FastQCFastQC Report
Fri 11 Jun 2021
5_S15_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5_S15_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17866396
Sequences flagged as poor quality0
Sequence length35-51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAAT16961129.493307995636053No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5869843.2854079804343304No Hit
ATCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTA5756943.2222167246264997No Hit
GCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCC3836312.147220961630986No Hit
GGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATT3732542.089139857865011No Hit
AGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATC2992771.6750832120814967No Hit
TATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAA2596561.4533205241840603No Hit
AGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTT2588931.4490499370997934No Hit
GCTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTAT2342211.310958292875631No Hit
CATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATGCG1673710.9367921767770064No Hit
GGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAACGC1457420.8157325069924566No Hit
CCACCAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAA1267360.7093540297662718No Hit
CTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATG1209850.6771651092923273No Hit
GTCACTTATACAATATACTTAGAAGCTTTTCCTGGAAGCATGGTATCAAT1192350.6673701847871277No Hit
ATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAACG1163180.6510434449118893No Hit
GCCCCGTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTAT1115300.6242445314656633No Hit
CACCAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAA1019070.5703836408865001No Hit
CACTTATACAATATACTTAGAAGCTTTTCCTGGAAGCATGGTATCAATAG802730.44929598560336403No Hit
CTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCCAC796640.4458873518755545No Hit
CCCACCAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGA794860.44489106812588286No Hit
GCAGGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGAT723840.40514046593392417No Hit
CCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATACGGGA711720.3983567810766088No Hit
GTCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTA632690.3541229020111275No Hit
GTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATACGGG619770.34689144917643155No Hit
ATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATGCGGTATT618450.3461526320137536No Hit
CTCAAATCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACT605930.33914506316774795No Hit
CCCCGTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATA572770.3205850805053241No Hit
GCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTT562420.3147920822979632No Hit
CTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCA493140.2760153754568073No Hit
GGTCACTTATACAATATACTTAGAAGCTTTTCCTGGAAGCATGGTATCAAT427790.23943832880453336No Hit
CAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCC404880.22661537335229778No Hit
GCCACCTTTAATCCACAGATATTATGCGGTATTAACAGTCGTTTCCAA375450.2101431088844107No Hit
TCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAAT373350.20896771794378677No Hit
ATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATGCGG363870.20366166741182723No Hit
CCCGTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATACG360760.2019209693997603No Hit
GTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATACGGGACTATCACC349810.195792145209364No Hit
TGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAACG344930.1930607605473426No Hit
AATCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCT329490.18441883858389796No Hit
CTTTATACTCTTGGCACGATTTCCGACCGTACCGAGTGCACCTTCGTACT328520.18387591991132404No Hit
CCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATGCGGT305900.1712152803508889No Hit
ATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAACGCAGG255860.14320739336573532No Hit
GTCCAGATTACCGTTTTAGTTCAATATTCTTGATCCCTATGGTATCAAGGT233170.13050757410727937No Hit
CGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAACGCAGG233120.1304795886086931No Hit
CCTTTATACTCTTGGCACGATTTCCGACCGTACCGAGTGCACCTTCGTA223740.12522950907390612No Hit
CTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATACGGGACT215750.12075742639981785No Hit
AAATCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAG203510.11390657634589539No Hit
CTTTTTTGTTGCCAGTTATTATTGGCATTTGTACTGTTATTAACGAGGTT199770.1118132610516413No Hit
CGGTCACTTATACAATATACTTAGAAGCTTTTCCTGGAAGCATGGTATCA185350.10374224325935685No Hit
GCCTTTATACTCTTGGCACGATTTCCGACCGTACCGAGTGCACCTTCGTA184720.10338962597716966No Hit
GCTTTTTTGTTGCCAGTTATTATTGGCATTTGTACTGTTATTAACGAGGTT184360.1031881303873484No Hit
GCTTAGGCTAATCTATTTGAGTTTAGCTTGACTATTCCCCAATTAGCCAGG179400.10041196892758898No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)