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dRt[]0%:v 2  RRj
M>*NV4Yn  ZvfH?
Sy..z 7dtD&    !8FiN	gq5[zFem q l `n	DNc}f    1kK6i	


-B_mc1  u>h5m	Rpy-EAdycelCpT   Nstst#0189l				uvwx$,MNO@4YZcwxkwu9`A u$2{l:XH	P		"%Pu~5[lx Faq~4iw5,   Oy,b&t+L			
 m_qA&v-:h A%	Mm;	##vN5|2 v3#;U	I	Q		r]6\qy	S2r
-jL) 6|4Y
_B,!Rx6G3 {;I}x
9YiQM&QmGb] JU\c&,A
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tn`	eOtQt! x Pz'u
#N

iRZI-B   e			-#|'w.i@ BhN>D^
_ hO
a BRq(			f$#}% R <	

$"0	Ok(6w& G'&9d3njo	
R

5k7w(F kR		M tOs.#t) ?w4$rGVcJ		v
	s*!]z
cf , V<PE	4	:`^G]_T&3k  b0	mz7Tr9TMH 
ICZ	 	c


(C g157% B mx$				SzD>  _75O<e	6	@	c	

ntU`<bi|2mISA-"BSy .X}6df <GH!  # y
[]b[Nt>1 p<H~>			Y
 d:FZjR''>n.H7^#  ,z^$&8RV<`6KKm%  $|!NT{JGzj. aVc=U | 5]0'-		=}<O  KEB	#JV Udz	
-|"} ?QVtlCsV	i{ = `  
'u	
>o$
fd_Y"  dy.<^03N	

wpauEO 2	a			&Yr Ru   	ul pPM ) 6 (u$h

+j4h0. y  #O
:JntrD!  {vI[CV;U QZnSW?  K ]o$sd9	C  Er f	s
3q}/AR   ,u			.}"xJ  d'g(j7 C	`3r GeGZj ! 8 o     ci-?&EA 3`# + r ;*OS	8
H
'_K^(h  iPr)	j
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x^iD$ =.Cx				
1% G-T%;E6Xl|/1x$ S =	
e
VUo1jPlLci>  KX5






*'/=s8UY|?S0)IZ (90 "	 
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Ici#
M7M"  w4S


-l|)(C*&!  Heo\	p
6X058xq> \  'jk
~lpCHPni *  (:{pq	

[5Gw; U lS		
`I  C	2N!VnP 0WguFt D   c;M\/e$u  YE	
M-|lD x+z%sWK		Q
!ot+DL^v%Et,_gp}3dk, @~5LH|w

XA"+	{K ; w :%d	M

s{Ng Q N8=QnQ
C/6' ja	s S^8N,  WB	
		5	?		
;alHR@RA4CF~(   "  oSF			%U_J`Ul<6T { 4,{k<B|UUN _  x&/t1	=Xqc Ymg
*3VUs:  c\nc8pBI   b,7
@225  i  )p0?ZDg	8	


7w]XhmKC65  J!		d





)H:bDWk{LY8-  iz[

}ZW/o~4~4=h C B	_1bH:fUE5 X}*9y	L


r
n@{*$D+^1 ! nM7R						
$.r5?IR B};S  l%W)jATAK    (ao+>yf7	




|v?Va.n}W3VJjB<K: 86Q	A	
q	a#Gm?-qT>)HQ#CA]|     /`P=x23vw 	




opKLMuF
-=>cdjklm*+,_nopU=%&'(./019:;EFGNOPT\WXYz{9 04ax	d			

,1 BNe[`xF#+82=UyZp*Un  		2,9M[$]3 }/h Y!5b	

!CO_fuaG$!3=q' f 1y

)7F\ey>Vz%Vo& *~@Y	e	-IgKQXH+I2  	A
z
8u?%^ 5[mnc\O-O9\  	\^5:!# $ V	3KebwZ[N/ C0	#
-
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;P=UvH(?"8_$ qi!ZI6?	
ego	>rA. f="c<&		"D[k`Sg (b%:4/I!r  S~p		!GC=y   ;c	


%AWhz=& 2_z}	*Sa6?LZ{-  g  -{g>


'8DkG] )K7LWwn 
:$		9;%fY+Wp  AUZ	/>3O  +D}O){k%JbJV) %\K
.'RWdI,- "|Hf		hLRYY 6Y^
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>1nE_  .1-		

z=\E  Y  (			?>9J@3HP# }  &@q.V_U
{
wYGQv~=&, L 64n6F	qW/ Lj	B]KP!~+aP+ %\vyoCd<x:  OC$f
.]5 +~$f WJ	 .A+n+ V	

]_z}~k;"  Ye{		!
+
#6 	MWWt\			)
u&/x RK^	
m[!;Dt\*
j%   @4f1EyO@	(>k|$ % #t+wL(QcDuWQ) > 1}	$

'Vw
LU^bx0pz@#`l
! G a a (
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27A]%+Z    D^Y~Q &Ne`* . 1  Qv		.

?<>{LIs)U:Zg9  ejJ_Mh

fxbs0   r"/=74O	

tbv@jhvB~!  z	8hqp1F  T["1@
,d
?sP gFg'			!^v  >Uqb3	u
l)F\"Sbs' -N~#d	b			~
'ms5Thcf0$  MG =yX
Z#E\aZ&-;=s5J  T	"z `			vo#kDLNX ( 5  tHmqCT>  Jq+t	r
r!Q * 7  )Rd%G
iJ]_'q   <-^Mw		i
O*01& W


&,Tnhr5TXi{>R/K'./L~    v
kBGO'p):	  
[ v ${~	CKuksf|2z `m	P
TQ7@ME ^ z  3w.s+;bUo{%  h  h	

69\ 3zX   |)K
 @C '.nw1	


0J"El,)^*7$8DM<@xVoTm!  |)g? 
(^EHJLW\ Ulm	vYZ"# BRo;%	f<T9B<@qx   =9		:gXJY* C[	&Q,X   %?-U^	
FP[D $Bi	p *e	
4 V 
y*jm(  "L*sJ[	%P0t :? =P#  P|} z&K@T]rTw%Y*	  !gc0 ,E~LTF}	t
l4e,~r[~ ' 4  s*s	jBSKW$ ]VLv	N/SNIJ-p.9K u v-lr	~BTey"  &f	

/!]+(6#yS[ T<	P8XIX!  >#~ITh		
i)l S\$iZ) e	O7MS 8Z }g	(hk  /  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                    ,                                                           
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
                                                                                                                                                                                                                                                                     *                                       	                                                                                                                       	                        	      	         
               &                  	   	                                          	                     
                                       (   (      )   !   	      $            t      !   `   `   )         5   `                                                                                                                                                                         ,                           
                                                                                                                                                                                                                                                                    
                                                                                                         *               	                                           	        	  	   
     &      	 	              	       
             ( (  ) ! 	  $    t  ! ` ` )   5 `           U  S  
u I	*r)*X="/1 #q
^OR4cccc	XvS 'OR[dS<;o2*B
-
-
--- 5I&Zc eDkDBN	 y	(y	( ru?*	0
wQ{3^ Qw ry	Cu|o
oo
lo
b	"r.	F$^ sTM~ruCwiArW	u 6<d,}|S\pC$]EO<		o
oof&		u=J4 $QF
yKmqu2EOSDX\.
	u,"xh6t	m#C]OD .  
I kOdn
k(o

F} g  f"\cK&			!eU^ Ab3$EO .
<:ZlZ l6	2[e
o1d{ixb6 #zii hp YZ5		8	
XK6=q|&T]wIp		#		$	d- 0  	9F&o(<'R	?Zo"F >~T	
		:~p'- 0  	9F&o(LF,~r]	| -}< x )- 0  !p)r	T
vOX9F&o(+< >~T|	
&T]wIp	xW
Y<&c2i

4j'E) 6 ($h } %

i! M y-B<Ti
O1 ' 		
+4.d_OB~	i
<OT1kDQ `( 5 t>PsX*
IX0
:
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3 f][G6B	
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type="rRNA" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7400-8106" color="#00ff00" type="standard"/><Q name="product"><V text="16S ribosomal RNA"/></Q><Q name="inference"><V bool="1" text="AJ749949.1" predef="similar to DNA sequence"/></Q><Q name="note"><V text="/dnas_title=16S ribosomal RNA"/></Q><Q name="db_xref"><V text="3952047" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_R0003"/></Q></Feature><Feature recentID="31" name="FTL_R0003" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7400-8106" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952047" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_R0003"/></Q></Feature><Feature recentID="30" name="FTL_0109" directionality="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7103" maxFusedRunOn="7103"><Segment range="7077-7085" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="YP_512907.1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="db_xref"><V text="89255546" predef="GI"/><V text="3952285" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0109"/></Q></Feature><Feature recentID="29" name="FTL_0109" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7077-7085" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952285" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0109"/></Q></Feature><Feature recentID="28" name="FTL_0108" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" 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consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8530-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="7" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8176-8182" color="#ffffff" type="standard"/><Segment range="8183-8200" color="#ffffff" type="standard"/><Segment name="-35" range="8201-8206" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8221-8242" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8128-8144" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8152-8168" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  @<Notes>
<UUID>3402cadd-1352-42d4-ac5a-2d9d0567bb9f</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:6:22">2018.7.6</Created>
<LastModified UTC="12:19:56">2018.7.9</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  c      |  G        Gf0m祤]-vSznhyӖTUHfITeU*5UUVUjA2ib
A9BAA&            :  C  F  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="6"><Feature recentID="5" name="FTL_0126" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="12-21" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="hypothetical protein" directionality="2" translationMW="333.46" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="1" maxFusedRunOn="1"><Segment range="12-21" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="45-45" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="2" name="-10" directionality="2" type="-10_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="52-57" color="#00ff00" type="standard"/><Q name="note"><V text="-10"/></Q></Feature><Feature recentID="1" name="-35" directionality="2" type="-35_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="75-80" color="#00ff00" type="standard"/><Q name="note"><V text="-35"/></Q></Feature><Feature recentID="0" name="PRE" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="87-93" color="#0080ff" type="standard"/><Q name="note"><V text="PRE"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P822 DFPI_FR1F2" sequence="atatCCTGCAGGatatcctagaaaccttagctcctaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="289-326" boundStrand="1" annealedBases="atatCCTGCAGGatatcctagaaaccttagctcctaac" meltingTemperature="63"><Component hybridizedRange="289-326" bases="atatCCTGCAGGatatcctagaaaccttagctcctaac"/></BindingSite><BindingSite simplified="1" location="289-326" boundStrand="1" annealedBases="atatCCTGCAGGatatcctagaaaccttagctcctaac" meltingTemperature="63"><Component hybridizedRange="289-326" bases="atatCCTGCAGGatatcctagaaaccttagctcctaac"/></BindingSite></Primer><Primer recentID="1" name="P823 DFPI_FR1R2" sequence="atatGCGGCCGCtgctatcattgtctacttaattagaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-39" boundStrand="0" annealedBases="atatGCGGCCGCtgctatcattgtctacttaattagaaca" meltingTemperature="66"><Component hybridizedRange="0-39" bases="atatGCGGCCGCtgctatcattgtctacttaattagaaca"/></BindingSite><BindingSite simplified="1" location="0-39" boundStrand="0" annealedBases="atatGCGGCCGCtgctatcattgtctacttaattagaaca" meltingTemperature="66"><Component hybridizedRange="0-39" bases="atatGCGGCCGCtgctatcattgtctacttaattagaaca"/></BindingSite></Primer></Primers>
  f<Notes>
<UUID>4d155228-4a0c-43fc-8b8b-06c621d6faf8</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:19:9">2018.7.9</Created>
<LastModified UTC="12:20:16">2018.7.9</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  f	MTNVUfWT#j&}#jHXUZxUH"
jNk=mu:]Wb'T)YNnRjae*]FTy*AUUuwTYvme:eT{fYjSTc&-ZU&JJ:JvFJB"5}9fdTXnjN&Whh2&          
  
      <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="14"><Feature recentID="13" name="FTL_0108" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="11-41" color="#00e600" type="standard"/></Feature><Feature recentID="12" name="hypothetical protein" directionality="2" translationMW="1212.65" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="1" maxFusedRunOn="3"><Segment range="11-41" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="FTL_0109" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="134-535" color="#00e600" type="standard"/></Feature><Feature recentID="10" name="hypothetical protein" directionality="1" translationMW="15254.63" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="134-535" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="FTL_0110" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="545-748" color="#00e600" type="standard"/></Feature><Feature recentID="8" name="hypothetical protein" directionality="1" translationMW="7682.98" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="545-748" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="P094 RT-PCR primers for ChIP iglA #2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="677-698" color="#8080ff" type="standard"/><Q name="note"><V text="P094 RT-PCR primers for ChIP iglA #2"/></Q></Feature><Feature recentID="6" name="P093 RT-PCR primers for ChIP iglA #2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="565-587" color="#8080ff" type="standard"/><Q name="note"><V text="P093 RT-PCR primers for ChIP iglA #2"/></Q></Feature><Feature recentID="5" name="-35 element" directionality="1" type="-35_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="766-771" color="#00ff00" type="standard"/><Q name="note"><V text="-35 element"/></Q></Feature><Feature recentID="4" name="PRE" directionality="1" type="misc_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="753-759" color="#0080ff" type="standard"/><Q name="note"><V text="PRE"/></Q></Feature><Feature recentID="3" name="P318 iglA_PREckF" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="539-568" color="#8080ff" type="standard"/><Q name="note"><V text="P318 iglA_PREckF"/></Q></Feature><Feature recentID="2" name="P321 PiglA_M1_1R *MUTATES PRE!*" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="736-763" color="#8080ff" type="standard"/><Q name="note"><V text="P321 PiglA_M1_1R *MUTATES PRE!*"/></Q></Feature><Feature recentID="1" name="P322 PiglA_M1_2F *MUTATES PRE!*" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="750-777" color="#8080ff" type="standard"/><Q name="note"><V text="P322 PiglA_M1_2F *MUTATES PRE!*"/></Q></Feature><Feature recentID="0" name="P324 PiglA_M1_Seq1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="237-265" color="#8080ff" type="standard"/><Q name="note"><V text="P324 PiglA_M1_Seq1"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P825 DFPI_FR2R2" sequence="atatGGTACCaaagcaaaagctttagctttaataattgc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-38" boundStrand="0" annealedBases="atatGGTACCaaagcaaaagctttagctttaataattgc" meltingTemperature="61"><Component hybridizedRange="0-38" bases="atatGGTACCaaagcaaaagctttagctttaataattgc"/></BindingSite><BindingSite simplified="1" location="0-38" boundStrand="0" annealedBases="atatGGTACCaaagcaaaagctttagctttaataattgc" meltingTemperature="61"><Component hybridizedRange="0-38" bases="atatGGTACCaaagcaaaagctttagctttaataattgc"/></BindingSite></Primer><Primer recentID="1" name="P824 DFPI_FR2F2" sequence="atatGCGGCCGCTTAAcgccaataacacaatgtttatacagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="753-794" boundStrand="1" annealedBases="atatGCGGCCGCTTAAcgccaataacacaatgtttatacagc" meltingTemperature="68"><Component hybridizedRange="753-794" bases="atatGCGGCCGCTTAAcgccaataacacaatgtttatacagc"/></BindingSite><BindingSite simplified="1" location="753-794" boundStrand="1" annealedBases="atatGCGGCCGCTTAAcgccaataacacaatgtttatacagc" meltingTemperature="68"><Component hybridizedRange="753-794" bases="atatGCGGCCGCTTAAcgccaataacacaatgtttatacagc"/></BindingSite></Primer></Primers>
  g<Notes>
<UUID>5232a072-63e8-43fe-9f93-3db621bb2a4a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:19:35">2018.7.9</Created>
<LastModified UTC="12:20:35">2018.7.9</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  GU        "~        "~.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAfUtєNeZ-ja_Ua>4kTUVg4TaE	b_asageUg8eUBNR6jHUZDㆶ%eRINeݕR}_J}VfVgU\yڀV>e{iZMꅦ[W׊wEz5SYf$U}YIid~ea5lEy)QgnX	xxe}wU9eUETNYh9eYjViQeuJb:Ƣk6ޤ0ZRU"hf
jhj(&UQTTaUZUT"!ɦb|8p!L)
jQfuwEIm$KEExQ*8 wcR]ă\@}(ǊYeT Oi9( 	@qahw4 Yu=QCSzTP ATYM#O0p LZxB
RшTjID Z"L!XP]|NŬضN*Vo;uw]O6TSa!9{v;x=8X[\£&ξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	          !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ;~<?xml version="1.0"?><Features nextValidID="40"><Feature recentID="39" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8830" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL125 pEXDFPI"/></Q></Feature><Feature recentID="38" name="P773 CkDFPI1R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7128-7156" color="#a020f0" type="standard"/><Q name="note"><V text="P773 CkDFPI1R"/></Q></Feature><Feature recentID="37" name="P772 SeqDFPI1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6686-6715" color="#a020f0" type="standard"/><Q name="note"><V text="P772 SeqDFPI1"/></Q></Feature><Feature recentID="36" name="P768 DFPI_FR1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6107-6141" color="#a020f0" type="standard"/><Q name="note"><V text="P768 DFPI_FR1F"/></Q></Feature><Feature recentID="35" name="P771 DFPI_FR2R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8082-8112" color="#a020f0" type="standard"/><Q name="note"><V text="P771 DFPI_FR2R"/></Q></Feature><Feature recentID="34" name="P770 DFPI_FR2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7086-7126" color="#a020f0" type="standard"/><Q name="note"><V text="P770 DFPI_FR2F"/></Q></Feature><Feature recentID="33" name="P769 DFPI_FR1R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7058-7093" color="#a020f0" type="standard"/><Q name="note"><V text="P769 DFPI_FR1R"/></Q></Feature><Feature recentID="32" name="FTL_R0003" directionality="1" type="rRNA" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7400-8106" color="#00ff00" type="standard"/><Q name="product"><V text="16S ribosomal RNA"/></Q><Q name="inference"><V bool="1" text="AJ749949.1" predef="similar to DNA sequence"/></Q><Q name="note"><V text="/dnas_title=16S ribosomal RNA"/></Q><Q name="db_xref"><V text="3952047" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_R0003"/></Q></Feature><Feature recentID="31" name="FTL_R0003" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7400-8106" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952047" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_R0003"/></Q></Feature><Feature recentID="30" name="FTL_0109" directionality="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7103" maxFusedRunOn="7103"><Segment range="7077-7085" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="YP_512907.1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="db_xref"><V text="89255546" predef="GI"/><V text="3952285" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0109"/></Q></Feature><Feature recentID="29" name="FTL_0109" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7077-7085" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952285" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0109"/></Q></Feature><Feature recentID="28" name="FTL_0108" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6113-6984" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="YP_512906.1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="db_xref"><V text="89255545" predef="GI"/><V text="3952284" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0108"/></Q></Feature><Feature recentID="27" name="FTL_0108" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6984" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952284" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0108"/></Q></Feature><Feature recentID="26" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="25" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="24" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="23" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="22" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="21" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="20" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="19" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8161-8180" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="18" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8139-8164" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="17" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="16" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="15" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="14" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="13" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="12" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8530-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="7" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8176-8182" color="#ffffff" type="standard"/><Segment range="8183-8200" color="#ffffff" type="standard"/><Segment name="-35" range="8201-8206" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8221-8242" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8128-8144" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8152-8168" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  @<Notes>
<UUID>3402cadd-1352-42d4-ac5a-2d9d0567bb9f</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:6:22">2018.7.6</Created>
<LastModified UTC="12:19:56">2018.7.9</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  c      |  G        Gf0m祤]-vSznhyӖTUHfITeU*5UUVUjA2ib
A9BAA&            :  C  F  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="6"><Feature recentID="5" name="FTL_0126" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="12-21" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="hypothetical protein" directionality="2" translationMW="333.46" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="1" maxFusedRunOn="1"><Segment range="12-21" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="45-45" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="2" name="-10" directionality="2" type="-10_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="52-57" color="#00ff00" type="standard"/><Q name="note"><V text="-10"/></Q></Feature><Feature recentID="1" name="-35" directionality="2" type="-35_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="75-80" color="#00ff00" type="standard"/><Q name="note"><V text="-35"/></Q></Feature><Feature recentID="0" name="PRE" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="87-93" color="#0080ff" type="standard"/><Q name="note"><V text="PRE"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P822 DFPI_FR1F2" sequence="atatCCTGCAGGatatcctagaaaccttagctcctaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="289-326" boundStrand="1" annealedBases="atatCCTGCAGGatatcctagaaaccttagctcctaac" meltingTemperature="63"><Component hybridizedRange="289-326" bases="atatCCTGCAGGatatcctagaaaccttagctcctaac"/></BindingSite><BindingSite simplified="1" location="289-326" boundStrand="1" annealedBases="atatCCTGCAGGatatcctagaaaccttagctcctaac" meltingTemperature="63"><Component hybridizedRange="289-326" bases="atatCCTGCAGGatatcctagaaaccttagctcctaac"/></BindingSite></Primer><Primer recentID="1" name="P823 DFPI_FR1R2" sequence="atatGCGGCCGCtgctatcattgtctacttaattagaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-39" boundStrand="0" annealedBases="atatGCGGCCGCtgctatcattgtctacttaattagaaca" meltingTemperature="66"><Component hybridizedRange="0-39" bases="atatGCGGCCGCtgctatcattgtctacttaattagaaca"/></BindingSite><BindingSite simplified="1" location="0-39" boundStrand="0" annealedBases="atatGCGGCCGCtgctatcattgtctacttaattagaaca" meltingTemperature="66"><Component hybridizedRange="0-39" bases="atatGCGGCCGCtgctatcattgtctacttaattagaaca"/></BindingSite></Primer></Primers>
  f<Notes>
<UUID>4d155228-4a0c-43fc-8b8b-06c621d6faf8</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:19:9">2018.7.9</Created>
<LastModified UTC="12:20:16">2018.7.9</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  f	MTNVUfWT#j&}#jHXUZxUH"
jNk=mu:]Wb'T)YNnRjae*]FTy*AUUuwTYvme:eT{fYjSTc&-ZU&JJ:JvFJB"5}9fdTXnjN&Whh2&          
  
      <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="14"><Feature recentID="13" name="FTL_0108" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="11-41" color="#00e600" type="standard"/></Feature><Feature recentID="12" name="hypothetical protein" directionality="2" translationMW="1212.65" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="1" maxFusedRunOn="3"><Segment range="11-41" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="FTL_0109" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="134-535" color="#00e600" type="standard"/></Feature><Feature recentID="10" name="hypothetical protein" directionality="1" translationMW="15254.63" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="134-535" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="FTL_0110" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="545-748" color="#00e600" type="standard"/></Feature><Feature recentID="8" name="hypothetical protein" directionality="1" translationMW="7682.98" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="545-748" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="P094 RT-PCR primers for ChIP iglA #2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="677-698" color="#8080ff" type="standard"/><Q name="note"><V text="P094 RT-PCR primers for ChIP iglA #2"/></Q></Feature><Feature recentID="6" name="P093 RT-PCR primers for ChIP iglA #2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="565-587" color="#8080ff" type="standard"/><Q name="note"><V text="P093 RT-PCR primers for ChIP iglA #2"/></Q></Feature><Feature recentID="5" name="-35 element" directionality="1" type="-35_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="766-771" color="#00ff00" type="standard"/><Q name="note"><V text="-35 element"/></Q></Feature><Feature recentID="4" name="PRE" directionality="1" type="misc_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="753-759" color="#0080ff" type="standard"/><Q name="note"><V text="PRE"/></Q></Feature><Feature recentID="3" name="P318 iglA_PREckF" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="539-568" color="#8080ff" type="standard"/><Q name="note"><V text="P318 iglA_PREckF"/></Q></Feature><Feature recentID="2" name="P321 PiglA_M1_1R *MUTATES PRE!*" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="736-763" color="#8080ff" type="standard"/><Q name="note"><V text="P321 PiglA_M1_1R *MUTATES PRE!*"/></Q></Feature><Feature recentID="1" name="P322 PiglA_M1_2F *MUTATES PRE!*" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="750-777" color="#8080ff" type="standard"/><Q name="note"><V text="P322 PiglA_M1_2F *MUTATES PRE!*"/></Q></Feature><Feature recentID="0" name="P324 PiglA_M1_Seq1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="237-265" color="#8080ff" type="standard"/><Q name="note"><V text="P324 PiglA_M1_Seq1"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P825 DFPI_FR2R2" sequence="atatGGTACCaaagcaaaagctttagctttaataattgc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-38" boundStrand="0" annealedBases="atatGGTACCaaagcaaaagctttagctttaataattgc" meltingTemperature="61"><Component hybridizedRange="0-38" bases="atatGGTACCaaagcaaaagctttagctttaataattgc"/></BindingSite><BindingSite simplified="1" location="0-38" boundStrand="0" annealedBases="atatGGTACCaaagcaaaagctttagctttaataattgc" meltingTemperature="61"><Component hybridizedRange="0-38" bases="atatGGTACCaaagcaaaagctttagctttaataattgc"/></BindingSite></Primer><Primer recentID="1" name="P824 DFPI_FR2F2" sequence="atatGCGGCCGCTTAAcgccaataacacaatgtttatacagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="753-794" boundStrand="1" annealedBases="atatGCGGCCGCTTAAcgccaataacacaatgtttatacagc" meltingTemperature="68"><Component hybridizedRange="753-794" bases="atatGCGGCCGCTTAAcgccaataacacaatgtttatacagc"/></BindingSite><BindingSite simplified="1" location="753-794" boundStrand="1" annealedBases="atatGCGGCCGCTTAAcgccaataacacaatgtttatacagc" meltingTemperature="68"><Component hybridizedRange="753-794" bases="atatGCGGCCGCTTAAcgccaataacacaatgtttatacagc"/></BindingSite></Primer></Primers>
  g<Notes>
<UUID>5232a072-63e8-43fe-9f93-3db621bb2a4a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:19:35">2018.7.9</Created>
<LastModified UTC="12:20:35">2018.7.9</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature recentID="14" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MNIKKFAKQATVLTFTTALLAGGATQAFA,KETNQKPYKETYGISHITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK*"/></Q></Feature><Feature recentID="15" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" 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name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="20" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="21" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="22" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="23" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="24" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="25" name="PRE" directionality="1" type="misc_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6313-6319" color="#0080ff" type="standard"/><Q name="note"><V text="PRE"/></Q></Feature><Feature recentID="26" name="-35" directionality="1" type="-35_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6326-6331" color="#00ff00" type="standard"/><Q name="note"><V text="-35"/></Q></Feature><Feature recentID="27" name="-10" directionality="1" type="-10_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6349-6354" color="#00ff00" type="standard"/><Q name="note"><V text="-10"/></Q></Feature><Feature recentID="28" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6361-6361" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="29" name="hypothetical protein" directionality="1" translationMW="333.46" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6405" maxFusedRunOn="6405"><Segment range="6385-6394" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MIA"/></Q></Feature><Feature recentID="30" name="FTL_0126" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6385-6394" color="#00e600" type="standard"/></Feature><Feature recentID="31" name="P324 PiglA_M1_Seq1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6920-6948" color="#8080ff" type="standard"/><Q name="note"><V text="P324 PiglA_M1_Seq1"/></Q></Feature><Feature recentID="32" name="P318 iglA_PREckF" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6617-6646" color="#8080ff" type="standard"/><Q name="note"><V text="P318 iglA_PREckF"/></Q></Feature><Feature recentID="33" name="PRE" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment 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type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7278" maxFusedRunOn="7278"><Segment range="7144-7174" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MQLLKLKLLL"/></Q></Feature><Feature recentID="40" name="FTL_0108" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7144-7174" color="#00e600" type="standard"/></Feature></Features>
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meltingTemperature="55"><Component hybridizedRange="7228-7247" bases="tgtggaattgtgagcggata"/></BindingSite><BindingSite simplified="1" location="7228-7247" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="55"><Component hybridizedRange="7228-7247" bases="tgtggaattgtgagcggata"/></BindingSite></Primer><Primer recentID="2" name="KROL6 Seq_pEX_1a" sequence="gttttcccagtcacgacgtt"><BindingSite location="6031-6050" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="gttttcccagtcacgacgtt"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="gttttcccagtcacgacgtt"/></BindingSite></Primer><Primer recentID="3" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac"><BindingSite location="1003-1027" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="1003-1027" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="1003-1027" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="1003-1027" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="4" name="KROL17 IntpEX" sequence="gataacaatttcacacaggaaacagc"><BindingSite location="7206-7231" boundStrand="1" annealedBases="gataacaatttcacacaggaaacagc" meltingTemperature="57"><Component hybridizedRange="7206-7231" bases="gataacaatttcacacaggaaacagc"/></BindingSite><BindingSite simplified="1" location="7206-7231" boundStrand="1" annealedBases="gataacaatttcacacaggaaacagc" meltingTemperature="57"><Component hybridizedRange="7206-7231" bases="gataacaatttcacacaggaaacagc"/></BindingSite></Primer><Primer recentID="5" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac"><BindingSite location="935-961" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="935-961" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="935-961" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="935-961" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="6" name="KROL92 Tn_Mar3" sequence="attaagtttatttttcaaagcttgaagg"><BindingSite location="870-897" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="870-897" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite><BindingSite simplified="1" location="870-897" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="870-897" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite></Primer><Primer recentID="7" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7228-7247" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="7228-7247" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7228-7247" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="7228-7247" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="8" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="9" name="P824 DFPI_FR2F2" sequence="atatGCGGCCGCTTAAcgccaataacacaatgtttatacagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6393-6430" boundStrand="0" annealedBases="GCGGCCGCTTAAcgccaataacacaatgtttatacagc" meltingTemperature="68"><Component bases="atat"/><Component hybridizedRange="6393-6430" bases="GCGGCCGCTTAAcgccaataacacaatgtttatacagc"/></BindingSite><BindingSite simplified="1" location="6393-6430" boundStrand="0" annealedBases="GCGGCCGCTTAAcgccaataacacaatgtttatacagc" meltingTemperature="68"><Component bases="atat"/><Component hybridizedRange="6393-6430" bases="GCGGCCGCTTAAcgccaataacacaatgtttatacagc"/></BindingSite></Primer><Primer recentID="10" name="P825 DFPI_FR2R2" sequence="atatGGTACCaaagcaaaagctttagctttaataattgc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7145-7179" boundStrand="1" annealedBases="GGTACCaaagcaaaagctttagctttaataattgc" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="7145-7179" bases="GGTACCaaagcaaaagctttagctttaataattgc"/></BindingSite><BindingSite simplified="1" location="7145-7179" boundStrand="1" annealedBases="GGTACCaaagcaaaagctttagctttaataattgc" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="7145-7179" bases="GGTACCaaagcaaaagctttagctttaataattgc"/></BindingSite></Primer><Primer recentID="11" name="P823 DFPI_FR1R2" sequence="atatGCGGCCGCtgctatcattgtctacttaattagaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6365-6400" boundStrand="1" annealedBases="GCGGCCGCtgctatcattgtctacttaattagaaca" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="6365-6400" bases="GCGGCCGCtgctatcattgtctacttaattagaaca"/></BindingSite><BindingSite simplified="1" location="6365-6400" boundStrand="1" annealedBases="GCGGCCGCtgctatcattgtctacttaattagaaca" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="6365-6400" bases="GCGGCCGCtgctatcattgtctacttaattagaaca"/></BindingSite></Primer><Primer recentID="12" name="P822 DFPI_FR1F2" sequence="atatCCTGCAGGatatcctagaaaccttagctcctaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6082-6115" boundStrand="0" annealedBases="CCTGCAGGatatcctagaaaccttagctcctaac" meltingTemperature="63"><Component bases="atat"/><Component hybridizedRange="6082-6115" bases="CCTGCAGGatatcctagaaaccttagctcctaac"/></BindingSite><BindingSite simplified="1" location="6082-6115" boundStrand="0" annealedBases="CCTGCAGGatatcctagaaaccttagctcctaac" meltingTemperature="63"><Component bases="atat"/><Component hybridizedRange="6082-6115" bases="CCTGCAGGatatcctagaaaccttagctcctaac"/></BindingSite></Primer></Primers>
  g<Notes>
<UUID>f3d3f6e7-90c8-4768-970c-d320141b22df</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:21:10">2018.7.9</Created>
<LastModified UTC="19:42:10">2019.2.4</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         Unique 6+ Cutters                                                                                            d     CHF  d              E              Y? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
