FastQCFastQC Report
Fri 11 Jun 2021
2_S12_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2_S12_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13361136
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAAT6764245.062623417649517No Hit
GCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCC5285433.9558238161785044No Hit
AGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATC2245181.6803810693940995No Hit
GGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATT1647191.232821819941059No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1590891.1906846842963055No Hit
AGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTT1590541.190422730522315No Hit
TATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAA1523751.1404344660513897No Hit
ATCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTA1444181.0808811466330408No Hit
GGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAACGC1404531.0512055262366913No Hit
GCTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTAT970840.7266148626883223No Hit
ATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAACG845570.63285786478036No Hit
GCCCCGTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTAT844430.6320046439165053No Hit
CTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCA812940.6084362886509052No Hit
CATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATGCG700020.5239225167680354No Hit
GTCACTTATACAATATACTTAGAAGCTTTTCCTGGAAGCATGGTATCAAT594850.44520914988066884No Hit
GCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTT549050.4109306274556295No Hit
CACCAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAA520640.38966746540114555No Hit
CCACCAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAA505430.3782837028228737No Hit
CTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATG403030.3016435129468033No Hit
CTTTTTTGTTGCCAGTTATTATTGGCATTTGTACTGTTATTAACGAGGTT393700.2946605737715715No Hit
CCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATACGGGA370230.27709470212712456No Hit
GCTTTTTTGTTGCCAGTTATTATTGGCATTTGTACTGTTATTAACGAGGTT342120.2560560718789181No Hit
CACTTATACAATATACTTAGAAGCTTTTCCTGGAAGCATGGTATCAATAG323380.2420303183801138No Hit
ATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATGCGGTATT319170.23887938869868552No Hit
GTCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTA316300.2367313677519636No Hit
CTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCCAC288700.216074441574429No Hit
GCAGGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGAT283630.2122798540483384No Hit
CCCCGTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATA282080.2111197730492377No Hit
GTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATACGGG281610.21076800655273625No Hit
GGTCACTTATACAATATACTTAGAAGCTTTTCCTGGAAGCATGGTATCAAT248170.18574019454633198No Hit
CCCACCAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGA231500.17326371051084277No Hit
CCCGTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATACG219450.16424501629202787No Hit
GTCCAGATTACCGTTTTAGTTCAATATTCTTGATCCCTATGGTATCAAGGT187280.14016772226553192No Hit
ATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATGCGG179230.134142785463751No Hit
CAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCC175570.13140349742716487No Hit
GCTTAGGCTAATCTATTTGAGTTTAGCTTGACTATTCCCCAATTAGCCAGG173260.12967460251882773No Hit
GCCACCTTTAATCCACAGATATTATGCGGTATTAACAGTCGTTTCCAA167040.12501930973533987No Hit
CTCAAATCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACT162400.12154655113158043No Hit
CCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATGCGGT156440.11708585258019977No Hit
AATCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCT155690.11652452306450589No Hit
GTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAACGCAGGCT139360.10430250840946459No Hit
ACCAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAG136850.10242392563027576No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)