LOCUS       pEX18kan.rpoCTAP        7827 bp    DNA     circular SYN 24-Mar-2014
DEFINITION  pEX18kan.rpoCTAP
ACCESSION   pEX18kan.rpoCTAP
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     Textco/File created by Gene Construction Kit (Textco BioSoftware)
FEATURES             Location/Qualifiers
     misc_feature    6187..7451
     misc_feature    6187..7451
                     /label="pEX18kan"
     misc_feature    complement(6398..7213)
                     /label="kanamycin"
     CDS             complement(212..1630)
                     /label="sacB"
     CDS             2061..2822
                     /label="colE1"
     misc_feature    complement(7214..7451)
                     /label="groE promoter"
     misc_feature    4491..4566
                     /label="4278]"
     misc_feature    3574..3920
                     /label="Construct:  pEX18kan.rpoCTAP"
     CDS             3574..4479
                     /label="rpoC-TAP"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca  
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg  
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc  
      181 accataatcg gcattttctt ttgcgttttt atttgttaac tgttaattgt ccttgttcaa  
      241 ggatgctgtc tttgacaaca gatgttttct tgcctttgat gttcagcagg aagctaggcg  
      301 caaacgttga ttgtttgtct gcgtagaatc ctctgtttgt catatagctt gtaatcacga  
      361 cattgtttcc tttcgcttga ggtacagcga agtgtgagta agtaaaggtt acatcgttag  
      421 gatcaagatc catttttaac acaaggccag ttttgttcag cggcttgtat gggccagtta  
      481 aagaattaga aacataacca agcatgtaaa tatcgttaga cgtaatgccg tcaatcgtca  
      541 tttttgatcc gcgggagtca gtgaacagat accatttgcc gttcatttta aagacgttcg  
      601 cgcgttcaat ttcatctgtt actgtgttag atgcaatcag cggtttcatc acttttttca  
      661 gtgtgtaatc atcgtttagc tcaatcatac cgagagcgcc gtttgctaac tcagccgtgc  
      721 gttttttatc gctttgcaga agtttttgac tttcttgacg gaagaatgat gtgcttttgc  
      781 catagtatgc tttgttaaat aaagattctt cgccttggta gccatcttca gttccagtgt  
      841 ttgcttcaaa tactaagtat ttgtggcctt tatcttctac gtagtgagga tctctcagcg  
      901 tatggttgtc gcctgagctg tagttgcctt catcgatgaa ctgctgtaca ttttgatacg  
      961 tttttccgtc accgtcaaag attgatttat aatcctctac accgttgatg ttcaaagagc  
     1021 tgtctgatgc tgatacgtta acttgtgcag ttgtcagtgt ttgtttgccg taatgtttac  
     1081 cggagaaatc agtgtagaat aaacggattt ttccgtcaga tgtaaatgtg gctgaacctg  
     1141 accattcttg tgtttggtct tttaggatag aatcatttgc atcgaatttg tcgctgtctt  
     1201 taaagacgcg gccagcgttt ttccagctgt caatagaagt ttcgccgact ttttgataga  
     1261 acatgtaaat cgatgtgtca tccgcatttt taggatctcc ggctaatgca aagacgatgt  
     1321 ggtagccgtg atagtttgcg acagtgccgt cagcgttttg taatggccag ctgtcccaaa  
     1381 cgtccaggcc ttttgcagaa gagatatttt taattgtgga cgaatcgaac tcaggaactt  
     1441 gatatttttc atttttttgc tgttcaggga tttgcagcat atcatggcgt gtaatatggg  
     1501 aaatgccgta tgtttcctta tatggctttt ggttcgtttc tttcgcaaac gcttgagttg  
     1561 cgcctcctgc cagcagtgcg gtagtaaagg ttaatactgt tgcttgtttt gcaaactttt  
     1621 tgatgttcat cgttcatgtc tcctttttta tgtactgtgt tagcggtctg cttcttccag  
     1681 ccctcctgtt tgaagatggc aagttagtta cgcacaataa aaaaagacct aaaatatgta  
     1741 aggggtgacg ccaaagtata cactttgccc tttacacatt ttaggtcttg cctgctttat  
     1801 cagtaacaaa cccgcgcgat ttacttttcg acctcattct attagactct cgtttggatt  
     1861 gcaactggtc tattttcctc ttttgtttga tagaaaatca taaaaggatt tgcagactac  
     1921 gggcctaaag aactaaaaaa tctatctgtt tcttttcatt ctctgtattt tttatagttt  
     1981 ctgttgcatg ggcataaagt tgccttttta atcacaattc agaaaatatc ataatatctc  
     2041 atttcactaa ataatagtga acggcaggta tatgtgatgg gttaaaaagg atcgatcctc  
     2101 tagctagagt cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg  
     2161 cgcgggtcgt cggtgagcca gagtttcagc aggccgccca ggcggcccag gtcgccattg  
     2221 atgcgggcca gctcgcggac gtgctcatag tccacgacgc ccgtgatttt gtagccctgg  
     2281 ccgacggcca gcaggtaggc cgacaggctc atgccggccg ccgccgcctt ttcctcaatc  
     2341 gctcttcgtt cgtctggaag gcagtacacc ttgataggtg ggctgccctt cctggttggc  
     2401 ttggtttcat cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct  
     2461 cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa  
     2521 cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac  
     2581 caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga  
     2641 tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagcgctgc  
     2701 ttccctgctg ttttgtggaa tatctaccga ctggaaacag gcaaatgcag gaaattactg  
     2761 aactgagggg acaggcgaga gacgatgcca aagagctaca ccgacgagct ggccgagtgg  
     2821 gttgaatccc gcgcggccaa gaagcgccgg cgtgatgagg ctgcggttgc gttcctggcg  
     2881 gtgagggcgg atgtcgatat gcgtaaggag aaaataccgc atcaggcgca tatttgaatg  
     2941 tatttagaaa aataaacaaa aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg  
     3001 gccttctgct taatttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg  
     3061 ggccgttgct tcgcaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg  
     3121 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt  
     3181 atttgatgcc tggcagttcc ctactctcgc atggggagac cccacactac catcggcgct  
     3241 acggcgtttc acttctgagt tcggcatggg gtcaggtggg accaccgcgc tactgccgcc  
     3301 aggcaaattc tgttttatca gaccgcttct gcgttctgat ttaatctgta tcaggctgaa  
     3361 aatcttctct catccgccaa aacagccaag ctcgccattc gccattcagg ctgcgcaact  
     3421 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat  
     3481 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa  
     3541 cgacggccag tgccaagctt gcatgcctgc agcaattgGG AGTCATTCTT ATCGGCTGCT  
     3601 TCTTTCCAAG AAACAACAAG AGTGCTTACT GAGGCTTCGA TAAACTCACA AATTGATAAT  
     3661 TTAAGAGGAC TTAAAGAAAA TGTTCTTATT GGTAGATTAA TACCAGCAGG TACTGGTCTA  
     3721 GCAGTAAGAA AAGAGTCAGC TAAAATTGAA AAAATGCGTG AAGAATTAGG GGTTGAAGAT  
     3781 AATATGGTGT TTACTGATTT ATCGTCTTTT AACCCTGAAG AAATCTCTTT TGATAGTATT  
     3841 CAATCTCAAA AAGAAGATAA AGATATTAAT GAAGACATCG AGGAGTCATT AAGAAACGCT  
     3901 CTTGAGTCAC TTGATTTCgc GGCCGCATCC ATGGAAAAGA GAAGATGGAA AAAGAATTTC  
     3961 ATAGCCGTCT CAGCAGCCAA CCGCTTTAAG AAAATCTCAT CCTCCGGGGC ACTTGATTAT  
     4021 GATATTCCAA CTACTGCTAG CGAGAATTTG TATTTTCAGG GTGAGCTCAA AACCGCGGCT  
     4081 CTTGCGCAAC ACGATGAAGC CGTGGACAAC AAATTCAACA AAGAACAACA AAACGCGTTC  
     4141 TATGAGATCT TACATTTACC TAACTTAAAC GAAGAACAAC GAAACGCCTT CATCCAAAGT  
     4201 TTAAAAGATG ACCCAAGCCA AAGCGCTAAC CTTTTAGCAG AAGCTAAAAA GCTAAATGAT  
     4261 GCTCAGGCGC CGAAAGTAGA CAACAAATTC AACAAAGAAC AACAAAACGC GTTCTATGAG  
     4321 ATCTTACATT TACCTAACTT AAACGAAGAA CAACGAAACG CCTTCATCCA AAGTTTAAAA  
     4381 GATGACCCAA GCCAAAGCGC TAACCTTTTA GCAGAAGCTA AAAAGCTAAA TGGTGCTCAG  
     4441 GCGCCGAAAG TAGACGCGAA TTCCGCGGGG AAGTCAACCT GAGGATCCCC CCACCACGGC  
     4501 CCGGCCTGCC TTTCTGCAAT TAATTAATTT CCACGGGTGC GCATGATCGT GCTCCTGTCG  
     4561 TTGAGGctgc aggtcgactc tagaggatcc ccgggtaccg agctcgaatt cgtaatcatg  
     4621 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc  
     4681 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc  
     4741 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat  
     4801 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac  
     4861 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt  
     4921 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca  
     4981 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc  
     5041 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact  
     5101 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct  
     5161 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag  
     5221 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca  
     5281 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa  
     5341 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc  
     5401 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag  
     5461 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg  
     5521 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca  
     5581 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc  
     5641 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag  
     5701 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata  
     5761 tgagtaaact tggtctgaca atcgatgcaa gggttggttt gcgcattcac agttctccgc  
     5821 aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt  
     5881 ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa  
     5941 ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat  
     6001 aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg  
     6061 atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg  
     6121 cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc  
     6181 gcgtcgGGAT CCCCGCCACG GTTGATGAGA GCTTTGTTGT AGGTGGACCA GTTGGTGATT  
     6241 TTGAACTTTT GCTTTGCCAC GGAACGGTCT GCGTTGTCGG GAAGATGCGT GATCTGATCC  
     6301 TTCAACTCAG CAAAAGTTCG ATTTATTCAA CAAAGCCGCC GTCCCGTCAA GTCAGCGTAA  
     6361 TGCTCTGCCA GTGTTACAAC CAATTAACCA ATTCTGATTA GAAAAACTCA TCGAGCATCA  
     6421 AATGAAACTG CAATTTATTC ATATCAGGAT TATCAATACC ATATTTTTGA AAAAGCCGTT  
     6481 TCTGTAATGA AGGAGAAAAC TCACCGAGGC AGTTCCATAG GATGGCAAGA TCCTGGTATC  
     6541 GGTCTGCGAT TCCGACTCGT CCAACATCAA TACAACCTAT TAATTTCCCC TCGTCAAAAA  
     6601 TAAGGTTATC AAGTGAGAAA TCACCATGAG TGACGACTGA ATCCGGTGAG AATGGCAAAA  
     6661 GTTTATGCAT TTCTTTCCAG ACTTGTTCAA CAGGCCAGCC ATTACGCTCG TCATCAAAAT  
     6721 CACTCGCATC AACCAAACCG TTATTCATTC GTGATTGCGC CTGAGCGAGA CGAAATACGC  
     6781 GATCGCTGTT AAAAGGACAA TTACAAACAG GAATCGAATG CAACCGGCGC AGGAACACTG  
     6841 CCAGCGCATC AACAATATTT TCACCTGAAT CAGGATATTC TTCTAATACC TGGAATGCTG  
     6901 TTTTTCCGGG GATCGCAGTG GTGAGTAACC ATGCATCATC AGGAGTACGG ATAAAATGCT  
     6961 TGATGGTCGG AAGAGGCATA AATTCCGTCA GCCAGTTTAG TCTGACCATC TCATCTGTAA  
     7021 CATCATTGGC AACGCTACCT TTGCCATGTT TCAGAAACAA CTCTGGCGCA TCGGGCTTCC  
     7081 CATACAATCG ATAGATTGTC GCACCTGATT GCCCGACATT ATCGCGAGCC CATTTATACC  
     7141 CATATAAATC AGCATCCATG TTGGAATTTA ATCGCGGCCT CGAGCAAGAC GTTTCCCGTT  
     7201 GAATATGGCT CATAACAATC TTACTCCTTT GTTAAATTAT TTTTGTTTAA GCATTTTGAA  
     7261 TTTGTATGCA TCTTAGAACA ATCTCAAAGT TCCAAGCTCT TGCAAGTCTA GATATTGAGT  
     7321 CAAAAAAAAA ATTTTCAAGA AATTTTTTTG TTTTTTTGTC AAACAAGCTC TCGATTTTAA  
     7381 CTTTAATTAA AAAAACTAGC TAAATAACAT ATATAATAAT TAAGTTTATT TTTCAAAGCT  
     7441 TGAAGGGTAT Aatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca  
     7501 gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt  
     7561 gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagcta atcggttgaa  
     7621 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga  
     7681 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc  
     7741 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa  
     7801 ataggcgtat cacgaggccc tttcgtc 
//
