FastQCFastQC Report
Fri 11 Jun 2021
9_S19_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename9_S19_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14767916
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAAT10223336.922662615361571No Hit
GCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCC3454362.339097811769785No Hit
ATCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTA3110792.106451580575079No Hit
AGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATC2365721.6019321886717124No Hit
GGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATT2058011.3935683274471498No Hit
AGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTT1616821.094819336729705No Hit
TATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAA1590181.0767802308734693No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1347510.9124577902528698No Hit
GCTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTAT1180940.7996659785984699No Hit
GGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAACGC1153110.7808210718424996No Hit
CATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATGCG957700.6485004383827752No Hit
GTCACTTATACAATATACTTAGAAGCTTTTCCTGGAAGCATGGTATCAAT803560.5441255218407256No Hit
GCCCCGTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTAT795990.5389995446886344No Hit
ATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAACG711120.48153036623447754No Hit
CTTTTTTGTTGCCAGTTATTATTGGCATTTGTACTGTTATTAACGAGGTT639240.43285728331607526No Hit
CCACCAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAA597430.40454590884726055No Hit
GCTTTTTTGTTGCCAGTTATTATTGGCATTTGTACTGTTATTAACGAGGTT588790.3986953880290218No Hit
CTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATG559180.37864516564151635No Hit
CTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCA528010.3575385992173845No Hit
CACCAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAA498650.3376576627331846No Hit
GTCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTA458970.3107886041605329No Hit
CACTTATACAATATACTTAGAAGCTTTTCCTGGAAGCATGGTATCAATAG389900.2640182947952846No Hit
ATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATGCGGTATT387300.26225772140090725No Hit
CCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATACGGGA375270.25411168373384574No Hit
CTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCCAC372780.2524255961369228No Hit
CCCACCAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGA369170.24998110769319112No Hit
GCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTT363700.2462771321288664No Hit
GTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATACGGG323700.21919138759998363No Hit
GCTTAGGCTAATCTATTTGAGTTTAGCTTGACTATTCCCCAATTAGCCAGG319860.2165911561252109No Hit
GGTCACTTATACAATATACTTAGAAGCTTTTCCTGGAAGCATGGTATCAAT314670.21307678077258835No Hit
CCCCGTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATA308000.20856023287239714No Hit
GCAGGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGAT307670.20833677548003385No Hit
CTCAAATCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACT299460.2027774264154807No Hit
ATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATGCGG227300.15391474328537622No Hit
GCCACCTTTAATCCACAGATATTATGCGGTATTAACAGTCGTTTCCAA225510.1527026562177087No Hit
CCCGTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATACG218580.14801005097807976No Hit
AATCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCT218230.14777305071345206No Hit
TCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAAT215550.14595830583001693No Hit
CCCAGACAAAAAGCAAAGACCGTCTTATGCGGAATATGAACGGATGTAT204600.13854358326523528No Hit
CCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATGCGGT204080.13819146858635978No Hit
GCGGAATATGAACGGATGTATAGATAGTGTCATAAGAAAGACTATCATAT199880.1353474654108271No Hit
CAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCC198520.1344265500968451No Hit
GTCCAGATTACCGTTTTAGTTCAATATTCTTGATCCCTATGGTATCAAGGT184330.12481788222522394No Hit
GTTTGCTTTTTTGTTGCCAGTTATTATTGGCATTTGTACTGTTATTAACG174420.11810738901819322No Hit
TGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAACG160200.10847840683817542No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)