	   SnapGene      tatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacaatcgatcaagggttggtttgcgcattcacagttctccgcaagaattgattggctccaattcttggagtggtgaatccgttagcgaggtgccgccggcttccattcaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgatctatacccttcaagctttgaaaaataaacttaattattatatatgttatttagctagtttttttaattaaagttaaaatcgagagcttgtttgacaaaaaaacaaaaaaatttcttgaaaattttttttttgactcaatatctagacttgcaagagcttggaactttgagattgttctaagatgcatacaaattcaaaatgcttaaacaaaaataatttaacaaaggagtaagattgttatgagccatattcaacgggaaacgtcttgctcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggaaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataaacttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagaattggttaattggttgtaacactggcagagcattacgctgacttgacgggacggcggctttgttgaataaatcgaacttttgctgagttgaaggatcagatcacgcatcttcccgacaacgcagaccgttccgtggcaaagcaaaagttcaaaatcaccaactggtccacctacaacaaagctctcatcaacgatccagctgtcccaaacgtccaggccttttgcagaagagatatttttaattgtggacgaatcgaactcaggaacttgatatttttcatttttttgctgttcagggatttgcagcatatcatggcgtgtaatatgggaaatgccgtatgtttccttatatggcttttggttcgtttctttcgcaaacgcttgagttgcgcctcctgccagcagtgcggtagtaaaggttaatactgttgcttgttttgcaaactttttgatgttcatcgttcatgtctccttttttatgtactgtgttagcggtctgcttcttccagccctcctgtttgaagatggcaagttagttacgcacaataaaaaaagacctaaaatatgtaaggggtgacgccaaagtatacactttgccctttacacattttaggtcttgcctgctttatcagtaacaaacccgcgcgatttacttttcgacctcattctattagactctcgtttggattgcaactggtctattttcctcttttgtttgatagaaaatcataaaaggatttgcagactacgggcctaaagaactaaaaaatctatctgtttcttttcattctctgtattttttatagtttctgttgcatgggcataaagttgcctttttaatcacaattcagaaaatatcataatatctcatttcactaaataatagtgaacggcaggtatatgtgatgggttaaaaaggatcgatcctctagctagagtcgatcttcgccagcagggcgaggatcgtggcatcaccgaaccgcgccgtgcgcgggtcgtcggtgagccagagtttcagcaggccgcccaggcggcccaggtcgccattgatgcgggccagctcgcggacgtgctcatagtccacgacgcccgtgattttgtagccctggccgacggccagcaggtaggccgacaggctcatgccggctgccgccgccttttcctcaatcgctcttcgttcgtctggaaggcagtacaccttgataggtgggctgcccttcctggttggcttggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccagcctcgcagagcaggattcccgttgagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaaggatggatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaagcgctgcttccctgctgttttgtggaatatctaccgactggaaacaggcaaatgcaggaaattactgaactgaggggacaggcgagagacgatgccaaagagctacaccgacgagctggccgagtgggttgaatcccgcgcggccaagaagcgccggcgtgatgaggctgcggttgcgttcctggcggtgagggcggatgtcgatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgcatatttgaatgtatttagaaaaataaacaaaagagtttgtagaaacgcaaaaaggccatccgtcaggatggccttctgcttaatttgatgcctggcagtttatggcgggcgtcctgcccgccaccctccgggccgttgcttcgcaacgttcaaatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgccgccaggcaaattctgttttatcagaccgcttctgcgttctgatttaatctgtatcaggctgaaaatcttctctcatccgccaaaacagccaagctcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgccaagcttgcatgcctgcagatatacccttcaagctttgaaaaataaacttaattattatatatgttatttagctagtttttttaattaaagttaaaatcgagagcttgtttgacaaaaaaacaaaaaaatttcttgaaaattttttttttgactcaatatctagacttgcaagagcttggaactttgagattgttctaagatgcatacaaattcaaaatgcttaaacaaaaataatttaacaaaggagtaagattgttatgaacattcggatccccgggtaccaagaaaatagctcctaatggtgttttccctattttgatggagaaagactatagcatcaataatagaaaagcattgctaatttatattgatgaaagatttcctgctccaagcttgctaccaaatgtagttaatgagcgcataaagatacgcttatcgcttgataaaattgataatgagtggtatccagtattagatcaaattcgcaaacacagatcagatcagaagatgcttgaatcaatgtttaaagacctaaaagaaagtttattggcaatggaaaaagcttttactggctcagagttttttatatcttctggctttactttagcagattgttatatagctgctttgattatatgtttagaagc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                        	                        (                                            "      "   !      %                                                             $         /   c   3         !   #   !                              !   $            (                                              	                                         '            &   
                              +   !                                 0      "         "      .   #   R   #                                                                                                          #      $               ,                     	                        	                  $         !                  "                                              7ZD	x \[E_fyv 8	dd nq

><5kx	 \Ab/)  H/F`2f$pgs/w @ ]x0
b
D:>9tz\	 z Z p  9	l90Gs   9 O    h@	}J_  j).%	JSe! g 9d3'q		;
e5! W or?z-6tlC\" ,  _
	

K(~ ~ 'A+P/=N W#;= s <JqT[r
Ty M 7]oB3 j5ic0K* 	 c  	B7s	+u) I QGo2eMnF k    07Gf	%^	hkqt0x*  Mragk*U

	4GqLeP$o,C]J :3"T~(r  I \	z

T# &`c2AipF		;:Yw&Z FOkQ
5?@H
{]  A ^l	Hw;

1
c
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k
a~0 D	
X'L n q  F:xI:} 9	

m1JH	 i	HF~ [ 	^0t; T   	~~  : DK 			^ .` P   iA	
)K Xd	,  /Y	/	

K"c  WLk&	k			rf'BNPh(   *`u6		,
M


wTn3S+f?% e I	P	'69G 4Z-fi=6 e6  :f	bV	r		<
 Z  (MV%d  X  Xp	o	b{n.7EvAH] K N3ux5
@2mD X I%\4	#{  
s	
Y`vu[/=  - 0 F  `]c	h
 ;})akGn   S;		5
] Mfp

Lnw7Hw I	T"   ,QJ>x!XV<,> ]  'P/Mt" (m(02	v		Od{Op<YU!   ABkl8Di$W 1Q3? !$),r[ wpR% & 3  KS|		7q]6?HO t uS;U\
		s

gzzh =		AhU [m	=c4jw ? w@a
C9a.s N 28g$		8 V ^IpZ'sSV[! H b  h*t1L(    69j)eT
y

d+Bjp'q "kGj	XG`I  b!	$
j
/ m E
8	
}  C			+ d   _t			

v2A* 
 a,Y J  2 Hn	mu3Gz / E   L	:|`6GFmv  l7I&C.NoU;P2 2  RR	p\f>o ZvfH?#		`
&8`RirZ$    !8Fig		x
._ & l `		
@|   1kK6_t
BFnlu1y/  u>h5"			_
w


7sQ?-E^{Yc   Nstst#0189					V
^
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"KMg&Ffqz"K   Oy,b&t+R=	 
@L:^ A%	MmU{! v3#;U)			



Nh<?#b# 6|4Yt
;grP)L]! {;I}X


#L'G0s JU\c&,AS
	M~ <   -;]2	AD;_g x Pz'u
#>	7
{
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U BhN>	
<' hO
a BRqV[ R G	T	b		;%|d4 G'&9d3njiCZx kR


6	4@S9! ?w4$rGV	\



SI=$y|$ V<Pl	

+@Ay!#U2Qb b*		BOijc^ 
ICZu2
JW
K}M	 B mx$!
<_z(  _75O<en	
@
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[)
 OV e		Bi +6Np }Z)*  +  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                            %                                                         	                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
                                                                                                                                (                                                                                        
                                                                                                                                   #                                    
                           	                         
                                    "                	               e   Z         P   S   #            G                                                                                                                                                                                 %                           	                                                                                                                                                                                                                                                                                                                                       
                                                    (                                    
                                               #            
         	         
            "      	     e Z   P S #    G   h  i  i  j  lu  po  vu \[\S"
a1 $]U7OF			2	7Y%.n6?OmHtQ
~Q
~Q
~ IX v9kv y0Z	Z  rUF	{3fq( r	ul8r\V^ 2ruAr  	n}+YI$wn4f	=|f $Q		7}dwDvpD"x	t63e .  	9{ (dnkn(x	
,6 g  f"\cK!,Ikt AI$w Hl*lH l 	[.3c	+ixD #zDi hp YZ5	}mXG*{,	V	- 0  >kxXV,><'q
Tx >~	l0~pO8'- 0  k>xXV,>LF^	;&| -
[- 0  !p)r >kxXV,>AR >~XG*{,Ln7&c2iYw) 6 ($8
  W:9 M _1B+%cM ' ,	f	`eB+c%D	( 5 t#>P	X
)Xbv	 :~%
+~rOYh ;A
&I fCqG%hqBi3:  k			r  7f	{>y	2)}5	 +%
~	:~r!p)r AR( 5 Dt	# R[!bJ	Q	
V0U	MWWt

Xa  "L*s
WbRl4U*SfX  Xp	o	b{n.7EvAH]Xd	, l7I&C.NoU;P2 
s	
Y`vu[/= $(0 #+y]zD0Eos <JqT[r
Ty  %?-xqZgFgS	
32 s ~   '<AJRlRq+PT/7I[	r
&p=N\Tf >CWy.No#;U;o=P2Q gFj>aNU8gq=QS	a			[




*ARhBx "$3T1PRac38ixUdT
 Heo\b:	4
g

j   %?-P]VLGX		}			8rGx&*W{fCq,DOZa * 7 >   %1?-)Rd%Y9
<~"Hxb+r-|U!9qv -Z6|4Yt
;grP)L]T<		
E
!j{7M 7]oB3X}*9yr'	
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&08
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.>_I   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  &;<?xml version="1.0"?><Features nextValidID="25"><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-5877" color="#ffffff" type="standard"/><Q name="mol_type"><V predef="other DNA"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="1" name="/dnas_title=pKL13 Verified" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4353-4601" color="#ffffff" type="standard"/><Q name="db_xref"><V text="119857" predef="taxon"/></Q><Q name="mol_type"><V predef="genomic DNA"/></Q><Q name="note"><V text="/dnas_title=pKL13 Verified"/></Q><Q name="organism"><V text="Francisella tularensis subsp. holarctica"/></Q><Q name="strain"><V text="LVS"/></Q><Q name="sub_species"><V text="holarctica"/></Q></Feature><Feature recentID="2" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" 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type="standard"/><Q name="label"><V text="P049 groES promoter F"/></Q><Q name="note"><V text="P049 groES promoter F"/></Q></Feature><Feature recentID="6" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="7" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="8" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="label"><V text="P027 pEX18KanSeq3F"/></Q><Q name="note"><V text="P027 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promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4353-4601" color="#ffffff" type="standard"/><Q name="note"><V text="groES promoter"/></Q></Feature><Feature recentID="16" name="P052 groESpromoF" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4353-4381" color="#a6acb3" type="standard"/><Q name="label"><V text="P052 groESpromoF"/></Q><Q name="note"><V text="P052 groESpromoF"/></Q></Feature><Feature recentID="17" name="P051 groESpromoR" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4574-4601" color="#a6acb3" type="standard"/><Q name="label"><V text="P051 groESpromoR"/></Q><Q name="note"><V text="P051 groESpromoR"/></Q></Feature><Feature recentID="18" name="groES" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4592-4601" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3951567" predef="GeneID"/></Q><Q name="gene"><V text="groES"/></Q><Q name="label"><V text="groES"/></Q><Q name="locus_tag"><V text="FTL_1715"/></Q></Feature><Feature recentID="19" name="groES" directionality="1" translateFirstCodonAsMet="1" translationMW="357.33" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4621" maxFusedRunOn="4621"><Segment range="4592-4601" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256984" predef="GI"/><V text="3951567" predef="GeneID"/></Q><Q name="gene"><V text="groES"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="label"><V text="groES"/></Q><Q name="locus_tag"><V text="FTL_1715"/></Q><Q name="note"><V text="10 kDa chaperonin; Cpn10; GroES; forms&#10;homoheptameric ring; binds to one or both ends of the GroEL double barrel in the presence of adenine nucleotides capping it; folding of unfolded substrates initiates in a GroEL-substrate bound and capped by GroES; release of the folded substrate is dependent on ATP binding and hydrolysis in the trans ring"/><V text="/dnas_title=co-chaperonin GroES"/></Q><Q name="product"><V text="co-chaperonin GroES"/></Q><Q name="protein_id"><V text="YP_514346.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="HSD"/></Q></Feature><Feature recentID="20" name="FTL_1606" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="5160" maxFusedRunOn="5160"><Segment range="4612-5120" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256883" predef="GI"/><V text="3951956" predef="GeneID"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="label"><V text="FTL_1606"/></Q><Q name="locus_tag"><V text="FTL_1606"/></Q><Q name="note"><V text="/dnas_title=stringent starvation protein A fragment, regulator of transcription"/></Q><Q name="product"><V text="stringent starvation protein A, regulator of transcription"/></Q><Q name="protein_id"><V text="YP_514245.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="KKIAPNGVFPILMEKDYSINNRKALLIYIDERFPAPSLLPNVVNERIKIRLSLDKIDNEWYPVLDQIRKHRSDQKMLESMFKDLKESLLAMEKAFTGSEFFISSGFTLADCYIAALIICLEAEGFIIDDEYGAIYEYKKRLFARDSVKKANIKGGAGESLLKTLRTHRA"/></Q></Feature><Feature recentID="21" name="VSVG peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5125-5160" color="#cc99b2" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="VSVG"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="22" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment 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  9<?xml version="1.0"?><Primers nextValidID="1"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="P035 pEX18integrationVSVG" sequence="tttacctaatctattcatttcaatatcag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5128-5156" boundStrand="1" annealedBases="tttacctaatctattcatttcaatatcag" meltingTemperature="52"><Component hybridizedRange="5128-5156" bases="tttacctaatctattcatttcaatatcag"/></BindingSite><BindingSite simplified="1" location="5128-5156" boundStrand="1" annealedBases="tttacctaatctattcatttcaatatcag" meltingTemperature="52"><Component hybridizedRange="5128-5156" bases="tttacctaatctattcatttcaatatcag"/></BindingSite></Primer></Primers>
  q<Notes>
<UUID>947206a8-50b3-4290-b0d9-81a52f903fc9</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Description>synthetic circular DNA</Description>
<Created UTC="4:0:0">2018.7.21</Created>
<LastModified UTC="15:52:58">2019.9.5</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         Unique 6+ Cutters                                                                                            d     CHO  d              E              Y? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
