LOCUS       pKL04                   5634 bp    DNA     circular     21-JUL-2018
pEX18KanSequenced.fa -
FEATURES             Location/Qualifiers
     primer_bind     2117..2119
                     /note="P028 pEX18KanSeq4F (not entire 5' end)"
     primer_bind     2648..2674
                     /note="P031 pEX18KanSeq7F"
     primer_bind     3367..3391
                     /note="P033 pEX18KanSeq8F"
     primer_bind     4267..4290
                     /note="P023 pEX18SeqF2"
     CDS             complement(2120..2383)
                     /note="sacB"
     primer_bind     4..26
                     /note="P025 pEX18KanSeq1F"
     primer_bind     474..493
                     /note="P037 pEX18KanSeq9F"
     promoter        866..1103
                     /note="groES promotoer"
     primer_bind     733..755
                     /note="P026 pEX18KanSeq2F"
     CDS             1104..1916
                     /note="kanamycin resistance"
     primer_bind     complement(1583..1607)
                     /note="P027 pEX18KanSeq3F"
     rep_origin      complement(join(5334..5634,1..288))
                     /note="ColE origin"
     misc_feature    859..883
                     /note="P049 groES promoter F"
     primer_bind     4291..4310
                     /note="P021 pEX18SeqF1"
     primer_bind     complement(4960..4979)
                     /note="P022 pEX18SeqR1"
     primer_bind     complement(4938..4963)
                     /note="P024 pEX18SeqR2"
     primer_bind     complement(4873..4917)
                     /note="P001 FTL_1743fragR"
     sig_peptide     4882..4917
                     /note="VSVG"
     primer_bind     complement(4886..4914)
                     /note="P035 pEX18integrationVSVG"
     misc_feature    join(4376..5634,1..894)
                     /note="Contig 2556 (take1)"
     misc_feature    866..1107
                     /note="Contig 436 (take1)"
     misc_feature    1079..2820
                     /note="Contig 1410 (take1)"
     misc_feature    complement(2819..3061)
                     /note="Contig 678 (take1)"
     misc_feature    3054..3156
                     /note="Contig 2844 (take1)"
     misc_feature    3204..3294
                     /note="Contig 1856 (take1)"
     misc_feature    3306..3551
                     /note="Contig 2784 (take1)"
     misc_feature    complement(join(4376..5634,1..890))
                     /note="Contig 930 (take 2)"
     primer_bind     complement(5098..5121)
                     /note="P227 SP_pKL02_R"
     primer_bind     4889..4918
                     /note="P226 SP_pKL02_F"
     primer_bind     826..859
                     /note="P261 groESpCheck1"
     primer_bind     4376..4400
                     /note="P004 FTL_0851fragF"
     primer_bind     complement(4846..4872)
                     /note="P003 FTL_0851fragR"
     gene            4376..4872
                     /dnas_title="FTL_0851"
                     /db_xref="GeneID:3951139"
                     /label=FTL_0851
     CDS             4376..4872
                     /dnas_title="RNA polymerase factor sigma-32"
                     /label=FTL_0851
     source          1..5634
                     /dnas_title="pKL04 (pEX18kan_FTL0851VSVG_DsacB)"
ORIGIN      
        1 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT
       61 AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT
      121 AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC
      181 TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT
      241 TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG
      301 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
      361 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
      421 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAATC GATCAAGGGT TGGTTTGCGC
      481 ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC
      541 GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC
      601 GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG
      661 CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAATGATCGA AGTTAGGCTG
      721 GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC
      781 AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG
      841 AAGGCCATCC AGCCTCGCGT CGATCTATAC CCTTCAAGCT TTGAAAAATA AACTTAATTA
      901 TTATATATGT TATTTAGCTA GTTTTTTTAA TTAAAGTTAA AATCGAGAGC TTGTTTGACA
      961 AAAAAACAAA AAAATTTCTT GAAAATTTTT TTTTTGACTC AATATCTAGA CTTGCAAGAG
     1021 CTTGGAACTT TGAGATTGTT CTAAGATGCA TACAAATTCA AAATGCTTAA ACAAAAATAA
     1081 TTTAACAAAG GAGTAAGATT GTTATGAGCC ATATTCAACG GGAAACGTCT TGCTCGAGGC
     1141 CGCGATTAAA TTCCAACATG GATGCTGATT TATATGGGTA TAAATGGGCT CGCGATAATG
     1201 TCGGGCAATC AGGTGCGACA ATCTATCGAT TGTATGGGAA GCCCGATGCG CCAGAGTTGT
     1261 TTCTGAAACA TGGCAAAGGT AGCGTTGCCA ATGATGTTAC AGATGAGATG GTCAGACTAA
     1321 ACTGGCTGAC GGAATTTATG CCTCTTCCGA CCATCAAGCA TTTTATCCGT ACTCCTGATG
     1381 ATGCATGGTT ACTCACCACT GCGATCCCCG GAAAAACAGC ATTCCAGGTA TTAGAAGAAT
     1441 ATCCTGATTC AGGTGAAAAT ATTGTTGATG CGCTGGCAGT GTTCCTGCGC CGGTTGCATT
     1501 CGATTCCTGT TTGTAATTGT CCTTTTAACA GCGATCGCGT ATTTCGTCTC GCTCAGGCGC
     1561 AATCACGAAT GAATAACGGT TTGGTTGATG CGAGTGATTT TGATGACGAG CGTAATGGCT
     1621 GGCCTGTTGA ACAAGTCTGG AAAGAAATGC ATAAACTTTT GCCATTCTCA CCGGATTCAG
     1681 TCGTCACTCA TGGTGATTTC TCACTTGATA ACCTTATTTT TGACGAGGGG AAATTAATAG
     1741 GTTGTATTGA TGTTGGACGA GTCGGAATCG CAGACCGATA CCAGGATCTT GCCATCCTAT
     1801 GGAACTGCCT CGGTGAGTTT TCTCCTTCAT TACAGAAACG GCTTTTTCAA AAATATGGTA
     1861 TTGATAATCC TGATATGAAT AAATTGCAGT TTCATTTGAT GCTCGATGAG TTTTTCTAAT
     1921 CAGAATTGGT TAATTGGTTG TAACACTGGC AGAGCATTAC GCTGACTTGA CGGGACGGCG
     1981 GCTTTGTTGA ATAAATCGAA CTTTTGCTGA GTTGAAGGAT CAGATCACGC ATCTTCCCGA
     2041 CAACGCAGAC CGTTCCGTGG CAAAGCAAAA GTTCAAAATC ACCAACTGGT CCACCTACAA
     2101 CAAAGCTCTC ATCAACGATC CAGCTGTCCC AAACGTCCAG GCCTTTTGCA GAAGAGATAT
     2161 TTTTAATTGT GGACGAATCG AACTCAGGAA CTTGATATTT TTCATTTTTT TGCTGTTCAG
     2221 GGATTTGCAG CATATCATGG CGTGTAATAT GGGAAATGCC GTATGTTTCC TTATATGGCT
     2281 TTTGGTTCGT TTCTTTCGCA AACGCTTGAG TTGCGCCTCC TGCCAGCAGT GCGGTAGTAA
     2341 AGGTTAATAC TGTTGCTTGT TTTGCAAACT TTTTGATGTT CATCGTTCAT GTCTCCTTTT
     2401 TTATGTACTG TGTTAGCGGT CTGCTTCTTC CAGCCCTCCT GTTTGAAGAT GGCAAGTTAG
     2461 TTACGCACAA TAAAAAAAGA CCTAAAATAT GTAAGGGGTG ACGCCAAAGT ATACACTTTG
     2521 CCCTTTACAC ATTTTAGGTC TTGCCTGCTT TATCAGTAAC AAACCCGCGC GATTTACTTT
     2581 TCGACCTCAT TCTATTAGAC TCTCGTTTGG ATTGCAACTG GTCTATTTTC CTCTTTTGTT
     2641 TGATAGAAAA TCATAAAAGG ATTTGCAGAC TACGGGCCTA AAGAACTAAA AAATCTATCT
     2701 GTTTCTTTTC ATTCTCTGTA TTTTTTATAG TTTCTGTTGC ATGGGCATAA AGTTGCCTTT
     2761 TTAATCACAA TTCAGAAAAT ATCATAATAT CTCATTTCAC TAAATAATAG TGAACGGCAG
     2821 GTATATGTGA TGGGTTAAAA AGGATCGATC CTCTAGCTAG AGTCGATCTT CGCCAGCAGG
     2881 GCGAGGATCG TGGCATCACC GAACCGCGCC GTGCGCGGGT CGTCGGTGAG CCAGAGTTTC
     2941 AGCAGGCCGC CCAGGCGGCC CAGGTCGCCA TTGATGCGGG CCAGCTCGCG GACGTGCTCA
     3001 TAGTCCACGA CGCCCGTGAT TTTGTAGCCC TGGCCGACGG CCAGCAGGTA GGCCGACAGG
     3061 CTCATGCCGG CTGCCGCCGC CTTTTCCTCA ATCGCTCTTC GTTCGTCTGG AAGGCAGTAC
     3121 ACCTTGATAG GTGGGCTGCC CTTCCTGGTT GGCTTGGTTT CATCAGCCAT CCGCTTGCCC
     3181 TCATCTGTTA CGCCGGCGGT AGCCGGCCAG CCTCGCAGAG CAGGATTCCC GTTGAGCACC
     3241 GCCAGGTGCG AATAAGGGAC AGTGAAGAAG GAACACCCGC TCGCGGGTGG GCCTACTTCA
     3301 CCTATCCTGC CCGGCTGACG CCGTTGGATA CACCAAGGAA AGTCTACACG AACCCTTTGG
     3361 CAAAATCCTG TATATCGTGC GAAAAAGGAT GGATATACCG AAAAAATCGC TATAATGACC
     3421 CCGAAGCAGG GTTATGCAGC GGAAAAGCGC TGCTTCCCTG CTGTTTTGTG GAATATCTAC
     3481 CGACTGGAAA CAGGCAAATG CAGGAAATTA CTGAACTGAG GGGACAGGCG AGAGACGATG
     3541 CCAAAGAGCT ACACCGACGA GCTGGCCGAG TGGGTTGAAT CCCGCGCGGC CAAGAAGCGC
     3601 CGGCGTGATG AGGCTGCGGT TGCGTTCCTG GCGGTGAGGG CGGATGTCGA TATGCGGTGT
     3661 GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCATATT TGAA-TGTAT
     3721 TTAGAAAAAT AAACAAAAGA GTTTGTAGAA ACGCAAAAAG GCCATCCGTC AGGATGGCCT
     3781 TCTGCTTAAT TTGATGCCTG GCAGTTTATG GCGGGCGTCC TGCCCGCCAC CCTCCGGGCC
     3841 GTTGCTTCGC AACGTTCAAA TCCGCTCCCG GCGGATTTGT CCTACTCAGG AGAGCGTTCA
     3901 CCGACAAACA ACAGATAAAA CGAAAGGCCC AGTCTTTCGA CTGAGCCTTT CGTTTTATTT
     3961 GATGCCTGGC AGTTCCCTAC TCTCGCATGG GGAGACCCCA CACTACCATC GGCGCTACGG
     4021 CGTTTCACTT CTGAGTTCGG CATGGGGTCA GGTGGGACCA CCGCGCTACT GCCGCCAGGC
     4081 AAATTCTGTT TTATCAGACC GCTTCTGCGT TCTGATTTAA TCTGTATCAG GCTGAAAATC
     4141 TTCTCTCATC CGCCAAAACA GCCAAGCTCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG
     4201 GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC
     4261 TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC
     4321 GGCCAGTGCC AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GTACCactgg
     4381 aagatagtca aggttgctac cacaaaagca cagcgtaaat tattttttaa tcttagaagt
     4441 agtaaagaca aaattggttg gttaagctcg gagaatatca aagagttagc tgaagagcta
     4501 ggtgtcaaag aagaaactgt cattgagatg gagaaaagaa tgtgtcaggg ggatgcaagt
     4561 cttgatttat catacactga tgatgacggt gagcaaactt cgcagcaaag tttgtatcta
     4621 gaggataagt cttcaaacat tgagcatcaa gttgttcagc aagattacta tgataatttc
     4681 aaagcgattg taaaagatgt tttaagtggt tttgatacac gtacaaaaga tattatcatg
     4741 tcgcgctatc tacttgataa caaggcaact cttcaggatc tagctgctaa atataatatc
     4801 tcagccgaaa gagtacgcca aattgaagaa gatgctctag ctaagcttaa aaaagctatc
     4861 aagaatcgct ctGCGGCCGC aTATACTGAT ATTGAAATGA ATAGATTAGG TAAATAAGAA
     4921 TTCGTAATCA TGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC
     4981 AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC
     5041 ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG
     5101 CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT
     5161 TCCTCGCTCA CTGACCCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC
     5221 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA
     5281 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT
     5341 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC
     5401 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT
     5461 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG
     5521 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG
     5581 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAAC
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