FastQCFastQC Report
Fri 11 Jun 2021
11_S21_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename11_S21_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13076839
Sequences flagged as poor quality0
Sequence length35-51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAAT6826645.22040532884132No Hit
GCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCC2346481.7943785956223826No Hit
ATCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTA2002931.5316622006281488No Hit
AGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATC1657911.2678216807593945No Hit
AGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTT1266130.968223283929702No Hit
GGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATT1169980.8946963406064722No Hit
TATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAA1043850.7982433675294159No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN869570.6649695694808203No Hit
GGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAACGC771910.5902879128511103No Hit
GTCACTTATACAATATACTTAGAAGCTTTTCCTGGAAGCATGGTATCAAT682960.5222668872806341No Hit
GCTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTAT642910.49164021978094247No Hit
GCCCCGTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTAT592420.4530299715397582No Hit
CTTTTTTGTTGCCAGTTATTATTGGCATTTGTACTGTTATTAACGAGGTT535910.40981616428863277No Hit
CATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATGCG513940.3930154680347445No Hit
ATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCAACG488420.37350004844442913No Hit
GCTTTTTTGTTGCCAGTTATTATTGGCATTTGTACTGTTATTAACGAGGTT462750.3538699222342647No Hit
CCACCAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAA428940.32801505012029286No Hit
CTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTAATCCA396420.30314665493702264No Hit
CACCAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAA386850.29582837259065436No Hit
CACTTATACAATATACTTAGAAGCTTTTCCTGGAAGCATGGTATCAATAG322350.24650452605557044No Hit
CTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATG316350.24191626126160917No Hit
GTCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCTA303110.23179149028293458No Hit
CCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATACGGGA297090.22718793127299342No Hit
CTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCCAC271050.2072748620672014No Hit
CTCAAATCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACT263780.2017154145585183No Hit
GCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTT260510.19921481024580942No Hit
CCCACCAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGA254410.19455007437194877No Hit
GGTCACTTATACAATATACTTAGAAGCTTTTCCTGGAAGCATGGTATCAAT251030.19196535187135055No Hit
CCCATATTCAGACAAGGTTCCACGTGCCCCGTCCTACTTGTTCGTATGCTT244040.18662002338638567No Hit
GTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATACGGG243350.1860923729350801No Hit
GCTTAGGCTAATCTATTTGAGTTTAGCTTGACTATTCCCCAATTAGCCAGG227700.17412464893083104No Hit
CCCCGTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATA225220.17222816614932707No Hit
GTCCAGATTACCGTTTTAGTTCAATATTCTTGATCCCTATGGTATCAAGGT212390.16241692659823984No Hit
ATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGATATTATGCGGTATT195950.1498450810627859No Hit
CCCAGACAAAAAGCAAAGACCGTCTTATGCGGAATATGAACGGATGTAT182790.13978148694803078No Hit
CCCGTCCTACTTGTTCGTATGCTTAGTTCCACTGTGATTATTTCGTATACG172580.13197378969030665No Hit
GCAGGCTCATCCATCTGCGACAGCCCGAAAGCCACCTTTAATCCACAGAT162670.12439550567228058No Hit
GTTTGCTTTTTTGTTGCCAGTTATTATTGGCATTTGTACTGTTATTAACG156230.11947076812676212No Hit
CAACTAGCTAATCCAACGCAGGCTCATCCATCTGCGACAGCCCGAAAGCC153070.11705428200194251No Hit
AATCAGCTATGGATCGTAGCCTTGGTGGGCCCTTACCCCACCAACTAGCT146580.11209130891647438No Hit
GCGGAATATGAACGGATGTATAGATAGTGTCATAAGAAAGACTATCATAT142150.10870364007693296No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)