LOCUS       pEX                     6845 bp    DNA     circular     21-JUL-2018
pEX18KanSequenced.fa -
FEATURES             Location/Qualifiers
     primer_bind     2117..2132
                     /note="P028 pEX18KanSeq4F (not entire 5' end)"
     primer_bind     4390..4416
                     /note="P031 pEX18KanSeq7F"
     primer_bind     5113..5137
                     /note="P033 pEX18KanSeq8F"
     primer_bind     6013..6036
                     /note="P023 pEX18SeqF2"
     primer_bind     6037..6056
                     /note="P021 pEX18SeqF1"
     primer_bind     complement(6148..6173)
                     /note="P024 pEX18SeqR2"
     primer_bind     complement(6170..6189)
                     /note="P022 pEX18SeqR1"
     CDS             complement(2707..4125)
                     /note="sacB"
     primer_bind     4..26
                     /note="P025 pEX18KanSeq1F"
     primer_bind     474..493
                     /note="P037 pEX18KanSeq9F"
     promoter        866..1103
                     /note="groES promotoer"
     primer_bind     733..755
                     /note="P026 pEX18KanSeq2F"
     CDS             1104..1916
                     /note="kanamycin resistance"
     primer_bind     complement(1583..1607)
                     /note="P027 pEX18KanSeq3F"
     rep_origin      complement(join(6545..6845,1..288))
                     /note="ColE origin"
     primer_bind     2968..2994
                     /note="P029 pEX18KanSeq5F"
     primer_bind     3649..3675
                     /note="P030 pEX18KanSeq6F"
     PCR_primer      2636..2659
                     /note="P600 Seq_sacB1"
     PCR_primer      complement(4172..4194)
                     /note="P602 Seq_sacB3"
     PCR_primer      complement(3358..3382)
                     /note="P601 Seq_sacB2"
     source          1..6845
                     /dnas_title="pEX"
ORIGIN      
        1 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT
       61 AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT
      121 AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC
      181 TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT
      241 TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG
      301 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
      361 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
      421 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAATC GATCAAGGGT TGGTTTGCGC
      481 ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC
      541 GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC
      601 GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG
      661 CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAATGATCGA AGTTAGGCTG
      721 GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC
      781 AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG
      841 AAGGCCATCC AGCCTCGCGT CGATCTATAC CCTTCAAGCT TTGAAAAATA AACTTAATTA
      901 TTATATATGT TATTTAGCTA GTTTTTTTAA TTAAAGTTAA AATCGAGAGC TTGTTTGACA
      961 AAAAAACAAA AAAATTTCTT GAAAATTTTT TTTTTGACTC AATATCTAGA CTTGCAAGAG
     1021 CTTGGAACTT TGAGATTGTT CTAAGATGCA TACAAATTCA AAATGCTTAA ACAAAAATAA
     1081 TTTAACAAAG GAGTAAGATT GTTATGAGCC ATATTCAACG GGAAACGTCT TGCTCGAGGC
     1141 CGCGATTAAA TTCCAACATG GATGCTGATT TATATGGGTA TAAATGGGCT CGCGATAATG
     1201 TCGGGCAATC AGGTGCGACA ATCTATCGAT TGTATGGGAA GCCCGATGCG CCAGAGTTGT
     1261 TTCTGAAACA TGGCAAAGGT AGCGTTGCCA ATGATGTTAC AGATGAGATG GTCAGACTAA
     1321 ACTGGCTGAC GGAATTTATG CCTCTTCCGA CCATCAAGCA TTTTATCCGT ACTCCTGATG
     1381 ATGCATGGTT ACTCACCACT GCGATCCCCG GAAAAACAGC ATTCCAGGTA TTAGAAGAAT
     1441 ATCCTGATTC AGGTGAAAAT ATTGTTGATG CGCTGGCAGT GTTCCTGCGC CGGTTGCATT
     1501 CGATTCCTGT TTGTAATTGT CCTTTTAACA GCGATCGCGT ATTTCGTCTC GCTCAGGCGC
     1561 AATCACGAAT GAATAACGGT TTGGTTGATG CGAGTGATTT TGATGACGAG CGTAATGGCT
     1621 GGCCTGTTGA ACAAGTCTGG AAAGAAATGC ATAAACTTTT GCCATTCTCA CCGGATTCAG
     1681 TCGTCACTCA TGGTGATTTC TCACTTGATA ACCTTATTTT TGACGAGGGG AAATTAATAG
     1741 GTTGTATTGA TGTTGGACGA GTCGGAATCG CAGACCGATA CCAGGATCTT GCCATCCTAT
     1801 GGAACTGCCT CGGTGAGTTT TCTCCTTCAT TACAGAAACG GCTTTTTCAA AAATATGGTA
     1861 TTGATAATCC TGATATGAAT AAATTGCAGT TTCATTTGAT GCTCGATGAG TTTTTCTAAT
     1921 CAGAATTGGT TAATTGGTTG TAACACTGGC AGAGCATTAC GCTGACTTGA CGGGACGGCG
     1981 GCTTTGTTGA ATAAATCGAA CTTTTGCTGA GTTGAAGGAT CAGATCACGC ATCTTCCCGA
     2041 CAACGCAGAC CGTTCCGTGG CAAAGCAAAA GTTCAAAATC ACCAACTGGT CCACCTACAA
     2101 CAAAGCTCTC ATCAACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA
     2161 TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA
     2221 TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTAAT
     2281 CGGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG
     2341 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAGAAAT AAACAAATAG GGGTTCCGCG
     2401 CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC
     2461 CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA
     2521 AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG
     2581 GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA
     2641 CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT AATCGGCATT TTCTTTTGCG
     2701 TTTTTATTTG TTAACTGTTA ATTGTCCTTG TTCAAGGATG CTGTCTTTGA CAACAGATGT
     2761 TTTCTTGCCT TTGATGTTCA GCAGGAAGCT AGGCGCAAAC GTTGATTGTT TGTCTGCGTA
     2821 GAATCCTCTG TTTGTCATAT AGCTTGTAAT CACGACATTG TTTCCTTTCG CTTGAGGTAC
     2881 AGCGAAGTGT GAGTAAGTAA AGGTTACATC GTTAGGATCA AGATCCATTT TTAACACAAG
     2941 GCCAGTTTTG TTCAGCGGCT TGTATGGGCC AGTTAAAGAA TTAGAAACAT AACCAAGCAT
     3001 GTAAATATCG TTAGACGTAA TGCCGTCAAT CGTCATTTTT GATCCGCGGG AGTCAGTGAA
     3061 CAGATACCAT TTGCCGTTCA TTTTAAAGAC GTTCGCGCGT TCAATTTCAT CTGTTACTGT
     3121 GTTAGATGCA ATCAGCGGTT TCATCACTTT TTTCAGTGTG TAATCATCGT TTAGCTCAAT
     3181 CATACCGAGA GCGCCGTTTG CTAACTCAGC CGTGCGTTTT TTATCGCTTT GCAGAAGTTT
     3241 TTGACTTTCT TGACGGAAGA ATGATGTGCT TTTGCCATAG TATGCTTTGT TAAATAAAGA
     3301 TTCTTCGCCT TGGTAGCCAT CTTCAGTTCC AGTGTTTGCT TCAAATACTA AGTATTTGTG
     3361 GCCTTTATCT TCTACGTAGT GAGGATCTCT CAGCGTATGG TTGTCGCCTG AGCTGTAGTT
     3421 GCCTTCATCG ATGAACTGCT GTACATTTTG ATACGTTTTT CCGTCACCGT CAAAGATTGA
     3481 TTTATAATCC TCTACACCGT TGATGTTCAA AGAGCTGTCT GATGCTGATA CGTTAACTTG
     3541 TGCAGTTGTC AGTGTTTGTT TGCCGTAATG TTTACCGGAG AAATCAGTGT AGAATAAACG
     3601 GATTTTTCCG TCAGATGTAA ATGTGGCTGA ACCTGACCAT TCTTGTGTTT GGTCTTTTAG
     3661 GATAGAATCA TTTGCATCGA ATTTGTCGCT GTCTTTAAAG ACGCGGCCAG CGTTTTTCCA
     3721 GCTGTCAATA GAAGTTTCGC CGACTTTTTG ATAGAACATG TAAATCGATG TGTCATCCGC
     3781 ATTTTTAGGA TCTCCGGCTA ATGCAAAGAC GATGTGGTAG CCGTGATAGT TTGCGACAGT
     3841 GCCGTCAGCG TTTTGTAATG GCCAGCTGTC CCAAACGTCC AGGCCTTTTG CAGAAGAGAT
     3901 ATTTTTAATT GTGGACGAAT CGAACTCAGG AACTTGATAT TTTTCATTTT TTTGCTGTTC
     3961 AGGGATTTGC AGCATATCAT GGCGTGTAAT ATGGGAAATG CCGTATGTTT CCTTATATGG
     4021 CTTTTGGTTC GTTTCTTTCG CAAACGCTTG AGTTGCGCCT CCTGCCAGCA GTGCGGTAGT
     4081 AAAGGTTAAT ACTGTTGCTT GTTTTGCAAA CTTTTTGATG TTCATCGTTC ATGTCTCCTT
     4141 TTTTATGTAC TGTGTTAGCG GTCTGCTTCT TCCAGCCCTC CTGTTTGAAG ATGGCAAGTT
     4201 AGTTACGCAC AATAAAAAAA GACCTAAAAT ATGTAAGGGG TGACGCCAAA GTATACACTT
     4261 TGCCCTTTAC ACATTTTAGG TCTTGCCTGC TTTATCAGTA ACAAACCCGC GCGATTTACT
     4321 TTTCGACCTC ATTCTATTAG ACTCTCGTTT GGATTGCAAC TGGTCTATTT TCCTCTTTTG
     4381 TTTGATAGAA AATCATAAAA GGATTTGCAG ACTACGGGCC TAAAGAACTA AAAAATCTAT
     4441 CTGTTTCTTT TCATTCTCTG -TATTTTTTA TAGTTTCTGT TGCATGGGCA TAAAGTTGCC
     4501 TTTTTAATCA CAATTCAGAA AATATCATAA TATCTCATTT CACTAAATAA TAGTGAACGG
     4561 CAGGTATATG TGATGGGTTA AAAAGGATCG ATCCTCTAGC TAGAGTCGAT CTTCGCCAGC
     4621 AGGGCGAGGA TCGTGGCATC ACCGAA-CCG CGCCGTGCGC GGGTCGTCGG TGAGCCAGAG
     4681 TTTCAGCAGG CCGCCCAGGC GGCCCAGGTC GCCATTGATG CGGGCCAGCT CGCGGACGTG
     4741 CTCATAGTCC ACGACGCCCG TGATTTTGTA GCCCTGGCCG ACGGCCAGCA GGTAGGCCGA
     4801 CAGGCTCATG CCGGCTGCCG CCGCCTTTTC CTCAATCGCT CTTCGTTCGT CTGGAAGGCA
     4861 GTACACCTTG ATAGGTGGGC TGCCCTTCCT GGTTGGCTTG GTTTCATCAG CCATCCGCTT
     4921 GCCCTCATCT GTTA-CGCCG GCGGTAGCCG GCCAGCCTCG CAGAGCAGGA TTCCCGTTGA
     4981 GCACCGCCAG GTGCGAATAA GGGACAGTGA AGAAGGAACA CCCGCTCGCG GGTGGGCCTA
     5041 CTTCACCTA- TCCTGCCCGG CTGACGCCGT TGGATACACC AAGGAAAGTC TACACGAACC
     5101 CTTTGGCAAA ATCCTGTATA TCGTGCGAAA AAGGATGGAT ATACCGAAAA AATCGCTATA
     5161 ATGACCCCGA AGCAGGGTTA TGCAGCGGAA AAGCGCTGCT TCCCTGCTGT TTTGTGGAAT
     5221 ATCTACCGAC TGGAAACAGG CAAATGCAGG AAATTACTGA ACTGAGGGGA CAGGCGAGAG
     5281 ACGATGCCAA AGAGCTACAC CGACGAGCTG GCCGAGTGGG TTGAATCCCG CGCGGCCAAG
     5341 AAGCGCCGGC GTGATGAGGC TGCGGTTGCG TTCCTGGCGG TGAGGGCGGA TGTCGATATG
     5401 CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG CATATTTGAA
     5461 -TGTATTTAG AAAAATAAAC AAAAGAGTTT GTAGAAACGC AAAAAGGCCA TCCGTCAGGA
     5521 TGGCCTTCTG CTTAATTTGA TGCCTGGCAG TTTATGGCGG GCGTCCTGCC CGCCACCCTC
     5581 CGGGCCGTTG CTTCGCAACG TTCAAATCCG CTCCCGGCGG ATTTGTCCTA CTCAGGAGAG
     5641 CGTTCACCGA CAAACAACAG ATAAAACGAA AGGCCCAGTC TTTCGACTGA GCCTTTCGTT
     5701 TTATTTGATG CCTGGCAGTT CCCTACTCTC GCATGGGGAG ACCCCACACT ACCATCGGCG
     5761 CTACGGCGTT TCACTTCTGA GTTCGGCATG GGGTCAGGTG GGACCACCGC GCTACTGCCG
     5821 CCAGGCAAAT TCTGTTTTAT CAGACCGCTT CTGCGTTCTG ATTTAATCTG TATCAGGCTG
     5881 AAAATCTTCT CTCATCCGCC AAAACAGCCA AGCTCGCCAT TCGCCATTCA GGCTGCGCAA
     5941 CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCTGG CGAAAGGGGG
     6001 ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA
     6061 AACGACGGCC AGTGCCAAGC TTGCATGCCT GCAGGTCGAC TCTAGAGGAT CCCCGGGTAC
     6121 CGAGCTCGAA TTCGTAATCA TGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC
     6181 AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT
     6241 GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC
     6301 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG
     6361 CTCTTCCGCT -TCCTCGCTC ACTGACCCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG
     6421 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA
     6481 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG
     6541 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA
     6601 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG
     6661 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG
     6721 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC
     6781 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG
     6841 GTAAC
//

