Demultiplexing results: This is cutadapt 3.5 with Python 3.9.6 Command line parameters: -g ^TTTT --discard-untrimmed -o Input.fastq AM01-03_S1_L001_R1_001.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 27.51 s (4 µs/read; 13.76 M reads/minute). === Summary === Total reads processed: 6,308,834 Reads with adapters: 317,857 (5.0%) == Read fate breakdown == Reads discarded as untrimmed: 5,990,977 (95.0%) Reads written (passing filters): 317,857 (5.0%) Total basepairs processed: 319,791,754 bp Total written (filtered): 14,872,168 bp (4.7%) === Adapter 1 === Sequence: TTTT; Type: anchored 5'; Length: 4; Trimmed: 317857 times No. of allowed errors: 0 Overview of removed sequences length count expect max.err error counts 4 317857 24643.9 0 317857 This is cutadapt 3.5 with Python 3.9.6 Command line parameters: -g ^ACCA --discard-untrimmed -o Day7.fastq AM01-03_S1_L001_R1_001.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 32.24 s (5 µs/read; 11.74 M reads/minute). === Summary === Total reads processed: 6,308,834 Reads with adapters: 1,275,161 (20.2%) == Read fate breakdown == Reads discarded as untrimmed: 5,033,673 (79.8%) Reads written (passing filters): 1,275,161 (20.2%) Total basepairs processed: 319,791,754 bp Total written (filtered): 59,796,152 bp (18.7%) === Adapter 1 === Sequence: ACCA; Type: anchored 5'; Length: 4; Trimmed: 1275161 times No. of allowed errors: 0 Overview of removed sequences length count expect max.err error counts 4 1275161 24643.9 0 1275161 This is cutadapt 3.5 with Python 3.9.6 Command line parameters: -g ^ACGT --discard-untrimmed -o Day14.fastq AM01-03_S1_L001_R1_001.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 29.21 s (5 µs/read; 12.96 M reads/minute). === Summary === Total reads processed: 6,308,834 Reads with adapters: 918,587 (14.6%) == Read fate breakdown == Reads discarded as untrimmed: 5,390,247 (85.4%) Reads written (passing filters): 918,587 (14.6%) Total basepairs processed: 319,791,754 bp Total written (filtered): 43,071,119 bp (13.5%) === Adapter 1 === Sequence: ACGT; Type: anchored 5'; Length: 4; Trimmed: 918587 times No. of allowed errors: 0 Overview of removed sequences length count expect max.err error counts 4 918587 24643.9 0 918587