	   SnapGene      TATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAATCGATCAAGGGTTGGTTTGCGCATTCACAGTTCTCCGCAAGAATTGATTGGCTCCAATTCTTGGAGTGGTGAATCCGTTAGCGAGGTGCCGCCGGCTTCCATTCAGGTCGAGGTGGCCCGGCTCCATGCACCGCGACGCAACGCGGGGAGGCAGACAAGGTATAGGGCGGCGCCTACAATCCATGCCAACCCGTTCCATGTGCTCGCCGAGGCGGCATAAATCGCCGTGACGATCAGCGGTCCAATGATCGAAGTTAGGCTGGTAAGAGCCGCGAGCGATCCTTGAAGCTGTCCCTGATGGTCGTCATCTACCTGCCTGGACAGCATGGCCTGCAACGCGGGCATCCCGATGCCGCCGGAAGCGAGAAGAATCATAATGGGGAAGGCCATCCAGCCTCGCGTCGATCTATACCCTTCAAGCTTTGAAAAATAAACTTAATTATTATATATGTTATTTAGCTAGTTTTTTTAATTAAAGTTAAAATCGAGAGCTTGTTTGACAAAAAAACAAAAAAATTTCTTGAAAATTTTTTTTTTGACTCAATATCTAGACTTGCAAGAGCTTGGAACTTTGAGATTGTTCTAAGATGCATACAAATTCAAAATGCTTAAACAAAAATAATTTAACAAAGGAGTAAGATTGTTATGAGCCATATTCAACGGGAAACGTCTTGCTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGATCACGCATCTTCCCGACAACGCAGACCGTTCCGTGGCAAAGCAAAAGTTCAAAATCACCAACTGGTCCACCTACAACAAAGCTCTCATCAACGATATCCCGCAAGAGGCCCGGCAGTACCGGCATAACCAAGCCTATGCCTACAGCATCCAGGGTGACGGTGCCGAGGATGACGATGAGCGCATTGTTAGATTTCATACACGGTGCCTGACTGCGTTAGCAATTTAACTGTGATAAACTACCGCATTAAAGCTAATCGGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAGAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATAATCGGCATTTTCTTTTGCGTTTTTATTTGTTAACTGTTAATTGTCCTTGTTCAAGGATGCTGTCTTTGACAACAGATGTTTTCTTGCCTTTGATGTTCAGCAGGAAGCTAGGCGCAAACGTTGATTGTTTGTCTGCGTAGAATCCTCTGTTTGTCATATAGCTTGTAATCACGACATTGTTTCCTTTCGCTTGAGGTACAGCGAAGTGTGAGTAAGTAAAGGTTACATCGTTAGGATCAAGATCCATTTTTAACACAAGGCCAGTTTTGTTCAGCGGCTTGTATGGGCCAGTTAAAGAATTAGAAACATAACCAAGCATGTAAATATCGTTAGACGTAATGCCGTCAATCGTCATTTTTGATCCGCGGGAGTCAGTGAACAGATACCATTTGCCGTTCATTTTAAAGACGTTCGCGCGTTCAATTTCATCTGTTACTGTGTTAGATGCAATCAGCGGTTTCATCACTTTTTTCAGTGTGTAATCATCGTTTAGCTCAATCATACCGAGAGCGCCGTTTGCTAACTCAGCCGTGCGTTTTTTATCGCTTTGCAGAAGTTTTTGACTTTCTTGACGGAAGAATGATGTGCTTTTGCCATAGTATGCTTTGTTAAATAAAGATTCTTCGCCTTGGTAGCCATCTTCAGTTCCAGTGTTTGCTTCAAATACTAAGTATTTGTGGCCTTTATCTTCTACGTAGTGAGGATCTCTCAGCGTATGGTTGTCGCCTGAGCTGTAGTTGCCTTCATCGATGAACTGCTGTACATTTTGATACGTTTTTCCGTCACCGTCAAAGATTGATTTATAATCCTCTACACCGTTGATGTTCAAAGAGCTGTCTGATGCTGATACGTTAACTTGTGCAGTTGTCAGTGTTTGTTTGCCGTAATGTTTACCGGAGAAATCAGTGTAGAATAAACGGATTTTTCCGTCAGATGTAAATGTGGCTGAACCTGACCATTCTTGTGTTTGGTCTTTTAGGATAGAATCATTTGCATCGAATTTGTCGCTGTCTTTAAAGACGCGGCCAGCGTTTTTCCAGCTGTCAATAGAAGTTTCGCCGACTTTTTGATAGAACATGTAAATCGATGTGTCATCCGCATTTTTAGGATCTCCGGCTAATGCAAAGACGATGTGGTAGCCGTGATAGTTTGCGACAGTGCCGTCAGCGTTTTGTAATGGCCAGCTGTCCCAAACGTCCAGGCCTTTTGCAGAAGAGATATTTTTAATTGTGGACGAATCGAACTCAGGAACTTGATATTTTTCATTTTTTTGCTGTTCAGGGATTTGCAGCATATCATGGCGTGTAATATGGGAAATGCCGTATGTTTCCTTATATGGCTTTTGGTTCGTTTCTTTCGCAAACGCTTGAGTTGCGCCTCCTGCCAGCAGTGCGGTAGTAAAGGTTAATACTGTTGCTTGTTTTGCAAACTTTTTGATGTTCATCGTTCATGTCTCCTTTTTTATGTACTGTGTTAGCGGTCTGCTTCTTCCAGCCCTCCTGTTTGAAGATGGCAAGTTAGTTACGCACAATAAAAAAAGACCTAAAATATGTAAGGGGTGACGCCAAAGTATACACTTTGCCCTTTACACATTTTAGGTCTTGCCTGCTTTATCAGTAACAAACCCGCGCGATTTACTTTTCGACCTCATTCTATTAGACTCTCGTTTGGATTGCAACTGGTCTATTTTCCTCTTTTGTTTGATAGAAAATCATAAAAGGATTTGCAGACTACGGGCCTAAAGAACTAAAAAATCTATCTGTTTCTTTTCATTCTCTGTATTTTTTATAGTTTCTGTTGCATGGGCATAAAGTTGCCTTTTTAATCACAATTCAGAAAATATCATAATATCTCATTTCACTAAATAATAGTGAACGGCAGGTATATGTGATGGGTTAAAAAGGATCGATCCTCTAGCTAGAGTCGATCTTCGCCAGCAGGGCGAGGATCGTGGCATCACCGAACCGCGCCGTGCGCGGGTCGTCGGTGAGCCAGAGTTTCAGCAGGCCGCCCAGGCGGCCCAGGTCGCCATTGATGCGGGCCAGCTCGCGGACGTGCTCATAGTCCACGACGCCCGTGATTTTGTAGCCCTGGCCGACGGCCAGCAGGTAGGCCGACAGGCTCATGCCGGCTGCCGCCGCCTTTTCCTCAATCGCTCTTCGTTCGTCTGGAAGGCAGTACACCTTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGCTGCTTCCCTGCTGTTTTGTGGAATATCTACCGACTGGAAACAGGCAAATGCAGGAAATTACTGAACTGAGGGGACAGGCGAGAGACGATGCCAAAGAGCTACACCGACGAGCTGGCCGAGTGGGTTGAATCCCGCGCGGCCAAGAAGCGCCGGCGTGATGAGGCTGCGGTTGCGTTCCTGGCGGTGAGGGCGGATGTCGATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCATATTTGAATGTATTTAGAAAAATAAACAAAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACAGCCAAGCTCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCaattgtaaatgaatctgttactaaatattctaataatgttgagacagatgctgatactaatacaaattcaccaatttatgcattcaaaagcatagcagctgatgcaagtaatattcaagcaaatggagaaaaattagctagaggttttgtcttaactaacaatggtgctgttaagataccgaactctatcaagccaaaaaatatgtatatgaaagatcaagcaactgctgctcaaggtcttgagaaatacagagctgaaagagatgagattaataacaacatcaaaaaacttgaaagtcaaaagaaattaggctggagaattaaagtagttgctgaacaagctaaactaaaaagtattaacactaaaatagatatcctaaaaggtatcgaaaatggtgataaagcttatgctgaagagaaaatcgctcagtttaatatcgtaaaaaatataactgtaaatggtcctaaggcgtattttaatgatattgctaagccagtattatcacatttaaatgcagcatgggattctgctactagtcttaactatgtagactacagaagcaacattgatatgttcGCAggtgcaGCAgtagttcttGCAaatggtcaatataacatcaatagcaaagatgccgctactgccattgaatttaacgatatcactaactacttgactattcagactattaagtctcttcaaggtagtgatacaactaaagcagtttcactaGCAgctgatgctaacacacttacatatacagtaactactataggcgacttaagtagtgttcagaaaaaaatagcttcgccaagaacggctctatcaatttgtgaagctagcttaatcagtatcagaactaataccaaagttactacagctagaaatattattaacagactatcaaataaaaaagctgtaaaacctatactaagacacttactaaatcaaactagtattgatgatattatactttacgacctagataaattaggcgacaactgggcacttaaagcagctacaaatgcaattagatcaacaataggttctgatagtatactatacgttaagggtcatactttaatgttaatatctacaagtagtatggttgatgcctttatcaaggctattgctgagaatgaaatttacaagcaagtaagtgatgctgatagagttctatttggtaaaaatgcatgtaattttacagcagctaaaaatagtaacaatcctatagttaaagctatgattgcagcttctaaacagatagccggacaacttcctaaaggtcaagttatcgacaccgttttcgaagagaaagtatatttggctttacaGGTACCGAGCTCGAATTCGTAATCATGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACCCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAAC  E                     "   
                &         #               "   3         4   &   "                                 )   %      &   '   ;   %               '         !   /   %   &      5      %   $   '   0   #                (      /   "            *         )   /      &   +       (   =   h   G   &   $   5   *   0      (   '      #      #   -      (   .   "   !   $   <      #   4      "               !   '            "                           "   #      (   :         %   8      "   %   %   -                  D   /   "          +   *                   9   /   .      6   %      E   /   h   3      0   %   $            "   1   *   '   !         &            $               "                     -            %   $   +   '   #   /   %   3               '                     '   (   $      #         $      0   !      $                  '   #            #                              7ZDU	C
afg|k \[E_
yb4hli 8	V	2
<
22} n&
D
?o
9F \A	
0cW  H/F`2f
X>[k|5fw"j" @ ]x.	

b6Kz0dimO
 H{  Z p  9		3
=
:zA~e~  9 O    h@;KYw=R  j).	W	U@F!3i+Z 9d3'	B?	3`7(& W or	'
9

WHBw:_v 6Or ,  _
W`V
|
`rL ~ 'A+P/E
I
(%=0# s <JqTR	{x)@NZpR|"G M 7]oP	94g j5E		k
r71dXzG 	 c  	B7sm
JCgl I QG*	
]
tQ=_o 3a9" k    07G	
C4Z,6wg#k3  Mragk*G	k			#?l}3XpbZ
_|LM=x :3"T:	P^L\MaIe  I \	h,QYH")1|4 &`c2Aip	



4e]j	'Ej	?O/b& FOD/	yL9_MhjhnP" A ^l	Hw;b

{1ey  #+y]zci7= >P/Dy			sI|  c			

%   "9g/{[ D2|&f? n q  F:&FYp 9		H2;f`; 4
>
F.SU<79q [ p	o
,{Bm . T   /VLm ($)  : DK
LPW ,Fo\x!sS% P   iA>		BkQ<Z+'> X[f
<p2&  /Y6

GY}l1 #,'  WLk	

V^(09ei@4?C[;   *`u	


+A`{~>	VZwE"Ti<j2% e X	

tP+n,: 4Z@
Jd47a) e	"
,
8	)  :f	b#	|		


Cj$@
(o  (
$W' X  Xp,		
0
:
T
>v=0XmyI<D4;P K N3ux5A} ;`w7 X I		*


@}*n!  
s]		(

v'.DCx)"0s/ - 0 F  `]X	
_

16	K\a/9yM9"%t%   SL
7	asu+rBQ1JX& Mfpax	W
}
Y<E]*4j I_	q
nq"5   ,QF

Sm)gqFQ&\o<&tlDe/1 ]  
zPc/UH>% (m(0	J
5	 D2In"Hu$   ABkl8	euJ$D&2' !$),r
 x )OEORcE0 & 3  KS|r	|y?dq+Sv}X[# t uS;Ukq
 M\*AW5HH2x[ =S	
NR[6# [m~	T	%
x18ej ? w@

a5JW/c{/v  N 28g:T_yhX( V ^IpQ		
z(MVqA{!$u<N H b  OD	\
l
s6B^ffyk/   69j)F		O3"G2WoY	{0KHd{" "kGa

h38\&LN  b!	
r{8e Z~ m E
8o	n
ER1_p  CZKOE;'*#&[* d   _tXdhvDhO~"*>m 
 a' J  2 H+	
/
@=u?|<;C:m) / E   Lw	
^

J`p.38P
$IWi/  l7		
dRt[;b.m!Gk.C% 2  RRj
M>*NV4bw& ZvfH?
Sy..z 7dtW;M+    !8FiN	gq5[zFexM}z  l `n	DNc}D:="Z^o(   1kK6i	


-B2O-l	jVhv$l=  u>h5m	Rpy-EAdyc1 9VkL<inuLyh   Nstst#0189l				uvwx$,MNO@4YZcwx0[\78GU`}fghqz{G u$2{l:XH	P		"%Pu~5[S]Ha~3MbN|>&   Oy,b&t+L			
 m_q2r$ A%	Mm;	##vNT{b4J}5 v3#;U	I	Q		r]6\HI;F6NcU* 6|4Y
_B#^bO?P0 {;I}x
9YiQM&Q&#f JU\c&,A
!rgq( <   -;]2M	
tn`	x*A@WZ}' x Pz'u
#N

iRZI-t3s2   e			-#s# BhN>D^
_U|c5) hO
a BRq(			f$K# R <	

$"0	Ok'- G'&9d3njo	
R

5k7w(F
(@rP0c~ kR		M t6JGi,}( ?w4$rGVcJ		v
	s*!]i>dlo	. V<PE	4	:`^GIN<kO " b0	mz77x]VQ! 
ICZ	 	c


(C g^A@$ B mx$				Sz_>QT<  _75O<e	6	@	c	

ntU`<bi|2mISA$cPs .X}6df ,KQ#  # y
[]b[Nt>\4 p<H~>			Y
 d:FZjR''>Cm'-_8$@g  ,z^$&8RV<`"  $|!NT{JGzj. PF^ | 5]0'-		=}	X  KEB	#Jh_ Udz	
-|"}  ?QVtlCsVr! = `  
'u	
>o$
EXb'  dy.<^03N	

wpay+ TNX 2	a			&YrA[~   	ulBV ) 6 (u$h

+j4h0.X" y  #O
:Jnu4Dpt|3B\z  {vI[w QZnSWH  K ]o$sd9L  Er f	s
3q 9   ,u			.}tS  d'g@ C	`3{ GeGZjV!" 8 o     ci-?&EA }d6*v# + r ;*OS	8
H
'_K^q  iPr)	j
8I3P<n 2F( j  q![S	9		g+YL(v`L<:  *_b1:@ENh	

x^iD>g.ag =.Cx				
1%z G-T%;E6Xl8:% S =	
e
VUo1jPlnr8  KX5






*'/=s8UY|?S01B9 "	 
?



Ici#
n(V"  w4S


-l|)(C!3/%  Heo\	p
6X058xq)A!0% \  'jk
~lpCHPn%sCd-  (:{pq	

[5GQZ1D U lS		
`I  C	2N!VneKJj 0Wgu!)% D   c;M7aH -~  YE	
M-||]D x+z%sWK		Q
!ot+DL^16FTjepymt
/ @~5LH|w

XA"G%5"T" ; w :%d	M

ssw@?frVp  Q N8=QnQ
C/6'q( ja	s =w+  WB	
		5	?		
;alHR@RJ=~PS@r*   "  oSF			%U_O[l,^7E?] { 4,{k<B|U^W  _  x&/t1	=XqDS Ymg
*3VWvAy  c\nc8p8KR   b,7
@2u9  i  )p0?ZDg	8	


7w]XhmKC_y-/  J!		d





)H:bDWkmA.  iz[

}ZW/o~4~4$/B C B	_1bH:fU`6 X}*9y	L


r
n@{r?e$U% ! nM7R						
$.r5?&pR6D\  l%W)jAT@R
JE    (ao+>yf7	




|v?Va.n}W3VJjBlt/ 86Q	A	
q	a#Gm?-qT>%d5Q	h     /`P=x23vw 	




opKLMuF
-=>cdjklm*+,_nopU=k3 04ax	d			

,1 BNe[`xF#+83^w  		2,9M-f0 }/h Y!5b	

!CO_fuaG$!-L% f 1y

)7F\ey,o_x$ *~@Y	e	-IgKQ4R;  	A
z
8u?&.g 5[mnc\O-]  	\^5:*," $ V	3KebwZ[N 1 C0	#
-
Z

;P=UvH(?"(.Ah* qi!ZI6?	
eg.M9D`9J' f="c<&		"D[k`Sg (b%:
%*{! S~p		!GnLF   ;c	


%AWhz= 2_z}	*Sap&  g  -{g>


'8DkG] )Kp- 
:$		9;%fY4`y  AUZ	/G<X  +D}O){k%JS_$ %\K
.'RQ56 "|Hf		hLR(b 6Y^
1
;
B
>1nEh  .1-		

z=eN  Y  (			?>9J@'<QY" }  &@q.V_U
{
wYGQv~
/5 L 64n6F	q`8 Lj	B]KP!~+a^ %\vyoCd<xi  OC$f
.> +~-o WJ	 .A+ - V	

]_z}~k;+  Ye{		!
+
#6 	MWWt\			)
u&/x RK^	
m[!;Dt\*s%   @4f1EyOTI
#1Gt$ % #t+wL(QcDuWZ+ > 1}	$

'Vw
LU^bx0pz@#iu' G a a (
[
`

27A]%/Yc#   D^Y~Q &N&)/ . 1  Qv		.

?<>{LIs)U:Z:#FB%  ejJ_Mh

fxbsCN,!:UY3   r"/=74O	

tbv/R2K;aK  z	8hq"z:EO  T["1@
,Y gFg'			!^& >Uqb3	u
l)F5+\k|' -N~#d	b			~
'ms5Thcf+]  MG =yX
Z#E\aZ&-;=sB  T	" `			vo#kMUWa ( 5  tHmCoG  Jq+t	r
r * 7  )Rd%G
iJ]z '  <-^Mw		i
O*01='uEf( W


&,Tnhr5TXi{>R/m078U$   v
kBGO'pY 2C  
dI# v ${~	CKv05ESq4{ `m	P
TQ}]v?q ^ z  3w.s+;bUo{VuC$  h  h	

69\ 3.x   |)K
qo#I0 '.nw1	


0J"El,)^*74]v!  |)g? 
(^EHJLe Ulm	vYZ%+$ BRo;%	f<T9KEIz   =9		:gXJ3 C[	&Q'5a   %?-U^	
FPdM $Bi	p *e	= V 
y*j'  "L*sJ[	%P0t :SH	FY#  P|} z&K@T]rTw%Y  !g!: ,E~LTF}	t
l4e,~r\ ' 4  s*s	jT`# ]VLv	N/SNI6y7BT u v-lr	~BTRu  &f	

/!]+(63\d T<	P8XI*  >#~ITh		
i) S\$i e	O7MS)Ac }g	(h  0  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                       3                                                      
      	                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       	                                                                                                                        	                                                                                                                                         +               	                                             	                                                                  	                              
   	                     	      	                        ,               	                                                	                     
                                       '   '      /   !         #            s      %   _   `   *         6   n                                                                                                                                                                            3                          
  	                                                                                                                                                                                                                                                                   	                                              	                                                       +     	                 	                        	          
 	       	  	        ,     	                	       
             ' '  / !   #    s  % _ ` *   6 n         .      b
u I	*r)*F"/1 #q
^OR4cccc9XvS 'OR[dS<;9&Rx;B

g
 5I&Zc eDkDN	 y	(y	( r~H*	9
wQ{3^aN ury	C~|o
oo
lo
b	+r.	O$^ sTCruCw rArW	~ 6<d,}|S\y*L$]EO <'		o
oof&		~=J4w $QF
yKmqu2EOSDX\7
	~,"xh6t	mXDMj u.  
I# kOdn(~
kl(o

F g  f"\cK&			!eUg A<$EO .
<:Z:lZ: l6	HN[&e
o1dixb6 #zii hp YZ5		8	
XK-L_|&T]wIy	#		$	d- 0  	9F&o9"1<'R	?Zo"F >~T	
		:e~p'- 0  	99"F&o1LF,~r]	| -}	 x )- 0  !p)r	T
vOX9F&o9"R14E >~T|	
&T]wIyxW
Y<&c2i

4j'E	?O/b) 6 ($h } %

i M y-B<Ti
O1E ' 		
+4.XB~	i
<OT1{EDQ ( 5 t>PsX*
IX0
:
0mD	 :PLeeOY6 ;A	Z
.< f]dG6B	&
:  k	V(i@?  74Zw{>'G K	 	:PLee!p)rT
vOXR4E( 5 DtQ  RfK!b!C_fa$0U	MWWt\			)
u&/x "L*sJ[	%P0t :SH	FYX  Xp,		
0
:
T
>v=0XmyI<D4;PX[f
<p2 l7		
dRt[;b.m!Gk.C% 
s]		(

v'.DCx)"0s 
dI #+y]zci7=s <JqTR	{x)@NZpR|"G  %?-U^	
FPdMgFg'			!^&2 s ~   '<AJRlRq+PT/7Rj			
E
I

{dR(x)M@>NZpt*NRV|"4%G[;b.m!Gk=.bw0C%Q gFj>aNU8gq=Qbn'	3				Q
u
lC!/)s 6F^'5q=w(&+\k|Udz	
-|"}  Heo\	p
6X058xq)A!0   %?-P]VLU^|v		
} N/z&KS@T]rFPTwN%IY6dy7BMT * 7 >   %1?-)Rd%U^}		
$
G

'Vw
LU^FPbx0ipz@J#]dziu M6|4Y
_B#^bO?PT<	P8XI*M 7]oP	94gX}*9y	L


r
n@{r?e$UN   U28g ld,7u	z					

:Td-R|."}_t}y[;bh.Xm!Gkt .C%S5[mnc\O-]  n       !        !Л.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAQb^MjZ RXW9RSI[nZ)jjnZ]kmiiYojm]JfffUVVUZifjVWVYjUԵuVQ^luJᆥcUh(GF8guk}jckU6M8cRYMXUB^]`)F|^UF׎;PY8TF]UTQZC$yU&yVFoBlUS8Ql*YUYX/2jmڕ69^bGhf+4#M$X-f]TcxqyyyiK$uM*M8cwftd1I"UVN\;ZSET9ityeU8~gwy&z~FV^zTӝX֑Bdrgbnu$(cmC8Eju5%!%V6%tӕY%9:tduBԦ|Rft'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ                      !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  X<?xml version="1.0"?><Features recycledIDs="7,18,10,11,13,14,15,16,17,3,55,50,48" nextValidID="76"><Feature recentID="75" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7978-7994" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="74" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7954-7970" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="73" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="72" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8047-8068" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="71" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="70" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8002-8008" color="#ffffff" type="standard"/><Segment range="8009-8026" color="#ffffff" type="standard"/><Segment name="-35" range="8027-8032" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="69" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="68" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="67" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="66" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="65" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8356-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="64" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="63" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="62" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="61" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="60" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="59" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="58" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="57" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7965-7990" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="56" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7987-8006" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="54" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="53" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="52" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="51" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="49" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="47" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="46" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="45" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6524-7927" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3951071" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0702"/></Q></Feature><Feature recentID="44" name="FTL_0702" directionality="1" translationMW="51048.96" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7949" maxFusedRunOn="7949"><Segment range="6519-7927" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="YP_513453.1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="inference"><V predef="ab initio prediction"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="db_xref"><V text="89256091" predef="GI"/><V text="3951071" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0702"/></Q></Feature><Feature recentID="43" name="P364 FTL0702cmp_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6524-6550" color="#a020f0" type="standard"/><Q name="note"><V text="P364 FTL0702cmp_F"/></Q></Feature><Feature recentID="42" name="glyA" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6246" color="#a6acb3" type="standard"/><Q name="gene"><V text="glyA"/></Q><Q name="db_xref"><V text="3951072" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0703"/></Q></Feature><Feature recentID="41" name="glyA" directionality="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6265" maxFusedRunOn="6265"><Segment range="6113-6246" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="EC_number"><V text="2.1.2.1"/></Q><Q name="protein_id"><V text="YP_513454.1"/></Q><Q name="product"><V text="serine hydroxymethyltransferase"/></Q><Q name="gene"><V text="glyA"/></Q><Q name="inference"><V bool="1" text="2004" predef="profile"/></Q><Q name="note"><V text="catalyzes the reaction of glycine with 5,10-methylenetetrahydrofolate to form L-serine and tetrahydrofolate"/><V text="/dnas_title=serine hydroxymethyltransferase"/></Q><Q name="db_xref"><V text="89256092" predef="GI"/><V text="3951072" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0703"/></Q></Feature><Feature recentID="40" name="RPp4" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6506-6532" color="#a6acb3" type="standard"/><Q name="note"><V text="RPp4"/></Q></Feature><Feature recentID="39" name="PRP12" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6345-6374" color="#a020f0" type="standard"/><Q name="note"><V text="PRP12"/></Q></Feature><Feature recentID="38" name="200 bp max AT content (91%)" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6306-6505" color="#a6acb3" type="standard"/><Q name="note"><V text="200 bp max AT content (91%)"/></Q></Feature><Feature recentID="37" name="promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6474-6474" color="#ffffff" type="standard"/><Q name="note"><V text="Potential TSS"/></Q></Feature><Feature recentID="36" name="Potential -10 element" type="-10_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6462-6467" color="#a6acb3" type="standard"/><Q name="note"><V text="Potential -10 element"/></Q></Feature><Feature recentID="35" name="Potential -35 element" type="-35_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6440-6445" color="#a6acb3" type="standard"/><Q name="note"><V text="Potential -35 element"/></Q></Feature><Feature recentID="34" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6345-6374" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P431 P_FTL0702_F"/></Q></Feature><Feature recentID="33" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6473-6506" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P434 FTL0702cmp_F2"/></Q></Feature><Feature recentID="32" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6216-6237" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P478 RT_up0702-1_F"/></Q></Feature><Feature recentID="31" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6223-6249" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P480 RT_up0702-2_F"/></Q></Feature><Feature recentID="30" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6151-6175" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P482 RT_up0702-3_F"/></Q></Feature><Feature recentID="29" name="P691 PriM_mpk_F1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6134" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P691 PriM_mpk_F1"/></Q></Feature><Feature recentID="28" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-7932" color="#ffffff" type="standard"/><Q name="mol_type"><V predef="genomic DNA"/></Q><Q name="strain"><V text="LVS"/></Q><Q name="note"><V text="/dnas_title=pKL115 pEX_PriM_mpk1B"/></Q><Q name="db_xref"><V text="119857" predef="taxon"/></Q><Q name="sub_species"><V text="holarctica"/></Q><Q name="organism"><V text="Francisella tularensis subsp. holarctica"/></Q></Feature><Feature recentID="27" name="P367 FTL0702seqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6831-6855" color="#a020f0" type="standard"/><Q name="note"><V text="P367 FTL0702seqF1"/></Q></Feature><Feature recentID="26" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6928-6954" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P429 FTL0702_VSVG_2R"/></Q></Feature><Feature recentID="25" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7602-7626" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="24" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7116-7135" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="23" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7214-7235" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="22" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7331-7354" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="21" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7399-7418" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="20" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7439-7465" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="19" name="FTL_0701" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7928-7932" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3951070" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0701"/></Q></Feature><Feature recentID="12" name="P368 FTL0702seqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7671-7699" color="#a020f0" type="standard"/><Q name="note"><V text="P368 FTL0702seqR1"/></Q></Feature><Feature recentID="9" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7749-7771" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P437 RT_FTL0702-2F"/></Q></Feature><Feature recentID="8" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7854-7875" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P438 RT_FTL0702-2R"/></Q></Feature><Feature recentID="6" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7151-7201" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="5" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7178-7230" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P693 PriM_mpk_F2"/></Q></Feature><Feature recentID="4" name="Altered region!" type="misc_difference" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7173-7328" color="#ff0000" type="standard"/><Q name="note"><V text="Altered region!"/></Q></Feature><Feature recentID="2" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7324-7359" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P696 PriM_mpk_F4"/></Q></Feature><Feature recentID="1" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7592-7614" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P698 Ck_PriM_mpk1R"/></Q></Feature><Feature recentID="0" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7116-7139" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature></Features>
  
<?xml version="1.0"?><Primers nextValidID="4"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="3" name="P819 PriM_mpk_R2" sequence="atatGGTACCtgtaaagccaaatatactttctcttcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7900-7932" boundStrand="1" annealedBases="GGTACCtgtaaagccaaatatactttctcttcg" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="7900-7932" bases="GGTACCtgtaaagccaaatatactttctcttcg"/></BindingSite><BindingSite simplified="1" location="7900-7932" boundStrand="1" annealedBases="GGTACCtgtaaagccaaatatactttctcttcg" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="7900-7932" bases="GGTACCtgtaaagccaaatatactttctcttcg"/></BindingSite></Primer><Primer recentID="2" name="P696 PriM_mpk_F4" sequence="ttcactaGCAgctgatgctaacacacttacatatac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7318-7353" boundStrand="0" annealedBases="ttcactaGCAgctgatgctaacacacttacatatac" meltingTemperature="62"><Component hybridizedRange="7318-7353" bases="ttcactaGCAgctgatgctaacacacttacatatac"/></BindingSite><BindingSite simplified="1" location="7318-7353" boundStrand="0" annealedBases="ttcactaGCAgctgatgctaacacacttacatatac" meltingTemperature="62"><Component hybridizedRange="7318-7353" bases="ttcactaGCAgctgatgctaacacacttacatatac"/></BindingSite></Primer><Primer recentID="1" name="P695 PriM_mpk_R3" sequence="catcagcTGCtagtgaaactgctttagttgtatcact" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7298-7334" boundStrand="1" annealedBases="catcagcTGCtagtgaaactgctttagttgtatcact" meltingTemperature="64"><Component hybridizedRange="7298-7334" bases="catcagcTGCtagtgaaactgctttagttgtatcact"/></BindingSite><BindingSite simplified="1" location="7298-7334" boundStrand="1" annealedBases="catcagcTGCtagtgaaactgctttagttgtatcact" meltingTemperature="64"><Component hybridizedRange="7298-7334" bases="catcagcTGCtagtgaaactgctttagttgtatcact"/></BindingSite></Primer><Primer recentID="0" name="P818 PriM_mpk_F2" sequence="atatGGATCCaattgtaaatgaatctgttactaaatattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6595-6624" boundStrand="0" annealedBases="aattgtaaatgaatctgttactaaatattc" meltingTemperature="52"><Component bases="atatGGATCC"/><Component hybridizedRange="6595-6624" bases="aattgtaaatgaatctgttactaaatattc"/></BindingSite><BindingSite simplified="1" location="6595-6624" boundStrand="0" annealedBases="aattgtaaatgaatctgttactaaatattc" meltingTemperature="52"><Component bases="atatGGATCC"/><Component hybridizedRange="6595-6624" bases="aattgtaaatgaatctgttactaaatattc"/></BindingSite></Primer></Primers>
  A<Notes>
<UUID>c612d86d-1ad4-4bc9-9393-f55b37023277</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:22:37">2018.7.5</Created>
<LastModified UTC="23:59:52">2018.7.5</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  {'        #        #.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAQb^MjZ RXW9RSI[nZ)jjnZ]kmiiYojm]JfffUVVUZifjVWVYjUԵuVQ^luJᆥcUh(GF8guk}jckU6M8cRYMXUB^]`)F|^UF׎;PY8TF]UTQZC$yU&yVFoBlUS8Ql*YUYX/2jmڕ69^bGhf+4#M$X-f]TcxqyyyiK$uM*M8cwftd1I"UVN\;ZSET9ityeU8~gwy&z~FV^zTӝX֑Bdrgbnu$(cmC8Eju5%!%V6%tӕY%9:tduBԦ|Rfu*zRX>TQayajZZNެwcYueYj٨IjDgJIm:ڍM9حk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>                     )  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  d<?xml version="1.0"?><Features recycledIDs="3,55,50,48" nextValidID="76"><Feature recentID="75" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8285-8301" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="74" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8261-8277" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="73" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="72" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8354-8375" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="71" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="70" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8309-8315" color="#ffffff" type="standard"/><Segment range="8316-8333" color="#ffffff" type="standard"/><Segment name="-35" range="8334-8339" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="69" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="68" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="67" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="66" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="65" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8663-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="64" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="63" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="62" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="61" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="60" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="59" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="58" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="57" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8272-8297" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="56" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8294-8313" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="54" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="53" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="52" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="51" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="49" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="47" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="46" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="45" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6524-7981" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3951071" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0702"/></Q></Feature><Feature recentID="44" name="FTL_0702" directionality="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7988" maxFusedRunOn="7988"><Segment range="6519-7981" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="YP_513453.1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="inference"><V predef="ab initio prediction"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="db_xref"><V text="89256091" predef="GI"/><V text="3951071" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0702"/></Q></Feature><Feature recentID="43" name="P364 FTL0702cmp_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6524-6550" color="#a020f0" type="standard"/><Q name="note"><V text="P364 FTL0702cmp_F"/></Q></Feature><Feature recentID="42" name="glyA" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6246" color="#a6acb3" type="standard"/><Q name="gene"><V text="glyA"/></Q><Q name="db_xref"><V text="3951072" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0703"/></Q></Feature><Feature recentID="41" name="glyA" directionality="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6265" maxFusedRunOn="6265"><Segment range="6113-6246" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="EC_number"><V text="2.1.2.1"/></Q><Q name="protein_id"><V text="YP_513454.1"/></Q><Q name="product"><V text="serine hydroxymethyltransferase"/></Q><Q name="gene"><V text="glyA"/></Q><Q name="inference"><V bool="1" text="2004" predef="profile"/></Q><Q name="note"><V text="catalyzes the reaction of glycine with 5,10-methylenetetrahydrofolate to form L-serine and tetrahydrofolate"/><V text="/dnas_title=serine hydroxymethyltransferase"/></Q><Q name="db_xref"><V text="89256092" predef="GI"/><V text="3951072" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0703"/></Q></Feature><Feature recentID="40" name="RPp4" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6506-6532" color="#a6acb3" type="standard"/><Q name="note"><V text="RPp4"/></Q></Feature><Feature recentID="39" name="PRP12" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6345-6374" color="#a020f0" type="standard"/><Q name="note"><V text="PRP12"/></Q></Feature><Feature recentID="38" name="200 bp max AT content (91%)" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6306-6505" color="#a6acb3" type="standard"/><Q name="note"><V text="200 bp max AT content (91%)"/></Q></Feature><Feature recentID="37" name="promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6474-6474" color="#ffffff" type="standard"/><Q name="note"><V text="Potential TSS"/></Q></Feature><Feature recentID="36" name="Potential -10 element" type="-10_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6462-6467" color="#a6acb3" type="standard"/><Q name="note"><V text="Potential -10 element"/></Q></Feature><Feature recentID="35" name="Potential -35 element" type="-35_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6440-6445" color="#a6acb3" type="standard"/><Q name="note"><V text="Potential -35 element"/></Q></Feature><Feature recentID="34" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6345-6374" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P431 P_FTL0702_F"/></Q></Feature><Feature recentID="33" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6473-6506" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P434 FTL0702cmp_F2"/></Q></Feature><Feature recentID="32" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6216-6237" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P478 RT_up0702-1_F"/></Q></Feature><Feature recentID="31" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6223-6249" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P480 RT_up0702-2_F"/></Q></Feature><Feature recentID="30" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6151-6175" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P482 RT_up0702-3_F"/></Q></Feature><Feature recentID="29" name="P691 PriM_mpk_F1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6134" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P691 PriM_mpk_F1"/></Q></Feature><Feature recentID="28" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-8239" color="#ffffff" type="standard"/><Q name="mol_type"><V predef="genomic DNA"/></Q><Q name="strain"><V text="LVS"/></Q><Q name="note"><V text="/dnas_title=pKL115 pEX_PriM_mpk1B"/></Q><Q name="db_xref"><V text="119857" predef="taxon"/></Q><Q name="sub_species"><V text="holarctica"/></Q><Q name="organism"><V text="Francisella tularensis subsp. holarctica"/></Q></Feature><Feature recentID="27" name="P367 FTL0702seqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6831-6855" color="#a020f0" type="standard"/><Q name="note"><V text="P367 FTL0702seqF1"/></Q></Feature><Feature recentID="26" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6928-6954" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P429 FTL0702_VSVG_2R"/></Q></Feature><Feature recentID="25" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7602-7626" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="24" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7116-7135" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="23" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7214-7235" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="22" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7331-7354" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="21" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7399-7418" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="20" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7439-7465" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="19" name="FTL_0701" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="8046-8239" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3951070" predef="GeneID"/></Q><Q name="locus_                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                n       !        !Л.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAQb^MjZ RXW9RSI[nZ)jjnZ]kmiiYojm]JfffUVVUZifjVWVYjUԵuVQ^luJᆥcUh(GF8guk}jckU6M8cRYMXUB^]`)F|^UF׎;PY8TF]UTQZC$yU&yVFoBlUS8Ql*YUYX/2jmڕ69^bGhf+4#M$X-f]TcxqyyyiK$uM*M8cwftd1I"UVN\;ZSET9ityeU8~gwy&z~FV^zTӝX֑Bdrgbnu$(cmC8Eju5%!%V6%tӕY%9:tduBԦ|Rft'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ                      !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  X<?xml version="1.0"?><Features recycledIDs="7,18,10,11,13,14,15,16,17,3,55,50,48" nextValidID="76"><Feature recentID="75" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7978-7994" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="74" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7954-7970" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="73" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="72" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8047-8068" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="71" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="70" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8002-8008" color="#ffffff" type="standard"/><Segment range="8009-8026" color="#ffffff" type="standard"/><Segment name="-35" range="8027-8032" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="69" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="68" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="67" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="66" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="65" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8356-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="64" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="63" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="62" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="61" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="60" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="59" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="58" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="57" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7965-7990" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="56" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7987-8006" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="54" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="53" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="52" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="51" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="49" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="47" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="46" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="45" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6524-7927" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3951071" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0702"/></Q></Feature><Feature recentID="44" name="FTL_0702" directionality="1" translationMW="51048.96" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7949" maxFusedRunOn="7949"><Segment range="6519-7927" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="YP_513453.1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="inference"><V predef="ab initio prediction"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="db_xref"><V text="89256091" predef="GI"/><V text="3951071" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0702"/></Q></Feature><Feature recentID="43" name="P364 FTL0702cmp_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6524-6550" color="#a020f0" type="standard"/><Q name="note"><V text="P364 FTL0702cmp_F"/></Q></Feature><Feature recentID="42" name="glyA" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6246" color="#a6acb3" type="standard"/><Q name="gene"><V text="glyA"/></Q><Q name="db_xref"><V text="3951072" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0703"/></Q></Feature><Feature recentID="41" name="glyA" directionality="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6265" maxFusedRunOn="6265"><Segment range="6113-6246" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="EC_number"><V text="2.1.2.1"/></Q><Q name="protein_id"><V text="YP_513454.1"/></Q><Q name="product"><V text="serine hydroxymethyltransferase"/></Q><Q name="gene"><V text="glyA"/></Q><Q name="inference"><V bool="1" text="2004" predef="profile"/></Q><Q name="note"><V text="catalyzes the reaction of glycine with 5,10-methylenetetrahydrofolate to form L-serine and tetrahydrofolate"/><V text="/dnas_title=serine hydroxymethyltransferase"/></Q><Q name="db_xref"><V text="89256092" predef="GI"/><V text="3951072" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0703"/></Q></Feature><Feature recentID="40" name="RPp4" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6506-6532" color="#a6acb3" type="standard"/><Q name="note"><V text="RPp4"/></Q></Feature><Feature recentID="39" name="PRP12" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6345-6374" color="#a020f0" type="standard"/><Q name="note"><V text="PRP12"/></Q></Feature><Feature recentID="38" name="200 bp max AT content (91%)" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6306-6505" color="#a6acb3" type="standard"/><Q name="note"><V text="200 bp max AT content (91%)"/></Q></Feature><Feature recentID="37" name="promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6474-6474" color="#ffffff" type="standard"/><Q name="note"><V text="Potential TSS"/></Q></Feature><Feature recentID="36" name="Potential -10 element" type="-10_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6462-6467" color="#a6acb3" type="standard"/><Q name="note"><V text="Potential -10 element"/></Q></Feature><Feature recentID="35" name="Potential -35 element" type="-35_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6440-6445" color="#a6acb3" type="standard"/><Q name="note"><V text="Potential -35 element"/></Q></Feature><Feature recentID="34" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6345-6374" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P431 P_FTL0702_F"/></Q></Feature><Feature recentID="33" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6473-6506" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P434 FTL0702cmp_F2"/></Q></Feature><Feature recentID="32" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6216-6237" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P478 RT_up0702-1_F"/></Q></Feature><Feature recentID="31" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6223-6249" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P480 RT_up0702-2_F"/></Q></Feature><Feature recentID="30" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6151-6175" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P482 RT_up0702-3_F"/></Q></Feature><Feature recentID="29" name="P691 PriM_mpk_F1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6134" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P691 PriM_mpk_F1"/></Q></Feature><Feature recentID="28" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-7932" color="#ffffff" type="standard"/><Q name="mol_type"><V predef="genomic DNA"/></Q><Q name="strain"><V text="LVS"/></Q><Q name="note"><V text="/dnas_title=pKL115 pEX_PriM_mpk1B"/></Q><Q name="db_xref"><V text="119857" predef="taxon"/></Q><Q name="sub_species"><V text="holarctica"/></Q><Q name="organism"><V text="Francisella tularensis subsp. holarctica"/></Q></Feature><Feature recentID="27" name="P367 FTL0702seqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6831-6855" color="#a020f0" type="standard"/><Q name="note"><V text="P367 FTL0702seqF1"/></Q></Feature><Feature recentID="26" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6928-6954" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P429 FTL0702_VSVG_2R"/></Q></Feature><Feature recentID="25" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7602-7626" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="24" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7116-7135" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="23" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7214-7235" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="22" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7331-7354" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="21" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7399-7418" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="20" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7439-7465" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="19" name="FTL_0701" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7928-7932" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3951070" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0701"/></Q></Feature><Feature recentID="12" name="P368 FTL0702seqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7671-7699" color="#a020f0" type="standard"/><Q name="note"><V text="P368 FTL0702seqR1"/></Q></Feature><Feature recentID="9" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7749-7771" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P437 RT_FTL0702-2F"/></Q></Feature><Feature recentID="8" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7854-7875" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P438 RT_FTL0702-2R"/></Q></Feature><Feature recentID="6" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7151-7201" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="5" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7178-7230" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P693 PriM_mpk_F2"/></Q></Feature><Feature recentID="4" name="Altered region!" type="misc_difference" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7173-7328" color="#ff0000" type="standard"/><Q name="note"><V text="Altered region!"/></Q></Feature><Feature recentID="2" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7324-7359" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P696 PriM_mpk_F4"/></Q></Feature><Feature recentID="1" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7592-7614" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P698 Ck_PriM_mpk1R"/></Q></Feature><Feature recentID="0" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7116-7139" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature></Features>
  
<?xml version="1.0"?><Primers nextValidID="4"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="3" name="P819 PriM_mpk_R2" sequence="atatGGTACCtgtaaagccaaatatactttctcttcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7900-7932" boundStrand="1" annealedBases="GGTACCtgtaaagccaaatatactttctcttcg" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="7900-7932" bases="GGTACCtgtaaagccaaatatactttctcttcg"/></BindingSite><BindingSite simplified="1" location="7900-7932" boundStrand="1" annealedBases="GGTACCtgtaaagccaaatatactttctcttcg" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="7900-7932" bases="GGTACCtgtaaagccaaatatactttctcttcg"/></BindingSite></Primer><Primer recentID="2" name="P696 PriM_mpk_F4" sequence="ttcactaGCAgctgatgctaacacacttacatatac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7318-7353" boundStrand="0" annealedBases="ttcactaGCAgctgatgctaacacacttacatatac" meltingTemperature="62"><Component hybridizedRange="7318-7353" bases="ttcactaGCAgctgatgctaacacacttacatatac"/></BindingSite><BindingSite simplified="1" location="7318-7353" boundStrand="0" annealedBases="ttcactaGCAgctgatgctaacacacttacatatac" meltingTemperature="62"><Component hybridizedRange="7318-7353" bases="ttcactaGCAgctgatgctaacacacttacatatac"/></BindingSite></Primer><Primer recentID="1" name="P695 PriM_mpk_R3" sequence="catcagcTGCtagtgaaactgctttagttgtatcact" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7298-7334" boundStrand="1" annealedBases="catcagcTGCtagtgaaactgctttagttgtatcact" meltingTemperature="64"><Component hybridizedRange="7298-7334" bases="catcagcTGCtagtgaaactgctttagttgtatcact"/></BindingSite><BindingSite simplified="1" location="7298-7334" boundStrand="1" annealedBases="catcagcTGCtagtgaaactgctttagttgtatcact" meltingTemperature="64"><Component hybridizedRange="7298-7334" bases="catcagcTGCtagtgaaactgctttagttgtatcact"/></BindingSite></Primer><Primer recentID="0" name="P818 PriM_mpk_F2" sequence="atatGGATCCaattgtaaatgaatctgttactaaatattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6595-6624" boundStrand="0" annealedBases="aattgtaaatgaatctgttactaaatattc" meltingTemperature="52"><Component bases="atatGGATCC"/><Component hybridizedRange="6595-6624" bases="aattgtaaatgaatctgttactaaatattc"/></BindingSite><BindingSite simplified="1" location="6595-6624" boundStrand="0" annealedBases="aattgtaaatgaatctgttactaaatattc" meltingTemperature="52"><Component bases="atatGGATCC"/><Component hybridizedRange="6595-6624" bases="aattgtaaatgaatctgttactaaatattc"/></BindingSite></Primer></Primers>
  A<Notes>
<UUID>c612d86d-1ad4-4bc9-9393-f55b37023277</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:22:37">2018.7.5</Created>
<LastModified UTC="23:59:52">2018.7.5</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  {'        #        #.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAQb^MjZ RXW9RSI[nZ)jjnZ]kmiiYojm]JfffUVVUZifjVWVYjUԵuVQ^luJᆥcUh(GF8guk}jckU6M8cRYMXUB^]`)F|^UF׎;PY8TF]UTQZC$yU&yVFoBlUS8Ql*YUYX/2jmڕ69^bGhf+4#M$X-f]TcxqyyyiK$uM*M8cwftd1I"UVN\;ZSET9ityeU8~gwy&z~FV^zTӝX֑Bdrgbnu$(cmC8Eju5%!%V6%tӕY%9:tduBԦ|Rfu*zRX>TQayajZZNެwcYueYj٨IjDgJIm:ڍM9حk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>                     )  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  d<?xml version="1.0"?><Features recycledIDs="3,55,50,48" nextValidID="76"><Feature recentID="75" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8285-8301" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="74" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8261-8277" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="73" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="72" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8354-8375" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="71" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="70" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8309-8315" color="#ffffff" type="standard"/><Segment range="8316-8333" color="#ffffff" type="standard"/><Segment name="-35" range="8334-8339" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="69" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="68" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="67" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="66" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="65" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8663-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="64" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="63" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="62" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="61" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="60" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="59" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="58" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="57" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8272-8297" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="56" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8294-8313" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="54" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="53" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="52" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="51" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="49" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="47" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="46" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="45" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6524-7981" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3951071" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0702"/></Q></Feature><Feature recentID="44" name="FTL_0702" directionality="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7988" maxFusedRunOn="7988"><Segment range="6519-7981" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="YP_513453.1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="inference"><V predef="ab initio prediction"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="db_xref"><V text="89256091" predef="GI"/><V text="3951071" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0702"/></Q></Feature><Feature recentID="43" name="P364 FTL0702cmp_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6524-6550" color="#a020f0" type="standard"/><Q name="note"><V text="P364 FTL0702cmp_F"/></Q></Feature><Feature recentID="42" name="glyA" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6246" color="#a6acb3" type="standard"/><Q name="gene"><V text="glyA"/></Q><Q name="db_xref"><V text="3951072" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0703"/></Q></Feature><Feature recentID="41" name="glyA" directionality="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6265" maxFusedRunOn="6265"><Segment range="6113-6246" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="EC_number"><V text="2.1.2.1"/></Q><Q name="protein_id"><V text="YP_513454.1"/></Q><Q name="product"><V text="serine hydroxymethyltransferase"/></Q><Q name="gene"><V text="glyA"/></Q><Q name="inference"><V bool="1" text="2004" predef="profile"/></Q><Q name="note"><V text="catalyzes the reaction of glycine with 5,10-methylenetetrahydrofolate to form L-serine and tetrahydrofolate"/><V text="/dnas_title=serine hydroxymethyltransferase"/></Q><Q name="db_xref"><V text="89256092" predef="GI"/><V text="3951072" predef="GeneID"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0703"/></Q></Feature><Feature recentID="40" name="RPp4" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6506-6532" color="#a6acb3" type="standard"/><Q name="note"><V text="RPp4"/></Q></Feature><Feature recentID="39" name="PRP12" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6345-6374" color="#a020f0" type="standard"/><Q name="note"><V text="PRP12"/></Q></Feature><Feature recentID="38" name="200 bp max AT content (91%)" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6306-6505" color="#a6acb3" type="standard"/><Q name="note"><V text="200 bp max AT content (91%)"/></Q></Feature><Feature recentID="37" name="promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6474-6474" color="#ffffff" type="standard"/><Q name="note"><V text="Potential TSS"/></Q></Feature><Feature recentID="36" name="Potential -10 element" type="-10_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6462-6467" color="#a6acb3" type="standard"/><Q name="note"><V text="Potential -10 element"/></Q></Feature><Feature recentID="35" name="Potential -35 element" type="-35_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6440-6445" color="#a6acb3" type="standard"/><Q name="note"><V text="Potential -35 element"/></Q></Feature><Feature recentID="34" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6345-6374" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P431 P_FTL0702_F"/></Q></Feature><Feature recentID="33" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6473-6506" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P434 FTL0702cmp_F2"/></Q></Feature><Feature recentID="32" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6216-6237" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P478 RT_up0702-1_F"/></Q></Feature><Feature recentID="31" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6223-6249" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P480 RT_up0702-2_F"/></Q></Feature><Feature recentID="30" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6151-6175" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P482 RT_up0702-3_F"/></Q></Feature><Feature recentID="29" name="P691 PriM_mpk_F1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6134" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P691 PriM_mpk_F1"/></Q></Feature><Feature recentID="28" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-8239" color="#ffffff" type="standard"/><Q name="mol_type"><V predef="genomic DNA"/></Q><Q name="strain"><V text="LVS"/></Q><Q name="note"><V text="/dnas_title=pKL115 pEX_PriM_mpk1B"/></Q><Q name="db_xref"><V text="119857" predef="taxon"/></Q><Q name="sub_species"><V text="holarctica"/></Q><Q name="organism"><V text="Francisella tularensis subsp. holarctica"/></Q></Feature><Feature recentID="27" name="P367 FTL0702seqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6831-6855" color="#a020f0" type="standard"/><Q name="note"><V text="P367 FTL0702seqF1"/></Q></Feature><Feature recentID="26" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6928-6954" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P429 FTL0702_VSVG_2R"/></Q></Feature><Feature recentID="25" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7602-7626" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="24" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7116-7135" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="23" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7214-7235" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="22" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7331-7354" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="21" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7399-7418" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="20" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7439-7465" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="19" name="FTL_0701" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="8046-8239" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3951070" predef="GeneID"/></Q><Q name="locus_                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                7zXZ  ִF !   t/U'^] ˇf{3(1v%f M*	mj)p80*i	NY]QF7&!nx_-Z|2s]mؒ%@͖%r^;>Pa`y!#W%ߡQʎG㵡,BY⮰bZmB#<6ViC,V?Tݭ(䨽?Y_1y&ʐ~Md鳟{nSw*a7`T
hyFbU ha?b6:85٥璋>.)}>8T.OW򜢆<U.5p`R*FdUPEDɆbW~;Z.$WGi/@kxj0iU;хf~5)D|&C|tWTA/n&#H؜3@FX4W	!9F1 D^&h^ڦ">+F-[R;kSbtŃyj}
iC*ejڃ ar̯pQa/f3DҾe:tN>jrk3PXA:~Q&#nQ^X#{׼J7@\/>пGohenXa1;ZߡhđvxŧgNIw1:)g`-[^<2߳`36@v3Q>~j'i1!6Kq0ni,	S
B|pvbg`<hdǽ[agamXxTz43@=N#q jAGWֈ2P^4酼xN/z|-ҽ͢F!&Eҝ'AZy"6l܎cezʏEp|h`(@F7$
hE$@71CۃyzhXIoGTq|S)tvZ0V<]$cŽir.[tSa/QO
11T{JxɃi5@5HGe*w? 
oZLcG
y=Gxjmc	 Ж,TGf[c}fj聘L:VNk
gR;VveA2K.pjT|}ڢH~o+;Sיw8zl?
p~WmFBq̀gR6CHsH+ݯro"+lw=&@	_83]BYPAhKl+8;-LA7D)$פXa
em&؀ýh|D*gNa1%;WuBQo,G4;p5#h'Ft[UJjMwf~y~dZTskE5"g:;{ȃe"u)'9T^y|y5vY2IOFJ$`Wk.CWakKo$gbo/t' 09yXBtF4;9suLāv]jLW7{ᖳ}?J0bT"
UY$?uSd=4 KnO~(AgLH>T>Ǝ͐|*Ju-["VGUW.~qddwJ6kӈ5CLGKBR>(uC>wp2\A_Okl
`jp U(JzOî32#,HtrmؘI4OH-䷵ЗOKGyl ǕQ<,M?E˗KoyEJӞNT包 J\*˵}3]{-:<*lU[[%dDlyv1}5j517O'"dDad5"!8\xK2vJ#yR`:G*GnV ]KFtH#ږ[zղzzWp
ѣǙњЂ/ncGlB{1떙eg1;Y]&`0>JE
-;+5W?]g5Wbd6Z`Ǩrfg֨`'l5Vaf54U1-1~nݵw$i2ZjNs<q~lӕ
-en	J8ҤLdBad'.={'~o[*RVʍqŭgym<`!aOQ||	r65jǰ]wN.l'AE<3>tݣ6ȪzN)/>2PDg_,	7",pQV0Il}A-!~ZaYk"_b\@/oRb	E*q(xqOf69ּV[sOCHEư޾PFOfSeWu1!ׇn]Ǵz([YiLz|)~.d-ﱚb̏4c-2h~t8E}5q' @;'ms L.H}mQ -+Tv.3/c1Yl?Ӥ*̫?8$mR[#:B	5om#Z6-G*#sNcdo2dCw:uL    ?E  Tݱg    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  I<?xml version="1.0"?><Features nextValidID="48"><Feature recentID="0" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6628-6651" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature><Feature recentID="1" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7104-7126" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P698 Ck_PriM_mpk1R"/></Q></Feature><Feature recentID="2" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6836-6871" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P696 PriM_mpk_F4"/></Q></Feature><Feature recentID="3" name="Altered region!" type="misc_difference" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6685-6840" color="#ff0000" type="standard"/><Q name="note"><V text="Altered region!"/></Q></Feature><Feature recentID="4" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6690-6742" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P693 PriM_mpk_F2"/></Q></Feature><Feature recentID="5" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6663-6713" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="6" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7366-7387" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P438 RT_FTL0702-2R"/></Q></Feature><Feature recentID="7" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7261-7283" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P437 RT_FTL0702-2F"/></Q></Feature><Feature recentID="8" name="P368 FTL0702seqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7183-7211" color="#a020f0" type="standard"/><Q name="note"><V text="P368 FTL0702seqR1"/></Q></Feature><Feature recentID="9" name="FTL_0701" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7440-7444" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3951070" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0701"/></Q></Feature><Feature recentID="10" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6951-6977" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6911-6930" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6843-6866" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="13" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6726-6747" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="14" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6628-6647" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="15" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7114-7138" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="16" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6440-6466" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P429 FTL0702_VSVG_2R"/></Q></Feature><Feature recentID="17" name="P367 FTL0702seqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6343-6367" color="#a020f0" type="standard"/><Q name="note"><V text="P367 FTL0702seqF1"/></Q></Feature><Feature recentID="18" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6108-7444" color="#ffffff" type="standard"/><Q name="db_xref"><V text="119857" predef="taxon"/></Q><Q name="mol_type"><V predef="genomic DNA"/></Q><Q name="note"><V text="/dnas_title=pKL115 pEX_PriM_mpk1B"/></Q><Q name="organism"><V text="Francisella tularensis subsp. holarctica"/></Q><Q name="strain"><V text="LVS"/></Q><Q name="sub_species"><V text="holarctica"/></Q></Feature><Feature recentID="19" name="FTL_0702" directionality="1" translationMW="48313.65" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="27" maxRunOn="7461" maxFusedRunOn="7461"><Segment range="6108-7439" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256091" predef="GI"/><V text="3951071" predef="GeneID"/></Q><Q name="inference"><V predef="ab initio prediction"/></Q><Q name="locus_tag"><V text="FTL_0702"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="protein_id"><V text="YP_513453.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IVNESVTKYSNNVETDADTNTNSPIYAFKSIAADASNIQANGEKLARGFVLTNNGAVKIPNSIKPKNMYMKDQATAAQGLEKYRAERDEINNNIKKLESQKKLGWRIKVVAEQAKLKSINTKIDILKGIENGDKAYAEEKIAQFNIVKNITVNGPKAYFNDIAKPVLSHLNAAWDSATSLNYVDYRSNIDMFAGAAVVLANGQYNINSKDAATAIEFNDITNYLTIQTIKSLQGSDTTKAVSLAADANTLTYTVTTIGDLSSVQKKIASPRTALSICEASLISIRTNTKVTTARNIINRLSNKKAVKPILRHLLNQTSIDDIILYDLDKLGDNWALKAATNAIRSTIGSDSILYVKGHTLMLISTSSMVDAFIKAIAENEIYKQVSDADRVLFGKNACNFTAAKNSNNPIVKAMIAASKQIAGQLPKGQVIDTVFEEKVYLAL"/></Q></Feature><Feature recentID="20" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6108-7439" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3951071" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0702"/></Q></Feature><Feature recentID="21" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="22" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="23" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="24" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="25" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="26" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="27" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="28" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7499-7518" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="29" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7477-7502" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="30" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="31" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="32" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="33" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="34" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="35" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MNIKKFAKQATVLTFTTALLAGGATQAFA,KETNQKPYKETYGISHITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK*"/></Q></Feature><Feature recentID="36" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature recentID="37" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7868-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="40" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="42" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="7514-7520" color="#ffffff" type="standard"/><Segment range="7521-7538" color="#ffffff" type="standard"/><Segment name="-35" range="7539-7544" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="43" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="44" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7559-7580" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="45" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="46" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7466-7482" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="47" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7490-7506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="8"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="P697" sequence="tgggattctgctactagtcttaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-03-29T17:20:08Z"><BindingSite location="6627-6650" boundStrand="0" annealedBases="tgggattctgctactagtcttaac" meltingTemperature="56"><Component hybridizedRange="6627-6650" bases="tgggattctgctactagtcttaac"/></BindingSite><BindingSite simplified="1" location="6627-6650" boundStrand="0" annealedBases="tgggattctgctactagtcttaac" meltingTemperature="56"><Component hybridizedRange="6627-6650" bases="tgggattctgctactagtcttaac"/></BindingSite></Primer><Primer recentID="1" name="P698" sequence="tagctgctttaagtgcccagttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-03-29T17:19:07Z"><BindingSite location="7103-7125" boundStrand="1" annealedBases="tagctgctttaagtgcccagttg" meltingTemperature="60"><Component hybridizedRange="7103-7125" bases="tagctgctttaagtgcccagttg"/></BindingSite><BindingSite simplified="1" location="7103-7125" boundStrand="1" annealedBases="tagctgctttaagtgcccagttg" meltingTemperature="60"><Component hybridizedRange="7103-7125" bases="tagctgctttaagtgcccagttg"/></BindingSite></Primer><Primer recentID="2" name="KROL7" sequence="TGTTGTGTGGAATTGTGAGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7498-7517" boundStrand="1" annealedBases="TGTTGTGTGGAATTGTGAGC" meltingTemperature="55"><Component hybridizedRange="7498-7517" bases="TGTTGTGTGGAATTGTGAGC"/></BindingSite><BindingSite simplified="1" location="7498-7517" boundStrand="1" annealedBases="TGTTGTGTGGAATTGTGAGC" meltingTemperature="55"><Component hybridizedRange="7498-7517" bases="TGTTGTGTGGAATTGTGAGC"/></BindingSite></Primer><Primer recentID="3" name="KROL6" sequence="CCCAGTCACGACGTTGTAAA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6036-6055" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAA" meltingTemperature="57"><Component hybridizedRange="6036-6055" bases="CCCAGTCACGACGTTGTAAA"/></BindingSite><BindingSite simplified="1" location="6036-6055" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAA" meltingTemperature="57"><Component hybridizedRange="6036-6055" bases="CCCAGTCACGACGTTGTAAA"/></BindingSite></Primer><Primer recentID="4" name="P819 PriM_mpk_R2" sequence="atatGGTACCtgtaaagccaaatatactttctcttcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7412-7444" boundStrand="1" annealedBases="GGTACCtgtaaagccaaatatactttctcttcg" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="7412-7444" bases="GGTACCtgtaaagccaaatatactttctcttcg"/></BindingSite><BindingSite simplified="1" location="7412-7444" boundStrand="1" annealedBases="GGTACCtgtaaagccaaatatactttctcttcg" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="7412-7444" bases="GGTACCtgtaaagccaaatatactttctcttcg"/></BindingSite></Primer><Primer recentID="5" name="P696 PriM_mpk_F4" sequence="ttcactaGCAgctgatgctaacacacttacatatac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6830-6865" boundStrand="0" annealedBases="ttcactaGCAgctgatgctaacacacttacatatac" meltingTemperature="62"><Component hybridizedRange="6830-6865" bases="ttcactaGCAgctgatgctaacacacttacatatac"/></BindingSite><BindingSite simplified="1" location="6830-6865" boundStrand="0" annealedBases="ttcactaGCAgctgatgctaacacacttacatatac" meltingTemperature="62"><Component hybridizedRange="6830-6865" bases="ttcactaGCAgctgatgctaacacacttacatatac"/></BindingSite></Primer><Primer recentID="6" name="P695 PriM_mpk_R3" sequence="catcagcTGCtagtgaaactgctttagttgtatcact" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6810-6846" boundStrand="1" annealedBases="catcagcTGCtagtgaaactgctttagttgtatcact" meltingTemperature="64"><Component hybridizedRange="6810-6846" bases="catcagcTGCtagtgaaactgctttagttgtatcact"/></BindingSite><BindingSite simplified="1" location="6810-6846" boundStrand="1" annealedBases="catcagcTGCtagtgaaactgctttagttgtatcact" meltingTemperature="64"><Component hybridizedRange="6810-6846" bases="catcagcTGCtagtgaaactgctttagttgtatcact"/></BindingSite></Primer><Primer recentID="7" name="P818 PriM_mpk_F2" sequence="atatGGATCCaattgtaaatgaatctgttactaaatattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6101-6136" boundStrand="0" annealedBases="GGATCCaattgtaaatgaatctgttactaaatattc" meltingTemperature="57"><Component bases="atat"/><Component hybridizedRange="6101-6136" bases="GGATCCaattgtaaatgaatctgttactaaatattc"/></BindingSite><BindingSite simplified="1" location="6101-6136" boundStrand="0" annealedBases="GGATCCaattgtaaatgaatctgttactaaatattc" meltingTemperature="57"><Component bases="atat"/><Component hybridizedRange="6101-6136" bases="GGATCCaattgtaaatgaatctgttactaaatattc"/></BindingSite></Primer></Primers>
  A<Notes>
<UUID>c612d86d-1ad4-4bc9-9393-f55b37023277</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:22:37">2018.7.5</Created>
<LastModified UTC="17:20:8">2019.3.29</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         6+ Cutters                                                                                                   d     CHO  d              E              Y? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
