FastQCFastQC Report
Fri 11 Jun 2021
8_S18_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8_S18_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14790761
Sequences flagged as poor quality0
Sequence length35-51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAAT11583337.8314631681223155No Hit
GCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCC3737312.5267868232067303No Hit
ATCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTA3440002.325776205835521No Hit
AGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATC2607591.762985690864723No Hit
GGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATT2382601.6108704616348002No Hit
TATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAA1844281.2469135293309115No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1812991.2257584312260876No Hit
AGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTT1796021.2142850526757887No Hit
GCTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTAT1358820.9186951232597159No Hit
GGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAACGC1260180.8520048427528509No Hit
CATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATGCG1095590.7407259166719008No Hit
GTCACTTATACAATATACTTAGAAGCTTTTCCTGGAAGCATGGTATCAAT958780.648228985648541No Hit
GCCCCGTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTAT894330.6046544866758377No Hit
ATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAACG857010.5794225192334592No Hit
CCACCAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAA700630.4736943555507387No Hit
CTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATG662050.44761050496319965No Hit
CACCAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAA617790.417686419245095No Hit
CACTTATACAATATACTTAGAAGCTTTTCCTGGAAGCATGGTATCAATAG536860.3629698296118773No Hit
CTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCA536830.36294954667984963No Hit
GTCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTA505080.3414834436172689No Hit
CCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATACGGGA441180.2982807983984056No Hit
CTTTTTTGTTGCCAGTTATTATTGGCATTTGTACTGTTATTAACGAGGTT435390.29436619251707197No Hit
CCCACCAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGA433820.2933047190742924No Hit
GCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTT424650.2871049028511785No Hit
ATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATGCGGTATT419190.2834134092221489No Hit
CTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCCAC409210.2766659538342888No Hit
GCAGGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGAT408750.2763549488765318No Hit
GTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATACGGG396210.2678766832889802No Hit
GCTTTTTTGTTGCCAGTTATTATTGGCATTTGTACTGTTATTAACGAGGTT387400.26192026225019793No Hit
CCCCGTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATA371330.2510553716607279No Hit
CTCAAATCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACT370510.2505009715186392No Hit
GGTCACTTATACAATATACTTAGAAGCTTTTCCTGGAAGCATGGTATCAAT338900.22912952213885412No Hit
GCTTAGGCTAATCTATTTGAGTTTAGCTTGACTATTCCCCAATTAGCCAGG321840.21759529479247214No Hit
CCCGTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATACG267820.1810724951880434No Hit
TCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAAT247490.16732742825064917No Hit
ATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATGCGG245910.16625919383052704No Hit
AATCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCT229390.1550900592606425No Hit
CAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCC227930.1541029565686309No Hit
TGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAACG218250.14755833050104722No Hit
CCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATGCGGT210280.14216983155903878No Hit
GCCACCTTTAATCCACAGATATTATGCGGTATTAACAGTCGTTTCCAA208610.14104074834283375No Hit
CCCAGACAAAAAGCAAAGACCGTCTTATGCGGAATATGAACGGATGTAT197650.13363071717540428No Hit
GCGGAATATGAACGGATGTATAGATAGTGTCATAAGAAAGACTATCATAT197250.13336027808170248No Hit
GTCCAGATTACCGTTTTAGTTCAATATTCTTGATCCCTATGGTATCAAGGT169490.11459180497879724No Hit
AAATCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAG157740.10664765660130672No Hit
CATGCTTAGGCTAATCTATTTGAGTTTAGCTTGACTATTCCCCAATTAGC155000.10479514880944935No Hit
CGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAACGCAGG153920.10406496325645448No Hit
ATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAACGCAGG153440.10374043634401232No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)