##BLOCKS= 7 Note: PROTOCOL REVISION HISTORY: v1.0.0: original protocol created (MDC) v1.0.1: 06/30/05 - Updated, spell checked, & formatted to new style guide. (DW) READER SUITABILITY: EMax, VMax, ThermoMax, VersaMax, SpectraMax, SpectraMax Plus, SpectraMax Plus 384, SpectraMax 190, SpectraMax 340PC, SpectraMax 340PC 384, SpectraMax M2, SpectraMax M5 ~End Note: Basic Endpoint Protocol Unfamiliar with SoftMax Pro software? If you are new to SoftMax Pro software, we strongly recommended spending time familiarizing yourself with the powerful features by completing at least one of the tutorials located in the Tutorials folder. Protocol Use Use this protocol for endpoint assays that have unknowns that will have concentrations interpolated from a standard curve. Modify the instrument setup for the wavelength(s) of interest for your assay. You may also modify the template to include additional standards, unknowns, and controls. ~End Plate: Plate01 1.3 PlateFormat Endpoint Fluorescence FALSE Raw FALSE 1 1 550 9 2 96 510 Automatic 550 3 Automatic 1 5 None Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 23.40 2500.267 2065.829 1859.406 1768.066 1248.547 1031.287 596.926 573.591 28.06 30.746 ~End Group: Standards 1 Sample Conc BackCalcConc Wells Value MeanValue SD CV Smallest standard value: Largest standard value: ~End Group: Unknowns 1 Sample Wells Value R Result MeanResult SD CV R - Outside standard range ~End Group: Unk_Dilution 1 Sample Wells Value R Result MeanResult SD CV Dilution AdjResult R - Outside standard range Mean Adjusted Result: ~End Group: Control 1 Sample Wells Sample# Values MeanValue ~End Original Filename: Untitled Date Last Saved: Unsaved