FastQCFastQC Report
Fri 11 Jun 2021
1_S11_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1_S11_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12944723
Sequences flagged as poor quality0
Sequence length35-51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCATTTGAGGGGGATACCAGTTGGAAACGACTGTTAATACCGCATAAT6890285.322848546083218No Hit
AATCTGCCCATTTGAGGGGGATACCAGTTGGAAACGACTGTTAATACCGC4067983.1425778674445173No Hit
GGAATCTGCCCATTTGAGGGGGATACCAGTTGGAAACGACTGTTAATACCG4024583.109050691930604No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1671701.2914142697375601No Hit
GGGGGATACCAGTTGGAAACGACTGTTAATACCGCATAATATCTGTGGATT1657431.28039047262734No Hit
GGGGATACCAGTTGGAAACGACTGTTAATACCGCATAATATCTGTGGATT1642621.2689495171121081No Hit
GAATCTGCCCATTTGAGGGGGATACCAGTTGGAAACGACTGTTAATACCG1428451.1034998585910258No Hit
CTGCCCATTTGAGGGGGATACCAGTTGGAAACGACTGTTAATACCGCATA1312101.0136176726222725No Hit
ATCTGCCCATTTGAGGGGGATACCAGTTGGAAACGACTGTTAATACCGCAT1311991.0135326959101405No Hit
TCTGCCCATTTGAGGGGGATACCAGTTGGAAACGACTGTTAATACCGCAT1289990.9965373534837323No Hit
GTTGGAAACGACTGTTAATACCGCATAATATCTGTGGATTAAAGGTGGCTT1149430.8879525656902816No Hit
GGGATACCAGTTGGAAACGACTGTTAATACCGCATAATATCTGTGGATTA994390.7681817525179953No Hit
CCCATTTGAGGGGGATACCAGTTGGAAACGACTGTTAATACCGCATAATAT618910.4781176082331001No Hit
TGCCCATTTGAGGGGGATACCAGTTGGAAACGACTGTTAATACCGCATAAT432260.33392757805632456No Hit
AGGAATCTGCCCATTTGAGGGGGATACCAGTTGGAAACGACTGTTAATAC429410.3317259086965399No Hit
GGTAGGAATGCTAGGCAAATCCGGTGTTCTGTTGATCTGAGATACGAGACG411680.31802920773198473No Hit
GGATACCAGTTGGAAACGACTGTTAATACCGCATAATATCTGTGGATTAA354900.2741657739605552No Hit
AGTTGGAAACGACTGTTAATACCGCATAATATCTGTGGATTAAAGGTGG354370.2737563407111917No Hit
GCTAGGCAAATCCGGTGTTCTGTTGATCTGAGATACGAGACGAAGTTAAA349130.26970835915144725No Hit
GGTTTCGACATGAATGTCAAAATCTAAGGTGCATGCCGAGGAAGTACCGT345400.26682687609460626No Hit
ATTTAGCTGTAATTATAGTAGAACAAGTTGGGAAGCTTGACGATAGAGGGT341720.2639840188160071No Hit
GCCTGGTATGATTTAGCTGTAATTATAGTAGAACAAGTTGGGAAGCTTGA314870.24324197590014093No Hit
GTTTCGACATGAATGTCAAAATCTAAGGTGCATGCCGAGGAAGTACCGTA311900.24094760467257584No Hit
GGCAAATCCGGTGTTCTGTTGATCTGAGATACGAGACGAAGTTAAACTTGT306700.23693052373542486No Hit
TTAGCTGTAATTATAGTAGAACAAGTTGGGAAGCTTGACGATAGAGGGTG254440.19655886031705738No Hit
GTATGATTTAGCTGTAATTATAGTAGAACAAGTTGGGAAGCTTGACGAT237450.18343382087048135No Hit
GTTGATCTGAGATACGAGACGAAGTTAAACTTGTTTGACGAAGCTATTGAT231980.17920816073082446No Hit
GGAAACGACTGTTAATACCGCATAATATCTGTGGATTAAAGGTGGCTTTCG219850.1698375469293549No Hit
TTTAGCTGTAATTATAGTAGAACAAGTTGGGAAGCTTGACGATAGAGGGTG219650.16968304381638757No Hit
CAGTTGGAAACGACTGTTAATACCGCATAATATCTGTGGATTAAAGGTGG218880.1690882068314633No Hit
TGGAAACGACTGTTAATACCGCATAATATCTGTGGATTAAAGGTGGCTTT213420.16487027184745476No Hit
GATTTAGCTGTAATTATAGTAGAACAAGTTGGGAAGCTTGACGATAGAGGG178470.13787085285641107No Hit
GATACCAGTTGGAAACGACTGTTAATACCGCATAATATCTGTGGATTAA175650.13569235896357149No Hit
ACCAGTTGGAAACGACTGTTAATACCGCATAATATCTGTGGATTAAAGGTG166060.12828393469678726No Hit
AGTAGAACAAGTTGGGAAGCTTGACGATAGAGGGTGATAGTCCCGTATACG164210.12685478090183933No Hit
CTTGACGATAGAGGGTGATAGTCCCGTATACGAAATAATCACAGTGGAA146450.11313490447033898No Hit
GGAAGCTTGACGATAGAGGGTGATAGTCCCGTATACGAAATAATCACAGTG144300.11147399600594003No Hit
GGAATGCTAGGCAAATCCGGTGTTCTGTTGATCTGAGATACGAGACGAAGT141290.10914872415578147No Hit
TGCTAGGCAAATCCGGTGTTCTGTTGATCTGAGATACGAGACGAAGTTAA140870.10882426761855005No Hit
AGAACAAGTTGGGAAGCTTGACGATAGAGGGTGATAGTCCCGTATACGAA131610.10167077348816192No Hit
ATTTGAGGGGGATACCAGTTGGAAACGACTGTTAATACCGCATAATAT131310.10143901881871091No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)