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h38\&0l>  b!	
r{8eW m E
8o	n
ER1Z  CZKO
59* d   _tXdhvDhO~f! 
 a' J  2 H+	
/
@=u?|<;Ck" / E   Lw	
^

J`p.uT(  l7		
dRt[cMc"G_tV 2  RRj
M>*NV48G# ZvfH?
Sy..z 7dtg)~+    !8FiN	gq5[zFek= l `n	DNc}	4!   1kK6i	


-BbU8  u>h5m	Rpy-EAdyc(aj}<\   Nstst#0189l				uvwx$,MNO@4YZcwx&
%&'F u$2{l:XH	P		"%Pu~5[o'BFf	u'o)   Oy,b&t+L			
 m_q:ms+% A%	Mm;	##vN5T`Xg
?5 v3#;U	I	Q		r]6\HQ\Cjv(+ 6|4Y
_Bp}( .~Mp4 {;I}x
9YiQM&Q-GIyT JU\c&,A
!rt) <   -;]2M	
tn`	L~	=]n% x Pz'u
#N

iRZI-+;wK   e			-#'_p,  BhN>D^
_6h@$ hO
a BRq(			f$I	>g+% R <	

$"0	OkUaY.X' G'&9d3njo	
R

5k7w(FA kR		M tXs-]8 ?w4$rGVcJ		v
	s*!]mFI];D[kn:) V<PE	4	:`^GR^w)$ b0	mz7Ob,2T`@ 
ICZ	 	c


(C gq~qq-$ B mx$				Sz$x18NQ<@  _75O<e	6	@	c	

ntU`<bi|2mISAB!/ .X}6df )N$  # y
[]b[Nt>o66 p<H~>			Y
 d:FZjR''>TW{.J9<Uq	   ,z^$&8RV<`'Hz%  $|!NT{JGzj. <yw  | 5]0'-		=}  KEB	#J Udz	
-|"} ?QVtlCsVu = `  
'u	
>o$
.472%  dy.<^03N	

wpaZ
@^V 2	a			&YrMo<   	ul> ) 6 (u$h

+j4h0.<$)bYH y  #O
:Jn37  {vI[,xLn QZnSWDQy  K ]o$sd9dC}   Er f	s
3q(!`MB   ,u			.}q  d'gL-q C	`3/ GeGZj7&R  8 o     ci-?&EA @i( + r ;*OS	8
H
'_K^3A&  iPr)	j
8I3P<n 2ds , j  q![S	9		g+YL%J
dhm	9  *_b1:@ENh	

x^iDV*LY+?, =.Cx				
1%"J G-T%;E6Xl>b[ik% S =	
e
VUo1jPlQ;  KX5






*'/=s8UY|?S0"sVZ/bsj "	 
?



Ici#
]2Y$  w4S


-l|)(CB(Rd` "  Heo\	p
6X058xq \  'jk
~lpCHPn\,  (:{pq	

[5Gu U lS		
`I.  C	2N!Vn 0WguY D   c;MtS^  YE	
M-|f3@ x+z%sWK		Q
!ot+DL^n%Elr$t&;6 @~5LH|w

XA"4|GPSiH->S# ; w :%d	M

svJ^<\ o  Q N8=QnQ
C/6f*" ja	s lE:]_9$  WB	
		5	?		
;alHR@RA 7P,-   "  oSF			%U_&Sz58x;vp  { 4,{k<B|U _  x&/t1	=Xq-3;U~6 Ymg
*3VCPam  c\nc8pc BA|   b,7
@22.;  i  )p0?ZDg	8	


7w]XhmKC$UKXr!4  J!		d





)H:bDWkrP1r-  iz[

}ZW/o~4~4' C B	_1bH:fU9 X}*9y	L


r
n@{3u{O$2Rhq#=%1 ! nM7R						
$.r5?%@Dy9O\7u   l%W)jAT:l}{I    (ao+>yf7	




|v?Va.n}W3VJjB1&7 86Q	A	
q	a#Gm?-qT>2zCN#pt     /`P=x23vw 	




opKLMuF
-=>cdjklm*+,_nopU=1"yz06 04ax	d			

,1 BNe[`xF#+8h{dJ  		2,9MO^0 }/h Y!5b	

!CO_fuaG$!l*|' f 1y

)7F\ey.i & *~@Y	e	-IgKQe8>Kl  	A
z
8u? W_ 5[mnc\O-_pJ  	\^5:7P[] $ V	3KebwZ[NB0 C0	#
-
Z

;P=UvH(?" =r$ qi!ZI6?	
eg|+_{2 f="c<&		"D[k`Sg (b%:Um/}:V[! S~p		!GXK}Fw
   ;c	


%AWhz= ( 2_z}	*SajF4*  g  -{g>


'8DkG] )K/SI{6 
:$		9;%fYe  AUZ	/x9m   +D}O){k%JF% %\K
.'R=f?g "|Hf		hLRzLY 6Y^
1
;
B
>1nE  .1-		

z=!  Y  (			?>9J@#Xm " }  &@q.V_U
{
wYGQv~`f L 64n6F	qEi Lj	B]KP!~+aK) %\vyoCd<x`q  OC$f
.0o   +~^ WJ	 .A+Q*0 V	

]_z}~k;f8\H  Ye{		!
+
#6D 	MWWt\			)
u&/x RK^	
m[!;Dt\*v%   @4f1EyOz3;COTbx % % #t+wL(QcDuW	 - > 1}	$

'Vw
LU^bx0pz@#O>3D' G a a (
[
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27A]%/5DJV "   D^Y~Q &N8( . 1  Qv		.

?<>{LIs)U:Zbs  ejJ_Mh

fxbs3   r"/=74O	

tbvA`
|  z	8hq[,_Wx}  T["1@
,*} gFg'			!^H=W! >Uqb3	u
l)FNS\- -N~#d	b			~
'ms5Thcf#w0p~;"  MG =yX
Z#E\aZ&-;=s;Vn  T	" `			vo#kY?~ ( 5  tHm2Gx  Jq+t	r
rL * 7  )Rd%G
iJ]%Q%  <-^Mw		i
O*01)*cI * W


&,Tnhr5TXi{>R/!=Zahi$   v
kBGO'p Qct 	  
 v ${~	CKeGs `m	P
TQk4e8 ^ z  3w.s+;bUo{5&  h  h	

69\ 3TJ O   |)K
?|6$z : '.nw1	


0J"El,)^*70yMo!  |)g? 
(^EHJL-k7= Ulm	vYZ^IAV\& BRo;%	f<T9|Evz   =9		:gXJR5d C[	&QEXf   %?-U^	
FP~  $Bi	p *e	D/n V 
y*je)  "L*sJ[	%P0t :y:Nw &  P|} z&K@T]rTw%YaNIC  !g)|	 ,E~LTF}	t
l4e,~rG": ' 4  s*s	j' ]VLv	N/SNIgPhs u v-lr	~BTr$  &f	

/!]+(6>  T<	P8XI@[  >#~ITh		
i)M. S\$i{ e	O7MSOZr }g	(hMN  0  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                      *                                                      
      	                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
                                                                                                                            	                                                                                                                                         -               	                                                                     !                                                                        
   	                     	   
               	            (      	               	                                          	                     
                                       )   )      (   #   	      $            ~      "   f   a   +         9   m                                                                                                                                                                           *                          
  	                                                                                                                                                                                                                                                                  
                                                  	                                                       -     	                         !                          
 	       	 
     	    (  	     	              	       
             ) )  ( # 	  $    ~  " f a +   9 m   C  e      +  [  
u I	*r)*w"/1 X#q
^OR4ccccXvS 'OR[dS<;)lB


 5I&Zc eDkDNd4	 y	D9(y	3( @r@y*	j
wQ{3^'L ry	C|o
o[Fo
lQ<o
Fbo	\r.	$^ sT5ruCw1ArW	@ 6<d,}|S\O} $]EO<X  		o
o[Fof&		=J54 $QF
yKmqu2EOSDX\h
t	,"xh6t	m#pFD .  
I kOLdn
tk(o

Ft3 g  f"\cK&			!eUSI Am$EO .
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o1d^iixb6 #zii hp YZ5		8	
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consecutiveTranslationNumbering="1"><Segment range="6465-6499" color="#a020f0" type="standard"/><Q name="note"><V text="P775 DFTL_0402FR1R"/></Q></Feature><Feature recentID="13" name="FTL_0401" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6426" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0401"/></Q><Q name="db_xref"><V text="3952328" predef="GeneID"/></Q></Feature><Feature recentID="12" name="FTL_0401" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6113-6426" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="product"><V text="Sua5_yciO_yrdC family protein"/></Q><Q name="note"><V text="/dnas_title=Sua5_yciO_yrdC family protein"/></Q><Q name="locus_tag"><V text="FTL_0401"/></Q><Q name="db_xref"><V text="89255812" predef="GI"/><V text="3952328" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513174.1"/></Q></Feature><Feature recentID="11" name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6487-6495" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q></Feature><Feature recentID="10" name="FTL_0402" directionality="1" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6516" maxFusedRunOn="6516"><Segment range="6487-6495" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="product"><V text="intracellular septation protein A family&#10;protein"/></Q><Q name="note"><V text="/dnas_title=intracellular septation protein A family&#10;protein"/></Q><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="89255813" predef="GI"/><V text="3952329" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513175.1"/></Q></Feature><Feature recentID="7" name="P776 DFTL_0402FR2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6496-6499" color="#a020f0" type="standard"/><Q name="note"><V text="P776 DFTL_0402FR2F"/></Q></Feature><Feature recentID="6" name="P777 DFTL_0402FR2R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7125-7135" color="#a020f0" type="standard"/><Q name="note"><V text="P777 DFTL_0402FR2R"/></Q></Feature><Feature recentID="3" name="FTL_0404" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7125-7129" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="3952331" predef="GeneID"/></Q></Feature><Feature recentID="1" name="P778 CkDFTL_0402F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6425-6452" color="#a020f0" type="standard"/><Q name="note"><V text="P778 CkDFTL_0402F"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7853" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL126 pEXDFTL_0402"/></Q></Feature></Features>
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  A<Notes>
<UUID>20cc3d38-202f-4210-9cdc-449084c0e81a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:54:14">2018.6.6</Created>
<LastModified UTC="12:59:54">2018.6.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAͩVV&`=eiꍚ՛en]R%J!Ueng1Mm(&NemY+EF}{ugUzIXYS8'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ    Y  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="44" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6526-6542" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="43" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="42" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" 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type="standard"/><Segment range="6581-6598" color="#ffffff" type="standard"/><Segment name="-35" range="6599-6604" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="36" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6928-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="30" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="29" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="28" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="27" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6537-6562" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="26" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6559-6578" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="24" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="23" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="22" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="21" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="19" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="17" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="15" name="P774 DFTL_0402FR1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6107-6141" color="#a020f0" type="standard"/><Q name="note"><V text="P774 DFTL_0402FR1F"/></Q></Feature><Feature recentID="14" name="P775 DFTL_0402FR1R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6465-6499" color="#a020f0" type="standard"/><Q name="note"><V text="P775 DFTL_0402FR1R"/></Q></Feature><Feature recentID="13" name="FTL_0401" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6426" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0401"/></Q><Q name="db_xref"><V text="3952328" predef="GeneID"/></Q></Feature><Feature recentID="12" name="FTL_0401" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6113-6426" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="product"><V text="Sua5_yciO_yrdC family protein"/></Q><Q name="note"><V text="/dnas_title=Sua5_yciO_yrdC family protein"/></Q><Q name="locus_tag"><V text="FTL_0401"/></Q><Q name="db_xref"><V text="89255812" predef="GI"/><V text="3952328" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513174.1"/></Q></Feature><Feature recentID="11" name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6487-6495" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q></Feature><Feature recentID="10" name="FTL_0402" directionality="1" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6603" maxFusedRunOn="6603"><Segment range="6487-6495" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="product"><V text="intracellular septation protein A family&#10;protein"/></Q><Q name="note"><V text="/dnas_title=intracellular septation protein A family&#10;protein"/></Q><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="89255813" predef="GI"/><V text="3952329" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513175.1"/></Q></Feature><Feature recentID="7" name="P776 DFTL_0402FR2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6496-6499" color="#a020f0" type="standard"/><Q name="note"><V text="P776 DFTL_0402FR2F"/></Q></Feature><Feature recentID="6" name="P777 DFTL_0402FR2R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6500-6510" color="#a020f0" type="standard"/><Q name="note"><V text="P777 DFTL_0402FR2R"/></Q></Feature><Feature recentID="3" name="FTL_0404" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6500-6504" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="3952331" predef="GeneID"/></Q></Feature><Feature recentID="2" name="FTL_0404" directionality="2" translateFirstCodonAsMet="1" translationMW="117.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6439" maxFusedRunOn="6439"><Segment range="6500-6504" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="product"><V text="glucose kinase"/></Q><Q name="note"><V text="/dnas_title=glucose kinase"/></Q><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="89255815" predef="GI"/><V text="3952331" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513177.1"/></Q><Q name="EC_number"><V text="2.7.1.2"/></Q></Feature><Feature recentID="1" name="P778 CkDFTL_0402F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6425-6452" color="#a020f0" type="standard"/><Q name="note"><V text="P778 CkDFTL_0402F"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7228" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL126 pEXDFTL_0402"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  A<Notes>
<UUID>20cc3d38-202f-4210-9cdc-449084c0e81a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:54:14">2018.6.6</Created>
<LastModified UTC="12:59:41">2018.6.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  N      /   2         2.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
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ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
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ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	    Y  c  X  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="44" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7540-7556" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="43" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="42" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7633-7654" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="7588-7594" color="#ffffff" type="standard"/><Segment range="7595-7612" color="#ffffff" type="standard"/><Segment name="-35" range="7613-7618" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="36" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7942-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="30" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="29" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="28" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="27" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7551-7576" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="26" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7573-7592" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="24" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="23" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="22" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="21" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="19" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="17" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="15" name="P774 DFTL_0402FR1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6107-6141" color="#a020f0" type="standard"/><Q name="note"><V text="P774 DFTL_0402FR1F"/></Q></Feature><Feature recentID="14" name="P775 DFTL_0402FR1R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6465-6503" color="#a020f0" type="standard"/><Q name="note"><V text="P775 DFTL_0402FR1R"/></Q></Feature><Feature recentID="13" name="FTL_0401" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6426" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0401"/></Q><Q name="db_xref"><V text="3952328" predef="GeneID"/></Q></Feature><Feature recentID="12" name="FTL_0401" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6113-6426" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="product"><V text="Sua5_yciO_yrdC family protein"/></Q><Q name="note"><V text="/dnas_title=Sua5_yciO_yrdC family protein"/></Q><Q name="locus_tag"><V text="FTL_0401"/></Q><Q name="db_xref"><V text="89255812" predef="GI"/><V text="3952328" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513174.1"/></Q></Feature><Feature recentID="11" name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6487-6495" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q></Feature><Feature recentID="10" name="FTL_0402" directionality="1" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6516" maxFusedRunOn="6516"><Segment range="6487-6495" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="product"><V text="intracellular septation protein A family&#10;protein"/></Q><Q name="note"><V text="/dnas_title=intracellular septation protein A family&#10;protein"/></Q><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="89255813" predef="GI"/><V text="3952329" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513175.1"/></Q></Feature><Feature recentID="9" name="FTL_0403" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6581-6832" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0403"/></Q><Q name="db_xref"><V text="3952330" predef="GeneID"/></Q></Feature><Feature recentID="8" name="FTL_0403" directionality="1" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6877" maxFusedRunOn="6877"><Segment range="6581-6832" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V predef="ab initio prediction"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="locus_tag"><V text="FTL_0403"/></Q><Q name="db_xref"><V text="89255814" predef="GI"/><V text="3952330" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513176.1"/></Q></Feature><Feature recentID="7" name="P776 DFTL_0402FR2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6496-6534" color="#a020f0" type="standard"/><Q name="note"><V text="P776 DFTL_0402FR2F"/></Q></Feature><Feature recentID="6" name="P777 DFTL_0402FR2R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7492-7524" color="#a020f0" type="standard"/><Q name="note"><V text="P777 DFTL_0402FR2R"/></Q></Feature><Feature recentID="5" name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6505-6507" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q></Feature><Feature recentID="4" name="FTL_0402" directionality="1" translateFirstCodonAsMet="1" translationMW="131.18" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6516" maxFusedRunOn="6516"><Segment range="6505-6507" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="product"><V text="intracellular septation protein A family&#10;protein"/></Q><Q name="note"><V text="/dnas_title=intracellular septation protein A family&#10;protein"/></Q><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="89255813" predef="GI"/><V text="3952329" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513175.1"/></Q></Feature><Feature recentID="3" name="FTL_0404" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6821-7518" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="3952331" predef="GeneID"/></Q></Feature><Feature recentID="2" name="FTL_0404" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6811" maxFusedRunOn="6811"><Segment range="6821-7518" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="product"><V text="glucose kinase"/></Q><Q name="note"><V text="/dnas_title=glucose kinase"/></Q><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="89255815" predef="GI"/><V text="3952331" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513177.1"/></Q><Q name="EC_number"><V text="2.7.1.2"/></Q></Feature><Feature recentID="1" name="P778 CkDFTL_0402F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6425-6452" color="#a020f0" type="standard"/><Q name="note"><V text="P778 CkDFTL_0402F"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8242" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL126 pEXDFTL_0402"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  A<Notes>
<UUID>20cc3d38-202f-4210-9cdc-449084c0e81a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:54:14">2018.6.6</Created>
<LastModified UTC="12:56:38">2018.6.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  M               .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAͩVV&`=eiꍚ՛en]R%J!Ueng1Mm(&NemY+EF}{ugUzIXYS;eeץn:XU^EVdXxv˖CgXg`\%:VeU!t +GN^aZ]?RT%i`=M"9F_JV,aXd^fZSR4Fܕ
}TU}Y(m	;[b8!Z)ֽvq<R%O#億Z2Wial=s0C
;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%    Y  c    !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="44" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7151-7167" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="43" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="42" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7244-7265" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="7199-7205" color="#ffffff" type="standard"/><Segment range="7206-7223" color="#ffffff" type="standard"/><Segment name="-35" range="7224-7229" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="36" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7553-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="30" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="29" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="28" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="27" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7162-7187" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="26" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7184-7203" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="24" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="23" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="22" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="21" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="19" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="17" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="15" name="P774 DFTL_0402FR1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6107-6141" color="#a020f0" type="standard"/><Q name="note"><V text="P774 DFTL_0402FR1F"/></Q></Feature><Feature recentID="14" name="P775 DFTL_0402FR1R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6465-6499" color="#a020f0" type="standard"/><Q name="note"><V text="P775 DFTL_0402FR1R"/></Q></Feature><Feature recentID="13" name="FTL_0401" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6426" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0401"/></Q><Q name="db_xref"><V text="3952328" predef="GeneID"/></Q></Feature><Feature recentID="12" name="FTL_0401" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6113-6426" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="product"><V text="Sua5_yciO_yrdC family protein"/></Q><Q name="note"><V text="/dnas_title=Sua5_yciO_yrdC family protein"/></Q><Q name="locus_tag"><V text="FTL_0401"/></Q><Q name="db_xref"><V text="89255812" predef="GI"/><V text="3952328" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513174.1"/></Q></Feature><Feature recentID="11" name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6487-6495" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q></Feature><Feature recentID="10" name="FTL_0402" directionality="1" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6516" maxFusedRunOn="6516"><Segment range="6487-6495" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="product"><V text="intracellular septation protein A family&#10;protein"/></Q><Q name="note"><V text="/dnas_title=intracellular septation protein A family&#10;protein"/></Q><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="89255813" predef="GI"/><V text="3952329" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513175.1"/></Q></Feature><Feature recentID="7" name="P776 DFTL_0402FR2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6496-6499" color="#a020f0" type="standard"/><Q name="note"><V text="P776 DFTL_0402FR2F"/></Q></Feature><Feature recentID="6" name="P777 DFTL_0402FR2R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7125-7135" color="#a020f0" type="standard"/><Q name="note"><V text="P777 DFTL_0402FR2R"/></Q></Feature><Feature recentID="3" name="FTL_0404" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7125-7129" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="3952331" predef="GeneID"/></Q></Feature><Feature recentID="1" name="P778 CkDFTL_0402F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6425-6452" color="#a020f0" type="standard"/><Q name="note"><V text="P778 CkDFTL_0402F"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7853" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL126 pEXDFTL_0402"/></Q></Feature></Features>
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  A<Notes>
<UUID>20cc3d38-202f-4210-9cdc-449084c0e81a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:54:14">2018.6.6</Created>
<LastModified UTC="12:59:54">2018.6.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAͩVV&`=eiꍚ՛en]R%J!Ueng1Mm(&NemY+EF}{ugUzIXYS8'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ    Y  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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type="standard"/><Segment range="6581-6598" color="#ffffff" type="standard"/><Segment name="-35" range="6599-6604" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="36" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6928-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="30" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="29" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="28" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="27" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6537-6562" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="26" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6559-6578" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="24" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="23" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="22" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="21" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="19" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="17" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="15" name="P774 DFTL_0402FR1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6107-6141" color="#a020f0" type="standard"/><Q name="note"><V text="P774 DFTL_0402FR1F"/></Q></Feature><Feature recentID="14" name="P775 DFTL_0402FR1R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6465-6499" color="#a020f0" type="standard"/><Q name="note"><V text="P775 DFTL_0402FR1R"/></Q></Feature><Feature recentID="13" name="FTL_0401" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6426" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0401"/></Q><Q name="db_xref"><V text="3952328" predef="GeneID"/></Q></Feature><Feature recentID="12" name="FTL_0401" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6113-6426" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="product"><V text="Sua5_yciO_yrdC family protein"/></Q><Q name="note"><V text="/dnas_title=Sua5_yciO_yrdC family protein"/></Q><Q name="locus_tag"><V text="FTL_0401"/></Q><Q name="db_xref"><V text="89255812" predef="GI"/><V text="3952328" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513174.1"/></Q></Feature><Feature recentID="11" name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6487-6495" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q></Feature><Feature recentID="10" name="FTL_0402" directionality="1" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6603" maxFusedRunOn="6603"><Segment range="6487-6495" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="product"><V text="intracellular septation protein A family&#10;protein"/></Q><Q name="note"><V text="/dnas_title=intracellular septation protein A family&#10;protein"/></Q><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="89255813" predef="GI"/><V text="3952329" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513175.1"/></Q></Feature><Feature recentID="7" name="P776 DFTL_0402FR2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6496-6499" color="#a020f0" type="standard"/><Q name="note"><V text="P776 DFTL_0402FR2F"/></Q></Feature><Feature recentID="6" name="P777 DFTL_0402FR2R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6500-6510" color="#a020f0" type="standard"/><Q name="note"><V text="P777 DFTL_0402FR2R"/></Q></Feature><Feature recentID="3" name="FTL_0404" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6500-6504" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="3952331" predef="GeneID"/></Q></Feature><Feature recentID="2" name="FTL_0404" directionality="2" translateFirstCodonAsMet="1" translationMW="117.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6439" maxFusedRunOn="6439"><Segment range="6500-6504" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="product"><V text="glucose kinase"/></Q><Q name="note"><V text="/dnas_title=glucose kinase"/></Q><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="89255815" predef="GI"/><V text="3952331" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513177.1"/></Q><Q name="EC_number"><V text="2.7.1.2"/></Q></Feature><Feature recentID="1" name="P778 CkDFTL_0402F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6425-6452" color="#a020f0" type="standard"/><Q name="note"><V text="P778 CkDFTL_0402F"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7228" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL126 pEXDFTL_0402"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  A<Notes>
<UUID>20cc3d38-202f-4210-9cdc-449084c0e81a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:54:14">2018.6.6</Created>
<LastModified UTC="12:59:41">2018.6.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  N      /   2         2.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
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ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
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ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	    Y  c  X  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="44" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7540-7556" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="43" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="42" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7633-7654" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="7588-7594" color="#ffffff" type="standard"/><Segment range="7595-7612" color="#ffffff" type="standard"/><Segment name="-35" range="7613-7618" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="36" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7942-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="30" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="29" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="28" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="27" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7551-7576" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="26" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7573-7592" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="24" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="23" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="22" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="21" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="19" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="17" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="15" name="P774 DFTL_0402FR1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6107-6141" color="#a020f0" type="standard"/><Q name="note"><V text="P774 DFTL_0402FR1F"/></Q></Feature><Feature recentID="14" name="P775 DFTL_0402FR1R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6465-6503" color="#a020f0" type="standard"/><Q name="note"><V text="P775 DFTL_0402FR1R"/></Q></Feature><Feature recentID="13" name="FTL_0401" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6426" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0401"/></Q><Q name="db_xref"><V text="3952328" predef="GeneID"/></Q></Feature><Feature recentID="12" name="FTL_0401" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6113-6426" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="product"><V text="Sua5_yciO_yrdC family protein"/></Q><Q name="note"><V text="/dnas_title=Sua5_yciO_yrdC family protein"/></Q><Q name="locus_tag"><V text="FTL_0401"/></Q><Q name="db_xref"><V text="89255812" predef="GI"/><V text="3952328" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513174.1"/></Q></Feature><Feature recentID="11" name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6487-6495" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q></Feature><Feature recentID="10" name="FTL_0402" directionality="1" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6516" maxFusedRunOn="6516"><Segment range="6487-6495" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="product"><V text="intracellular septation protein A family&#10;protein"/></Q><Q name="note"><V text="/dnas_title=intracellular septation protein A family&#10;protein"/></Q><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="89255813" predef="GI"/><V text="3952329" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513175.1"/></Q></Feature><Feature recentID="9" name="FTL_0403" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6581-6832" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0403"/></Q><Q name="db_xref"><V text="3952330" predef="GeneID"/></Q></Feature><Feature recentID="8" name="FTL_0403" directionality="1" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6877" maxFusedRunOn="6877"><Segment range="6581-6832" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V predef="ab initio prediction"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="locus_tag"><V text="FTL_0403"/></Q><Q name="db_xref"><V text="89255814" predef="GI"/><V text="3952330" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513176.1"/></Q></Feature><Feature recentID="7" name="P776 DFTL_0402FR2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6496-6534" color="#a020f0" type="standard"/><Q name="note"><V text="P776 DFTL_0402FR2F"/></Q></Feature><Feature recentID="6" name="P777 DFTL_0402FR2R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7492-7524" color="#a020f0" type="standard"/><Q name="note"><V text="P777 DFTL_0402FR2R"/></Q></Feature><Feature recentID="5" name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6505-6507" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q></Feature><Feature recentID="4" name="FTL_0402" directionality="1" translateFirstCodonAsMet="1" translationMW="131.18" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6516" maxFusedRunOn="6516"><Segment range="6505-6507" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="product"><V text="intracellular septation protein A family&#10;protein"/></Q><Q name="note"><V text="/dnas_title=intracellular septation protein A family&#10;protein"/></Q><Q name="locus_tag"><V text="FTL_0402"/></Q><Q name="db_xref"><V text="89255813" predef="GI"/><V text="3952329" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513175.1"/></Q></Feature><Feature recentID="3" name="FTL_0404" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6821-7518" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="3952331" predef="GeneID"/></Q></Feature><Feature recentID="2" name="FTL_0404" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6811" maxFusedRunOn="6811"><Segment range="6821-7518" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="product"><V text="glucose kinase"/></Q><Q name="note"><V text="/dnas_title=glucose kinase"/></Q><Q name="locus_tag"><V text="FTL_0404"/></Q><Q name="db_xref"><V text="89255815" predef="GI"/><V text="3952331" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513177.1"/></Q><Q name="EC_number"><V text="2.7.1.2"/></Q></Feature><Feature recentID="1" name="P778 CkDFTL_0402F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6425-6452" color="#a020f0" type="standard"/><Q name="note"><V text="P778 CkDFTL_0402F"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8242" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL126 pEXDFTL_0402"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  A<Notes>
<UUID>20cc3d38-202f-4210-9cdc-449084c0e81a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:54:14">2018.6.6</Created>
<LastModified UTC="12:56:38">2018.6.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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^ɩM0zg[tG(ݦrmlFsTع9B7I8D7Es@^P$Qi8kO(fW:,3͒d- g{[+r\^zL8yٖ}k<߷[?:%' _@A)J䅊RQ1QZ(h (Dbܘ+?Գ7,RP(&)%(C\XtdSxnҍՍ)$!6 XM?ͫoIdv|֋q+/%Ťy[	Ge Gd}nj<DnЄz]/<ޫM볽(/ck3x6y(w
%L~}[:UDG,>  r.Q,}  qF!g    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ;<?xml version="1.0"?><Features nextValidID="39"><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8217" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL126 pEXDFTL_0402"/></Q></Feature><Feature recentID="1" name="FTL_0404" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7489-7493" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952331" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0404"/></Q></Feature><Feature recentID="2" name="FTL_0402" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6855-6859" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952329" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0402"/></Q></Feature><Feature recentID="3" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="4" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="5" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="6" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="7" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="8" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="9" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="10" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7548-7567" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="11" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7526-7551" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="12" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="13" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="14" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="15" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="16" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MNIKKFAKQATVLTFTTALLAGGATQAFA,KETNQKPYKETYGISHITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK*"/></Q></Feature><Feature recentID="17" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature recentID="18" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7917-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="21" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="7563-7569" color="#ffffff" type="standard"/><Segment range="7570-7587" color="#ffffff" type="standard"/><Segment name="-35" range="7588-7593" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7608-7629" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7515-7531" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7539-7555" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6864-7488" color="#000000" type="standard"/></Feature><Feature recentID="30" name="hypothetical protein" directionality="1" translationMW="228.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="265" hitsStopCodon="1"><Segment range="6864-6872" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="PL*"/></Q></Feature><Feature recentID="31" name="FTL_1549" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6906-7488" color="#00e600" type="standard"/></Feature><Feature recentID="32" name="tRNA-dihydrouridine synthase A" directionality="1" translationMW="22327.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7592" maxFusedRunOn="7592"><Segment range="6906-7488" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MKITTRKICIAPMLDWTDRHYRYMMRLITRHTMLYTEMVTLNAILHGNKEFLLKYNPQENPVTLQLGGCVPKDFISCGKLAEELDYNEININVGCPSERVKKGNFGLSLMAEPELVADCVKAMKDNLNIPISVKCRIGYDHNDSYEELYNFVDKQVQAGIDYLCIHARKGWLSGLSPKENRTIPELKYDVVYSI"/></Q></Feature><Feature recentID="33" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6864-7488" color="#a6acb3" type="standard"/></Feature><Feature recentID="34" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6854" color="#000000" type="standard"/></Feature><Feature recentID="35" name="FTL_1547" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6664" color="#00e600" type="standard"/></Feature><Feature recentID="36" name="DNA gyrase subunit B" directionality="2" translationMW="20114.97" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="5858" maxFusedRunOn="5858"><Segment range="6108-6664" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSENKAYDSSSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVVDNAIDEALAGYCDDIKVIINKDGSVSVSDNGRGIPTDIHKEEGRSAAEVILTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEQLKLTIYRNGIEHYQEYVHGVPQFPLKEVGSTDKTGTLITFKPSKETFSFVDFDY"/></Q></Feature><Feature recentID="37" name="FTL_1545 hypothetical protein" directionality="1" translationMW="387.52" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6872" maxFusedRunOn="6872"><Segment range="6846-6854" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="LKK"/></Q></Feature><Feature recentID="38" name="DFTL_1548_FR1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6854" color="#a6acb3" type="standard"/></Feature></Features>
  p<?xml version="1.0"?><Primers nextValidID="5"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="P793 DFTL_1548_int1" sequence="atagcattatcaacaacctcaaatacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6515-6541" boundStrand="0" annealedBases="atagcattatcaacaacctcaaatacc" meltingTemperature="56"><Component hybridizedRange="6515-6541" bases="atagcattatcaacaacctcaaatacc"/></BindingSite><BindingSite simplified="1" location="6515-6541" boundStrand="0" annealedBases="atagcattatcaacaacctcaaatacc" meltingTemperature="56"><Component hybridizedRange="6515-6541" bases="atagcattatcaacaacctcaaatacc"/></BindingSite></Primer><Primer recentID="1" name="P790 DFTL_1548FR1R" sequence="GCGGCCGCctttttcaaaatttcaaaatgaacctatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6825-6861" boundStrand="1" annealedBases="GCGGCCGCctttttcaaaatttcaaaatgaacctatc" meltingTemperature="65"><Component hybridizedRange="6825-6861" bases="GCGGCCGCctttttcaaaatttcaaaatgaacctatc"/></BindingSite><BindingSite simplified="1" location="6825-6861" boundStrand="1" annealedBases="GCGGCCGCctttttcaaaatttcaaaatgaacctatc" meltingTemperature="65"><Component hybridizedRange="6825-6861" bases="GCGGCCGCctttttcaaaatttcaaaatgaacctatc"/></BindingSite></Primer><Primer recentID="2" name="P789 DFTL_1548FR1F" sequence="GGATCCtcataatcaaagtcaacaaatgaaaacg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6101-6134" boundStrand="0" annealedBases="GGATCCtcataatcaaagtcaacaaatgaaaacg" meltingTemperature="60"><Component hybridizedRange="6101-6134" bases="GGATCCtcataatcaaagtcaacaaatgaaaacg"/></BindingSite><BindingSite location="2698-2711" boundStrand="1" annealedBases="aacaaataaaacg" meltingTemperature="30"><Component bases="GGATCCtcataatcaaagtc"/><Component hybridizedRange="2705-2711" bases="aacaaat"/><Component bases="g"/><Component hybridizedRange="2698-2703" bases="aaaacg"/></BindingSite><BindingSite simplified="1" location="6101-6134" boundStrand="0" annealedBases="GGATCCtcataatcaaagtcaacaaatgaaaacg" meltingTemperature="60"><Component hybridizedRange="6101-6134" bases="GGATCCtcataatcaaagtcaacaaatgaaaacg"/></BindingSite><BindingSite simplified="1" location="2698-2711" boundStrand="1" annealedBases="aacaaataaaacg" meltingTemperature="30"><Component bases="GGATCCtcataatcaaagtc"/><Component hybridizedRange="2705-2711" bases="aacaaat"/><Component bases="g"/><Component hybridizedRange="2698-2703" bases="aaaacg"/></BindingSite></Primer><Primer recentID="3" name="P791 DFTL_1548FR2F" sequence="GCGGCCGCTcctttataattttataacaacttaaatctc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6854-6892" boundStrand="0" annealedBases="GCGGCCGCTcctttataattttataacaacttaaatctc" meltingTemperature="62"><Component hybridizedRange="6854-6892" bases="GCGGCCGCTcctttataattttataacaacttaaatctc"/></BindingSite><BindingSite simplified="1" location="6854-6892" boundStrand="0" annealedBases="GCGGCCGCTcctttataattttataacaacttaaatctc" meltingTemperature="62"><Component hybridizedRange="6854-6892" bases="GCGGCCGCTcctttataattttataacaacttaaatctc"/></BindingSite></Primer><Primer recentID="4" name="P792 DFTL_1548FR2R" sequence="GGTACCtgatactgtagacaacatcatatttaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7460-7493" boundStrand="1" annealedBases="GGTACCtgatactgtagacaacatcatatttaag" meltingTemperature="58"><Component hybridizedRange="7460-7493" bases="GGTACCtgatactgtagacaacatcatatttaag"/></BindingSite><BindingSite simplified="1" location="7460-7493" boundStrand="1" annealedBases="GGTACCtgatactgtagacaacatcatatttaag" meltingTemperature="58"><Component hybridizedRange="7460-7493" bases="GGTACCtgatactgtagacaacatcatatttaag"/></BindingSite></Primer></Primers>
  ?<Notes>
<UUID>a88c6f5d-5084-478a-beae-4978f002ae43</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:54:14">2018.6.6</Created>
<LastModified UTC="13:9:3">2018.6.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         Unsaved Enzyme Set                                                                                           d     CHO  d              E              Y? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   <?xml version="1.0"?><CustomEnzymeSets><CustomEnzymeSet type="2" name="Unsaved Enzyme Set" enzymeNames="NotI"/></CustomEnzymeSets>
