'/=s8UY|?S09,~72_pg "	 
?



Ici#
VV"  w4S


-l|)(C%Oa] !  Heo\	p
6X058xqbz" \  'jk
~lpCHPn`o9+  (:{pq	

[5Gr U lS		
`I1m&  C	2N!VnAR%1t{ 0Wgur/ D   c;Mwh[  YE	
M-|\K x+z%sWK		Q
!ot+DL^EJSW}FP]r:LY '84 @~5LH|w

XA"."WyVg+P ; w :%d	M

se ! Q N8=QnQ
C/6x7C:" ja	s )686,  WB	
		5	?		
;alHR@R&3LZ7\c)&   "  oSF			%U_0%M'sm  { 4,{k<B|U _  x&/t1	=Xq)< Ymg
*3V7gO  c\nc8p ?>y   b,7
@2}%+;  i  )p0?ZDg	8	


7w]XhmKC 	m9i.  J!		d





)H:bDWkLo-  iz[

}ZW/o~4~4_8$ C B	_1bH:fUQ.
3 X}*9y	L


r
n@{-!OV\x9+ ! nM7R						
$.r5?$62K&Bb5r
   l%W)jAT(xI    (ao+>yf7	




|v?Va.n}W3VJjB^7lK#2 86Q	A	
q	a#Gm?-qT>JN4y     /`P=x23vw 	




opKLMuF
-=>cdjklm*+,_nopU=\];56jkJ7 04ax	d			

,1 BNe[`xF#+8<?^aG  		2,9ML[/ }/h Y!5b	

!CO_fuaG$!=+@% f 1y

)7F\eya_' *~@Y	e	-IgKQAeb5;Hi  	A
z
8u?
T\ 5[mnc\O-.G  	\^5:MXZ $ V	3KebwZ[N?4 C0	#
-
Z

;P=UvH(?":'>,Ao& qi!ZI6?	
eg2cCx* f="c<&		"D[k`Sg (b%:SX" S~p		!GXzCt   ;c	


%AWhz=|b 2_z}	*Sa)  g  -{g>


'8DkG] )KO)#`3 
:$		9;%fYb  AUZ	/Bu6j   +D}O){k%J# %\K
.'Rfc<d "|Hf		hLRIV 6Y^
1
;
B
>1nE  .1-		

z=|  Y  (			?>9J@ Uj " }  &@q.V_U
{
wYGQv~k]c L 64n6F	qh/Bf Lj	B]KP!~+aH& %\vyoCd<x	  OC$f
.-l +~[ WJ	 .A+DN'+ V	

]_z}~k;YE  Ye{		!
+
#6 	MWWt\			)
u&/x RK^	
m[!;Dt\*%   @4f1EyOzow08@LQ_u % % #t+wL(QcDuW ) > 1}	$

'Vw
LU^bx0pz@#0A% G a a (
[
`

27A]%;(?-B    D^Y~Q &N( . 1  Qv		.

?<>{LIs)U:Z|p   ejJ_Mh

fxbs+dn3   r"/=74O	

tbvDy
  z	8hqd>}  T["1@
,3a w gFg'			!^T >Uqb3	u
l)F? Y' -N~#d	b			~
'ms5Thcf$    MG =yX
Z#E\aZ&-;=s  T	" `			vo#k{ ( 5  tHmYDu  Jq+t	r
rr * 7  )Rd%G
iJ]3N!  <-^Mw		i
O*01cQ F( W


&,Tnhr5TXi{>R/i+}^ef#   v
kBGO'pN`q   
qlvz v ${~	CKI[d|q& `m	P
TQ65 ^ z  3w.s+;bUo{6;$  h  h	

69\ 3$h   |)K
 ,5Pa	w	 2 '.nw1	


0J"El,)^*7[I  |)g? 
(^EHJL*4: Ulm	vYZ
F>SY' BRo;%	f<T99byBsw   =9		:gXJ2a C[	&QUc   %?-U^	
FP{  $Bi	p *e	A,k V 
y*jC'  "L*sJ[	%P0t :nv7Kt  #  P|} z&K@T]rTw%Y@  !gv ,E~LTF}	t
l4e,~r ' 4  s*s	j# ]VLv	N/SNIdMep u v-lr	~BT"  &f	

/!]+(6g  T<	P8XI	=X  >#~ITh		
i)J+ S\$iu e	O7MSLWo }g	(htJK  0L  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                      -                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
                                                                                                                              
                                                                                                                                      ,               	                                                                     !                                                                        
   	                      	                  	            '      
            
   
   	                                       	                                             	               '   )      (   $         $            z      !   ^   ^   *         3   e                                                                                                                                                                           -                                                                                                                                                                                                                                                                                                 
                                                  
                                                      ,     	                         !                          
 	        	      	    '  
    
 
 	             	               	     ' )  ( $   $    z  ! ^ ^ *   3 e   :  j         X  
u I	*r)*t"/1 #q
^OR4ccccXvS 'OR[dS<;&iB


 5I&jZc eDkDN1	> y	(y	( =r=v*	g
wQk{3^G<I  *ry	Cx|o
oCo
l o
Cb	Yr.	}$^ sTruCwx.ArW	= 6<d,}|S\Lz $]EOk<kU		o
oCof&		=J24 $QF
yKmqu2EOSDX\e
	,"xh6t	mNCD.K  *.  
I kOIdn
k(o

F g  f"\cK&			!eU Aj$EO .
<:Z|K  lZ| l6	$XZ[Te
o1Mdixb6 #zii hp YZ5		8	
XK 	m9i|&T]wI@M	#		$	d- 0  	9F&o{M_ <'R	?Zo"F >~T	
		:~p'- 0  	9{F&oM_ LF,~r]	| -} x )- 0  !p)r	T
vOX9F&ojm{M_bs  >~T|	
&T]wI@MxW
Y<&c2i

4j'E
x) 6 ($h } %

i/ M y-B<T9i
O1Q ' 		
+4.h*B~	i
<OT1 9P[Q	DQ ( 5 tD>PsX*
IJX0
:
0mD	 :PLeOY6 ;A	Z
:'>,Abj f]G6oB	T
:  k	V(i@W   74Z{>'G K)3	 	W:PLe!p)rT
vOXjmbs( 5 DtQ D Rf!b!C_fa$0UB	MWWt\			)
u&/x "L*sJ[	%P0t :nv7Kt  X  Xp,		
0
:
T
>v=0XmyI<Djbi~X[f
<p2N> l7		
dRt[j}1$D\qS 
s]		(

v'.DCx)y/P^  
qlvz #+y]zci7=As <JqTR	{x)@NZpR|"Gl  %?-U^	
FP{ gFg'			!^T2 s ~   '<AJRlRq+PT/7Rj			
E
I

{dR(x)M@>NZpt*NRV|"4%G[	3jl}1G$D\^qSQ gFj>aNU8gq=Qbn'	3				Q
u
lC!/)s 6F^?x7 )C68:TY6Udz	
-|"} Heo\	p
6X058xqbz   %?-P]VLU^|v		
} N/z&KS@T]rFPTwN%IYd@Mep{  * 7 >   %1?-)Rd%U^}		
$
G

'Vw
LU^FPbx0ipz@J#]30AN{ 6|4Y
_B?H=hJm~T<	P8XI	=XM 7]oP	94X}*9y	L


r
n@{-!OV\x9N   U28g ld,7u	z					

:Td-R|."}_t}y[!jq} 1$D\q S5[mnc\O-.G  K               .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NA^fթaNx01Si:YQ^{GdeyUVVWx9 ( ב!U^hQi՚vme^5V*Dؙ*ku[UՑFPd:bFfu^LFdcV&XG5efUa[wVyNUYeJגUhjJidWJjkZJYXԓ;eeץn:XU^EVdXxv˖CgXg`\%:VeU!t +GN^aZ]?RT%i`=M"9F_JV,aXd^fZSR4Fܕ
}TU}Y(m	;[b8!Z)ֽvq<R%O#億Z2Wial=s0C
;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%    i  s    !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  6<?xml version="1.0"?><Features recycledIDs="5,14,7,10,15,2" nextValidID="46"><Feature recentID="6" name="DFTL_0545_FR1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6762" color="#a6acb3" type="standard"/></Feature><Feature recentID="18" name="geranyltranstransferase" directionality="1" translationMW="22172.21" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="96" hitsStopCodon="1"><Segment range="6108-6706" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="20" name="FTL_0546" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6706" color="#00e600" type="standard"/></Feature><Feature recentID="13" name="phosphatidylcholine synthase" directionality="1" translationMW="376.49" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6780" maxFusedRunOn="6780"><Segment range="6754-6762" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="12" name="FTL_0545" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6754-6762" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6762" color="#000000" type="standard"/></Feature><Feature recentID="25" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6772-7396" color="#a6acb3" type="standard"/></Feature><Feature recentID="32" name="tRNA-dihydrouridine synthase A" directionality="1" translationMW="22327.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7500" maxFusedRunOn="7500"><Segment range="6814-7396" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="9" name="FTL_1549" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6814-7396" color="#00e600" type="standard"/></Feature><Feature recentID="8" name="hypothetical protein" directionality="1" translationMW="228.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="265" hitsStopCodon="1"><Segment range="6772-6780" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="4" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6772-7396" color="#000000" type="standard"/></Feature><Feature recentID="45" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7447-7463" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="44" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7423-7439" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="43" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="42" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7516-7537" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="7471-7477" color="#ffffff" type="standard"/><Segment range="7478-7495" color="#ffffff" type="standard"/><Segment name="-35" range="7496-7501" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="36" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7825-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="30" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="29" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="28" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="27" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7434-7459" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="26" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7456-7475" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="24" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="23" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="22" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="21" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="19" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="17" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="11" name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6763-6767" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q></Feature><Feature recentID="3" name="FTL_0404" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7397-7401" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="3952331" predef="GeneID"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8125" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL126 pEXDFTL_0402"/></Q></Feature></Features>
  
d<?xml version="1.0"?><Primers recycledIDs="2,3,4" nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="6" name="P797 DFTL_0545FR1R" sequence="tacttgcattttaatattaacttttaaataaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6730-6761" boundStrand="1" annealedBases="tacttgcattttaatattaacttttaaataaa" meltingTemperature="50"><Component hybridizedRange="6730-6761" bases="tacttgcattttaatattaacttttaaataaa"/></BindingSite><BindingSite simplified="1" location="6730-6761" boundStrand="1" annealedBases="tacttgcattttaatattaacttttaaataaa" meltingTemperature="50"><Component hybridizedRange="6730-6761" bases="tacttgcattttaatattaacttttaaataaa"/></BindingSite></Primer><Primer recentID="5" name="P796 DFTL_0545FR1F" sequence="caacttgtcatatcaaatttaatgaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6107-6134" boundStrand="0" annealedBases="caacttgtcatatcaaatttaatgaagc" meltingTemperature="54"><Component hybridizedRange="6107-6134" bases="caacttgtcatatcaaatttaatgaagc"/></BindingSite><BindingSite simplified="1" location="6107-6134" boundStrand="0" annealedBases="caacttgtcatatcaaatttaatgaagc" meltingTemperature="54"><Component hybridizedRange="6107-6134" bases="caacttgtcatatcaaatttaatgaagc"/></BindingSite></Primer><Primer recentID="1" name="P791 DFTL_1548FR2F" sequence="GCGGCCGCTcctttataattttataacaacttaaatctc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6762-6800" boundStrand="0" annealedBases="GCGGCCGCTcctttataattttataacaacttaaatctc" meltingTemperature="62"><Component hybridizedRange="6762-6800" bases="GCGGCCGCTcctttataattttataacaacttaaatctc"/></BindingSite><BindingSite simplified="1" location="6762-6800" boundStrand="0" annealedBases="GCGGCCGCTcctttataattttataacaacttaaatctc" meltingTemperature="62"><Component hybridizedRange="6762-6800" bases="GCGGCCGCTcctttataattttataacaacttaaatctc"/></BindingSite></Primer><Primer recentID="0" name="P792 DFTL_1548FR2R" sequence="GGTACCtgatactgtagacaacatcatatttaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7368-7401" boundStrand="1" annealedBases="GGTACCtgatactgtagacaacatcatatttaag" meltingTemperature="58"><Component hybridizedRange="7368-7401" bases="GGTACCtgatactgtagacaacatcatatttaag"/></BindingSite><BindingSite simplified="1" location="7368-7401" boundStrand="1" annealedBases="GGTACCtgatactgtagacaacatcatatttaag" meltingTemperature="58"><Component hybridizedRange="7368-7401" bases="GGTACCtgatactgtagacaacatcatatttaag"/></BindingSite></Primer></Primers>
  A<Notes>
<UUID>20cc3d38-202f-4210-9cdc-449084c0e81a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:54:14">2018.6.6</Created>
<LastModified UTC="13:24:45">2018.6.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  O     )            .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAmRVrT+鸡}zx{]d|Z)zS6^]%|gJmz}xY~?YYigFtOoYzmY}9CiYaO9	gkкM_IYa-kvWZ)ZRiuenXX*oUci{JFBVUC;eeץn:XU^EVdXxv˖CgXg`\%:VeU!t +GN^aZ]?RT%i`=M"9F_JV,aXd^fZSR4Fܕ
}TU}Y(m	;[b8!Z)ֽvq<R%O#億Z2Wial=s0C
;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%        ?  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  5<?xml version="1.0"?><Features recycledIDs="6,5,14,7,10,15,2" nextValidID="46"><Feature recentID="18" name="DFTL_1548_FR1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6854" color="#a6acb3" type="standard"/></Feature><Feature recentID="20" name="FTL_1545 hypothetical protein" directionality="1" translationMW="387.52" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6872" maxFusedRunOn="6872"><Segment range="6846-6854" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="13" name="DNA gyrase subunit B" directionality="2" translationMW="20114.97" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="5858" maxFusedRunOn="5858"><Segment range="6108-6664" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="12" name="FTL_1547" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6664" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6854" color="#000000" type="standard"/></Feature><Feature recentID="25" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6864-7488" color="#a6acb3" type="standard"/></Feature><Feature recentID="32" name="tRNA-dihydrouridine synthase A" directionality="1" translationMW="22327.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7592" maxFusedRunOn="7592"><Segment range="6906-7488" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="9" name="FTL_1549" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6906-7488" color="#00e600" type="standard"/></Feature><Feature recentID="8" name="hypothetical protein" directionality="1" translationMW="228.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="265" hitsStopCodon="1"><Segment range="6864-6872" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="4" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6864-7488" color="#000000" type="standard"/></Feature><Feature recentID="45" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7539-7555" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="44" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7515-7531" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="43" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="42" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7608-7629" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="7563-7569" color="#ffffff" type="standard"/><Segment range="7570-7587" color="#ffffff" type="standard"/><Segment name="-35" range="7588-7593" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="36" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7917-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="30" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="29" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="28" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="27" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7526-7551" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="26" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7548-7567" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="24" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="23" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="22" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="21" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="19" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="17" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="11" name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6855-6859" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q></Feature><Feature recentID="3" name="FTL_0404" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7489-7493" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="3952331" predef="GeneID"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8217" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL126 pEXDFTL_0402"/></Q></Feature></Features>
  .<?xml version="1.0"?><Primers nextValidID="5"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="4" name="P793 DFTL_1548_int1" sequence="atagcattatcaacaacctcaaatacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6515-6541" boundStrand="0" annealedBases="atagcattatcaacaacctcaaatacc" meltingTemperature="56"><Component hybridizedRange="6515-6541" bases="atagcattatcaacaacctcaaatacc"/></BindingSite><BindingSite simplified="1" location="6515-6541" boundStrand="0" annealedBases="atagcattatcaacaacctcaaatacc" meltingTemperature="56"><Component hybridizedRange="6515-6541" bases="atagcattatcaacaacctcaaatacc"/></BindingSite></Primer><Primer recentID="3" name="P790 DFTL_1548FR1R" sequence="GCGGCCGCctttttcaaaatttcaaaatgaacctatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6825-6861" boundStrand="1" annealedBases="GCGGCCGCctttttcaaaatttcaaaatgaacctatc" meltingTemperature="65"><Component hybridizedRange="6825-6861" bases="GCGGCCGCctttttcaaaatttcaaaatgaacctatc"/></BindingSite><BindingSite simplified="1" location="6825-6861" boundStrand="1" annealedBases="GCGGCCGCctttttcaaaatttcaaaatgaacctatc" meltingTemperature="65"><Component hybridizedRange="6825-6861" bases="GCGGCCGCctttttcaaaatttcaaaatgaacctatc"/></BindingSite></Primer><Primer recentID="2" name="P789 DFTL_1548FR1F" sequence="GGATCCtcataatcaaagtcaacaaatgaaaacg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6101-6134" boundStrand="0" annealedBases="GGATCCtcataatcaaagtcaacaaatgaaaacg" meltingTemperature="60"><Component hybridizedRange="6101-6134" bases="GGATCCtcataatcaaagtcaacaaatgaaaacg"/></BindingSite><BindingSite location="2698-2711" boundStrand="1" annealedBases="aacaaataaaacg" meltingTemperature="30"><Component bases="GGATCCtcataatcaaagtc"/><Component hybridizedRange="2705-2711" bases="aacaaat"/><Component bases="g"/><Component hybridizedRange="2698-2703" bases="aaaacg"/></BindingSite><BindingSite simplified="1" location="6101-6134" boundStrand="0" annealedBases="GGATCCtcataatcaaagtcaacaaatgaaaacg" meltingTemperature="60"><Component hybridizedRange="6101-6134" bases="GGATCCtcataatcaaagtcaacaaatgaaaacg"/></BindingSite><BindingSite simplified="1" location="2698-2711" boundStrand="1" annealedBases="aacaaataaaacg" meltingTemperature="30"><Component bases="GGATCCtcataatcaaagtc"/><Component hybridizedRange="2705-2711" bases="aacaaat"/><Component bases="g"/><Component hybridizedRange="2698-2703" bases="aaaacg"/></BindingSite></Primer><Primer recentID="1" name="P791 DFTL_1548FR2F" sequence="GCGGCCGCTcctttataattttataacaacttaaatctc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6854-6892" boundStrand="0" annealedBases="GCGGCCGCTcctttataattttataacaacttaaatctc" meltingTemperature="62"><Component hybridizedRange="6854-6892" bases="GCGGCCGCTcctttataattttataacaacttaaatctc"/></BindingSite><BindingSite simplified="1" location="6854-6892" boundStrand="0" annealedBases="GCGGCCGCTcctttataattttataacaacttaaatctc" meltingTemperature="62"><Component hybridizedRange="6854-6892" bases="GCGGCCGCTcctttataattttataacaacttaaatctc"/></BindingSite></Primer><Primer recentID="0" name="P792 DFTL_1548FR2R" sequence="GGTACCtgatactgtagacaacatcatatttaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7460-7493" boundStrand="1" annealedBases="GGTACCtgatactgtagacaacatcatatttaag" meltingTemperature="58"><Component hybridizedRange="7460-7493" bases="GGTACCtgatactgtagacaacatcatatttaag"/></BindingSite><BindingSite simplified="1" location="7460-7493" boundStrand="1" annealedBases="GGTACCtgatactgtagacaacatcatatttaag" meltingTemperature="58"><Component hybridizedRange="7460-7493" bases="GGTACCtgatactgtagacaacatcatatttaag"/></BindingSite></Primer></Primers>
  @<Notes>
<UUID>20cc3d38-202f-4210-9cdc-449084c0e81a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:54:14">2018.6.6</Created>
<LastModified UTC="13:24:9">2018.6.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  M               .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAͩVV&`=eiꍚ՛en]R%J!Ueng1Mm(&NemY+EF}{ugUzIXYS;eeץn:XU^EVdXxv˖CgXg`\%:VeU!t +GN^aZ]?RT%i`=M"9F_JV,aXd^fZSR4Fܕ
}TU}Y(m	;[b8!Z)ֽvq<R%O#億Z2Wial=s0C
;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%    Y  c    !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  >&<?xml version="1.0"?><Features recycledIDs="2,20,18" nextValidID="46"><Feature recentID="25" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6500-7124" color="#a6acb3" type="standard"/></Feature><Feature recentID="32" name="tRNA-dihydrouridine synthase A" directionality="1" translationMW="22327.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7228" maxFusedRunOn="7228"><Segment range="6542-7124" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="9" name="FTL_1549" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6542-7124" color="#00e600" type="standard"/></Feature><Feature recentID="8" name="hypothetical protein" directionality="1" translationMW="228.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="265" hitsStopCodon="1"><Segment range="6500-6508" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="FTL_1548" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6500-6508" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6500-7124" color="#000000" type="standard"/></Feature><Feature recentID="45" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7175-7191" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="44" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7151-7167" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="43" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="42" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7244-7265" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="7199-7205" color="#ffffff" type="standard"/><Segment range="7206-7223" color="#ffffff" type="standard"/><Segment name="-35" range="7224-7229" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="36" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7553-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="30" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="29" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="28" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="27" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7162-7187" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="26" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7184-7203" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="24" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="23" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="22" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="21" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="19" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="17" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="15" name="P774 DFTL_0402FR1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6107-6141" color="#a020f0" type="standard"/><Q name="note"><V text="P774 DFTL_0402FR1F"/></Q></Feature><Feature recentID="14" name="P775 DFTL_0402FR1R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6465-6499" color="#a020f0" type="standard"/><Q name="note"><V text="P775 DFTL_0402FR1R"/></Q></Feature><Feature recentID="13" name="FTL_0401" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6426" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0401"/></Q><Q name="db_xref"><V text="3952328" predef="GeneID"/></Q></Feature><Feature recentID="12" name="FTL_0401" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6113-6426" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="product"><V text="Sua5_yciO_yrdC family protein"/></Q><Q name="note"><V text="/dnas_title=Sua5_yciO_yrdC family protein"/></Q><Q name="locus_tag"><V text="FTL_0401"/></Q><Q name="db_xref"><V text="89255812" predef="GI"/><V text="3952328" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513174.1"/></Q></Feature><Feature recentID="11" name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6487-6495" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q></Feature><Feature recentID="10" name="FTL_0402" directionality="1" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6516" maxFusedRunOn="6516"><Segment range="6487-6495" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="product"><V text="intracellular septation protein A family&#10;protein"/></Q><Q name="note"><V text="/dnas_title=intracellular septation protein A family&#10;protein"/></Q><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="89255813" predef="GI"/><V text="3952329" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513175.1"/></Q></Feature><Feature recentID="7" name="P776 DFTL_0402FR2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6496-6499" color="#a020f0" type="standard"/><Q name="note"><V text="P776 DFTL_0402FR2F"/></Q></Feature><Feature recentID="6" name="P777 DFTL_0402FR2R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7125-7135" color="#a020f0" type="standard"/><Q name="note"><V text="P777 DFTL_0402FR2R"/></Q></Feature><Feature recentID="3" name="FTL_0404" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7125-7129" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="3952331" predef="GeneID"/></Q></Feature><Feature recentID="1" name="P778 CkDFTL_0402F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6425-6452" color="#a020f0" type="standard"/><Q name="note"><V text="P778 CkDFTL_0402F"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7853" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL126 pEXDFTL_0402"/></Q></Feature></Features>
  I<?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="P791 DFTL_1548FR2F" sequence="cctttataattttataacaacttaaatctc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6499-6528" boundStrand="0" annealedBases="cctttataattttataacaacttaaatctc" meltingTemperature="51"><Component hybridizedRange="6499-6528" bases="cctttataattttataacaacttaaatctc"/></BindingSite><BindingSite simplified="1" location="6499-6528" boundStrand="0" annealedBases="cctttataattttataacaacttaaatctc" meltingTemperature="51"><Component hybridizedRange="6499-6528" bases="cctttataattttataacaacttaaatctc"/></BindingSite></Primer><Primer recentID="0" name="P792 DFTL_1548FR2R" sequence="tgatactgtagacaacatcatatttaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7096-7123" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="52"><Component hybridizedRange="7096-7123" bases="tgatactgtagacaacatcatatttaag"/></BindingSite><BindingSite simplified="1" location="7096-7123" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="52"><Component hybridizedRange="7096-7123" bases="tgatactgtagacaacatcatatttaag"/></BindingSite></Primer></Primers>
  A<Notes>
<UUID>20cc3d38-202f-4210-9cdc-449084c0e81a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:54:14">2018.6.6</Created>
<LastModified UTC="12:59:54">2018.6.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  E     )  <        <.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAͩVV&`=eiꍚ՛en]R%J!Ueng1Mm(&NemY+EF}{ugUzIXYS8'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ    Y  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ;G<?xml version="1.0"?><Features recycledIDs="4,5,8,9,32,25,20,18" nextValidID="46"><Feature recentID="45" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6550-6566" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="44" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6526-6542" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="43" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="42" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6619-6640" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="6574-6580" color="#ffffff" type="standard"/><Segment range="6581-6598" color="#ffffff" type="standard"/><Segment name="-35" range="6599-6604" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="36" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6928-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="30" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="29" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="28" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="27" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6537-6562" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="26" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6559-6578" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="24" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="23" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="22" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="21" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="19" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="17" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="15" name="P774 DFTL_0402FR1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6107-6141" color="#a020f0" type="standard"/><Q name="note"><V text="P774 DFTL_0402FR1F"/></Q></Feature><Feature recentID="14" name="P775 DFTL_0402FR1R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6465-6499" color="#a020f0" type="standard"/><Q name="note"><V text="P775 DFTL_0402FR1R"/></Q></Feature><Feature recentID="13" name="FTL_0401" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6426" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0401"/></Q><Q name="db_xref"><V text="3952328" predef="GeneID"/></Q></Feature><Feature recentID="12" name="FTL_0401" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6113-6426" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="product"><V text="Sua5_yciO_yrdC family protein"/></Q><Q name="note"><V text="/dnas_title=Sua5_yciO_yrdC family protein"/></Q><Q name="locus_tag"><V text="FTL_0401"/></Q><Q name="db_xref"><V text="89255812" predef="GI"/><V text="3952328" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513174.1"/></Q></Feature><Feature recentID="11" name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6487-6495" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q></Feature><Feature recentID="10" name="FTL_0402" directionality="1" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6603" maxFusedRunOn="6603"><Segment range="6487-6495" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="product"><V text="intracellular septation protein A family&#10;protein"/></Q><Q name="note"><V text="/dnas_title=intracellular septation protein A family&#10;protein"/></Q><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="89255813" predef="GI"/><V text="3952329" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513175.1"/></Q></Feature><Feature recentID="7" name="P776 DFTL_0402FR2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6496-6499" color="#a020f0" type="standard"/><Q name="note"><V text="P776 DFTL_0402FR2F"/></Q></Feature><Feature recentID="6" name="P777 DFTL_0402FR2R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6500-6510" color="#a020f0" type="standard"/><Q name="note"><V text="P777 DFTL_0402FR2R"/></Q></Feature><Feature recentID="3" name="FTL_0404" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6500-6504" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="3952331" predef="GeneID"/></Q></Feature><Feature recentID="2" name="FTL_0404" directionality="2" translateFirstCodonAsMet="1" translationMW="117.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6439" maxFusedRunOn="6439"><Segment range="6500-6504" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="product"><V text="glucose kinase"/></Q><Q name="note"><V text="/dnas_title=glucose kinase"/></Q><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="89255815" predef="GI"/><V text="3952331" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513177.1"/></Q><Q name="EC_number"><V text="2.7.1.2"/></Q></Feature><Feature recentID="1" name="P778 CkDFTL_0402F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6425-6452" color="#a020f0" type="standard"/><Q name="note"><V text="P778 CkDFTL_0402F"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7228" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL126 pEXDFTL_0402"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  A<Notes>
<UUID>20cc3d38-202f-4210-9cdc-449084c0e81a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:54:14">2018.6.6</Created>
<LastModified UTC="12:59:41">2018.6.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  N      /   2         2.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAͩVV&`=eiꍚ՛en]R%J!Ueng1Mm(&NemY+EF}{ugUzIXYS:Zڤ5f&ZMuieUVhu_UYSeWfn*IifHNgBj9櫙t&9ڧBiUYZVg'3>i_i[mʇkE#YYT%z%#^ٙEXႻdu>DWyYs{СeA7ݞYV<t[A`TsezV^ע]Eove{mݯMMJm!nnDk_Z|NŬضN*Vo;uw]O6TSa!9{v;x=8X[\£&ξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	    Y  c  X  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  C<?xml version="1.0"?><Features recycledIDs="32,25,20,18" nextValidID="46"><Feature recentID="45" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7564-7580" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="44" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7540-7556" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="43" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="42" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7633-7654" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="7588-7594" color="#ffffff" type="standard"/><Segment range="7595-7612" color="#ffffff" type="standard"/><Segment name="-35" range="7613-7618" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="36" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7942-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="30" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="29" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="28" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="27" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7551-7576" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="26" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7573-7592" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="24" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="23" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="22" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="21" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="19" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="17" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="15" name="P774 DFTL_0402FR1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6107-6141" color="#a020f0" type="standard"/><Q name="note"><V text="P774 DFTL_0402FR1F"/></Q></Feature><Feature recentID="14" name="P775 DFTL_0402FR1R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6465-6503" color="#a020f0" type="standard"/><Q name="note"><V text="P775 DFTL_0402FR1R"/></Q></Feature><Feature recentID="13" name="FTL_0401" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6426" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0401"/></Q><Q name="db_xref"><V text="3952328" predef="GeneID"/></Q></Feature><Feature recentID="12" name="FTL_0401" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6113-6426" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="product"><V text="Sua5_yciO_yrdC family protein"/></Q><Q name="note"><V text="/dnas_title=Sua5_yciO_yrdC family protein"/></Q><Q name="locus_tag"><V text="FTL_0401"/></Q><Q name="db_xref"><V text="89255812" predef="GI"/><V text="3952328" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513174.1"/></Q></Feature><Feature recentID="11" name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6487-6495" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q></Feature><Feature recentID="10" name="FTL_0402" directionality="1" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6516" maxFusedRunOn="6516"><Segment range="6487-6495" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="product"><V text="intracellular septation protein A family&#10;protein"/></Q><Q name="note"><V text="/dnas_title=intracellular septation protein A family&#10;protein"/></Q><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="89255813" predef="GI"/><V text="3952329" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513175.1"/></Q></Feature><Feature recentID="9" name="FTL_0403" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6581-6832" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0403"/></Q><Q name="db_xref"><V text="3952330" predef="GeneID"/></Q></Feature><Feature recentID="8" name="FTL_0403" directionality="1" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6877" maxFusedRunOn="6877"><Segment range="6581-6832" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V predef="ab initio prediction"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="locus_tag"><V text="FTL_0403"/></Q><Q name="db_xref"><V text="89255814" predef="GI"/><V text="3952330" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513176.1"/></Q></Feature><Feature recentID="7" name="P776 DFTL_0402FR2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6496-6534" color="#a020f0" type="standard"/><Q name="note"><V text="P776 DFTL_0402FR2F"/></Q></Feature><Feature recentID="6" name="P777 DFTL_0402FR2R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7492-7524" color="#a020f0" type="standard"/><Q name="note"><V text="P777 DFTL_0402FR2R"/></Q></Feature><Feature recentID="5" name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6505-6507" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q></Feature><Feature recentID="4" name="FTL_0402" directionality="1" translateFirstCodonAsMet="1" translationMW="131.18" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6516" maxFusedRunOn="6516"><Segment range="6505-6507" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="product"><V text="intracellular septation protein A family&#10;protein"/></Q><Q name="note"><V text="/dnas_title=intracellular septation protein A family&#10;protein"/></Q><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="89255813" predef="GI"/><V text="3952329" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513175.1"/></Q></Feature><Feature recentID="3" name="FTL_0404" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6821-7518" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="3952331" predef="GeneID"/></Q></Feature><Feature recentID="2" name="FTL_0404" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6811" maxFusedRunOn="6811"><Segment range="6821-7518" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="product"><V text="glucose kinase"/></Q><Q name="note"><V text="/dnas_title=glucose kinase"/></Q><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="89255815" predef="GI"/><V text="3952331" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513177.1"/></Q><Q name="EC_number"><V text="2.7.1.2"/></Q></Feature><Feature recentID="1" name="P778 CkDFTL_0402F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6425-6452" color="#a020f0" type="standard"/><Q name="note"><V text="P778 CkDFTL_0402F"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8242" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL126 pEXDFTL_0402"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  A<Notes>
<UUID>20cc3d38-202f-4210-9cdc-449084c0e81a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:54:14">2018.6.6</Created>
<LastModified UTC="12:56:38">2018.6.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  K               .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NA^fթaNx01Si:YQ^{GdeyUVVWx9 ( ב!U^hQi՚vme^5V*Dؙ*ku[UՑFPd:bFfu^LFdcV&XG5efUa[wVyNUYeJגUhjJidWJjkZJYXԓ;eeץn:XU^EVdXxv˖CgXg`\%:VeU!t +GN^aZ]?RT%i`=M"9F_JV,aXd^fZSR4Fܕ
}TU}Y(m	;[b8!Z)ֽvq<R%O#億Z2Wial=s0C
;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%    i  s    !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  6<?xml version="1.0"?><Features recycledIDs="5,14,7,10,15,2" nextValidID="46"><Feature recentID="6" name="DFTL_0545_FR1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6762" color="#a6acb3" type="standard"/></Feature><Feature recentID="18" name="geranyltranstransferase" directionality="1" translationMW="22172.21" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="96" hitsStopCodon="1"><Segment range="6108-6706" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="20" name="FTL_0546" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6706" color="#00e600" type="standard"/></Feature><Feature recentID="13" name="phosphatidylcholine synthase" directionality="1" translationMW="376.49" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6780" maxFusedRunOn="6780"><Segment range="6754-6762" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="12" name="FTL_0545" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6754-6762" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6762" color="#000000" type="standard"/></Feature><Feature recentID="25" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6772-7396" color="#a6acb3" type="standard"/></Feature><Feature recentID="32" name="tRNA-dihydrouridine synthase A" directionality="1" translationMW="22327.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7500" maxFusedRunOn="7500"><Segment range="6814-7396" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="9" name="FTL_1549" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6814-7396" color="#00e600" type="standard"/></Feature><Feature recentID="8" name="hypothetical protein" directionality="1" translationMW="228.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="265" hitsStopCodon="1"><Segment range="6772-6780" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="4" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6772-7396" color="#000000" type="standard"/></Feature><Feature recentID="45" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7447-7463" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="44" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7423-7439" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="43" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="42" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7516-7537" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="7471-7477" color="#ffffff" type="standard"/><Segment range="7478-7495" color="#ffffff" type="standard"/><Segment name="-35" range="7496-7501" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="36" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7825-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="30" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="29" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="28" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="27" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7434-7459" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="26" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7456-7475" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="24" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="23" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="22" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="21" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="19" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="17" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="11" name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6763-6767" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q></Feature><Feature recentID="3" name="FTL_0404" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7397-7401" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="3952331" predef="GeneID"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8125" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL126 pEXDFTL_0402"/></Q></Feature></Features>
  
d<?xml version="1.0"?><Primers recycledIDs="2,3,4" nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="6" name="P797 DFTL_0545FR1R" sequence="tacttgcattttaatattaacttttaaataaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6730-6761" boundStrand="1" annealedBases="tacttgcattttaatattaacttttaaataaa" meltingTemperature="50"><Component hybridizedRange="6730-6761" bases="tacttgcattttaatattaacttttaaataaa"/></BindingSite><BindingSite simplified="1" location="6730-6761" boundStrand="1" annealedBases="tacttgcattttaatattaacttttaaataaa" meltingTemperature="50"><Component hybridizedRange="6730-6761" bases="tacttgcattttaatattaacttttaaataaa"/></BindingSite></Primer><Primer recentID="5" name="P796 DFTL_0545FR1F" sequence="caacttgtcatatcaaatttaatgaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6107-6134" boundStrand="0" annealedBases="caacttgtcatatcaaatttaatgaagc" meltingTemperature="54"><Component hybridizedRange="6107-6134" bases="caacttgtcatatcaaatttaatgaagc"/></BindingSite><BindingSite simplified="1" location="6107-6134" boundStrand="0" annealedBases="caacttgtcatatcaaatttaatgaagc" meltingTemperature="54"><Component hybridizedRange="6107-6134" bases="caacttgtcatatcaaatttaatgaagc"/></BindingSite></Primer><Primer recentID="1" name="P791 DFTL_1548FR2F" sequence="GCGGCCGCTcctttataattttataacaacttaaatctc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6762-6800" boundStrand="0" annealedBases="GCGGCCGCTcctttataattttataacaacttaaatctc" meltingTemperature="62"><Component hybridizedRange="6762-6800" bases="GCGGCCGCTcctttataattttataacaacttaaatctc"/></BindingSite><BindingSite simplified="1" location="6762-6800" boundStrand="0" annealedBases="GCGGCCGCTcctttataattttataacaacttaaatctc" meltingTemperature="62"><Component hybridizedRange="6762-6800" bases="GCGGCCGCTcctttataattttataacaacttaaatctc"/></BindingSite></Primer><Primer recentID="0" name="P792 DFTL_1548FR2R" sequence="GGTACCtgatactgtagacaacatcatatttaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7368-7401" boundStrand="1" annealedBases="GGTACCtgatactgtagacaacatcatatttaag" meltingTemperature="58"><Component hybridizedRange="7368-7401" bases="GGTACCtgatactgtagacaacatcatatttaag"/></BindingSite><BindingSite simplified="1" location="7368-7401" boundStrand="1" annealedBases="GGTACCtgatactgtagacaacatcatatttaag" meltingTemperature="58"><Component hybridizedRange="7368-7401" bases="GGTACCtgatactgtagacaacatcatatttaag"/></BindingSite></Primer></Primers>
  A<Notes>
<UUID>20cc3d38-202f-4210-9cdc-449084c0e81a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:54:14">2018.6.6</Created>
<LastModified UTC="13:24:45">2018.6.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  O     )            .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAmRVrT+鸡}zx{]d|Z)zS6^]%|gJmz}xY~?YYigFtOoYzmY}9CiYaO9	gkкM_IYa-kvWZ)ZRiuenXX*oUci{JFBVUC;eeץn:XU^EVdXxv˖CgXg`\%:VeU!t +GN^aZ]?RT%i`=M"9F_JV,aXd^fZSR4Fܕ
}TU}Y(m	;[b8!Z)ֽvq<R%O#億Z2Wial=s0C
;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%        ?  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  5<?xml version="1.0"?><Features recycledIDs="6,5,14,7,10,15,2" nextValidID="46"><Feature recentID="18" name="DFTL_1548_FR1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6854" color="#a6acb3" type="standard"/></Feature><Feature recentID="20" name="FTL_1545 hypothetical protein" directionality="1" translationMW="387.52" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6872" maxFusedRunOn="6872"><Segment range="6846-6854" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="13" name="DNA gyrase subunit B" directionality="2" translationMW="20114.97" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="5858" maxFusedRunOn="5858"><Segment range="6108-6664" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="12" name="FTL_1547" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6664" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6854" color="#000000" type="standard"/></Feature><Feature recentID="25" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6864-7488" color="#a6acb3" type="standard"/></Feature><Feature recentID="32" name="tRNA-dihydrouridine synthase A" directionality="1" translationMW="22327.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7592" maxFusedRunOn="7592"><Segment range="6906-7488" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="9" name="FTL_1549" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6906-7488" color="#00e600" type="standard"/></Feature><Feature recentID="8" name="hypothetical protein" directionality="1" translationMW="228.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="265" hitsStopCodon="1"><Segment range="6864-6872" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="4" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6864-7488" color="#000000" type="standard"/></Feature><Feature recentID="45" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7539-7555" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="44" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7515-7531" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="43" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="42" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7608-7629" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="7563-7569" color="#ffffff" type="standard"/><Segment range="7570-7587" color="#ffffff" type="standard"/><Segment name="-35" range="7588-7593" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="36" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7917-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="30" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="29" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="28" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="27" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7526-7551" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="26" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7548-7567" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="24" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="23" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="22" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="21" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="19" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="17" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="11" name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6855-6859" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q></Feature><Feature recentID="3" name="FTL_0404" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7489-7493" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="3952331" predef="GeneID"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8217" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL126 pEXDFTL_0402"/></Q></Feature></Features>
  .<?xml version="1.0"?><Primers nextValidID="5"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="4" name="P793 DFTL_1548_int1" sequence="atagcattatcaacaacctcaaatacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6515-6541" boundStrand="0" annealedBases="atagcattatcaacaacctcaaatacc" meltingTemperature="56"><Component hybridizedRange="6515-6541" bases="atagcattatcaacaacctcaaatacc"/></BindingSite><BindingSite simplified="1" location="6515-6541" boundStrand="0" annealedBases="atagcattatcaacaacctcaaatacc" meltingTemperature="56"><Component hybridizedRange="6515-6541" bases="atagcattatcaacaacctcaaatacc"/></BindingSite></Primer><Primer recentID="3" name="P790 DFTL_1548FR1R" sequence="GCGGCCGCctttttcaaaatttcaaaatgaacctatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6825-6861" boundStrand="1" annealedBases="GCGGCCGCctttttcaaaatttcaaaatgaacctatc" meltingTemperature="65"><Component hybridizedRange="6825-6861" bases="GCGGCCGCctttttcaaaatttcaaaatgaacctatc"/></BindingSite><BindingSite simplified="1" location="6825-6861" boundStrand="1" annealedBases="GCGGCCGCctttttcaaaatttcaaaatgaacctatc" meltingTemperature="65"><Component hybridizedRange="6825-6861" bases="GCGGCCGCctttttcaaaatttcaaaatgaacctatc"/></BindingSite></Primer><Primer recentID="2" name="P789 DFTL_1548FR1F" sequence="GGATCCtcataatcaaagtcaacaaatgaaaacg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6101-6134" boundStrand="0" annealedBases="GGATCCtcataatcaaagtcaacaaatgaaaacg" meltingTemperature="60"><Component hybridizedRange="6101-6134" bases="GGATCCtcataatcaaagtcaacaaatgaaaacg"/></BindingSite><BindingSite location="2698-2711" boundStrand="1" annealedBases="aacaaataaaacg" meltingTemperature="30"><Component bases="GGATCCtcataatcaaagtc"/><Component hybridizedRange="2705-2711" bases="aacaaat"/><Component bases="g"/><Component hybridizedRange="2698-2703" bases="aaaacg"/></BindingSite><BindingSite simplified="1" location="6101-6134" boundStrand="0" annealedBases="GGATCCtcataatcaaagtcaacaaatgaaaacg" meltingTemperature="60"><Component hybridizedRange="6101-6134" bases="GGATCCtcataatcaaagtcaacaaatgaaaacg"/></BindingSite><BindingSite simplified="1" location="2698-2711" boundStrand="1" annealedBases="aacaaataaaacg" meltingTemperature="30"><Component bases="GGATCCtcataatcaaagtc"/><Component hybridizedRange="2705-2711" bases="aacaaat"/><Component bases="g"/><Component hybridizedRange="2698-2703" bases="aaaacg"/></BindingSite></Primer><Primer recentID="1" name="P791 DFTL_1548FR2F" sequence="GCGGCCGCTcctttataattttataacaacttaaatctc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6854-6892" boundStrand="0" annealedBases="GCGGCCGCTcctttataattttataacaacttaaatctc" meltingTemperature="62"><Component hybridizedRange="6854-6892" bases="GCGGCCGCTcctttataattttataacaacttaaatctc"/></BindingSite><BindingSite simplified="1" location="6854-6892" boundStrand="0" annealedBases="GCGGCCGCTcctttataattttataacaacttaaatctc" meltingTemperature="62"><Component hybridizedRange="6854-6892" bases="GCGGCCGCTcctttataattttataacaacttaaatctc"/></BindingSite></Primer><Primer recentID="0" name="P792 DFTL_1548FR2R" sequence="GGTACCtgatactgtagacaacatcatatttaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7460-7493" boundStrand="1" annealedBases="GGTACCtgatactgtagacaacatcatatttaag" meltingTemperature="58"><Component hybridizedRange="7460-7493" bases="GGTACCtgatactgtagacaacatcatatttaag"/></BindingSite><BindingSite simplified="1" location="7460-7493" boundStrand="1" annealedBases="GGTACCtgatactgtagacaacatcatatttaag" meltingTemperature="58"><Component hybridizedRange="7460-7493" bases="GGTACCtgatactgtagacaacatcatatttaag"/></BindingSite></Primer></Primers>
  @<Notes>
<UUID>20cc3d38-202f-4210-9cdc-449084c0e81a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:54:14">2018.6.6</Created>
<LastModified UTC="13:24:9">2018.6.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  M               .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAͩVV&`=eiꍚ՛en]R%J!Ueng1Mm(&NemY+EF}{ugUzIXYS;eeץn:XU^EVdXxv˖CgXg`\%:VeU!t +GN^aZ]?RT%i`=M"9F_JV,aXd^fZSR4Fܕ
}TU}Y(m	;[b8!Z)ֽvq<R%O#億Z2Wial=s0C
;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%    Y  c    !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  >&<?xml version="1.0"?><Features recycledIDs="2,20,18" nextValidID="46"><Feature recentID="25" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6500-7124" color="#a6acb3" type="standard"/></Feature><Feature recentID="32" name="tRNA-dihydrouridine synthase A" directionality="1" translationMW="22327.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7228" maxFusedRunOn="7228"><Segment range="6542-7124" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="9" name="FTL_1549" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6542-7124" color="#00e600" type="standard"/></Feature><Feature recentID="8" name="hypothetical protein" directionality="1" translationMW="228.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="265" hitsStopCodon="1"><Segment range="6500-6508" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="FTL_1548" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6500-6508" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6500-7124" color="#000000" type="standard"/></Feature><Feature recentID="45" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7175-7191" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="44" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7151-7167" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="43" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="42" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7244-7265" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="7199-7205" color="#ffffff" type="standard"/><Segment range="7206-7223" color="#ffffff" type="standard"/><Segment name="-35" range="7224-7229" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="36" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7553-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="30" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="29" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="28" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="27" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7162-7187" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="26" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7184-7203" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="24" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="23" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="22" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="21" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="19" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="17" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="15" name="P774 DFTL_0402FR1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6107-6141" color="#a020f0" type="standard"/><Q name="note"><V text="P774 DFTL_0402FR1F"/></Q></Feature><Feature recentID="14" name="P775 DFTL_0402FR1R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6465-6499" color="#a020f0" type="standard"/><Q name="note"><V text="P775 DFTL_0402FR1R"/></Q></Feature><Feature recentID="13" name="FTL_0401" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6426" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0401"/></Q><Q name="db_xref"><V text="3952328" predef="GeneID"/></Q></Feature><Feature recentID="12" name="FTL_0401" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6113-6426" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="product"><V text="Sua5_yciO_yrdC family protein"/></Q><Q name="note"><V text="/dnas_title=Sua5_yciO_yrdC family protein"/></Q><Q name="locus_tag"><V text="FTL_0401"/></Q><Q name="db_xref"><V text="89255812" predef="GI"/><V text="3952328" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513174.1"/></Q></Feature><Feature recentID="11" name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6487-6495" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q></Feature><Feature recentID="10" name="FTL_0402" directionality="1" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6516" maxFusedRunOn="6516"><Segment range="6487-6495" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="product"><V text="intracellular septation protein A family&#10;protein"/></Q><Q name="note"><V text="/dnas_title=intracellular septation protein A family&#10;protein"/></Q><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="89255813" predef="GI"/><V text="3952329" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513175.1"/></Q></Feature><Feature recentID="7" name="P776 DFTL_0402FR2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6496-6499" color="#a020f0" type="standard"/><Q name="note"><V text="P776 DFTL_0402FR2F"/></Q></Feature><Feature recentID="6" name="P777 DFTL_0402FR2R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7125-7135" color="#a020f0" type="standard"/><Q name="note"><V text="P777 DFTL_0402FR2R"/></Q></Feature><Feature recentID="3" name="FTL_0404" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7125-7129" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="3952331" predef="GeneID"/></Q></Feature><Feature recentID="1" name="P778 CkDFTL_0402F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6425-6452" color="#a020f0" type="standard"/><Q name="note"><V text="P778 CkDFTL_0402F"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7853" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL126 pEXDFTL_0402"/></Q></Feature></Features>
  I<?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="P791 DFTL_1548FR2F" sequence="cctttataattttataacaacttaaatctc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6499-6528" boundStrand="0" annealedBases="cctttataattttataacaacttaaatctc" meltingTemperature="51"><Component hybridizedRange="6499-6528" bases="cctttataattttataacaacttaaatctc"/></BindingSite><BindingSite simplified="1" location="6499-6528" boundStrand="0" annealedBases="cctttataattttataacaacttaaatctc" meltingTemperature="51"><Component hybridizedRange="6499-6528" bases="cctttataattttataacaacttaaatctc"/></BindingSite></Primer><Primer recentID="0" name="P792 DFTL_1548FR2R" sequence="tgatactgtagacaacatcatatttaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7096-7123" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="52"><Component hybridizedRange="7096-7123" bases="tgatactgtagacaacatcatatttaag"/></BindingSite><BindingSite simplified="1" location="7096-7123" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="52"><Component hybridizedRange="7096-7123" bases="tgatactgtagacaacatcatatttaag"/></BindingSite></Primer></Primers>
  A<Notes>
<UUID>20cc3d38-202f-4210-9cdc-449084c0e81a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:54:14">2018.6.6</Created>
<LastModified UTC="12:59:54">2018.6.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  E     )  <        <.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAͩVV&`=eiꍚ՛en]R%J!Ueng1Mm(&NemY+EF}{ugUzIXYS8'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ    Y  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ;G<?xml version="1.0"?><Features recycledIDs="4,5,8,9,32,25,20,18" nextValidID="46"><Feature recentID="45" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6550-6566" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="44" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6526-6542" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="43" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="42" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6619-6640" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="6574-6580" color="#ffffff" type="standard"/><Segment range="6581-6598" color="#ffffff" type="standard"/><Segment name="-35" range="6599-6604" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="36" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6928-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="30" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="29" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="28" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="27" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6537-6562" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="26" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6559-6578" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="24" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="23" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="22" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="21" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="19" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="17" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="15" name="P774 DFTL_0402FR1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6107-6141" color="#a020f0" type="standard"/><Q name="note"><V text="P774 DFTL_0402FR1F"/></Q></Feature><Feature recentID="14" name="P775 DFTL_0402FR1R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6465-6499" color="#a020f0" type="standard"/><Q name="note"><V text="P775 DFTL_0402FR1R"/></Q></Feature><Feature recentID="13" name="FTL_0401" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6426" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0401"/></Q><Q name="db_xref"><V text="3952328" predef="GeneID"/></Q></Feature><Feature recentID="12" name="FTL_0401" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6113-6426" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="product"><V text="Sua5_yciO_yrdC family protein"/></Q><Q name="note"><V text="/dnas_title=Sua5_yciO_yrdC family protein"/></Q><Q name="locus_tag"><V text="FTL_0401"/></Q><Q name="db_xref"><V text="89255812" predef="GI"/><V text="3952328" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513174.1"/></Q></Feature><Feature recentID="11" name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6487-6495" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q></Feature><Feature recentID="10" name="FTL_0402" directionality="1" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6603" maxFusedRunOn="6603"><Segment range="6487-6495" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="product"><V text="intracellular septation protein A family&#10;protein"/></Q><Q name="note"><V text="/dnas_title=intracellular septation protein A family&#10;protein"/></Q><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="89255813" predef="GI"/><V text="3952329" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513175.1"/></Q></Feature><Feature recentID="7" name="P776 DFTL_0402FR2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6496-6499" color="#a020f0" type="standard"/><Q name="note"><V text="P776 DFTL_0402FR2F"/></Q></Feature><Feature recentID="6" name="P777 DFTL_0402FR2R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6500-6510" color="#a020f0" type="standard"/><Q name="note"><V text="P777 DFTL_0402FR2R"/></Q></Feature><Feature recentID="3" name="FTL_0404" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6500-6504" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="3952331" predef="GeneID"/></Q></Feature><Feature recentID="2" name="FTL_0404" directionality="2" translateFirstCodonAsMet="1" translationMW="117.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6439" maxFusedRunOn="6439"><Segment range="6500-6504" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="product"><V text="glucose kinase"/></Q><Q name="note"><V text="/dnas_title=glucose kinase"/></Q><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="89255815" predef="GI"/><V text="3952331" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513177.1"/></Q><Q name="EC_number"><V text="2.7.1.2"/></Q></Feature><Feature recentID="1" name="P778 CkDFTL_0402F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6425-6452" color="#a020f0" type="standard"/><Q name="note"><V text="P778 CkDFTL_0402F"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7228" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL126 pEXDFTL_0402"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  A<Notes>
<UUID>20cc3d38-202f-4210-9cdc-449084c0e81a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:54:14">2018.6.6</Created>
<LastModified UTC="12:59:41">2018.6.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  N      /   2         2.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAͩVV&`=eiꍚ՛en]R%J!Ueng1Mm(&NemY+EF}{ugUzIXYS:Zڤ5f&ZMuieUVhu_UYSeWfn*IifHNgBj9櫙t&9ڧBiUYZVg'3>i_i[mʇkE#YYT%z%#^ٙEXႻdu>DWyYs{СeA7ݞYV<t[A`TsezV^ע]Eove{mݯMMJm!nnDk_Z|NŬضN*Vo;uw]O6TSa!9{v;x=8X[\£&ξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	    Y  c  X  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  C<?xml version="1.0"?><Features recycledIDs="32,25,20,18" nextValidID="46"><Feature recentID="45" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7564-7580" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="44" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7540-7556" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="43" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="42" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7633-7654" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="7588-7594" color="#ffffff" type="standard"/><Segment range="7595-7612" color="#ffffff" type="standard"/><Segment name="-35" range="7613-7618" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="36" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7942-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="30" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="29" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="28" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="27" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7551-7576" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="26" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7573-7592" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="24" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="23" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="22" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="21" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="19" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="17" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="15" name="P774 DFTL_0402FR1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6107-6141" color="#a020f0" type="standard"/><Q name="note"><V text="P774 DFTL_0402FR1F"/></Q></Feature><Feature recentID="14" name="P775 DFTL_0402FR1R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6465-6503" color="#a020f0" type="standard"/><Q name="note"><V text="P775 DFTL_0402FR1R"/></Q></Feature><Feature recentID="13" name="FTL_0401" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6426" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0401"/></Q><Q name="db_xref"><V text="3952328" predef="GeneID"/></Q></Feature><Feature recentID="12" name="FTL_0401" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6113-6426" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="product"><V text="Sua5_yciO_yrdC family protein"/></Q><Q name="note"><V text="/dnas_title=Sua5_yciO_yrdC family protein"/></Q><Q name="locus_tag"><V text="FTL_0401"/></Q><Q name="db_xref"><V text="89255812" predef="GI"/><V text="3952328" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513174.1"/></Q></Feature><Feature recentID="11" name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6487-6495" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q></Feature><Feature recentID="10" name="FTL_0402" directionality="1" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6516" maxFusedRunOn="6516"><Segment range="6487-6495" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="product"><V text="intracellular septation protein A family&#10;protein"/></Q><Q name="note"><V text="/dnas_title=intracellular septation protein A family&#10;protein"/></Q><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="89255813" predef="GI"/><V text="3952329" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513175.1"/></Q></Feature><Feature recentID="9" name="FTL_0403" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6581-6832" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0403"/></Q><Q name="db_xref"><V text="3952330" predef="GeneID"/></Q></Feature><Feature recentID="8" name="FTL_0403" directionality="1" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6877" maxFusedRunOn="6877"><Segment range="6581-6832" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V predef="ab initio prediction"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="locus_tag"><V text="FTL_0403"/></Q><Q name="db_xref"><V text="89255814" predef="GI"/><V text="3952330" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513176.1"/></Q></Feature><Feature recentID="7" name="P776 DFTL_0402FR2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6496-6534" color="#a020f0" type="standard"/><Q name="note"><V text="P776 DFTL_0402FR2F"/></Q></Feature><Feature recentID="6" name="P777 DFTL_0402FR2R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7492-7524" color="#a020f0" type="standard"/><Q name="note"><V text="P777 DFTL_0402FR2R"/></Q></Feature><Feature recentID="5" name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6505-6507" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q></Feature><Feature recentID="4" name="FTL_0402" directionality="1" translateFirstCodonAsMet="1" translationMW="131.18" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6516" maxFusedRunOn="6516"><Segment range="6505-6507" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="product"><V text="intracellular septation protein A family&#10;protein"/></Q><Q name="note"><V text="/dnas_title=intracellular septation protein A family&#10;protein"/></Q><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="89255813" predef="GI"/><V text="3952329" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513175.1"/></Q></Feature><Feature recentID="3" name="FTL_0404" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6821-7518" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="3952331" predef="GeneID"/></Q></Feature><Feature recentID="2" name="FTL_0404" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6811" maxFusedRunOn="6811"><Segment range="6821-7518" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="product"><V text="glucose kinase"/></Q><Q name="note"><V text="/dnas_title=glucose kinase"/></Q><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="89255815" predef="GI"/><V text="3952331" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513177.1"/></Q><Q name="EC_number"><V text="2.7.1.2"/></Q></Feature><Feature recentID="1" name="P778 CkDFTL_0402F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6425-6452" color="#a020f0" type="standard"/><Q name="note"><V text="P778 CkDFTL_0402F"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8242" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL126 pEXDFTL_0402"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  A<Notes>
<UUID>20cc3d38-202f-4210-9cdc-449084c0e81a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:54:14">2018.6.6</Created>
<LastModified UTC="12:56:38">2018.6.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  L7zXZ  ִF !   t/s] ˇf{3(1v%f M*	mj)p80*i	NY]QF7&!nxc,@.dβig,ƪv>?
fYfpVVMnI<S4P;5Y˦{
8.W#]jkGYZLvc^aAN`zРу
M˙NkP!Oύjmk.exk}uvdퟃٙ1{>Z4dJ_a$DBw_ѡTu'>oX &/uZ_,kaؗu"}*vEޫ(WwW,"%]=Y	:sҴFV<2	hv=¢giN|dɿR<@n9fU8l-Y]BWqևˉ-#@6UgDKtC'7C6(װyANЏKA|BHcqӥ
>'6T-2@x=$CϹڇ9`8Y\PZ%/Φm8hdRÅ[Z:lfA~]($)S8z+7MM/5A/PҦhe[oTjy\S(v~ጦ{M½W@	ͮ<#N3[&Јcw =
sOvŏ:} D&XF%05Avj15bfK1 $ԖW=x,eq牏@U`\6=M 5>O
|A%T1=	*\c΅%E=^!rc_P$u@W8ըQ6~"}jt3Vsϰ"Ae/}xVeV=?ۄj>czu'/ĩKHNKh& XC:,D'se˘UYX'j-{ dRpLch$MtL溙[iy3s!L՛̧>Ec)~usJ!>/X*Lʢx	KtTό7ZO!f.WJI
w-Folb6\=*CΔ	e&]NД27f7W5ußy.EB~O[z|y%UlAd5ȧ8'ƌ&3oAZ1HLjed(#ViZma81]K%R-Άged͛DذXT^'8xf+Oɻ'"6{)\-čǤ= %P{>"2'.cI<+Z#&~,=pmaiQRu぀Ze]_{S/_2Loq{P?~z;V#,.ƤR6&Qb&$hKY)|4$b]6 M1^O;v9ɜ?18ue5c3ixn`st_1zl
CMkicJz,Ïk6o
ZQ#Õ&1/pen:\E ISFC=['~'mΣ"o"/TطUA%0'8qfemAYS'{cR9&_/kKPq6B rF*HF_Q,{.1>й);K%؝Cn2_^38Ow)ЄV@7d؝!џMH=פf}~3c \+ב6j0čf'yf|vu,	ۖSʋ3YC:YAfN>T!AK-!/uf,煤dwR}[b'TyLad,f*WC5灾 yzìLj:9*Zp8A@)wxɬ.C਄*ZVvJlLp|0pҨ 's3wjGذzj0;>h
Vqt@gMLLCPqUuyʠg3ij#Ւs=FȔ؋%{QNq1"Ѕ>Fl+jH_Ltx%>|_AҦ16Y
?3ˍ{h0+V91ME3'" aV?`KBLO^A)⋲k`[4|٬Xy)ܐ\2xɺfkLisnc~S3r԰dE?sۥ݀|AZ6YviPQg0ų&w]_+JΝۛc8jQMVk9?[:]]%܌鋃d)"6Õ<?}߾r))w[lƧfnH=NQThjp"a?IMA+\ ސ?z73,\@gf;7!Pd]`~vS;mRZpMa	34[.MnbPt]Uc9rY΂@G2L/PX䔨6cDjV#)ߑ?(ef7vW*:5H,8="ke-z3$ |b7V7;0WIjD}=48(v\5TT8Bp>$$Nzz.dOSg!?.@''oeg`yÃjhʰK `rjR"ID=6J4h^y*GgkvBBȧSm~]zYa*WZ:PyLLt%5HMOƱ]Q[n/;'JNIE>DT!^owny֊++c_[>cW\&D2ćv)*:N,o@p   mߥǒ8'  VWn&g    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  =F<?xml version="1.0"?><Features nextValidID="41"><Feature recentID="0" name="DFTL_0545_FR2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6772-7485" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="phosphatidylcholine synthase" directionality="1" translationMW="233.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="253" hitsStopCodon="1"><Segment range="6772-6780" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="KS*"/></Q></Feature><Feature recentID="2" name="FTL_0545" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6772-6780" color="#00e600" type="standard"/></Feature><Feature recentID="3" name="hypothetical protein" directionality="1" translationMW="25545.06" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7518" maxFusedRunOn="7518"><Segment range="6850-7485" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MKVLSQEERQKLFLENIFPYKHKIPRNVYEKQKHYLQIELLKFQKWVKENNKKVLIIFEGRDAAGKGGTIKRMMEHLNPRGAKVIALEKPSEQERNQWYFQRYIEHLPSGGEIVLFDRSWYNRAGVERVMGFCTEREYFLFLEQAPQLEKMLVDSGTMIIKFWFSVSQQEQKNRFAARESHPLKQWKLSPIDKASLDKWDDYTEAKERMFIY"/></Q></Feature><Feature recentID="4" name="FTL_0544" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6850-7485" color="#00e600" type="standard"/></Feature><Feature recentID="5" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6772-7485" color="#000000" type="standard"/></Feature><Feature recentID="6" name="DFTL_0545_FR1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6762" color="#a6acb3" type="standard"/></Feature><Feature recentID="7" name="geranyltranstransferase" directionality="1" translationMW="22172.21" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="96" hitsStopCodon="1"><Segment range="6108-6706" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="TCHIKFNEATAILAGDALQALAFEILQDINYSDIDKLKKINKLFAQYCGASGMVGGQQLDIEGENKKLKIEELQILHINKTAKMFRACIVLPYILSQNQNSQIEDLLVRLSDLIGLCFQIKDDILDVTKTTNELGKTSAKDIDANKSTYVSLMGLKDANKYLLDKKNEITQIITKLKNNNFSSTSLENLIDLVINRNC*"/></Q></Feature><Feature recentID="8" name="FTL_0546" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6706" color="#00e600" type="standard"/></Feature><Feature recentID="9" name="phosphatidylcholine synthase" directionality="1" translationMW="376.49" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6780" maxFusedRunOn="6780"><Segment range="6754-6762" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MQV"/></Q></Feature><Feature recentID="10" name="FTL_0545" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6754-6762" color="#00e600" type="standard"/></Feature><Feature recentID="11" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6762" color="#000000" type="standard"/></Feature><Feature recentID="12" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7536-7552" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7512-7528" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7605-7626" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="7560-7566" color="#ffffff" type="standard"/><Segment range="7567-7584" color="#ffffff" type="standard"/><Segment name="-35" range="7585-7590" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="21" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7914-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature recentID="24" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MNIKKFAKQATVLTFTTALLAGGATQAFA,KETNQKPYKETYGISHITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK*"/></Q></Feature><Feature recentID="25" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="26" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="27" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="28" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="29" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7523-7548" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="30" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7545-7564" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="31" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="32" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="33" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="34" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="35" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="36" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="37" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="38" name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6763-6767" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0402"/></Q></Feature><Feature recentID="39" name="FTL_0404" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7486-7490" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952331" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0404"/></Q></Feature><Feature recentID="40" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8214" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL126 pEXDFTL_0402"/></Q></Feature></Features>
  -<?xml version="1.0"?><Primers nextValidID="5"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="P800 DFTL_0545_int1" sequence="actagtcttgaaaatctcattgatttag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6658-6685" boundStrand="0" annealedBases="actagtcttgaaaatctcattgatttag" meltingTemperature="54"><Component hybridizedRange="6658-6685" bases="actagtcttgaaaatctcattgatttag"/></BindingSite><BindingSite simplified="1" location="6658-6685" boundStrand="0" annealedBases="actagtcttgaaaatctcattgatttag" meltingTemperature="54"><Component hybridizedRange="6658-6685" bases="actagtcttgaaaatctcattgatttag"/></BindingSite></Primer><Primer recentID="1" name="P798 DFTL_0545FR2F" sequence="GCGGCCGCTaaaagttaatctgtttacctcttaatcac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6762-6799" boundStrand="0" annealedBases="GCGGCCGCTaaaagttaatctgtttacctcttaatcac" meltingTemperature="64"><Component hybridizedRange="6762-6799" bases="GCGGCCGCTaaaagttaatctgtttacctcttaatcac"/></BindingSite><BindingSite simplified="1" location="6762-6799" boundStrand="0" annealedBases="GCGGCCGCTaaaagttaatctgtttacctcttaatcac" meltingTemperature="64"><Component hybridizedRange="6762-6799" bases="GCGGCCGCTaaaagttaatctgtttacctcttaatcac"/></BindingSite></Primer><Primer recentID="2" name="P799 DFTL_0545FR2R" sequence="GGTACCatatataaacatcctttctttagcttcag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7456-7490" boundStrand="1" annealedBases="GGTACCatatataaacatcctttctttagcttcag" meltingTemperature="59"><Component hybridizedRange="7456-7490" bases="GGTACCatatataaacatcctttctttagcttcag"/></BindingSite><BindingSite simplified="1" location="7456-7490" boundStrand="1" annealedBases="GGTACCatatataaacatcctttctttagcttcag" meltingTemperature="59"><Component hybridizedRange="7456-7490" bases="GGTACCatatataaacatcctttctttagcttcag"/></BindingSite></Primer><Primer recentID="3" name="P797 DFTL_0545FR1R" sequence="GCGGCCGCtacttgcattttaatattaacttttaaataaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6730-6769" boundStrand="1" annealedBases="GCGGCCGCtacttgcattttaatattaacttttaaataaa" meltingTemperature="61"><Component hybridizedRange="6730-6769" bases="GCGGCCGCtacttgcattttaatattaacttttaaataaa"/></BindingSite><BindingSite simplified="1" location="6730-6769" boundStrand="1" annealedBases="GCGGCCGCtacttgcattttaatattaacttttaaataaa" meltingTemperature="61"><Component hybridizedRange="6730-6769" bases="GCGGCCGCtacttgcattttaatattaacttttaaataaa"/></BindingSite></Primer><Primer recentID="4" name="P796 DFTL_0545FR1F" sequence="GGATCCcaacttgtcatatcaaatttaatgaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6101-6134" boundStrand="0" annealedBases="GGATCCcaacttgtcatatcaaatttaatgaagc" meltingTemperature="60"><Component hybridizedRange="6101-6134" bases="GGATCCcaacttgtcatatcaaatttaatgaagc"/></BindingSite><BindingSite simplified="1" location="6101-6134" boundStrand="0" annealedBases="GGATCCcaacttgtcatatcaaatttaatgaagc" meltingTemperature="60"><Component hybridizedRange="6101-6134" bases="GGATCCcaacttgtcatatcaaatttaatgaagc"/></BindingSite></Primer></Primers>
  @<Notes>
<UUID>20cc3d38-202f-4210-9cdc-449084c0e81a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:54:14">2018.6.6</Created>
<LastModified UTC="14:8:24">2018.6.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         Unsaved Enzyme Set                                                                                           d     CHO  d              E              Y? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   <?xml version="1.0"?><CustomEnzymeSets><CustomEnzymeSet type="2" name="Unsaved Enzyme Set" enzymeNames="BamHI KpnI NotI"/></CustomEnzymeSets>
