FastQCFastQC Report
Fri 11 Jun 2021
4_S14_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4_S14_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13483004
Sequences flagged as poor quality0
Sequence length35-51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAAT7834655.810759976040948No Hit
GCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCC2618141.9418076268463615No Hit
ATCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTA1974891.4647255166578605No Hit
AGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATC1802721.3370314211877412No Hit
TATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAA1271260.9428611012798038No Hit
AGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTT1257950.9329894139317915No Hit
GGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATT1234960.9159383176034064No Hit
GGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAACGC903430.6700509767704586No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN855760.6346953542400492No Hit
GCTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTAT720530.5343987141144511No Hit
ATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAACG664020.4924866891680815No Hit
CTTTTTTGTTGCCAGTTATTATTGGCATTTGTACTGTTATTAACGAGGTT645480.4787360442821199No Hit
CATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATGCG576180.42733800271808864No Hit
GCCCCGTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTAT493170.3657716040134676No Hit
GTCACTTATACAATATACTTAGAAGCTTTTCCTGGAAGCATGGTATCAAT492140.3650076792975809No Hit
GCTTTTTTGTTGCCAGTTATTATTGGCATTTGTACTGTTATTAACGAGGTT477450.3541124811651765No Hit
CCACCAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAA442240.32799812267355255No Hit
CACCAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAA408520.30298885915927937No Hit
CTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCA388900.2884372058333588No Hit
CTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATG379890.2817547187555533No Hit
GCTTAGGCTAATCTATTTGAGTTTAGCTTGACTATTCCCCAATTAGCCAGG321760.23864118114924537No Hit
GTCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTA316690.23488089152832706No Hit
GCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTT311230.23083134885964582No Hit
CACTTATACAATATACTTAGAAGCTTTTCCTGGAAGCATGGTATCAATAG305900.22687822387355222No Hit
CTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCCAC252110.1869835535167089No Hit
CCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATACGGGA249270.1848771979894095No Hit
CTCAAATCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACT232200.17221681459116975No Hit
CCCACCAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGA229830.17045904607014875No Hit
GTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATACGGG221260.16410289576417836No Hit
ATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATGCGGTATT218480.16204104070576555No Hit
CCCCGTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATA211830.1571089054041666No Hit
CCCAGACAAAAAGCAAAGACCGTCTTATGCGGAATATGAACGGATGTAT211350.15675290165307376No Hit
GTCCAGATTACCGTTTTAGTTCAATATTCTTGATCCCTATGGTATCAAGGT209900.15567747365498075No Hit
GCGGAATATGAACGGATGTATAGATAGTGTCATAAGAAAGACTATCATAT204600.15174659890333045No Hit
GCAGGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGAT198840.14747455389021616No Hit
GGGGTTTACAATGCCATTGAGTGTTACCACCAATGCGGTGCGCTCTTACC161430.11972851153941658No Hit
CAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCC154900.11488537717559083No Hit
TGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAACG154820.11482604321707537No Hit
GGTCACTTATACAATATACTTAGAAGCTTTTCCTGGAAGCATGGTATCAAT151100.1120670141461057No Hit
GTTTGCTTTTTTGTTGCCAGTTATTATTGGCATTTGTACTGTTATTAACG144540.10720162954783667No Hit
CCCGTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATACG143440.10638578761824886No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)