LOCUS       Exported                5635 bp ds-DNA     circular SYN 21-JUL-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 21, 2018 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=pKL02 Verified"
     primer_bind     4..26
                     /label=P025 pEX18KanSeq1F
                     /note="P025 pEX18KanSeq1F"
     primer_bind     474..493
                     /label=P037 pEX18KanSeq9F
                     /note="P037 pEX18KanSeq9F"
     primer_bind     733..755
                     /label=P026 pEX18KanSeq2F
                     /note="P026 pEX18KanSeq2F"
     primer_bind     826..859
                     /label=P261 groESpCheck1
                     /note="P261 groESpCheck1"
     misc_feature    859..883
                     /label=P049 groES promoter F
                     /note="P049 groES promoter F"
     promoter        866..1103
                     /note="groES promotoer"
     CDS             1104..1916
                     /label=kanamycin resistance
                     /note="kanamycin resistance"
     misc_feature    1259..1283
                     /label=nonstandard type: PCR_primer
                     /note="P442 pEX_kanR_F"
     misc_feature    complement(1531..1553)
                     /label=nonstandard type: PCR_primer
                     /note="P443 pEX_kanR_R"
     primer_bind     complement(1583..1607)
                     /label=P027 pEX18KanSeq3F
                     /note="P027 pEX18KanSeq3F"
     misc_feature    complement(2007..2030)
                     /label=nonstandard type: PCR_primer
                     /note="P441 pEX_ckR"
     primer_bind     2117..2119
                     /label=P028 pEX18KanSeq4F (not entire 5' end)
                     /note="P028 pEX18KanSeq4F (not entire 5' end)"
     CDS             complement(2120..2383)
                     /label=sacB
                     /note="sacB"
     primer_bind     2648..2674
                     /label=P031 pEX18KanSeq7F
                     /note="P031 pEX18KanSeq7F"
     primer_bind     3367..3391
                     /label=P033 pEX18KanSeq8F
                     /note="P033 pEX18KanSeq8F"
     primer_bind     4266..4289
                     /label=P023 pEX18SeqF2
                     /note="P023 pEX18SeqF2"
     primer_bind     4290..4309
                     /label=P021 pEX18SeqF1
                     /note="P021 pEX18SeqF1"
     CDS             4375..4873
                     /label=FTL_1743 fragment
                     /note="FTL_1743 fragment"
     primer_bind     4375..4401
                     /label=P002 FTL_1743fragF
                     /note="P002 FTL_1743fragF"
     primer_bind     complement(4848..4918)
                     /label=P001 FTL_1743fragR
                     /note="P001 FTL_1743fragR"
     sig_peptide     4883..4918
                     /note="VSVG"
     primer_bind     complement(4887..4915)
                     /label=P035 pEX18integrationVSVG
                     /note="P035 pEX18integrationVSVG"
     primer_bind     4890..4919
                     /label=P226 SP_pKL02_F
                     /note="P226 SP_pKL02_F"
     primer_bind     complement(4939..4964)
                     /label=P024 pEX18SeqR2
                     /note="P024 pEX18SeqR2"
     primer_bind     complement(4961..4980)
                     /label=P022 pEX18SeqR1
                     /note="P022 pEX18SeqR1"
     primer_bind     complement(5099..5122)
                     /label=P227 SP_pKL02_R
                     /note="P227 SP_pKL02_R"
     rep_origin      complement(join(5335..5635,1..288))
                     /direction=LEFT
                     /label=ColE origin
                     /note="ColE origin"
ORIGIN
        1 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
       61 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
      121 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
      181 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
      241 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
      301 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
      361 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
      421 tcaatctaaa gtatatatga gtaaacttgg tctgacaatc gatcaagggt tggtttgcgc
      481 attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc
      541 gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac
      601 gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg
      661 ctcgccgagg cggcataaat cgccgtgacg atcagcggtc caatgatcga agttaggctg
      721 gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac
      781 agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg
      841 aaggccatcc agcctcgcgt cgatctatac ccttcaagct ttgaaaaata aacttaatta
      901 ttatatatgt tatttagcta gtttttttaa ttaaagttaa aatcgagagc ttgtttgaca
      961 aaaaaacaaa aaaatttctt gaaaattttt tttttgactc aatatctaga cttgcaagag
     1021 cttggaactt tgagattgtt ctaagatgca tacaaattca aaatgcttaa acaaaaataa
     1081 tttaacaaag gagtaagatt gttatgagcc atattcaacg ggaaacgtct tgctcgaggc
     1141 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
     1201 tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt
     1261 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     1321 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     1381 atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat
     1441 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     1501 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc
     1561 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct
     1621 ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag
     1681 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     1741 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     1801 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     1861 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat
     1921 cagaattggt taattggttg taacactggc agagcattac gctgacttga cgggacggcg
     1981 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
     2041 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
     2101 caaagctctc atcaacgatc cagctgtccc aaacgtccag gccttttgca gaagagatat
     2161 ttttaattgt ggacgaatcg aactcaggaa cttgatattt ttcatttttt tgctgttcag
     2221 ggatttgcag catatcatgg cgtgtaatat gggaaatgcc gtatgtttcc ttatatggct
     2281 tttggttcgt ttctttcgca aacgcttgag ttgcgcctcc tgccagcagt gcggtagtaa
     2341 aggttaatac tgttgcttgt tttgcaaact ttttgatgtt catcgttcat gtctcctttt
     2401 ttatgtactg tgttagcggt ctgcttcttc cagccctcct gtttgaagat ggcaagttag
     2461 ttacgcacaa taaaaaaaga cctaaaatat gtaaggggtg acgccaaagt atacactttg
     2521 ccctttacac attttaggtc ttgcctgctt tatcagtaac aaacccgcgc gatttacttt
     2581 tcgacctcat tctattagac tctcgtttgg attgcaactg gtctattttc ctcttttgtt
     2641 tgatagaaaa tcataaaagg atttgcagac tacgggccta aagaactaaa aaatctatct
     2701 gtttcttttc attctctgta ttttttatag tttctgttgc atgggcataa agttgccttt
     2761 ttaatcacaa ttcagaaaat atcataatat ctcatttcac taaataatag tgaacggcag
     2821 gtatatgtga tgggttaaaa aggatcgatc ctctagctag agtcgatctt cgccagcagg
     2881 gcgaggatcg tggcatcacc gaaccgcgcc gtgcgcgggt cgtcggtgag ccagagtttc
     2941 agcaggccgc ccaggcggcc caggtcgcca ttgatgcggg ccagctcgcg gacgtgctca
     3001 tagtccacga cgcccgtgat tttgtagccc tggccgacgg ccagcaggta ggccgacagg
     3061 ctcatgccgg ctgccgccgc cttttcctca atcgctcttc gttcgtctgg aaggcagtac
     3121 accttgatag gtgggctgcc cttcctggtt ggcttggttt catcagccat ccgcttgccc
     3181 tcatctgtta cgccggcggt agccggccag cctcgcagag caggattccc gttgagcacc
     3241 gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg gcctacttca
     3301 cctatcctgc ccggctgacg ccgttggata caccaaggaa agtctacacg aaccctttgg
     3361 caaaatcctg tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc tataatgacc
     3421 ccgaagcagg gttatgcagc ggaaaagcgc tgcttccctg ctgttttgtg gaatatctac
     3481 cgactggaaa caggcaaatg caggaaatta ctgaactgag gggacaggcg agagacgatg
     3541 ccaaagagct acaccgacga gctggccgag tgggttgaat cccgcgcggc caagaagcgc
     3601 cggcgtgatg aggctgcggt tgcgttcctg gcggtgaggg cggatgtcga tatgcggtgt
     3661 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgcatatt tgaatgtatt
     3721 tagaaaaata aacaaaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     3781 ctgcttaatt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     3841 ttgcttcgca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     3901 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg
     3961 atgcctggca gttccctact ctcgcatggg gagaccccac actaccatcg gcgctacggc
     4021 gtttcacttc tgagttcggc atggggtcag gtgggaccac cgcgctactg ccgccaggca
     4081 aattctgttt tatcagaccg cttctgcgtt ctgatttaat ctgtatcagg ctgaaaatct
     4141 tctctcatcc gccaaaacag ccaagctcgc cattcgccat tcaggctgcg caactgttgg
     4201 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     4261 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     4321 gccagtgcca agcttgcatg cctgcaggtc gactctagag gatccccggg taccagctgt
     4381 aattcttgat gaaggtgata gtaagtttgt caaagatgaa agtattgagt tagtaagaat
     4441 acttgaagaa aatgataaac tacgtaagca aggtaaaaaa gaagtagagt atgagctggt
     4501 attaatgggt attacacgtt cttctttatc tacggagtca ttcttatcgg ctgcttcttt
     4561 ccaagaaaca acaagagtgc ttactgaggc ttcgataaac tcacaaattg ataatttaag
     4621 aggacttaaa gaaaatgttc ttattggtag attaatacca gcaggtactg gtctagcagt
     4681 aagaaaagag tcagctaaaa ttgaaaaaat gcgtgaagaa ttaggggttg aagataatat
     4741 ggtgtttact gatttatcgt cttttaaccc tgaagaaatc tcttttgata gtattcaatc
     4801 tcaaaaagaa gataaagata ttaatgaaga catcgaggag tcattaagaa acgctcttga
     4861 gtcacttgat ttcgcggccg catatactga tattgaaatg aatagattag gtaaataaga
     4921 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     4981 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5041 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     5101 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5161 ttcctcgctc actgacccgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5221 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5281 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5341 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5401 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5461 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5521 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5581 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaac
//
