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h38\&LN  b!	
r{8e Z~ m E
8o	n
ER1_p  CZKOE;'*#&[* d   _tXdhvDhO~"*>m 
 a' J  2 H+	
/
@=u?|<;C:m' / E   Lw	
^

J`p.38P
IWi.  l7		
dRt[;b.m!Gk.C% 2  RRj
M>*NV4bw& ZvfH?
Sy..z 7dtW;M,    !8FiN	gq5[zFex$M}z  l `n	DNc}D:="Z^o(   1kK6i	


-B2O-l	jVhv$l=  u>h5m	Rpy-EAdyc1 9VkL<inuLyh   Nstst#0189l				uvwx$,MNO@4YZcwx0[\78GU`}fghqz{F u$2{l:XH	P		"%Pu~5[S]Ha~MbN|>&   Oy,b&t+L			
 m_q2r$ A%	Mm;	##vNT{b4J}5 v3#;U	I	Q		r]6\HI;F6NcU* 6|4Y
_B#^bO?P0 {;I}x
9YiQM&Q&#f JU\c&,A
!rgq( <   -;]2M	
tn`	x*A@WZ}' x Pz'u
#N

iRZI-t3s2   e			-#s# BhN>D^
_U|c5) hO
a BRq(			f$K# R <	

$"0	Ok'. G'&9d3njo	
R

5k7w(F
(@rP0c~ kR		M t6JGi,}( ?w4$rGVcJ		v
	s*!]i>dlo	. V<PE	4	:`^GIN<kO " b0	mz77x]VQ! 
ICZ	 	c


(C g^A@$ B mx$				Sz_>QT<  _75O<e	6	@	c	

ntU`<bi|2mISA$cPs .X}6df ,KQ#  # y
[]b[Nt>\4 p<H~>			Y
 d:FZjR''>Cm'-_8$@g  ,z^$&8RV<`"  $|!NT{JGzj. PF^ | 5]0'-		=}	X  KEB	#Jh_ Udz	
-|"}  ?QVtlCsVr! = `  
'u	
>o$
EXb'  dy.<^03N	

wpay+ TNX 2	a			&YrA[~   	ulBV ) 6 (u$h

+j4h0.X" y  #O
:Jnu4Dpt|3B\z  {vI[w QZnSWH  K ]o$sd9L  Er f	s
3q 9   ,u			.}tS  d'g@ C	`3{ GeGZjV1!# 8 o     ci-?&EA }d6*v# + r ;*OS	8
H
'_K^q  iPr)	j
8I3P<n 2F( j  q![S	9		g+YL(v`L<9  *_b1:@ENh	

x^iD>g.ag =.Cx				
1%z G-T%;E6Xl8:% S =	
e
VUo1jPlnr8  KX5






*'/=s8UY|?S01B9 "	 
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Ici#
n(V"  w4S


-l|)(C!3/$  Heo\	p
6X058xq)A!& \  'jk
~lpCHPn%sCd-  (:{pq	

[5GQZ1D U lS		
`I  C	2N!VneKJj 0Wgu!)% D   c;M7aH -~  YE	
M-||]D x+z%sWK		Q
!ot+DL^16FTjepymt
/ @~5LH|w

XA"G%5"T" ; w :%d	M

ssw@?frVp  Q N8=QnQ
C/6'q( ja	s =w+  WB	
		5	?		
;alHR@RJ=~PS@r*   "  oSF			%U_O[l,^7E?] { 4,{k<B|U^W  _  x&/t1	=XqDS Ymg
*3VWvAy  c\nc8p8KR   b,7
@20u9  i  )p0?ZDg	8	


7w]XhmKC_y-/  J!		d





)H:bDWkmA-  iz[

}ZW/o~4~4$B C B	_1bH:fU`6 X}*9y	L


r
n@{r?e$U% ! nM7R						
$.r5?&pR6D\  l%W)jAT@R
JF    (ao+>yf7	




|v?Va.n}W3VJjBl/t/ 86Q	A	
q	a#Gm?-qT>%d5Q	h     /`P=x23vw 	




opKLMuF
-=>cdjklm*+,_nopU=k3 04ax	d			

,1 BNe[`xF#+83^w  		2,9M-f0 }/h Y!5b	

!CO_fuaG$!-L% f 1y

)7F\ey,o_x$ *~@Y	e	-IgKQ4R;  	A
z
8u?&.g 5[mnc\O-]  	\^5:*," $ V	3KebwZ[N 1 C0	#
-
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;P=UvH(?"(.Ah* qi!ZI6?	
eg.M9D`9J' f="c<&		"D[k`Sg (b%:
%*{! S~p		!GnLF   ;c	


%AWhz= 2_z}	*Sap&  g  -{g>


'8DkG] )Kp- 
:$		9;%fY4`y  AUZ	/G<X  +D}O){k%JS_$ %\K
.'RQ56 "|Hf		hLR(b 6Y^
1
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>1nEh  .1-		

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wYGQv~
/5 L 64n6F	q`8 Lj	B]KP!~+a^ %\vyoCd<xi  OC$f
.> +~-o WJ	 .A+ - V	

]_z}~k;+  Ye{		!
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m[!;Dt\*s&   @4f1EyOTI
#1Gt$ % #t+wL(QcDuWZ* > 1}	$

'Vw
LU^bx0pz@#iu' G a a (
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
tbv/RK;aK  z	8hq"z:EO  T["1@
,Y gFg'			!^& >Uqb3	u
l)F5+\k|' -N~#d	b			~
'ms5Thcf+]  MG =yX
Z#E\aZ&-;=sB  T	" `			vo#kMUWa ( 5  tHmCoG  Jq+t	r
r * 7  )Rd%G
iJ]z '  <-^Mw		i
O*01='uEf( W


&,Tnhr5TXi{>R/m078U$   v
kBGO'pY 2C  
dI# v ${~	CKv05ESq4{ `m	P
TQ}]v?q ^ z  3w.s+;bUo{VuC#  h  h	

69\ 3x   |)K
qo#I1 '.nw1	


0J"El,)^*7.4]v!  |)g? 
(^EHJLe Ulm	vYZ%+$ BRo;%	f<T9KEIz   =9		:gXJ3 C[	&Q'5a   %?-U^	
FPdM $Bi	p *e	= V 
y*j'  "L*sJ[	%P0t :SH	FY#  P|} z&K@T]rTw%Y  !g!: ,E~LTF}	t
l4e,~r\ ' 4  s*s	jT`# ]VLv	N/SNI6y7BT u v-lr	~BTRu  &f	

/!]+(63\d T<	P8XI*  >#~ITh		
i) S\$i e	O7MS)Ac }g	(h  0f  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                       1                                                      
      	                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        	                                                                                                                        	                                                                                                                                         +               	                                                                                                               	                              
   	                     	      	                        *               	                                                	                     
                                       '   '      .   !         #            u      $   _   `   *         6   l                                                                                                                                                                            1                          
  	                                                                                                                                                                                                                                                                    	                                              	                                                       +     	                                         	          
 	       	  	        *     	                	       
             ' '  . !   #    u  $ _ ` *   6 l         &      D
u I	*r)*F"/1 #q
^OR4cccc9XvS 'OR[dS<;9&Rx;B

g
 5I&Zc eDkDN	 y	(y	( r~H*	9
wQ{3^aN ury	C~|o
oo
lo
b	+r.	O$^ sTCruCw rArW	~ 6<d,}|S\y*L$]EO<'		o
oof&		~=J4w $QF
yKmqu2EOSDX\7
	~,"xh6t	mXDMj u.  
I kOdn(~
kl(o

F g  f"\cK&			!eUg A<$EO .
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o1dixb6 #zii hp YZ5		8	
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consecutiveTranslationNumbering="1"><Segment range="8354-8375" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="74" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="73" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8309-8315" color="#ffffff" type="standard"/><Segment range="8316-8333" color="#ffffff" type="standard"/><Segment name="-35" range="8334-8339" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="72" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="71" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="70" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="69" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="68" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8663-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="67" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="66" name="SacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="65" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="64" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="63" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="62" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" 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pEX18KanSeq2F"/></Q></Feature><Feature recentID="54" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="53" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="52" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="51" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6524-7981" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3951071" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0702"/></Q></Feature><Feature recentID="50" name="FTL_0702" directionality="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7988" maxFusedRunOn="7988"><Segment range="6519-7981" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="YP_513453.1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="inference"><V predef="ab initio prediction"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="db_xref"><V text="89256091" predef="GI"/><V text="3951071" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0702"/></Q></Feature><Feature recentID="49" name="P364 FTL0702cmp_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6524-6550" color="#a020f0" type="standard"/><Q name="note"><V text="P364 FTL0702cmp_F"/></Q></Feature><Feature recentID="48" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6588-6617" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P703 CkPriM_mtip1F"/></Q></Feature><Feature recentID="47" name="glyA" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6246" color="#a6acb3" type="standard"/><Q name="gene"><V text="glyA"/></Q><Q name="db_xref"><V text="3951072" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0703"/></Q></Feature><Feature recentID="46" name="glyA" directionality="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6265" maxFusedRunOn="6265"><Segment range="6113-6246" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="EC_number"><V text="2.1.2.1"/></Q><Q name="protein_id"><V text="YP_513454.1"/></Q><Q name="product"><V text="serine hydroxymethyltransferase"/></Q><Q name="gene"><V text="glyA"/></Q><Q name="inference"><V bool="1" text="2004" predef="profile"/></Q><Q name="note"><V text="catalyzes the reaction of glycine with 5,10-methylenetetrahydrofolate to form L-serine and tetrahydrofolate"/><V text="/dnas_title=serine hydroxymethyltransferase"/></Q><Q name="db_xref"><V text="89256092" predef="GI"/><V text="3951072" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0703"/></Q></Feature><Feature recentID="45" name="RPp4" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6506-6532" color="#a6acb3" type="standard"/><Q name="note"><V text="RPp4"/></Q></Feature><Feature recentID="44" name="PRP12" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6345-6374" color="#a020f0" type="standard"/><Q name="note"><V text="PRP12"/></Q></Feature><Feature recentID="43" name="200 bp max AT content (91%)" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6306-6505" color="#a6acb3" type="standard"/><Q name="note"><V text="200 bp max AT content (91%)"/></Q></Feature><Feature recentID="42" name="150 bp max AT content (93.3%)" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6356-6505" color="#a6acb3" type="standard"/><Q name="note"><V text="150 bp max AT content (93.3%)"/></Q></Feature><Feature recentID="41" name="100 bp max AT content (95%)" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6385-6484" color="#a6acb3" type="standard"/><Q name="note"><V text="100 bp max AT content (95%)"/></Q></Feature><Feature recentID="40" name="promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6474-6474" color="#ffffff" type="standard"/><Q name="note"><V text="Potential TSS"/></Q></Feature><Feature recentID="39" name="Potential -10 element" type="-10_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6462-6467" color="#a6acb3" type="standard"/><Q name="note"><V text="Potential -10 element"/></Q></Feature><Feature recentID="38" name="Potential -35 element" type="-35_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6440-6445" color="#a6acb3" type="standard"/><Q name="note"><V text="Potential -35 element"/></Q></Feature><Feature recentID="37" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6345-6374" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P431 P_FTL0702_F"/></Q></Feature><Feature recentID="36" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6473-6506" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P434 FTL0702cmp_F2"/></Q></Feature><Feature recentID="35" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6216-6237" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P478 RT_up0702-1_F"/></Q></Feature><Feature recentID="34" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6223-6249" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P480 RT_up0702-2_F"/></Q></Feature><Feature recentID="33" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6151-6175" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P482 RT_up0702-3_F"/></Q></Feature><Feature recentID="32" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6107-6139" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P691 PriM_mpk_F1"/></Q></Feature><Feature recentID="31" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-8239" color="#ffffff" type="standard"/><Q name="mol_type"><V predef="genomic DNA"/></Q><Q name="strain"><V text="LVS"/></Q><Q name="note"><V text="/dnas_title=pKL118 pEX_PriM_unst1"/></Q><Q name="db_xref"><V text="119857" predef="taxon"/></Q><Q name="sub_species"><V text="holarctica"/></Q><Q name="organism"><V text="Francisella tularensis subsp. holarctica"/></Q></Feature><Feature recentID="30" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7061-7087" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P704 CkPriM_mtip1R"/></Q></Feature><Feature recentID="29" name="P367 FTL0702seqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6831-6855" color="#a020f0" type="standard"/><Q name="note"><V text="P367 FTL0702seqF1"/></Q></Feature><Feature recentID="28" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6928-6954" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P429 FTL0702_VSVG_2R"/></Q></Feature><Feature recentID="27" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6856-6883" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P700 PriM_mtip1_R1"/></Q></Feature><Feature recentID="26" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6908-6933" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P701 PriM_mtip1_F2"/></Q></Feature><Feature recentID="25" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6856-6883" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P706 PriM_mtip2_R1"/></Q></Feature><Feature recentID="24" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6923-6949" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P705 PriM_mtip2_F2"/></Q></Feature><Feature recentID="23" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7602-7626" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="22" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7592-7614" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P698 Ck_PriM_mpk1R"/></Q></Feature><Feature recentID="21" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7116-7135" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="20" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7214-7235" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="19" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7333-7354" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="18" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7399-7418" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="17" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7439-7465" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="16" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7116-7139" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature><Feature recentID="15" name="FTL_0701" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="8046-8239" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3951070" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0701"/></Q></Feature><Feature recentID="14" name="FTL_0701" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="527" hitsStopCodon="1"><Segment range="8041-8234" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="3"/></Q><Q name="protein_id"><V text="YP_513452.1"/></Q><Q name="product"><V text="FAD binding family protein"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="note"><V text="/dnas_title=FAD binding family protein"/></Q><Q name="db_xref"><V text="89256090" predef="GI"/><V text="3951070" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0701"/></Q></Feature><Feature recentID="13" name="P329 SP_D0702-R" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8072-8099" color="#a020f0" type="standard"/><Q name="note"><V text="P329 SP_D0702-R"/></Q></Feature><Feature recentID="12" name="PRP11" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8106-8136" color="#a020f0" type="standard"/><Q name="note"><V text="PRP11"/></Q></Feature><Feature recentID="11" name="RPp7" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7972-8000" color="#a6acb3" type="standard"/><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="10" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7982-8011" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P427 FTL0702_VSVG_1R"/></Q></Feature><Feature recentID="9" name="P365 FTL0702cmp_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7951-7978" color="#a020f0" type="standard"/><Q name="note"><V text="P365 FTL0702cmp_R"/></Q></Feature><Feature recentID="8" name="P368 FTL0702seqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7671-7699" color="#a020f0" type="standard"/><Q name="note"><V text="P368 FTL0702seqR1"/></Q></Feature><Feature recentID="7" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7951-7978" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P428 FTL0702_VSVG_2F"/></Q></Feature><Feature recentID="6" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7954-7981" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P433 FTL0702cmp_R2"/></Q></Feature><Feature recentID="5" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7749-7771" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P437 RT_FTL0702-2F"/></Q></Feature><Feature recentID="4" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7854-7875" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P438 RT_FTL0702-2R"/></Q></Feature><Feature recentID="3" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7894-7918" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P702 PriM_mtip1_R2"/></Q></Feature><Feature recentID="2" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8212-8245" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P694 PriM_mpk_R2"/></Q></Feature><Feature recentID="1" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7159-7205" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P707 PriM_unst1_R1"/></Q></Feature><Feature recentID="0" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7144-7188" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  @<Notes>
<UUID>564554f1-0662-45ed-a415-480ccaa523af</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:37:10">2018.7.2</Created>
<LastModified UTC="16:1:38">2018.7.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  .e       H        HfVVkXYխT4If5`EV
yu8{SVbfS^8BEgDQF]vWREiZTST]YU	VeNDUfUf^%`PɪXfSijV4ytS]جD`vMQ9my.Pו4y]ޝ^yǥ$UY:Qpmj!NNm}RtViՕTUQޞVfqZCxSNucZFY
gP-YԒ`hԔ8dJ栕XؖNUd[JT4ѓׯREIПf          
  C  E  J  N  =  D  G  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="26"><Feature recentID="25" name="P708 PriM_unst1_F1" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="591-615" color="#50ff50" type="standard"/><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature><Feature recentID="24" name="P707 PriM_unst1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="554-580" color="#50ff50" type="standard"/><Q name="note"><V text="P707 PriM_unst1_R1"/></Q></Feature><Feature recentID="23" name="P702 PriM_mtip1_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1304-1328" color="#50ff50" type="standard"/><Q name="note"><V text="P702 PriM_mtip1_R2"/></Q></Feature><Feature recentID="22" name="P698 Ck_PriM_mpk1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1002-1024" color="#50ff50" type="standard"/><Q name="note"><V text="P698 Ck_PriM_mpk1R"/></Q></Feature><Feature recentID="21" name="P693 PriM_mpk_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="612-640" color="#50ff50" type="standard"/><Q name="note"><V text="P693 PriM_mpk_F2"/></Q></Feature><Feature recentID="20" name="P692 PriM_mpk_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="561-587" color="#50ff50" type="standard"/><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="19" name="P444 pKL75_F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="849-875" color="#50ff50" type="standard"/><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="18" name="P440 RT_FTL0702-3R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="809-828" color="#50ff50" type="standard"/><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="17" name="P439 RT_FTL0702-3F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="743-764" color="#50ff50" type="standard"/><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="16" name="P438 RT_FTL0702-2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1264-1285" color="#50ff50" type="standard"/><Q name="note"><V text="P438 RT_FTL0702-2R"/></Q></Feature><Feature recentID="15" name="P437 RT_FTL0702-2F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="1159-1181" color="#50ff50" type="standard"/><Q name="note"><V text="P437 RT_FTL0702-2F"/></Q></Feature><Feature recentID="14" name="P436 RT_FTL0702-1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="624-645" color="#50ff50" type="standard"/><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="13" name="P411 FTL0702sp_2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1012-1036" color="#50ff50" type="standard"/><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="12" name="P368 FTL0702seqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1081-1109" color="#8080ff" type="standard"/><Q name="note"><V text="P368 FTL0702seqR1"/></Q></Feature><Feature recentID="11" name="hypothetical protein" directionality="1" translationMW="48605.96" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="27" maxRunOn="1352" maxFusedRunOn="1352"><Segment range="11-1342" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="11-1342" color="#00e600" type="standard"/></Feature><Feature recentID="9" name="P705 PriM_mtip2_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="333-359" color="#50ff50" type="standard"/><Q name="note"><V text="P705 PriM_mtip2_F2"/></Q></Feature><Feature recentID="8" name="P706 PriM_mtip2_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="266-293" color="#50ff50" type="standard"/><Q name="note"><V text="P706 PriM_mtip2_R1"/></Q></Feature><Feature recentID="7" name="P704 CkPriM_mtip1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="471-497" color="#50ff50" type="standard"/><Q name="note"><V text="P704 CkPriM_mtip1R"/></Q></Feature><Feature recentID="6" name="P703 CkPriM_mtip1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="11-27" color="#50ff50" type="standard"/><Q name="note"><V text="P703 CkPriM_mtip1F"/></Q></Feature><Feature recentID="5" name="P701 PriM_mtip1_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="318-343" color="#50ff50" type="standard"/><Q name="note"><V text="P701 PriM_mtip1_F2"/></Q></Feature><Feature recentID="4" name="P700 PriM_mtip1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="266-293" color="#50ff50" type="standard"/><Q name="note"><V text="P700 PriM_mtip1_R1"/></Q></Feature><Feature recentID="3" name="P697 Ck_PriM_mpk1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="526-549" color="#50ff50" type="standard"/><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature><Feature recentID="2" name="P435 RT_FTL0702-1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="526-545" color="#50ff50" type="standard"/><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="1" name="P429 FTL0702_VSVG_2R" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="338-364" color="#50ff50" type="standard"/><Q name="note"><V text="P429 FTL0702_VSVG_2R"/></Q></Feature><Feature recentID="0" name="P367 FTL0702seqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="241-265" color="#8080ff" type="standard"/><Q name="note"><V text="P367 FTL0702seqF1"/></Q></Feature></Features>
  k<?xml version="1.0"?><Primers nextValidID="5"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P818 PriM_mpk_F2" sequence="atatGGATCCaattgtaaatgaatctgttactaaatattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-39" boundStrand="0" annealedBases="atatGGATCCaattgtaaatgaatctgttactaaatattc" meltingTemperature="57"><Component hybridizedRange="0-39" bases="atatGGATCCaattgtaaatgaatctgttactaaatattc"/></BindingSite><BindingSite simplified="1" location="0-39" boundStrand="0" annealedBases="atatGGATCCaattgtaaatgaatctgttactaaatattc" meltingTemperature="57"><Component hybridizedRange="0-39" bases="atatGGATCCaattgtaaatgaatctgttactaaatattc"/></BindingSite></Primer><Primer recentID="1" name="P707 PriM_unst1_R1" sequence="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="553-599" boundStrand="1" annealedBases="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" meltingTemperature="68"><Component hybridizedRange="553-599" bases="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag"/></BindingSite><BindingSite simplified="1" location="553-599" boundStrand="1" annealedBases="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" meltingTemperature="68"><Component hybridizedRange="553-599" bases="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag"/></BindingSite></Primer><Primer recentID="2" name="P821 PriM_mtip1_R3" sequence="atatGGTACCgtaactttggtattagttctgatactg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="856-882" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="856-882" bases="gtaactttggtattagttctgatactg"/></BindingSite><BindingSite simplified="1" location="856-882" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="856-882" bases="gtaactttggtattagttctgatactg"/></BindingSite></Primer><Primer recentID="3" name="P819 PriM_mpk_R2" sequence="atatGGTACCtgtaaagccaaatatactttctcttcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1315-1351" boundStrand="1" annealedBases="atatGGTACCtgtaaagccaaatatactttctcttcg" meltingTemperature="61"><Component hybridizedRange="1315-1351" bases="atatGGTACCtgtaaagccaaatatactttctcttcg"/></BindingSite><BindingSite simplified="1" location="1315-1351" boundStrand="1" annealedBases="atatGGTACCtgtaaagccaaatatactttctcttcg" meltingTemperature="61"><Component hybridizedRange="1315-1351" bases="atatGGTACCtgtaaagccaaatatactttctcttcg"/></BindingSite></Primer><Primer recentID="4" name="P708 PriM_unst1_F1" sequence="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="570-614" boundStrand="0" annealedBases="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" meltingTemperature="68"><Component hybridizedRange="570-614" bases="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg"/></BindingSite><BindingSite simplified="1" location="570-614" boundStrand="0" annealedBases="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" meltingTemperature="68"><Component hybridizedRange="570-614" bases="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg"/></BindingSite></Primer></Primers>
  e<Notes>
<UUID>5c4f04be-03e5-4bd6-8e04-af427f273645</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:32">2018.7.6</Created>
<LastModified UTC="16:1:57">2018.7.6</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  P        X        Xص:Ky$ojV!x:MfUAJff1NjAYꤩ:+MyZOz*binۺ&~J:fvj:}u_9jfꅍIۋijItkjuof       	           M  T  W  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  A<?xml version="1.0"?><Features nextValidID="15"><Feature recentID="14" name="P708 PriM_unst1_F1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="1-10" color="#50ff50" type="standard"/><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature><Feature recentID="13" name="P707 PriM_unst1_R1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="21-47" color="#50ff50" type="standard"/><Q name="note"><V text="P707 PriM_unst1_R1"/></Q></Feature><Feature recentID="12" name="P705 PriM_mtip2_F2" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="242-268" color="#50ff50" type="standard"/><Q name="note"><V text="P705 PriM_mtip2_F2"/></Q></Feature><Feature recentID="11" name="P706 PriM_mtip2_R1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="308-335" color="#50ff50" type="standard"/><Q name="note"><V text="P706 PriM_mtip2_R1"/></Q></Feature><Feature recentID="10" name="P704 CkPriM_mtip1R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="104-130" color="#50ff50" type="standard"/><Q name="note"><V text="P704 CkPriM_mtip1R"/></Q></Feature><Feature recentID="9" name="P703 CkPriM_mtip1F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="574-590" color="#50ff50" type="standard"/><Q name="note"><V text="P703 CkPriM_mtip1F"/></Q></Feature><Feature recentID="8" name="P701 PriM_mtip1_F2" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="258-283" color="#50ff50" type="standard"/><Q name="note"><V text="P701 PriM_mtip1_F2"/></Q></Feature><Feature recentID="7" name="P700 PriM_mtip1_R1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="308-335" color="#50ff50" type="standard"/><Q name="note"><V text="P700 PriM_mtip1_R1"/></Q></Feature><Feature recentID="6" name="P697 Ck_PriM_mpk1F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="52-75" color="#50ff50" type="standard"/><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature><Feature recentID="5" name="P692 PriM_mpk_R1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="14-40" color="#50ff50" type="standard"/><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="4" name="P435 RT_FTL0702-1F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="56-75" color="#50ff50" type="standard"/><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="3" name="P429 FTL0702_VSVG_2R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="237-263" color="#50ff50" type="standard"/><Q name="note"><V text="P429 FTL0702_VSVG_2R"/></Q></Feature><Feature recentID="2" name="P367 FTL0702seqF1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="336-360" color="#8080ff" type="standard"/><Q name="note"><V text="P367 FTL0702seqF1"/></Q></Feature><Feature recentID="1" name="hypothetical protein" directionality="2" translationMW="21718.23" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="27"><Segment range="1-590" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="FTL_0702" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="1-590" color="#00e600" type="standard"/></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P818 PriM_mpk_F2" sequence="atatGGATCCaattgtaaatgaatctgttactaaatattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="560-599" boundStrand="1" annealedBases="atatGGATCCaattgtaaatgaatctgttactaaatattc" meltingTemperature="57"><Component hybridizedRange="560-599" bases="atatGGATCCaattgtaaatgaatctgttactaaatattc"/></BindingSite><BindingSite simplified="1" location="560-599" boundStrand="1" annealedBases="atatGGATCCaattgtaaatgaatctgttactaaatattc" meltingTemperature="57"><Component hybridizedRange="560-599" bases="atatGGATCCaattgtaaatgaatctgttactaaatattc"/></BindingSite></Primer><Primer recentID="1" name="P707 PriM_unst1_R1" sequence="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-46" boundStrand="0" annealedBases="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" meltingTemperature="68"><Component hybridizedRange="0-46" bases="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag"/></BindingSite><BindingSite simplified="1" location="0-46" boundStrand="0" annealedBases="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" meltingTemperature="68"><Component hybridizedRange="0-46" bases="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag"/></BindingSite></Primer></Primers>
  g<Notes>
<UUID>6ee12a74-f3a8-41e0-8482-c5a809210aca</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="15:59:36">2018.7.6</Created>
<LastModified UTC="15:59:36">2018.7.6</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  L                f	=Ypz(9[d^d)N8iکd^m蕫kOy葚]I\&y_(nZ6N:ҋ>ZTfYjIHItꩨdjIzi+xiNNVDS],JxHMْ(yGd$lkX4hbQyص          
            <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="16"><Feature recentID="15" name="P708 PriM_unst1_F1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="738-762" color="#50ff50" type="standard"/><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature><Feature recentID="14" name="P707 PriM_unst1_R1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="773-782" color="#50ff50" type="standard"/><Q name="note"><V text="P707 PriM_unst1_R1"/></Q></Feature><Feature recentID="13" name="P702 PriM_mtip1_R2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="25-49" color="#50ff50" type="standard"/><Q name="note"><V text="P702 PriM_mtip1_R2"/></Q></Feature><Feature recentID="12" name="P698 Ck_PriM_mpk1R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="329-351" color="#50ff50" type="standard"/><Q name="note"><V text="P698 Ck_PriM_mpk1R"/></Q></Feature><Feature recentID="11" name="P693 PriM_mpk_F2" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="713-741" color="#50ff50" type="standard"/><Q name="note"><V text="P693 PriM_mpk_F2"/></Q></Feature><Feature recentID="10" name="P692 PriM_mpk_R1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="766-782" color="#50ff50" type="standard"/><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="9" name="P444 pKL75_F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="478-504" color="#50ff50" type="standard"/><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="8" name="P440 RT_FTL0702-3R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="525-544" color="#50ff50" type="standard"/><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="7" name="P439 RT_FTL0702-3F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="589-610" color="#50ff50" type="standard"/><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="6" name="P438 RT_FTL0702-2R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="68-89" color="#50ff50" type="standard"/><Q name="note"><V text="P438 RT_FTL0702-2R"/></Q></Feature><Feature recentID="5" name="P437 RT_FTL0702-2F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="172-194" color="#50ff50" type="standard"/><Q name="note"><V text="P437 RT_FTL0702-2F"/></Q></Feature><Feature recentID="4" name="P436 RT_FTL0702-1R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="708-729" color="#50ff50" type="standard"/><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="3" name="P411 FTL0702sp_2R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="317-341" color="#50ff50" type="standard"/><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="2" name="P368 FTL0702seqR1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="244-272" color="#8080ff" type="standard"/><Q name="note"><V text="P368 FTL0702seqR1"/></Q></Feature><Feature recentID="1" name="hypothetical protein" directionality="2" translationMW="27881.90" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="214" maxRunOn="1" maxFusedRunOn="1"><Segment range="11-782" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="FTL_0702" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="11-782" color="#00e600" type="standard"/></Feature></Features>
  2<?xml version="1.0"?><Primers nextValidID="3"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P821 PriM_mtip1_R3" sequence="atatGGTACCgtaactttggtattagttctgatactg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="469-495" boundStrand="0" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="469-495" bases="gtaactttggtattagttctgatactg"/></BindingSite><BindingSite simplified="1" location="469-495" boundStrand="0" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="469-495" bases="gtaactttggtattagttctgatactg"/></BindingSite></Primer><Primer recentID="1" name="P819 PriM_mpk_R2" sequence="atatGGTACCtgtaaagccaaatatactttctcttcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-36" boundStrand="0" annealedBases="atatGGTACCtgtaaagccaaatatactttctcttcg" meltingTemperature="61"><Component hybridizedRange="0-36" bases="atatGGTACCtgtaaagccaaatatactttctcttcg"/></BindingSite><BindingSite simplified="1" location="0-36" boundStrand="0" annealedBases="atatGGTACCtgtaaagccaaatatactttctcttcg" meltingTemperature="61"><Component hybridizedRange="0-36" bases="atatGGTACCtgtaaagccaaatatactttctcttcg"/></BindingSite></Primer><Primer recentID="2" name="P708 PriM_unst1_F1" sequence="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="737-781" boundStrand="1" annealedBases="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" meltingTemperature="68"><Component hybridizedRange="737-781" bases="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg"/></BindingSite><BindingSite simplified="1" location="737-781" boundStrand="1" annealedBases="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" meltingTemperature="68"><Component hybridizedRange="737-781" bases="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg"/></BindingSite></Primer></Primers>
  e<Notes>
<UUID>0bddf9a8-14c3-4655-9fe5-f1da1fc31f2b</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:12">2018.7.6</Created>
<LastModified UTC="16:0:12">2018.7.6</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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  n<?xml version="1.0"?><Features nextValidID="79"><Feature recentID="78" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8285-8301" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="77" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8261-8277" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="76" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="75" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8354-8375" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="74" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="73" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8309-8315" color="#ffffff" type="standard"/><Segment range="8316-8333" color="#ffffff" type="standard"/><Segment name="-35" range="8334-8339" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="72" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="71" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="70" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="69" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="68" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8663-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="67" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="66" name="SacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="65" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="64" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="63" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="62" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="61" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="60" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8272-8297" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="59" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8294-8313" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="58" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="57" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="56" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="55" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="54" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="53" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="52" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="51" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6524-7981" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3951071" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0702"/></Q></Feature><Feature recentID="50" name="FTL_0702" directionality="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7988" maxFusedRunOn="7988"><Segment range="6519-7981" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="YP_513453.1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="inference"><V predef="ab initio prediction"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="db_xref"><V text="89256091" predef="GI"/><V text="3951071" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0702"/></Q></Feature><Feature recentID="49" name="P364 FTL0702cmp_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6524-6550" color="#a020f0" type="standard"/><Q name="note"><V text="P364 FTL0702cmp_F"/></Q></Feature><Feature recentID="48" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6588-6617" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P703 CkPriM_mtip1F"/></Q></Feature><Feature recentID="47" name="glyA" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6246" color="#a6acb3" type="standard"/><Q name="gene"><V text="glyA"/></Q><Q name="db_xref"><V text="3951072" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0703"/></Q></Feature><Feature recentID="46" name="glyA" directionality="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6265" maxFusedRunOn="6265"><Segment range="6113-6246" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="EC_number"><V text="2.1.2.1"/></Q><Q name="protein_id"><V text="YP_513454.1"/></Q><Q name="product"><V text="serine hydroxymethyltransferase"/></Q><Q name="gene"><V text="glyA"/></Q><Q name="inference"><V bool="1" text="2004" predef="profile"/></Q><Q name="note"><V text="catalyzes the reaction of glycine with 5,10-methylenetetrahydrofolate to form L-serine and tetrahydrofolate"/><V text="/dnas_title=serine hydroxymethyltransferase"/></Q><Q name="db_xref"><V text="89256092" predef="GI"/><V text="3951072" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0703"/></Q></Feature><Feature recentID="45" name="RPp4" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6506-6532" color="#a6acb3" type="standard"/><Q name="note"><V text="RPp4"/></Q></Feature><Feature recentID="44" name="PRP12" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6345-6374" color="#a020f0" type="standard"/><Q name="note"><V text="PRP12"/></Q></Feature><Feature recentID="43" name="200 bp max AT content (91%)" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6306-6505" color="#a6acb3" type="standard"/><Q name="note"><V text="200 bp max AT content (91%)"/></Q></Feature><Feature recentID="42" name="150 bp max AT content (93.3%)" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6356-6505" color="#a6acb3" type="standard"/><Q name="note"><V text="150 bp max AT content (93.3%)"/></Q></Feature><Feature recentID="41" name="100 bp max AT content (95%)" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6385-6484" color="#a6acb3" type="standard"/><Q name="note"><V text="100 bp max AT content (95%)"/></Q></Feature><Feature recentID="40" name="promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6474-6474" color="#ffffff" type="standard"/><Q name="note"><V text="Potential TSS"/></Q></Feature><Feature recentID="39" name="Potential -10 element" type="-10_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6462-6467" color="#a6acb3" type="standard"/><Q name="note"><V text="Potential -10 element"/></Q></Feature><Feature recentID="38" name="Potential -35 element" type="-35_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6440-6445" color="#a6acb3" type="standard"/><Q name="note"><V text="Potential -35 element"/></Q></Feature><Feature recentID="37" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6345-6374" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P431 P_FTL0702_F"/></Q></Feature><Feature recentID="36" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6473-6506" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P434 FTL0702cmp_F2"/></Q></Feature><Feature recentID="35" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6216-6237" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P478 RT_up0702-1_F"/></Q></Feature><Feature recentID="34" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6223-6249" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P480 RT_up0702-2_F"/></Q></Feature><Feature recentID="33" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6151-6175" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P482 RT_up0702-3_F"/></Q></Feature><Feature recentID="32" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6107-6139" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P691 PriM_mpk_F1"/></Q></Feature><Feature recentID="31" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-8239" color="#ffffff" type="standard"/><Q name="mol_type"><V predef="genomic DNA"/></Q><Q name="strain"><V text="LVS"/></Q><Q name="note"><V text="/dnas_title=pKL118 pEX_PriM_unst1"/></Q><Q name="db_xref"><V text="119857" predef="taxon"/></Q><Q name="sub_species"><V text="holarctica"/></Q><Q name="organism"><V text="Francisella tularensis subsp. holarctica"/></Q></Feature><Feature recentID="30" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7061-7087" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P704 CkPriM_mtip1R"/></Q></Feature><Feature recentID="29" name="P367 FTL0702seqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6831-6855" color="#a020f0" type="standard"/><Q name="note"><V text="P367 FTL0702seqF1"/></Q></Feature><Feature recentID="28" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6928-6954" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P429 FTL0702_VSVG_2R"/></Q></Feature><Feature recentID="27" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6856-6883" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P700 PriM_mtip1_R1"/></Q></Feature><Feature recentID="26" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6908-6933" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P701 PriM_mtip1_F2"/></Q></Feature><Feature recentID="25" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6856-6883" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P706 PriM_mtip2_R1"/></Q></Feature><Feature recentID="24" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6923-6949" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P705 PriM_mtip2_F2"/></Q></Feature><Feature recentID="23" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7602-7626" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="22" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7592-7614" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P698 Ck_PriM_mpk1R"/></Q></Feature><Feature recentID="21" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7116-7135" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="20" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7214-7235" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="19" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7333-7354" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="18" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7399-7418" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="17" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7439-7465" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="16" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7116-7139" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature><Feature recentID="15" name="FTL_0701" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="8046-8239" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3951070" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0701"/></Q></Feature><Feature recentID="14" name="FTL_0701" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="527" hitsStopCodon="1"><Segment range="8041-8234" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="3"/></Q><Q name="protein_id"><V text="YP_513452.1"/></Q><Q name="product"><V text="FAD binding family protein"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="note"><V text="/dnas_title=FAD binding family protein"/></Q><Q name="db_xref"><V text="89256090" predef="GI"/><V text="3951070" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0701"/></Q></Feature><Feature recentID="13" name="P329 SP_D0702-R" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8072-8099" color="#a020f0" type="standard"/><Q name="note"><V text="P329 SP_D0702-R"/></Q></Feature><Feature recentID="12" name="PRP11" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8106-8136" color="#a020f0" type="standard"/><Q name="note"><V text="PRP11"/></Q></Feature><Feature recentID="11" name="RPp7" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7972-8000" color="#a6acb3" type="standard"/><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="10" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7982-8011" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P427 FTL0702_VSVG_1R"/></Q></Feature><Feature recentID="9" name="P365 FTL0702cmp_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7951-7978" color="#a020f0" type="standard"/><Q name="note"><V text="P365 FTL0702cmp_R"/></Q></Feature><Feature recentID="8" name="P368 FTL0702seqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7671-7699" color="#a020f0" type="standard"/><Q name="note"><V text="P368 FTL0702seqR1"/></Q></Feature><Feature recentID="7" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7951-7978" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P428 FTL0702_VSVG_2F"/></Q></Feature><Feature recentID="6" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7954-7981" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P433 FTL0702cmp_R2"/></Q></Feature><Feature recentID="5" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7749-7771" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P437 RT_FTL0702-2F"/></Q></Feature><Feature recentID="4" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7854-7875" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P438 RT_FTL0702-2R"/></Q></Feature><Feature recentID="3" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7894-7918" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P702 PriM_mtip1_R2"/></Q></Feature><Feature recentID="2" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8212-8245" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P694 PriM_mpk_R2"/></Q></Feature><Feature recentID="1" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7159-7205" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P707 PriM_unst1_R1"/></Q></Feature><Feature recentID="0" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7144-7188" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  @<Notes>
<UUID>564554f1-0662-45ed-a415-480ccaa523af</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="12:37:10">2018.7.2</Created>
<LastModified UTC="16:1:38">2018.7.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  .e       H        HfVVkXYխT4If5`EV
yu8{SVbfS^8BEgDQF]vWREiZTST]YU	VeNDUfUf^%`PɪXfSijV4ytS]جD`vMQ9my.Pו4y]ޝ^yǥ$UY:Qpmj!NNm}RtViՕTUQޞVfqZCxSNucZFY
gP-YԒ`hԔ8dJ栕XؖNUd[JT4ѓׯREIПf          
  C  E  J  N  =  D  G  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="26"><Feature recentID="25" name="P708 PriM_unst1_F1" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="591-615" color="#50ff50" type="standard"/><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature><Feature recentID="24" name="P707 PriM_unst1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="554-580" color="#50ff50" type="standard"/><Q name="note"><V text="P707 PriM_unst1_R1"/></Q></Feature><Feature recentID="23" name="P702 PriM_mtip1_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1304-1328" color="#50ff50" type="standard"/><Q name="note"><V text="P702 PriM_mtip1_R2"/></Q></Feature><Feature recentID="22" name="P698 Ck_PriM_mpk1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1002-1024" color="#50ff50" type="standard"/><Q name="note"><V text="P698 Ck_PriM_mpk1R"/></Q></Feature><Feature recentID="21" name="P693 PriM_mpk_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="612-640" color="#50ff50" type="standard"/><Q name="note"><V text="P693 PriM_mpk_F2"/></Q></Feature><Feature recentID="20" name="P692 PriM_mpk_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="561-587" color="#50ff50" type="standard"/><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="19" name="P444 pKL75_F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="849-875" color="#50ff50" type="standard"/><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="18" name="P440 RT_FTL0702-3R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="809-828" color="#50ff50" type="standard"/><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="17" name="P439 RT_FTL0702-3F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="743-764" color="#50ff50" type="standard"/><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="16" name="P438 RT_FTL0702-2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1264-1285" color="#50ff50" type="standard"/><Q name="note"><V text="P438 RT_FTL0702-2R"/></Q></Feature><Feature recentID="15" name="P437 RT_FTL0702-2F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="1159-1181" color="#50ff50" type="standard"/><Q name="note"><V text="P437 RT_FTL0702-2F"/></Q></Feature><Feature recentID="14" name="P436 RT_FTL0702-1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="624-645" color="#50ff50" type="standard"/><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="13" name="P411 FTL0702sp_2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1012-1036" color="#50ff50" type="standard"/><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="12" name="P368 FTL0702seqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1081-1109" color="#8080ff" type="standard"/><Q name="note"><V text="P368 FTL0702seqR1"/></Q></Feature><Feature recentID="11" name="hypothetical protein" directionality="1" translationMW="48605.96" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="27" maxRunOn="1352" maxFusedRunOn="1352"><Segment range="11-1342" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="11-1342" color="#00e600" type="standard"/></Feature><Feature recentID="9" name="P705 PriM_mtip2_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="333-359" color="#50ff50" type="standard"/><Q name="note"><V text="P705 PriM_mtip2_F2"/></Q></Feature><Feature recentID="8" name="P706 PriM_mtip2_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="266-293" color="#50ff50" type="standard"/><Q name="note"><V text="P706 PriM_mtip2_R1"/></Q></Feature><Feature recentID="7" name="P704 CkPriM_mtip1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="471-497" color="#50ff50" type="standard"/><Q name="note"><V text="P704 CkPriM_mtip1R"/></Q></Feature><Feature recentID="6" name="P703 CkPriM_mtip1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="11-27" color="#50ff50" type="standard"/><Q name="note"><V text="P703 CkPriM_mtip1F"/></Q></Feature><Feature recentID="5" name="P701 PriM_mtip1_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="318-343" color="#50ff50" type="standard"/><Q name="note"><V text="P701 PriM_mtip1_F2"/></Q></Feature><Feature recentID="4" name="P700 PriM_mtip1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="266-293" color="#50ff50" type="standard"/><Q name="note"><V text="P700 PriM_mtip1_R1"/></Q></Feature><Feature recentID="3" name="P697 Ck_PriM_mpk1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="526-549" color="#50ff50" type="standard"/><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature><Feature recentID="2" name="P435 RT_FTL0702-1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="526-545" color="#50ff50" type="standard"/><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="1" name="P429 FTL0702_VSVG_2R" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="338-364" color="#50ff50" type="standard"/><Q name="note"><V text="P429 FTL0702_VSVG_2R"/></Q></Feature><Feature recentID="0" name="P367 FTL0702seqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="241-265" color="#8080ff" type="standard"/><Q name="note"><V text="P367 FTL0702seqF1"/></Q></Feature></Features>
  k<?xml version="1.0"?><Primers nextValidID="5"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P818 PriM_mpk_F2" sequence="atatGGATCCaattgtaaatgaatctgttactaaatattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-39" boundStrand="0" annealedBases="atatGGATCCaattgtaaatgaatctgttactaaatattc" meltingTemperature="57"><Component hybridizedRange="0-39" bases="atatGGATCCaattgtaaatgaatctgttactaaatattc"/></BindingSite><BindingSite simplified="1" location="0-39" boundStrand="0" annealedBases="atatGGATCCaattgtaaatgaatctgttactaaatattc" meltingTemperature="57"><Component hybridizedRange="0-39" bases="atatGGATCCaattgtaaatgaatctgttactaaatattc"/></BindingSite></Primer><Primer recentID="1" name="P707 PriM_unst1_R1" sequence="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="553-599" boundStrand="1" annealedBases="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" meltingTemperature="68"><Component hybridizedRange="553-599" bases="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag"/></BindingSite><BindingSite simplified="1" location="553-599" boundStrand="1" annealedBases="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" meltingTemperature="68"><Component hybridizedRange="553-599" bases="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag"/></BindingSite></Primer><Primer recentID="2" name="P821 PriM_mtip1_R3" sequence="atatGGTACCgtaactttggtattagttctgatactg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="856-882" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="856-882" bases="gtaactttggtattagttctgatactg"/></BindingSite><BindingSite simplified="1" location="856-882" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="856-882" bases="gtaactttggtattagttctgatactg"/></BindingSite></Primer><Primer recentID="3" name="P819 PriM_mpk_R2" sequence="atatGGTACCtgtaaagccaaatatactttctcttcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1315-1351" boundStrand="1" annealedBases="atatGGTACCtgtaaagccaaatatactttctcttcg" meltingTemperature="61"><Component hybridizedRange="1315-1351" bases="atatGGTACCtgtaaagccaaatatactttctcttcg"/></BindingSite><BindingSite simplified="1" location="1315-1351" boundStrand="1" annealedBases="atatGGTACCtgtaaagccaaatatactttctcttcg" meltingTemperature="61"><Component hybridizedRange="1315-1351" bases="atatGGTACCtgtaaagccaaatatactttctcttcg"/></BindingSite></Primer><Primer recentID="4" name="P708 PriM_unst1_F1" sequence="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="570-614" boundStrand="0" annealedBases="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" meltingTemperature="68"><Component hybridizedRange="570-614" bases="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg"/></BindingSite><BindingSite simplified="1" location="570-614" boundStrand="0" annealedBases="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" meltingTemperature="68"><Component hybridizedRange="570-614" bases="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg"/></BindingSite></Primer></Primers>
  e<Notes>
<UUID>5c4f04be-03e5-4bd6-8e04-af427f273645</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:32">2018.7.6</Created>
<LastModified UTC="16:1:57">2018.7.6</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  P        X        Xص:Ky$ojV!x:MfUAJff1NjAYꤩ:+MyZOz*binۺ&~J:fvj:}u_9jfꅍIۋijItkjuof       	           M  T  W  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  A<?xml version="1.0"?><Features nextValidID="15"><Feature recentID="14" name="P708 PriM_unst1_F1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="1-10" color="#50ff50" type="standard"/><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature><Feature recentID="13" name="P707 PriM_unst1_R1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="21-47" color="#50ff50" type="standard"/><Q name="note"><V text="P707 PriM_unst1_R1"/></Q></Feature><Feature recentID="12" name="P705 PriM_mtip2_F2" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="242-268" color="#50ff50" type="standard"/><Q name="note"><V text="P705 PriM_mtip2_F2"/></Q></Feature><Feature recentID="11" name="P706 PriM_mtip2_R1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="308-335" color="#50ff50" type="standard"/><Q name="note"><V text="P706 PriM_mtip2_R1"/></Q></Feature><Feature recentID="10" name="P704 CkPriM_mtip1R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="104-130" color="#50ff50" type="standard"/><Q name="note"><V text="P704 CkPriM_mtip1R"/></Q></Feature><Feature recentID="9" name="P703 CkPriM_mtip1F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="574-590" color="#50ff50" type="standard"/><Q name="note"><V text="P703 CkPriM_mtip1F"/></Q></Feature><Feature recentID="8" name="P701 PriM_mtip1_F2" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="258-283" color="#50ff50" type="standard"/><Q name="note"><V text="P701 PriM_mtip1_F2"/></Q></Feature><Feature recentID="7" name="P700 PriM_mtip1_R1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="308-335" color="#50ff50" type="standard"/><Q name="note"><V text="P700 PriM_mtip1_R1"/></Q></Feature><Feature recentID="6" name="P697 Ck_PriM_mpk1F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="52-75" color="#50ff50" type="standard"/><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature><Feature recentID="5" name="P692 PriM_mpk_R1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="14-40" color="#50ff50" type="standard"/><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="4" name="P435 RT_FTL0702-1F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="56-75" color="#50ff50" type="standard"/><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="3" name="P429 FTL0702_VSVG_2R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="237-263" color="#50ff50" type="standard"/><Q name="note"><V text="P429 FTL0702_VSVG_2R"/></Q></Feature><Feature recentID="2" name="P367 FTL0702seqF1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="336-360" color="#8080ff" type="standard"/><Q name="note"><V text="P367 FTL0702seqF1"/></Q></Feature><Feature recentID="1" name="hypothetical protein" directionality="2" translationMW="21718.23" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="27"><Segment range="1-590" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="FTL_0702" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="1-590" color="#00e600" type="standard"/></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P818 PriM_mpk_F2" sequence="atatGGATCCaattgtaaatgaatctgttactaaatattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="560-599" boundStrand="1" annealedBases="atatGGATCCaattgtaaatgaatctgttactaaatattc" meltingTemperature="57"><Component hybridizedRange="560-599" bases="atatGGATCCaattgtaaatgaatctgttactaaatattc"/></BindingSite><BindingSite simplified="1" location="560-599" boundStrand="1" annealedBases="atatGGATCCaattgtaaatgaatctgttactaaatattc" meltingTemperature="57"><Component hybridizedRange="560-599" bases="atatGGATCCaattgtaaatgaatctgttactaaatattc"/></BindingSite></Primer><Primer recentID="1" name="P707 PriM_unst1_R1" sequence="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-46" boundStrand="0" annealedBases="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" meltingTemperature="68"><Component hybridizedRange="0-46" bases="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag"/></BindingSite><BindingSite simplified="1" location="0-46" boundStrand="0" annealedBases="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" meltingTemperature="68"><Component hybridizedRange="0-46" bases="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag"/></BindingSite></Primer></Primers>
  g<Notes>
<UUID>6ee12a74-f3a8-41e0-8482-c5a809210aca</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="15:59:36">2018.7.6</Created>
<LastModified UTC="15:59:36">2018.7.6</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  L                f	=Ypz(9[d^d)N8iکd^m蕫kOy葚]I\&y_(nZ6N:ҋ>ZTfYjIHItꩨdjIzi+xiNNVDS],JxHMْ(yGd$lkX4hbQyص          
            <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="16"><Feature recentID="15" name="P708 PriM_unst1_F1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="738-762" color="#50ff50" type="standard"/><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature><Feature recentID="14" name="P707 PriM_unst1_R1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="773-782" color="#50ff50" type="standard"/><Q name="note"><V text="P707 PriM_unst1_R1"/></Q></Feature><Feature recentID="13" name="P702 PriM_mtip1_R2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="25-49" color="#50ff50" type="standard"/><Q name="note"><V text="P702 PriM_mtip1_R2"/></Q></Feature><Feature recentID="12" name="P698 Ck_PriM_mpk1R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="329-351" color="#50ff50" type="standard"/><Q name="note"><V text="P698 Ck_PriM_mpk1R"/></Q></Feature><Feature recentID="11" name="P693 PriM_mpk_F2" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="713-741" color="#50ff50" type="standard"/><Q name="note"><V text="P693 PriM_mpk_F2"/></Q></Feature><Feature recentID="10" name="P692 PriM_mpk_R1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="766-782" color="#50ff50" type="standard"/><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="9" name="P444 pKL75_F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="478-504" color="#50ff50" type="standard"/><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="8" name="P440 RT_FTL0702-3R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="525-544" color="#50ff50" type="standard"/><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="7" name="P439 RT_FTL0702-3F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="589-610" color="#50ff50" type="standard"/><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="6" name="P438 RT_FTL0702-2R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="68-89" color="#50ff50" type="standard"/><Q name="note"><V text="P438 RT_FTL0702-2R"/></Q></Feature><Feature recentID="5" name="P437 RT_FTL0702-2F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="172-194" color="#50ff50" type="standard"/><Q name="note"><V text="P437 RT_FTL0702-2F"/></Q></Feature><Feature recentID="4" name="P436 RT_FTL0702-1R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="708-729" color="#50ff50" type="standard"/><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="3" name="P411 FTL0702sp_2R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="317-341" color="#50ff50" type="standard"/><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="2" name="P368 FTL0702seqR1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="244-272" color="#8080ff" type="standard"/><Q name="note"><V text="P368 FTL0702seqR1"/></Q></Feature><Feature recentID="1" name="hypothetical protein" directionality="2" translationMW="27881.90" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="214" maxRunOn="1" maxFusedRunOn="1"><Segment range="11-782" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="FTL_0702" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="11-782" color="#00e600" type="standard"/></Feature></Features>
  2<?xml version="1.0"?><Primers nextValidID="3"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P821 PriM_mtip1_R3" sequence="atatGGTACCgtaactttggtattagttctgatactg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="469-495" boundStrand="0" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="469-495" bases="gtaactttggtattagttctgatactg"/></BindingSite><BindingSite simplified="1" location="469-495" boundStrand="0" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="469-495" bases="gtaactttggtattagttctgatactg"/></BindingSite></Primer><Primer recentID="1" name="P819 PriM_mpk_R2" sequence="atatGGTACCtgtaaagccaaatatactttctcttcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-36" boundStrand="0" annealedBases="atatGGTACCtgtaaagccaaatatactttctcttcg" meltingTemperature="61"><Component hybridizedRange="0-36" bases="atatGGTACCtgtaaagccaaatatactttctcttcg"/></BindingSite><BindingSite simplified="1" location="0-36" boundStrand="0" annealedBases="atatGGTACCtgtaaagccaaatatactttctcttcg" meltingTemperature="61"><Component hybridizedRange="0-36" bases="atatGGTACCtgtaaagccaaatatactttctcttcg"/></BindingSite></Primer><Primer recentID="2" name="P708 PriM_unst1_F1" sequence="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="737-781" boundStrand="1" annealedBases="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" meltingTemperature="68"><Component hybridizedRange="737-781" bases="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg"/></BindingSite><BindingSite simplified="1" location="737-781" boundStrand="1" annealedBases="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" meltingTemperature="68"><Component hybridizedRange="737-781" bases="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg"/></BindingSite></Primer></Primers>
  e<Notes>
<UUID>0bddf9a8-14c3-4655-9fe5-f1da1fc31f2b</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:12">2018.7.6</Created>
<LastModified UTC="16:0:12">2018.7.6</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  7zXZ  ִF !   t/Ld] ˇf{3(1v%f M*	mj)p80*i	NY]QF7&!nx_62[2B;ҿn-v_L:Ҩ-\!M%r6_u~Vke~pd*ojz%4MSxzҰJBs!1HədW UͲ?sbW&ǏY84hmʼ%Xxf>rRa=?-Rޥ`ns]Hkf?>V,g|4xIM V#go%81enwH@hL5)lYoG5~3pJc7}FV2[h!3!2RnaMao809TXZT: J3vkQk*)g2`oA_$Sc3D -πD۳IZGeM]ɮ.0y^5}?c:D|k,Wt'4XpU?|EFfY&DcP";:^ٺ!vkh}hmf2{*ٶP!gEOɈ{ut1AP mq걗r5iE(J4t?hFFt­_G8d`nۂ1p
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9u$ A˷qQDFlȊKR}:vU$SG5lgi.S Lɿ_`2ő鬪y]Z|0ʚ$9m4"֖__<rlq$˓Aail5L9tCw7bqnƝ8(Y⵿>jJ$>y$D>^^[*8^O㾑s=1?vƱ)ԝz6~pF9Ak]Co~7@{ YdLJ>~݃xh eAb 0HRl*V&#Wye9@odb,ȿu\LK6
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  Jd<?xml version="1.0"?><Features nextValidID="53"><Feature recentID="0" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="1" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="2" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="3" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="4" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="5" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="6" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="7" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7499-7518" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="8" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7477-7502" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="9" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="10" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="11" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="12" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="13" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="14" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MNIKKFAKQATVLTFTTALLAGGATQAFA,KETNQKPYKETYGISHITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK*"/></Q></Feature><Feature recentID="15" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature recentID="16" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7868-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="19" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="7514-7520" color="#ffffff" type="standard"/><Segment range="7521-7538" color="#ffffff" type="standard"/><Segment name="-35" range="7539-7544" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7559-7580" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7466-7482" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7490-7506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="P367 FTL0702seqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6338-6362" color="#8080ff" type="standard"/><Q name="note"><V text="P367 FTL0702seqF1"/></Q></Feature><Feature recentID="28" name="P429 FTL0702_VSVG_2R" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6435-6461" color="#50ff50" type="standard"/><Q name="note"><V text="P429 FTL0702_VSVG_2R"/></Q></Feature><Feature recentID="29" name="P435 RT_FTL0702-1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6623-6642" color="#50ff50" type="standard"/><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="30" name="P697 Ck_PriM_mpk1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6623-6646" color="#50ff50" type="standard"/><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature><Feature recentID="31" name="P700 PriM_mtip1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6363-6390" color="#50ff50" type="standard"/><Q name="note"><V text="P700 PriM_mtip1_R1"/></Q></Feature><Feature recentID="32" name="P701 PriM_mtip1_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6415-6440" color="#50ff50" type="standard"/><Q name="note"><V text="P701 PriM_mtip1_F2"/></Q></Feature><Feature recentID="33" name="P703 CkPriM_mtip1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6124" color="#50ff50" type="standard"/><Q name="note"><V text="P703 CkPriM_mtip1F"/></Q></Feature><Feature recentID="34" name="P704 CkPriM_mtip1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6568-6594" color="#50ff50" type="standard"/><Q name="note"><V text="P704 CkPriM_mtip1R"/></Q></Feature><Feature recentID="35" name="P706 PriM_mtip2_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6363-6390" color="#50ff50" type="standard"/><Q name="note"><V text="P706 PriM_mtip2_R1"/></Q></Feature><Feature recentID="36" name="P705 PriM_mtip2_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6430-6456" color="#50ff50" type="standard"/><Q name="note"><V text="P705 PriM_mtip2_F2"/></Q></Feature><Feature recentID="37" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-7439" color="#00e600" type="standard"/></Feature><Feature recentID="38" name="hypothetical protein" directionality="1" translationMW="48605.96" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="27" maxRunOn="7461" maxFusedRunOn="7461"><Segment range="6108-7439" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IVNESVTKYSNNVETDADTNTNSPIYAFKSIAADASNIQANGEKLARGFVLTNNGAVKIPNSIKPKNMYMKDQATAAQGLEKYRAERDEINNNIKKLESQKKLGWRIKVVAEQAKLKSINTKIDILKGIENGDKAYAEEKIAQFNIVKNITVNGPKAYFNDIAKPVLSHLNAAWDSATSLNYVDYRSNIDMFAGARVVLWNGQYNINSKDAATAIEFNDITNYLTIQTIKSLQGSDTTKAVSLYADANTLTYTVTTIGDLSSVQKKIASPRTALSICEASLISIRTNTKVTTARNIINRLSNKKAVKPILRHLLNQTSIDDIILYDLDKLGDNWALKAATNAIRSTIGSDSILYVKGHTLMLISTSSMVDAFIKAIAENEIYKQVSDADRVLFGKNACNFTAAKNSNNPIVKAMIAASKQIAGQLPKGQVIDTVFEEKVYLAL"/></Q></Feature><Feature recentID="39" name="P368 FTL0702seqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7178-7206" color="#8080ff" type="standard"/><Q name="note"><V text="P368 FTL0702seqR1"/></Q></Feature><Feature recentID="40" name="P411 FTL0702sp_2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7109-7133" color="#50ff50" type="standard"/><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="41" name="P436 RT_FTL0702-1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6721-6742" color="#50ff50" type="standard"/><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="42" name="P437 RT_FTL0702-2F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7256-7278" color="#50ff50" type="standard"/><Q name="note"><V text="P437 RT_FTL0702-2F"/></Q></Feature><Feature recentID="43" name="P438 RT_FTL0702-2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7361-7382" color="#50ff50" type="standard"/><Q name="note"><V text="P438 RT_FTL0702-2R"/></Q></Feature><Feature recentID="44" name="P439 RT_FTL0702-3F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6840-6861" color="#50ff50" type="standard"/><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="45" name="P440 RT_FTL0702-3R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6906-6925" color="#50ff50" type="standard"/><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="46" name="P444 pKL75_F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6946-6972" color="#50ff50" type="standard"/><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="47" name="P692 PriM_mpk_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6658-6684" color="#50ff50" type="standard"/><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="48" name="P693 PriM_mpk_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6709-6737" color="#50ff50" type="standard"/><Q name="note"><V text="P693 PriM_mpk_F2"/></Q></Feature><Feature recentID="49" name="P698 Ck_PriM_mpk1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7099-7121" color="#50ff50" type="standard"/><Q name="note"><V text="P698 Ck_PriM_mpk1R"/></Q></Feature><Feature recentID="50" name="P702 PriM_mtip1_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7401-7425" color="#50ff50" type="standard"/><Q name="note"><V text="P702 PriM_mtip1_R2"/></Q></Feature><Feature recentID="51" name="P707 PriM_unst1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6651-6677" color="#50ff50" type="standard"/><Q name="note"><V text="P707 PriM_unst1_R1"/></Q></Feature><Feature recentID="52" name="P708 PriM_unst1_F1" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6688-6712" color="#50ff50" type="standard"/><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature></Features>
  o<?xml version="1.0"?><Primers nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7493-7512" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="7493-7512" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7493-7512" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="7493-7512" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="1" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="2" name="P708 PriM_unst1_F1" sequence="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6667-6711" boundStrand="0" annealedBases="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" meltingTemperature="68"><Component hybridizedRange="6667-6711" bases="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg"/></BindingSite><BindingSite simplified="1" location="6667-6711" boundStrand="0" annealedBases="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" meltingTemperature="68"><Component hybridizedRange="6667-6711" bases="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg"/></BindingSite></Primer><Primer recentID="3" name="P819 PriM_mpk_R2" sequence="atatGGTACCtgtaaagccaaatatactttctcttcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7412-7444" boundStrand="1" annealedBases="GGTACCtgtaaagccaaatatactttctcttcg" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="7412-7444" bases="GGTACCtgtaaagccaaatatactttctcttcg"/></BindingSite><BindingSite simplified="1" location="7412-7444" boundStrand="1" annealedBases="GGTACCtgtaaagccaaatatactttctcttcg" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="7412-7444" bases="GGTACCtgtaaagccaaatatactttctcttcg"/></BindingSite></Primer><Primer recentID="4" name="P821 PriM_mtip1_R3" sequence="atatGGTACCgtaactttggtattagttctgatactg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6953-6979" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6953-6979" bases="gtaactttggtattagttctgatactg"/></BindingSite><BindingSite simplified="1" location="6953-6979" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6953-6979" bases="gtaactttggtattagttctgatactg"/></BindingSite></Primer><Primer recentID="5" name="P707 PriM_unst1_R1" sequence="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6650-6696" boundStrand="1" annealedBases="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" meltingTemperature="68"><Component hybridizedRange="6650-6696" bases="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag"/></BindingSite><BindingSite simplified="1" location="6650-6696" boundStrand="1" annealedBases="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" meltingTemperature="68"><Component hybridizedRange="6650-6696" bases="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag"/></BindingSite></Primer><Primer recentID="6" name="P818 PriM_mpk_F2" sequence="atatGGATCCaattgtaaatgaatctgttactaaatattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6101-6136" boundStrand="0" annealedBases="GGATCCaattgtaaatgaatctgttactaaatattc" meltingTemperature="57"><Component bases="atat"/><Component hybridizedRange="6101-6136" bases="GGATCCaattgtaaatgaatctgttactaaatattc"/></BindingSite><BindingSite simplified="1" location="6101-6136" boundStrand="0" annealedBases="GGATCCaattgtaaatgaatctgttactaaatattc" meltingTemperature="57"><Component bases="atat"/><Component hybridizedRange="6101-6136" bases="GGATCCaattgtaaatgaatctgttactaaatattc"/></BindingSite></Primer></Primers>
  f<Notes>
<UUID>ca36b82e-ba13-41ab-877b-9ffd60beb521</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:2:6">2018.7.6</Created>
<LastModified UTC="16:17:31">2019.1.21</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         Unique 6+ Cutters                                                                                            d     CHO  d              E              Y? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
