FastQCFastQC Report
Fri 11 Jun 2021
12_S22_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename12_S22_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12873569
Sequences flagged as poor quality0
Sequence length35-51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAAT7263345.642056216112254No Hit
GCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCC2485421.9306378829367365No Hit
ATCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTA1907541.4817491559644416No Hit
AGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATC1831421.4226202539482253No Hit
GGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATT1466781.1393732382993402No Hit
AGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTT1350491.0490408681539674No Hit
TATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAA1215910.9445010936749553No Hit
GGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAACGC890960.6920846891798226No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN839870.6523987248602156No Hit
GCTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTAT713360.5541276082801903No Hit
CATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATGCG705550.5480609145762143No Hit
GCCCCGTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTAT664320.5160340539597061No Hit
CTTTTTTGTTGCCAGTTATTATTGGCATTTGTACTGTTATTAACGAGGTT572700.44486497877938896No Hit
GTCACTTATACAATATACTTAGAAGCTTTTCCTGGAAGCATGGTATCAAT565210.4390468563923493No Hit
ATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAACG557760.43325980541992665No Hit
CCACCAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAA492410.3824968817893468No Hit
CTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCA473650.36792438833395774No Hit
CACCAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAA447630.3477124331255769No Hit
GCTTTTTTGTTGCCAGTTATTATTGGCATTTGTACTGTTATTAACGAGGTT430000.334017707133119No Hit
CTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATG390980.30370754217420204No Hit
CCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATACGGGA319420.2481207814243276No Hit
GTCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTA282500.2194418657328049No Hit
CCCACCAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGA282290.2192787408060655No Hit
CACTTATACAATATACTTAGAAGCTTTTCCTGGAAGCATGGTATCAATAG281880.21896025880624093No Hit
GCTTAGGCTAATCTATTTGAGTTTAGCTTGACTATTCCCCAATTAGCCAGG277830.2158142780762662No Hit
CTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCCAC275590.21407427885771227No Hit
CCCCGTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATA270600.21019811988423726No Hit
GCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTT264010.20507910432608084No Hit
GTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATACGGG263150.20441106891181457No Hit
ATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATGCGGTATT259860.20185544505956352No Hit
CCCAGACAAAAAGCAAAGACCGTCTTATGCGGAATATGAACGGATGTAT226530.17596518882991966No Hit
CCCGTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATACG212640.1651756401041545No Hit
GCAGGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGAT212110.1647639438604788No Hit
GGTCACTTATACAATATACTTAGAAGCTTTTCCTGGAAGCATGGTATCAAT210370.1634123373246378No Hit
CTCAAATCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACT204100.15854189308341765No Hit
GTCCAGATTACCGTTTTAGTTCAATATTCTTGATCCCTATGGTATCAAGGT202410.15722912581584797No Hit
ATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATGCGG168310.1307407448548262No Hit
CAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCC166490.12932699548975113No Hit
GCCACCTTTAATCCACAGATATTATGCGGTATTAACAGTCGTTTCCAA163010.12662378241806915No Hit
GGGGTTTACAATGCCATTGAGTGTTACCACCAATGCGGTGCGCTCTTACC161820.12569940783321237No Hit
GTTTGCTTTTTTGTTGCCAGTTATTATTGGCATTTGTACTGTTATTAACG153540.11926762500748626No Hit
CCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATGCGGT149950.11647896554560745No Hit
AATCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCT144710.11240861023077593No Hit
GGATCTACATGCTTAGGCTAATCTATTTGAGTTTAGCTTGACTATTCCCC140760.1093403080373438No Hit
GCGGAATATGAACGGATGTATAGATAGTGTCATAAGAAAGACTATCATAT138700.10774013018456655No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)